CN104273100A - Brine shrimp egg shelling method for decreasing hatching rate - Google Patents
Brine shrimp egg shelling method for decreasing hatching rate Download PDFInfo
- Publication number
- CN104273100A CN104273100A CN201410563499.7A CN201410563499A CN104273100A CN 104273100 A CN104273100 A CN 104273100A CN 201410563499 A CN201410563499 A CN 201410563499A CN 104273100 A CN104273100 A CN 104273100A
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- Prior art keywords
- shelling
- eggs
- slag oxygenation
- shelled
- water
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- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000012447 hatching Effects 0.000 title abstract description 13
- 241000238426 Anostraca Species 0.000 title abstract description 10
- 230000003247 decreasing effect Effects 0.000 title abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 239000013505 freshwater Substances 0.000 claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 238000006298 dechlorination reaction Methods 0.000 claims abstract description 4
- 238000006213 oxygenation reaction Methods 0.000 claims description 58
- 239000002893 slag Substances 0.000 claims description 58
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 230000036571 hydration Effects 0.000 claims description 20
- 238000006703 hydration reaction Methods 0.000 claims description 20
- 238000011534 incubation Methods 0.000 claims description 18
- 102000002322 Egg Proteins Human genes 0.000 claims description 17
- 108010000912 Egg Proteins Proteins 0.000 claims description 17
- 210000004681 ovum Anatomy 0.000 claims description 17
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 8
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 claims description 6
- 238000005273 aeration Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 abstract description 8
- 235000013601 eggs Nutrition 0.000 abstract 15
- 238000005406 washing Methods 0.000 abstract 3
- 230000000887 hydrating effect Effects 0.000 abstract 2
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 abstract 2
- 235000019345 sodium thiosulphate Nutrition 0.000 abstract 2
- 241000675108 Citrus tangerina Species 0.000 abstract 1
- 241000238582 Artemia Species 0.000 description 7
- 210000001136 chorion Anatomy 0.000 description 7
- 241000133262 Nauplius Species 0.000 description 4
- 208000031513 cyst Diseases 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 3
- 150000002367 halogens Chemical class 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000003243 intestinal obstruction Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a brine shrimp egg shelling method for decreasing the hatching rate. The method includes the steps of 1) hydrating brine shrimp eggs for 1-3 hours, wherein the salinity of hydrating water is 0-3%; 2) after the brine shrimp eggs absorb water sufficiently, transferring the brine shrimp eggs to shelling liquid and stirring and aerating continuously; 3) enabling the shelling time of the brine shrimp eggs in the shelling liquid to be 5-15 minutes, wherein shelling is complete when the color of the brine shrimp eggs is changed from brown to grey white and to tangerine finally; 4) after shelling is completed, collecting shelled eggs by a 200-mesh screen net and washing the shelled eggs along the edge of a net bag by a great quantity of fresh water, so that damage to the eggs by direct washing is prevented; 5) after the shelled eggs are washed by the fresh water, the washed shelled eggs are washed by sodium thiosulfate solution, or immersing the washed shelled eggs into the sodium thiosulfate solution for dechlorination for 1-2 minutes prior to washing the dechlorinated shelled eggs by the fresh water, so that the shelled brine shrimp eggs are obtained. The hatching rate of the shelled brine shrimp eggs obtained by the method is increased substantially and is up to 86%.
Description
Technical field
The present invention relates to the hatching technique field of slag oxygenation, particularly relating to a kind of method of the slag oxygenation shelling for improving incubation rate.
Background technology
Halogen worm (Brine shrimp, Artemia), be commonly called as fairy shrimp, it is the small-sized shellfish that a class lives in high salinity waters, the nauplius hatching the halogen worm of 1 ~ 2d has a large amount of yolk, containing rich in protein, fat, is the good open-mouthed bait of the young such as fish shrimp crab, greatly can improve fishes and shrimps artificial breeding survival rate, but not polluted water.
