CN105532579A - Hatching culture medium of artemia cyst, and preparation method and application thereof - Google Patents
Hatching culture medium of artemia cyst, and preparation method and application thereof Download PDFInfo
- Publication number
- CN105532579A CN105532579A CN201510894932.XA CN201510894932A CN105532579A CN 105532579 A CN105532579 A CN 105532579A CN 201510894932 A CN201510894932 A CN 201510894932A CN 105532579 A CN105532579 A CN 105532579A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- parts
- brine shrimp
- shrimp eggs
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000001963 growth medium Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 230000012447 hatching Effects 0.000 title abstract description 19
- 208000031513 cyst Diseases 0.000 title abstract 3
- 241001247197 Cephalocarida Species 0.000 title 1
- 206010011732 Cyst Diseases 0.000 title 1
- 241000238426 Anostraca Species 0.000 claims abstract description 53
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000011780 sodium chloride Substances 0.000 claims abstract description 13
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 11
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 11
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 10
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 10
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 10
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- 239000008103 glucose Substances 0.000 claims abstract description 9
- 235000013601 eggs Nutrition 0.000 claims description 55
- 239000002609 medium Substances 0.000 claims description 31
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 claims description 30
- 238000011534 incubation Methods 0.000 claims description 28
- 239000002994 raw material Substances 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 101100496858 Mus musculus Colec12 gene Proteins 0.000 claims description 10
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 claims description 9
- 235000015424 sodium Nutrition 0.000 claims description 9
- 239000011782 vitamin Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical group C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 239000013049 sediment Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000003002 pH adjusting agent Substances 0.000 claims description 5
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 239000001630 malic acid Substances 0.000 claims description 2
- 235000011090 malic acid Nutrition 0.000 claims description 2
- 239000001509 sodium citrate Substances 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
- 235000011083 sodium citrates Nutrition 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract description 7
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 244000144974 aquaculture Species 0.000 abstract description 4
- 241000238582 Artemia Species 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract 1
- 241001122767 Theaceae Species 0.000 abstract 1
- 229930003268 Vitamin C Natural products 0.000 abstract 1
- 230000000249 desinfective effect Effects 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- 229910001631 strontium chloride Inorganic materials 0.000 abstract 1
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 abstract 1
- 235000019154 vitamin C Nutrition 0.000 abstract 1
- 239000011718 vitamin C Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 13
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 7
- 241000133262 Nauplius Species 0.000 description 7
- 229910052708 sodium Inorganic materials 0.000 description 7
- 239000011734 sodium Substances 0.000 description 7
- 235000013305 food Nutrition 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 241000595940 Notostraca Species 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 230000000284 resting effect Effects 0.000 description 4
- 229960002163 hydrogen peroxide Drugs 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention relates to the field of production of aquaculture live fodder, and provides a hatching culture medium of artemia cysts hatched by fairy shrimps, and provides a preparation method and applications of the hatching culture medium of artemia cysts. The preparation method comprises the following processing steps: (1) dissolving 20-30 parts of NaCl, 15-20 parts of KCl, 30-40 parts of MgSO4, and 10-15 parts of SrCl2 in 1000 parts of H2O, uniformly stirring, to obtain a mixed liquor; (2) disinfecting; (3) adding 0.1-0.2 parts of glucose, 0.05-0.08 parts of glucose oxidase, stirring to dissolve; (4) adding vitamin C and tea polyphenol, uniformly stirring; (5) centrifuging and taking clear liquid; (6) adding H2O2 in the clear liquid, to obtain a culture medium, and rapidly transferring the culture medium in a closed transparent container.
Description
Technical field
The present invention relates to the production field of aquaculture life food, particularly relate to a kind of incubation culture medium of brine shrimp eggs and preparation method and application.
