CN105746446A - Artemia cyst shelling method - Google Patents
Artemia cyst shelling method Download PDFInfo
- Publication number
- CN105746446A CN105746446A CN201610314079.4A CN201610314079A CN105746446A CN 105746446 A CN105746446 A CN 105746446A CN 201610314079 A CN201610314079 A CN 201610314079A CN 105746446 A CN105746446 A CN 105746446A
- Authority
- CN
- China
- Prior art keywords
- cysts
- shelling
- artemia
- ovum
- hatching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 208000031513 cyst Diseases 0.000 title claims abstract description 25
- 206010011732 Cyst Diseases 0.000 title abstract 2
- 241000238582 Artemia Species 0.000 title description 5
- 241001247197 Cephalocarida Species 0.000 claims abstract description 25
- 230000012447 hatching Effects 0.000 claims abstract description 14
- 102000004142 Trypsin Human genes 0.000 claims abstract description 8
- 108090000631 Trypsin Proteins 0.000 claims abstract description 8
- 239000012588 trypsin Substances 0.000 claims abstract description 8
- 239000013505 freshwater Substances 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 239000013535 sea water Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 25
- 108010000912 Egg Proteins Proteins 0.000 claims description 25
- 210000004681 ovum Anatomy 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 9
- 238000013019 agitation Methods 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 4
- 210000004080 milk Anatomy 0.000 claims description 4
- 235000013336 milk Nutrition 0.000 claims description 4
- 238000005213 imbibition Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 229930014626 natural product Natural products 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000002411 adverse Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 210000001136 chorion Anatomy 0.000 description 6
- 230000006378 damage Effects 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 4
- 125000001309 chloro group Chemical group Cl* 0.000 description 4
- 210000004379 membrane Anatomy 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 102000004895 Lipoproteins Human genes 0.000 description 2
- 108090001030 Lipoproteins Proteins 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 229940122618 Trypsin inhibitor Drugs 0.000 description 2
- 101710162629 Trypsin inhibitor Proteins 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 239000002753 trypsin inhibitor Substances 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 239000013064 chemical raw material Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000003243 intestinal obstruction Diseases 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention provides an artemia cyst shelling method. The method comprises the following steps that 1, commercial artemia cysts are soaked with fresh water so that the cysts can sufficiently absorb water and swell; 2, the artemia cysts are separated and placed in a shelling solution containing trypsin, and the mixture is slowly stirred; 3, when it is observed that the color of the cysts gradually changes from brown to orange red, a terminating agent is added; 4, standing is carried out after inflation and stirring, and shelled cysts are separated out, simply washed with sea water and then can be used for hatching or direct feeding. The shelling method is a biological shelling method, adopted raw materials are all natural products, no chemical pollution is caused to the shelled cysts, no adverse influence is imposed on direct feeding or subsequent hatching, and the effect is stable.
Description
Technical field
The invention belongs to artemia cysts applied technical field, be specifically related to a kind of method of shelling of artemia cysts.
Background technology
Artemia is named again fairy shrimp, is universal small-sized shell-fish, moves in salt lake, the contour saltwater in saltern.Artemia cysts is the aquatic fry growing bait of high-quality, has important function in culture fishery.Artemia chorion is iron content lipoprotein, and fish and shrimp seedling cannot digest, therefore it is generally required to first hatch artemia larvae, then again using germling as bait feeding.Chorion and germling after hatching are not easily completely separated, easily cause part hatched germling outwell with chorion and loss or chorion searched for food together with germling and cause fish and shrimp seedling intestinal obstruction dead.Artemia cysts can directly be thrown something and fed after shelling, it is also possible to hatches germling.Owing to there is no chorion, it is possible to reduce energy expenditure during germling broken shell, be conducive to retaining more nutrition.In fish and shrimp nursery, the expense of artemia cysts often accounts for nursery 50%-70% cost.By the method shelled, it is possible to improve incubation rate and make artemia larvae retain more nutrition, the low-quality ovum that a large amount of incubation rate is low can also be utilized, reducing seedling cost simultaneously, nursery is significant.
Current artemia cysts shells and adopts chemical method more, namely dissolves chorion with sodium hypochlorite and sodium hydroxide solution, and after shelling, reaction cannot terminate immediately, it is necessary to the ovum that will shell is pulled cleaning out and removed chlorine residue, and in this process, the chlorine residue of solution continues, to shelling ovum effect, to damage bigger；In the process of shelling, solution temperature easily raises simultaneously, affects incubation rate, it is necessary to take cooling measure；Operating process is very big on incubation rate impact, and technique is unstable；And the chlorine residue of ovum surface is difficult to thorough removal, there is chlorine distinctive smell to affect aquatic seedling when throwing something and feeding and search for food, also tend to need to add phagostimulant.
Summary of the invention
The invention provides the technical scheme that a kind of artemia cysts bioanalysis shells, it is therefore intended that solve above-mentioned chemical method shell exist deficiency.
