CN115399265A - Method for hatching artemia cysts - Google Patents
Method for hatching artemia cysts Download PDFInfo
- Publication number
- CN115399265A CN115399265A CN202211064958.8A CN202211064958A CN115399265A CN 115399265 A CN115399265 A CN 115399265A CN 202211064958 A CN202211064958 A CN 202211064958A CN 115399265 A CN115399265 A CN 115399265A
- Authority
- CN
- China
- Prior art keywords
- eggs
- hatching
- artemia cysts
- shelling
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 41
- 208000031513 cyst Diseases 0.000 title claims abstract description 31
- 241001247197 Cephalocarida Species 0.000 title abstract 5
- 238000006703 hydration reaction Methods 0.000 claims abstract description 24
- 239000001963 growth medium Substances 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 230000000813 microbial effect Effects 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 235000013601 eggs Nutrition 0.000 claims description 109
- 238000003756 stirring Methods 0.000 claims description 32
- 239000000243 solution Substances 0.000 claims description 31
- 241000238582 Artemia Species 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- 238000011534 incubation Methods 0.000 claims description 24
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 15
- 239000006249 magnetic particle Substances 0.000 claims description 13
- 241000238426 Anostraca Species 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 11
- 108010015776 Glucose oxidase Proteins 0.000 claims description 11
- 239000004366 Glucose oxidase Substances 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- 229930003268 Vitamin C Natural products 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 11
- 229940116332 glucose oxidase Drugs 0.000 claims description 11
- 235000019420 glucose oxidase Nutrition 0.000 claims description 11
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 11
- 235000013824 polyphenols Nutrition 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- 235000019154 vitamin C Nutrition 0.000 claims description 11
- 239000011718 vitamin C Substances 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 11
- 230000008859 change Effects 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- 239000000645 desinfectant Substances 0.000 claims description 10
- 239000013505 freshwater Substances 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 239000008098 formaldehyde solution Substances 0.000 claims description 9
- 239000012286 potassium permanganate Substances 0.000 claims description 9
- 238000011049 filling Methods 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 4
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 241000588813 Alcaligenes faecalis Species 0.000 claims description 2
- 241000228245 Aspergillus niger Species 0.000 claims description 2
- 241000186660 Lactobacillus Species 0.000 claims description 2
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 claims description 2
- 229940005347 alcaligenes faecalis Drugs 0.000 claims description 2
- 230000000593 degrading effect Effects 0.000 claims description 2
- 229940039696 lactobacillus Drugs 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 229910052763 palladium Inorganic materials 0.000 claims description 2
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 235000012239 silicon dioxide Nutrition 0.000 claims description 2
- 229960003732 tyramine Drugs 0.000 claims description 2
- DZGWFCGJZKJUFP-UHFFFAOYSA-O tyraminium Chemical compound [NH3+]CCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-O 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 claims 1
- 239000007789 gas Substances 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 210000004681 ovum Anatomy 0.000 description 20
- 241000595940 Notostraca Species 0.000 description 13
- 241001122767 Theaceae Species 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 7
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 241000287828 Gallus gallus Species 0.000 description 5
- 238000005273 aeration Methods 0.000 description 5
- 229910001873 dinitrogen Inorganic materials 0.000 description 5
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a method for hatching artemia cysts, which comprises the following steps: s1, hydration reaction, S2 shelling treatment and S3 hatching management, the method for hatching the artemia cysts improves the traditional shelling method, the shelling liquid is matched, a hatching culture medium is reasonably configured, the proportion is scientific so as to promote the efficiency of hatching the artemia cysts, and the bacterial pollution in artemia hatching is reduced by adding a microbial preparation, so that the hatching rate is improved.
Description
Technical Field
The invention relates to the technical field of aquatic products, in particular to a method for hatching artemia cysts.
