CN115517999B

CN115517999B - Anti-aging composition and application thereof in beauty and skin care

Info

Publication number: CN115517999B
Application number: CN202211319447.6A
Authority: CN
Inventors: 雷楚屏
Original assignee: Guangzhou Aizhuo Biotechnology Co ltd
Current assignee: Guangzhou Aizhuo Biotechnology Co ltd
Priority date: 2022-10-26
Filing date: 2022-10-26
Publication date: 2023-09-15
Anticipated expiration: 2042-10-26
Also published as: CN115517999A

Abstract

The application discloses an anti-aging composition and application thereof in beauty and skin care, wherein the anti-aging composition comprises the following raw materials in parts by mass: 0.1-5 parts of antioxidant, 0.0001-0.05 part of compound polypeptide, 1-10 parts of panthenol and 0.3-2 parts of glycoside compound; the antioxidant is at least one selected from arbutin, ergothioneine, grape seed extract, olive leaf extract, glabridin, retinol and its derivatives, ascorbic acid and its derivatives; the compound polypeptide is at least one of arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1 and acetyl hexapeptide-1; the glycoside compound is at least one selected from glyceroglycosides, glucopyranosides, glucosan and sodium hyaluronate. The cosmetic prepared from the anti-aging composition is matched with a radio frequency beauty instrument for use, can promote the regeneration of skin dermis collagen, and is filled with and desalts fine wrinkles, so that the effects of deep anti-aging, long-acting moisturizing, anti-wrinkle and skin nourishing are achieved.

Description

Anti-aging composition and application thereof in beauty and skin care

Technical Field

The application belongs to the field of cosmetics, and particularly relates to an anti-aging composition and application thereof in beauty and skin care.

Background

In the new medical and cosmetic field of skin care, radio frequency is a cosmetic technology that has been eagerly sought by market consumers in recent years. The radio frequency generates a thermal effect on tissue friction through which the current passes by utilizing the polarity conversion of the electric field, and the current is mainly accumulated in the dermis layer due to the highest dermal conductivity in the skin tissue, so that the dermis tissue is heated in a large volume. When the heating temperature reaches 60 ℃, the collagen is instantly contracted, when the heating temperature reaches 42 ℃, the conformation change of the helical structure of the collagen is caused, the wound repair mechanism of the organism can be further started, and fibroblasts are stimulated to produce the synthesis of new collagen, elasticity and other supporting proteins, so that the effects of tightening skin, removing wrinkles, lifting and pulling, improving skin quality and contour and the like are obtained.

The brand of powerful radio-frequency beauty instrument can carry out careful study on parameters such as radio-frequency, instrument efficacy, radio-frequency action depth and the like, and aims at enabling energy acting on dermis to be faster and larger, but excessive energy is easy to cause thermal damage to healthy cells and surrounding tissue cells, oxidative free radicals are generated, and even normal metabolism of skin can be directly influenced. Taking heat-Marji radio frequency as an example, customers typically experience a repair period of about 3-10 days after surgery, such as drying, redness, swelling, crusting, etc.

In addition, the mechanism of action of the radio frequency cosmetic device determines that the dermal layer fibroblasts synthesize more extracellular matrix, but these newly synthesized extracellular matrices are not uniformly distributed. For this reason, fibroblasts are living and can sense and respond to mechanical forces, and secreted collagen fibers form stronger bundles along the direction of the mechanical force to counter the mechanical force. The force non-uniformity is more pronounced, and is also known as chinese character, fish tail, head line, etc. As the age increases, the wrinkles are more and more obvious, so that the radio frequency beauty instrument only solves the problems of collagen synthesis and collagen arrangement, and further has limited effect of improving the wrinkles such as expression lines

Therefore, the application discloses an anti-aging composition with good moisturizing, antioxidation and neurotransmitter conduction inhibiting effects, which has important significance for exerting the anti-aging and beautifying effects of the radio frequency beautifying instrument.

Disclosure of Invention

In view of the above-mentioned shortcomings in the prior art, it is an object of the present application to provide an anti-aging composition. The anti-aging composition provided by the application adopts safe and nontoxic raw materials, has no side effect and mild formula. The anti-aging composition is obtained by compounding the antioxidant, the compound polypeptide, the panthenol and the glycoside compound in a specific proportion, the anti-wrinkle performance of the composition can be obviously improved, and the cosmetics prepared from the anti-aging composition have the effects of resisting oxidation, long-acting moisturizing, resisting aging, resisting bacteria and the like. The prepared cosmetics are matched with a radio frequency beauty instrument for use, can promote the regeneration of skin and dermis collagen, and fill and fade fine wrinkles, thereby achieving the effects of deep anti-aging, long-acting moisture preservation, anti-wrinkle and skin nourishing.

