CN115844804B - Composition for improving exogenous aging and preparation method and application thereof - Google Patents
Composition for improving exogenous aging and preparation method and application thereof Download PDFInfo
- Publication number
- CN115844804B CN115844804B CN202211494327.XA CN202211494327A CN115844804B CN 115844804 B CN115844804 B CN 115844804B CN 202211494327 A CN202211494327 A CN 202211494327A CN 115844804 B CN115844804 B CN 115844804B
- Authority
- CN
- China
- Prior art keywords
- extract
- parts
- aging
- improving
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 80
- 230000032683 aging Effects 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 239000000284 extract Substances 0.000 claims abstract description 157
- 241000272503 Sparassis radicata Species 0.000 claims abstract description 40
- 108010058643 Fungal Proteins Proteins 0.000 claims abstract description 38
- 239000002537 cosmetic Substances 0.000 claims abstract description 35
- 235000012253 Rosa stellata subsp abyssa Nutrition 0.000 claims abstract description 34
- 235000007072 Rosa stellata subsp mirifica Nutrition 0.000 claims abstract description 34
- 235000001634 Rosa stellata subsp stellata Nutrition 0.000 claims abstract description 34
- 235000004483 Rosa stellata var. erlansoniae Nutrition 0.000 claims abstract description 34
- 235000010976 Rosa stellata var. mirifica Nutrition 0.000 claims abstract description 34
- 241000218176 Corydalis Species 0.000 claims abstract description 31
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 44
- 239000000047 product Substances 0.000 claims description 31
- 239000000839 emulsion Substances 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 20
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims description 20
- 230000001815 facial effect Effects 0.000 claims description 18
- 230000003712 anti-aging effect Effects 0.000 claims description 17
- 239000002994 raw material Substances 0.000 claims description 13
- 239000008367 deionised water Substances 0.000 claims description 12
- 229910021641 deionized water Inorganic materials 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 10
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 10
- 229940015975 1,2-hexanediol Drugs 0.000 claims description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 10
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 10
- JMBINOWGIHWPJI-UNSOMVRXSA-N [(2r,3r,4r,5r,6r)-2-[[(2r,3r,4r)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxymethyl]-6-[2-(3,4-dihydroxyphenyl)ethoxy]-5-hydroxy-4-[(2s,3r,4r,5r,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-3-yl] (e)-3-(3,4-dihydroxyphenyl)prop-2-enoate Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@@H](CO[C@H]2[C@@H]([C@@](O)(CO)CO2)O)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O JMBINOWGIHWPJI-UNSOMVRXSA-N 0.000 claims description 10
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 10
- 235000019437 butane-1,3-diol Nutrition 0.000 claims description 10
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 10
- 229920001285 xanthan gum Polymers 0.000 claims description 10
- 239000000230 xanthan gum Substances 0.000 claims description 10
- 229940082509 xanthan gum Drugs 0.000 claims description 10
- 235000010493 xanthan gum Nutrition 0.000 claims description 10
- DAUAQNGYDSHRET-UHFFFAOYSA-N 3,4-dimethoxybenzoic acid Chemical compound COC1=CC=C(C(O)=O)C=C1OC DAUAQNGYDSHRET-UHFFFAOYSA-N 0.000 claims description 9
- GPVDHNVGGIAOQT-UHFFFAOYSA-N Veratric acid Natural products COC1=CC=C(C(O)=O)C(OC)=C1 GPVDHNVGGIAOQT-UHFFFAOYSA-N 0.000 claims description 9
- 241001149258 Sporobolus alterniflorus Species 0.000 claims description 8
- 239000008213 purified water Substances 0.000 claims description 8
- 239000004480 active ingredient Substances 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 7
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 6
- OLGWXCQXRSSQPO-MHARETSRSA-N P(1),P(4)-bis(5'-guanosyl) tetraphosphate Chemical compound C1=NC(C(NC(N)=N2)=O)=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(=O)OP(O)(=O)OC[C@H]([C@@H](O)[C@H]1O)O[C@H]1N1C(N=C(NC2=O)N)=C2N=C1 OLGWXCQXRSSQPO-MHARETSRSA-N 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000000413 hydrolysate Substances 0.000 claims description 6
- 230000002829 reductive effect Effects 0.000 claims description 6
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 5
- 229920002498 Beta-glucan Polymers 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- 240000004713 Pisum sativum Species 0.000 claims description 5
- 235000010582 Pisum sativum Nutrition 0.000 claims description 5
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 229930003268 Vitamin C Natural products 0.000 claims description 5
- 229930003427 Vitamin E Natural products 0.000 claims description 5
- 239000002671 adjuvant Substances 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 5
- 239000006285 cell suspension Substances 0.000 claims description 5
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- JMBINOWGIHWPJI-UHFFFAOYSA-N pedicularoside A Natural products OC1C(O)C(O)C(C)OC1OC1C(OC(=O)C=CC=2C=C(O)C(O)=CC=2)C(COC2C(C(O)(CO)CO2)O)OC(OCCC=2C=C(O)C(O)=CC=2)C1O JMBINOWGIHWPJI-UHFFFAOYSA-N 0.000 claims description 5
- 230000009758 senescence Effects 0.000 claims description 5
- 239000011718 vitamin C Substances 0.000 claims description 5
- 235000019154 vitamin C Nutrition 0.000 claims description 5
- 239000011709 vitamin E Substances 0.000 claims description 5
- 235000019165 vitamin E Nutrition 0.000 claims description 5
- 229940046009 vitamin E Drugs 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 4
- OLGWXCQXRSSQPO-UHFFFAOYSA-N diguanosine tetraphosphate Natural products C1=NC(C(N=C(N)N2)=O)=C2N1C(C(O)C1O)OC1COP(O)(=O)OP(O)(=O)OP(O)(=O)OP(O)(=O)OCC(C(O)C1O)OC1N1C=NC2=C1NC(N)=NC2=O OLGWXCQXRSSQPO-UHFFFAOYSA-N 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 230000035755 proliferation Effects 0.000 claims description 4
- DTOUWTJYUCZJQD-QJDQKFITSA-N Forsythiaside Natural products C[C@@H]1O[C@H](OC[C@H]2O[C@@H](OCCc3ccc(O)c(O)c3)[C@H](O)[C@@H](O)[C@@H]2OC(=O)C=Cc4ccc(O)c(O)c4)[C@H](O)[C@H](O)[C@H]1O DTOUWTJYUCZJQD-QJDQKFITSA-N 0.000 claims description 3
- DTOUWTJYUCZJQD-UJERWXFOSA-N Forsythiaside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@H](O)[C@@H](O)[C@H](OCCC=2C=C(O)C(O)=CC=2)O1 DTOUWTJYUCZJQD-UJERWXFOSA-N 0.000 claims description 3
- 241000220317 Rosa Species 0.000 claims description 3
- 241000235070 Saccharomyces Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000002738 chelating agent Substances 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 239000003906 humectant Substances 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 239000002562 thickening agent Substances 0.000 claims description 3
- 241000746413 Spartina Species 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000003750 conditioning effect Effects 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 238000009928 pasteurization Methods 0.000 claims description 2
- 241001278834 Rosa stellata Species 0.000 claims 7
- 235000011203 Origanum Nutrition 0.000 claims 3
- 240000000783 Origanum majorana Species 0.000 claims 3
- 240000008042 Zea mays Species 0.000 claims 1
- 230000037303 wrinkles Effects 0.000 abstract description 34