Along with the development of aquatic products industry, the young and the adult of halogen worm more and more receive an acclaim.After artemia ovum hatching, Artemia nauplii and chorion and do not hatch worm's ovum and mix, need at the beginning of incubate nauplius from wherein separating, this not only needs manpower and materials, and not yet have simple on producing, the highly effective method of nauplius can be separated completely.Thus, when throwing something and feeding, just inevitably part chorion is had to be thrown something and fed in nursery pond together with nauplius with unhatched ovum.These chorions and unhatched ovum, in fry pond, can rot on the one hand, directly ruin water quality, or because of on chorion with a large amount of bacteriums, fungi and cause water pollution or disease to occur; On the other hand, some cultivation object is eaten chorion or can cause intestinal obstruction after not hatching ovum, even dead.Therefore, chorion should be removed.
Shelling of slag oxygenation is exactly by the outermost layer of chorion (crust layer) oxidation removal.Sorgeloos et al (1977) reports the chemical decladding method of Artemia cysts the earliest.Slag oxygenation after shelling can directly be thrown something and fed, and also can be used for hatching process further.The method but existing slag oxygenation shells, makes the hatchability of artemia cysts after shelling reduce, and not yet someone proposes how slag oxygenation to be carried out process of shelling, and could improve the incubation rate of slag oxygenation.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of method of the slag oxygenation shelling for improving incubation rate, shortens the time of slag oxygenation shelling, cost-saving, and avoids the lower phenomenon of the hatchability of artemia cysts after shelling.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of method of the slag oxygenation shelling for improving incubation rate, comprises following step,
1), by slag oxygenation hydration 1 ~ 3h, the salinity of hydration water is 0-30 ‰;
2), after slag oxygenation fully absorbs water, transferred to and shelled in liquid, not to stirring inflation;
3), slag oxygenation is 5 ~ 15min in the shelling time in liquid of shelling, and the color of slag oxygenation becomes canescence from brown, and the slag oxygenation of last 65 ~ 75% is Chinese red, then terminate to shell; The temperature of liquid of shelling controls below 10 DEG C, and flow of aerating air is 75-150m
3/ (m
2min); The liquid that shells is formulated by clorox, sodium hydroxide and water, and wherein the mass values of clorox and water is 1:2.5-1:5, and the amount of sodium hydroxide is according to every gram of dry slag oxygenation 0.14-0.17 gram of calculating;
4), shelled after, collect with 200 eye mesh screens and to shell ovum, use a large amount of fresh water flush; Rinse along the string bag edge during flushing, to prevent from flinging the damage to ovum;
5), fresh water flush shells after ovum, rinses, or immerses dechlorination 1 ~ 2min in hypo solution, then use fresh water flush, namely obtain the slag oxygenation after shelling with hypo solution.
Preferably, step 1) in, in the process of slag oxygenation hydration, inflate hydration water, flow of aerating air is 75-150m
3/ (m
2min).
Preferably, step 5) described in the mass concentration of hypo solution be 1.5%.
Preferably, step 1) in hydration water, step 2) in shell liquid, step 4) and step 5) in fresh water are all in advance aeration 1 ~ 2d.
The advantage that the present invention has and good effect are: use the method that slag oxygenation provided by the invention shells, and the incubation rate of the slag oxygenation of shelling increases substantially, and reaches 86%.
Embodiment
Embodiment one
For improving a method for the slag oxygenation shelling of incubation rate, comprise following step,
1), by slag oxygenation hydration 1h, the salinity of hydration water is 20 ‰, in the process of slag oxygenation hydration, inflates hydration water, and flow of aerating air is 95m
3/ (m
2min);
2), after slag oxygenation fully absorbs water, transferred to and shelled in liquid, not to stirring inflation;
3), slag oxygenation is 10min in the shelling time in liquid of shelling, and the color of slag oxygenation becomes canescence from brown, and the slag oxygenation of last 70% is Chinese red, then terminate to shell; The temperature of liquid of shelling controls below 10 DEG C, and flow of aerating air is 90m
3/ (m
2min); The liquid that shells is formulated by clorox, sodium hydroxide and water, and wherein the mass values of clorox and water is 1:5, and the amount of sodium hydroxide is according to every gram of dry slag oxygenation 0.15 gram calculating;
4), shelled after, collect with 200 eye mesh screens and to shell ovum, use a large amount of fresh water flush; Rinse along the string bag edge during flushing, to prevent from flinging the damage to ovum;
5), fresh water flush shells after ovum, rinses, then uses fresh water flush, namely obtain the slag oxygenation after shelling with the hypo solution that mass concentration is 1.5%.