The application aims to provide an anti-aging composition, which comprises the following raw materials in parts by mass:

0.1-5 parts of antioxidant;

0.0001-0.05 part of compound polypeptide;

1-10 parts of panthenol;

0.3-2 parts of glycoside compound;

the antioxidant is at least one selected from arbutin, ergothioneine, grape seed extract, olive leaf extract, glabridin, retinol and its derivatives, ascorbic acid and its derivatives;

the compound polypeptide is at least one of arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1 and acetyl hexapeptide-1;

the glycoside compound is at least one selected from glyceroglycosides, glucopyranosides, glucosan and sodium hyaluronate.

Arbutin, also known as arbutin, is widely available in plants of the genus Arbutea, is easily extracted, can be artificially synthesized, and has low toxicity. In the cytological experiment, through cell morphological observation and biochemical index detection, RT-PCR is adopted to verify the relation between the arbutin and Grx-mRNA expression, the arbutin has a certain antioxidation effect, and simultaneously has the effects of inhibiting the activity of tyrosinase in vivo, preventing the generation of melanin and sterilizing and diminishing inflammation.

Ergothioneine is a natural antioxidant, can protect cells in human body, is an important active substance in the body, and has various physiological functions of scavenging free radicals, whitening skin, resisting aging, preventing ultraviolet radiation injury, maintaining DNA synthesis and normal growth of cells, etc.

Grape seed extract is a novel high-efficiency natural antioxidant substance extracted from grape seeds, which cannot be synthesized in human body. It can effectively remove redundant free radicals in human body, and has the functions of superstrong delaying senility and enhancing immunity. Has effects of resisting oxidation, resisting allergy, relieving fatigue, strengthening body constitution, improving sub-health state, delaying aging, improving dysphoria, irritability, dizziness, debilitation, and hypomnesis.

The olive leaf extract mainly comprises effective components such as oleuropein, hydroxytyrosol and the like, wherein the oleuropein is a nontoxic secoiridoid compound. Studies have shown that. Oleuropein has antifungal, antiinflammatory, antiviral, antioxidant, anticancer and blood glucose reducing effects. Oleuropein is also the most bioactive component, has strong antioxidant activity in vitro, and the strong antioxidant activity is derived from a structure with a substituted dihydroxy at a clinical position, and can be combined with superoxide anion, hydrogen peroxide, nitric oxide free radical and nitro free radical to remove the free radical. Hydroxytyrosol has catechol structure, is typical antioxidant stress substance and free radical scavenger, and is shown by one authoritative data of the torrenk laboratory in boston, ma, usa that the ORAC antioxidant adsorption free radical energy value of hydroxytyrosol is more than 10 times higher than that of vitamin C and more than 4-6 times higher than that of anthocyanin.

Glabridin has the effects of resisting oxidation and aging, is mainly present at the root and stem of glabra, has strong capability of scavenging free radicals, has a certain effect of whitening skin, and is easy to deteriorate in a high-temperature environment. In addition, glabridin also has a certain anti-inflammatory effect, and can be used for relieving inflammation and whitening skin pigmentation.

Retinol can help the skin stratum corneum to restore normal metabolism, thicken the skin stratum corneum, strengthen the water storage capacity of the skin, and promote collagen generation of the dermis layer of the skin; has effects of reducing pores, resisting aging, removing wrinkle, eliminating acne, and whitening skin.

Ascorbic acid and its derivatives are very efficient antioxidants, and because ascorbic acid is easily oxidized to produce dehydroascorbic acid when exposed to air or aqueous solution, more stable ascorbic acid derivatives such as magnesium ascorbyl phosphate, tetraisopalmitate, ethyl ascorbate and the like are commonly used in cosmetics, and ascorbic acid is produced after enzymolysis in skin, thereby playing roles in assisting in synthesizing collagenase, photoprotection, inhibiting tyrosinase and resisting photoaging.

Glycerol glucoside is a class of glycoside compounds formed by connecting glycerol molecules and glucose molecules through glycosidic bonds, wherein the structure with important physiological functions is in 2-alpha configuration. The glyceroglycosides are emergency products of plants under extreme conditions, are favorable for protecting the integrity of cell membranes under extreme conditions, and can promote the expression of skin aquaporin AQP3, enhance the moisturizing ability of skin, promote the growth of skin cells, accelerate the repair and metabolism of cells, resist inflammation and the like in skin care products.

The glucopyranoside has a polycyclic structure and the structural formula is shown as follows:

dextran refers to a homotype polysaccharide consisting of glucose as monosaccharide, and glucose units are connected through glycosidic bonds.

Sodium hyaluronate belongs to a high molecular polymer and has strong lubricating feel and film forming property, so that the skin care product containing the sodium hyaluronate has obvious lubricating feel and good hand feeling when being smeared. The macromolecular sodium hyaluronate forms a layer of breathable film on the surface of the skin, so that the skin is smooth and moist, and invasion of foreign bacteria, dust and the like is prevented; the sodium hyaluronate with smaller molecular weight can permeate into dermis layer, has the functions of slightly expanding capillary vessel, increasing blood circulation, improving intermediate metabolism, promoting skin nutrition absorption and waste excretion, thereby preventing skin aging and playing a role in maintaining beauty and keeping young.