- 230000000694 effects Effects 0.000 abstract description 31
- 241000983387 Adenium obesum Species 0.000 abstract description 30
- 239000011148 porous material Substances 0.000 abstract description 20
- 230000036548 skin texture Effects 0.000 abstract description 8
- 238000005282 brightening Methods 0.000 abstract description 7
- 230000009471 action Effects 0.000 abstract description 5
- 238000007254 oxidation reaction Methods 0.000 abstract description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 3
- 230000003647 oxidation Effects 0.000 abstract description 3
- 230000027288 circadian rhythm Effects 0.000 abstract description 2
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 231100000344 non-irritating Toxicity 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 84
- 230000000052 comparative effect Effects 0.000 description 33
- 210000004027 cell Anatomy 0.000 description 30
- 230000006872 improvement Effects 0.000 description 28
- 238000012360 testing method Methods 0.000 description 26
- 238000003756 stirring Methods 0.000 description 22
- 235000011187 glycerol Nutrition 0.000 description 12
- 238000010438 heat treatment Methods 0.000 description 8
- 230000002195 synergetic effect Effects 0.000 description 6
- 230000009759 skin aging Effects 0.000 description 5
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
- 235000011449 Rosa Nutrition 0.000 description 4
- 241000209149 Zea Species 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000007599 discharging Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 206010051246 Photodermatosis Diseases 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 230000008845 photoaging Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000033764 rhythmic process Effects 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000002431 foraging effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000003642 reactive oxygen metabolite Substances 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 230000005808 skin problem Effects 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 240000007440 Agaricus campestris Species 0.000 description 1
- 241000227752 Chaetoceros Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000579484 Homo sapiens Period circadian protein homolog 1 Proteins 0.000 description 1
- 101001126582 Homo sapiens Post-GPI attachment to proteins factor 3 Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000173878 Letharia Species 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 102100028293 Period circadian protein homolog 1 Human genes 0.000 description 1
- 208000010340 Sleep Deprivation Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- -1 conditioner Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000000887 face Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 231100000722 genetic damage Toxicity 0.000 description 1
- BUFLLCUFNHESEH-UUOKFMHZSA-N guanosine 3',5'-bis(diphosphate) Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](OP(O)(=O)OP(O)(O)=O)[C@H]1O BUFLLCUFNHESEH-UUOKFMHZSA-N 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 125000003473 lipid group Chemical group 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000036578 sleeping time Effects 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
The invention belongs to the field of cosmetics, and relates to a composition for improving exogenous aging, a preparation method and application thereof, wherein the composition comprises the following components in parts by weight: 10-40 parts of sparassis crispa extract, 10-40 parts of plankton extract, 10-40 parts of desert rose (ADENIUM OBESUM) leaf cell extract, 10-20 parts of European decumbent corydalis herb extract and 10-20 parts of hydrolyzed yeast protein. The composition for improving exogenous aging provided by the invention has the effects of repairing ultraviolet injury, resisting cold and hot aging, resisting oxidation, resisting pollution, regulating the circadian rhythm of skin and the like under the combined action of multiple components, can accurately solve exogenous aging caused by environment and bad effects in multiple directions, has the effects of improving the moisture content of skin, brightening skin color, reducing wrinkles, shrinking pores and improving skin texture, is safe and non-irritating, and can be widely applied to the cosmetic industry.
Description
Technical Field
The invention belongs to the technical field of daily chemicals, and particularly relates to a composition for improving exogenous aging, and a preparation method and application thereof.
Background
Skin aging is a complex process, affected by both endogenous and exogenous factors. The human body and the environment are a dense and inseparable whole, the skin is used as the outermost organ of the human body, plays roles in protecting, feeling, regulating body temperature, excreting and immunizing, and the like, and is directly contacted with the external environment, so that the skin is not challenged and invaded by environmental exposure factors at any time.
Skin aging is closely related to environmental exposure. Light and ROS (reactive oxygen species) cause DNA damage, apoptosis, and are major factors affecting skin aging, but in addition to this humans are also faced with increasingly extreme high temperature, low temperature weather, increasingly severe air pollution, and with night-stay irregularities. Skin temperature can be up and down floated by more than 20 ℃ according to the environment in the whole day, so that skin physiological damage can be caused, and researches show that temperature aging can cause enzyme inactivation and protein damage; studies have demonstrated that every 20.04 μg/m 3 increase in PM10 means an increase in 16% on-cheek pigmented spots (Nakamura, 2015; huls, 2016); investigation shows that the problems of dry and rough skin, easy rash due to rough skin, poor make-up effect and the like of people with irregular sleeping habits at night are respectively 1.4 times, 1.7 times and 1.7 times of people with regular sleeping time.
At present, many researches on improving skin aging are carried out, and the related technologies for improving skin aging have the following defects:
1. Mainly focuses on improving endogenous aging, but endogenous factors are greatly influenced by genes, hormones and the like, and the improvement in the cosmetic range is greatly restricted. External ageing of the skin is caused by several controllable factors and occurs with varying degrees of intensity. Thus, improving exogenous aging can achieve a great effect.
2. The human skin faces various environmental threats, and the technical specialists research on photo-aging resistance and oxidation resistance, however, the system comprehensively solves the problems of various environments and living habits and has little influence.
3. The efficacy of the prior art formulation is not comprehensive.
Therefore, a product with comprehensive anti-aging effect aiming at exogenous aging, comprehensive and systematic targets and comprehensive effects is needed to be developed.
Disclosure of Invention
In order to overcome the problems of the prior art, the invention provides a composition for improving exogenous aging, and a preparation method and application thereof.