Step 1) in hydration water, step 2) in shell liquid, step 4) and step 5) in fresh water are all in advance aeration 1d.
After the artemia ovum hatching 24h of shelling, incubation rate is 86%.
The incubation condition of slag oxygenation be salinity 20g dissolving salt/liter; PH value 8.1; Oxygen concentration > 6mg/L; Water temperature 28 DEG C, the intensity of illumination of white light is 1000lux.
Embodiment two
Identical with the method step of embodiment one, by step 3) in the shelling time change 15min into, the slag oxygenation of last 75% is Chinese red, and the slag oxygenation of shelling is carried out hatching 24h under the condition identical with embodiment one, and incubation rate is 70%.
Embodiment three
For improving a method for the slag oxygenation shelling of incubation rate, comprise following step,
1), by slag oxygenation hydration 1h, the salinity of hydration water is 20 ‰; In the process of slag oxygenation hydration, inflate hydration water, flow of aerating air is 95m
3/ (m
2min).
2), after slag oxygenation fully absorbs water, transferred to and shelled in liquid, not to stirring inflation;
3), slag oxygenation is 5min in the shelling time in liquid of shelling, and the color of slag oxygenation becomes canescence from brown, and the slag oxygenation of last 65% is Chinese red, then terminate to shell; The temperature of liquid of shelling controls below 10 DEG C, and flow of aerating air is 90m
3/ (m
2min); The liquid that shells is formulated by clorox, sodium hydroxide and water, and wherein the mass values of clorox and water is 1:2.5, and the amount of sodium hydroxide is according to every gram of dry slag oxygenation 0.15 gram calculating;
4), shelled after, collect with 200 eye mesh screens and to shell ovum, use a large amount of fresh water flush; Rinse along the string bag edge during flushing, to prevent from flinging the damage to ovum;
5), fresh water flush shells after ovum, immerses dechlorination 2min in hypo solution, then uses fresh water flush, namely obtain the slag oxygenation after shelling.
Step 1) in hydration water, step 2) in shell liquid, step 4) and step 5) in fresh water are all in advance aeration 2d.
The slag oxygenation of shelling is carried out hatching 24h under the condition identical with embodiment one, and incubation rate is 84%.
Embodiment four
Identical with the method step of embodiment one, by step 3) in the shelling time change 11min into, the slag oxygenation of last 72% is Chinese red, and the slag oxygenation of shelling is carried out hatching 24h under the condition identical with embodiment one, and incubation rate is 85%.
Control group
To not carry out the slag oxygenation of heat treatment, under the condition identical with embodiment one, carry out hatching 24h, incubation rate is 62%.
Above embodiments of the invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the scope of the invention change and improve, and all should still belong within this patent covering scope.
Claims (4)
1., for improving a method for the slag oxygenation shelling of incubation rate, it is characterized in that: comprise following step,
1), by slag oxygenation hydration 1 ~ 3h, the salinity of hydration water is 0-30 ‰;
2), after slag oxygenation fully absorbs water, transferred to and shelled in liquid, not to stirring inflation;
3), slag oxygenation is 5 ~ 15min in the shelling time in liquid of shelling, and the color of slag oxygenation becomes canescence from brown, and the slag oxygenation of last 65 ~ 75% is Chinese red, then terminate to shell; The temperature of liquid of shelling controls below 10 DEG C, and flow of aerating air is 75-150m
3/ (m
2min); The liquid that shells is formulated by clorox, sodium hydroxide and water, and wherein the mass values of clorox and water is 1:2.5-1:5, and the amount of sodium hydroxide is according to every gram of dry slag oxygenation 0.14-0.17 gram of calculating;
4), shelled after, collect with 200 eye mesh screens and to shell ovum, use a large amount of fresh water flush;
5), fresh water flush shells after ovum, rinses, or immerses dechlorination 1 ~ 2min in hypo solution, then use fresh water flush, namely obtain the slag oxygenation after shelling with hypo solution.
2. the method for the slag oxygenation shelling for improving incubation rate according to claim 1, is characterized in that: step 1) in, in the process of slag oxygenation hydration, inflate hydration water, flow of aerating air is 75-150m
3/ (m
2min).
3. the method for the slag oxygenation shelling for improving incubation rate according to claim 1 and 2, is characterized in that: step 5) described in the mass concentration of hypo solution be 1.5%.
4. the method for the slag oxygenation shelling for improving incubation rate according to claim 3, is characterized in that: step 1) in hydration water, step 2) in shell liquid, step 4) and step 5) in fresh water are all in advance aeration 1 ~ 2d.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410563499.7A CN104273100A (en) | 2014-10-21 | 2014-10-21 | Brine shrimp egg shelling method for decreasing hatching rate |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746446A (en) * | 2016-05-13 | 2016-07-13 | 辽宁石油化工大学 | Artemia cyst shelling method |
| CN105900934A (en) * | 2016-05-13 | 2016-08-31 | 辽宁石油化工大学 | Application of papain to artemia cyst shell removing |
| CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN106954593A (en) * | 2017-04-14 | 2017-07-18 | 南京农业大学 | A kind of white light illumination method of leech indoor highly effective cultivation |
| CN107049923A (en) * | 2017-05-07 | 2017-08-18 | 辽宁石油化工大学 | A kind of preparation method of artemia cysts product |
| CN107079843A (en) * | 2017-05-02 | 2017-08-22 | 中国水产科学研究院黑龙江水产研究所 | Coregonus ussuriensis offspring seed cultivation method |
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
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| CN102630639A (en) * | 2012-01-10 | 2012-08-15 | 李新书 | Shelling method of artemia cyst |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746446A (en) * | 2016-05-13 | 2016-07-13 | 辽宁石油化工大学 | Artemia cyst shelling method |
| CN105900934A (en) * | 2016-05-13 | 2016-08-31 | 辽宁石油化工大学 | Application of papain to artemia cyst shell removing |
| CN105900934B (en) * | 2016-05-13 | 2019-01-25 | 辽宁石油化工大学 | Application of the papain in terms of artemia eggs decladding |
| CN106577408A (en) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | Decapsulated artemia egg preparation method improving hatching rate |
| CN106577408B (en) * | 2016-11-22 | 2019-09-03 | 江苏好润生物科技有限公司 | It is a kind of for improving the preparation method of the shelling slag oxygenation of hatching rate |
| CN106508824A (en) * | 2016-12-15 | 2017-03-22 | 雷州市海威水产养殖有限公司 | Pretreatment method for efficiently improving ova hatching rate in good year |
| CN106954593A (en) * | 2017-04-14 | 2017-07-18 | 南京农业大学 | A kind of white light illumination method of leech indoor highly effective cultivation |
| CN107079843A (en) * | 2017-05-02 | 2017-08-22 | 中国水产科学研究院黑龙江水产研究所 | Coregonus ussuriensis offspring seed cultivation method |
| CN107049923A (en) * | 2017-05-07 | 2017-08-18 | 辽宁石油化工大学 | A kind of preparation method of artemia cysts product |
| CN107347872A (en) * | 2017-06-30 | 2017-11-17 | 天津海友佳音生物科技股份有限公司 | A kind of artemia shelling egg freezing preserves and the formula and method of hatching |
| CN107347872B (en) * | 2017-06-30 | 2021-11-19 | 天津海友佳音生物科技股份有限公司 | Formula and method for freezing, storing and hatching artemia unshelled eggs |
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Application publication date: 20150114 |