CN105746446B - A kind of decladding method of artemia eggs - Google Patents
A kind of decladding method of artemia eggs Download PDFInfo
- Publication number
- CN105746446B CN105746446B CN201610314079.4A CN201610314079A CN105746446B CN 105746446 B CN105746446 B CN 105746446B CN 201610314079 A CN201610314079 A CN 201610314079A CN 105746446 B CN105746446 B CN 105746446B
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- CN
- China
- Prior art keywords
- decladding
- ovum
- artemia eggs
- artemia
- shelling
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The present invention provides a kind of decladding methods of artemia eggs, include the following steps：（1）By commodity artemia eggs freshwater soaking, make the abundant water swelling of ovum.（2）Artemia eggs is isolated, is placed it in the shelling liquid containing trypsase, is slowly stirred.（3）When the color of observation ovum gradually becomes orange red by brown, terminator is added in.（4）It is stood after Aerated stirring, isolates shelled egg, can be used to hatch or directly feed after simply being rinsed with seawater.The invention belongs to bioanalysis decladding, raw materials used is natural products, to shelled egg without chemical contamination, is had no adverse effects to directly feeding or subsequently hatching, and effect stability.
Description
Technical field
The invention belongs to artemia eggs applied technical fields, and in particular to a kind of decladding method of artemia eggs.
Background technology
Artemia is called fairy shrimp, is universal small-sized shell-fish, moves in the high saltwater such as salt lake, saltern.
Artemia eggs is good aquatic fry growing bait, is played an important roll in culture fishery.Artemia chorion is iron content lipoprotein, fishes and shrimps
Seedling can not digest, therefore generally require and first hatch artemia larvae, then again using the young as bait feeding.Ovum after hatching
Shell and the young are not easy to be completely separated, and easily cause part and have hatched the young and outwell and be lost or chorion and the young are adopted with chorion
Food causes fishes and shrimps seedling intestinal obstruction dead.It can directly be fed after artemia eggs decladding, the young can also be hatched.Due to there is no ovum
Shell, it is possible to reduce energy expenditure during young broken shell is conducive to retain more nutrition.In fishes and shrimps nursery, the expense of artemia eggs
Often account for nursery 50%-70% costs.By the method for decladding, hatching rate can be improved and artemia larvae is made to retain more battalion
It supports, while the low-quality ovum that a large amount of hatching rates can also be utilized low, reduces seedling cost, it is significant to nursery.
Artemia eggs decladding at present mostly using chemical method, i.e., dissolves chorion, after decladding with sodium hypochlorite and sodium hydroxide solution
Reaction can not terminate immediately, need to pull shelled egg out cleaning and remove chlorine residue, during this chlorine residue of solution continue to the ovum that shells
Effect, damage are larger；Solution temperature easily increases in shell process simultaneously, influences hatching rate, needs to take cooling measure；Behaviour
It is very big on hatching rate influence to make process, technique is unstable；And the chlorine residue of ovum surface is difficult to thoroughly remove, and has chlorine distinctive smell when feeding
Aquatic seedling feeding is influenced, often also needs to addition phagostimulant.
Invention content
The present invention provides a kind of technical solutions of artemia eggs bioanalysis decladding, it is therefore intended that solves above-mentioned chemical method decladding
Existing deficiency.
1. by commodity artemia eggs freshwater soaking 2-4 hours, Aerated stirring makes the abundant water swelling of ovum；
2. isolating artemia eggs, place it in 10 times of weight shelling liquids, 60r/min is stirred at 30 DEG C of temperature；
3. 0.5-5 hours decladding time, the color for observing ovum gradually becomes orange red by brown, adds in terminator；
4. Aerated stirring 10 minutes, stand, shelled egg detached after being layered, with can be used to after seawater flushing hatching or
Directly feed.
Fresh water used need to dechlorinate if it is tap water, mitigate the damage to ovum in advance in the above process.
The shelling liquid ingredient is：Trypsase 0.1%-2%, EDTA0.02%, remaining is fresh water.
The terminator ingredient is：The soybean that 10 times of trypsase weight and raw soya-bean milk prepared by the water of 5 times of soybean weight.
In order to obtain better decladding effect, the decladding time is preferably 2-3 hours in step 3, and the artemia of different sources is gone
The shell time is different；Trypsin amount is preferably 0.5%-1.2% in shelling liquid.
Lipoprotein agent structure in shell is hydrolyzed and removed by trypsase, other a small amount of remaining insoluble components are violent
By the shear action of liquid from egg membrane sur-face peeling during Aerated stirring.Contain trypsin inhibitor in soybean, including K
Unitz classes inhibitor and BowmanBirk class inhibitor can inhibit rapidly the activity of trypsase in shelling liquid,
Egg membrane is protected not to be damaged, is used in the form of raw soya-bean milk, it is not only simple and practicable to reduce cost.
Compared with prior art, beneficial effects of the present invention：
The invention belongs to bioanalysis decladding, raw materials used is natural products, chemical raw material is not introduced, to shelled egg without change
Pollution is learned, has no adverse effects to subsequently hatching or directly feeding, reduces operation difficulty, and decladding effect stability.