The composition provided by the invention is complementary in action mechanism, and has the functions of resisting ultraviolet rays, resisting cold and hot ageing, resisting oxidation, resisting pollution, regulating skin rhythm and the like when being applied in a combined way, so that the effects of improving skin moisture content, brightening skin color, reducing wrinkles, shrinking pores and improving skin texture are achieved.
Meanwhile, the composition provided by the invention has the advantages of simple preparation process and strong operability, the stability of active ingredients can be maintained in the preparation process, and the composition for improving exogenous aging is used in the preparation of cosmetics or skin care products, so that the skin feel is fresh and easy to absorb when the cosmetics or skin care products are used, and the skin is not stimulated.
The aim of the invention can be achieved by the following technical scheme:
The invention firstly provides a composition for improving exogenous aging, which comprises the following components in parts by weight: 10-40 parts of sparassis crispa extract, 10-40 parts of plankton extract, 10-40 parts of desert rose (ADENIUM OBESUM) leaf cell extract, 10-20 parts of European decumbent corydalis herb extract and 10-20 parts of hydrolyzed yeast protein.
In a preferred embodiment of the invention, the exogenous aging-ameliorating composition comprises the following components in parts by weight: 10 parts of sparassis crispa extract, 40 parts of plankton extract, 20 parts of desert rose leaf cell extract, 10 parts of European decumbent corydalis herb extract and 20 parts of hydrolyzed yeast protein.
In a preferred embodiment of the invention, the exogenous aging-ameliorating composition comprises the following components in parts by weight: 40 parts of Sparassis crispa extract, 20 parts of plankton extract, 10 parts of desert rose leaf cell extract, 20 parts of European corydalis herb extract and 10 parts of hydrolyzed yeast protein.
In a preferred embodiment of the invention, the exogenous aging-ameliorating composition comprises the following components in parts by weight: 30 parts of sparassis crispa extract, 30 parts of plankton extract, 15 parts of desert rose leaf cell extract, 15 parts of European decumbent corydalis herb extract and 10 parts of hydrolyzed yeast protein.
In the embodiment of the invention, in addition to the spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european-grass extract and hydrolyzed yeast protein, the composition for improving exogenous aging can be added with a solvent and auxiliary materials which are allowed to be used in cosmetics or skin care products, wherein the solvent can be water, glycerol and the like, and the auxiliary materials can be one or more of surfactants, thickeners, conditioning agents, moisturizers, preservatives and chelating agents.
In the application, the sparassis crispa extract contains veratric acid, a certain amount of beta-glucan, vitamin C and vitamin E. The content of veratric acid in the Sparassis crispa extract has a certain relation with the specific obtaining mode of the Sparassis crispa extract, and the content of veratric acid in the Sparassis crispa extract is generally in the range of 1000-6000 ppm. For the purposes of the present application, preference is given to Sparassis crispa extracts having a veratric acid content in the range from 5000ppm to 6000 ppm.
In one embodiment of the invention, the sparassis crispa extract is prepared by: adding purified water into Sparassis crispa mycelium, shaking, mixing, shearing at high speed, extracting at high temperature, centrifuging to obtain supernatant, concentrating under reduced pressure to obtain Sparassis crispa extract containing 1000-6000ppm veratric acid, a certain amount of beta-glucan, vitamin C and vitamin E.
In the preparation process of the sparassis crispa extract, sparassis crispa mycelium is added into purified water according to a feed-to-liquid ratio of 1:10.
In the present invention, the plankton extract contains diguanosine tetraphosphate (GP 4G nucleotide).
In one embodiment of the present invention, the plankton extract is prepared by the following steps: adding purified water into the phaga polar plankton to obtain suspension, grinding, extracting, centrifuging, filtering, and low-temperature pasteurizing to obtain plankton extract containing diguanosine tetraphosphate (GP 4G nucleotide).
In the preparation process of plankton extract, adding the phagostimulant plankton into purified water according to a feed-liquid ratio of 1:10.
In one embodiment of the invention, the preparation method of the desert rose leaf cell extract comprises the following steps: selecting the callus of the desert rose She Shengcheng, carrying out biological amplification after suspension proliferation, purifying to obtain a cell suspension, extracting, filtering and sterilizing.
In the application, the extract of the European-summer-grass contains an active ingredient of forsythoside B, the content of the forsythoside B in the extract of the European-summer-grass has a certain relation with the specific obtaining mode of the extract of the European-summer-grass, and the content of the forsythoside B in the extract of the European-summer-grass is generally in the range of 200-600 ppm. For the purposes of the present application, preference is given to an extract of Letharia latifolia having a forsythoside B content in the range from 300ppm to 600ppm, for example chosen to be 350 ppm.
In one embodiment of the invention, the method for preparing the European decumbent corydalis herb extract comprises the following steps: selecting European corydalis tuber leaves to generate callus, carrying out biological amplification after suspension proliferation, purifying to obtain cell suspension, and extracting by an extraction process to obtain the European corydalis tuber extract containing 200-600ppm of forsythiaside B active ingredient.
In one embodiment of the invention, the hydrolyzed yeast protein is derived from a hydrolysate of a plant such as single grain wheat (Triticummonococum), potato (Solanum tuberosum), corn (Zea mayz l.) or pea (Pisum sativum) or a hydrolysate of yeast from the genus saccharomyces, obtained by fermentation techniques. The hydrolyzed yeast protein contains peptides with molecular weight less than 6kDa by purification.
The invention further provides a preparation method of the composition for improving exogenous aging, which comprises the steps of fully mixing a certain amount of spartina crispa extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein, and uniformly stirring to obtain the composition for improving exogenous aging.
In one embodiment of the invention, the temperature of the mixing is 10-30 ℃ during the preparation of the exogenous senescence-improving composition. The stirring speed is 100-200r/min.
The invention further provides the use of the exogenous senescence-improving composition in the preparation of a cosmetic or skin care product comprising from 0.01 to 50% of the exogenous senescence-improving composition, based on the total weight of the cosmetic or skin care product; and 50-99.99% of cosmetic or skin care product solvent and adjuvants; preferably, the cosmetic or skin care product comprises 0.01-30% of the exogenous aging-improving composition; and 70-99.99% of cosmetic or skin care product solvent and adjuvants. In one embodiment of the present invention, the auxiliary material comprises one or more of surfactant, thickener, conditioner, humectant, preservative and chelating agent. The solvent includes deionized water, glycerol, etc.
In one embodiment of the invention, the cosmetics or skin care products are made into various cosmetics or skin care products such as anti-aging facial cleanser, anti-aging facial cream, anti-aging emulsion, anti-aging toning lotion, anti-aging facial mask, anti-aging eye cream or anti-aging gel.
