CN115531275A

CN115531275A - Soothing and repairing essence and preparation method thereof

Info

Publication number: CN115531275A
Application number: CN202211382647.6A
Authority: CN
Inventors: 郑嘉铭; 曾敏
Original assignee: Guangzhou Pinhard Biotechnology Co ltd
Current assignee: Guangzhou Pinhard Biotechnology Co ltd
Priority date: 2022-11-04
Filing date: 2022-11-04
Publication date: 2022-12-30
Anticipated expiration: 2042-11-04
Also published as: CN115531275B

Abstract

The invention discloses a soothing and repairing essence which comprises a solvent, a humectant, an antioxidant, a chelating agent, a thickening agent, a skin conditioner, a yucca brevifolia root extract, a trigeminal arabica extract, a opuntia ficus indica extract and the like, wherein the content of the skin conditioner in the soothing and repairing essence is 0.60-0.64%, the content of the yucca brevifolia root extract is 0.25-0.27%, the content of the trigeminal arabica extract is 0.25-0.28%, and the content of the opuntia ficus indica extract is 0.10-0.13%. The compound of the plant extract has the effects of promoting the generation of natural skin moisturizing factors and enhancing the oxidation resistance of skin, and the soothing and repairing essence disclosed by the invention can obviously improve the skin state of sensitive muscles. The invention also provides a method for preparing the soothing and repairing essence.

Description

Soothing and repairing essence and preparation method thereof

Technical Field

The invention relates to the field of sensitive muscle repair, in particular to a soothing and repairing essence and a preparation method thereof.

Background

In a modern multi-element society, many young people have irregular daily life and rest, have high fat and oil on the diet, and cannot stay up all night during sleeping, so that water and oil imbalance of the skin is caused, and further the skin barrier is damaged. When the skin barrier is damaged, the water-locking capacity of the skin per se is reduced, the generation amount of natural moisturizing factors of the skin is reduced, the loss of skin moisture is obviously increased, so that the skin is dry, itchy and desquamated, and becomes particularly sensitive and easy to be stimulated, namely, sensitive muscles. It follows that repairing the skin barrier is particularly important for sensitive muscles.

Aiming at the repair of the skin barrier, most of skin care products on the market at present directly act on the skin through the moisturizing factors by adding the moisturizing factors into the formula, so that the hydration capability of the skin is improved, and the effects of moisturizing the skin are achieved, so that the dryness, the pruritus and the desquamation of the skin are relieved, the effect of relieving the skin is achieved, and then the purpose of repairing the sensitive skin is achieved by additionally adding the components which can improve the skin activity and increase the thickness of the horny layer into the skin care products to gradually repair the skin barrier.

The means for repairing sensitive muscles cannot improve the self-moisturizing capability of the skin from the inner source, so that the skin is in a dry, itching and desquamation state again along with the loss of skin care products. The problem of damaged skin barriers cannot be solved fundamentally due to the temporary relieving and moisturizing effect, the problem of damaged skin barriers can be solved radically due to ingredients for improving skin activity and increasing the thickness of the horny layer, but the skin barrier repair is a long-term process, so that the skin barriers can not be repaired well in a short time due to the ingredients, the skin barriers are utilized to prevent skin moisture loss, the using effects of relieving skin dryness, pruritus and desquamation are reduced, and a user cannot experience a longer-lasting skin moisturizing effect when using the skin care product, so that the skin care product for sensitive muscles has an improvement space.

Disclosure of Invention

The application aims to provide a soothing and repairing essence and a preparation method thereof. After the skin care product for sensitive muscles is researched, the fact that the yucca brevifolia root extract, the triarrhiza glabra extract and the opuntia ficus indica extract are added into the soothing and repairing essence liquid to be compounded can effectively improve the lasting moisture retention of the skin, and further, the yucca brevifolia root extract, the triarrhiza glabra extract and the opuntia ficus indica extract can promote the generation of natural moisture retention factors of the skin, and the natural moisture retention factors have beneficial effects on long-acting moisture content maintenance of the skin and repair of the skin barrier of the sensitive muscles.

The application provides a repair essence that releives adopts following technical scheme:

the soothing and repairing essence comprises the following components in percentage by mass:

the plant extract comprises yucca brevicaulis root extract, tridactyla rex extract and Opuntia ficus-indica extract.

In the technical scheme, the yucca schidigera root extract can promote the generation of interleukin-1 alpha and has the effects of diminishing inflammation and resisting allergy.

Based on the strong inhibition effect of the Sandlacrizia extract on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, the Sandlacrizia extract has the effects of diminishing inflammation and resisting allergy.

The Opuntia ficus-indica extract can effectively eliminate free radicals and inhibit histamine release, and has anti-inflammatory and anti-allergy effects.

The soothing and repairing essence lotion is added with three plant extracts, namely yucca brevifolia root extract, trillipa largehead araa extract and opuntia ficus indica extract, so that the soothing and repairing essence lotion has the effect of promoting the generation of natural skin moisturizing factors (such as pyrrolidone carboxylic acid and the like), the self hydration capacity of the skin is improved, the water content of stratum corneum is increased, the dryness, pruritus and desquamation states of the skin are relieved, long-acting moisturizing of the skin is realized from the endogenesis, the purpose of soothing sensitive muscles is achieved, the anti-inflammatory effects of the three plant extracts are matched, the purposes of repairing skin barriers and soothing the skin are achieved, and the anti-inflammatory and anti-allergy effects are better achieved.

Preferably, the mass percentage of the yucca schidigera root extract in the soothing and repairing essence is 0.25-0.27%;

the weight percentage of the sanzhireia extract in the soothing and repairing essence is 0.25-0.28%;

the mass percentage of the cactus megacephalum extract in the soothing and repairing essence is 0.10-0.13%.

According to the technical scheme, the three plant extracts are added into the soothing and repairing essence according to the mass percentage, so that the soothing and repairing essence can better promote the generation of natural moisturizing factors of the skin, the self-hydration capacity of the skin is further improved, the water content of the horny layer is increased, the dryness, the pruritus and the desquamation of the skin can be relieved, the skin can be better conditioned from the endogenesis, the long-term moisturizing of the skin can be maintained, the skin barrier can be better repaired, and the purposes of diminishing inflammation and resisting allergy can be achieved.

Preferably, the mass ratio of the yucca brevicaulis root extract, the trimeresurus alata extract and the opuntia ficus-indica extract in the soothing and repairing essence is 5:5:2.

according to the technical scheme, the three plant extracts are added into the soothing and repairing essence according to the mass ratio, so that the soothing and repairing essence can further promote the generation of natural skin moisturizing factors, better improve the skin hydration capacity, keep the water content of the skin stratum corneum in a full state all the time, relieve the skin, moisturize the skin from an endogenous long-acting mode, and gradually repair a skin barrier.

Preferably, the plant extract also comprises ampelopsis grossedentata extract and peony root bark extract.

In the technical scheme, the ampelopsis grossedentata extract has an inhibiting effect on escherichia coli and staphylococcus aureus, so that the ampelopsis grossedentata extract has the effects of diminishing inflammation and resisting allergy.

Based on the fact that paeonol and other glycoside components contained in the peony root-bark extract have anti-inflammatory effects, the peony root-bark extract has the effects of diminishing inflammation and resisting allergy.

The five plant extracts, namely the yucca brevifolia root extract, the three-finger lark extract, the large flower cactus extract, the ampelopsis grossedentata extract and the peony root bark extract, are added into the soothing repair essence, so that the soothing repair essence can more effectively improve the oxidation resistance of the skin, promote the repair of a sebum membrane and improve the dry and desquamation state of the skin, meanwhile, the repaired sebum membrane can further inhibit the moisture loss of a horny layer and improve the water locking capacity of the skin, and the hydration capacity of the skin is improved by promoting the generation of natural skin moisturizing factors, so that the more lasting moisturizing effect is realized, the skin can be better relieved, the repair speed of a skin barrier is accelerated, and the skin state of sensitive muscles is further improved.

Preferably, the weight percentage of the ampelopsis grossedentata extract in the soothing and repairing essence is 0.10-0.11%;

the peony root-bark extract accounts for 0.45-0.50% of the relieving and repairing essence.

According to the technical scheme, the ampelopsis grossedentata extract and the peony root-bark extract are added into the soothing and repairing essence according to the mass percentage, so that the moisturizing effect and the antioxidant effect of the soothing and repairing essence are further promoted, and the repairing speed of a skin barrier is higher.

Preferably, the mass ratio of the yucca brevicaulis root extract, the three-finger larry-shaped arabian extract, the cactus grandiflora extract, the ampelopsis grossedentata extract and the peony root bark extract in the soothing and repairing essence is 5:5:2:2:10.

according to the technical scheme, five plant extracts, namely the yucca brevifolia root extract, the three-finger lark-shaped red-rooted salvia root extract, the significantly dentate ampelopsis extract and the peony root bark extract, are added into the soothing repair essence according to the mass ratio, so that the oxidation resistance of the soothing repair essence is further optimized, the repair of a sebaceous membrane is promoted, the loss of moisture in a horny layer is further reduced by the repair of the sebaceous membrane, the moisture-locking capacity of the skin is further improved, the better moisturizing effect of the soothing repair essence is realized, and the repair speed of a skin barrier is higher.

Preferably, the plant extract further comprises a purslane extract.

In the technical scheme, the purslane extract can reduce the problem of dead skin caused by dry skin and has the effect of nourishing and lubricating the skin; based on the fact that a large amount of amino acids are contained in the purslane extract, the purslane extract has the efficacy of relieving skin and inhibiting pruritus; based on the components such as flavone and saponin in the purslane extractive, the purslane extractive has the effect of resisting oxidation.

The six plant extracts, namely the yucca brevifolia root extract, the tridactylia extract, the opuntia ficus-indica extract, the ampelopsis grossedentata extract, the peony root bark extract and the purslane extract, are added into the soothing repair essence, so that the soothing repair essence can more effectively accelerate the metabolism of the skin, improve the oxidation resistance of the skin, further promote the repair speed of a sebum membrane, reduce the water loss of a horny layer, achieve the effect of long-acting moisture retention and further promote the repair speed of a skin barrier. Meanwhile, the soothing and repairing essence has the obvious effect of relieving facial fine wrinkles and achieves the anti-aging effect.

Preferably, the mass percentage of the purslane extract in the soothing and repairing essence is 5.0-5.5%.

According to the technical scheme, the purslane extract is added into the soothing repair essence, and the six plant extracts, namely the yucca brevifolia root extract, the three-finger larry-shaped arabian extract, the opuntia ficus-indica extract, the ampelopsis grossedentata extract, the peony root skin extract and the purslane extract, are compounded to further promote the metabolism of the skin, promote the repairing speed of a sebaceous membrane and enhance the water locking capacity of the skin, so that the aim of efficiently repairing the barrier of the skin is fulfilled, and simultaneously, the skin can be better resisted against aging.

Preferably, the skin conditioning agent comprises sodium hyaluronate with a molecular weight of less than 10000Da, D-panthenol.

In the technical scheme, the sodium hyaluronate with the molecular weight less than 10000Da can quickly permeate the stratum corneum and can directly enter the skin to supplement cell moisture and increase the water content of epidermal cells, so that the aims of quickly supplementing the water content of the skin and relieving the problem of dry skin are fulfilled.

Based on the smaller molecular weight of D-panthenol, the D-panthenol can effectively permeate the horny layer and infiltrate the horny layer of the skin, has a strong moisturizing effect, can effectively improve the dryness of the skin, and is helpful for relieving the skin.

Through compounding of the yucca brevifolia root extract, the trillipe largehead arabia extract, the opuntia ficus extract, the sodium hyaluronate and the D-panthenol, the purpose of quickly moistening the skin by the soothing and repairing essence is achieved, so that the dryness, the pruritus and the desquamation of the skin are relieved, the long-acting moisturizing capability of the skin is enhanced, and the skin barrier can be better repaired and the skin can be better relieved.

Preferably, the mass percentage of the sodium hyaluronate with the molecular weight less than 10000Da in the soothing repair essence lotion is 0.10-0.11%;

the mass percentage of the D-panthenol in the soothing and repairing essence lotion is 0.50-0.52%.

According to the technical scheme, the sodium hyaluronate and the D-panthenol are added according to the proportion, so that the soothing and repairing essence lotion can better moisten the skin, enhance the long-acting moisturizing capability of the skin and further promote the repair of the skin barrier.

Wherein the humectant is one or more of butanediol, 1,2 hexanediol, glycerol, polyethylene glycol, sodium alginate, sodium carboxymethylcellulose, sorbitol, propylene glycol, sodium hyaluronate, maltitol, urea, chitin and collagen.

Wherein the antioxidant is one or more of p-hydroxyacetophenone, superoxide dismutase, catalase, glutathione peroxidase, vitamin C, vitamin E, coenzyme Q10, glutathione, alpha-lipoic acid and carotenoid.

Wherein the chelating agent is one or more of EDTA-2Na, EDTA-3Na, EDTA-4Na, ethylenediamine tetraacetic acid, trisodium ethylenediamine disuccinate and caprylyl hydroximic acid.

Wherein the thickener is one or more of xanthan gum, alginate, pectin, guar gum, cationic guar gum, hydroxypropyl guar gum, carrageenin, xanthan gum and sclerotium gum.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the soothing and repairing essence lotion is added with three plant extracts, namely yucca brevifolia root extract, trillipa largeflower acanthus extract and opuntia ficus-indica extract, so that the soothing and repairing essence lotion has the effect of promoting the generation of natural skin moisturizing factors (such as pyrrolidone carboxylic acid and the like), the self-hydration capacity of the skin is improved, the dry, itching and desquamation states of the skin are relieved, long-acting skin moisturizing is realized from the endogenous source, and the anti-inflammatory effects of the three plant extracts are matched, so that the aims of repairing skin barriers and soothing the skin are fulfilled, and the anti-inflammatory and anti-allergy effects are better realized.

2. The five plant extracts, namely the yucca brevifolia root extract, the trillipia largeleaf extract, the opuntia ficus-indica extract, the ampelopsis grossedentata extract and the peony root bark extract, are added into the soothing and repairing essence, so that the soothing and repairing essence can more effectively improve the oxidation resistance of the skin, promote the repair of a sebum membrane and improve the dry and desquamation state of the skin, meanwhile, the repaired sebum membrane can further inhibit the moisture loss of a stratum corneum and improve the water locking capacity of the skin, the hydration capacity of the skin is improved by promoting the generation of natural skin moisturizing factors, a more lasting moisturizing effect is realized, the skin can be better relaxed, the repairing speed of a skin barrier is accelerated, and the skin state of sensitive muscles is further improved.

3. The six plant extracts, namely the yucca schidigera extract, the trillipe arabica extract, the cactus maximowiczii extract, the ampelopsis grossedentata extract, the peony root bark extract and the purslane extract, are added into the soothing and repairing essence, so that the soothing and repairing essence can accelerate the metabolism of the skin more effectively, improve the oxidation resistance of the skin, further accelerate the repairing speed of a sebum membrane, reduce the water loss of a cuticle, achieve the effect of long-acting moisture preservation and further accelerate the repairing speed of a skin barrier. Meanwhile, the soothing and repairing essence has the obvious effect of relieving facial fine wrinkles and achieves the anti-aging effect.

4. Through compounding of the yucca brevifolia root extract, the trillipa rey A extract, the opuntia ficus-indica extract, the sodium hyaluronate and the D-panthenol, the generation of natural moisturizing factors of the skin can be promoted based on the yucca brevifolia root extract, the trillipa rey A extract and the opuntia ficus-indica extract, and the skin epidermal cell moisture can be quickly supplemented based on the sodium hyaluronate and the D-panthenol, so that the soothing and repairing essence achieves the purpose of quickly moisturizing the skin, relieves the dryness, the itching and the desquamation of the skin, enhances the long-acting moisturizing capability of the skin, and can better repair the skin barrier and relieve the skin.

Detailed Description

The present application will be described in further detail with reference to examples.

Examples 1 to 4

A soothing and repairing essence comprises the following components shown in a table 1:

among them, xanthan gum is purchased from Tesmann Nippon (inner Mongolia) Biotech Co., ltd.

Wherein sodium hyaluronate (molecular weight less than 10000 Da) is purchased from Shandong Galaxy Biotech Co., ltd.

Among them, yucca brevifolia root extract (water extraction, ratio extract 10: 1), tripartite larara extract (water extraction, ratio extract 10: 1) were purchased from greyhounds inc.

Wherein, the cactus maximowiczii extract (water extraction method, the proportion extract is 10.

EXAMPLES 1-4 the amounts of the respective components added are shown in Table 1

Table 1 examples 1-4 raw material compositions of soothing and rejuvenating essence lotions

Preparation method of soothing and repairing essence lotion of examples 1 to 4

Step 1), sequentially putting water, butanediol, p-hydroxyacetophenone, EDTA-2Na and xanthan gum into a reaction vessel, homogenizing at 2000r/min within 5min, heating to 85 ℃, and then stirring at 80r/min to cool to 35 ℃;

and 2) putting 1,2 hexanediol, sodium hyaluronate, D-panthenol, yucca brevicaulis extract, sanzhi laregar extract and Opuntia ficus-indica extract into the solution obtained in the step 2, and homogenizing at 35 ℃ at 2000r/min for 5min to obtain the soothing and repairing essence.

Example 5

A soothing and repairing essence which is different from the essence in example 4 only in that: the plant extract also contains 0.080g Ampelopsis grossedentata extract and 0.550g peony root bark extract, and the amount of water is adjusted to 85.464g correspondingly.

When the ampelopsis grossedentata extract and the peony root bark extract are prepared into the soothing and repairing essence, 1,2 hexanediol, sodium hyaluronate (the molecular weight is less than 10000 Da), D-panthenol, a yucca brevicaulis root extract, a Sanzhi largeflower A extract and a Cactus macrocarpa extract are added into the solution obtained in the step 2 in the step 3.

Wherein the Ampelopsis grossedentata extract (water extraction, 10.

The peony root-bark extract (a peony root-bark proportion extract of 10.

Example 6

A soothing and repairing essence which is different from the essence in example 5 only in that: the plant extract also contains Ampelopsis grossedentata extract 0.120g and peony root bark extract 0.450g, and water is added in an amount of 85.524g.

Example 7

A soothing and repairing essence which is different from the essence in example 5 only in that: 0.100g of ampelopsis grossedentata extract and 0.500g of peony root and bark extract are also added into the plant extract, and correspondingly, the addition amount of water is adjusted to 85.494g.

Example 8

A soothing and repairing essence which is different from the essence in example 7 only in that: 4.500g of purslane extract is also added into the plant extract, and correspondingly, the addition amount of water is adjusted to 80.994g.

When the purslane extract is prepared into the soothing and repairing essence, 1,2 hexanediol, sodium hyaluronate (the molecular weight is less than 10000 Da), D-panthenol, yucca brevicaulis root extract, trimeresura extract, opuntia ficus-indica extract, ampelopsis grossedentata extract and peony root bark extract are added into the solution obtained in the step 2 in the step 3.

Wherein, the purslane extract (water extraction, proportion extract is 10.

Example 9

A soothing and repairing essence which is different from the essence in example 8 only in that: 5.500g of purslane extract is also added into the plant extract, and the addition amount of water is adjusted to 79.994g correspondingly.

Example 10

A soothing and repairing essence which is different from the essence in example 8 only in that: 5.000g of purslane extract is also added into the plant extract, and correspondingly, the addition amount of water is adjusted to 80.494g.

Comparative example 1

A soothing and repairing essence is different from the essence in example 4 only in that: the yucca schidigera root extract in the plant extract is replaced by evening primrose root extract.

Evening primrose root extract is commercially available, prepared by water extraction, 10:1, the proportion of evening primrose root.

Comparative example 2

A soothing and repairing essence is different from the essence in example 4 only in that: the extract of the three-finger lark in the plant extract is replaced by the agave stem extract in equal amount.

The agave stem extract is commercially available and prepared by water extraction, 10:1, the agave stem ratio extract.

Comparative example 3

A soothing and repairing essence which is different from the essence in example 4 only in that: the Opuntia ficus-indica extract in the plant extract is replaced by the Opuntia ficus-indica flower extract in equal amount.

The nopal flower extract is obtained from the market by water extraction, and is a proportional extract of nopal.

Comparative example 4

A soothing and repairing essence is different from the essence in example 7 only in that: the Ampelopsis grossedentata extract in the plant extract is replaced by the peony root bark extract in equal amount.

Comparative example 5

A soothing and repairing essence which is different from the essence in example 7 only in that: the peony root bark extract in the plant extract is replaced by the ampelopsis grossedentata extract in equal amount.

Comparative example 6

A soothing and repairing essence which is different from the essence in example 10 only in that: the purslane extract is replaced by the yucca brevifolia root extract, the opuntia ficus-indica extract, the ampelopsis grossedentata extract and the peony root bark extract in the plant extracts in equal amount.

Comparative example 7

A soothing and repairing essence which is different from the essence in example 4 only in that: sodium hyaluronate (molecular weight less than 10000 Da) was replaced with D-panthenol in equal amounts.

Comparative example 8

A soothing and repairing essence which is different from the essence in example 4 only in that: d-panthenol was replaced with sodium hyaluronate (molecular weight less than 10000 Da) in equal amounts.

Experiment 1

The content of pyrrolidone carboxylic acid in skin is determined by High Performance Liquid Chromatography (HPLC). Natural Moisturizing Factor (NMF) is mainly composed of free amino acids and their metabolites (such as pyrrolidone carboxylic acid), and thus the production of natural moisturizing factor in skin can be evaluated by measuring the change in the content of pyrrolidone carboxylic acid in skin.

The test personnel: all panelists were sensitive muscles of several dry, pruritic, desquamated, 40-45 year old, disease-free women.

Experimental reagent: sodium pyrrolidone carboxylate (purity is more than or equal to 98 percent), methanol (chromatographic purity), potassium dihydrogen phosphate (analytical purity), sodium alkane sulfonate (chromatographic purity) and phosphoric acid (chromatographic purity).

Experimental equipment: LC310 high performance liquid chromatograph (purchased from Jiangsu Tianrui instruments, inc.), analytical balance (sensory quality is 0.0001G), analytical balance (sensory quality is 0.00001G), and desktop high-speed centrifuge AXTG16G (purchased from Shanghai Zhao laboratory centrifuge, inc.).

Manufacturing a skin patch: the test persons were divided into 18 groups of 5 persons each. Each group of testers respectively uses the soothing repair essence in each example and each comparative example in the morning and evening. The application method comprises cleaning face, applying the soothing and repairing essence on the face, and beating the face with hands gently for 2min for 28 days.

Skin patch sampling was performed by the same staff operation to ensure minimal variation. The sampling is divided into two times, wherein the sampling is carried out once before the soothing repair essence is used, and the sampling is carried out once after the soothing repair essence is used for 28 days. During sampling, the D-Squame is attached to the forehead of the tester, the skin is pressed for 10-12 s at a constant Pressure by a special pressing device D-Squame Pressure Instrument, and the skin is transferred to-20 ℃ for storage and is ready for use.

Preparation of experimental sample solutions: respectively placing the skin patches in 2ml centrifuge tubes with plugs, adding 0.02mol/L potassium dihydrogen phosphate solution (containing sodium heptanesulfonate 5mmol/L, and adjusting pH to 2.5 with phosphoric acid) 1.0ml with adhesive facing inwards, performing ultrasonic treatment for 30min, centrifuging, and taking supernatant as experimental sample solution.

Preparation of experimental control solutions: accurately weighing 50mg of sodium pyrrolidone carboxylate, placing the sodium pyrrolidone carboxylate into a 10ml measuring flask, dissolving the sodium pyrrolidone carboxylate with methanol and diluting the sodium pyrrolidone carboxylate to scale, shaking the measuring flask uniformly to obtain a first mixed solution, accurately weighing 1ml of the first mixed solution from the measuring flask, placing the first mixed solution into a 100ml measuring flask, diluting the first mixed solution to scale with 0.02mol/L potassium dihydrogen phosphate solution (containing 5mmol/L sodium heptane sulfonate and regulating the pH value to 2.5 with phosphoric acid) to prepare a second mixed solution containing 50 mu g of sodium pyrrolidone carboxylate per 1ml, and diluting the second mixed solution by 10 times to obtain a control solution.

The experimental method comprises the following steps: the test sample solution and the test control solution were subjected to measurement under chromatographic conditions (column: thermo Betabasic Phenyl (250 mm. Times.4.6 mm,5 μm)), mobile phase: methanol-0.02 mol/L potassium dihydrogen phosphate solution (containing sodium heptanesulfonate 5mmol/L, phosphoric acid adjusted pH to 2.5) (3. The results are shown in Table 2.

TABLE 2 pyrrolidone Carboxylic acid content (μ g) and rate of change thereof in facial skin of test persons

Group of	Before use	After use	Content rate of change/%)
				Example 1	1.157	5.726	79.79
Example 2	1.235	6.124	79.83
				Example 3	1.193	5.908	79.80
Example 4	1.250	6.410	80.51
				Example 5	1.150	6.136	81.25
Example 6	1.186	6.564	81.93
				Example 7	1.225	6.786	81.95
Example 8	1.136	6.846	83.41
				Example 9	1.181	7.115	83.39
Example 10	1.217	7.946	84.68
				Comparative example 1	1.242	2.460	49.49
Comparative example 2	1.139	2.207	48.39
				Comparative example 3	1.210	2.411	49.81
Comparative example 4	1.228	6.387	80.77
				Comparative example 5	1.168	6.056	80.72
Comparative example 6	1.176	3.505	66.43
				Comparative example 7	1.144	4.813	76.23
Comparative example 8	1.201	5.036	76.15

The difference between the comparative examples 1-3 and the example 4 is that the combination of yucca brevifolia root extract, tiria arabica extract and opuntia ficus indica extract is not used, so that the effect of the natural moisturizing factor of the skin is not obviously influenced by the soothing and repairing essence which lacks the combination of the three plant extracts, and the soothing and repairing essence which is added with the yucca brevifolia root extract, the tiria arabica extract and the opuntia ficus indica extract can obviously enhance the generation of the natural moisturizing factor in the skin, so that the moisture content of the skin is increased from the skin endogenesis, and the effects of moisturizing the skin and soothing sensitive muscles are achieved.

Example 4 differs from example 1 in that in example 4, the mass ratio of the yucca schidigera extract, the trimeprazole alisma extract and the opuntia ficus indica extract in the soothing and repairing essence is 5:5:2, the relieving and repairing essence compounded according to the proportion is proved to be capable of further promoting the generation of natural skin moisturizing factors, and better increasing the water content of the horny layer to achieve the effect of moisturizing the skin.

Examples 5 to 7 showed no significant change in pyrrolidone carboxylic acid content compared with example 4, and thus it was confirmed that the simultaneous addition of 5 plant extracts of yucca brevicaulis root extract, trimedia extract, opuntia ficus indica extract, ampelopsis grossedentata extract, and moutan root bark extract enhanced the generation effect of natural moisturizing factors of the soothing and repairing essence to some extent.

Experiment 2

The oxidation resistance of the soothing repair essence is measured by a DPPH free radical clearance experiment.

Experimental reagent: DPPH (purity is more than or equal to 99%), absolute ethyl alcohol (analytically pure)

Experimental equipment: ultraviolet-visible spectrophotometer (available from Shanghai Jing and analytical instruments, inc.).

The experimental method comprises the following steps: the preparation concentration is 2.0 multiplied by 10 ^-4 A DPPH ethanol solution of mol/L (20 mgDPPH is weighed and added with absolute ethyl alcohol to be dissolved, and then transferred to a 250ml volumetric flask to be constant in volume); respectively weighing 1ml of the soothing repair essence to be tested, 1ml of absolute ethyl alcohol and 1ml of DPPH ethanol solution, uniformly mixing, immediately measuring the absorbance value at 517nm, calculating as A1, reacting for 30min, and thenMeasuring the absorbance value at 517nm, and recording as A2; weighing 1ml of the soothing repair essence to be detected and 1ml of absolute ethyl alcohol again, uniformly mixing, reacting for 30min, and measuring the absorbance value at 517nm, and recording as A3; DPPH clearance was calculated according to this formula: DPPH (%) = [1- (A1-A2)/A3]* The results of the measurements are shown in Table 3, at 100%.

TABLE 3 DPPH clearance assay results

Group of	DPPH clearance/%
		Example 1	78.5
Example 2	78.8
		Example 3	78.4
Example 4	79.9
		Example 5	88.0
Example 6	87.8
		Example 7	90.0
Example 8	95.9
		Example 9	96.0
Example 10	97.4
		Comparative example 1	78.4
Comparative example 2	75.5
		Comparative example 3	78.8
Comparative example 4	81.0
		Comparative example 5	82.1
Comparative example 6	83.3
		Comparative example 7	76.5
Comparative example 8	76.1

Through comparison between the example 4 and the comparative examples 1 to 3, it can be shown that the compound soothing and repairing essence containing the yucca brevifolia root extract, the trigeminal largelia extract and the opuntia ficus-indica extract enhances the free radical scavenging effect to a certain extent, and the soothing and repairing essence enhances the antioxidant effect of the skin to a certain extent.

Through comparison between the example 4 and the examples 5-7, it can be shown that the soothing and repairing essence containing 5 plant extracts of yucca brevifolia root extract, trimeresura alata extract, opuntia ficus indica extract, ampelopsis grossedentata extract and peony root bark extract has the effect of obviously improving the removal of free radicals, and the soothing and repairing essence containing 5 plant extracts can effectively improve the oxidation resistance of the skin, so that the repairing effect of a sebaceous membrane is better, and the skin barrier of sensitive muscles can be better repaired.

By comparing the example 7 with the comparative example 4 and the example 7 with the comparative example 5, it can be shown that the effect of obviously improving the clearance rate of free radicals can not be achieved by adding the ampelopsis grossedentata extract or the peony root bark extract alone, and meanwhile, by adding the 5 plant extracts of the yucca brevifolia root extract, the sanzhi largeflower acanthus extract, the ampelopsis grossedentata extract and the peony root bark extract, the clearance rate of free radicals can be obviously improved, and the relieving and repairing essence has better oxidation resistance.

Comparison of examples 8-10 with example 7 shows that the addition of six plant extracts, yucca brevifolia root extract, mirabilis javanica extract, opuntia ficus-indica extract, vitis vinifera extract, paeonia suffruticosa bark extract and purslane extract, can significantly increase the clearance of free radicals.

By comparing the example 10 with the comparative example 6, it can be shown that when the purslane extract is compounded with the yucca schidigera root extract, the trifingerlarea extract, the opuntia ficus-indica extract, the ampelopsis grossedentata extract and the peony root bark extract, the clearance rate of the soothing repair essence to free radicals is obviously improved, the compounding of the six plant extracts can obviously improve the oxidation resistance of the purslane extract, and in the example 10, the improvement of the oxidation resistance of the purslane extract is not only dependent on the function of the trifingerlarea extract for promoting the efficient absorption of other components.

Experiment 3

And judging the continuous moisturizing effect of the soothing and repairing essence by using a skin detector. The moisture-preserving effect of the soothing and repairing essence can be judged by detecting the water content of the stratum corneum, the repairing effect of the soothing and repairing essence on the skin barrier can be judged by detecting the TEWL value, and the smaller the TEWL value is, the better the skin barrier function is shown.

Experimental equipment: ASA-M100 skin stratum corneum moisture content tester (purchased from guining (shanghai) experimental equipment ltd.) and ASCH percutaneous moisture loss tester (purchased from guining (shanghai) experimental equipment ltd.).

The experimental method comprises the following steps: the test persons were divided into 18 groups of 5 persons each. Each group of testers respectively uses the soothing and repairing essence lotion in each embodiment and comparative example in the morning and evening, before each use, the face needs to be cleaned, then the soothing and repairing essence lotion is uniformly smeared on the face, then the face is beaten by gently massaging hands for 2min, and the testers need to continuously use the soothing and repairing essence lotion for 28 days.

Before use, on the 28 th day after use, the water content of the horny layer in the same area of the skin of a tester is detected by an ASA-M100 horny layer water content tester according to the formula: the water content increase rate (%) of the skin stratum corneum = [ (water content of skin stratum corneum after use-water content of skin stratum corneum before use)/water content of skin stratum corneum before use ] × 100%, the water content increase rate of the skin stratum corneum was calculated, and the experimental results are shown in table 4.

The TEWL values of the same area of the skin of the test subjects were measured using the ASCH transdermal Water loss Meter before, on days 2, 7, 14, 21, and 28, respectively, and the results are shown in Table 5.

Table 4 skin cuticle water content increase rate (%)

TABLE 5TEWL values

Group of	Before use	2 days	7 days	14 days	21 days	28 days
							Example 1	23.78	19.21	18.11	16.74	15.34	14.00
Example 2	23.76	19.19	18.09	16.72	15.33	13.98
							Example 3	23.83	19.25	18.14	16.77	15.37	14.02
Example 4	23.15	18.81	17.67	16.27	14.83	13.51
							Example 5	23.54	18.38	17.00	15.55	14.07	12.59
Example 6	23.47	18.44	17.06	15.60	14.12	12.63
							Example 7	23.49	17.92	16.48	15.07	13.59	12.09
Example 8	23.41	17.95	16.24	14.64	13.09	11.47
							Example 9	23.45	17.94	16.23	14.64	13.09	11.46
Example 10	23.82	17.17	15.54	14.01	12.53	10.97
							Comparative example 1	23.79	22.09	21.76	21.14	20.32	19.32
Comparative example 2	23.72	22.44	22.10	21.47	20.64	19.63
							Comparative example 3	23.42	22.13	21.80	21.18	20.36	19.36
Comparative example 4	23.66	19.17	18.05	16.67	15.26	13.93
							Comparative example 5	23.75	19.16	18.04	16.64	15.23	13.90
Comparative example 6	23.84	21.51	20.99	20.45	19.42	18.30
							Comparative example 7	23.77	19.40	18.48	17.23	15.88	14.60
Comparative example 8	23.46	19.49	18.56	17.31	15.95	14.67

By comparing the example 4 with the comparative examples 1 to 3, the soothing and repairing essence prepared by compounding the yucca schidigera extract, the tiramer aristolochia extract and the cactus macrocephala extract can obviously improve the continuous moisturizing effect of the skin, and can maintain the water content of the skin horny layer by promoting the generation of skin moisturizing factors, so that the repairing effect of the skin barrier is better.

By comparing the example 4 with the examples 5-7, it can be shown that the soothing repair essence prepared by compounding the 5 plant extracts of the yucca brevifolia root extract, the three-finger larry-shaped largeflower prickly pear extract, the ampelopsis grossedentata extract and the peony root bark extract can accelerate the repair level of skin sebum membranes by improving the oxidation resistance of the skin, thereby further improving the water-locking capacity of the skin and accelerating the repair speed of skin barriers.

By comparing the example 7 with the examples 8-10, the soothing and repairing essence prepared by compounding the six plant extracts of yucca brevicaulis root extract, trimeresurus extract, opuntia ficus-indica extract, ampelopsis grossedentata extract, paeonia suffruticosa root bark extract and purslane extract can further improve the oxidation resistance of skin, thereby further improving the water locking capacity of the skin and the repairing speed of skin barriers.

Through comparison between the example 7 and the comparative examples 4-5, it can be shown that the skin antioxidation improving capability of the soothing and repairing essence prepared by compounding the 5 plant extracts including the yucca brevifolia root extract, the sanzhireia extract, the opuntia ficus indica extract, the ampelopsis grossedentata extract and the peony root bark extract is not only the superposition of the self-contained efficacies of the 5 plant extracts, but also the skin antioxidation can be further improved by the soothing and repairing essence prepared by compounding the 5 plant extracts, so that the skin barrier can be repaired better and faster.

By comparing the example 10 with the comparative example 6, it can be shown that the skin antioxidation improvement capability of the soothing and repairing essence compounded by the six plant extracts, namely, the yucca schidigera extract, the tridactylus extract, the ampelopsis grossedentata extract, the peony root bark extract and the purslane herb extract, is not only dependent on the efficient absorption of other components by the tridactylus extract, but also proves that the soothing and repairing essence compounded by the six plant extracts can further promote the skin antioxidation, so that the skin sebum membrane can be repaired more efficiently, the skin barrier repair speed is faster, and the sensitive muscle can be recovered more quickly. The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the principles of the present application should be covered by the protection scope of the present application.

Claims

1. The soothing and repairing essence is characterized by comprising the following components in percentage by mass:

humectant: 11.50% -12.50%;

antioxidant: 0.40% -0.60%;

chelating agent: 0.015% -0.025%;

thickening agent: 0.095% -0.115%;

skin conditioner: 0.60% -0.65%;

plant extracts: 0.60% -6.75%;

the balance of water;

the plant extract comprises yucca brevifolia root extract, trimeresurus reyi extract and Opuntia ficus-indica extract.

2. The soothing repair essence according to claim 1, wherein the yucca schidigera extract accounts for 0.25-0.27% of the soothing repair essence;

the weight percentage of the tridactylari extract in the soothing and repairing essence is 0.25% -0.28%;

the mass percentage of the opuntia ficus-indica extract in the soothing and repairing essence is 0.10% -0.13%.

3. The soothing and restoring essence according to claim 2, wherein the mass ratio of the yucca brevifolia root extract, the tiria arabica extract and the opuntia ficus-indica extract in the soothing and restoring essence is 5:5:2.

4. the soothing repair serum of claim 1 wherein the plant extract further comprises ampelopsis grossedentata extract and peony root bark extract.

5. The soothing and repairing essence of claim 4, wherein the weight percentage of the ampelopsis grossedentata extract in the soothing and repairing essence is 0.10% -0.11%;

the mass percentage of the peony root-bark extract in the soothing and repairing essence is 0.45-0.50%.

6. The soothing repair essence according to claim 4, wherein the mass ratio of yucca brevifolia root extract, trimeresura extract, opuntia ficus indica extract, ampelopsis grossedentata extract and peony root bark extract in the soothing repair essence is 5:5:2:2:10.

7. the soothing repair serum of claim 4 wherein the botanical extract further comprises purslane extract.

8. The soothing repair essence lotion of claim 7, wherein the mass percentage of the purslane extract in the soothing repair essence lotion is 5.0-5.5%.

9. A soothing repair essence according to claims 1 to 8, wherein the skin conditioner comprises sodium hyaluronate with a molecular weight of less than 10000Da and D-panthenol.

10. The soothing repair essence lotion according to claim 9, wherein the mass percentage of the sodium hyaluronate with the molecular weight less than 10000Da in the soothing repair essence lotion is 0.10% -0.11%;

the mass percentage of the D-panthenol in the soothing repair essence lotion is 0.50-0.52%.