Specific embodiment
The present invention is described in further detail below by embodiment, but the present invention is not limited to following embodiment party
Formula.
Embodiment 1：
1. by 500 grams of artemia eggs freshwater soaking 3 hours, Aerated stirring makes the abundant water swelling of ovum.If fresh water used
It for tap water, need to dechlorinate in advance, mitigate the damage to shelled egg.
2. isolating artemia eggs, place it in 5 liters of shelling liquids, 60r/min is stirred at 30 DEG C of temperature.Shelling liquid ingredient
For：Trypsase 0.8%, EDTA0.02%.
3. decladding time 2 h, when the color of ovum gradually becomes orange red by brown, terminator is added in.Terminator ingredient
For：Raw soya-bean milk prepared by 400 grams of soybean and 2 kg of water, soybean trypsin inhibitor therein can effectively inhibit trypsase
Activity prevents its destruction to egg membrane.
4. pneumatic head is placed in solution bottom, charge flow rate reaches solution in tumbling state, and Aerated stirring 10 minutes is quiet
It puts, after solution layering, shelled egg is separated, can be used to hatch or directly feed after simply being rinsed with seawater.Because of pancreas
Enzyme has been suppressed activity, and the calcium ions and magnesium ions in seawater also can inhibit its activity, then egg membrane can not be made after seawater dilutes
It is harmless to artemia larvae and fishes and shrimps seedling into damage, save time for being largely used to clean repeatedly and artificial.
Embodiment 2：Influence of the different decladding methods to artemia ovum hatching
Artemia eggs of the place of production for Shandong with batch is used, in natural light irradiation, salinity 30 ‰, 28 DEG C of temperature, brooding time
For 24 hours and under other conditions unanimous circumstances, two methods are taken in different disposal time corresponding shelling rate and corresponding shelled egg
Hatching rate, compare influence of two different decladding methods to artemia ovum hatching, the results are shown in Table 1.Wherein：Control group is
Normal incubation, not decladding；Chemical method is combined for sodium hypochlorite and sodium hydroxide；The hatching rate of two kinds of decladding methods is shelled egg
Hatching rate.
Influence of the 1 different disposal method of table to artemia ovum hatching
Using in chemical method shell process, hatching rate and the shelling rate of shelled egg are negatively correlated, and the decladding time the long, goes
Shell rate is higher, and hatching rate is lower.Although decladding egg hatching rate when shelling rate is low is higher than control group, there is considerable part at this time
Ovum there is no decladding, so ovum overall hatching rate improve it is smaller；When complete decladding, overall hatching rate compares normal incubation instead
It is low.Using the method for the present invention decladding, action temperature and, ovum is damaged small, the decladding egg hatching rate when shelling rate is high is still higher,
The overall hatching rate of ovum is made to improve a lot, and is directly fed or suitable for hatching.
Claims (1)
1. a kind of decladding method of artemia eggs, it is characterised in that：Include the following steps,
（1）By commodity artemia eggs freshwater soaking 2-4 hours, Aerated stirring makes the abundant water swelling of ovum；
（2）Artemia eggs is isolated, is placed it in 10 times of weight shelling liquids, 60r/min is stirred at 30 DEG C of temperature；
（3）0 .5-5 hours decladding time when observing the color of ovum and gradually becoming orange red by brown, added in terminator；
（4）Aerated stirring 10 minutes stands, shelled egg is isolated after being layered, with can be used to hatching or straight after seawater flushing
It connects and feeds;
Step（2）Middle shelling liquid ingredient is：Trypsase 0.1%-2%, EDTA 0.02%, remaining is fresh water；Step（3）Middle termination
Agent ingredient is：The soybean that 10 times of trypsase weight and raw soya-bean milk prepared by the water of 5 times of soybean weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610314079.4A CN105746446B (en) | 2016-05-13 | 2016-05-13 | A kind of decladding method of artemia eggs |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610314079.4A CN105746446B (en) | 2016-05-13 | 2016-05-13 | A kind of decladding method of artemia eggs |
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| Publication Number | Publication Date |
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| CN105746446A CN105746446A (en) | 2016-07-13 |
| CN105746446B true CN105746446B (en) | 2018-07-06 |
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| CN201610314079.4A Expired - Fee Related CN105746446B (en) | 2016-05-13 | 2016-05-13 | A kind of decladding method of artemia eggs |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106577408B (en) * | 2016-11-22 | 2019-09-03 | 江苏好润生物科技有限公司 | It is a kind of for improving the preparation method of the shelling slag oxygenation of hatching rate |
| CN106689063B (en) * | 2016-12-07 | 2020-03-17 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma canker of rice borer |
| CN110055213A (en) * | 2019-04-23 | 2019-07-26 | 中国海洋大学 | A kind of separation method of dwarf clam egg membrane |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101843237A (en) * | 2009-03-26 | 2010-09-29 | 刘学刚 | New method for ensuring quality of artemia eggs in salt lake through physiological action |
| CN102630639A (en) * | 2012-01-10 | 2012-08-15 | 李新书 | Shelling method of artemia cyst |
| CN104273100A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Brine shrimp egg shelling method for decreasing hatching rate |
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Granted publication date: 20180706 Termination date: 20190513 |