The present invention still further provides a cosmetic or skin care product for improving exogenous aging, comprising 0.01-50% of the exogenous aging-improving composition, based on the total weight of the cosmetic or skin care product; and 50-99.99% of cosmetic or skin care product solvent and adjuvants.
In one embodiment of the invention, the skin care product for improving exogenous aging is an aging-delaying emulsion, and the raw material components of the aging-delaying emulsion comprise, by weight: 0.01-50% of composition for improving exogenous aging, 0.2% of xanthan gum, 3% of 1, 3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1, 2-hexanediol and the balance of deionized water. Preferably, the raw material components of the anti-aging emulsion comprise: 0.01-30% of composition for improving exogenous aging, 0.2% of xanthan gum, 3% of 1, 3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1, 2-hexanediol and the balance of deionized water. The anti-aging emulsion may comprise 0.01%,1%,5%,10%,15%,20%,25%,30%, etc. of the exogenous aging-improving composition.
Further, the anti-aging emulsion is prepared by the following method:
Pre-dispersing 1, 3-butanediol, EDTA-2Na and xanthan gum uniformly, adding into deionized water, and stirring until the mixture is completely dissolved; adding trehalose, stirring at 200-400r/min to disperse uniformly, heating to 80-90deg.C, adding p-hydroxyacetophenone, stirring uniformly, and stopping heating after completely dissolving; cooling to 38-42 ℃, adding the composition for improving exogenous aging, stirring uniformly glycerol and 1, 2-hexanediol, adding into a system, stirring uniformly, discharging and subpackaging to obtain the aging-delaying emulsion.
Compared with the prior art, the invention has the beneficial effects that:
1) The composition for improving exogenous aging provided by the invention simultaneously comprises the sparassis crispa extract, the plankton extract, the desert rose leaf cell extract, the European-grass extract and the hydrolyzed yeast protein, and the components are combined for application, so that the composition has the functions of resisting ultraviolet rays, resisting cold and hot aging, resisting oxidization, resisting pollution and regulating skin rhythm, and comprehensively solves the problem of exogenous aging, thereby achieving the multiple effects of improving the skin moisture content, brightening skin color, reducing wrinkles, shrinking pores and improving skin texture.
2) The composition for improving the exogenous aging provided by the invention has the advantages of simple preparation process and strong operability, can keep the stability of active ingredients in the preparation process, is used for preparing cosmetics or skin care products, and has fresh and easy absorption skin feel and does not cause irritation to skin when the cosmetics or skin care products are used. Therefore, the method has important practical significance and wide market prospect.
Drawings
FIG. 1 is a graph of facial texture improvement for an exogenous aging-improving composition provided by the present invention.
Fig. 2 is a diagram showing improvement of facial wrinkles in the composition for improving exogenous aging provided by the present invention.
FIG. 3 is a graph of improvement in facial skin tone for an exogenous aging-improving composition provided by the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.
The invention discloses a composition for improving exogenous aging, which comprises spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein.
The following technical effects of the raw material components are respectively described.
The following focuses on the preparation method and efficacy description of each raw material component in the composition.
① Sparassis crispa extract
The preparation method comprises the following steps: weighing Sparassis crispa mycelium, adding purified water according to a feed-liquid ratio of 1:10, shaking and mixing uniformly, shearing at high speed, extracting at high temperature, centrifuging to obtain supernatant, and concentrating under reduced pressure to obtain Sparassis crispa extract containing 5000ppm veratric acid;
efficacy: the common mushrooms grow on the shade, and sparassis crispa needs more than 10 hours of irradiation every day, so that the sparassis crispa is the only 'sun mushrooms' in the world. The Sparassis crispa extract contains 5000ppm veratric acid, and has effects of resisting photoaging, inhibiting UVB-induced inflammation, and protecting UVB; sparassis crispa also has good efficacy for removing skin problems such as melanin precipitation and the like because of containing a large amount of beta glucan, antioxidant substances, vitamin C and vitamin E.
② Plankton extract
The preparation method comprises the following steps: weighing phagostimulant plankton (such as blue algae or green algae), adding purified water according to a feed liquid ratio of 1:10 to obtain suspension, grinding, extracting, centrifuging, filtering, and performing low-temperature pasteurization to obtain plankton extract containing guanosine tetraphosphate (GP 4G nucleotide).
Efficacy: during evolution, this plankton has been adapted to survive in severe environments at extreme temperatures, with GP4G nucleotides being the core element of the overall adaptation system, and plankton can convert GP4G to ATP. The plankton extract can effectively protect cells against negative effects caused by cold shock and heat shock, and has the effect of relieving temperature aging.
③ Desert rose leaf cell extract
The preparation method comprises the following steps: the desert rose leaf cell extract is prepared by selecting the callus of desert rose (ADENIUM OBESUM) She Shengcheng (preferably desert rose), suspending and proliferating, performing biological amplification, purifying to obtain a cell suspension, extracting, filtering and sterilizing.
Efficacy: desert rose is a good environment for high temperature drying and sunlight, is resistant to heat and cold, and is named as a desert rose because the original place is close to the desert and red like the rose. The desert rose leaf cell extract has good antioxidation effect, can reduce the generation of free radicals, inhibit cell dysfunction caused by the free radicals, comprises skin dehydration and the like, and further proves that lipid peroxidation translated from MDA can be reduced.
④ European Chaetoceros herb extract
The preparation method comprises the following steps: the European-grass extract refers to a forsythiaside B active ingredient with the concentration of 350ppm extracted by an extraction process through selecting European-grass leaves to generate callus.
Efficacy: protecting skin cells from penetration of contaminants and genetic damage, cleaning and neutralizing toxin oxides from disrupting protein and lipid structures. Thereby helping to clean the skin, helping the skin to keep original fineness and preventing the complexion from becoming dull.
⑤ Hydrolyzed yeast protein
The preparation method comprises the following steps: the hydrolyzed yeast protein is derived from hydrolysate of plant such as single grain wheat (Triticum monococum), potato (Solanum tuberosum), corn (Zea mayz L.) or pea (Pisum sativum) or hydrolysate of yeast from Saccharomyces, and is obtained by fermentation technology. The hydrolyzed yeast protein contains peptides with molecular weight less than 6kDa by purification.