Background
The brine shrimp egg is also called as brine shrimp, and the egg yolk of the nauplii of Artemia (Artemia) contains more protein and fat, is an essential biological bait in the aquatic breeding process, accounts for 50-70% of the breeding cost, is deeply valued by shrimp nursery, and is widely regarded as an important biological bait. It has strong adaptability to bad environment and has better reproduction ability. The larvae of the fairy shrimp eggs have high nourishment, so the fairy shrimp eggs are excellent biological bait for fish, shrimp and crab. At present, the fairy shrimp egg hatching method in the market can be matched with fairy shrimp cultivation, but in actual use, in the hatching process of fairy shrimp eggs, the problem of how to improve the hatchability of the fairy shrimp eggs is always researched, the method for improving the hatchability by using quantitative fairy shrimp eggs is one of the main modes for reducing the economic investment, the breeding industry has great interest for the method, the existing hatching mode lacks effective pre-selection, and also lacks supervision for the pH value of water quality, so that the hatchability is greatly reduced, and secondly, in the case of the fairy shrimp eggs being piled, the egg body is easy to be piled, the hatching is unfavorable, and the problems exist in practice and need to be solved urgently.
Disclosure of Invention
In view of the above, the present invention provides a method for hatching artemia cysts, which solves the above problems.
The technical scheme of the invention is realized as follows: a method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the eggs into a disinfectant with 20-30 ppm of formaldehyde solution and 10-30 ppm of potassium permanganate solution for hydration reaction for 0.4-1.5 h, and then washing the eggs with water until no peculiar smell exists;
s2, shelling treatment: adding the processed eggs into the shelling liquid, stirring and filling nitrogen, wherein the filling air volume is 10-30 Nm 3 The temperature is set to be 1-3 ℃, stirring is carried out at a constant speed, the color change of eggs is observed within 23-26 ℃, the stirring is stopped when the temperature is from grey white to brown to light yellow and finally to orange, unshelled eggs are screened and collected, and the unshelled eggs are washed clean by fresh water and drained for later use;
s3, incubation management: culturing the eggs in an incubation culture medium, wherein the incubation density of the eggs is 1-3 g/L, the illumination intensity of ultraviolet light is set to be 300-800 Lux, the culture temperature is 22-25 ℃, the pH value is 7.5-8.5, the incubation time is 20-30 h, and the eggs are turned over every 2-4 h, and the turning time is not less than 10min.
Further, the shelling solution is a sodium hydroxide solution with the concentration of 12.5-20.4% by mass fraction.
Further, the uniform stirring speed is 100-200 rpm.
Further, the hatching culture medium comprises the following raw materials in parts by weight: 20 to 30 portions of glucose oxidase, 12 to 26 portions of magnetic particles, 3 to 8 portions of vitamin C, 12 to 20 portions of tea polyphenol, 2 to 8 portions of trehalose and 0.3 to 1 portion of microbial preparation.
Furthermore, the magnetic particles are prepared by fixing nickel or palladium complex on the surface of magnetic silicon dioxide particles by tyramine.
Further, the microbial preparation is any one or combination of bacillus subtilis, lactobacillus, alcaligenes faecalis, aspergillus niger, aniline degrading bacteria and aerobic denitrifying bacteria.
Compared with the prior art, the invention has the beneficial effects that:
the method for hatching the artemia cysts improves the traditional shelling method, firstly, the artemia cysts are subjected to hydration reaction to break the dormancy period, the hatching rate of the artemia cysts is effectively controlled at the initial stage of hatching by the selection requirement of the artemia cysts, the artemia cysts are better shelled under the stimulation of the shelling liquid by matching with the shelling liquid, so that the hatching is facilitated, the hatching management is controlled, the hatching culture medium is reasonably configured, the proportion is scientific so as to promote the hatching efficiency of the artemia cysts, and the bacterial pollution in the artemia hatching is reduced by adding a microbial preparation, so that the hatching rate is improved.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts which have uniform particle size and uniform color and are free from damage or low in breakage rate, wherein most of circular ova are wet ova or empty ova, putting the ova into disinfectant which is prepared by using 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction, reacting for 1.1h, and then using water to wash the ova until no peculiar smell exists;
s2, shelling treatment: adding the processed ovum gallus Domesticus into shelling liquid which is 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with aeration amount of 20Nm 3 At 2 deg.C, stirring at 150rpm, and 24 deg.C, observing the color change of insect egg from grey whiteStopping stirring until brown to light yellow and finally orange, sieving, collecting shelled eggs, washing with fresh water, and draining;
s3, incubation management: putting the strain into an incubation culture medium for culturing, wherein the incubation culture medium comprises the following raw materials in parts by weight: 20 parts of glucose oxidase, 12 parts of magnetic particles, 3 parts of vitamin C, 12 parts of tea polyphenol, 2 parts of trehalose and 0.3 part of bacillus subtilis, wherein the hatching density of the eggs is 2g/L, the illumination intensity of ultraviolet light is set to be 500Lux, the culture temperature is 23 ℃, the pH value is 8.0, the hatching time is 25h, the eggs are turned over every 3h, and the turning time is not less than 10min.