Background technology
Fairy shrimp nauplius is generally used as the life food of aquaculture, especially as the life food that ocean fish, brine shrimp stage are early stage.Market does not generally have the life food that fairy shrimp nauplius is such, only have brine shrimp eggs, nauplius is obtained by brine shrimp eggs hatching.The hatching of brine shrimp eggs can complete in incubation culture medium.Brine shrimp eggs even reaches several years for a long time through fully dewatering and can surviving under the dry environment of the unglazed photograph of low temperature oxygen-free.The storage capacity of overlength and shorter time (in 24 hours) can produce the characteristic of free-swimming nauplius, become the source of life food the most easily of aquaculture.Brine shrimp eggs from natural world as collected salt pan, seashore and inland brine lake.In the brine shrimp eggs that these are collected, there is resting egg and the Diapausing egg of different proportion.By purified for brine shrimp eggs to remove disintegrating slag, wash with desalination, finally to carry out drying process and cold house's preservation, it is made to be in resting state.The quality of brine shrimp eggs is judged by evaluate parameter, wherein brine shrimp eggs can hatchability by the concern of people, because it directly indicates under artificial incubation condition, the quantity of the life food (nauplius) that can obtain from the brine shrimp eggs of some.Can hatchability usually representing with hatching percentage (H%) of brine shrimp eggs, namely refer to the number of the free-swimming nauplius hatched from 100 complete brine shrimp eggs.The brine shrimp eggs be under diapause or resting state can not continue to hatch into free-swimming nauplius, unless under they are in the condition promoting hatching.Therefore the hatching of brine shrimp eggs needs to complete in incubation culture medium, and incubation culture medium is generally treated clean sea water.In hatching process, the abiotic parameter controlling incubation culture medium comprises: salinity, pH value, oxygen concentration and water temperature.The parameter area that current each production unit adopts usually is: salinity 5 ~ 35g/ liter; PH > 8; Oxygen concentration > 5g/L; Water temperature 25 ~ 28 DEG C.In fact the hatching of brine shrimp eggs is an extremely complicated process, generally includes three phases: front phase grows, umbrella stage and actual incubation period.But the factor affecting brine shrimp eggs hatching process is a lot, the hatching process carried out according to above-mentioned given incubation condition often can not reach the hatching effect of expection, therefore need to take special diapauze deactivating procedures, such as, extend the freezing resting period of dry brine shrimp eggs, repeat water suction-dewatering cycle, in special chemical material is as hydrogenperoxide steam generator the short time hatching etc.Mostly use hydrogenperoxide steam generator to increase the hatching percentage of slag oxygenation at present, need to carry out a large amount of tests at different conditions and could obtain optimum hydrogenperoxide steam generator concentration and the parameter in processing time.But the optimum parameter obtained by said method is not of universal significance, because the brine shrimp eggs for different fairy shrimp strain or same strain different batches, optimum parameter is change.
Summary of the invention
Therefore, for above content, the invention provides a kind of incubation culture medium of brine shrimp eggs of fairy shrimp hatching being applicable to different strain, same strain different batches, a kind of preparation method and application of incubation culture medium of described brine shrimp eggs are provided simultaneously.
For achieving the above object, the present invention is achieved by the following technical solutions: a kind of incubation culture medium of brine shrimp eggs, comprises each raw material of following weight portion:
Wherein, described NaCl, KCl, MgSO
4, SrCl
2for chemical pure solid, described H
2o
2mass concentration be 30%.
Further improvement is: each raw material comprising following weight portion:
Further improvement is, comprises each raw material of following weight portion:
Further improvement is, described pH adjusting agent is citric acid, tartaric acid, sodium citrate, malic acid.