Efficacy: in 2017, the nobel physiological or medical prize acquirer elucidated the molecular mechanisms controlling circadian rhythms. The "clocked" beat of a cell comparable to a metronome is produced by a molecular oscillator involving the following clock genes: CLOCK, BMAL, PER1, CRY. Experiments of human keratinocytes and human fibroblasts show that the hydrolyzed yeast protein effectively improves CLOCK, BMAL, PER gene expression, simultaneously increases collagen III expression, has good cell synchronization, and effectively solves the skin problems caused by social time difference and insufficient sleep.
In the composition for improving exogenous aging, through the difference of action mechanisms among the components, various components interact with each other, specifically, the sparassis crispa extract is mainly used for photo-aging, the plankton extract is used for cold-hot aging, the rose leaf cell extract in desert is used for oxidative aging, the European-grass extract is used for aging caused by pollution, and hydrolyzed yeast protein is used for aging caused by life modes such as irregular work and rest. The five active substances complement each other in action mechanism, the action targets fully cover common exogenous aging factors in life, and the combined application has the functions of resisting ultraviolet rays, resisting cold and hot aging, resisting oxidization, resisting pollution, regulating skin rhythm and the like, so that the effects of improving skin moisture content, brightening skin color, reducing wrinkles, shrinking pores and improving skin texture are achieved, and the functions of resisting aging are combined. In addition, glycerin can be used as a humectant, and the finally prepared composition for improving exogenous aging has the effects of relieving the influence of environment and bad living habit on skin, improving the moisture content of the skin, brightening skin color, reducing wrinkles, shrinking pores and improving skin texture.
In the following examples, the unit of parts by weight may be g or kg.
Example 1
The embodiment provides an exogenous aging improving emulsion, which comprises the following raw material components:
10% of an exogenous aging improving composition, 0.2% of xanthan gum, 3% of 1, 3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1, 2-hexanediol and the balance of deionized water.
Wherein the raw material components of the composition comprise, by weight based on the total weight of the exogenous senescence improving composition:
10 parts of sparassis crispa extract, 40 parts of plankton extract, 20 parts of desert rose leaf cell extract, 10 parts of European decumbent corydalis herb extract and 20 parts of hydrolyzed yeast protein.
The exogenous aging improving emulsion is prepared by the following method:
Pre-dispersing 1, 3-butanediol, EDTA-2Na and xanthan gum uniformly, adding into deionized water, and stirring until the mixture is completely dissolved; adding trehalose, stirring at 300r/min to disperse uniformly, heating to 85deg.C, adding p-hydroxyacetophenone, stirring uniformly, and stopping heating after completely dissolving; cooling to 40 ℃, adding the exogenous aging improving composition, stirring uniformly glycerol and 1, 2-hexanediol, adding into a system, stirring uniformly, discharging and subpackaging to obtain the exogenous aging improving emulsion.
Example 2
The embodiment provides an exogenous aging improving emulsion, which comprises the following raw material components:
10% of an exogenous aging improving composition, 0.2% of xanthan gum, 3% of 1, 3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1, 2-hexanediol and the balance of deionized water.
Wherein the raw material components of the composition comprise, by weight based on the total weight of the exogenous senescence improving composition:
40 parts of Sparassis crispa extract, 20 parts of plankton extract, 10 parts of desert rose leaf cell extract, 20 parts of European corydalis herb extract and 10 parts of hydrolyzed yeast protein.
The exogenous aging improving emulsion is prepared by the following method:
Pre-dispersing 1, 3-butanediol, EDTA-2Na and xanthan gum uniformly, adding into deionized water, and stirring until the mixture is completely dissolved; adding trehalose, stirring at 300r/min to disperse uniformly, heating to 85deg.C, adding p-hydroxyacetophenone, stirring uniformly, and stopping heating after completely dissolving; cooling to 40 ℃, adding the exogenous aging improving composition, stirring uniformly glycerol and 1, 2-hexanediol, adding into a system, stirring uniformly, discharging and subpackaging to obtain the exogenous aging improving emulsion.
Example 3
The embodiment provides an exogenous aging improving emulsion, which comprises the following raw material components:
10% of an exogenous aging improving composition, 0.2% of xanthan gum, 3% of 1, 3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1, 2-hexanediol and the balance of deionized water.
Wherein the raw material components of the composition comprise, by weight based on the total weight of the exogenous senescence improving composition:
30 parts of sparassis crispa extract, 30 parts of plankton extract, 15 parts of desert rose leaf cell extract, 15 parts of European decumbent corydalis herb extract and 10 parts of hydrolyzed yeast protein.
The exogenous aging improving emulsion is prepared by the following method:
Pre-dispersing 1, 3-butanediol, EDTA-2Na and xanthan gum uniformly, adding into deionized water, and stirring until the mixture is completely dissolved; adding trehalose, stirring at 300r/min to disperse uniformly, heating to 85deg.C, adding p-hydroxyacetophenone, stirring uniformly, and stopping heating after completely dissolving; cooling to 40 ℃, adding the exogenous aging improving composition, stirring uniformly glycerol and 1, 2-hexanediol, adding into a system, stirring uniformly, discharging and subpackaging to obtain the exogenous aging improving emulsion.
Comparative example
The following comparative examples differ from examples 1 to 3 only in the composition of the exogenous aging-improving composition among the raw materials of the exogenous aging-improving emulsion, and the amounts of the other raw materials and the preparation methods are the same as those of example 1.
TABLE 1 composition of exogenous aging-improving compositions in comparative examples 1-6
Effect verification test example
1. Test method
1. The tester: 187 healthy females (23-45 years old, average age 34.6 years old) were randomly divided into 9 groups of 21 individuals each (1 in midway comparative example 4 and comparative example 6, and out of test, actual 20, 21 in other groups), with unlimited sexuality, and preferably people with relatively large exogenous factors in life and work; the same formulation was used for each group. The values in tables 2-6 are averages of 21 or 20 persons per group.
2. Test time: winter (one year, the air temperature is relatively low, and the air quality is relatively poor)
3. Test area: visia full face test, C-CUBE test area is the intersection point (left side) of the outer canthus and the underside of the nose wing, and moisture content test area is the intersection point (right side) of the outer canthus and the underside of the nose wing.
4. The using method comprises the following steps: the full face is used, and is smeared once after washing the face in the morning and evening every day, and the full face is used for 4 weeks continuously, so that outdoor insolation is avoided.
5. Test conditions: the subjects were rested for 30min at a temperature of 21+ -1deg.C and a relative humidity of 50+ -5% RH for quiescence.
6. Test instrument: visia7 facial photographing system (Canfield, USA), C-Cube (PIXIENCE), moisture content tester (Finland DELFIND).