Panthenol is also called panthenol, provitamin B5, colorless to yellowish transparent viscous liquid. Panthenol has effects of preventing rough skin and aging. Also has obvious anti-inflammatory effect, can delay the progress of inflammatory reaction, and is an ideal auxiliary anti-acne component for sensitive muscles or oilpox muscles.

Preferably, the anti-aging composition comprises the following raw materials in parts by mass:

1-4 parts of an antioxidant;

0.001-0.03 part of composite polypeptide;

3-8 parts of panthenol;

0.5-1.5 parts of glycoside compound;

More preferably, the anti-aging composition comprises the following raw materials in parts by mass:

2 parts of an antioxidant;

0.01 part of composite polypeptide;

5 parts of panthenol;

glycoside compounds 1 part;

Preferably, the extraction process of the olive leaf extract comprises the following steps:

pulverizing folium Canarii albi, adding water to extract water-soluble components, filtering, centrifuging, collecting supernatant, chromatography, concentrating at low temperature, and drying to obtain folium Canarii albi extract powder.

Preferably, the anti-aging composition further comprises 1-20 parts of polyhydroxy humectant with carbon chain length of C3-C6.

More preferably, the anti-aging composition further comprises 10 parts of a polyhydroxy humectant having a carbon chain length of C3-C6.

Preferably, the polyhydroxy humectant with carbon chain length of C3-C6 is selected from at least one of glycerin, propylene glycol, butylene glycol, methyl propylene glycol, rhamnose, erythritol, arabinose and saccharide isomer.

More preferably, the polyhydroxy humectant with carbon chain length of C3-C6 is at least one selected from glycerol and rhamnose.

The glycerol selected by the application has good water retention capacity and skin surface permeability; rhamnose has effect in promoting proliferation of keratinocyte, and can promote hemidesmosome structure. The semidesmosomes are mainly positioned on the bottom surface of the epithelial cells and serve to connect the epithelial cells with the underlying basal membrane, thus enhancing the barrier function of the skin.

The application further aims to provide an application of the anti-aging composition in preparing cosmetics, wherein the cosmetics contain the anti-aging composition, and the cosmetics are selected from any one of mask liquid, emulsion, cream, toner, essence, stock solution, face cleansing emulsion, toning lotion, perfume, makeup remover, foundation solution, foundation cream and concealer.

Preferably, the anti-aging composition is used in an amount of 5-40% by mass of the cosmetic.

It is a further object of the present application to provide the use of said anti-aging composition in radiofrequency cosmetology and in medical skin care.

The skin care product is singly used, the functional components of the skin care product are usually blocked by the skin barrier of a human body, most of the functional components stay in the epidermis horny layer, hardly penetrate through the epidermis layer, or the concentration of the effective components penetrating into the dermis layer of the skin is too low, so that fibroblasts are not stimulated to generate more collagen, elastic fibers and extracellular matrix, the dermis layer thickness is not obviously changed, and therefore, the antioxidation and anti-aging effects are very limited. The anti-aging composition is applied to the radio frequency beauty skin care, and energy generated by a radio frequency instrument stimulates the dermis layer to generate more extracellular matrixes, and meanwhile, the hydration degree of the epidermis layer can be accelerated, the osmotic pressure of a contact surface is increased, and the transdermal absorption of active ingredients of cosmetics is promoted.

Compared with the prior art, the application has the following beneficial effects:

(1) The composite polypeptide selected by the application blocks sodium ion inflow channel Nav1.4 of muscle fiber, simultaneously regulates calcium ion inflow, prevents muscle operation potential from forming, further reduces muscle contraction, simulates the N-terminal end of SANP-25, participates in competing SNAP-25 at the site of a foam-melting complex, influences the formation of the complex, and finally inhibits/blocks the conduction path of neurotransmitter, thereby preventing and removing dynamic expression lines, and reducing the depth and density of wrinkles.

(2) According to the application, the anti-aging composition is obtained by compounding the antioxidant, the compound polypeptide, the panthenol and the glycoside compound in a specific proportion, the anti-wrinkle performance of the composition can be obviously improved, and the cosmetics prepared from the anti-aging composition have the effects of resisting oxidation, keeping moisture for a long time, stretching wrinkles and the like.

(3) The cosmetics prepared from the antioxidant, the compound polypeptide, the panthenol and the glycoside compound in a specific proportion are matched with a radio frequency beauty instrument for use, so that the skin and dermis collagen can be promoted to be regenerated, fine lines are filled and desalted, and the effects of deep anti-aging, long-acting moisturizing, anti-wrinkle and skin nourishing are achieved.

(4) The anti-aging composition adopted by the application has the advantages of wide raw material sources, safety, no toxicity, no side effect and mild formula.

