CN115804279A - Coating agent for producing selenium-rich konjak and prepared coated seed - Google Patents

Coating agent for producing selenium-rich konjak and prepared coated seed Download PDF

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Publication number
CN115804279A
CN115804279A CN202211696785.1A CN202211696785A CN115804279A CN 115804279 A CN115804279 A CN 115804279A CN 202211696785 A CN202211696785 A CN 202211696785A CN 115804279 A CN115804279 A CN 115804279A
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selenium
konjak
parts
rich
coating agent
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覃剑锋
郭邦利
蔡阳光
段龙飞
陈国爱
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Ankang Academy Of Agricultural Sciences
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Ankang Academy Of Agricultural Sciences
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Abstract

The invention relates to the technical field of konjak coating agents. The invention provides a coating agent for producing selenium-rich konjak and a prepared coated seed, wherein the coating agent comprises the following raw materials in parts by weight: 25 to 30 parts of selenium fertilizer, 5 to 7 parts of plant ash, 10 to 15 parts of yellow mud, 0.08 to 0.15 part of selenium-enriched lactobacillus, 100.16 to 0.30g of microbial agent TM-, 1 to 2 parts of wood vinegar and 20 to 26 parts of water. The coating agent can provide a large amount of absorbable selenium sources for the konjac while simply, conveniently and efficiently sterilizing the konjac seeds. By adding the selenium-rich lactic acid bacteria and the microbial assistant, inorganic selenium in the selenium fertilizer can be rapidly accumulated and converted into nano selenium and organic selenium, so that konjak can be conveniently absorbed and utilized. The occurrence of the soft rot of the konjak can be effectively prevented by adding the plant ash and the wood vinegar. By the corresponding planting method, the selenium content in the konjak can be effectively improved, and the standardized and large-scale production of the selenium-rich konjak is facilitated.

Description

Coating agent for producing selenium-rich konjak and prepared coated seed
Technical Field
The invention relates to the technical field of konjak coating agents, in particular to a coating agent for producing selenium-rich konjak and a prepared coated seed.
Background
Selenium is one of the essential trace nutrient elements for human body, and has multiple physiological functions. Is praised as 'fire species of life', 'king of anticancer', 'guard of heart'. Research shows that selenium also has a certain effect on preventing and treating the novel coronavirus infection. The dietary fiber is the seventh nutrient of human body, and the perennial herb of the genus Amorphophallus in the family Araceae of the genus Amorphophallus is the economic crop most rich in dietary fiber in the world, and has wide application in food, medicine, industry and appreciation. Meanwhile, the konjak is a plant with strong selenium gathering capability, so that the development of the selenium-rich konjak and the dietary fiber and selenium dietary supplement are the optimal choice. In recent years, researchers have performed a certain amount of work on the aspects of selenium-rich konjak standards, selenium-rich konjak deep processing and the like. But the research around the selenium-rich konjak cultivation technology is less and the progress is slow.
Disclosure of Invention
The invention aims to provide a coating agent for producing selenium-rich konjak and prepared coated seeds, which are beneficial to the standardized and large-scale production of the selenium-rich konjak.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a coating agent for producing selenium-rich konjak, which comprises the following raw materials in parts by weight: 25 to 30 parts of selenium fertilizer, 5 to 7 parts of plant ash, 10 to 15 parts of yellow mud, 0.08 to 0.15 part of selenium-enriched lactobacillus, 100.16 to 0.30 part of microbial assistant TM-, 1 to 2 parts of wood vinegar and 20 to 26 parts of water.
The invention also provides a preparation method of the coating agent, which comprises the following steps: mixing selenium fertilizer, plant ash, yellow mud, selenium-rich lactobacillus, microbial agent TM-10 and wood vinegar, adding water, and stirring to obtain the coating agent.
The invention also provides a preparation method of the coating seed for producing the selenium-rich konjak, which comprises the following steps: and spraying a Xinjunan acetate aqueous solution on the seed taro, coating the seed taro with a coating agent, and drying to obtain the coated seed for producing the selenium-rich konjak.
Preferably, the preparation method of the Xinjunan acetate aqueous solution comprises the following steps: diluting 1.26% of Xinjunan acetate aqueous solution by 160-200 times with water to obtain a Xinjunan acetate aqueous solution.
Preferably, the konjak bulbs are sprayed to the surface of the konjak bulbs to be wet.
The invention also provides a coated seed.