Example 2
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts which have uniform particle size and uniform color and are free from damage or low in breakage rate, wherein most of circular ova are wet ova or empty ova, putting the ova into disinfectant which is prepared by using 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction, reacting for 1.1h, and then using water to wash the ova until no peculiar smell exists;
s2, shelling treatment: adding the processed ovum gallus Domesticus into shelling solution (sodium hydroxide solution with concentration of 14.3 wt%), stirring, and introducing nitrogen gas with aeration amount of 20Nm 3 H, setting the temperature to be 2 ℃, stirring at a constant speed of 150rpm, observing the color change of eggs at the temperature of 24 ℃, stopping stirring when the color is changed from grey white to brown to light yellow and finally to orange, sieving, collecting unshelled eggs, washing with fresh water, and draining for later use;
s3, hatching management: putting the strain into an incubation culture medium for culturing, wherein the incubation culture medium comprises the following raw materials in parts by weight: 30 parts of glucose oxidase, 26 parts of magnetic particles, 8 parts of vitamin C, 20 parts of tea polyphenol, 8 parts of trehalose and 1 part of bacillus subtilis, wherein the hatching density of eggs is 2g/L, the illumination intensity of ultraviolet light is set to be 500Lux, the culture temperature is 23 ℃, the pH value is 8.0, the hatching time is 25h, the eggs are turned over every 3h, and the turning time is not less than 10min.
Example 3
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts which have uniform particle size and uniform color and are free from damage or low in breakage rate, wherein most of circular ova are wet ova or empty ova, putting the ova into disinfectant which is prepared by using 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction, reacting for 1.1h, and then using water to wash the ova until no peculiar smell exists;
s2, shelling treatment: adding the processed ovum gallus Domesticus into shelling liquid which is 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with aeration amount of 20Nm 3 The temperature is set to be 2 ℃, the uniform stirring speed is 150rpm, the color change of the worm eggs is observed within 24 ℃, the stirring is stopped from the gray color to the brown color to the light yellow color and finally to the orange color, the unshelled eggs are screened and collected, and the unshelled eggs are washed clean by fresh water and drained for later use;
s3, hatching management: putting the strain into a hatching culture medium for culturing, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of the eggs is 2g/L, the illumination intensity of ultraviolet light is set to be 500Lux, the culture temperature is 23 ℃, the pH value is 8.0, the hatching time is 25h, the eggs are turned over every 3h, and the turning time is not less than 10min.
Example 4
A method for hatching fairy shrimp eggs comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the eggs into a disinfectant solution prepared from 20ppm of formaldehyde solution and 10ppm of potassium permanganate solution for hydration reaction for 0.4h, and then flushing the eggs with water until no peculiar smell exists;
s2, shelling treatment: adding the processed ovum gallus Domesticus into shelling liquid which is 12.5% sodium hydroxide solution, stirring, introducing nitrogen gas,the aeration rate is 10Nm 3 The temperature is set to be 1 ℃, the stirring is carried out at a constant speed of 100pm, the color change of eggs is observed at the temperature of 23 ℃, the stirring is stopped from gray color to brown color to light yellow color and finally to orange color, the unshelled eggs are screened and collected, and the unshelled eggs are washed clean by fresh water and drained for later use;
s3, hatching management: putting the strain into a hatching culture medium for culturing, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of the eggs is 1g/L, the illumination intensity of ultraviolet light is set to be 300Lux, the culture temperature is 22 ℃, the pH value is 7.5, the hatching time is 20 hours, the eggs are turned over every 2 hours, and the turning time is not less than 10 minutes.
Example 5
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the eggs into a disinfectant solution prepared from 30ppm of formaldehyde solution and 30ppm of potassium permanganate solution for hydration reaction for 1.5 hours, and then flushing the eggs with water until no peculiar smell exists;
s2, shelling treatment: adding the processed eggs into a shelling solution, wherein the shelling solution is a sodium hydroxide solution with the concentration of 20.4 percent by mass, stirring and filling nitrogen, the air inflation amount is 30Nm & lt 3 & gt/h, the temperature is set to be 3 ℃, the uniform stirring speed is 200rpm, the temperature is within 26 ℃, observing the color change of the eggs, stopping stirring when the temperature is from grey white to brown to light yellow and finally to orange, sieving and collecting the shelled eggs, washing the shelled eggs with fresh water, and draining for later use;
s3, hatching management: putting the strain into an incubation culture medium for culturing, wherein the incubation culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of eggs is 3g/L, the illumination intensity of ultraviolet light is set to be 800Lux, the culture temperature is 25 ℃, the pH value is 8.5, the hatching time is 30h, the eggs are turned over every 4h, and the turning time is not less than 10min.
Comparative example 1
The difference between the comparative example and the example 3 is that the hydration reaction is carried out by putting the artemia cysts into saline water with the salinity of 1 percent, in particular to a method for hatching the artemia cysts, which comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the brine shrimp eggs into saline water with the salinity of 1% for hydration reaction for 1.1h, and then washing the brine shrimp eggs until the brine shrimp eggs have no peculiar smell;
s2, shelling treatment: adding the processed eggs into a shelling solution, wherein the shelling solution is a sodium hydroxide solution with the concentration of 14.3 percent by mass, stirring and filling nitrogen, the air inflation amount is 20Nm & lt 3 & gt/h, the temperature is set to be 2 ℃, the uniform stirring speed is 150rpm, the temperature is within 24 ℃, observing the color change of the eggs, stopping stirring when the temperature is from grey white to brown to light yellow and finally to orange, sieving and collecting the shelled eggs, washing the shelled eggs with fresh water, and draining for later use;
s3, hatching management: putting the strain into an incubation culture medium for culturing, wherein the incubation culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of the eggs is 2g/L, the illumination intensity of ultraviolet light is set to be 500Lux, the culture temperature is 23 ℃, the pH value is 8.0, the hatching time is 25h, the eggs are turned over every 3h, and the turning time is not less than 10min
Comparative example 2
The difference between the comparative example and the example 3 is that the incubation medium does not contain magnetic particles, in particular to a method for incubating artemia cysts: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the eggs into a disinfectant solution prepared from 25ppm of a formaldehyde solution and 20ppm of a potassium permanganate solution for hydration reaction for 1.1h, and then washing the eggs with water until no peculiar smell exists;
s2, shelling treatment: adding the processed ovum gallus Domesticus into shelling liquid which is 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with aeration amount of 20Nm 3 The temperature is set to be 2 ℃, the uniform stirring speed is 150rpm, the color change of the worm eggs is observed within 24 ℃, the stirring is stopped from the gray color to the brown color to the light yellow color and finally to the orange color, the unshelled eggs are screened and collected, and the unshelled eggs are washed clean by fresh water and drained for later use;
s3, hatching management: putting the strain into a hatching culture medium for culturing, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of the eggs is 2g/L, the illumination intensity of ultraviolet light is set to be 500Lux, the culture temperature is 23 ℃, the pH value is 8.0, the hatching time is 25 hours, the eggs are turned over every 3 hours, and the turning time is not less than 10min.