Detailed Description

In order to make the technical solution of the present application better understood by those skilled in the art, the technical solution of the present application will be clearly and completely described in conjunction with the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.

Grape seed extract (grape seed procyanidine greater than 95%) was purchased from Gui Linlai strain biotechnology Co., ltd

Example 1: the extraction process of the olive leaf extract comprises the following steps:

pulverizing folium Canarii albi with pulverizer, adding deionized water 3-5 times of the mass of the pulverized material to extract water-soluble components, filtering with three layers, centrifuging the filtrate at high speed, collecting supernatant, subjecting to resin chromatography to obtain primary product of folium Canarii albi extract, concentrating the primary product at low temperature, drying, and spraying powder to obtain folium Canarii albi extract powder.

Example 2: preparation of the anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.0005g of arginine/lysine polypeptide, 0.0005g of acetyl hexapeptide-8.0005 g of glyceroglucose, 0.1g of panthenol and 1g of glycerol to obtain the anti-aging composition.

Example 3: preparation of the anti-aging composition.

Mixing ergothioneine 0.2g, acetyl hexapeptide-1.001 g, glucopyranoside 0.1g, panthenol 0.5g, and glycerol 1g, and obtaining antiaging composition.

Example 4: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.001g of acetyl octapeptide-3, 0.1g of glucan, 0.5g of panthenol and 1g of glycerol to obtain the anti-aging composition.

Example 5: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.0003g of acetyl octapeptide-3, 0.0007g of acetyl hexapeptide-1, 0.1g of sodium hyaluronate, 0.5g of panthenol and 1g of glycerin to obtain the anti-aging composition.

Example 6: preparation of the anti-aging composition.

Mixing grape seed extract 0.2g, acetyl octapeptide-3.0006 g, acetyl hexapeptide-1.0004 g, glyceroglucoside 0.1g, panthenol 0.5g, and glycerol 1g to obtain antiaging composition.

Example 7: preparation of the anti-aging composition.

Mixing olive leaf extract 0.2g, acetyl octapeptide-3.0004 g, acetyl hexapeptide-1.0006 g, glyceroglucoside 0.1g, panthenol 0.5g, and glycerol 1g to obtain antiaging composition.

Example 8: preparation of the anti-aging composition.

Uniformly mixing 0.0062g of retinol, 0.0002g of acetyl octapeptide-3, 0.0008g of acetyl hexapeptide-1, 0.1038g of glyceroglucoside, 0.69g of panthenol and 1g of glycerol to obtain the anti-aging composition.

Example 9: preparation of the anti-aging composition.

Uniformly mixing 0.2g of ascorbyl ethyl ether, 0.0007g of acetyl hexapeptide-8.0003 g of acetyl hexapeptide-1.0003 g of glycerin glucoside 0.1g, 0.5g of panthenol and 1g of glycerin to obtain the anti-aging composition.

Example 10: preparation of the anti-aging composition.

0.2g of ergothioneine, 0.001g of tripeptide-1.001 g of dextran, 0.04g of glyceroglucoside, 0.5g of panthenol and 1g of glycerin are uniformly mixed to obtain the anti-aging composition.

Example 11: preparation of the anti-aging composition.

Mixing 0.1g of olive leaf extract, 0.1g of glabridin, 0.001g of pentapeptide-3, 0.1g of glyceroglucoside, 0.5g of panthenol and 1g of glycerol uniformly to obtain the anti-aging composition.

Comparative example 1: preparation of the anti-aging composition.

Mixing arbutin 0.2g and glycerin 1.601g uniformly to obtain the antiaging composition.

Comparative example 2: preparation of the anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.0005g of arginine/lysine polypeptide, 0.0005g of acetyl hexapeptide-8, 0.1g of glyceroglycoside and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 3: preparation of the anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.1g of glyceroglucose, 0.5g of panthenol and 1.001g of glycerol to obtain the anti-aging composition.

Comparative example 4: preparation of the anti-aging composition.

Uniformly mixing 0.2g of arbutin, 0.0005g of arginine/lysine polypeptide, 0.0005g of acetyl hexapeptide-8, 0.5g of panthenol and 1.1g of glycerin to obtain the anti-aging composition.

Comparative example 5: preparation of the anti-aging composition.

And uniformly mixing 0.2g of ergothioneine and 1.601g of glycerol to obtain the anti-aging composition.

Comparative example 6: preparation of the anti-aging composition.

Mixing ergothioneine 0.2g, acetyl hexapeptide-1.001 g, glucopyranoside 0.1g, and glycerol 1.5g, and obtaining antiaging composition.

Comparative example 7: preparation of the anti-aging composition.

Mixing ergothioneine 0.2g, acetyl hexapeptide-1.001 g, panthenol 0.5g, and glycerol 1.1g, and obtaining antiaging composition.

Comparative example 8: preparation of the anti-aging composition.

