CN115607501B - Lactic acid bacteria repairing essence emulsion, emulsifying device and preparation method - Google Patents

Lactic acid bacteria repairing essence emulsion, emulsifying device and preparation method Download PDF

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CN115607501B
CN115607501B CN202211158275.9A CN202211158275A CN115607501B CN 115607501 B CN115607501 B CN 115607501B CN 202211158275 A CN202211158275 A CN 202211158275A CN 115607501 B CN115607501 B CN 115607501B
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lactobacillus
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纪宁
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Guangdong Keying Technology Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40Mixing liquids with liquids; Emulsifying
    • B01F23/41Emulsifying
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/60Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis
    • B01F27/72Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis with helices or sections of helices
    • B01F27/726Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis with helices or sections of helices with two helices with opposite pitch on the same shaft; with two helices on the same axis, driven in opposite directions or at different speeds
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The application provides a lactobacillus repair essence emulsion, an emulsifying device and a preparation method, wherein the lactobacillus repair essence emulsion comprises the following raw materials in percentage by mass: 0.5-5% of lactobacillus fermentation lysate, 0.5-5% of lactobacillus/soybean milk fermentation product filtrate, 0.1-0.5% of protein skin conditioner, 0.1-5% of plant skin conditioner, 0.1-5% of antioxidant, 5-20% of humectant, 1-10% of emollient, 1-4% of film forming agent, 1-5% of emulsifier and the balance of water. The lactobacillus repair essence emulsion disclosed by the application achieves the effects of improving the skin substrate, balancing skin water and oil, resisting sensitization and repairing the skin and strengthening the skin barrier by adding fermentation products such as lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and the like into the formula. Meanwhile, the plant skin conditioner obtained by mixing eight plant extracts is added in the formula, and the synergistic effect of the anti-allergic repair effect is realized through reasonable compatibility among the plant extracts.

Description

Lactic acid bacteria repairing essence emulsion, emulsifying device and preparation method
Technical Field
The application relates to the field of stay cable construction, in particular to lactobacillus repair essence emulsion, an emulsifying device and a preparation method.
Background
The essence emulsion is a common skin care product, is various in the current commercial essence emulsion, can be divided into whitening, moisturizing, anti-aging, anti-sensitive, acne removing and the like in terms of efficacy, but harmful substances such as heavy metal lead, mercury and the like are out of standard in many products, even forbidden components such as hormone, antibiotics and the like are added, and although the skin can be relieved in a short time, the skin is smooth and bright, but a series of problems such as red swelling, peeling, even hormonal face and the like can occur after long-term use, and the skin health is not facilitated. With the increasing use of cosmetics and other chemicals, environmental pollution, accelerated social life pace, increased pressure, and increased inclusion of sensitizers in pollen or foods, there is a growing trend in the occurrence of sensitive skin. In addition, the skin care products for partial people are often easy to cause skin allergy due to external stimulation such as improper use.
Chinese patent application No. CN201810909744.3 discloses a soothing, repairing and moisturizing essence emulsion which has effects of preventing and soothing skin sensitivity, moisturizing skin, improving skin tolerance, improving skin immunity and resistance, and promoting skin repair, but has poor effect of reconstructing skin barrier.
Disclosure of Invention
The main purpose of the application is to provide lactobacillus repair essence milk for strengthening skin barrier.
Another object of the present application is to provide an emulsifying device for preparing the above lactic acid bacteria repair essence milk.
Another object of the present application is to provide a method for preparing the above lactobacillus repair essence milk.
In order to achieve the above object, the present application provides the following technical solutions:
as a first aspect, the present application relates to a lactic acid bacteria repair essence emulsion comprising the following raw materials in mass percent:
Figure BDA0003858307970000021
the protein skin conditioner decapeptide-4 and glycoprotein comprises purslane extract, chamomile extract, centella asiatica extract, tea extract, licorice root extract, rosemary leaf extract, polygonum cuspidatum root extract and radix scutellariae root extract.
Further set up: the emulsifier comprises PEG-8C 12-20 ester, cetostearyl alcohol olive oil acid ester and sorbitan olive oil acid ester;
the lactobacillus repair essence emulsion also comprises an emulsion stabilizer, wherein the emulsion stabilizer comprises sodium polyacryl dimethyl taurate.
Further set up: the antioxidant comprises p-hydroxyacetophenone and sweet orange peel oil.
Further set up: the humectant comprises glycerin, glycerin polyether-26, trehalose, 1, 2-hexanediol and sodium hyaluronate;
the emollients include isononyl isononanoate and caprylic/capric triglyceride;
the film forming agent comprises polydimethylsiloxane and hydrogenated polyisobutene.
Further set up: the material also comprises the following raw materials in percentage by mass:
sodium benzoate 0.1-0.3%;
sodium chloride 0.1-0.5%.
As a second aspect, the present application relates to an emulsifying device for preparing the lactic acid bacteria repair essence emulsion as described above, which is characterized by comprising a frame, an emulsifying tank body, a feeding mechanism and a stirring and conveying mechanism, wherein the emulsifying tank body is barrel-shaped and is arranged along a horizontal direction, two ends of the emulsifying tank body are supported by the frame, the feeding mechanism is arranged on the upper side of the emulsifying tank body and is communicated with the emulsifying tank body, the feeding mechanism is used for mixing and inputting raw materials of each component into the emulsifying tank body, the stirring and conveying mechanism is arranged in the emulsifying tank body along the horizontal direction, the stirring and conveying mechanism comprises a stirring rotating shaft, stirring blades and a stirring motor, the stirring motor is connected with the stirring rotating shaft and is used for driving the stirring rotating shaft to rotate, the stirring blades are arranged on the stirring rotating shaft and rotate along with the rotation of the stirring rotating shaft, and the end of the emulsifying tank body, which is positioned in the stirring and conveying mechanism, is provided with a discharge port;
The stirring rotating shaft comprises a rotating outer shaft and a rotating inner shaft which are in sleeve joint fit, the stirring blades comprise a first blade and a second blade which are arranged in a staggered mode, the first blade is connected with the rotating outer shaft, a sliding groove which extends along the axial direction of the first blade is formed in the rotating outer shaft, the rotating inner shaft is connected with the second blade through the sliding groove, the rotating inner shaft and the rotating outer shaft can slide relatively along the axial direction, the second blade moves in the sliding groove along with the rotating inner shaft, and a locking mechanism used for fixing the rotating outer shaft and the rotating inner shaft is arranged between the rotating outer shaft and the rotating inner shaft;
when the second blade moves to the first end of the chute, the second blade and the first blade are spliced to form a conveying channel for conveying materials towards the tail end of the second blade; the second vane is separated from the first vane when the second vane moves to a second section of the chute.