7. The effect index is as follows:
Visia 7A facial image acquisition and analysis of 8 indices of skin (spots, wrinkles, texture, pores, UV spots, brown spots, red areas, purple) were performed.
Cube is a portable integrated system that performs 3D measurements by photometric stereo measurements. By collecting 2D and 3D images of the skin, sdr, sa, sz, L, a, b, erythema, ITA, melanin and other indexes are analyzed to represent improvement of smoothness, glossiness and brightness of the skin.
The moisture content tester measures the moisture content of the surface layer of the skin, and the higher the moisture content is, the better the skin is.
8. Test period: before use (D0), for 28 days (D28)
9. And (3) data processing: all results are averages of the results of 20 or 21 human subjects actually participating in the test.
2. Test results
1) Skin moisture content improvement results
Skin moisture content tests were performed on the emulsions obtained in examples 1-3 and comparative examples 1-6. The human body detection result shows that the moisture content of the skin of the cosmetic example 3 is improved by 33.55% after 28 days compared with that of the cosmetic example before use (Table 2), which shows that the composition has the effects of moisturizing and improving the moisture content of the skin in cosmetic application.
Table 2 skin moisture content improving effect for 28 days
Description of data: a positive moisture content improvement indicates an improvement in skin moisture content. And comparing examples 1-3 with comparative examples 1-6, it can be seen that examples 1,2,3 of the present invention (i.e., containing both Sparassis crispa extract, plankton extract, rosa desertification leaf cell extract, european rhizoma corydalis extract, hydrolyzed yeast protein) have better skin moisture content than the comparative examples containing only 1-4 of the five components. It can be seen that the composition for improving exogenous aging provided by the invention comprises spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein, and the combined application of the components has a synergistic effect.
2) Skin tone improvement results
The emulsions obtained in the examples and comparative examples were subjected to skin tone testing using a C-Cube (PIXIENCE) apparatus. The test area is the intersection (random side) of the outer canthus and the underside of the nose wing.
Wherein Table 3 shows the skin tone improvement results after 28 days of use for the test subjects of examples 1-3 and comparative examples 1-6.
The improvement of the skin color of the face before and after the use of the emulsion obtained in example 3 was as shown in fig. 3, and the left and right in fig. 3 refer to the improvement pattern of the skin color of the face before and after the use for 28 days, respectively, and it was found that the brightness of the skin color was increased and redness was decreased after the use for 28 days.
L *、a*、b*, ITA DEG values of the subjects before using the products of examples and comparative examples and after day 28 were measured by C-Cube, and improvement rates were calculated, as shown in Table 3.
Taking example 1 as an example, the average values of L *、a*、b*, ita° for the first 21 subjects tested were 57.919, 10.879, 11.547, 35.346, respectively.
The subjects used the example 1 emulsion for 28 days with average values of L *、a*、b*, ITA ° of 58.510, 10.219, 11.187, 36.424, respectively.
The improvement rates of L *、a*、b* and ITA degrees are +1.02%, -6.07%, -3.12%, +3.05%, respectively.
TABLE 3 skin tone improving effect on 28 days of use
Referring to table 3, the results of human body test show that the cosmetics of examples 1,2,3 and comparative examples 1 to 6 of the present invention have skin brightness L increase, red value a decrease, yellow value b decrease, brightness ITA ° increase after 28 days of application, which indicates that the cosmetics of examples 1,2,3 and comparative examples 1 to 6 of the present invention have skin color improving effect. However, examples 1,2 and 3 (i.e., containing Sparassis crispa extract, plankton extract, desert rose leaf cell extract, european rhizoma corydalis extract, hydrolyzed yeast protein) showed better skin tone lightening effect than comparative examples 1 and 2 containing four of the above components, better than comparative examples 3 and 4 containing three of the above components, and comparative example 5 containing only two of the above components showed poorer skin tone lightening effect and comparative example 6 containing only one of the above components showed the worst skin tone lightening effect. It can be seen that the composition for improving exogenous aging provided by the invention comprises spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein, and the combined application of the components has a synergistic effect.
3) Wrinkle improvement results
The emulsions obtained in the examples and comparative examples were subjected to wrinkle testing using C-Cube (PIXIENCE) and Visia facial photographing system (Canfield, USA) equipment.
Table 4 shows 28-day wrinkle improvement results for the test subjects of examples 1-3 and comparative examples 1-6.
In one subject, the improvement of facial wrinkles before and after use of the emulsion obtained in example 3 is shown in fig. 2, and the left and right in fig. 2 refer to the improvement of forehead wrinkles before use and after use for 28 days, respectively, dark green represents significant wrinkles, light green represents fine wrinkles, and it can be seen that head lifting lines are significantly reduced after use for 28 days.
The number of periocular wrinkles, the depth of external canthus wrinkles, and the facial wrinkle scores of the subjects before and after the 28 th day of use of the products of examples and comparative examples were measured by an instrument, and improvement rates were calculated, as shown in table 4.
Taking example 1 as an example, the number of periocular wrinkles, the depth of external canthus wrinkles, and the average facial wrinkle scores for the first 21 subjects were 34.198, 50.813 μm, and 21.227, respectively.
The average values of the number of periocular wrinkles, the depth of external canthus wrinkles, and facial wrinkles score after 28 days of the subject using the emulsion of example 1 were 26.938, 42.820 μm, and 15.997, respectively.
The number of wrinkles around, the depth of wrinkles at the outer corners of the eyes, and the improvement rate of facial wrinkles were-21.23%, 15.73% and 24.64%, respectively.
TABLE 4 skin wrinkle improvement effect with 28 days of use
As can be seen from the data in Table 4, the number of wrinkles around eyes and the depths of wrinkles, as well as the scores of facial wrinkles, were improved after 28 days using the cosmetics of examples 1 to 3 and comparative examples 1 to 6, indicating that the compositions of the present invention have the effect of improving wrinkles in cosmetic applications. And comparing examples 1-3 with comparative examples 1-6, it can be seen that the inventive examples 1,2,3 (i.e., containing both Sparassis crispa extract, plankton extract, rosa desertification leaf cell extract, european rhizoma corydalis extract, hydrolyzed yeast protein) have better wrinkle-improving effects than the comparative examples containing only 1-4 of the five components. It can be seen that the composition for improving exogenous aging provided by the invention comprises spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein, and the combined application of the components has a synergistic effect.