Mixing ergothioneine 0.2g, glucopyranoside 0.1g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 9: preparation of the anti-aging composition.

And uniformly mixing 0.2g of glabridin and 1.601g of glycerol to obtain the anti-aging composition.

Comparative example 10: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.001g of acetyl octapeptide-3.001 g of glucan, 0.1g of glycerol and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 11: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.001g of acetyl octapeptide-3.001g, 0.5g of panthenol and 1.1g of glycerol to obtain the anti-aging composition.

Comparative example 12: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.1g of glucan, 0.5g of panthenol and 1.001g of glycerol to obtain the anti-aging composition.

Comparative example 13: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.0003g of acetyl octapeptide-3, 0.0007g of acetyl hexapeptide-1, 0.1g of sodium hyaluronate and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 14: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.0003g of acetyl octapeptide-3, 0.0007g of acetyl hexapeptide-1.0007 g of panthenol 0.5g and 1.1g of glycerin to obtain the anti-aging composition.

Comparative example 15: preparation of the anti-aging composition.

Uniformly mixing 0.2g of glabridin, 0.1g of sodium hyaluronate, 0.5g of panthenol and 1.001g of glycerin to obtain the anti-aging composition.

Comparative example 16: preparation of the anti-aging composition.

Mixing grape seed extract 0.2g and glycerol 1.601g uniformly to obtain the antiaging composition.

Comparative example 17: preparation of the anti-aging composition.

Mixing grape seed extract 0.2g, acetyl octapeptide-3.0006 g, acetyl hexapeptide-1.0004 g, glyceroglycoside 0.1g, and glycerol 1.5g to obtain antiaging composition.

Comparative example 18: preparation of the anti-aging composition.

Mixing grape seed extract 0.2g, acetyl octapeptide-3.0006 g, acetyl hexapeptide-1.0004 g, panthenol 0.5g, and glycerol 1.1g to obtain antiaging composition.

Comparative example 19: preparation of the anti-aging composition.

Mixing grape seed extract 0.2g, glyceroglucoside 0.1g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 20: preparation of the anti-aging composition.

Mixing 0.2g of olive leaf extract and 1.601g of glycerol uniformly to obtain the anti-aging composition.

Comparative example 21: preparation of the anti-aging composition.

Mixing olive leaf extract 0.2g, acetyl octapeptide-3.0004 g, acetyl hexapeptide-1.0006 g, glyceroglyceoside 0.1g, and glycerol 1.5g to obtain antiaging composition.

Comparative example 22: preparation of the anti-aging composition.

Mixing olive leaf extract 0.2g, acetyl octapeptide-3.0004 g, acetyl hexapeptide-1.0006 g, panthenol 0.5g and glycerol 1.1g to obtain antiaging composition.

Comparative example 23: preparation of the anti-aging composition.

Mixing olive leaf extract 0.2g, glyceroglucoside 0.1g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 24: preparation of the anti-aging composition.

Mixing retinol 0.0062g and glycerin 1.7948g uniformly to obtain the antiaging composition.

Comparative example 25: preparation of the anti-aging composition.

Uniformly mixing 0.0062g of retinol, 0.0002g of acetyl octapeptide-3, 0.0008g of acetyl hexapeptide-1, 0.1038g of glyceroglycoside and 1.69g of glycerol to obtain the anti-aging composition.

Comparative example 26: preparation of the anti-aging composition.

Uniformly mixing 0.0062g of retinol, 0.0002g of acetyl octapeptide-3, 0.0008g of acetyl hexapeptide-1, 0.69g of panthenol and 1.1038g of glycerin to obtain the anti-aging composition.

Comparative example 27: preparation of the anti-aging composition.

Uniformly mixing 0.0062g of retinol, 0.1038g of glyceroglycoside, 0.69g of panthenol and 1.001g of glycerol to obtain the anti-aging composition.

Comparative example 28: preparation of the anti-aging composition.

And uniformly mixing 0.2g of ascorbic acid ethyl ether and 1.601g of glycerol to obtain the anti-aging composition.

Comparative example 29: preparation of the anti-aging composition.

Uniformly mixing 0.2g of ascorbyl ethyl ether, 0.0007g of acetyl hexapeptide-8.0003 g of acetyl hexapeptide-1.0003 g of glycerol glucoside 0.1g and 1.5g of glycerol to obtain the anti-aging composition.

Comparative example 30: preparation of the anti-aging composition.

Uniformly mixing 0.2g of ascorbyl ethyl ether, 0.0007g of acetyl hexapeptide-8.0003 g of acetyl hexapeptide-1.0003 g of panthenol 0.5g and 1.1g of glycerin to obtain the anti-aging composition.

Comparative example 31: preparation of the anti-aging composition.

Uniformly mixing 0.2g of ascorbyl ethyl ether, 0.1g of glyceroglucoside, 0.5g of panthenol and 1.001g of glycerol to obtain the anti-aging composition.

Comparative example 32: preparation of the anti-aging composition.

0.2g of ergothioneine, 0.001g of tripeptide-1, 0.06g of glucan, 0.04g of glyceroglycoside and 1.5g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 33: preparation of the anti-aging composition.

0.2g of ergothioneine, 0.001g of tripeptide-1, 0.5g of panthenol and 1.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 34: preparation of the anti-aging composition.

Mixing ergothioneine 0.2g, dextran 0.06g, glyceroglucoside 0.04g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

Comparative example 35: preparation of the anti-aging composition.

Mixing 0.1g of olive leaf extract, 0.1g of glabridin, 0.001g of pentapeptide-3, 0.1g of glyceroglycoside and 1.5g of glycerol uniformly to obtain the anti-aging composition.

Comparative example 36: preparation of the anti-aging composition.

Mixing 0.1g of olive leaf extract, 0.1g of glabridin, 0.001g of pentapeptide-3, 0.5g of panthenol and 1.1g of glycerol uniformly to obtain the anti-aging composition.

Comparative example 37: preparation of the anti-aging composition.

Mixing olive leaf extract 0.1g, glabridin 0.1g, glyceroglucoside 0.1g, panthenol 0.5g, and glycerol 1.001g to obtain antiaging composition.

DPPH radical scavenging ability assay:

the testing steps are as follows: a certain amount of DPPH was weighed and a DPPH solution of 0.1mg/mL was prepared with absolute ethanol. 2mL of the anti-aging compositions of examples 2 to 11 and comparative examples 1 to 37 are respectively taken, 1000g of absolute ethyl alcohol is added for diluting the sample liquid, 2mL of DPPH solution is added, the mixture is uniformly mixed, the reaction is carried out at room temperature for 30min, and then the light absorption value Ai is measured at 517 nm. And simultaneously measuring absorbance A0 of 2mL of DPPH solution and 2mL of absolute ethyl alcohol and absorbance Aj of 2mL of sample solution and 2mL of absolute ethyl alcohol after mixing. The assay was repeated 2 times. The clearance of the sample to DPPH radicals was calculated using the following formula: the experimental results are shown in Table 1.

DPPH radical scavenging = (a0-aj+ai)/a0×100%

The evaluation method comprises the following steps: the antioxidant capacity can be reflected by DPPH radical scavenging ability measurement. The higher the DPPH radical scavenging rate, the stronger the antioxidant capacity and conversely the weaker the antioxidant capacity.

Table 1 DPPH radical scavenging test results for the anti-aging compositions of examples 2-11 and comparative examples 1-37.

According to detection, arginine/lysine polypeptide, acetyl octapeptide-3, acetyl hexapeptide-8, pentapeptide-3, tripeptide-1, acetyl hexapeptide-1, glyceroglycosides, glucopyranosides, dextran, sodium hyaluronate, panthenol, glycerol, propylene glycol, butylene glycol, methyl propylene glycol, rhamnose, erythritol, arabinose and saccharide isomers have no DPPH free radical scavenging ability.

As can be seen from table 1, by comparing examples 2 and 1-4, examples 3 and 5-8, examples 4 and 9-12, examples 5 and 9, 13-15, examples 6 and 16-19, examples 7 and 20-23, examples 8 and 24-27, examples 9 and 28-31, examples 10 and 5, 32-34, and examples 11 and 20, 35-37, it can be found that the compounding of the compound polypeptide, panthenol and glycoside compound in a specific ratio can promote the antioxidant effect of the antioxidant, while the compounding of any two of the compound polypeptide, panthenol and glycoside compound can not effectively promote the antioxidant effect of the antioxidant. Meanwhile, the compound polypeptide, panthenol and glycoside compounds outside the specific proportion of the application can not effectively promote the antioxidation effect of the antioxidant. The antioxidant, the compound polypeptide, the panthenol and the glycoside compounds in a specific proportion are mixed with other moisturizers in the application, and the obtained anti-aging composition also has the same action and effects as those of examples 2-11.

Comparative example 38: preparation of the anti-aging composition.

Mixing arbutin 0.0801g and glycerin 0.1g uniformly to obtain the antiaging composition.

Comparative example 39: preparation of the anti-aging composition.

0.0801g of ergothioneine and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 40: preparation of the anti-aging composition.

Mixing grape seed extract 0.0801g and glycerol 0.1g uniformly to obtain the antiaging composition.

Comparative example 41: preparation of the anti-aging composition.

0.0801g of olive leaf extract and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 42: preparation of the anti-aging composition.

Mixing glabridin 0.0801g and glycerol 0.1g uniformly to obtain the antiaging composition.

Comparative example 43: preparation of the anti-aging composition.

Mixing retinol 0.0062g and glycerin 0.00774g uniformly to obtain the antiaging composition.

Comparative example 44: preparation of the anti-aging composition.

0.0801g of ascorbic acid and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 45: preparation of the anti-aging composition.

