CN115399265B - Hatching method for artemia cysts - Google Patents
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- CN115399265B CN115399265B CN202211064958.8A CN202211064958A CN115399265B CN 115399265 B CN115399265 B CN 115399265B CN 202211064958 A CN202211064958 A CN 202211064958A CN 115399265 B CN115399265 B CN 115399265B
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- 208000031513 cyst Diseases 0.000 title claims abstract description 108
- 230000012447 hatching Effects 0.000 title claims abstract description 98
- 238000000034 method Methods 0.000 title claims abstract description 40
- 241001247197 Cephalocarida Species 0.000 title claims abstract 12
- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 230000000813 microbial effect Effects 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 235000013601 eggs Nutrition 0.000 claims description 54
- 238000003756 stirring Methods 0.000 claims description 32
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- 238000006703 hydration reaction Methods 0.000 claims description 23
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 15
- 239000006249 magnetic particle Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 11
- 108010015776 Glucose oxidase Proteins 0.000 claims description 11
- 239000004366 Glucose oxidase Substances 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- 229930003268 Vitamin C Natural products 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 11
- 229940116332 glucose oxidase Drugs 0.000 claims description 11
- 235000019420 glucose oxidase Nutrition 0.000 claims description 11
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 11
- 235000013824 polyphenols Nutrition 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- 235000019154 vitamin C Nutrition 0.000 claims description 11
- 239000011718 vitamin C Substances 0.000 claims description 11
- 230000008859 change Effects 0.000 claims description 10
- 239000000645 desinfectant Substances 0.000 claims description 10
- 238000011010 flushing procedure Methods 0.000 claims description 10
- 239000013505 freshwater Substances 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 239000008098 formaldehyde solution Substances 0.000 claims description 9
- 239000012286 potassium permanganate Substances 0.000 claims description 9
- 238000007873 sieving Methods 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 210000004681 ovum Anatomy 0.000 claims description 5
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 4
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 241000588813 Alcaligenes faecalis Species 0.000 claims description 2
- 241000228245 Aspergillus niger Species 0.000 claims description 2
- 241000186660 Lactobacillus Species 0.000 claims description 2
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 claims description 2
- 229940005347 alcaligenes faecalis Drugs 0.000 claims description 2
- 230000015556 catabolic process Effects 0.000 claims description 2
- 238000006731 degradation reaction Methods 0.000 claims description 2
- 230000003100 immobilizing effect Effects 0.000 claims description 2
- 229940039696 lactobacillus Drugs 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 229910052763 palladium Inorganic materials 0.000 claims description 2
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 235000012239 silicon dioxide Nutrition 0.000 claims description 2
- 229960003732 tyramine Drugs 0.000 claims description 2
- DZGWFCGJZKJUFP-UHFFFAOYSA-O tyraminium Chemical compound [NH3+]CCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-O 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 241000238582 Artemia Species 0.000 description 71
- 239000000243 solution Substances 0.000 description 25
- 241001122767 Theaceae Species 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 7
- 238000005406 washing Methods 0.000 description 6
- 229910001873 dinitrogen Inorganic materials 0.000 description 5
- 241000238557 Decapoda Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 241001672739 Artemia salina Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238426 Anostraca Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a method for hatching artemia cysts, which comprises the following steps: the method for hatching the artemia cysts improves the traditional shelling method, reasonably configures the hatching culture medium by matching with the shelling liquid, and scientifically mixes the shelling liquid to promote the hatching efficiency of the artemia cysts, and reduces bacterial pollution in artemia hatching by adding a microbial preparation, thereby improving the hatching rate.
Description
Technical Field
The invention relates to the technical field of aquatic products, in particular to a method for hatching artemia cysts.