4) Pore texture improvement results
Pore and texture tests were performed on the emulsions obtained in examples and comparative examples using Visia facial photographing system (Canfield, usa) and C-Cube (PIXIENCE) equipment, the Visia test area being the full face and the C-Cube test area being the intersection (random side) of the outer canthus with the underside of the nose wing. Table 5 shows the results of 28-day pore and texture improvement for the test subjects of examples 1-3 and comparative examples 1-6.
The improvement of the texture of the face before and after the use of the emulsion obtained in example 3 on one subject is shown in fig. 1, wherein the left and right in fig. 1 refer to the improvement chart of the texture of the face before the use and after the use for 28 days, respectively, and the texture is an index for measuring the uniformity and smoothness of the skin color. Depending on the gradation of skin tone and the peaks on the skin surface, yellow indicates raised areas and blue indicates depressed areas to make a judgment (Huang Fenglan valleys), it can be seen that the skin became finer and more uniform with 28 days of use.
Pore scores, texture scores, purple scores, skin Sa values of subjects before and after the 28 th day of use of the products of examples and comparative examples were measured by an instrument, and improvement rates were calculated as shown in Table 5.
Taking example 1 as an example, the pore score, texture score, purple score, skin Sa score averages of the first 21 subjects tested were 16.961, 5.236, 16.334, 5.234 μm, respectively.
Subjects used the example 1 emulsion for 28 days with average pore scores, texture scores, violet scores, skin Sa scores of 14.093, 4.111, 13.103, 3.745 μm, respectively.
Pore score, texture score, purple score, skin Sa value improvement rate were-16.91%, -21.49%, -19.78%, and-28.45%, respectively.
Table 5 skin pores and texture effects using 28 days
As can be seen from the data in Table 5, the pores and texture were improved after 28 days of use of the cosmetics of examples 1-3 and comparative examples 1-6, indicating that the compositions of the present invention have efficacy in improving pores and texture in cosmetic applications. And comparing examples 1-3 with comparative examples 1-6, it can be seen that the efficacy of the present invention in improving pores and texture is better in examples 1,2,3 (i.e., containing both Sparassis crispa extract, plankton extract, rosa desertification leaf cell extract, european rhizoma corydalis extract, hydrolyzed yeast protein) than in the comparative examples containing only 1-4 of the five components. It can be seen that the composition for improving exogenous aging provided by the invention comprises spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein, and the combined application of the components has a synergistic effect.
5) Consumer satisfaction investigation results
Consumer satisfaction studies were collected in the form of questionnaires, and claims to be consistent in this test using a 5-point approach, namely: 1 to 5 points, 1 point is unsatisfactory, 2 point is less satisfactory, 3 point is general, 4 point is more satisfactory, and 5 point is satisfactory. Satisfaction = score ∈4 subjects +.total effective +.100%. According to literature guidelines from method sources, test results require that the tested consumer agree that a percentage greater than 50% can support the corresponding claim.
Table 6 subjects self-assessed (satisfaction) after 28 days of use
As can be seen from Table 6, the exogenous aging-improving emulsions obtained in examples 1 to 3 and comparative examples 1 to 6 of the present invention have the effects of improving skin moisture content, brightening skin color, reducing wrinkles, shrinking pores, improving skin texture, and the product is mild and does not irritate. And comparing examples 1-3 with comparative examples 1-6, it can be seen that examples 1,2, 3 of the present invention (i.e., containing both Sparassis crispa extract, plankton extract, rosa desertification leaf cell extract, european rhizoma corydalis extract, hydrolyzed yeast protein) have better effects of improving skin moisture content, lightening skin color, reducing wrinkles, shrinking pores, improving skin texture than the comparative examples containing only 1-4 of the five components. It can be seen that the composition for improving exogenous aging provided by the invention comprises spartina alterniflora extract, plankton extract, desert rose leaf cell extract, european decumbent corydalis herb extract and hydrolyzed yeast protein, and the combined application of the components has a synergistic effect. The product of the comparative example 6 only added with hydrolyzed yeast protein is mild, does not score to 100% without stimulation, but only 95%, further shows that the synergistic effect of various components is better, and the stimulation is reduced.
According to the comprehensive instrument efficacy test and consumer self-evaluation investigation, the composition for improving exogenous aging provided by the invention is specially designed for exogenous aging, and has the effects of improving skin moisture content, brightening skin color, reducing wrinkles, shrinking pores and improving skin texture.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. It will be apparent to those skilled in the art that various modifications can be readily made to these embodiments and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments, and those skilled in the art, based on the present disclosure, should make improvements and modifications without departing from the scope of the present invention.
Claims (10)
1. A composition for improving exogenous aging, comprising the following components in parts by weight: 10-40 parts of sparassis crispa extract, 10-40 parts of plankton extract, 10-40 parts of desert rose leaf cell extract, 10-20 parts of European corydalis decumbent corydalis herb extract and 10-20 parts of hydrolyzed yeast protein;
The preparation method of the sparassis crispa extract comprises the following steps: adding purified water into Sparassis crispa mycelium, shaking, mixing, shearing at high speed, extracting at high temperature, centrifuging, collecting supernatant, concentrating under reduced pressure to obtain Sparassis crispa extract containing 1000-6000ppm veratric acid, a certain amount of beta-glucan, vitamin C and vitamin E;
the preparation method of the plankton extract comprises the following steps: taking phaga polar plankton, adding purified water to obtain suspension, grinding, extracting, centrifugally separating, filtering, and performing low-temperature pasteurization to obtain plankton extract containing diguanosine tetraphosphate;
The preparation method of the desert rose leaf cell extract comprises the following steps: selecting the callus of the desert rose She Shengcheng, carrying out biological amplification after suspension proliferation, purifying to obtain a cell suspension, extracting, filtering and sterilizing to obtain the desert rose;
The preparation method of the European decumbent corydalis herb extract comprises the following steps: selecting European corydalis tuber leaves to generate callus, carrying out biological amplification after suspension proliferation, purifying to obtain cell suspension, and extracting by an extraction process to obtain an European corydalis tuber extract containing 200-600ppm of forsythiaside B active ingredient;
the hydrolyzed yeast protein is derived from hydrolysate of single wheat, potato, corn or pea or hydrolysate of yeast from Saccharomyces, and is obtained by fermentation technology, and the hydrolyzed yeast protein contains peptide with molecular weight less than 6kDa after purification.