Comparative example 3
The comparative example differs from example 3 in that the incubation medium comprises the following raw materials in parts by weight: 10 parts of glucose oxidase, 10 parts of magnetic particles, 10 parts of vitamin C, 10 parts of tea polyphenol, 1 part of trehalose and 0.1 part of microbial preparation; in particular to a method for hatching artemia cysts, which comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the eggs into a disinfectant solution prepared from 25ppm of a formaldehyde solution and 20ppm of a potassium permanganate solution for hydration reaction for 1.1h, and then washing the eggs with water until no peculiar smell exists;
s2, shelling treatment: adding the processed eggs into a shelling solution, wherein the shelling solution is a sodium hydroxide solution with the concentration of 14.3 percent by mass, stirring and filling nitrogen, the air inflation amount is 20Nm & lt 3 & gt/h, the temperature is set to be 2 ℃, the uniform stirring speed is 150rpm, the temperature is within 24 ℃, observing the color change of the eggs, stopping stirring when the temperature is from grey white to brown to light yellow and finally to orange, sieving and collecting the shelled eggs, washing the shelled eggs with fresh water, and draining for later use;
s3, hatching management: putting the strain into an incubation culture medium for culturing, wherein the incubation culture medium comprises the following raw materials in parts by weight: 10 parts of glucose oxidase, 10 parts of magnetic particles, 10 parts of vitamin C, 10 parts of tea polyphenol, 1 part of trehalose and 0.1 part of microbial agent, wherein the hatching density of the eggs is 2g/L, the illumination intensity of ultraviolet light is set to be 500Lux, the culture temperature is 23 ℃, the pH value is 8.0, the hatching time is 25h, the eggs are turned over every 3h, and the turning time is not less than 10min.
The fairy shrimp eggs were incubated according to the incubation methods of the above examples 1 to 5 and comparative examples 1 to 2, at an incubation site at the base of fishing bay at wen hitian gulf, wenchang city, hainan province, for 2 months, and the incubated egg eggs were calculated according to a density method, and the shelling condition and the incubation rate were observed during the period, wherein the incubation rate was according to the following formula:
hatchability = hatching eggs/total hatch × 100%
According to the results, the method for hatching the fairy shrimp eggs effectively controls the hatching rate of the fairy shrimp eggs at the initial stage of hatching by selecting the fairy shrimp eggs, adds the disinfectant to carry out hydration reaction on the eggs, and leads the eggs to be well shelled under the stimulation of the shelling liquid by matching with the shelling liquid, thereby being beneficial to hatching.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.
Claims (6)
1. A method for hatching artemia cysts is characterized in that: the method comprises the following steps:
s1, hydration reaction: selecting high-quality brine shrimp eggs which are uniform in particle size, uniform in color, free of damage or low in breakage rate, mostly wet eggs or empty eggs, putting the eggs into a disinfectant with 20-30 ppm of formaldehyde solution and 10-30 ppm of potassium permanganate solution for hydration reaction for 0.4-1.5 h, and then washing the eggs with water until no peculiar smell exists;
s2, shelling treatment: adding the processed eggs into shelling liquid, stirring and filling nitrogen, wherein the filling gas amount is 10-30 Nm & lt 3 & gt/h, the temperature is set to be 1-3 ℃, stirring at a constant speed, the temperature is 23-26 ℃, observing the color change of the eggs, stopping stirring from gray, brown, light yellow and orange, sieving and collecting the shelled eggs, washing with fresh water, draining and reserving for later use;
s3, hatching management: culturing the eggs in an incubation culture medium, wherein the incubation density of the eggs is 1-3 g/L, the illumination intensity of ultraviolet light is set to be 300-800 Lux, the culture temperature is 22-25 ℃, the pH value is 7.5-8.5, the incubation time is 20-30 h, and the eggs are turned over every 2-4 h, and the turning time is not less than 10min.
2. The method for hatching the artemia cysts according to claim 1, wherein: the shelling liquid is sodium hydroxide solution with the concentration of 12.5-20.4% by mass.
3. The method for hatching the artemia cysts according to claim 1, wherein: the uniform stirring speed is 100-200 rpm.
4. The method for hatching the artemia cysts according to claim 1, wherein: the hatching culture medium comprises the following raw materials in parts by weight: 20 to 30 portions of glucose oxidase, 12 to 26 portions of magnetic particles, 3 to 8 portions of vitamin C, 12 to 20 portions of tea polyphenol, 2 to 8 portions of trehalose and 0.3 to 1 portion of microbial preparation.
5. The method for hatching the artemia cysts according to claim 4, wherein: the magnetic particles are prepared by fixing nickel or palladium complex on the surface of magnetic silicon dioxide particles by tyramine.
6. The method for hatching the artemia cysts according to claim 4, wherein: the microbial preparation is any one or a combination of bacillus subtilis, lactobacillus, alcaligenes faecalis, aspergillus niger, aniline degrading bacteria and aerobic denitrifying bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211064958.8A CN115399265B (en) | 2022-09-01 | 2022-09-01 | Hatching method for artemia cysts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211064958.8A CN115399265B (en) | 2022-09-01 | 2022-09-01 | Hatching method for artemia cysts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115399265A true CN115399265A (en) | 2022-11-29 |
| CN115399265B CN115399265B (en) | 2024-01-26 |
Family
ID=84164406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211064958.8A Active CN115399265B (en) | 2022-09-01 | 2022-09-01 | Hatching method for artemia cysts |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115399265B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532579A (en) * | 2015-12-08 | 2016-05-04 | 厦门金益海生物科技有限公司 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
| CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
| CN107535434A (en) * | 2016-06-29 | 2018-01-05 | 绿奥环保科技（上海）有限公司 | A kind of nuisanceless brine shrimp eggs hatching method |
| CN109122446A (en) * | 2018-09-01 | 2019-01-04 | 周玉兰 | A kind of processing method improving brine shrimp eggs hatching rate |
| CN112645994A (en) * | 2021-01-14 | 2021-04-13 | 湖州柳荫生物科技有限公司 | Extraction process of salidroside |
-
2022
- 2022-09-01 CN CN202211064958.8A patent/CN115399265B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532579A (en) * | 2015-12-08 | 2016-05-04 | 厦门金益海生物科技有限公司 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
| CN107535434A (en) * | 2016-06-29 | 2018-01-05 | 绿奥环保科技（上海）有限公司 | A kind of nuisanceless brine shrimp eggs hatching method |
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
| CN109122446A (en) * | 2018-09-01 | 2019-01-04 | 周玉兰 | A kind of processing method improving brine shrimp eggs hatching rate |
| CN112645994A (en) * | 2021-01-14 | 2021-04-13 | 湖州柳荫生物科技有限公司 | Extraction process of salidroside |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115399265B (en) | 2024-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6267810B2 (en) | Oyster farming method | |
| CN102754610B (en) | Method for improving hippocampus kelloggi fry cultivating rate | |
| CN111713438A (en) | Method for cultivating high-quality and high-yield penaeus vannamei larvae | |
| CN110973019B (en) | Method for promoting survival and growth of takifugu rubripes from fertilized eggs to larvae and juvenile fish by using LED light source | |
| JP2006507826A (en) | Microbial feed for filtered ingested aqueous organisms | |
| CN110679524A (en) | Indoor industrialized ecological specific pathogen-free seedling cultivation method for litopenaeus vannamei | |
| CN111226843A (en) | Special artemia indoor culture method for seawater fish and shrimp seedling culture | |
| CN109122443A (en) | Australia freshwater lobster recirculated water oogenesis hatching system and method for culturing seedlings | |
| CN114766397B (en) | Cultivation method of bellied sea horse | |
| CN103314895A (en) | Artificial breeding method for parapenaeopsis hardwickii | |
| CN111758617A (en) | Indoor cement pond artificial breeding method for takifugu obscurus | |
| CN107410757B (en) | Preparation method of composite liquid bait for artificial coral culture | |
| JPS58175451A (en) | Artificial raising method of lobster and crab | |
| CN108094283A (en) | A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling | |
| Southgate et al. | Hatchery and larval foods | |
| CN111771772A (en) | Grouper fry breeding method | |
| CN104872013A (en) | Garrupa farming method and system | |
| CN113080110B (en) | Method for promoting gonad maturation of parent fish of spotted-head fish | |
| CN1363212A (en) | Low-salinity artificial brooding and fresh-water culturing method for fugus | |
| CN117178921A (en) | Multi-stage ecological polyculture method for Babylonia | |
| CN115399265B (en) | Hatching method for artemia cysts | |
| CN1312988C (en) | Nanjing fairy shrimp egg proliferating method | |
| CN109133356B (en) | Method for removing algae by using filter feeders | |
| RU2614644C1 (en) | Method of intensive cohort cultivation of acartia (sea calanoid copepods) | |
| RU2670159C1 (en) | Method of long-term storage of eggs of calanidic copepods of acartias for receiving synchronous culture of same age nauplii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |