CN115568468A - Synergistic composition containing eugenol for preventing and treating papaya aphids - Google Patents
Synergistic composition containing eugenol for preventing and treating papaya aphids Download PDFInfo
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- CN115568468A CN115568468A CN202210390303.3A CN202210390303A CN115568468A CN 115568468 A CN115568468 A CN 115568468A CN 202210390303 A CN202210390303 A CN 202210390303A CN 115568468 A CN115568468 A CN 115568468A
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- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 title claims abstract description 114
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 title claims abstract description 56
- 239000005770 Eugenol Substances 0.000 title claims abstract description 56
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 title claims abstract description 56
- 229960002217 eugenol Drugs 0.000 title claims abstract description 56
- 241001124076 Aphididae Species 0.000 title claims abstract description 29
- 241000219173 Carica Species 0.000 title claims abstract description 26
- 235000009467 Carica papaya Nutrition 0.000 title claims abstract description 25
- 230000002195 synergetic effect Effects 0.000 title claims abstract description 23
- 239000000203 mixture Substances 0.000 title claims abstract description 19
- 238000013329 compounding Methods 0.000 claims abstract description 6
- 239000005906 Imidacloprid Substances 0.000 claims description 4
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 claims description 4
- 229940056881 imidacloprid Drugs 0.000 claims description 4
- 239000004593 Epoxy Substances 0.000 claims description 3
- 239000005940 Thiacloprid Substances 0.000 abstract description 23
- HOKKPVIRMVDYPB-UVTDQMKNSA-N (Z)-thiacloprid Chemical compound C1=NC(Cl)=CC=C1CN1C(=N/C#N)/SCC1 HOKKPVIRMVDYPB-UVTDQMKNSA-N 0.000 abstract description 22
- 239000003814 drug Substances 0.000 abstract description 9
- 230000004071 biological effect Effects 0.000 abstract description 8
- 239000000575 pesticide Substances 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 239000013043 chemical agent Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 3
- 230000006353 environmental stress Effects 0.000 abstract description 2
- 241001600408 Aphis gossypii Species 0.000 description 9
- 241000700605 Viruses Species 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 6
- 239000000341 volatile oil Substances 0.000 description 5
- UEQZFAGVRGWPDK-UHFFFAOYSA-N 1-[(6-chloropyridin-3-yl)methyl]-4,5-dihydroimidazol-2-amine Chemical compound C1=NC(Cl)=CC=C1CN1C(=N)NCC1 UEQZFAGVRGWPDK-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000004557 technical material Substances 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- 241000219146 Gossypium Species 0.000 description 3
- 235000009438 Gossypium Nutrition 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- WUOACPNHFRMFPN-SECBINFHSA-N (S)-(-)-alpha-terpineol Chemical compound CC1=CC[C@@H](C(C)(C)O)CC1 WUOACPNHFRMFPN-SECBINFHSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
- 102000012440 Acetylcholinesterase Human genes 0.000 description 1
- 108010022752 Acetylcholinesterase Proteins 0.000 description 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
- 241001600407 Aphis <genus> Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000721621 Myzus persicae Species 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 229940022698 acetylcholinesterase Drugs 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- OVKDFILSBMEKLT-UHFFFAOYSA-N alpha-Terpineol Natural products CC(=C)C1(O)CCC(C)=CC1 OVKDFILSBMEKLT-UHFFFAOYSA-N 0.000 description 1
- 229940088601 alpha-terpineol Drugs 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000010630 cinnamon oil Substances 0.000 description 1
- 239000010632 citronella oil Substances 0.000 description 1
- 230000009034 developmental inhibition Effects 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000009916 joint effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000004071 soot Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of pesticides, and particularly relates to a synergistic composition containing eugenol for preventing and treating papaya aphids. A synergistic composition for preventing and treating papaya aphids and containing eugenol is prepared by compounding eugenol and thiacloprid, cycolcothrin or guadipyr. The two effective components in the synergistic composition show obvious synergistic effect when compounded, can improve the control effect on papaya aphids, further reduce the application amount of pesticides, reduce pesticide residues and relieve environmental stress; meanwhile, the drug resistance risk caused by using a single chemical agent can be reduced. In addition, the synergistic composition disclosed by the invention has good biological activity on the papaya aphids, and can provide support for developing a novel chemical medicament for preventing and treating the papaya aphids.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a synergistic composition containing eugenol for preventing and treating papaya aphids.
Background
The aphid is one of the main insect vectors of papaya ringspot mosaic disease and mainly comprises green peach aphid and cotton aphid; adult and nymphs of aphids feed on the juice of tender shoot young leaves, so that new leaves shrink and twist, and honeydew discharged by the aphids can cause soot diseases; more serious, the pathogenic virus of the papaya ringspot mosaic virus can be transmitted, the papaya ringspot mosaic virus is absorbed into aphids along with juice, aphids become aphid with virus, when the aphids with virus eat healthy plants, the virus is transmitted to the healthy plants, and the loss caused by the transmitted virus is far more serious than the direct damage of the virus. Chemical agents are one of the most commonly used means for preventing and controlling papaya aphids, but long-term application of a single chemical agent is easy to cause drug resistance of pests, and simultaneously, a plurality of problems such as pesticide residue, environmental pollution and the like are caused.