A preparation method for the incubation culture medium of brine shrimp eggs, comprises following treatment step:
(1) by the NaCl of 20 ~ 30 parts, 15 ~ 20 parts of KCl, 30 ~ 40 parts of MgSO
4, 10 ~ 15 parts of SrCl
2be dissolved in the H of 1000 parts
2in O, stir, obtain mixed liquor;
(2) carry out disinfection the mixed liquor that step (1) is obtained 20 ~ 30min under ultraviolet light;
(3) in the mixed liquor through sterilization, 0.1 ~ 0.2 part of glucose, 0.05 ~ 0.08 part of glucose oxidase is added successively, stirring and dissolving;
(4) add 2 ~ 3 parts of vitamine C sodiums, 0.5 ~ 0.8 part of Tea Polyphenols again, stir at 45 ~ 60 DEG C;
(5) material step (4) obtained is the centrifugal 10 ~ 15min of 300 ~ 2000r/min at rotating speed, removes sediment, gets clear liquid;
(6) H of 22 ~ 27 parts is added in obtained in step (5) clear liquid
2o
2, obtain medium, then medium transferred to rapidly in airtight transparent vessel.
An application for the incubation culture medium of brine shrimp eggs, be inoculated into by brine shrimp eggs in the obtained medium of claim 5, add manganese dioxide in the medium, illumination controls at 2000 ~ 2500lux.
Further improvement is: the consumption of described manganese dioxide is 2 ~ 12 weight portions.
Further improvement is: the inoculum concentration of described brine shrimp eggs is 0.01 ~ 0.05 times that cultivates basic weight.
By adopting preceding solution, the invention has the beneficial effects as follows: the incubation culture medium of brine shrimp eggs of the present invention, comprising each raw material of following weight portion: NaCl20 ~ 30 part, KCl15 ~ 20 part, MgSO
430 ~ 40 parts, SrCl
210 ~ 15 parts, vitamine C sodium 2 ~ 3 parts, glucose 0.1 ~ 0.2 part, glucose oxidase 0.05 ~ 0.08 part, Tea Polyphenols 0.5 ~ 0.8 part, pH adjusting agent 0.1 ~ 10 part, H
2o
222 ~ 27 parts, H
2o1000 part; Wherein, described NaCl, KCl, MgSO
4, SrCl
2for chemical pure solid, described H
2o
2mass concentration be 30%, containing various nutriments required in good year egg hatching process in raw material, add glucose oxidase in the medium, the diapauze mechanism of brine shrimp eggs can be destroyed, impel Diapausing egg during incubation to produce free-swimming artemia nauplii, can hatching efficiency be significantly improved, add vitamine C sodium in the medium and can prevent Tea Polyphenols from being slowed down the oxidized speed of Tea Polyphenols, pH adjusting agent can be used for the acid-base value regulating medium entirety, H
2o
2finally adding at medium preparing, and proceed to rapidly in airtight transparent vessel after the addition, prevent H
2o
2under the environment opened wide, slowly decomposite oxygen, in the application process of described medium, add manganese dioxide, make itself and H
2o
2reaction produces oxygen, and makes to have certain dissolved oxygen in medium, promotes the hatching of fairy shrimp.
Embodiment
Describe embodiments of the present invention in detail below with reference to specific embodiment, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
If do not specialize, the conventional means that the technological means adopted in embodiment is well known to those skilled in the art, the reagent adopted and product be also can business obtain.Source, the trade name of agents useful for same and be necessary to list its constituent person, all indicate when occurring first.
Embodiment one
An incubation culture medium for brine shrimp eggs, comprises each raw material of following weight portion:
Its preparation method, comprises following treatment step:
(1) by NaCl, 15gKCl, 30gMgSO of 20g
4, 10gSrCl
2be dissolved in the H of 1000g
2in O, stir, obtain mixed liquor;
(2) carry out disinfection the mixed liquor that step (1) is obtained 20min under ultraviolet light;
(3) in the mixed liquor through sterilization, 0.1g glucose, 0.05g glucose oxidase is added successively, stirring and dissolving;
(4) add 2g vitamine C sodium, 0.5g Tea Polyphenols again, stir at 45 DEG C;
(5) material step (4) obtained is the centrifugal 15min of 300r/min at rotating speed, removes sediment, gets clear liquid;
(6) H that 22g mass concentration is 30% is added in obtained in step (5) clear liquid
2o
2, obtain medium, then medium transferred to rapidly in airtight transparent vessel.
Its application method is, is inoculated in above-mentioned medium by brine shrimp eggs, adds manganese dioxide in the medium, and illumination controls at 2000lux, and the inoculum concentration of described brine shrimp eggs is 0.01 times that cultivates basic weight.The addition of described manganese dioxide is 10g.
Embodiment two
An incubation culture medium for brine shrimp eggs, comprises each raw material of following weight portion:
Wherein, described NaCl, KCl, MgSO
4, SrCl
2for chemical pure solid.
Its preparation method, comprises following treatment step:
(1) by NaCl, 17gKCl, 35gMgSO of 23g
4, 12gSrCl
2be dissolved in the H of 1000g
2in O, stir, obtain mixed liquor;
(2) carry out disinfection the mixed liquor that step (1) is obtained 25min under ultraviolet light;
(3) in the mixed liquor through sterilization, 0.1g glucose, 0.07g glucose oxidase is added successively, stirring and dissolving;
(4) add 2g vitamine C sodium, 0.6g Tea Polyphenols again, stir at 50 DEG C;
(5) material step (4) obtained is the centrifugal 12min of 1000r/min at rotating speed, removes sediment, gets clear liquid;
(6) H that 25g mass concentration is 30% is added in obtained in step (5) clear liquid
2o
2, obtain medium, then medium transferred to rapidly in airtight transparent vessel.
Its application, be inoculated in above-mentioned medium by brine shrimp eggs, add manganese dioxide in the medium, illumination controls at 2200lux; The inoculum concentration of described brine shrimp eggs is 0.02 times that cultivates basic weight.The consumption of described manganese dioxide is 5g.
Embodiment three
An incubation culture medium for brine shrimp eggs, comprises each raw material of following weight portion:
Wherein, described NaCl, KCl, MgSO
4, SrCl
2for chemical pure solid
Its preparation method, comprises following treatment step:
(1) by NaCl, 18gKCl, 38gMgSO of 25g
4, 14gSrCl
2be dissolved in the H of 1000g
2in O, stir, obtain mixed liquor;
(2) carry out disinfection the mixed liquor that step (1) is obtained 26min under ultraviolet light;
(3) in the mixed liquor through sterilization, 0.2g glucose, 0.08g glucose oxidase is added successively, stirring and dissolving;
(4) add 2.5g vitamine C sodium, 0.7g Tea Polyphenols again, stir at 50 DEG C;
(5) material step (4) obtained is the centrifugal 13min of 1500r/min at rotating speed, removes sediment, gets clear liquid;
(6) H that 26g mass concentration is 30% is added in obtained in step (5) clear liquid
2o
2, obtain medium, then medium transferred to rapidly in airtight transparent vessel.
Its application, be inoculated in above-mentioned medium by brine shrimp eggs, add manganese dioxide in the medium, illumination controls at 2500lux; The inoculum concentration of described brine shrimp eggs is 0.04 times that cultivates basic weight.The consumption of described manganese dioxide is 2g.
Embodiment four
An incubation culture medium for brine shrimp eggs, comprises each raw material of following weight portion:
Wherein, described NaCl, KCl, MgSO
4, SrCl
2for chemical pure solid.
Its preparation method, comprises following treatment step:
(1) by NaCl, 20gKCl, 40gMgSO of 30g
4, 15gSrCl
2be dissolved in the H of 1000g
2in O, stir, obtain mixed liquor;
(2) carry out disinfection the mixed liquor that step (1) is obtained 30min under ultraviolet light;
(3) in the mixed liquor through sterilization, 0.2g glucose, 0.08g glucose oxidase is added successively, stirring and dissolving;
(4) add 3g vitamine C sodium, 0.8g Tea Polyphenols again, stir at 60 DEG C;
(5) material step (4) obtained is the centrifugal 15min of 2000r/min at rotating speed, removes sediment, gets clear liquid;
(6) H that 27g mass concentration is 30% is added in obtained in step (5) clear liquid
2o
2, obtain medium, then medium transferred to rapidly in airtight transparent vessel.
