CN105815276A - Special culturing salt for increasing hatchability of brine shrimp eggs - Google Patents
Special culturing salt for increasing hatchability of brine shrimp eggs Download PDFInfo
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- CN105815276A CN105815276A CN201510011664.2A CN201510011664A CN105815276A CN 105815276 A CN105815276 A CN 105815276A CN 201510011664 A CN201510011664 A CN 201510011664A CN 105815276 A CN105815276 A CN 105815276A
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Abstract
The invention discloses special culturing salt for increasing the hatchability of brine shrimp eggs. The incubation of the brine shrimp eggs is performed in an incubation culture medium, different substances are added to the incubation culture medium and are mixed to prepare special salt in the solid powder manner according to the following corresponding percentage by weight: 85-90% of sodium chloride, 3-5% of magnesium chloride, 2-4% of magnesium sulphate, 2-3% of calcium chloride, 2-3% of potassium chloride, 1-2% of sodium bicarbonate, 0.2-0.8% of sodium carbonate, and 0.1-0.15% of calcium peroxide. The concentration of the incubation culture medium is 28-35 g/L, and the density of the brine shrimp eggs in the incubation culture medium is less than or equal to 3 g of dried brine shrimp eggs per liter of the incubation culture medium. The method not only guarantees the salinity desired by the incubation of the brine shrimp eggs, but also does not cause a side effect on the incubation of the brine shrimp eggs; most important of all, varied component salt substances and an appropriate amount of the calcium peroxide can destroy the diapause eggs in the brine shrimp eggs, and the brine shrimp eggs are promoted to incubate brine shrimp nauplius which can freely go, so that the hatchability of the brine shrimp eggs reaches 80% or above.
Description
Technical field:
The present invention relates to a kind of cultivation special-purpose salt promoting artemia ovum hatching, especially relate to a kind of method improving hatchability of artemia cysts.
Background technology:
Artemia (BrineShrimp) is also referred to as saline fairy shrimp, is to live in a kind of biology in saline.Folks of china is also referred to as salt insect or shrimps.China coast salt pan is long-pending reaches 34.5 ten thousand hectares, and large and medium-sized salt lake, inland has nearly thousand, in the water that these are wide, contains the abundantest artemia resource.Artemia is because containing abundant nutrition, as a kind of important food organisms and good laboratory animal material, being constantly subjected to the extensive attention of people.When living environment occurs to deteriorate (as temperature reduces, salinity increases), in order to continue population, artemia can discharge ovum, is commonly called as " artemia cysts ".Because artemia cysts can realize lyophilizing, easily preserve, can be with incubating, the nauplius hatched is the best bait of aquatic biological, is that other natural bait is irreplaceable with the artificial feedstuff institute that develops, is extensively applied by culture fishery.
The ecological condition of artemia ovum hatching is the key factor affecting hatchability of artemia cysts.The artemia cysts that quality is the best, if incubation condition control is improper, incubation rate also can become the lowest.In China, the essential condition controlling incubation rate has salinity, pH value, oxygen concentration and water temperature etc., wherein the composition of salt and salinity not only affect growth and the breeding of artemia, and affect the hatching of Artemia cysts, and relevant salt composition is less to the influence research of artemia hatching.Hydrogenperoxide steam generator or addition oxidase is the most directly used to improve the incubation rate of artemia cysts, need just to be can determine that suitable concentration proportioning by great many of experiments, and require that the joining day is the strictest, subsequent treatment is cumbersome, the membership that adds of a large amount of hydrogen peroxide causes the too high death that may cause artemia nauplii of environment of hatching pH, also various artemia cysts is not had blanket meaning.
The present invention is directed to the defect of prior art, it is provided that a kind of suitable special-purpose salt formula promoting artemia ovum hatching, can realize commonly using.
Summary of the invention:
It is an object of the invention to overcome the defect of prior art, it is provided that a kind of improve hatchability of artemia cysts and method simple to operate.
A kind of cultivation special-purpose salt improving hatchability of artemia cysts, the hatching of described artemia cysts is carried out in incubation culture medium, it is characterized in that: addition different material in described incubation culture medium: sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium bicarbonate, sodium carbonate and calper calcium peroxide, described different material is mixed and made into special-purpose salt with solidapowder form, the dry artemia cysts of described artemia cysts density≤3g in described incubation culture medium/L incubation culture medium.