Further set up: the locking mechanism is located at the tail end of the stirring rotating shaft and comprises a locking bolt penetrating through the rotating outer shaft, two locking threaded holes for the locking bolt to be screwed into and fixed are formed in two ends of the rotating inner shaft corresponding to the sliding grooves, a plurality of locking bolts are arranged along the circumferential direction of the rotating outer shaft, and a plurality of groups of locking threaded holes are arranged corresponding to the locking bolts.
Further set up: the feeding mechanism comprises a batching bucket communicated with the emulsifying tank body, a valve is arranged at the bottom of the batching bucket, a scraping plate which moves along the height direction in the bucket and is used for scraping ingredients attached to the inner wall of the batching bucket to the bottom of the bucket is arranged in the batching bucket, and an air cylinder used for pushing the scraping plate to move up and down is arranged at the top of the batching bucket.
As a third aspect, the present application relates to a method for preparing the lactic acid bacteria repair essence milk as described above, comprising the steps of:
the raw materials in the formula are divided into five groups according to the proportion of A phase, A1 phase, B phase, C phase and D phase, and emulsifying devices are cleaned, wherein the emulsifying devices are the emulsifying devices;
adding the A-phase raw material into an emulsifying tank body of the emulsifying device, adding the pre-dispersed A1-phase raw material into the emulsifying tank body, homogenizing, stirring, and heating to a first temperature;
adding the B-phase raw material into a feeding mechanism of the emulsifying device, heating to a first temperature to dissolve the B-phase raw material completely and discharging the B-phase raw material into an emulsifying tank body, homogenizing, stirring, preserving heat and sterilizing;
cooling the emulsifying tank body to a second temperature, adding the C-phase raw material, homogenizing and stirring, and vacuumizing and defoaming the emulsifying tank body;
Cooling the emulsifying tank body to a third temperature, adding the D-phase raw material, and stirring;
cooling the emulsifying tank body to a fourth temperature, stopping cooling, detecting physical and chemical indexes of the mixture, and discharging and storing the mixture through a discharge hole of the emulsifying tank body after the mixture is qualified;
and (5) filling, packaging and code spraying the stored mixture, and warehousing after the sampling inspection is qualified.
Further set up: the A-phase raw material comprises water and trehalose; p-hydroxyacetophenone;
the A1 phase raw material comprises sodium polyacryl dimethyl taurate, glycereth-26, sodium hyaluronate and glycerol;
the phase B raw materials comprise caprylic/capric triglyceride, polydimethylsiloxane, isononyl isononanoate, PEG-8C 12-20 acid ester, cetostearyl alcohol olive oleate, sorbitan olive oleate and hydrogenated polyisobutene;
the C-phase raw material comprises polydimethylsiloxane;
the phase D raw materials comprise water, lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate, butanediol, 1, 2-pentanediol, sweet orange peel oil, decapeptide-4, glycoprotein, herba Portulacae extract, flos Matricariae Chamomillae extract, herba Centellae extract, folium Camelliae sinensis extract, glycyrrhiza glabra root extract, herba Rosmarini officinalis leaf extract, rhizoma Polygoni Cuspidati extract and radix Scutellariae extract.
Compared with the prior art, the scheme of this application has following advantage:
1. in the lactobacillus repair essence emulsion, fermentation products such as lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and the like are added into the formula to improve the skin base, balance skin water and oil, play an anti-allergic repair role on skin, and strengthen the skin barrier. Meanwhile, the plant skin conditioner obtained by mixing eight plant extracts is added in the formula, and the synergistic effect of the anti-allergic repair effect is realized through reasonable compatibility among the plant extracts.
2. In the lactobacillus repair essence milk, lactobacillus in fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and other fermentation products can decompose plant molecules in plant extracts into small active molecules, the skin can absorb the small active molecules more quickly and effectively, the plants can be degraded usually during extraction, but the degradation is reduced in the fermentation process, the activity of the plant extracts can be enhanced, and the lactobacillus has anti-inflammatory activity, and the damage of aflatoxin to the skin can be inhibited.
3. In the emulsification device of lactic acid bacteria repair purification of this application, because the volume of emulsification tank is heavy by weight, the emulsification tank body of setting of keeping flat can make things convenient for workman clean its inside, reduces working strength. Meanwhile, the stirring and conveying mechanism of the emulsifying device can be switched into two modes so as to respectively realize the functions of transporting the mixture and stirring, and the modes of transporting the mixture are matched with the horizontally arranged emulsifying tank body, so that the mixture can be discharged smoothly; the stirring mode is to separate the whole stirring blade into a first blade and a second blade, so that the turbulence degree of fluid can be increased, and the stirring effect is improved.
Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
Drawings
The foregoing and/or additional aspects and advantages of the present application will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:
FIG. 1 is a schematic structural view of an emulsifying device for repairing and purifying lactic acid bacteria;
FIG. 2 is a schematic diagram of the internal structure of the emulsification device for repairing and purifying the Lactobacillus crispatus of the present application;
FIG. 3 is a schematic structural view of a material conveying channel formed by splicing a first blade and a second blade in the emulsification device for repairing and purifying lactic acid bacteria;
fig. 4 is a schematic structural view of separation of a first blade and a second blade in the emulsification device for repairing and purifying lactic acid bacteria.
Detailed Description
Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for the purpose of illustrating the present application and are not to be construed as limiting the present application.
The application discloses lactobacillus repair essence emulsion, which can improve the muscle bottom, balance skin water and oil and play a good repair effect on skin by adding a fermentation product and compounding plant extract.
The lactobacillus repair essence emulsion comprises the following raw materials in percentage by mass: 0.5-5% of lactobacillus fermentation lysate, 0.5-5% of lactobacillus/soybean milk fermentation product filtrate, 0.1-0.5% of protein skin conditioner, 0.1-5% of plant skin conditioner, 0.1-5% of antioxidant, 5-20% of humectant, 1-10% of emollient, 1-4% of film forming agent, 1-5% of emulsifier and the balance of water.
Specifically, the lactobacillus fermentation lysate and lactobacillus/soybean milk fermentation product filtrate and other fermentation products are added, the lactobacillus fermentation lysate comprises thalli and all active ingredients synthesized in the fermentation process, contains peptidoglycan, teichoic acid, protein, phospholipid, sterol, fatty acid, various enzymes, peptide, amino acid, nucleotide, extracellular polysaccharide and the like, has the effects of bacteriostasis, repair, sensitization, moisture preservation and the like, and polypeptide compounds with bacteriostasis effects of lactobacillus and the like contained in the lactobacillus fermentation lysate can have the effects of sedating and sterilizing the skin, can be adhered to epidermal cells to form a protective barrier for the skin, and amino acids, small peptides, polysaccharide and vitamin B contained in the lactobacillus fermentation lysate can remove cell free radicals, inhibit peroxidation of lipid, activate repair performance of the organism and inhibit activity of cell complex aminopeptidase so as to achieve the repair effect. Meanwhile, the lactobacillus fermentation lysate can restore the balance of the proportion of various mediums, is beneficial to the regulation and control of an immune system and relieves the skin inflammation. The lactobacillus/soybean milk fermentation product filtrate is a probiotic fermentation product obtained by the process of bionics fermentation of lactobacillus and bifidobacterium of 16 genus 35 strains, and has the effects of protecting, relieving, moisturizing, repairing and the like.
The application adds decapeptide-4 and glycoprotein as protein skin conditioning agents, wherein the decapeptide-4 is a messenger peptide, in particular a synthetic peptide consisting of arginine, aspartic acid, cysteine, glutamic acid, leucine, methionine and tyrosine, and can penetrate dermis to stimulate synthesis of collagen and elastin and stimulate proliferation of collagen, elastin and hyaluronic acid. The glycoprotein is an important component of skin cells and has the functions of protecting, identifying, immunizing and transmitting information, so that the glycoprotein has high moisturizing and water absorbing capacity, can activate cells, resist external radiation, promote collagen formation and delay cell cycle.
The plant skin conditioner is compounded by eight plant extracts, and specifically comprises purslane extract, chamomile extract, centella asiatica extract, tea extract, licorice root extract, rosemary leaf extract, polygonum cuspidatum root extract and radix scutellariae extract. The main active components of the purslane extract are purslane saponin and flavone, the chamomile extract contains volatile oil, flavonoid compounds, amino acid, chlorogenic acid, trace elements and the like, the centella asiatica extract mainly contains triterpenic acid, triterpenoid saponin and the like, the extract of tea is tea polyphenol, the main components of the extract of tea are multi-channel phenolic compounds existing in the tea, the main components of the extract of tea are catechin (flavanols), flavone and flavone-bis, anthocyanin and flowerin, phenolic acid and dephenolic acid and the like, the main active components of the extract of licorice root are compounds such as triterpenes, flavonoids (glabridin), polysaccharides, amino acid, alkaloids, organic acids (glycyrrhizic acid) and the like, the main components of the extract of rosemary are rosmarinol, carnosol and carnosic acid, the main components of the extract of giant knotweed rhizome are resveratrol and emodin, the main components of the extract of baical root is flavonoid compound, and the main components of the extract of the baical root is the plant skin conditioner compounded by the eight plant extracts are the compound.
In addition, lactobacillus in fermentation products such as lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and the like added in the application can decompose plant molecules in the plant extract into small active molecules, and the skin can absorb the small active molecules more rapidly and effectively. Meanwhile, plants are usually degraded during extraction, but degradation is reduced during fermentation, so that the activity of plant extracts can be enhanced, and lactobacillus has anti-inflammatory activity, so that the damage of aflatoxin to skin can be inhibited.
The antioxidant comprises the p-hydroxyacetophenone and sweet orange peel oil, the p-hydroxyacetophenone has good oxidation resistance and calming and relieving effects, and meanwhile, the p-hydroxyacetophenone can also be used as a preservative to ensure the freshness of emulsion and avoid bacterial and fungal invasion infection. The sweet orange peel oil has obvious effect of scavenging free radicals, can be used as an antioxidant, and has good proliferation effect on hyaluronic acid, so that the sweet orange peel oil is suitable for nursing dry and aged skin.
The humectant comprises glycerin, glycerin polyether-26, trehalose, 1, 2-hexanediol and sodium hyaluronate, and can play two good moisturizing roles on skin through mixing the raw materials, wherein the 1, 2-hexanediol has a good antibacterial and sterilizing effect, and the humectant can be used as a preservative after being compounded with p-hydroxyacetophenone added in an antioxidant, so that the preservative can be used without adding a traditional preservative, the preservative effect can be achieved, and meanwhile, the moisturizing and oxidation resistance of the application can be improved.
The emollient comprises isononyl isononanoate and caprylic/capric triglyceride, which belong to high-purity grease and have good spreadability so as to achieve the effect of moisturizing skin.
The film forming agent comprises polydimethylsiloxane and hydrogenated polyisobutene in percentage by mass, and the film forming agent can achieve the moisturizing effect through rapid film forming on skin.
The emulsifier comprises PEG-8C 12-20 acid ester, cetostearyl alcohol olive oil ester and sorbitan olive oil ester, and the emulsifier can emulsify the grease and active ingredients in the dispersed formula and can enable the raw materials to form emulsion.
Furthermore, the lactobacillus repair essence emulsion is also added with an emulsion stabilizer, and the application preferably adopts a poly (acryl dimethyl taurate) sodium group as the emulsion stabilizer, and is mainly used for controlling the viscosity of the emulsion.
In addition, sodium benzoate used as a preservative and sodium chloride used as a pH regulator can be added, wherein the content of sodium benzoate is 0.1-0.3% by mass, and the content of sodium chloride is 0.1-0.5% by mass. Besides the function of antiseptic, sodium benzoate can also be used for disinfection, and sodium chloride can also be used for cleaning and treating allergy.
In conclusion, the lactobacillus repair essence emulsion mainly improves the skin base by adding fermentation products such as lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and the like into the formula, balances skin water and oil, plays an anti-sensitization repair role on skin, and strengthens the skin barrier. Meanwhile, the plant skin conditioner obtained by mixing eight plant extracts is added in the formula, and the synergistic effect of the anti-allergic repair effect is realized through reasonable compatibility among the plant extracts.
In addition, please see fig. 1-4, this application still provides an emulsification device that can produce above-mentioned lactobacillus repair essence breast, emulsification device includes frame, emulsification jar body 1, feed mechanism 2 and stirring conveying mechanism 3, emulsification jar body 1 is cylindric and sets up along the horizontal direction, just the both ends of emulsification jar body 1 are passed through frame 4 supports, feed mechanism 2 locate emulsification jar body 1's upside and with emulsification jar body 1 intercommunication, feed mechanism 2 is followed emulsification jar body 1's length direction sets up the multiunit to can be used for carrying the raw materials of each component respectively in the emulsification jar body 1.
The stirring and conveying mechanism 3 is arranged in the emulsifying tank body 1 along the horizontal direction and comprises a stirring rotating shaft 31, stirring blades 32 and a stirring motor 33, the stirring motor 33 is arranged on the outer side of the emulsifying tank body 1 and is connected with the stirring rotating shaft 31 so that the stirring rotating shaft 31 rotates, the stirring rotating shaft 31 and the stirring blades 32 are arranged in the emulsifying tank body 1, and the stirring blades 32 are arranged on the stirring rotating shaft 31 and rotate along with the rotation of the stirring rotating shaft 31 so as to stir and homogenize or stir raw materials added into the emulsifying tank body 1. In addition, the emulsification tank body 1 is located the end of stirring conveying mechanism sets up discharge gate 11, discharge gate department is provided with the ooff valve that is used for switching discharge gate 11.
In the present embodiment, one end of the stirring motor 33 of the stirring and feeding mechanism 3 is regarded as the start end thereof, and one end distant from the stirring motor 33 is regarded as the end.
The stirring shaft 31 comprises a rotating outer shaft 311 and a rotating inner shaft 312 which are in sleeved fit, the stirring blades 32 comprise first blades 321 and second blades 322 which are arranged in a staggered mode, the first blades 321 are connected with the rotating outer shaft 311, and the second blades 322 are connected with the rotating inner shaft 312. Specifically, the rotating outer shaft 311 is provided with a chute 3111 extending along an axial direction thereof, and the second blade 322 is connected to the rotating inner shaft 312 through the chute 3111. Further, the rotation inner shaft 312 and the rotation outer shaft 311 are relatively slidable in the axial direction, and both ends of the rotation outer shaft 311 are limited to the emulsification tank body 1, that is, the rotation outer shaft 311 is rotatable in the emulsification tank body 1 without being displaced in the axial direction with the emulsification tank body 1. The distal end of the rotating inner shaft 312 extends to the outside of the rotating outer shaft 311 to facilitate pushing and pulling of the rotating inner shaft 312 to move axially with respect to the rotating outer shaft 311 such that the second blades 322 move within the sliding grooves 3111 of the rotating outer shaft 311.
Referring to fig. 3, when the second blade 322 moves to the first end of the chute 3111, the second blade and the first blade 321 are combined to form a feeding channel for feeding toward the end of the stirring and feeding mechanism, and the processed mixture can be fed toward the discharge port 11 at the end of the emulsifying tank body 1 under the condition of rotation, so as to complete the discharge of the mixture. When the second blade moves to the second end of the chute 3111, referring to fig. 4, the second blade is separated from the first blade 321 to form a plurality of blades for homogenizing or stirring the ingredients in the emulsifying tank body 1. When the number of stirring vanes 32 is increased, the degree of turbulence of the fluid in the emulsification tank body 1 can be increased, and a good stirring effect can be achieved.
Preferably, the sum of the angles of the single first blade 321 and the single second blade 322 is 360 ° in this embodiment, that is, a slice of one circle of the rotating blade is cut into two halves. The number of the first blades 321 and the second blades 322 of the present embodiment can cause the effect of fluid turbulence while avoiding the influence of the dense blades on the stirring efficiency.
The stirring device is characterized in that a locking mechanism 313 is arranged between the rotating outer shaft 311 and the rotating inner shaft 312, the locking mechanism 313 is positioned at the tail end of the stirring rotating shaft 31, the locking mechanism 313 comprises a locking bolt penetrating through the rotating outer shaft 311, a locking threaded hole for screwing the locking bolt into the rotating inner shaft 312 to fix the rotating outer shaft 311 and the rotating inner shaft 312 in a locking manner is formed in the rotating inner shaft 312, and two locking threaded holes are formed in the length direction of the rotating inner shaft 312 and respectively correspond to two ends of the second blade moving to the sliding groove 3111.
Further, a plurality of locking bolts are provided along the axial direction of the rotating outer shaft 311, and a plurality of groups of locking threaded holes are provided corresponding to a plurality of locking bolts. The locking stability of the rotating outer shaft 311 and the rotating inner shaft 312 can be improved by arranging a plurality of groups of locking bolts, and the second blades are prevented from shaking to influence the stirring effect when the fluid in the emulsifying tank body 1 is stirred.
The distal end of the inner rotating shaft 312 is also provided with a handle 3121 that also facilitates the pushing and pulling of the inner rotating shaft 312 by a worker.
The feeding mechanism 2 comprises a batching barrel 21 communicated with the emulsifying tank body 1, a valve 24 is arranged at the bottom of the batching barrel 21, a scraper 22 moving along the height direction in the batching barrel 21 is arranged in the batching barrel 21, and an air cylinder 23 for pushing the scraper 22 to move up and down is arranged at the top of the batching barrel 21. Through cylinder 23 propelling movement scraper blade 22 follow the direction of height of batching bucket 21 removes, can scrape the raw materials that adheres to at batching bucket 21 inner wall to the barrel head, reduces the raw materials of remaining at batching bucket 21, improves the accuracy of this application raw materials ratio.
In various embodiments, the scraping plate 22 may be a solid plate or a plurality of hollowed holes may be formed on the solid plate, and the outer peripheral shape of the scraping plate 22 may be matched with the inner peripheral shape of the dosing barrel 21.
The batching bucket 21 is used for receiving raw materials of different components, and can play a role in primarily mixing raw materials, when the raw materials in the batching bucket 21 meet proportioning requirements, the raw materials in the batching bucket 21 can be conveyed into the emulsifying tank body 1 by controlling the valve 24 to be opened according to a processing process, and the valve 24 can adopt an electronic valve to realize automatic control. In addition, in order to increase the discharging speed of the feeding assembly, the valve 24 may be replaced by a hydraulic pump, and when discharging is required, the raw materials in the corresponding dosing barrel 21 are pumped into the emulsifying tank body 1.
In addition, the emulsifying tank body 1 is further provided with a heating and cooling mechanism (not shown in fig. 2), in the embodiment, a medium layer is arranged on the periphery of the emulsifying tank body 1, water is filled in the medium layer, and water in the medium layer can be heated by an electric heating device installed in the emulsifying tank, so that the heating effect of the emulsifying tank body 1 is achieved. When the temperature needs to be reduced, the emulsification tank body 1 can be cooled in a water cooling circulation mode.
To sum up, emulsification tank body 1 of emulsification device of this application is the level setting, because emulsification tank's volume is heavy, emulsification tank body 1 of keeping flat the setting can make things convenient for workman clean its inside, reduces working strength. Meanwhile, the stirring and conveying mechanism 3 of the emulsifying device can be switched between two modes to respectively realize the functions of transporting the mixture and stirring, and the mode of transporting the mixture is matched with the horizontally arranged emulsifying tank body 1 to ensure that the mixture can be discharged smoothly; the stirring mode separates the whole stirring blade 32 into the first blade 321 and the second blade 322, so that the turbulence degree of the fluid can be increased, and the stirring effect is improved.
In addition, the application also relates to a preparation method of the lactobacillus repair essence emulsion, which adopts the emulsifying device in the application, and specifically comprises the following steps:
first, the materials involved in the formulation of the lactic acid bacterium-repairing essence emulsion were proportioned into five groups A, A and B, C, D, and the emulsifying device was cleaned.
In combination with the formula of the lactobacillus repair essence milk, the A phase raw material comprises water, trehalose and p-hydroxyacetophenone, the A1 phase raw material comprises sodium polyacryl dimethyl taurate, glycereth-26, sodium hyaluronate and glycerin, the B phase raw material comprises caprylic/capric triglyceride, polydimethylsiloxane, isononyl isononanoate, PEG-8 ester of C12-20 acid, cetostearyl oleate, sorbitan olive oleate and hydrogenated polyisobutene, the C phase raw material comprises polydimethylsiloxane, the D phase raw material comprises water, lactobacillus/soybean milk fermentation product filtrate, butanediol, 1, 2-pentanediol, sweet orange peel oil, decapeptide-4 driving motor, glycoprotein, purslane extract, chamomile flower extract, centella asiatica extract, tea extract, licorice root extract, rosemary leaf extract, giant knotweed root extract, radix scutellariae extract and lactobacillus fermentation lysate, wherein the water-soluble raw materials of the D phase raw materials comprise water, lactobacillus/soybean milk fermentation product filtrate, butanediol, 1, 2-pentanediol, sweet orange peel oil, ten peptide-4 driving motor, glycoprotein, purslane extract, chamomile root extract, tiger root extract and lactobacillus fermentation lysate, and the D phase raw material can be added. In addition, sodium benzoate and sodium chloride can be added into the D-phase raw material, the sodium benzoate is used as a preservative, the service time of the prepared lactobacillus repair essence emulsion can be prolonged, and the sodium chloride can play roles in pH adjustment and viscosity control.
According to the grouped raw materials, adding the A-phase raw materials into an emulsifying tank body 1 of the emulsifying device, adding the pre-dispersed A-phase raw materials into the emulsifying tank body 1, starting a stirring and conveying mechanism 3 in the emulsifying tank body 1 to homogenize the A-phase raw materials and the A1-phase raw materials added into the emulsifying tank body 1, wherein the homogenization time is 3-4 driving motors min, and the homogenization speed of a stirring blade 32 is 30-4 driving motors 0Hz. And then stirring the homogenized raw materials at a speed of 20-30Hz, and heating to the first temperature while stirring. The first temperature in this example is preset at 85 ℃.
B phase raw materials are added into a feeding mechanism 2 of the emulsifying device, and because the B phase raw materials contain fat, the B phase raw materials are heated to a first temperature, so that the B phase raw materials are completely dissolved in a batching barrel 21 and discharged into an emulsifying tank body 1, and then are homogenized and stirred, wherein the homogenization speed is 4 driving motors 0Hz, the homogenization time is 3min, then the mixture body added with the B phase raw materials is stirred at a speed of 20-30Hz at a reduced speed, and the temperature is kept for 20min, and then high-temperature sterilization is performed.
After the 20min heat preservation is finished, the heating and cooling mechanism of the emulsifying device is utilized to carry out water cooling circulation cooling on the emulsifying tank body 1, after the temperature is cooled to the second temperature, the C-phase raw material is added, the homogenization is started, the homogenization speed is 35Hz, the homogenization duration is 3min, and then the temperature is reduced to stir the mixture body added with the C-phase raw material at the speed of 20-30 Hz.
The second temperature in this example was preset at 75 ℃.
After stirring, vacuumizing and defoaming treatment is carried out on the emulsifying tank body 1, and after defoaming, water cooling circulation cooling is carried out on the emulsifying tank body 1 through the heating and cooling mechanism.
After the temperature is reduced to the third temperature, adding the D-phase raw material into the emulsifying tank body 1, stirring the mixture body added with the D-phase raw material at the speed of 20-30Hz, and continuously reducing the temperature of the emulsifying tank body 1 while stirring.
The third temperature in this embodiment is preset at 4 drive motor 5 deg.c.
After the temperature is reduced to the fourth temperature, the temperature reduction can be stopped, the physical and chemical indexes of the mixture are detected, and after the mixture is detected to be qualified, the mixture is discharged and stored through the discharge port 11 of the emulsifying tank body 1.
The fourth temperature in this example is preset at 38deg.C, and this temperature is determined so that the finished emulsion can be stored in hot areas and is not easily spoiled.
And (5) filling, packaging and code spraying the stored mixture, and warehousing after the sampling inspection is qualified.
It should be noted that, when the emulsifying device of the present application is used to prepare the lactobacillus repair essence emulsion, the homogenization and stirring operation involved in the preparation process of the lactobacillus repair essence emulsion is performed, the first blade 321 and the second blade 322 of the stirring blade 32 in the emulsifying device are in a separated state, and the turbulence degree of the fluid is improved by increasing the number of the blades, so as to improve the homogenization and stirring effect. When the mixture is required to be discharged out of the emulsifying tank body 1 for storage, the first blade 321 and the second blade 322 can be spliced to form a conveying channel for conveying the mixture by pushing the rotating inner shaft 312, and then the mixture is output through the discharging hole 11.
In addition, the lactobacillus repair essence emulsion disclosed in the application can be produced by utilizing the existing vacuum emulsifying machine except for the preparation of the emulsifying device disclosed in the application, for example, the vacuum emulsifying machine with the model of RHJ-500L, wherein the B-phase raw material can be heated and dissolved in an oil phase pot before being added into the emulsifying pot, and the B-phase raw material is vacuumized into the emulsifying pot after being completely dissolved.
The effect of the lactic acid bacterium-repairing essence emulsion of the present application will be further described with reference to specific examples, but the present application is not limited to the following embodiments.
Example 1
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 66.6% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
D phase raw materials: 2% of lactobacillus fermentation lysate, 2% of lactobacillus/soybean milk fermentation product filtrate, 5% of 1, 2-pentanediol, 1% of sweet orange peel oil, 40.2% of decapeptide, 0.2% of glycoprotein, 0.5% of purslane extract, 0.5% of chamomile extract, 0.5% of centella asiatica extract, 0.5% of tea extract, 0.5% of licorice root extract, 0.5% of rosemary leaf extract, 0.5% of giant knotweed root extract and 0.5% of radix scutellariae extract.
Example two
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 66.6% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 262% of glycerolyether, 0.5% of sodium hyaluronate and 2% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: lactic acid bacteria fermentation lysate 3%, lactic acid bacteria/soybean milk fermentation product filtrate 3%,1, 2-pentanediol 5%, sweet orange peel oil 1%, decapeptide-40.2%, glycoprotein 0.2%, herba Portulacae extract 0.5%, flos Matricariae Chamomillae extract 0.5%, herba Centellae extract 0.5%, folium Camelliae sinensis extract 0.5%, glycyrrhiza glabra root extract 0.5%, herba Rosmarini officinalis leaf extract 0.5%, rhizoma Polygoni Cuspidati extract 0.5%, and radix Scutellariae extract 0.5%.
Example III
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 68.3% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: 2% of lactobacillus fermentation lysate, 2% of lactobacillus/soybean milk fermentation product filtrate, 5% of 1, 2-pentanediol, 1% of sweet orange peel oil, 40.2% of decapeptide, 0.2% of glycoprotein, 0.1% of purslane extract, 0.1% of chamomile extract, 0.1% of centella asiatica extract, 0.1% of tea extract, 0.1% of licorice root extract, 0.1% of rosemary leaf extract, 0.1% of giant knotweed root extract and 0.1% of radix scutellariae extract.
Example IV
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
Phase A raw materials: 66.5% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: lactobacillus fermentation lysate 2%, lactobacillus/soybean milk fermentation product filtrate 2%,1, 2-pentanediol 5%, sweet orange peel oil 1%, decapeptide-40.2%, glycoprotein 0.2%, purslane extract 0.5%, chamomile extract 0.5%, centella asiatica extract 0.5%, tea extract 0.5%, licorice root extract 0.5%, rosemary leaf extract 0.5%, giant knotweed root extract 0.5%, radix scutellariae extract 0.5%, sodium chloride 0.1%.
Example five
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 66.5% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
A1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: 2% of lactobacillus fermentation lysate, 2% of lactobacillus/soybean milk fermentation product filtrate, 5% of 1, 2-pentanediol, 1% of sweet orange peel oil, 40.2% of decapeptide, 0.2% of glycoprotein, 0.5% of purslane extract, 0.5% of chamomile extract, 0.5% of centella asiatica extract, 0.5% of tea extract, 0.5% of licorice root extract, 0.5% of rosemary leaf extract, 0.5% of giant knotweed root extract, 0.5% of radix scutellariae extract and 0.1% of sodium benzoate.
Comparative example one
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 68.6% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
Phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: 2% of lactobacillus fermentation lysate, 5% of 1, 2-pentanediol, 1% of sweet orange peel oil, 40.2% of decapeptide, 0.2% of glycoprotein, 0.5% of purslane extract, 0.5% of chamomile extract, 0.5% of centella asiatica extract, 0.5% of tea extract, 0.5% of licorice root extract, 0.5% of rosemary leaf extract, 0.5% of polygonum cuspidatum root extract and 0.5% of radix scutellariae extract.
Comparative example two
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 68.6% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
C phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: lactobacillus/soybean milk fermentation product filtrate 2%,1, 2-pentanediol 5%, sweet orange peel oil 1%, decapeptide-40.2%, glycoprotein 0.2%, purslane extract 0.5%, chamomile extract 0.5%, centella asiatica extract 0.5%, tea extract 0.5%, licorice root extract 0.5%, rosemary leaf extract 0.5%, giant knotweed root extract 0.5%, and radix scutellariae extract 0.5%.
Comparative example three
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 71.1 percent of water, 3 percent of trehalose and 0.5 percent of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: 2% of lactobacillus fermentation lysate, 2% of lactobacillus/soybean milk fermentation product filtrate, 5% of 1, 2-pentanediol, 1% of sweet orange peel oil, 40.2% of decapeptide and 0.2% of glycoprotein.
Comparative example four
According to the processing sequence of the emulsifying device, the raw materials in the embodiment comprise the following components in percentage by mass:
phase A raw materials: 70.6% of water, 3% of trehalose and 0.5% of p-hydroxyacetophenone;
a1 phase raw materials: 0.5% of polyacryl dimethyl taurine sodium, 0.5% of glycerol polyether-263% of sodium hyaluronate and 3% of glycerol;
phase B raw materials: 2% of caprylic/capric triglyceride, 1% of polydimethylsiloxane, 2% of isononyl isononanoate, 1% of PEG-8 ester of C12-20 acid, 1% of cetostearyl-l-oleyl oleate, 1% of sorbitan-oleyl oleate and 1% of hydrogenated polyisobutene;
c phase raw materials: 1% of polydimethylsiloxane;
d phase raw materials: 1, 2-pentanediol 5%, sweet orange peel oil 1%, decapeptide-40.2%, glycoprotein 0.2%, purslane extract 0.5%, chamomile extract 0.5%, centella asiatica extract 0.5%, tea extract 0.5%, licorice root extract 0.5%, rosemary leaf extract 0.5%, giant knotweed root extract 0.5%, and radix scutellariae extract 0.5%.