Essential oil is an important secondary metabolite existing in different tissues of plants, and has the effects of fumigating, contact killing, feeding inhibition and growth and development inhibition on insects. Eugenol, chemical name is 4-allyl-2-methoxyphenol, chemical formula is C 10 H 12 O 2 Are important components of many essential oils. Four kinds of eugenol, cinnamon oil, citronella oil and alpha-terpineol are researchedThe biological activity of the plant essential oil components on the Gossypium barbarum shows that the eugenol in the four plant essential oil components has the strongest poisoning effect on the Gossypium barbarum adult aphids, and the plant essential oil components are found to have the inhibition effect on the acetylcholinesterase and the adenosine triphosphate enzyme activity of the Gossypium barbarum.
Compounding the effective components of the pesticide with different action mechanisms is an effective and rapid way for developing new products of the pesticide and preventing and treating resistant plant diseases and insect pests at present. After different pesticide active ingredients are compounded, three action types can be shown: additive action, synergistic action and antagonistic action. The inventor finds that eugenol and thiacloprid, imidacloprid or guadipyr show synergistic effect on papaya aphid when being compounded in a certain mass ratio range through a large number of indoor biological activity tests, and related compounding reports are not seen at present.
Disclosure of Invention
The invention aims to provide a synergistic composition containing eugenol for preventing and treating papaya aphids, which aims to solve the problem of long-term use of a single chemical agent.
In order to realize the purpose, the invention provides the following technical scheme:
a synergistic composition for preventing and treating papaya aphids and containing eugenol is prepared by compounding eugenol and thiacloprid, cycolcothrin or guadipyr.
Preferably, the mass ratio of the eugenol to the thiacloprid is 1-30:30-1;
preferably, the mass ratio of the eugenol to the epoxy imidacloprid is 1-15:40-1;
preferably, the mass ratio of the eugenol to the guadipyr is 1-9:9-1.
Compared with the prior art, the invention has the following beneficial effects:
(1) The two effective components in the synergistic composition show obvious synergistic effect when compounded, can improve the control effect on papaya aphids, further reduce the application amount of pesticides, reduce pesticide residues and relieve environmental stress; meanwhile, the drug resistance risk caused by using a single chemical agent can be reduced.
(2) The synergistic composition disclosed by the invention has good biological activity on the papaya aphids, and can provide support for developing a novel chemical medicament for preventing and treating the papaya aphids.
Detailed Description
In the following, the technical solutions of the present invention will be described clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples: indoor activity test during eugenol compounding
Reagent to be tested: 99% of eugenol technical material, 98% of thiacloprid technical material, 95% of epoxychonglin technical material and 96% of guadipyr technical material;
and (3) testing aphids: cotton aphids collected from papaya plants are raised in a laboratory;
test method (refer to NT/T1154.6-2006 pesticide indoor determination test criteria insecticide part 6: insecticidal activity test insect-soaking method)
1. Preparing a single-dose mother liquor from the raw medicines, setting a plurality of groups of ratios, and preparing 7 gradient mass concentrations by using a 0.1% Tween-80 solution to mix the single-dose mother liquor and each group of ratios according to an equal ratio method, wherein each mass concentration is 50mL;
2. soaking cotton aphid nymphs of the same age in the liquid medicine for 10s, sucking redundant liquid medicine by using filter paper, and transferring to normal conditions for continuous breeding; repeating each treatment for 4 times, soaking 20 insects for each time, and setting treatment without medicament as blank control;
checking the death condition of aphid after 3.24h, respectively recording the total number of aphids and the number of dead aphids, calculating the corrected death rate of each treatment, processing and analyzing the test data by DPS software, and calculating the LC of each medicament 50 And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.
P 1 = (K/N) × 100, where P 1 For mortality (%),k is the number of dead insects, and N is the total number of treated insects;
P 2 ＝(P t －P 0 )/(1－P 0 ) In which P is 2 To correct for mortality (%), P t To address mortality (%), P 0 Blank control mortality (%);
actually measured virulence index (ATI) = (standard pharmaceutical LC) 50 Test agent LC 50 )×100；
Theoretical virulence index (TTI) = a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture;
co-toxicity coefficient (CTC) = [ measured toxicity index (ATI)/theoretical toxicity index of the mixture (TTI) ] × 100;
criteria are divided according to joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, which shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect, and the experimental results are shown in tables 1-3.