1., by commodity artemia cysts freshwater soaking 2-4 hour, air agitation, make the abundant imbibition of ovum；
2. isolate artemia cysts, be placed on 10 times of weight and shell in liquid, 60r/min stirring at temperature 30 DEG C；
3. shelling 0.5-5 hour time, the color observing ovum is gradually become orange red by brown, adds terminator；
4. air agitation 10 minutes, stand, and separate the ovum that shells after being layered, with namely can be used for hatching after seawater flushing or directly throwing something and feeding.
If fresh water used is tap water in said process, need to dechlorinate in advance, alleviate the damage to ovum.
The described liquid composition that shells is: trypsin 0.1%-2%, EDTA0.02%, all the other are fresh water.
Described terminator composition is: Semen sojae atricolor that trypsin weight is 10 times and raw soya-bean milk prepared by the water of 5 times of Semen sojae atricolor weight.
In order to obtain effect of better shelling, in step 3, the time of shelling is preferably 2-3 hour, and the artemia of different sources time of shelling is different；Trypsin amount in liquid of shelling is preferably 0.5%-1.2%.
Lipoprotein agent structure in shell is hydrolyzed and removes by trypsin, other a small amount of remaining insoluble component in violent air agitation process by the shear action of liquid from egg membrane sur-face peeling.Containing trypsin inhibitor in Semen sojae atricolor; including Kunitz class inhibitor and BowmanBirk class inhibitor, it is possible to tryptic activity in the liquid that suppresses rapidly to shell, protection egg membrane is not damaged; use with the form of raw soya-bean milk, not only simple can also reduce cost.
Compared with prior art, beneficial effects of the present invention:
The invention belongs to bioanalysis to shell, raw materials used be natural product, do not introduce chemical raw material, to the ovum that shells without chemical contamination, follow-up hatching or directly throw something and feed is had no adverse effects, reduce operation easier, and the effect stability that shells.
Detailed description of the invention
By the examples below the present invention is described in further detail, but the present invention is not limited to following embodiment.
Embodiment 1:
1., by 500 grams of artemia cysts freshwater soaking 3 hours, air agitation, make the abundant imbibition of ovum.If fresh water used is tap water, need to dechlorinate in advance, alleviate the damage to the ovum that shells.
2. isolate artemia cysts, be placed on 5 liters and shell in liquid, 60r/min stirring at temperature 30 DEG C.The liquid composition that shells is: trypsin 0.8%, EDTA0.02%.
3. shell time 2 h, when the color of ovum is gradually become orange red by brown, adds terminator.Terminator composition is: raw soya-bean milk prepared by 400 grams of Semen sojae atricolor and 2 kg of water, and soybean trypsin inhibitor therein can effectively suppress tryptic activity, stops its destruction to egg membrane.
4. being placed in bottom solution by pneumatic head, it is tumbling state that charge flow rate reaches solution, air agitation 10 minutes, stands, and after solution is layered, the ovum that will shell is separated, and namely can be used for hatching or directly throw something and feed after simply rinsing with sea water.It is suppressed activity because of pancreatin, and the calcium ions and magnesium ions in sea water also can suppress its activity, egg membrane cannot be caused damage after diluting then through sea water, artemia larvae and fish and shrimp seedling is all harmless, save the time being largely used to repeatedly clean and artificial.
Embodiment 2: difference is shelled the method impact on artemia ovum hatching
The artemia cysts adopting the place of production to be same batch of Shandong, in natural light irradiation, salinity 30 ‰, temperature 28 DEG C, brooding time 24h, and other consistent when, take two kinds of methods incubation rate at shelling rate corresponding to different disposal time and the ovum that shells accordingly, contrasting the method for the shelling two kinds different impact on artemia ovum hatching, result is as shown in table 1.Wherein: matched group is normal incubation, does not shell；Chemical method is sodium hypochlorite and sodium hydroxide coupling；The incubation rate of two kinds of methods of shelling is the egg hatching rate that shells.
The impact on artemia ovum hatching of the table 1 different disposal method
Using chemical method to shell in process, the incubation rate of the ovum that shells becomes negative correlation with shelling rate, and the time of shelling, more long then shelling rate was more high, and incubation rate is more low.Although the egg hatching rate that shells when shelling rate is low is higher than matched group, but substantial portion of ovum is now also had not shell, so the overall incubation rate of ovum improves less；When shelling completely, overall incubation rate is lower than normal incubation on the contrary.Employing the inventive method is shelled, action temperature and, to ovum damage little, the egg hatching rate that shells when shelling rate height is still higher, makes the overall incubation rate of ovum improve a lot, and directly throw something and feed or for hatching all suitable.