Described special-purpose salt separates individually packaging with solidapowder form and described artemia cysts, is added directly in incubation culture medium during use.The time adding described special-purpose salt in described incubation culture medium is that described artemia cysts fully absorbs water in 2 hours, in preferably 1 hour.
As preferably, different material described in described special-purpose salt and corresponding weight are respectively as follows: sodium chloride 85-90%, magnesium chloride 3-5%, magnesium sulfate 2-4%, calcium chloride 2-3%, potassium chloride 2-3%, sodium bicarbonate 1-2%, sodium carbonate 0.2-0.8%, calper calcium peroxide 0.1-0.15%.
As preferably, the concentration of described special-purpose salt is 28-35g special-purpose salt/L incubation culture medium.
As preferably, described artemia cysts density in artemia hatching solution is the dry artemia cysts of 1-2.5g/L incubation culture medium.
The method of the present invention both ensure that the salinity needed for artemia ovum hatching, the hatching of artemia cysts will not there be is side effect, the most important salts substances because of various ingredients and calper calcium peroxide can destroy the Diapausing egg in artemia cysts, promote that artemia ovum hatching goes out free-swimming artemia nauplii, thus improve the incubation rate of artemia cysts, can reach more than 80%.
Detailed description of the invention
Described artemia cysts is treated dry artemia cysts.Incubation culture medium is treated natural cleaning fresh water, to control be 28 ± 2 DEG C, fully absorb water under inflated condition in artemia hatching solution temperature to be dried artemia cysts, use and add sodium chloride, magnesium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium bicarbonate, sodium carbonate and the method for calper calcium peroxide, control suitable salinity, to reach to improve the purpose of hatchability of artemia cysts.By experiment it needs to be determined that the component ratio of different material in suitable artemia cysts density and special-purpose salt.
It should be noted that the artemia cysts for different batches, its salinity required is not quite similar, but we have found that incubation culture medium is in the range of certain salinity 28-35 ‰, hatching effect has reached optimum state, increase along with salinity, hatching is not had obvious beneficial effect, is free from side effects yet, so the salinity of incubation culture medium is controlled between 28-35 ‰.In the following embodiments, the component of special-purpose salt different material in allotment incubation culture medium, is 30 ‰ to consider according to controlling salinity.
1, suitable artemia cysts density is determined
Artemia cysts is pressed the different densities packet of the dry artemia cysts of 0.5-3g/L incubation culture medium, in identical incubation culture medium, add identical special-purpose salt, its addition is 30g/L incubation culture medium, the wherein component of the different material sodium chloride than respectively 85% in special-purpose salt, the magnesium chloride of 3%, the magnesium sulfate of 3%, the calcium chloride of 3%, the potassium chloride of 3%, the sodium bicarbonate of 2%, the sodium carbonate of 0.8%, the calper calcium peroxide of 0.15%, in identical brooding time (48 hours), obtains the incubation rate such as table 1 of different artemia cysts density:
The impact on incubation rate of the table 1 artemia cysts density
Can be seen that artemia cysts density all can reach optimal hatching effect at 0.5-3g/L from this, illustrate that artemia cysts density is little on incubation rate impact, this experiment is chosen 2.5g/L artemia cysts density and is carried out following experiment.
2, the component ratio of different material in special-purpose salt is determined
Sodium chloride, magnesium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium bicarbonate, sodium carbonate and calper calcium peroxide component are grouped such as table 2 than by 0-100% special-purpose salt, in identical incubation culture medium, add the special-purpose salt of 30g/L incubation culture medium, add the artemia cysts of 2.5g/L, in identical brooding time (48 hours), obtain different component than time incubation rate such as table 3.
Table 2 special-purpose salt different material proportion of composing (unit: %)
The impact on incubation rate of the table 3 different material composition
As can be seen from the above table, in special-purpose salt, the component of different material is more optimum than with A7 group, and incubation rate reaches the highest.Because the artemia cysts density in culture medium is 2.5g/L, and the concentration that special-purpose salt is in the medium is 30g/L, so the special-purpose salt needed for every gram of artemia cysts is 12g, corresponding required optimal special-purpose salt composition amounts is respectively the magnesium chloride of the sodium chloride of 10.21g, 0.36g, the magnesium sulfate of 0.36g, the calcium chloride of 0.36g, the potassium chloride of 0.36g, the sodium bicarbonate of 0.24g, the sodium carbonate of 0.096g, the calper calcium peroxide of 0.018g.The special-purpose salt mixed individually is packed with the most appropriate artemia cysts, finally carries out subpackage.Need in identical incubation culture medium, when using, be mixed in proportion directly use.The present invention is simple to operate, and using effect is good.
Described special-purpose salt separates individually packaging with solidapowder form and described artemia cysts, is added directly in incubation culture medium during use.The time adding described special-purpose salt in described incubation culture medium is that described artemia cysts fully absorbs water in 2 hours, in preferably 1 hour.
The invention have the advantages that
1. the influence factor of artemia ovum hatching is optimized by the present invention by different material composition first.
Calper calcium peroxide in artemia hatching solution the most of the present invention utilizes free radical ion that Diapausing egg can be promoted to produce free-swimming nauplius.
Sodium bicarbonate in artemia hatching solution the most of the present invention can directly control the suitable ph of artemia hatching solution.
Artemia hatching solution special-purpose salt formula the most of the present invention meets the salinity of artemia ovum hatching, pH and catalysis incubation condition simultaneously, decreases other chemistry or addition of biological substance, reduces and pollute, operate the simplest.
5. the present invention improves the efficiency of artemia ovum hatching, and cost reduces, and can realize the hatching of high-volume artemia cysts.
Embodiment disclosed herein is exemplary, and it is merely to explain the present invention, and is not limitation of the present invention, within the improvement that those skilled in the art will envision that and extension are included in protection scope of the present invention.
Claims (7)
1. the cultivation special-purpose salt improving hatchability of artemia cysts, the hatching of described artemia cysts is carried out in incubation culture medium, it is characterized in that: addition different material in described incubation culture medium: magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium bicarbonate, sodium carbonate and calper calcium peroxide, described different material is mixed and made into special-purpose salt with solidapowder form, the dry artemia cysts of described artemia cysts density≤3g in described incubation culture medium/L incubation culture medium.
Method the most according to claim 1, it is characterised in that: described special-purpose salt separates individually packaging with solidapowder form and described artemia cysts, is added directly in incubation culture medium during use.
Method the most according to claim 2, it is characterised in that: the time adding described special-purpose salt in described incubation culture medium is that described artemia cysts fully absorbed water in 2 hours.
Method the most according to claim 3, it is characterised in that: the time adding described special-purpose salt in described incubation culture medium is that described artemia cysts fully absorbed water in 1 hour.
5. according to the method according to any one of Claims 1 to 4, it is characterised in that: the density of described artemia cysts is 1～2.5g dry artemia cysts/L incubation culture medium.
6. according to the method according to any one of Claims 1 to 4, it is characterised in that: the concentration of described special-purpose salt is 28～35g special-purpose salts/L incubation culture medium.
7. according to the method according to any one of Claims 1 to 4, it is characterized in that: the corresponding weight of different material described in described special-purpose salt is respectively as follows: sodium chloride 85-90%, magnesium chloride 3-5%, magnesium sulfate 2-4%, calcium chloride 2-3%, potassium chloride 2-3%, sodium bicarbonate 1-2%, sodium carbonate 0.2-0.8%, calper calcium peroxide 0.1-0.15%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
| CN108432676A (en) * | 2018-03-12 | 2018-08-24 | 宁波牧海人海洋科技有限公司 | A kind of special cultivation salt formula of prawn |
| CN112602636A (en) * | 2020-12-18 | 2021-04-06 | 天津科技大学 | High-density artemia egg hatching method fully utilizing hatching water body |
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
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| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
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| CN108432676A (en) * | 2018-03-12 | 2018-08-24 | 宁波牧海人海洋科技有限公司 | A kind of special cultivation salt formula of prawn |
| CN112602636A (en) * | 2020-12-18 | 2021-04-06 | 天津科技大学 | High-density artemia egg hatching method fully utilizing hatching water body |
| CN112602636B (en) * | 2020-12-18 | 2022-01-18 | 天津科技大学 | High-density artemia egg hatching method fully utilizing hatching water body |
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
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