TABLE 1 indoor bioactivity assay results of eugenol and thiacloprid compounded on aphis gossypii
| Name and proportion of the medicament | LC 50 (mg/L) | ATI | TTI | CTC |
| Eugenol | 0.62 | 100.00 | -- | -- |
| Thiacloprid | 4.76 | 13.03 | -- | -- |
| 1, eugenol: thiacloprid 30 | 2.42 | 25.62 | 15.83 | 161.83 |
| 1, eugenol: thiacloprid 20 | 1.35 | 45.93 | 17.17 | 267.53 |
| 1, eugenol: thiacloprid 10 | 0.63 | 98.41 | 20.93 | 470.15 |
| 1, eugenol: thiacloprid 8 | 0.52 | 119.23 | 22.69 | 525.50 |
| 1, eugenol: thiacloprid 5 | 0.77 | 80.52 | 27.52 | 292.57 |
| 1, eugenol: thiacloprid 2 | 0.35 | 177.14 | 42.02 | 421.60 |
| 1, eugenol: thiacloprid 1 | 0.43 | 144.19 | 56.51 | 255.14 |
| Eugenol 2: thiacloprid 1 | 0.67 | 92.54 | 71.01 | 130.32 |
| Eugenol 5: thiacloprid 1 | 0.54 | 114.81 | 85.50 | 134.28 |
| Eugenol 8: thiacloprid 1 | 0.23 | 269.57 | 90.34 | 298.40 |
| Eugenol 10: thiacloprid 1 | 0.18 | 344.44 | 92.09 | 374.02 |
| 20, eugenol: thiacloprid 1 | 0.36 | 172.22 | 95.86 | 179.66 |
| Eugenol 30: thiacloprid 1 | 0.44 | 140.91 | 97.19 | 144.98 |
As can be seen from Table 1, the mass ratio of eugenol to thiacloprid is 1-30: the cotoxicity coefficient within the range of 30-1 is larger than 120, which shows that the biological activity of the compound of the eugenol and the thiacloprid to the cotton aphid of the papaya is shown as synergistic action.
TABLE 2 indoor bioactivity assay results of eugenol and cycolxylidin complex formulation on Phyllostachys carinatus
As can be seen from Table 2, the mass ratio of eugenol to cycolxylidin is 1-15: the cotoxicity coefficients within the range of 40-1 are all larger than 120, which shows that the biological activity of the compound of the eugenol and the epoxy imidacloprid to the cotton aphid of the papaya is synergistic.
TABLE 3 indoor bioactivity assay results of eugenol and guadipyr compounded on aphis carinatus
| Name and proportion of the medicament | LC 50 (mg/L) | ATI | TTI | CTC |
| Eugenol | 0.62 | 100.00 | -- | -- |
| Wubichonggua | 4.86 | 12.76 | -- | -- |
| 1, eugenol: e Piao Guangdong 9 | 2.26 | 27.43 | 21.48 | 127.71 |
| 1, eugenol: e Piao Guangdong 7 | 1.81 | 34.25 | 23.66 | 144.76 |
| 1, eugenol: e Pihong guanidine 5 | 0.93 | 66.67 | 27.30 | 244.22 |
| 1, eugenol: wubichonggua 3 | 0.46 | 134.78 | 34.57 | 389.91 |
| 1, eugenol: e imidacloprid guanidine 1 | 0.57 | 108.77 | 56.38 | 192.93 |
| Eugenol 3: e imidacloprid guanidine 1 | 0.38 | 163.16 | 78.19 | 208.67 |
| Eugenol 5: e imidacloprid guanidine 1 | 0.15 | 413.33 | 85.46 | 483.66 |
| Eugenol 7: e imidacloprid guanidine 1 | 0.53 | 116.98 | 89.09 | 131.30 |
| Eugenol 9: wubichonggua 1 | 0.22 | 281.82 | 91.28 | 308.75 |
As can be seen from Table 3, the mass ratio of eugenol to guadipyr is 1-9: the cotoxicity coefficient within the range of 9-1 is larger than 120, which shows that the biological activity of the compound of the eugenol and the guadipyr shows synergistic effect on the cotton aphid of papaya after the compound.
In conclusion, the eugenol and the nicotine thiacloprid, the epoxychongoline or the guadipyr are compounded to have synergistic effect on the biological activity of the cotton aphids of the papaya, so that the control effect on the cotton aphids of the papaya can be effectively improved.
Claims (2)
1. The synergistic composition containing the eugenol for preventing and treating the papaya aphids is characterized in that the effective component of the synergistic composition is prepared by binary compounding of the eugenol and the epoxy imidacloprid.
2. The synergistic composition containing eugenol for controlling papaya aphids as claimed in claim 1, wherein the mass ratio of eugenol to cycolxylidin is 1-15:40-1.
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| CN107347903A (en) * | 2017-09-13 | 2017-11-17 | 佛山市瑞生通科技有限公司 | A kind of composition pesticide containing cycolxylidin and matrine |
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| CN113545346B (en) | 2022-04-12 |
| CN113545346A (en) | 2021-10-26 |
| CN115669657A (en) | 2023-02-03 |
| CN115568468B (en) | 2024-02-20 |
| CN115669657B (en) | 2024-02-20 |
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