Background
Artemia eggs are also called brine shrimp, artemia (Artemia) nauplii yolk contains more protein and fat, is an essential biological bait in the aquatic breeding process, accounts for 50-70% of the breeding cost, is deeply valued by shrimp plantlets, and is widely regarded as an important biological bait. It has strong adaptability to bad environment and good reproduction ability. The artemia cysts have high nutrition and are therefore excellent biological baits for fish, shrimp and crabs. At present, the method for hatching the artemia cysts on the market can be matched with the cultivation of the artemia cysts, but in the actual use, how to improve the hatching rate of the artemia cysts in the hatching process of the artemia cysts is a problem which is always researched, the method for improving the hatching rate by using quantitative artemia cysts is one of the main ways for reducing the economic investment, the breeding industry has great interest on the method, the existing hatching ways have few effective pre-selection, the pH value of water quality also has no supervision, the hatching rate is greatly reduced, and egg accumulation is easy to cause under the condition of the accumulation of the artemia cysts, and the method is unfavorable for hatching, which are the problems which are actually existing and need to be solved urgently.
Disclosure of Invention
In view of the above, the present invention proposes a method for hatching artemia cysts, which solves the above-mentioned problems.
The technical scheme of the invention is realized as follows: a method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size, uniform in color, free of damage or low in damage rate, round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 20-30 ppm of formaldehyde solution and 10-30 ppm of potassium permanganate solution for hydration reaction for 0.4-1.5 hours, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution into the processed ovum, stirring, and charging nitrogen with the charging amount of 10-30 Nm 3 Stirring at constant speed at the temperature of 1-3 ℃ and at the temperature of 23-26 DEG CObserving color change of the eggs within a certain range, stopping stirring from gray to brown to light yellow and finally to orange, sieving, collecting shelled eggs, washing with fresh water, and draining for later use;
s3, hatching management: culturing the eggs in a hatching medium, wherein the hatching density of the eggs is 1-3 g/L, the illumination intensity of ultraviolet light is 300-800 Lux, the culturing temperature is 22-25 ℃, the pH is 7.5-8.5, the hatching time is 20-30 h, and the eggs are turned over every 2-4 h, and the turning over time is not less than 10min.
Further, the shelling liquid is sodium hydroxide solution with the concentration of 12.5-20.4% by mass.
Further, the uniform stirring speed is 100-200 rpm.
Further, the hatching medium comprises the following raw materials in parts by weight: 20-30 parts of glucose oxidase, 12-26 parts of magnetic particles, 3-8 parts of vitamin C, 12-20 parts of tea polyphenol, 2-8 parts of trehalose and 0.3-1 part of microbial preparation.
Further, the magnetic particles are prepared by immobilizing nickel or palladium complex on the surfaces of magnetic silicon dioxide material particles through tyramine.
Further, the microbial preparation is any one or a combination of a plurality of bacillus subtilis, lactobacillus, alcaligenes faecalis, aspergillus niger, aniline degradation bacteria and aerobic denitrifying bacteria.
Compared with the prior art, the invention has the beneficial effects that:
the method for hatching the artemia cysts improves the traditional shelling method, firstly, the artemia cysts are subjected to hydration reaction to break the dormancy period, the hatching rate of the artemia cysts is effectively controlled at the initial stage of hatching by the selection requirement of the artemia cysts, and the shelling liquid is matched with the shelling liquid, so that the artemia cysts are better shelled under the stimulation of the shelling liquid, the hatching is facilitated, the hatching management is controlled, the hatching culture medium is reasonably configured, the proportion is scientific to promote the hatching efficiency of the artemia cysts, and bacterial pollution in the artemia hatching is reduced by adding a microbial preparation, so that the hatching rate is improved.
Detailed Description
In order to better understand the technical content of the present invention, the following provides specific examples to further illustrate the present invention.