Claims (2)
1. the method for shelling of an artemia cysts, it is characterised in that: comprise the steps,
(1) by commodity artemia cysts freshwater soaking 2-4 hour, air agitation, the abundant imbibition of ovum is made；
(2) isolate artemia cysts, be placed on 10 times of weight and shell in liquid, 60r/min stirring at temperature 30 DEG C；
(3) shell 0.5-5 hour time, when the color of observation ovum is gradually become orange red by brown, add terminator；
(4) air agitation 10 minutes, stand, isolate the ovum that shells after being layered, with namely can be used for hatching after seawater flushing or directly throwing something and feeding.
2. the method for shelling of artemia cysts according to claim 1, it is characterised in that: the liquid composition that shells in step (2) is: trypsin 0.1%-2%, EDTA0.02%；In step (3), terminator composition is: Semen sojae atricolor that trypsin weight is 10 times and raw soya-bean milk prepared by the water of 5 times of Semen sojae atricolor weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610314079.4A CN105746446B (en) | 2016-05-13 | 2016-05-13 | A kind of decladding method of artemia eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610314079.4A CN105746446B (en) | 2016-05-13 | 2016-05-13 | A kind of decladding method of artemia eggs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105746446A true CN105746446A (en) | 2016-07-13 |
| CN105746446B CN105746446B (en) | 2018-07-06 |
Family
ID=56322890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610314079.4A Expired - Fee Related CN105746446B (en) | 2016-05-13 | 2016-05-13 | A kind of decladding method of artemia eggs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105746446B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
| CN110055213A (en) * | 2019-04-23 | 2019-07-26 | 中国海洋大学 | A kind of separation method of dwarf clam egg membrane |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843237A (en) * | 2009-03-26 | 2010-09-29 | 刘学刚 | New method for ensuring quality of artemia eggs in salt lake through physiological action |
| CN102630639A (en) * | 2012-01-10 | 2012-08-15 | 李新书 | Shelling method of artemia cyst |
| CN104273100A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Brine shrimp egg shelling method for decreasing hatching rate |
-
2016
- 2016-05-13 CN CN201610314079.4A patent/CN105746446B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843237A (en) * | 2009-03-26 | 2010-09-29 | 刘学刚 | New method for ensuring quality of artemia eggs in salt lake through physiological action |
| CN102630639A (en) * | 2012-01-10 | 2012-08-15 | 李新书 | Shelling method of artemia cyst |
| CN104273100A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Brine shrimp egg shelling method for decreasing hatching rate |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN106577408B (en) * | 2016-11-22 | 2019-09-03 | 江苏好润生物科技有限公司 | It is a kind of for improving the preparation method of the shelling slag oxygenation of hatching rate |
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
| CN110055213A (en) * | 2019-04-23 | 2019-07-26 | 中国海洋大学 | A kind of separation method of dwarf clam egg membrane |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105746446B (en) | 2018-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Treece | Artemia production for marine larval fish culture | |
| CN102630639A (en) | Shelling method of artemia cyst | |
| EP2916664B1 (en) | Marine material derived from early developmental stages of barnacles | |
| CN105746446A (en) | Artemia cyst shelling method | |
| Tanaka | Techniques for larval rearing | |
| CN104273100A (en) | Brine shrimp egg shelling method for decreasing hatching rate | |
| CN107079843B (en) | Breeding method of coregonus ussuriensis fries | |
| CN106577408B (en) | It is a kind of for improving the preparation method of the shelling slag oxygenation of hatching rate | |
| CN105900934B (en) | Application of the papain in terms of artemia eggs decladding | |
| CN106508824A (en) | Pretreatment method for efficiently improving ova hatching rate in good year | |
| CN103563860A (en) | Method for increasing hatching rate of diapause artemia eggs in weak light environment | |
| EP1575353A1 (en) | Process for decapsulating crustacean or rotifer eggs | |
| CN103749344A (en) | Artificial breeding method for paddlefish | |
| CN105995653A (en) | Method for preparing artemia-cysts-shell hydrolyzing liquid | |
| Ottesen et al. | Values from waste | |
| Rahman et al. | A training manual on Artemia cyst hatching and decapsulation | |
| CN103096729A (en) | Food for aquacultural feeding | |
| JP2019187405A (en) | Breeding feed for seafood and production method | |
| JP6997342B2 (en) | How to produce live aquaculture feed | |
| Perkins et al. | Recovery and application of organic wastes from the Louisiana shrimp canning industry | |
| Moreno-Reyes et al. | Optimization of techniques and procedures for the production and management of live feed used in the larval culture of the freshwater shrimp Cryphiops caementarius (Decapoda: Palaemonidae) | |
| CN113678956A (en) | Preparation method and feeding method of mixed feed for sea spurs jellyfish | |
| RU1784146C (en) | Method for decapsulating the eggs of crustacean acfemia salina sp | |
| CN112322545A (en) | Parabacteroides dieldii LCG-06, compound microbial inoculum compounded with bile acid and application thereof | |
| CN102630663A (en) | Method for storing shelled brine shrimp eggs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180706 Termination date: 20190513 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |