CN103563860B - Method for increasing hatching rate of diapause artemia eggs in weak light environment - Google Patents
Method for increasing hatching rate of diapause artemia eggs in weak light environment Download PDFInfo
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- CN103563860B CN103563860B CN201310596343.4A CN201310596343A CN103563860B CN 103563860 B CN103563860 B CN 103563860B CN 201310596343 A CN201310596343 A CN 201310596343A CN 103563860 B CN103563860 B CN 103563860B
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- hatching
- tea polyphenols
- sodium
- diapause
- slag oxygenation
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- 230000012447 hatching Effects 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000005058 diapause Effects 0.000 title claims abstract description 17
- 241001247197 Cephalocarida Species 0.000 title claims abstract 7
- 235000013601 eggs Nutrition 0.000 title abstract 5
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 48
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 48
- 239000001963 growth medium Substances 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000002893 slag Substances 0.000 claims description 60
- 238000006213 oxygenation reaction Methods 0.000 claims description 59
- 238000011534 incubation Methods 0.000 claims description 52
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 29
- 229910052708 sodium Inorganic materials 0.000 claims description 29
- 239000011734 sodium Substances 0.000 claims description 29
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 claims description 28
- 239000011782 vitamin Substances 0.000 claims description 28
- 208000031513 cyst Diseases 0.000 claims description 16
- 238000010348 incorporation Methods 0.000 claims description 2
- 241001122767 Theaceae Species 0.000 abstract 2
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 abstract 2
- 241000238582 Artemia Species 0.000 description 24
- 239000002609 medium Substances 0.000 description 22
- 238000005286 illumination Methods 0.000 description 20
- 239000007788 liquid Substances 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 150000003839 salts Chemical class 0.000 description 11
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 210000004681 ovum Anatomy 0.000 description 6
- 241000133262 Nauplius Species 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 229910052736 halogen Inorganic materials 0.000 description 5
- 150000002367 halogens Chemical class 0.000 description 5
- 239000013535 sea water Substances 0.000 description 5
- 229920005479 Lucite® Polymers 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000004926 polymethyl methacrylate Substances 0.000 description 4
- 230000000284 resting effect Effects 0.000 description 4
- 239000003643 water by type Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000238426 Anostraca Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 108010046301 glucose peroxidase Proteins 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- Farming Of Fish And Shellfish (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Physical Water Treatments (AREA)
Abstract
The invention discloses a method for increasing the hatching rate of diapause artemia eggs in a weak light environment. The diapause artemia eggs are hatched in a hatching culture medium, and the artemia eggs are contacted with a mixture of tea polyphenol and vitamin C sodium; and the concentration ratio of the vitamin C sodium and the tea polyphenol which are introduced into the hatching culture medium is (1:4)-(1:5). The invention aims to provide the method for increasing the hatching rate of the diapause artemia eggs in the weak light environment; and with the adoption of the method, the hatching rate is increased, the stability of the hatching rate is guaranteed, and the stable hatching rate of a large hatching place is guaranteed.
Description
Technical field
The present invention relates to a kind of method improving diapause hatchability of artemia cysts, particularly a kind of method utilizing polyphenol, VC sodium effectively to improve diapause hatchability of artemia cysts under low light environment.
Background technology
Artemia nauplii is generally used as the life food of aquaculture, especially as the life food that ocean fish, brine shrimp stage are early stage.Market does not generally have the life food that artemia nauplii is such, only have slag oxygenation, nauplius hatches from slag oxygenation.The hatching of slag oxygenation can by completing in incubation culture medium.Slag oxygenation is through abundant dehydration and under the dry environment of the unglazed photograph of low temperature oxygen-free, its activity can be preserved and even be reached several years for a long time.The storage capacity of overlength and shorter time, as produced the characteristic of free-swimming nauplius in 24 hours, become the source of life food the most easily of aquaculture.
Slag oxygenation comes from halogen worm, and China is also referred to as fairy shrimp, and it raises up seed and can be divided into two kinds of modes, and one is oviparity, and one is ovoviviparity.Dislike slightly when natural environment becomes, such as lower the temperature, short of rain when, female halogen worm can produce resting egg, and these ovum can not hatch artemia nauplii in the short time after discharging, major part in them through a resting stage, then can just start hatching and growth.
When natural environment is beneficial to Artemia grow very much time, the breeding of halogen worm raises up seed in mode ovoviviparous, and namely free-swimming artemia nauplii directly spins off from female halogen polypide.
Slag oxygenation from natural world as collected salt pan, seashore and inland brine lake.In the slag oxygenation that these are collected, there is resting egg and the Diapausing egg of different proportion.By purified for slag oxygenation to remove disintegrating slag, wash with desalination, finally to carry out drying process and cold house's preservation, it is made to be in diapause status.
It is different for collecting from the specific bodies of water its characteristic of slag oxygenation come, and we are referred to as to come from different strains.Generally come from the Bohai Sea Gulf of China, the Great Salt lake of the U.S., the Shuanghu area in Tibet, the Ga Hai in Qinghai Province, the Alashan League in the Inner Mongol some salt lakes gather the Artemia cysts that comes through correct freezing processing and drying, more artemia larvae can both be obtained in the medium.And come from the Ebinur Lake of China, Russia, Kazakhstan a lot of salt lakes its performance of slag oxygenation always not like this, their hatching situation is often affected by the external environment very large, and hatch results is not very stable.
The quality of slag oxygenation is judged by evaluate parameter, wherein slag oxygenation hatchability can receive the concern of people most, because it directly indicates under artificial incubation condition, the quantity of the life food (nauplius) that can obtain from the slag oxygenation of some.Can hatchability usually representing with incubation rate (H%) of artemia sporocyst, namely refer to the number of the free-swimming nauplius hatched from 100 complete slag oxygenation.
Be in the slag oxygenation under diapause or resting state, can not continue to hatch into free-swimming nauplius, unless under being in the condition promoting hatching, therefore the hatching of diapause slag oxygenation needs to complete in incubation culture medium, incubation culture medium is generally treated clean sea water, and its abiotic parameter comprises: salinity, pH value, oxygen concentration, water temperature and illumination.The parameter area that current each production unit adopts usually, and the external packing that this scope generally all can be printed on slag oxygenation product illustrates upper: as salinity be 15 ~ 35g dissolving salt/liter; Hatching density is 1.5-3g/L, pH > 8; Oxygen concentration > 5mg/L; Water temperature 25 ~ 30 DEG C, the intensity of illumination on the water surface should be not less than 2000lux.
In order to the incubation rate of diapause slag oxygenation effectively can be improved, existing and the technology brought into use probably has 5 kinds in current industry.
The first uses hydrogen peroxide treatment slag oxygenation, or be directly added in medium.Russian Patent SU-A-935044 " obtaining the method for naupiar larva from the worm's ovum of Crustaceans ", have recorded and use hydrogen peroxide treatment slag oxygenation, the amount of adding when improving method and the process of hatchability of artemia cysts." cultivation of marine organisms bait " 106 pages have recorded the scope of adding hydrogen peroxide in hatching slag oxygenation process.The density that 116 pages, document " seawater living bait culture technique " have recorded artemia ovum hatching be generally no more than 5g dry weight/liter.
The second is the patent of Belgian INVE company application, application number 01817979.7 " producing the method for free-swimming artemia nauplii and the packaged cyst for the method " have recorded and uses peroxide to add in medium, improve the method for hatchability of artemia cysts, and the amount of adding.
The third is the patent of Belgian INVE company application, and the patent No. 200680035576.5 " improving the method for halogen worm diapausing cysts hatching percentage " have recorded the method and the addition that use Tea Polyphenols to improve hatchability of artemia cysts.4th kind is, Tianjin Jiayin Biology Feedstuff Co., Ltd.'s patent, the patent No.: the Chinese patent application of 200910070698.3, have recorded the method and the amount that use ozone to improve hatchability of artemia cysts, and the time of process.5th Zhong Shi Tianjin Haiyou Jiayin Biology Co., Ltd., the patent No.: the Chinese patent application of 201210080233.8, have recorded and add amount and the method that glucose peroxidase is used for effectively improving hatchability of artemia cysts in incubation culture medium.
In artemia ovum hatching process, the slag oxygenation of incubation rate less stable, the worm's ovum that are produced from such as some salt lakes of Russia, Chinese Ebinur Lake, Kazakhstan, generally when not having hydrogen peroxide to participate in, illumination is very important, in the industry during known hatching slag oxygenation, the requirement of illumination is that the illumination on the water surface is not less than 2000lux.But in actual production process, fishes and shrimps seedling hatchery, due to the restriction of condition, in-plantly on each hatching pail can not provide light source, even if provide illumination, because light source distance hatching pail is comparatively far away, the intensity of illumination of its water surface is also often less than 1000lux.(fluorescent lamp of independent 40 watts, when distance is more than one meter of distance, measures its intensity of illumination and is often less than 1000lux).So be not that each hatching pail can both obtain good illumination, the slag oxygenation at this moment in medium is owing to can not get good illumination, and the incubation rate of slag oxygenation is often unstable.
In laboratory conditions, the general fluorescent lamp that uses is as light source, and culture device is all very little, and liquid medium identity distance from fluorescent lamp very close to, medium water surface intensity of illumination generally can reach more than 2000LUX.Illumination is very sufficient, effectively can improve the incubation rate of slag oxygenation.But in the actual use procedure in hatchery, incubation equipment is often all very large, due to material, is not often transparent.Incubation rate is often lower than the hatch results that laboratory measures.Therefore improve the method for diapause hatchability of artemia cysts under being badly in need of a kind of low light level, and require that incubation rate is stablized.
Summary of the invention
The problem to be solved in the present invention improves the method for diapause hatchability of artemia cysts under being to provide a kind of low light level, this method not only improves incubation rate, and ensures that incubation rate is stablized, and is also adapted at large hatching place and ensures stable incubation rate.
In order to solve the problem, technical scheme provided by the invention is:
Improve a method for diapause hatchability of artemia cysts under low light environment, the hatching of described slag oxygenation is carried out in incubation culture medium, makes slag oxygenation contact the mixture of Tea Polyphenols and vitamine C sodium.
Further, the mixture of described Tea Polyphenols and vitamine C sodium is introduced in incubation culture medium.
Further, the vitamine C sodium introduced in described incubation culture medium and the concentration ratio of Tea Polyphenols are 1:4-1:5.
Further, the hatching density of described slag oxygenation in incubation culture medium is at 1.5-3g/L, and the ratio of Tea Polyphenols and vitamine C sodium mixture and slag oxygenation is: 1:120-1:40.
Further, the incorporation way of described Tea Polyphenols and vitamine C sodium mixture directly adds incubation culture medium or be wrapped on slag oxygenation to join incubation culture medium.
Because Tea Polyphenols itself is coloured, after it is dissolved in medium, medium is also coloured, blocks the irradiation of light.Especially, after Tea Polyphenols is oxidized, it is darker that color can become.This concentration Tea Polyphenols solution, the intensity of illumination that 30 centimeters record below liquid level is less than 100lux.And liquid level is 80 centimetres in whole hatching pail.Slag oxygenation can not get good illumination in hatching process, and incubation rate reduces.Use while Tea Polyphenols and add vitamine C sodium, the oxidized speed of polyphenol is obviously slack-off, very slow that the speed namely darkened becomes.The incubation rate of such slag oxygenation improves, and to become very stable for the incubation rate of slag oxygenation simultaneously.
Advantage of the present invention is: the incubation rate that effectively can improve diapause slag oxygenation, and within 24 hours, incubation rate reaches 85%.
Embodiment:
Embodiment 1
Any material artemia ovum hatching is not added under usual terms
Use transparent incubator and 300ml test tube (in vitro 250ml seawater) to hatch, liquid level is 20cm.The hatching density of slag oxygenation is 2g/l
The incubation condition of medium:
Salinity 30g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C,
Intensity of illumination on medium liquid level is 2800lux.
| The place of production of slag oxygenation | Incubation rate (%) |
| Great Yi draws lake, Wal | 58 |
| Ebinur Lake | 65 |
Embodiment 2 directly adds Tea Polyphenols
Use transparent incubator and 300ml test tube (in vitro 250ml seawater) to hatch, liquid level is 20cm.The hatching density of slag oxygenation is 2g/l
The incubation condition of medium:
Salinity 30g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C,
Intensity of illumination on medium liquid level is 2800lux.