The invention also provides a planting method of the coated seeds, which comprises the following steps:
(1) 8-12 days before sowing, mixing 85% trichloroisocyanuric acid with soil, spreading on the ground surface, and keeping the soil humidity to be more than or equal to 40% after ploughing;
(2) Sowing coated seeds, sowing high-selenium cordyceps militaris culture medium waste around the coated seeds, and then carrying out conventional management.
Preferably, in the step (1), the dosage of the 85% trichloroisocyanuric acid is 0.8-1.2 kg/mu, and the mass ratio of the 85% trichloroisocyanuric acid to the soil is 1:8 to 12.
Preferably, one coated seed is sown in each nest during sowing in the step (2), the water content of the high-selenium cordyceps militaris culture medium waste is less than 30%, and the using amount of the high-selenium cordyceps militaris culture medium waste is 30-60 g/nest.
Preferably, the high-selenium cordyceps militaris culture medium waste is a cordyceps militaris culture medium after cordyceps militaris is produced, and the cordyceps militaris culture medium is prepared from a culture solution and wheat according to the proportion of 2.5-3.5L: 2kg of mixed preparation, wherein the preparation method of the culture solution comprises the following steps: weighing raw materials according to the proportion of 8-12 parts of glucose, 8-12 parts of sucrose, 4-6 parts of peptone, 0.8-1.2 parts of monopotassium phosphate, 0.4-0.6 part of magnesium sulfate and 950-1050 parts of water, adjusting the pH value to 7.0-7.2 after mixing, adding sodium selenite according to the proportion of 190-210 mg/kg, and mixing to obtain a culture solution.
The invention provides a coating agent for producing selenium-rich konjak and a prepared coated seed, wherein the coating agent comprises the following raw materials in parts by weight: 25 to 30 portions of selenium fertilizer, 5 to 7 portions of plant ash, 10 to 15 portions of yellow mud, 0.08 to 0.15 portion of selenium-enriched lactobacillus, 100.16 to 0.30g of microbial assistant TM-, 1 to 2 portions of wood vinegar and 20 to 26 portions of water. The method can make rhizoma Amorphophalli rich in selenium grow in selenium-deficient area, and the rhizoma Amorphophalli with different varieties can reach selenium-rich level by spraying selenium fertilizer on leaf surface in growth period, and changing selenium content of coating agent can produce rhizoma Amorphophalli with different selenium content, and is suitable for standardized and large-scale production of rhizoma Amorphophalli rich in selenium.
Compared with the prior art, the method has the following beneficial effects
1. The healthy and disease-free konjak corms with the same specification are screened through seed konjak selection and seed konjak pretreatment, and a basic guarantee is provided for producing the selenium-rich konjak.
2. By adding the selenium-rich lactic acid bacteria and the microbial assistant, inorganic selenium in the selenium fertilizer can be rapidly accumulated and converted into nano selenium and organic selenium, so that konjak can be conveniently absorbed and utilized.
3. The occurrence of the soft rot of the konjak can be effectively prevented by adding the plant ash and the wood vinegar.
4. The 85% trichloroisocyanuric acid quick-acting soil treatment agent is used for treating soil before sowing, so that the quantity of pathogenic bacteria in the soil can be effectively reduced, the konjak disease is further reduced, and the production of the selenium-enriched konjak is guaranteed.
5. High-selenium cordyceps militaris culture medium waste is scattered around the konjak bulbs during sowing, so that soil organic matters are increased, and a selenium source for growing the bulbs is supplemented.
Detailed Description
The invention provides a coating agent for producing selenium-rich konjak, which comprises the following raw materials in parts by weight: 25 to 30 parts of selenium fertilizer, 5 to 7 parts of plant ash, 10 to 15 parts of yellow mud, 0.08 to 0.15 part of selenium-enriched lactobacillus, 100.16 to 0.30 part of microbial assistant TM-, 1 to 2 parts of wood vinegar and 20 to 26 parts of water.
In the invention, the selenium fertilizer is purchased from Jiangsu Tianniang agriculture science and technology limited, and the selenium content is 9500mg/kg.
In the invention, the selenium-enriched lactic acid bacteria are preferably lactobacillus acidophilus 08001, purchased from the central dairy laboratory of northeast agriculture university.
In the present invention, the microbial adjuvant TM-10 is purchased from Kyojirun technologies, inc.
In the invention, the selenium fertilizer is preferably 27-28 parts.
In the present invention, the part of the plant ash is preferably 6 parts.
In the present invention, the yellow mud is preferably 12 to 13 parts.
In the invention, the part of the selenium-enriched lactic acid bacteria is preferably 0.10-0.12 part.