Its application method is, is inoculated in above-mentioned medium by brine shrimp eggs, adds manganese dioxide in the medium, and illumination controls at 2500lux; The inoculum concentration of described brine shrimp eggs is 0.05 times that cultivates basic weight.The consumption of described manganese dioxide is 4g.
Wherein, in the present invention, manganese dioxide be mainly used in catalysis H
2o
2the more catalysis of its consumption is faster, add according to the actual needs, the amount needed for the higher manganese dioxide of total amount of medium matrix just adds accordingly at double, and described each raw material all can realize object of the present invention within the scope of following weight portion: NaCl20 ~ 30 part, KCl15 ~ 20 part, MgSO
430 ~ 40 parts, SrCl
210 ~ 15 parts, vitamine C sodium 2 ~ 3 parts, glucose 0.1 ~ 0.2 part, glucose oxidase 0.05 ~ 0.08 part, Tea Polyphenols 0.5 ~ 0.8 part, pH adjusting agent 0.1 ~ 10 part, H
2o
222 ~ 27 parts, H
2o1000 part.
Above, be only the embodiment utilizing this origination techniques content, the modification that any those skilled in the art use this creation to do, change, all belong to the scope of the claims that this creation is advocated, and be not limited to those disclosed embodiments.
Claims (8)
1. an incubation culture medium for brine shrimp eggs, comprises each raw material of following weight portion:
Wherein, described NaCl, KCl, MgSO
4, SrCl
2for chemical pure solid, described H
2o
2mass concentration be 30%.
2. the incubation culture medium of brine shrimp eggs according to claim 1, is characterized in that: each raw material comprising following weight portion:
3. the incubation culture medium of brine shrimp eggs according to claim 1, is characterized in that: each raw material comprising following weight portion:
4. the incubation culture medium of the brine shrimp eggs according to claim 1 or 2 or 3, is characterized in that, described pH adjusting agent is citric acid, tartaric acid, sodium citrate, malic acid.
5. a preparation method for the incubation culture medium of brine shrimp eggs, is characterized in that: comprise following treatment step,
(1) by the NaCl of 20 ~ 30 parts, 15 ~ 20 parts of KCl, 30 ~ 40 parts of MgSO
4, 10 ~ 15 parts of SrCl
2be dissolved in the H of 1000 parts
2in O, stir, obtain mixed liquor;
(2) carry out disinfection the mixed liquor that step (1) is obtained 20 ~ 30min under ultraviolet light;
(3) in the mixed liquor through sterilization, 0.1 ~ 0.2 part of glucose, 0.05 ~ 0.08 part of glucose oxidase is added successively, stirring and dissolving;
(4) add 2 ~ 3 parts of vitamine C sodiums, 0.5 ~ 0.8 part of Tea Polyphenols again, stir at 45 ~ 60 DEG C;
(5) material step (4) obtained is the centrifugal 10 ~ 15min of 300 ~ 2000r/min at rotating speed, removes sediment, gets clear liquid;
(6) H of 22 ~ 27 parts is added in obtained in step (5) clear liquid
2o
2, obtain medium, then medium transferred to rapidly in airtight transparent vessel.
6. an application for the incubation culture medium of brine shrimp eggs, is characterized in that: be inoculated into by brine shrimp eggs in the obtained medium of claim 5, add manganese dioxide in the medium, illumination controls at 2000 ~ 2500lux.
7. the application of the incubation culture medium of brine shrimp eggs according to claim 6, is characterized in that: the consumption of described manganese dioxide is 2 ~ 12 weight portions.
8. the application of the incubation culture medium of the brine shrimp eggs according to claim 6 or 7, is characterized in that: the inoculum concentration of described brine shrimp eggs is 0.01 ~ 0.05 times that cultivates basic weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510894932.XA CN105532579A (en) | 2015-12-08 | 2015-12-08 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510894932.XA CN105532579A (en) | 2015-12-08 | 2015-12-08 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105532579A true CN105532579A (en) | 2016-05-04 |
Family
ID=55812585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510894932.XA Pending CN105532579A (en) | 2015-12-08 | 2015-12-08 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105532579A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020190222A1 (en) * | 2019-03-19 | 2020-09-24 | Artkom Yem Sanayi̇ İç Ve Diş Ti̇caret Li̇mi̇ted Şi̇rketi̇ | A method which enables hatching of artemia eggs |
| CN111789068A (en) * | 2020-07-16 | 2020-10-20 | 滨州市胜英水产有限公司 | Fairy shrimp egg hatching equipment and hatching method thereof |
| CN111801021A (en) * | 2018-01-15 | 2020-10-20 | 泰国商波尔阿夸股份有限公司 | Method for producing live aquatic feed |
| CN113080108A (en) * | 2021-03-19 | 2021-07-09 | 温州大学 | Method for constructing diabetic zebra fish model |
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
| CN114680083A (en) * | 2022-05-05 | 2022-07-01 | 重庆医科大学 | Preparation method and application of sterile fairy shrimp |
| CN115399265A (en) * | 2022-09-01 | 2022-11-29 | 海南慈德高科技渔业有限公司 | Method for hatching artemia cysts |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU935044A1 (en) * | 1980-11-26 | 1982-06-15 | Всесоюзное Научно-Производственное Объединение По Рыбоводству | Method of producing naupliuses from crustaceous eggs |
| CN1471357A (en) * | 2000-10-05 | 2004-01-28 | INVE�����ɷ�����˾ | Method for producing free swimming artemia nauplii and packaged cysts for use in that method |
| CN102027891A (en) * | 2009-09-29 | 2011-04-27 | 天津市佳音生物饲料有限公司 | Method for increasing hatchability of artemia cysts |
| CN102342262A (en) * | 2010-08-02 | 2012-02-08 | 佛山市顺德区汉楚电子塑料制品有限公司 | Artemia ecologic toy and production method thereof |
| CN102524196A (en) * | 2012-03-23 | 2012-07-04 | 天津海友佳音生物科技股份有限公司 | Method for improving hatching rate of artemia cysts |
| CN103563860A (en) * | 2013-11-22 | 2014-02-12 | 天津海友佳音生物科技股份有限公司 | Method for increasing hatching rate of diapause artemia eggs in weak light environment |
| CN104273099A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for increasing eclosion rate of diapause artermia eggs at high temperature |
| CN104273098A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for hatching under-diapause brine shrimp eggs in ultraviolet light |
-
2015
- 2015-12-08 CN CN201510894932.XA patent/CN105532579A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU935044A1 (en) * | 1980-11-26 | 1982-06-15 | Всесоюзное Научно-Производственное Объединение По Рыбоводству | Method of producing naupliuses from crustaceous eggs |
| CN1471357A (en) * | 2000-10-05 | 2004-01-28 | INVE�����ɷ�����˾ | Method for producing free swimming artemia nauplii and packaged cysts for use in that method |
| CN102027891A (en) * | 2009-09-29 | 2011-04-27 | 天津市佳音生物饲料有限公司 | Method for increasing hatchability of artemia cysts |
| CN102342262A (en) * | 2010-08-02 | 2012-02-08 | 佛山市顺德区汉楚电子塑料制品有限公司 | Artemia ecologic toy and production method thereof |
| CN102524196A (en) * | 2012-03-23 | 2012-07-04 | 天津海友佳音生物科技股份有限公司 | Method for improving hatching rate of artemia cysts |
| CN103563860A (en) * | 2013-11-22 | 2014-02-12 | 天津海友佳音生物科技股份有限公司 | Method for increasing hatching rate of diapause artemia eggs in weak light environment |
| CN104273099A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for increasing eclosion rate of diapause artermia eggs at high temperature |
| CN104273098A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for hatching under-diapause brine shrimp eggs in ultraviolet light |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111801021A (en) * | 2018-01-15 | 2020-10-20 | 泰国商波尔阿夸股份有限公司 | Method for producing live aquatic feed |
| WO2020190222A1 (en) * | 2019-03-19 | 2020-09-24 | Artkom Yem Sanayi̇ İç Ve Diş Ti̇caret Li̇mi̇ted Şi̇rketi̇ | A method which enables hatching of artemia eggs |
| CN111789068A (en) * | 2020-07-16 | 2020-10-20 | 滨州市胜英水产有限公司 | Fairy shrimp egg hatching equipment and hatching method thereof |
| CN113080108A (en) * | 2021-03-19 | 2021-07-09 | 温州大学 | Method for constructing diabetic zebra fish model |
| CN113080108B (en) * | 2021-03-19 | 2022-08-02 | 温州大学 | Method for constructing diabetic zebra fish model |
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
| CN114680083A (en) * | 2022-05-05 | 2022-07-01 | 重庆医科大学 | Preparation method and application of sterile fairy shrimp |
| CN115399265A (en) * | 2022-09-01 | 2022-11-29 | 海南慈德高科技渔业有限公司 | Method for hatching artemia cysts |
| CN115399265B (en) * | 2022-09-01 | 2024-01-26 | 海南慈德高科技渔业有限公司 | Hatching method for artemia cysts |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105532579A (en) | Hatching culture medium of artemia cyst, and preparation method and application thereof | |
| CN104115775B (en) | A kind of migration type Atlantic salmon industrial artificial seedling rearing method | |
| CN102524196B (en) | Method for improving hatching rate of artemia cysts | |
| CN102027891B (en) | Method for increasing hatchability of artemia cysts | |
| CN104430095B (en) | Fresh water farming method of penaeus vannamei boone | |
| WO2008022569A1 (en) | Method to hatch free swimming nauplii from artemia cysts | |
| CN102630639A (en) | Shelling method of artemia cyst | |
| CN103563860B (en) | Method for increasing hatching rate of diapause artemia eggs in weak light environment | |
| CN103966100B (en) | The substratum of slaughterhouse's waste water cultivation Isochrysis galbana and different glue algae and cultural method | |
| CN104273100A (en) | Brine shrimp egg shelling method for decreasing hatching rate | |
| CN106577408B (en) | It is a kind of for improving the preparation method of the shelling slag oxygenation of hatching rate | |
| CN106688970A (en) | Method for artificially breeding fingerlings of siganus vulpinus in indoor environments | |
| EA017048B1 (en) | Method for producing free-swimming artemia nauplii and a set for aquaculture | |
| CN106508824A (en) | Pretreatment method for efficiently improving ova hatching rate in good year | |
| CN105746446B (en) | A kind of decladding method of artemia eggs | |
| CN102440211A (en) | Killifish culture method | |
| CN105900934B (en) | Application of the papain in terms of artemia eggs decladding | |
| US20040121056A1 (en) | Process for decapsulating crustacean or rotifer eggs | |
| CN110089466B (en) | Efficient shell removing treatment method for brine shrimp eggs | |
| CN107535434A (en) | A kind of nuisanceless brine shrimp eggs hatching method | |
| CN104285911A (en) | Method for hatching diapause brine shrimp eggs under blue light | |
| CN104273099A (en) | Method for increasing eclosion rate of diapause artermia eggs at high temperature | |
| Nurussalam et al. | The frequency of calcium and magnesium differences in recirculation systems for increasing production of mudcrab Scylla serrata seed | |
| CN104273098B (en) | A kind of method hatching diapause artemia cysts under ultraviolet light | |
| CN104285909A (en) | Red light environment based incubation method of diapause brine shrimp eggs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160504 |