The lactic acid bacteria repair essence milk obtained by the disclosed examples and comparative examples according to the preparation method disclosed above is subjected to a safety test, a repair efficacy test, a skin moisture retention test and a corrosion protection test respectively.
1. Safety test
Selecting 100 subjects according to the specification requirements of 2015 edition of cosmetic safety technical Specification by using a human skin patch test method, wherein 10 subjects are selected in one group for 10 groups; group 1 subjects were blank, no material was added to the plaque assay wells; subjects in groups 2-10 respectively carry out patch tests for a plurality of times by using the essence emulsions in examples one to five and comparative examples one to four, and a sample to be tested is placed into a testing hole of a patch tester with the dosage of 0.020g; the test operation is specifically as follows:
applying the patch tester and blank control with the test object to the same position on the forearm curved side of each group of subjects, and applying the patch tester and blank control on skin uniformly by palm light pressure for 24h; removing the spot tester for 30min, and observing skin reaction after the indentation disappears; if the result is negative, after resting for 24 hours, the plaque tester and the blank control with the test object are added for the second time at the same position for 24 hours; removing the spot tester for 30min, and observing skin reaction after the indentation disappears; if the result is negative, after resting for 24 hours, the plaque tester and the blank control with the test object are added for the third time at the same position for 24 hours; removing the spot tester for 30min, and observing skin reaction after the indentation disappears; if the result is negative, the skin reaction condition after the patch test is observed at 24 hours and 48 hours after the patch test respectively.
Wherein, the grading standard of the skin adverse reaction is as follows:
the negative reaction is grade 0; suspicious reaction: only weak erythema was grade 1; weak positive reaction: erythema, infiltration, edema, and possibly papules of grade 2; strong positive reaction: erythema, infiltration, edema, papules, herpes, responses that may be grade 3 beyond the test area; very strong positive response (fusogenic herpes response): obvious erythema, severe infiltration, edema, and blepharospermia, the response was grade 4 beyond the test area.
The experimental results are detailed in table 1:
table 1 lactic acid bacteria repair essence milk safety test number of people
Figure BDA0003858307970000181
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Figure BDA0003858307970000191
As can be seen from the detection results in Table 1, after the essence emulsion disclosed by the application is applied to a subject, no adverse reaction occurs in the 48-hour patch test results, and the fact that the tested product has no adverse reaction to the skin of a human body is judged, so that the safety reaches the standard.
2. Test of repair efficacy and test of moisture retention of skin
The skin erythema index is adopted to judge the repairing effect after the essence milk is used, the skin Erythema Index (EI) reflects the condition of facial capillaries, the inflammatory degree is visually reflected, and the lower the EI value is, the better the effects of repairing skin and improving skin erythema reaction are shown.
100 subjects were selected, 50 subjects for men and women, were randomly grouped into 10 groups, and 10 for each group were respectively corresponding to a blank control group, examples one to five, comparative examples one to four, uniform clothes, exposure of both arms, and after the same period of sun exposure for 2 hours in 2022 month 7, skin erythema index and skin moisture content were respectively tested. After sun exposure, the essence emulsions of examples one to five and comparative examples one to four are respectively smeared, the blank control group is not smeared with any skin care product, and the smearing is repeated once again at intervals of 8 hours after the smearing. Skin erythema index and skin moisture content were tested after 1h and 24h of application, respectively, and the percentage decrease in skin erythema index and the percentage increase in skin moisture content after repair were counted.
Percent (%) reduction of post-repair skin erythema index = (post-repair skin erythema index before sun-repair skin erythema index after sun)/post-repair skin erythema index before sun ×100%;
percentage of increase (%) in skin moisture after repair = (moisture after repair after sun-moisture before repair after sun)/moisture before repair after sun × 100%.
The test results are shown in Table 2 below.
TABLE 2 skin erythema index and moisture content variation over time after sun exposure
Figure BDA0003858307970000201
The results in table 2 are combined, so that the effects of repairing, moisturizing and moisturizing can be achieved when the essence emulsion is smeared on the skin after sun-drying, and the repairing effect of the essence emulsion can be improved by increasing the contents of lactobacillus fermentation lysate and lactobacillus/soybean milk fermentation product filtrate, and the repairing effect of the essence emulsion can also be improved by increasing the content of the plant skin conditioner. The lactobacillus/soybean milk fermentation product filtrate can promote skin to absorb essence milk, so that the repairing effect and the moisturizing effect are improved. The sodium chloride can improve the repairing effect of the essence milk to a certain extent, and the chloride ions can promote the absorption of the essence milk so as to have good moisturizing effect. The sodium benzoate can also improve the repairing effect of the essence emulsion.
3. Corrosion resistance test
The essence milk prepared in the first embodiment of the present application was subjected to microbiological and physicochemical tests according to cosmetic safety technical Specification (2015).
And (1) microorganism detection: colony count (cfu/g), mold and yeast count, thermotolerant coliform, staphylococcus aureus, pseudomonas aeruginosa.
The detection results are shown in the following table 3, and the detection results meet the requirements.
TABLE 3 microbial detection results
Inspection item Unit (B) Detection result Limit value
Colony count CFU/g <10 ≤1000
Total number of moulds and yeasts CFU/g <10 ≤100
Heat-resistant coliform group /g Not detected Cannot be detected
Staphylococcus aureus /g Not detected Cannot be detected
Pseudomonas aeruginosa /g Not detected Cannot be detected
And (II) physical and chemical inspection: mercury, arsenic, lead, cadmium, dioxane.
The test results are shown in Table 4 below and are satisfactory.
TABLE 4 physicochemical test results
Figure BDA0003858307970000211
Figure BDA0003858307970000221
In addition, the essence milk prepared in the first and fifth examples was subjected to preservative efficacy test by the method of USP32 (51) microorganism preservative efficacy test.
Microorganism species:
bacteria: pseudomonas aeruginosa (Pseudomonas aeruginosa) AtCC 9027, escherichia coli (Escherichia coli) AtCC 8739, staphylococcus aureus (Staphylococcus aureus) AtCC 6538.
Mould and yeast: candida albicans (Candida albicans) AtCC 10231 and aspergillus niger (Aspergillus niger) AtCC 16404.
The test results are shown in table 5,
table 5 microbial detection results of examples one and five
Figure BDA0003858307970000222
The essence milk obtained by the formula has good antibacterial and anti-corrosion effects by combining the test results, and the essence milk added with sodium benzoate has better anti-corrosion and anti-bacterial effects.
The foregoing is only a partial embodiment of the present application, and it should be noted that, for a person skilled in the art, several improvements and modifications can be made without departing from the principle of the present application, and these improvements and modifications should also be considered as the protection scope of the present application.

Claims (6)

1. The emulsifying device for preparing the lactobacillus repair essence milk is characterized by comprising the following raw materials in percentage by mass: 0.5-5% of lactobacillus fermentation lysate, 0.5-5% of lactobacillus/soybean milk fermentation product filtrate, 0.1-0.5% of protein skin conditioner, 0.1-5% of plant skin conditioner, 0.1-5% of antioxidant, 5-20% of humectant, 1-10% of emollient, 1-4% of film forming agent, 1-5% of emulsifying agent and the balance of water, wherein the protein skin conditioner comprises decapeptide-4 and glycoprotein, and the plant skin conditioner comprises purslane extract, chamomile extract, centella asiatica extract, tea extract, licorice root extract, rosemary leaf extract, polygonum cuspidatum root extract and radix scutellariae extract;
The emulsifying device comprises a frame (4), an emulsifying tank body (1), a feeding mechanism (2) and a stirring and conveying mechanism (3), wherein the emulsifying tank body (1) is barrel-shaped and is arranged along the horizontal direction, two ends of the emulsifying tank body (1) are supported by the frame (4), the feeding mechanism (2) is arranged on the upper side of the emulsifying tank body (1) and is communicated with the emulsifying tank body (1), the feeding mechanism (2) is used for mixing and inputting raw materials of components into the emulsifying tank body (1), the stirring and conveying mechanism (3) is arranged in the emulsifying tank body (1) along the horizontal direction, the stirring and conveying mechanism (3) comprises a stirring rotating shaft (31), a stirring blade (32) and a stirring motor (33), the stirring motor (33) is connected with the stirring rotating shaft (31) and is used for driving the stirring rotating shaft (31) to rotate, the stirring blade (32) is arranged on the stirring rotating shaft (31) and rotates along with the stirring rotating body (31), and the stirring mechanism (2) is provided with a discharge port (11) for discharging a discharge port (11) of the emulsifying tank body;
the stirring rotating shaft (31) comprises a rotating outer shaft (311) and a rotating inner shaft (312) which are in sleeve joint fit, the stirring blades (32) comprise a first blade (321) and a second blade (322) which are arranged in a staggered mode, the first blade (321) is connected with the rotating outer shaft (311), a chute (3111) extending along the axial direction of the rotating outer shaft (311) is formed in the rotating outer shaft (311), the rotating inner shaft (312) is connected with the second blade (322) through the chute (3111), the rotating inner shaft (312) and the rotating outer shaft (311) can slide relatively along the axial direction, the second blade (322) moves in the chute (3111) along with the rotating inner shaft (312), and a locking mechanism (313) used for fixing the rotating outer shaft (311) and the rotating inner shaft (312) is arranged between the rotating outer shaft and the rotating inner shaft (312);
When the second blade (322) moves to the first end of the chute (3111), the second blade (322) and the first blade (321) are spliced to form a conveying channel for conveying materials towards the tail end of the conveying channel; when the second blade (322) moves to the second section of the chute (3111), the second blade (322) is separated from the first blade (321); and the sum of the angles of the first blade (321) and the second blade (322) is 360 °;
the locking mechanism (313) is located at the tail end of the stirring rotating shaft (31), the locking mechanism (313) comprises locking bolts penetrating through the rotating outer shaft (311), two locking threaded holes for the locking bolts to be screwed into and fixed are formed in the rotating inner shaft (312) at two ends corresponding to the sliding grooves (3111), a plurality of locking bolts are arranged along the circumferential direction of the rotating outer shaft (311), and a plurality of groups of locking threaded holes are formed in the corresponding locking bolts.
2. The emulsifying device for preparing lactic acid bacteria repair essence emulsion according to claim 1, characterized in that the emulsifying agent comprises PEG-8C 12-20 acid ester, cetostearyl alcohol oliveoate and sorbitan oliveoate;
the lactobacillus repair essence emulsion also comprises an emulsion stabilizer, wherein the emulsion stabilizer comprises sodium polyacryl dimethyl taurate.
3. The emulsifying device for preparing lactic acid bacteria repair essence emulsion according to claim 1, characterized in that the antioxidant comprises p-hydroxyacetophenone and sweet orange peel oil.
4. The emulsifying device for preparing the lactic acid bacteria repair essence emulsion according to claim 1, wherein the humectant comprises glycerin, glycerin polyether-26, trehalose, 1, 2-hexanediol and sodium hyaluronate;
the emollients include isononyl isononanoate and caprylic/capric triglyceride;
the film forming agent comprises polydimethylsiloxane and hydrogenated polyisobutene.
5. The emulsifying device for preparing the lactic acid bacteria repair essence emulsion according to claim 1, further comprising the following raw materials in percentage by mass:
sodium benzoate 0.1-0.3%;
sodium chloride 0.1-0.5%.
6. The emulsifying device for preparing the lactobacillus repair essence emulsion according to claim 1, wherein the feeding mechanism (2) comprises a batching barrel (21) communicated with the emulsifying tank body (1), a valve (24) is arranged at the bottom of the batching barrel (21), a scraper (22) which moves along the height direction in the batching barrel (21) and is used for scraping ingredients attached to the inner wall of the batching barrel (21) to the bottom of the barrel is arranged in the batching barrel (21), and a cylinder (23) for pushing the scraper (22) to move up and down is arranged at the top of the batching barrel (21).
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