In the invention, the microbial assistant TM-10 is preferably 0.2-0.25 part.
In the present invention, the amount of the pyroligneous acid is preferably 1.5 parts.
In the present invention, the part of water is preferably 23 parts.
The invention also provides a preparation method of the coating agent, which comprises the following steps: mixing selenium fertilizer, plant ash, yellow mud, selenium-rich lactobacillus, microbial agent TM-10 and wood vinegar, adding water, and stirring to obtain the coating agent.
The invention also provides a preparation method of the coating seed for producing the selenium-rich konjak, which comprises the following steps: and spraying a Xinjunan acetate aqueous solution on the seed taro, coating the seed taro with a coating agent, and drying to obtain the coated seed for producing the selenium-rich konjak.
In the invention, the variety of the seed taro is preferably Anmagic 128 or amorphophallus konjac.
In the invention, the seed taro is preferably taro penis or corm.
In the invention, the seed taro must have the typical characteristics of the variety, has no disease spots, no damage, no insect mouth and no frostbite, and is not flowering konjac.
In the invention, the preparation method of the Xinjunan acetate aqueous solution is preferably as follows: diluting 1.26% of Xinjunan acetate aqueous solution by 160-200 times with water to obtain an Xinjunan acetate aqueous solution, and further preferably diluting 1.26% of Xinjunan acetate aqueous solution by 180 times with water to obtain an Xinjunan acetate aqueous solution.
In the invention, the spraying is carried out until the surface of the konjak bulb is wet.
The invention also provides a coated seed.
The invention also provides a planting method of the coated seeds, which comprises the following steps:
(1) 8-12 days before sowing, mixing 85% trichloroisocyanuric acid with soil, uniformly spreading on the ground surface, and keeping the soil humidity to be more than or equal to 40% after ploughing;
(2) Sowing coated seeds, sowing high-selenium cordyceps militaris culture medium waste around the coated seeds, and then performing conventional management.
In the present invention, the time for scattering 85% trichloroisocyanuric acid in step (1) is preferably 10 days before sowing.
In the present invention, in the step (1), the dosage of the 85% trichloroisocyanuric acid is preferably 0.8 to 1.2 kg/acre, and more preferably 1 kg/acre, and the mass ratio of the 85% trichloroisocyanuric acid to soil is preferably 1:8 to 12, more preferably 1:10.
in the invention, in the step (2), one coated seed is preferably sown in each nest during sowing, and the water content of the high-selenium cordyceps militaris culture medium waste is preferably less than 30%, and is further preferably 25%; the dosage of the high-selenium cordyceps militaris culture medium waste is preferably 30-60 g/per litter, and more preferably 45 g/per litter.
In the invention, the high-selenium cordyceps militaris culture medium waste is preferably a cordyceps militaris culture medium after cordyceps militaris is produced, and the cordyceps militaris culture medium is preferably prepared from culture solution and wheat according to the proportion of 2.5-3.5L: 2kg, more preferably 3L of wheat: 2kg of mixed preparation
In the invention, the preparation method of the culture solution comprises the following steps: weighing raw materials according to the proportion of 8-12 parts of glucose, 8-12 parts of sucrose, 4-6 parts of peptone, 0.8-1.2 parts of monopotassium phosphate, 0.4-0.6 part of magnesium sulfate and 950-1050 parts of water, adjusting the pH value to 7.0-7.2 after mixing, adding sodium selenite according to the proportion of 190-210 mg/kg, and mixing to obtain a culture solution, further preferably weighing raw materials according to the proportion of 10 parts of glucose, 10 parts of sucrose, 5 parts of peptone, 1 part of monopotassium phosphate, 0.5 part of magnesium sulfate and 1000 parts of water, adjusting the pH value to 7.1 after mixing, adding sodium selenite according to the proportion of 200mg/kg, and mixing to obtain the culture solution.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1. Seed taro selection: the Anmagic 128 taro rhizome or 10-50 g of small seed taro is selected to be used as the seed taro for propagation, and the selected corm has the typical character of the Anmagic 128, has no scab, damage, insect bite and cold injury, and is not flowering taro.
2. Pre-treatment of seed taro: and (3) paving the konjak bulb selected in the step (1) on the ground, preparing 160-time solution of 1.26% Xinjunan acetate aqueous solution, and uniformly spraying the 160-time solution on the konjak bulb until the surface of the konjak bulb is wet.
3. Preparation of a coating agent: weighing 25kg of selenium fertilizer (produced by Jiangsu Tianniang agricultural science and technology limited company, with the selenium content of 9500 mg/kg), mixing 5kg of plant ash, 10kg of yellow mud, 80g of selenium-enriched lactobacillus-lactobacillus acidophilus 08001 (from the important dairy laboratory of northeast university of agriculture), 160g of microbial additive TM-10 (produced by Nanjing Jielian science and technology limited company) and 2000ml of wood vinegar, adding 20L of water into a 200L large container, and mechanically stirring and uniformly mixing to obtain the seed taro coating agent.
4. Coating: and (3) taking out the seed taro pretreated in the step (2), lightly putting the seed taro into a container filled with the coating agent in the step (3), and rolling until the seed ball is completely coated with the coating agent.
5. And (3) airing: and (4) taking out the coated seed balls in the step (4), and carefully placing the seeds in the sun for airing.
6. Soil treatment: at 10 days before planting, 1kg of 85% trichloroisocyanuric acid is used per mu of land, mixed with 10kg of fine soil, uniformly spread on the ground surface, immediately subjected to rotary tillage or manual soil turning, and the soil humidity is kept, so that the sterilization and disinfection effects can be achieved.
7. Sowing: during sowing, after the corms are placed, high-selenium cordyceps militaris culture medium waste (the application amount of sodium selenite is 200 mg/kg) is scattered around the corms to serve as a selenium-rich organic fertilizer raw material, and 30 g/pit is used for further supplementing a selenium source in the growing period of the corms. The management after sowing is performed according to a conventional management method of the konjak.
TABLE 1 influence of coating agent of selenium-enriched rhizoma Amorphophalli on the yield and quality of Amimor 128
Figure SMS_1
Figure SMS_2
As can be seen from table 1, after the selenium-rich konjac coating agent is treated, the whole-powder selenium content and the refined-powder selenium content of the anmagic 128 are significantly increased, so the selenium-rich konjac can be stably produced by adopting the selenium-rich konjac coating technology.
Example 2
1. Seed taro selection: selecting seed konjac with the weight of konjac of 100g to 250 g to produce commercial konjac. The selected corm must have the typical characters of the floret konjak, has no disease spots, no damage, no insect mouth and no frostbite, and is not the floret konjak.
2. Pre-treatment of seed taro: and (3) paving the konjak bulb selected in the step (1) on the ground, preparing 200 times of 1.26% Xinjunan acetate aqueous solution, and uniformly spraying the Xinjunan acetate aqueous solution on the konjak bulb until the surface of the konjak bulb is wet.
3. Preparation of a coating agent: 30kg of selenium fertilizer (produced by Jiangsu Tianniang agricultural science and technology Limited company, jiangsu, with the selenium content of 9500 mg/kg) is weighed, 7kg of plant ash, 15kg of fine yellow mud, 100g of selenium-enriched lactobacillus-lactobacillus acidophilus 08001 (from the important dairy laboratory of northeast agriculture university), 200g of microbial additive TM-10 (produced by Nanjing Jiuirun science and technology Limited company), 2000ml of wood vinegar are added, 25L of water is added, and the mixture is stirred and mixed by hand to form the seed taro coating agent.
4. Coating: and (3) taking out the seed taro pretreated in the step (2), lightly putting the seed taro into a container filled with the coating agent in the step (3), and rolling until the seed ball is completely coated with the coating agent.
5. And (3) airing: and (4) taking out the coated seed balls in the step (4), and carefully placing the seeds in a ventilated place for airing.
6. Soil treatment: at 10 days before planting, 1kg of 85% trichloroisocyanuric acid is used per mu of land, mixed with 10kg of fine soil, uniformly spread on the ground surface, immediately subjected to rotary tillage and soil turning, and the soil humidity is kept, so that the sterilization and disinfection effects can be achieved.
7. Sowing: during sowing, after the corm is placed, high-selenium cordyceps militaris culture medium waste (the application amount of sodium selenite is 200 mg/kg) is scattered around the corm to serve as a selenium-rich organic fertilizer raw material, and 60 g/pit is used for further supplementing a selenium source in the growing period of the corm.
TABLE 2 influence of selenium-rich coating agent treatment on the selenium content of each organ of amorphophallus konjac in the leaf expanding period
Figure SMS_3
As can be seen from table 2, in the leaf-spreading period of amorphophallus konjac, after the treatment with the selenium-rich konjac coating agent, the selenium content in the leaves, the petioles and the corms of amorphophallus konjac is significantly increased. Wherein the selenium content in the leaves, the petioles and the corms is that the corms are larger than the petioles and larger than the leaves in sequence, and is respectively 0.3214, 0.7110 and 1.1326mg/kg. It can be seen that the selenium in the coating agent is efficiently absorbed and utilized by the konjak under the action of the selenium-rich lactic acid bacteria.
The embodiment of the invention provides a coating agent for producing selenium-rich konjak and a prepared coated seed, wherein the coating agent comprises the following raw materials in parts by weight: 25 to 30 portions of selenium fertilizer, 5 to 7 portions of plant ash, 10 to 15 portions of yellow mud, 0.08 to 0.15 portion of selenium-enriched lactobacillus, 100.16 to 0.30g of microbial assistant TM-, 1 to 2 portions of wood vinegar and 20 to 26 portions of water. The coating agent can provide a large amount of absorbable selenium sources for the konjac while simply, conveniently and efficiently sterilizing the konjac seeds. By adding the selenium-rich lactic acid bacteria and the microbial assistant, inorganic selenium in the selenium fertilizer can be rapidly accumulated and converted into nano selenium and organic selenium, so that konjak can be conveniently absorbed and utilized. The occurrence of the soft rot of the konjak can be effectively prevented by adding the plant ash and the wood vinegar. By the corresponding planting method, the selenium content in the konjac can be effectively improved, and the standardized and large-scale production of the selenium-rich konjac is facilitated.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The coating agent for producing the selenium-rich konjak is characterized by comprising the following raw materials in parts by weight: 25 to 30 portions of selenium fertilizer, 5 to 7 portions of plant ash, 10 to 15 portions of yellow mud, 0.08 to 0.15 portion of selenium-enriched lactobacillus, 100.16 to 0.30 portion of microbial assistant TM-, 1 to 2 portions of wood vinegar and 20 to 26 portions of water.
2. The process for preparing the coating agent according to claim 1, comprising the steps of: mixing selenium fertilizer, plant ash, yellow mud, selenium-rich lactobacillus, microbial agent TM-10 and wood vinegar, adding water, and stirring to obtain the coating agent.
3. A preparation method of a coating seed for producing selenium-rich konjak is characterized by comprising the following steps: and spraying a Xinjunan acetate aqueous solution on the seed taro, coating the seed taro with a coating agent, and drying to obtain the coated seed for producing the selenium-rich konjak.
4. The preparation method according to claim 3, characterized in that the Xinjunan acetate aqueous solution is prepared by the following steps: diluting 1.26% of Xinjunan acetate aqueous solution by 160-200 times with water to obtain an Xinjunan acetate aqueous solution.
5. The preparation method according to claim 4, wherein the spraying is carried out until the surface of the konjak bulb is wet.
6. A coated seed produced by the production method according to any one of claims 3 to 5.
7. The method of growing coated seeds of claim 6, comprising the steps of:
(1) 8-12 days before sowing, mixing 85% trichloroisocyanuric acid with soil, spreading on the ground surface, and keeping the soil humidity to be more than or equal to 40% after ploughing;
(2) Sowing coated seeds, sowing high-selenium cordyceps militaris culture medium waste around the coated seeds, and then performing conventional management.
8. The planting method according to claim 7, wherein the 85% trichloroisocyanuric acid is used in the step (1) in an amount of 0.8 to 1.2 kg/acre, and the mass ratio of the 85% trichloroisocyanuric acid to soil is 1:8 to 12.
9. The planting method of claim 8, wherein one coated seed is sown in each litter during sowing in step (2), the water content of the high-selenium cordyceps militaris culture medium waste is less than 30%, and the amount of the high-selenium cordyceps militaris culture medium waste is 30-60 g/litter.
10. The planting method of any one of claims 7 to 9, wherein the high-selenium cordyceps militaris culture medium waste is a cordyceps militaris culture medium after cordyceps militaris is produced, and the cordyceps militaris culture medium is prepared from a culture solution and wheat in a volume of 2.5-3.5L: 2kg of mixed preparation, wherein the preparation method of the culture solution comprises the following steps: weighing raw materials according to the proportion of 8-12 parts of glucose, 8-12 parts of sucrose, 4-6 parts of peptone, 0.8-1.2 parts of monopotassium phosphate, 0.4-0.6 part of magnesium sulfate and 950-1050 parts of water, adjusting the pH value to 7.0-7.2 after mixing, adding sodium selenite according to the proportion of 190-210 mg/kg, and mixing to obtain a culture solution.
CN202211696785.1A 2022-12-28 2022-12-28 Coating agent for producing selenium-rich konjak and prepared coated seed Pending CN115804279A (en)

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