0.0801g of arginine/lysine polypeptide and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 46: preparation of the anti-aging composition.

And uniformly mixing 0.0801g of acetyl octapeptide-3 and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 47: preparation of the anti-aging composition.

And uniformly mixing 0.0801g of acetyl hexapeptide-8 and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 48: preparation of the anti-aging composition.

Mixing pentapeptide-3 0.0801g and glycerin 0.1g to obtain antiaging composition.

Comparative example 49: preparation of the anti-aging composition.

And uniformly mixing 0.0801g of tripeptide-1 and 0.1g of glycerol to obtain the anti-aging composition.

Comparative example 50: preparation of the anti-aging composition.

And uniformly mixing 0.0801g of acetyl hexapeptide-1 and 0.1g of glycerin to obtain the anti-aging composition.

Comparative example 51: preparation of the anti-aging composition.

0.0801g of glyceroglucoside and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 52: preparation of the anti-aging composition.

Mixing glucopyranoside 0.0801g and glycerin 0.1g uniformly to obtain the antiaging composition.

Comparative example 53: preparation of the anti-aging composition.

Mixing dextran 0.0801g and glycerol 0.1g uniformly to obtain the antiaging composition.

Comparative example 54: preparation of the anti-aging composition.

0.0801g of sodium hyaluronate and 0.1g of glycerol are uniformly mixed to obtain the anti-aging composition.

Comparative example 55: preparation of the anti-aging composition.

Mixing panthenol 0.0801g and glycerol 0.1g uniformly to obtain the antiaging composition.

Preparation of essence:

(1) The anti-aging compositions prepared in examples 2 to 11 and comparative examples 38 to 55 were prepared as essential oils according to the formulation of Table 2, respectively, to obtain essential oils of examples 12 to 21 and comparative examples 56 to 73, respectively.

Table 2. Essence formulation.

(2) The preparation method of the essence comprises the following steps:

A. adding deionized water into a stirring pot, adding the ammonium acryloyldimethyl taurate/VP copolymer and xanthan gum into the stirring pot, stirring until the mixture is completely and uniformly dispersed, heating to 70-85 ℃, and homogenizing at high speed for 5min.

B. Adding polydimethylsiloxane and OLIEVM 1000, mixing, adding the anti-aging compositions of examples 2-11 and comparative examples 38-55, stirring, heating to 40-55deg.C, dissolving completely, homogenizing at high speed for 5min, cooling to 40deg.C, adding essence and hydrolyzed oat protein, and stirring to uniformity to obtain essence.

Anti-aging performance test:

285 skin healthy volunteers aged 30-50 years were selected and randomized equally into 57 groups of 5.

The mask liquid is matched with a radio frequency beauty instrument for use: after 140 volunteers are randomly selected and cleaned, the essences of examples 12-21 and comparative examples 56-73 are respectively smeared on the faces and the corners of eyes of the volunteers, and the essences are dispersed and absorbed at the faces and the corners of eyes by using a radio frequency beauty instrument for 20min; 3 times per week for 4 weeks; the average of the corresponding indicators after 4 weeks of the test is recorded.

Only mask liquid is used: randomly selecting 140 volunteers, cleaning the face, respectively taking the essence of examples 12-21 and comparative examples 56-73, respectively coating the essence on the face and the canthus of the volunteers, and absorbing the essence on the face and the canthus for 20min; 3 times per week for 4 weeks; the average of the corresponding indicators after 4 weeks of the test is recorded.

Using only a radio frequency cosmetic instrument: randomly selecting 5 volunteers, cleaning the face, and using a radio frequency beauty treatment instrument at the face and the corners of eyes for 20min; 3 times per week for 4 weeks; the average of the corresponding indicators after 4 weeks of the test is recorded. The results are shown in Table 3.

Table 3. Results of anti-aging Performance test of the essences of examples 12-21 and comparative examples 56-73.

As can be seen from Table 3, it can be seen from comparative examples 12 to 21 and comparative examples 56 to 73 that the compounding of antioxidants, compound polypeptides, panthenol and glycoside compounds in specific proportions has a synergistic effect on wrinkle removal in the essence. Meanwhile, the antioxidant, the compound polypeptide, the panthenol and the glycoside compound which are outside the specific proportion have no obvious synergistic effect on repairing and anti-aging of the essence. The antioxidant, the compound polypeptide, the panthenol and the glycoside compounds in a specific proportion are mixed with other moisturizers in the application, and the obtained anti-aging composition also has synergistic wrinkle removal effect consistent with the essence liquid prepared in examples 12-21.

The change of skin wrinkles can be known by matching the comparison mask liquid with the radio frequency beauty instrument, using only the mask liquid and using only the radio frequency beauty instrument: when only the radio frequency beauty instrument is used, the skin wrinkles are slightly reduced, which indicates that the wrinkles can be reduced by using the radio frequency beauty instrument; when only the mask liquid is used, skin wrinkles are slightly reduced; when the mask liquid is matched with a radio frequency beauty treatment instrument for use, the effect of reducing skin wrinkles is greatly increased, so that the cosmetic prepared from the anti-aging composition can promote the regeneration of skin dermis collagen, fill and fade fine lines by matching with the thermal effect of the radio frequency beauty treatment instrument, and achieve the effects of deep anti-aging, long-acting moisturizing, anti-wrinkle and skin nourishing.

Irritation test:

200 skin healthy volunteers aged 18-40 years were selected and randomly split equally into 10 groups of 20. The method comprises the steps of adopting a 48-hour closed patch test method, respectively taking 0.02mL of essence products of examples 12-21 into a patch tester, applying the patch tester to the inner side of the forearm of a subject, using a radio frequency beauty instrument to disperse the essence on the inner side of the forearm of the subject, removing a tested object after 24 hours, keeping the patch part dry, avoiding intense exercise, scratching the patch test part, long-time sunlight irradiation and the like, removing the tester after 48 hours, marking, judging under sufficient light after 30 minutes, and carrying out a return visit observation after 72 hours respectively.

Table 4. Criteria for grading adverse skin reactions.

Test results: the results of the irritation test are shown in Table 5.

Table 5. Results of the irritation test for the essences of examples 12-20.

As can be seen from Table 5, the essences of examples 12-20 of the present application were safe and non-irritating.

Finally, it should be noted that the above-mentioned embodiments are only for illustrating the technical solution of the present application and not for limiting the same, and although the present application has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the specific embodiments of the present application after reading the present specification, and these modifications and variations do not depart from the scope of the application as claimed in the pending claims.

Claims

1. An anti-aging composition is characterized by comprising the following raw materials in parts by mass:

0.1-5 parts of antioxidant;

0.0001-0.05 parts of polypeptide;

1-10 parts of panthenol;

0.3-2 parts of glycoside compound;

the antioxidant is selected from ergothioneine, the polypeptide is selected from acetyl hexapeptide-1, and the glycoside compound is selected from glucopyranoside;

alternatively, the antioxidant is selected from ergothioneine, the polypeptide is selected from tripeptide-1, and the glycoside compound is selected from a combination of dextran and glyceroglycosides;

alternatively, the antioxidant is selected from grape seed extract, the polypeptide is selected from the group consisting of acetyl octapeptide-3 and acetyl hexapeptide-1, and the glycoside compound is selected from the group consisting of glucosides;

alternatively, the antioxidant is selected from ascorbyl ethyl ether, the polypeptide is selected from a combination of acetyl hexapeptide-8 and acetyl hexapeptide-1, and the glycoside compound is selected from glyceroglyceoside;

alternatively, the antioxidant is selected from olive leaf extract, the polypeptide is selected from a combination of acetyl octapeptide-3 and acetyl hexapeptide-1, and the glycoside compound is selected from glyceroglyceoside;

alternatively, the antioxidant is selected from the group consisting of olive leaf extract and glabridin, the polypeptide is selected from pentapeptide-3, and the glycoside compound is selected from the group consisting of glyceroglycosides.

2. The anti-aging composition of claim 1, wherein the anti-aging composition comprises the following raw materials in parts by mass:

1-4 parts of an antioxidant;

0.001-0.03 parts of polypeptide;

3-8 parts of panthenol;

0.5-1.5 parts of glycoside compound;

3. The anti-aging composition of claim 2, wherein the anti-aging composition comprises the following raw materials in parts by mass:

2 parts of an antioxidant;

0.01 part of polypeptide;

5 parts of panthenol;

glycoside compounds 1 part;

4. The anti-aging composition according to claim 1, wherein the process of extracting the olive leaf extract comprises the steps of:

5. The anti-aging composition of claim 1, further comprising 1-20 parts of a polyhydroxy humectant having a carbon chain length of C3-C6.

6. The anti-aging composition of claim 5, further comprising 10 parts of a polyhydroxy humectant having a carbon chain length of C3-C6.

7. The anti-aging composition of claim 6, wherein the polyhydroxy humectant having a carbon chain length of C3-C6 is selected from at least one of glycerol, propylene glycol, butylene glycol, methylpropanediol, rhamnose, erythritol, arabinose.

8. The anti-aging composition of claim 7, wherein the polyhydroxy humectant having a carbon chain length of C3-C6 is selected from at least one of glycerin, rhamnose.

9. The use of an anti-aging composition according to any one of claims 1 to 8 in the preparation of a cosmetic product comprising the above anti-aging composition, wherein the cosmetic product is selected from any one of a pack, an emulsion, a cream, a toner, a serum, a stock solution, a cleanser, a lotion, a perfume, a make-up remover, a foundation, and a concealer.

10. Use of the anti-aging composition according to any one of claims 1 to 8 for the preparation of radiofrequency cosmetic products and medical skin care products.