The experimental methods used in the embodiment of the invention are conventional methods unless otherwise specified.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction for 1.1h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution of 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with charging amount of 20Nm 3 And (3) stirring at a constant speed of 150rpm at a temperature of 2 ℃, observing the color change of the eggs at a temperature of 24 ℃, stopping stirring from gray to brown to light yellow and finally to orange, sieving, collecting shelled eggs, washing fresh water, and draining for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 20 parts of glucose oxidase, 12 parts of magnetic particles, 3 parts of vitamin C, 12 parts of tea polyphenol, 2 parts of trehalose and 0.3 part of bacillus subtilis, wherein the hatching density of eggs is 2g/L, the illumination intensity of ultraviolet light is 500Lux, the culture temperature is 23 ℃, the pH is 8.0, the hatching time is 25 hours, and the eggs are turned over every 3 hours for not less than 10 minutes.
Example 2
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction for 1.1h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution of 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with charging amount of 20Nm 3 And (3) stirring at a constant speed of 150rpm at a temperature of 2 ℃, observing the color change of the eggs at a temperature of 24 ℃, stopping stirring from gray to brown to light yellow and finally to orange, sieving, collecting shelled eggs, washing fresh water, and draining for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 30 parts of glucose oxidase, 26 parts of magnetic particles, 8 parts of vitamin C, 20 parts of tea polyphenol, 8 parts of trehalose and 1 part of bacillus subtilis, wherein the hatching density of eggs is 2g/L, the illumination intensity of ultraviolet light is 500Lux, the culture temperature is 23 ℃, the pH is 8.0, the hatching time is 25h, and the eggs are turned over every 3h for not less than 10min.
Example 3
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction for 1.1h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution of 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with charging amount of 20Nm 3 And/h, setting the temperature to 2 ℃, stirring at a constant speed of 150rpm, observing the color change of the ova at the temperature of 24 ℃, stopping stirring from off-white to brown to light yellow and finally to orange,sieving to collect shelled eggs, washing with fresh water, and draining for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of eggs is 2g/L, the illumination intensity of ultraviolet light is 500Lux, the culture temperature is 23 ℃, the pH is 8.0, the hatching time is 25 hours, and the eggs are turned over every 3 hours for not less than 10 minutes.
Example 4
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 20ppm of formaldehyde solution and 10ppm of potassium permanganate solution for hydration reaction for 0.4h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution of 12.5% sodium hydroxide solution into the processed ovum, stirring, and charging nitrogen gas with an aeration rate of 10Nm 3 And (3) stirring at a constant speed of 100pm at a temperature of 1 ℃, observing the color change of the eggs at a temperature of 23 ℃, stopping stirring from gray to brown to light yellow and finally to orange, sieving, collecting shelled eggs, washing fresh water, and draining for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of the eggs is 1g/L, the illumination intensity of ultraviolet light is 300Lux, the culture temperature is 22 ℃, the pH is 7.5, the hatching time is 20 hours, and the eggs are turned over every 2 hours for not less than 10 minutes.
Example 5
A method for hatching artemia cysts comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 30ppm of formaldehyde solution and 30ppm of potassium permanganate solution for hydration reaction for 1.5 hours, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution into the processed eggs, wherein the shelling solution is sodium hydroxide solution with the concentration of 20.4% by mass, stirring and charging nitrogen, the charging amount is 30Nm3/h, the temperature is set to 3 ℃, the speed is 200rpm, stirring is uniform, the temperature is within 26 ℃, the color change of the eggs is observed, stirring is stopped when the color of the eggs is from grey to brown to light yellow, and finally to orange, the shelling eggs are sieved and collected, fresh water is washed cleanly, and the eggs are drained for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of eggs is 3g/L, the illumination intensity of ultraviolet light is 800Lux, the culture temperature is 25 ℃, the pH is 8.5, the hatching time is 30 hours, and the eggs are turned over every 4 hours for not less than 10 minutes.
Comparative example 1
The difference between the comparative example and the example 3 is that the hydration reaction is carried out by putting the artemia salina eggs into saline water with the salinity of 1 percent, and the method is specifically that the incubation method of the artemia salina eggs comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into saline water with the salinity of 1% for hydration reaction for 1.1h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding the treated eggs into shelling liquid, namely a sodium hydroxide solution with the concentration of 14.3% by mass, stirring and charging nitrogen, wherein the charging amount is 20Nm3/h, the temperature is set to be 2 ℃, the speed is 150rpm, stirring is carried out at a constant speed, the temperature is within 24 ℃, the color change of the eggs is observed, stirring is stopped when the color of the eggs is changed from grey to brown to light yellow, and finally to orange, the shelling eggs are sieved and collected, fresh water is washed cleanly, and the eggs are drained for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 20 parts of magnetic particles, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of eggs is 2g/L, the illumination intensity of ultraviolet light is 500Lux, the culture temperature is 23 ℃, the pH is 8.0, the hatching time is 25 hours, the eggs are turned over every 3 hours, and the turning-over time is not less than 10 minutes
Comparative example 2
The difference between this comparative example and example 3 is that the hatching medium does not contain magnetic particles, and specifically is a method for hatching artemia cysts: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction for 1.1h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution of 14.3% sodium hydroxide solution, stirring, and charging nitrogen gas with charging amount of 20Nm 3 And (3) stirring at a constant speed of 150rpm at a temperature of 2 ℃, observing the color change of the eggs at a temperature of 24 ℃, stopping stirring from gray to brown to light yellow and finally to orange, sieving, collecting shelled eggs, washing fresh water, and draining for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 25 parts of glucose oxidase, 5 parts of vitamin C, 16 parts of tea polyphenol, 6 parts of trehalose and 0.7 part of bacillus subtilis, wherein the hatching density of the eggs is 2g/L, the illumination intensity of ultraviolet light is 500Lux, the culture temperature is 23 ℃, the pH is 8.0, the hatching time is 25 hours, the eggs are turned over every 3 hours, and the turning-over time is not less than 10 minutes.
Comparative example 3
The difference between this comparative example and example 3 is that the hatching medium comprises the following raw materials in parts by weight: 10 parts of glucose oxidase, 10 parts of magnetic particles, 10 parts of vitamin C, 10 parts of tea polyphenol, 1 part of trehalose and 0.1 part of microbial preparation; in particular to a method for hatching artemia cysts, which comprises the following steps: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size and color, free of damage or low in damage rate, the round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 25ppm of formaldehyde solution and 20ppm of potassium permanganate solution for hydration reaction for 1.1h, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding the treated eggs into shelling liquid, namely a sodium hydroxide solution with the concentration of 14.3% by mass, stirring and charging nitrogen, wherein the charging amount is 20Nm3/h, the temperature is set to be 2 ℃, the speed is 150rpm, stirring is carried out at a constant speed, the temperature is within 24 ℃, the color change of the eggs is observed, stirring is stopped when the color of the eggs is changed from grey to brown to light yellow, and finally to orange, the shelling eggs are sieved and collected, fresh water is washed cleanly, and the eggs are drained for later use;
s3, hatching management: placing the strain into a hatching culture medium for culture, wherein the hatching culture medium comprises the following raw materials in parts by weight: 10 parts of glucose oxidase, 10 parts of magnetic particles, 10 parts of vitamin C, 10 parts of tea polyphenol, 1 part of trehalose and 0.1 part of microbial preparation, wherein the hatching density of eggs is 2g/L, the illumination intensity of ultraviolet light is 500Lux, the culture temperature is 23 ℃, the pH is 8.0, the hatching time is 25h, and the eggs are turned over every 3h, and the turning-over time is not less than 10min.
The hatching method of examples 1 to 5 and comparative examples 1 to 2 was carried out on artemia cysts, the hatching address was set at the bay base of the bay of Wen Zhenfeng of Wenchang City, hainan province, the test time was 2 months, the hatched artemia cysts were calculated according to the density method, and the shelling condition and the hatching rate in the observation period were calculated according to the following formula:
hatchability = hatching eggs/total hatching number x 100%
From the results, the method for hatching the artemia cysts effectively controls the hatching rate of the artemia cysts at the initial stage of hatching by selecting the artemia cysts, adds disinfectant to carry out hydration reaction on the artemia cysts, and combines the shelling liquid of the invention to better shell the artemia cysts under the stimulation of the shelling liquid, thereby being beneficial to hatching.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (3)
1. A method for hatching artemia cysts is characterized in that: the method comprises the following steps:
s1, hydration reaction: selecting high-quality artemia cysts, wherein the artemia cysts are uniform in particle size, uniform in color, free of damage or low in damage rate, round cysts are wet cysts or empty cysts, putting the artemia cysts into a disinfectant with 20-30 ppm of formaldehyde solution and 10-30 ppm of potassium permanganate solution for hydration reaction for 0.4-1.5 hours, and then flushing the artemia cysts with water until no peculiar smell exists;
s2, shelling: adding shelling solution into the processed ovum, stirring, and charging nitrogen with the charging amount of 10-30 Nm 3 And/h, setting the temperature to be 1-3 ℃, stirring at a constant speed, observing the color change of the ova at the temperature of 23-26 ℃, stopping stirring from gray to brown to light yellow and finally to orange, sieving and collecting the shellingWashing eggs with fresh water, and draining for later use;
s3, hatching management: culturing the eggs in a hatching medium, wherein the hatching density of the eggs is 1-3 g/L, the illumination intensity of ultraviolet light is 300-800 Lux, the culturing temperature is 22-25 ℃, the pH is 7.5-8.5, the hatching time is 20-30 h, and the eggs are turned over every 2-4 h, and the turning over time is not less than 10min;
the uniform stirring speed is 100-200 rpm;
the hatching culture medium comprises the following raw materials in parts by weight: 20-30 parts of glucose oxidase, 12-26 parts of magnetic particles, 3-8 parts of vitamin C, 12-20 parts of tea polyphenol, 2-8 parts of trehalose and 0.3-1 part of microbial preparation;
the microbial preparation is any one or a combination of a plurality of bacillus subtilis, lactobacillus, alcaligenes faecalis, aspergillus niger, aniline degradation bacteria and aerobic denitrifying bacteria.
2. A method of hatching artemia cysts according to claim 1 characterised in that: the shelling liquid is sodium hydroxide solution with the concentration of 12.5-20.4% by mass.
3. A method of hatching artemia cysts according to claim 1 characterised in that: the magnetic particles are prepared by immobilizing nickel or palladium complex on the surfaces of magnetic silicon dioxide particles by tyramine.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532579A (en) * | 2015-12-08 | 2016-05-04 | 厦门金益海生物科技有限公司 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
| CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
| CN107535434A (en) * | 2016-06-29 | 2018-01-05 | 绿奥环保科技（上海）有限公司 | A kind of nuisanceless brine shrimp eggs hatching method |
| CN109122446A (en) * | 2018-09-01 | 2019-01-04 | 周玉兰 | A kind of processing method improving brine shrimp eggs hatching rate |
| CN112645994A (en) * | 2021-01-14 | 2021-04-13 | 湖州柳荫生物科技有限公司 | Extraction process of salidroside |
-
2022
- 2022-09-01 CN CN202211064958.8A patent/CN115399265B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532579A (en) * | 2015-12-08 | 2016-05-04 | 厦门金益海生物科技有限公司 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
| CN107535434A (en) * | 2016-06-29 | 2018-01-05 | 绿奥环保科技（上海）有限公司 | A kind of nuisanceless brine shrimp eggs hatching method |
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
| CN109122446A (en) * | 2018-09-01 | 2019-01-04 | 周玉兰 | A kind of processing method improving brine shrimp eggs hatching rate |
| CN112645994A (en) * | 2021-01-14 | 2021-04-13 | 湖州柳荫生物科技有限公司 | Extraction process of salidroside |
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