Brooding time: 24 hours
Tea Polyphenols directly adds medium
Great Yi is used to draw lake, Wal slag oxygenation
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Incubation rate (%) | 58 | 75 | 82 | 85 | 85 | 85 | 70 | 65 | 60 | 54 | 40 |
Use Xinjiang, China Ebinur Lake slag oxygenation
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Incubation rate (%) | 65 | 70 | 75 | 85 | 85 | 85 | 80 | 75 | 60 | 55 | 50 |
After hatching terminates, examine under a microscope, the concentration that more it is shocking when Tea Polyphenols reaches 100mg/l when rising, a lot of artemia larvae after broken shell just stopping hatched, can not move about, or simply only expose a chorion part.Analyze reason, may be reduce pH value after excess polyphenols joins medium, meanwhile, serious hinders the irradiation of light to slag oxygenation.Have impact on normally carrying out of hatching.
Embodiment 3 adds Tea Polyphenols, uses large culture device instead
Use above 500L open, below black non transparent lucite hatching pail is hatched. and the hatching density of slag oxygenation is 2g/l
Hatching cylinder maritime interior waters 300L, liquid level is about 80cm
The incubation condition of medium: seawater
Salinity 30g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C,
Intensity of illumination on medium liquid level is 2800lux.
Brooding time: 24 hours
Parcel: slag oxygenation is immersed in Tea Polyphenols solution, makes Tea Polyphenols be wrapped in slag oxygenation on the surface.
Great Yi is used to draw lake, Wal slag oxygenation
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Incubation rate (%) | 58 | 70 | 72 | 75 | 75 | 70 | 65 | 60 | 60 | 50 | 40 |
Use Xinjiang, China Ebinur Lake slag oxygenation
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Incubation rate (%) | 65 | 70 | 75 | 75 | 75 | 72 | 65 | 60 | 55 | 52 | 45 |
Found by the contrast of embodiment 2 and embodiment 3, if only add Tea Polyphenols, after expanding production, incubation rate obviously reduces.
Embodiment 4 directly adds Tea Polyphenols and vitamine C sodium
Use above 500L open, below black non transparent lucite hatching pail is hatched. and the hatching density of slag oxygenation is 1.5g/l
Hatching cylinder maritime interior waters 300L, liquid level is about 80cm
The incubation condition of medium:
Salinity 30g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C,
Intensity of illumination on medium liquid level is 2800lux.
Brooding time: 24 hours
Directly add Tea Polyphenols and vitamine C sodium
Great Yi is used to draw lake, Wal slag oxygenation (vitamine C sodium and Tea Polyphenols ratio are 1:4)
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Vitamine C sodium (mg/l) | 0 | 5 | 7.5 | 10 | 12.5 | 15 | 17.5 | 20 | 22.5 | 25 | 27.5 |
| Incubation rate (%) | 58 | 75 | 85 | 80 | 75 | 70 | 65 | 61 | 60 | 55 | 50 |
Use Xinjiang, China Ebinur Lake slag oxygenation (vitamine C sodium and Tea Polyphenols ratio are 1:4)
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Vitamine C sodium (mg/l) | 0 | 5 | 7.5 | 10 | 12.5 | 15 | 17.5 | 20 | 22.5 | 25 | 27.5 |
| Incubation rate (%) | 65 | 72 | 85 | 80 | 75 | 72 | 71 | 70 | 68 | 60 | 55 |
Embodiment 5 directly adds Tea Polyphenols and vitamine C sodium
Use above 500L open, below black non transparent lucite hatching pail is hatched. and the hatching density of slag oxygenation is 3g/l
Hatching cylinder maritime interior waters 300L, liquid level is about 80cm
The incubation condition of medium:
Salinity 30g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C,
Intensity of illumination on medium liquid level is 2800lux.
Brooding time: 24 hours
Direct interpolation
Great Yi is used to draw lake, Wal slag oxygenation (vitamine C sodium and Tea Polyphenols ratio are 1:5)
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Vitamine C sodium (mg/l) | 0 | 4 | 6 | 8 | 10 | 12 | 14 | 16 | 18 | 20 | 22 |
| Incubation rate (%) | 58 | 80 | 85 | 85 | 85 | 85 | 70 | 64 | 60 | 58 | 45 |
Use Xinjiang, China Ebinur Lake slag oxygenation (vitamine C sodium and Tea Polyphenols ratio are 1:5)
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Vitamine C sodium (mg/l) | 0 | 4 | 6 | 8 | 10 | 12 | 14 | 16 | 18 | 20 | 22 |
| Incubation rate (%) | 65 | 78 | 85 | 85 | 86 | 85 | 70 | 65 | 64 | 55 | 45 |
Embodiment 6 Tea Polyphenols and vitamine C sodium
Use above 500L open, below black non transparent lucite hatching pail is hatched. and the hatching density of slag oxygenation is 2g/l
Hatching cylinder maritime interior waters 300L, liquid level is about 80cm
The incubation condition of medium:
Salinity 30g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C,
Intensity of illumination on medium liquid level is 2800lux.
Brooding time: 24 hours
Parcel adds Tea Polyphenols and vitamine C sodium
Great Yi is used to draw lake, Wal slag oxygenation (vitamine C sodium and Tea Polyphenols ratio are 1:5)
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Vitamine C sodium (mg/l) | 0 | 4 | 6 | 8 | 10 | 12 | 14 | 16 | 18 | 20 | 22 |
| Incubation rate (%) | 60 | 85 | 86 | 86 | 80 | 76 | 71 | 66 | 61 | 60 | 50 |
Use Xinjiang, China Ebinur Lake slag oxygenation (vitamine C sodium and Tea Polyphenols ratio are 1:5)
| The amount (mg/l) of Tea Polyphenols | 0 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 |
| Vitamine C sodium (mg/l) | 0 | 4 | 6 | 8 | 10 | 12 | 14 | 16 | 18 | 20 | 22 |
| Incubation rate (%) | 66 | 85 | 86 | 85 | 78 | 76 | 71 | 66 | 65 | 56 | 50 |
Can be found out by embodiment 4-6, add Tea Polyphenols and vitamine C sodium, under magnification, the incubation rate of slag oxygenation also can maintain higher level simultaneously, and especially when the quantity of adding is suitable, incubation rate reaches more than 85%.
Claims (2)
1. one kind is improved the method for diapause hatchability of artemia cysts under low light environment, it is characterized in that, the mixture of Tea Polyphenols and vitamine C sodium is introduced in incubation culture medium, the vitamine C sodium introduced in described incubation culture medium and the concentration ratio of Tea Polyphenols are 1:4-1:5, the hatching density of described slag oxygenation in incubation culture medium is at 1.5-3g/L, and the mass ratio of vitamine C sodium and Tea Polyphenols mixture and slag oxygenation is: 1:120-1:40.
2. a kind of method improving diapause hatchability of artemia cysts under low light environment according to claim 1, it is characterized in that, the incorporation way of described Tea Polyphenols and vitamine C sodium mixture directly adds incubation culture medium or be wrapped on slag oxygenation to join incubation culture medium.
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| CN104285911A (en) * | 2014-10-21 | 2015-01-21 | 天津海友佳音生物科技股份有限公司 | Method for hatching diapause brine shrimp eggs under blue light |
| CN104273098B (en) * | 2014-10-21 | 2016-08-24 | 天津海友佳音生物科技股份有限公司 | A kind of method hatching diapause artemia cysts under ultraviolet light |
| CN104488828B (en) * | 2014-12-18 | 2017-06-16 | 天津海友佳音生物科技股份有限公司 | A kind of method for improving incubation rate after artemia sporocyst shells |
| CN105815276A (en) * | 2015-01-09 | 2016-08-03 | 天津丰年水产养殖有限公司 | Special culturing salt for increasing hatchability of brine shrimp eggs |
| CN105532579A (en) * | 2015-12-08 | 2016-05-04 | 厦门金益海生物科技有限公司 | Hatching culture medium of artemia cyst, and preparation method and application thereof |
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| CN100339001C (en) * | 2004-11-12 | 2007-09-26 | 付瑞岭 | Artemia ovum hatching method with high hatchability rate |
| EP1767101A1 (en) * | 2005-09-26 | 2007-03-28 | Inve Technologies N.V. | Method to enhance hatching percentage of Artemia diapauzing cysts |
| CN101081015A (en) * | 2006-08-18 | 2007-12-05 | 天津市佳音生物饲料有限公司 | Method for producing artemia nauplius from artemia sporangiocyst |
| CN101731181A (en) * | 2008-11-10 | 2010-06-16 | 谢坤 | Method for hatching artemia cysts |
| CN102027891B (en) * | 2009-09-29 | 2013-03-13 | 天津市佳音生物饲料有限公司 | Method for increasing hatchability of artemia cysts |
| CN102524196B (en) * | 2012-03-23 | 2013-09-04 | 天津海友佳音生物科技股份有限公司 | Method for improving hatching rate of artemia cysts |
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