2. The composition for improving exogenous aging according to claim 1, wherein the composition for improving exogenous aging comprises the following components in parts by weight: 10 parts of sparassis crispa extract, 40 parts of plankton extract, 20 parts of desert rose leaf cell extract, 10 parts of European decumbent corydalis herb extract and 20 parts of hydrolyzed yeast protein; or alternatively, the first and second heat exchangers may be,
The composition for improving exogenous aging comprises the following components in parts by weight: 40 parts of Sparassis crispa extract, 20 parts of plankton extract, 10 parts of desert rose leaf cell extract, 20 parts of European corydalis herb extract and 10 parts of hydrolyzed yeast protein; or alternatively, the first and second heat exchangers may be,
The composition for improving exogenous aging comprises the following components in parts by weight: 30 parts of sparassis crispa extract, 30 parts of plankton extract, 15 parts of desert rose leaf cell extract, 15 parts of European decumbent corydalis herb extract and 10 parts of hydrolyzed yeast protein.
3. A composition for improving exogenous senescence according to claim 1 or 2, characterised in that it comprises, in addition to the spartina extract, plankton extract, rose-leaf cell extract, marjoram extract, hydrolysed yeast protein, a solvent chosen from water or glycerol or a combination of both.
4. A composition for improving exogenous senescence according to claim 1 or 2, characterised in that the sparassis crispa extract contains veratric acid, β -glucan, vitamin C, vitamin E;
The plankton extract contains diguanosine tetraphosphate;
The extract of the marjoram contains forsythoside B as an active ingredient.
5. The method for preparing the composition for improving exogenous aging according to claim 1 or 2, wherein a certain amount of spartina alterniflora extract, plankton extract, rose leaf cell extract, marjoram extract and hydrolyzed yeast protein are fully mixed according to a certain proportion and uniformly stirred to prepare the composition for improving exogenous aging.
6. Use of the exogenous senescence-improving composition according to claim 1 or 2, characterized in that it is used in the preparation of a cosmetic or skin care product comprising 0.01-50% of the exogenous senescence-improving composition, based on the total weight of the cosmetic or skin care product; and 50-99.99% of cosmetic or skin care product solvent and adjuvants.
7. The use according to claim 6, wherein the auxiliary materials comprise one or more of surfactants, thickeners, conditioning agents, humectants, preservatives, chelating agents;
The solvent comprises deionized water and glycerol.
8. The use according to claim 6, wherein the cosmetic or skin care product is formulated as an anti-aging facial cleanser, an anti-aging facial cream, an anti-aging lotion, an anti-aging mask, an anti-aging eye cream or an anti-aging gel.
9. A cosmetic or skin care product for improving exogenous aging, characterized in that it comprises 0.01 to 50% of the composition for improving exogenous aging according to claim 1 or 2, based on the total weight of the cosmetic or skin care product; and 50-99.99% of cosmetic or skin care product solvent and adjuvants.
10. The cosmetic or skin care product for improving exogenous aging according to claim 9, wherein the skin care product for improving exogenous aging is an aging delaying emulsion, and the raw material components of the aging delaying emulsion comprise, by weight: 0.01-50% of composition for improving exogenous aging, 0.2% of xanthan gum, 3% of 1, 3-butanediol, 2% of glycerol, 0.2% of trehalose, 0.05% of EDTA-2Na, 0.4% of p-hydroxyacetophenone, 0.4% of 1, 2-hexanediol and the balance of deionized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211494327.XA CN115844804B (en) | 2022-11-25 | Composition for improving exogenous aging and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211494327.XA CN115844804B (en) | 2022-11-25 | Composition for improving exogenous aging and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115844804A CN115844804A (en) | 2023-03-28 |
| CN115844804B true CN115844804B (en) | 2024-07-02 |
Family
ID=
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH565556A5 (en) * | 1974-05-31 | 1975-08-29 | Aubert Maurice | Trophodynamic marine planktonic prod. cultivation - for use in e.g. skin cosmetic compsns. |
| CN110314118A (en) * | 2019-07-10 | 2019-10-11 | 湖北省吉美化妆品有限公司 | Have effects that anti-aging, the composition repaired and its preparation method and application |
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH565556A5 (en) * | 1974-05-31 | 1975-08-29 | Aubert Maurice | Trophodynamic marine planktonic prod. cultivation - for use in e.g. skin cosmetic compsns. |
| CN110314118A (en) * | 2019-07-10 | 2019-10-11 | 湖北省吉美化妆品有限公司 | Have effects that anti-aging, the composition repaired and its preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111658818B (en) | Medical repair dressing and application thereof | |
| CN106420500B (en) | A kind of composition of the extract containing Inonotus obliquus and its application | |
| CN113786362B (en) | Toning lotion composition with effects of relieving balance and protecting skin and preparation method thereof | |
| CN110279599B (en) | Whitening composition, set containing whitening composition and preparation method of set | |
| KR101926263B1 (en) | Cosmetic compositions containing fermented extract of Origanum majorana, Corchorus olitorius and Melissa officianalis | |
| CN111686069B (en) | Blue-light-resistant repair composition containing camellia extract and application thereof | |
| CN110840821A (en) | Skin care essence and preparation method thereof | |
| CN115300432A (en) | After-sun repair composition and preparation method and application thereof | |
| CN113143830A (en) | Eye cream containing anti-aging composition and preparation method thereof | |
| CN104188859B (en) | A kind of crease-resistant skin care emulsion containing natural component extract and preparation method thereof | |
| CN113081942A (en) | Multi-dimensional hydration cream containing apple stem cells | |
| CN113101256A (en) | Anti-aging repair nanoemulsion and application thereof | |
| CN115844804B (en) | Composition for improving exogenous aging and preparation method and application thereof | |
| CN116327674A (en) | Anti-wrinkle compact micro-nano essence emulsion and preparation method thereof | |
| CN113384510B (en) | Fermented composition with anti-photoaging, relieving and cooling effects and preparation method and application thereof | |
| CN115054566B (en) | Scalp aging resisting composition and preparation method thereof | |
| CN111297733A (en) | Paeonol-containing high-moisture-retention sun-screening agent and preparation method thereof | |
| CN111110624A (en) | Whitening composition | |
| CN112972314B (en) | Eye repair composition and preparation method and application thereof | |
| CN115844804A (en) | Composition for improving exogenous aging and preparation method and application thereof | |
| CN114317651A (en) | Polygonatum sibiricum polypeptide, preparation method and application thereof | |
| CN108969465B (en) | Refreshing, relaxing and isolating sun block and preparation method thereof | |
| CN111419732A (en) | Protective composition and application thereof in indoor protective cosmetics | |
| CN117243884B (en) | Composition containing fermentation active substance for improving skin barrier, preparation method and application thereof | |
| CN111450004A (en) | Anti-aging cream cosmetic and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |