CN115677848A - 一种制备高纯度高稳定性蛋白质的方法 - Google Patents

一种制备高纯度高稳定性蛋白质的方法 Download PDF

Info

Publication number
CN115677848A
CN115677848A CN202211301410.0A CN202211301410A CN115677848A CN 115677848 A CN115677848 A CN 115677848A CN 202211301410 A CN202211301410 A CN 202211301410A CN 115677848 A CN115677848 A CN 115677848A
Authority
CN
China
Prior art keywords
human albumin
recombinant human
albumin
exchange chromatography
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211301410.0A
Other languages
English (en)
Chinese (zh)
Inventor
项炜
岳志蕾
董文雯
管廷臣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tonghua Anruite Biopharmaceutical Co ltd
Original Assignee
Tonghua Anruite Biopharmaceutical Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tonghua Anruite Biopharmaceutical Co ltd filed Critical Tonghua Anruite Biopharmaceutical Co ltd
Priority to CN202211301410.0A priority Critical patent/CN115677848A/zh
Publication of CN115677848A publication Critical patent/CN115677848A/zh
Priority to KR1020257013508A priority patent/KR20250088510A/ko
Priority to US19/112,951 priority patent/US20260098079A1/en
Priority to JP2025514802A priority patent/JP2025530308A/ja
Priority to EP23881442.0A priority patent/EP4610276A1/en
Priority to PCT/CN2023/115220 priority patent/WO2024087860A1/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202211301410.0A 2022-10-24 2022-10-24 一种制备高纯度高稳定性蛋白质的方法 Pending CN115677848A (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN202211301410.0A CN115677848A (zh) 2022-10-24 2022-10-24 一种制备高纯度高稳定性蛋白质的方法
KR1020257013508A KR20250088510A (ko) 2022-10-24 2023-08-28 고순도 및 고안정성 단백질을 제조하는 방법
US19/112,951 US20260098079A1 (en) 2022-10-24 2023-08-28 Method for preparing high-purity and high-stability protein
JP2025514802A JP2025530308A (ja) 2022-10-24 2023-08-28 高純度及び高安定性のタンパク質の製造方法
EP23881442.0A EP4610276A1 (en) 2022-10-24 2023-08-28 Method for preparing high-purity and high-stability protein
PCT/CN2023/115220 WO2024087860A1 (zh) 2022-10-24 2023-08-28 一种制备高纯度高稳定性蛋白质的方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211301410.0A CN115677848A (zh) 2022-10-24 2022-10-24 一种制备高纯度高稳定性蛋白质的方法

Publications (1)

Publication Number Publication Date
CN115677848A true CN115677848A (zh) 2023-02-03

Family

ID=85065784

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211301410.0A Pending CN115677848A (zh) 2022-10-24 2022-10-24 一种制备高纯度高稳定性蛋白质的方法

Country Status (6)

Country Link
US (1) US20260098079A1 (https=)
EP (1) EP4610276A1 (https=)
JP (1) JP2025530308A (https=)
KR (1) KR20250088510A (https=)
CN (1) CN115677848A (https=)
WO (1) WO2024087860A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024087860A1 (zh) * 2022-10-24 2024-05-02 通化安睿特生物制药股份有限公司 一种制备高纯度高稳定性蛋白质的方法
CN118681001A (zh) * 2024-08-19 2024-09-24 通化安睿特生物制药股份有限公司 一种含人白蛋白和葡萄糖的透析液及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5440018A (en) * 1992-05-20 1995-08-08 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
CN107428817A (zh) * 2015-03-12 2017-12-01 免疫医疗有限责任公司 纯化白蛋白融合蛋白的方法
CN112516291A (zh) * 2019-09-17 2021-03-19 通化安睿特生物制药股份有限公司 含人白蛋白的制剂及其制备方法
CN113831405A (zh) * 2021-11-17 2021-12-24 华兰生物工程重庆有限公司 一种人血白蛋白的纯化方法
WO2022120547A1 (zh) * 2020-12-08 2022-06-16 通化安睿特生物制药股份有限公司 纯化重组蛋白的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5728553A (en) * 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
CN115677848A (zh) * 2022-10-24 2023-02-03 通化安睿特生物制药股份有限公司 一种制备高纯度高稳定性蛋白质的方法
GB2636539A (en) * 2023-03-16 2025-06-25 Univ Of Sunderland Wound dressing

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5440018A (en) * 1992-05-20 1995-08-08 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
CN107428817A (zh) * 2015-03-12 2017-12-01 免疫医疗有限责任公司 纯化白蛋白融合蛋白的方法
CN112516291A (zh) * 2019-09-17 2021-03-19 通化安睿特生物制药股份有限公司 含人白蛋白的制剂及其制备方法
WO2022120547A1 (zh) * 2020-12-08 2022-06-16 通化安睿特生物制药股份有限公司 纯化重组蛋白的方法
CN113831405A (zh) * 2021-11-17 2021-12-24 华兰生物工程重庆有限公司 一种人血白蛋白的纯化方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
QING ZHANG ET AL.: "Expression and purification of recombinant human serum albumin from selectively terminable transgenic rice" *
余谦 等: "一种转基因猪血中重组人血清白蛋白分离纯化新方法" *
刘娴 等: "重组蛋白药物电荷异质性的分析技术研究进展" *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024087860A1 (zh) * 2022-10-24 2024-05-02 通化安睿特生物制药股份有限公司 一种制备高纯度高稳定性蛋白质的方法
CN118681001A (zh) * 2024-08-19 2024-09-24 通化安睿特生物制药股份有限公司 一种含人白蛋白和葡萄糖的透析液及其制备方法

Also Published As

Publication number Publication date
KR20250088510A (ko) 2025-06-17
US20260098079A1 (en) 2026-04-09
WO2024087860A1 (zh) 2024-05-02
JP2025530308A (ja) 2025-09-11
EP4610276A1 (en) 2025-09-03

Similar Documents

Publication Publication Date Title
EP2937359B1 (en) Chromatographic method for isolating and purifying high-purity recombined human serum albumin
US4342828A (en) Method for producing substance capable of stimulating differentiation and proliferation of human granulopoietic stem cells
CN105153297B (zh) 一种从Cohn组分Ⅳ沉淀中分离纯化α2-巨球蛋白的方法
EP4610276A1 (en) Method for preparing high-purity and high-stability protein
JP3438735B2 (ja) 上清ｉｖ、特にｉｖ−４またはコーンフラクションｖまたはそれと類似の上清または画分からヒトアルブミンを単離する方法
JPS63503352A (ja) タンパク質の精製
JPS58201794A (ja) ヒトインターフェロンβの濃縮精製法
US20260055389A1 (en) Method of purifying botulinum toxin
CN109929027B (zh) 采用线性洗脱步骤的重组融合蛋白纯化方法
CZ281912B6 (cs) Směs hemoglobinu síťovaného imidoesterem a způso b její výroby
CN107163138B (zh) 一种人血浆蛋白α1-抗胰蛋白酶的分离纯化方法
KR100361894B1 (ko) 치환크로마토그라피방법및정제된헤모글로빈생성물
CN103570828B (zh) 细胞培养物得到的α1蛋白酶抑制剂的纯化
JPH0479359B2 (https=)
JPH03169893A (ja) 精製方法
CA3268384A1 (en) Method for preparing high-purity and high-stability protein
DE69719382T2 (de) VERFAHREN ZUR HERSTELLUNG VON rDSPA Alpha1
Ng et al. Removal of DNA contaminants from therapeutic protein preparations
JPH02115196A (ja) エリスロポエチンの精製法
WO2021262041A1 (ru) Способ получения высокоочищенного рекомбинантного ингибитора с1-эстеразы человека
CN106977591A (zh) 一种分离纯化重组葡萄球菌蛋白a的方法
RU2799637C1 (ru) Способ получения биологически активного пептидно-аминокислотного препарата на основе плазмы крови человека
JP3476242B2 (ja) インフルエンザｈａ蛋白の精製方法
Grellet et al. An associated process for the purification of immuno globulin g, catalase, superoxide dismutase and albumin from haemolysed human placenta blood
CN111138523A (zh) 一种从重组鸡干扰素α复性液中纯化制备重组鸡干扰素α的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination