CN102048056A - Octopus larva initial baits and production method thereof - Google Patents

Octopus larva initial baits and production method thereof Download PDF

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Publication number
CN102048056A
CN102048056A CN201010519225XA CN201010519225A CN102048056A CN 102048056 A CN102048056 A CN 102048056A CN 201010519225X A CN201010519225X A CN 201010519225XA CN 201010519225 A CN201010519225 A CN 201010519225A CN 102048056 A CN102048056 A CN 102048056A
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octopus
wheel animalcule
hours
artemia nauplii
liquid
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CN201010519225XA
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吴常文
迟长凤
徐佳晶
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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Abstract

The invention discloses octopus larva initial baits and a production method thereof. The octopus larva initial baits provided by the invention contain wheel animalcules, artemia nauplii and euglena gracilis, wherein the number ratio of the wheel animalcules to the artemia nauplii is 9: 1 to 1: 9; and the number ratio of the euglena gracilis to the wheel animalcules and the artemia nauplii is 40: 1 to 55: 1. The ratio of the wheel animalcules to the artemia is adjusted according to the octopus larva cultivating time, so the overall mouth opening efficiency of the octopus larva can be improved; and part of octopus larvae which develop slowly are compensated with a small amount of wheel animalcules for further cultivation. The production method for the octopus larva initial baits comprises: (1) a step of strengthening the euglena gracilis; (2) a step of cultivating bait microbes; and (3) a step of mixing. The produced baits have long activation time and are tasteful, so the mass octopus larva production can be carried out under artificial conditions, the survival rate of the octopus larvae can be improved, and the octopus larvae have uniform feeding amount after opening mouth.

Description

Octopus young open-mouthed bait and production method thereof
Technical field
The present invention relates to a kind of aquatic product sprout open-mouthed bait and production method thereof, particularly octopus young open-mouthed bait and production method thereof.
Background technology
The octopus major part is a shallow sea property kind, and minority deep-sea property kind is also arranged.The octopus inner casing is degenerated, and little shell pin on both sides, back residual two is not only had a photophore.1 pair of fallopian tubal of female body tool.Main battalion dwells at the end and lives in the seabed and creep, and also can rely on of short duration the parade in the bottom seawater of reaction of funnel water spray.Have short square from reproduction and overwintering migration habit, be food with lobster, shrimp aunt, crab class, shellfish and benthic fishes.Self is the hunting object of shark, conger pile etc. often.Different types of egg laying amount of octopus differs very big, from several thousand surplus ten ten thousand do not wait, ovum is ripe in batches, output in batches, the ovum shape such as the rice grain of output, Chang Chengsui connects together.The object that octopus is fished for as the coastal waters tradition, because otter-trawl is fished for bosom ovum parent in its trip in a large number to offshore in reproduction period, made resource be subjected to very big infringement, to the nineties in 20th century, fishing operation in coastal each the main octopus fishing ground of China obviously reduces, and its stock number falls sharply.In the seed of fish produces, understand fully the selectivity of young postlarva to food organisms, significant to rationally effectively utilizing existing bait, develop new food organisms and improving nursery effect.Especially the early stage prelarva initial feeding stage is the critical period of fish early development, and the phenomenon of prelarva mortality often takes place, and lacking sufficient agreeable to the taste exogenous bait is to cause one of prelarva main causes of death, thereby has influenced the development of aquaculture.The enforcement of the artificial large-scale farming of octopus, to increasing the sea-farming new varieties, it is significant to increase economic efficiency, and to the open-mouthed bait technology of the octopus young, also not have the document of being correlated with disclosed.
Summary of the invention
The purpose of this invention is to provide a kind of octopus young open-mouthed bait and production method thereof, provide long and agreeable to the taste exogenous octopus young bait of active retention time with this, realize that the extensive octopus young produces under the artificial condition, and this kind bait can improve the survival rate of the octopus young, the octopus young uniform specification of ingesting behind the opening, the production method of this bait is simple, is suitable for large-scale production.
The present invention is achieved in that octopus young open-mouthed bait, and it contains wheel animalcule, artemia nauplii, very thin Euglena; Wheel animalcule and artemia nauplii are 9: 1 to 1: 9 according to the number ratio; Very thin Euglena and wheel animalcule and artemia nauplii are 40: 1 to 55: 1 according to the number ratio.
The optimization measure of taking comprises: wheel animalcule, artemia nauplii were all used very thin Euglena hot housing 4 hours to 9 hours; Very thin Euglena is adopted the hot housing of n-3HUFA reinforced solution, and the mass ratio of the DHA of n-3HUFA reinforced solution and EPA is 3: 1 to 4: 1.
Octopus young open-mouthed bait production method is characterized in that comprising the steps:
1. very thin Euglena is strengthened step: use the seawater through sedimentation and filtration, scalding to add the n-3HUFA reinforced solution, inoculate very thin Euglena algae kind then;
2. food organisms incubation step:
A, wheel animalcule are cultivated: choose very thin Euglena liquid and the inoculating rotifer 1. handled through step, cultivated 8 hours to 10 hours, the elimination seawater gets wheel animalcule liquid;
B, artemia nauplii are cultivated: choose very thin Euglena liquid and the inoculating rotifer 1. handled through step, cultivated 5 hours to 7 hours, the elimination seawater gets halogen worm liquid;
3. blend step: wheel animalcule liquid and halogen worm liquid that 2. step is obtained mix in proportion.Ratio hatches pond fate adjustment according to the octopus young; The octopus young went out the pond 3 days, and wheel animalcule and artemia nauplii are 9: 1 according to the number ratio; Every increase by one day, the wheel animalcule ratio descends 1, and the artemia nauplii ratio rises 1.
For further optimization, the technical measures of taking also comprise: step 1. condition of culture is: temperature is 23~25 ℃, continuous illumination, and intensity is 2000lx to 3000lx, and salinity is 20 ‰ to 30 ‰, and pH is 7.0~8.3, the little inflation of gas stone, stopping density is 4 * 10 4C/ml to 5.5 * 10 4C/ml cultivated 4 hours to 9 hours, obtained very thin Euglena liquid;
The step 1. DHA in the n-3HUFA reinforced solution and the mass ratio of EPA is 3: 1 to 4: 1, and the total amount of DHA and EPA accounts for more than 65% of n-3HUFA reinforced solution mass fraction.
Step 1. n-3HUFA reinforced solution accounts for an one thousandth of seawater volume.
Compared with prior art, the invention has the advantages that:
Octopus young open-mouthed bait of the present invention contains wheel animalcule, artemia nauplii, very thin Euglena; Wheel animalcule and artemia nauplii are 9: 1 to 1: 9 according to the number ratio; Very thin Euglena and wheel animalcule and artemia nauplii are 40: 1 to 55: 1 according to the number ratio.It adjusts the proportioning of wheel animalcule, halogen worm according to octopus young incubation time, can improve the aperture efficiency of octopus young integral body, the octopus young to bradyauxesis compensates with a small amount of wheel animalcule, when finishing the young opening during production phase, can make most octopus young transfer the halogen worm to from the wheel animalcule that ingests, reach the ingest effect of uniform specification of the octopus young behind the opening, be beneficial to further cultivation.Very thin Euglena and wheel animalcule and artemia nauplii are 40: 1 to 55: 1 according to the number ratio, can keep wheel animalcule and halogen worm that feeding behaviour was still arranged in long period of time in the time at the end after the operation of throwing something and feeding, and keep strong vigor.Octopus young open-mouthed bait production method of the present invention adopts 1. very thin Euglena reinforcement step, 2. the food organisms incubation step reaches 3. blend step, the active retention time of the bait that it is produced is long and agreeable to the taste, can carry out the octopus young under artificial condition on a large scale produces, improve the survival rate of the octopus young, the octopus young uniform specification of ingesting behind the opening.
The specific embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Embodiment: octopus young open-mouthed bait, it contains wheel animalcule, artemia nauplii, very thin Euglena; Wheel animalcule and artemia nauplii are 9: 1 to 1: 9 according to the number ratio; Very thin Euglena and wheel animalcule and artemia nauplii are 40: 1 to 55: 1 according to the number ratio.
The optimization measure of taking comprises: wheel animalcule, artemia nauplii were all used very thin Euglena hot housing 4 hours to 9 hours; Very thin Euglena is adopted the hot housing of n-3HUFA reinforced solution, and the mass ratio of the DHA of n-3HUFA reinforced solution and EPA is 3: 1 to 4: 1.
Octopus young open-mouthed bait production method is characterized in that comprising the steps:
1. very thin Euglena is strengthened step: use the seawater through sedimentation and filtration, scalding to add the n-3HUFA reinforced solution, inoculate very thin Euglena algae kind then; Cultivating temperature is 24 ℃, continuous illumination, and intensity is 3000lx, and salinity is 22 ‰, and pH is 7.2, the little inflation of gas stone, stopping density is 5.5 * 10 4C/ml, incubation was finished in 7 hours, obtained very thin Euglena liquid;
DHA in the n-3HUFA reinforced solution and the mass ratio of EPA are 3: 1, and the total amount of DHA and EPA accounts for more than 65% of n-3HUFA reinforced solution mass fraction, and present embodiment is 73%.
The n-3HUFA reinforced solution accounts for an one thousandth of seawater volume.
2. food organisms incubation step:
A, wheel animalcule are cultivated: choose very thin Euglena liquid and the inoculating rotifer 1. handled through step, cultivated 8 hours to 10 hours, the elimination seawater gets wheel animalcule liquid;
B, artemia nauplii are cultivated: choose very thin Euglena liquid and the inoculating rotifer 1. handled through step, cultivated 5 hours to 7 hours, the elimination seawater gets halogen worm liquid;
3. blend step: wheel animalcule liquid and halogen worm liquid that 2. step is obtained mix in proportion.Ratio hatches pond fate adjustment according to the octopus young; The octopus young went out the pond 3 days, and wheel animalcule and artemia nauplii are 9: 1 according to the number ratio; Every increase by one day, the wheel animalcule ratio descends 1, and the artemia nauplii ratio rises 1.Promptly hatching the pond the 3rd day the octopus young, is 9: 1 ratio proportioning wheel animalcule liquid and halogen worm liquid according to the number ratio with wheel animalcule and artemia nauplii.Thereby, before proportioning, should count wheel animalcule liquid and halogen worm liquid respectively.Hatching the pond the 4th day the octopus young, is 8: 2 ratio proportioning wheel animalcule liquid and halogen worm liquid with wheel animalcule and artemia nauplii according to the number ratio, and the like.Until aforementioned proportion to 1: till 9, finish young opening.
Although described the present invention in conjunction with preferred embodiment; right its is not in order to limit the present invention; any those skilled in the art; under the situation that does not break away from the spirit and scope of the present invention; can implement the displacement and the modification of various changes, coordinate to the theme of listing here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that is proposed.

Claims (6)

1. octopus young open-mouthed bait is characterized in that: contain wheel animalcule, artemia nauplii, very thin Euglena; Described wheel animalcule and artemia nauplii are 9: 1 to 1: 9 according to the number ratio; Described very thin Euglena and described wheel animalcule and artemia nauplii are 40: 1 to 55: 1 according to the number ratio.
2. octopus young open-mouthed bait according to claim 1 is characterized in that: described wheel animalcule, artemia nauplii were all used very thin Euglena hot housing 4 hours to 9 hours; Described very thin Euglena is adopted the hot housing of n-3HUFA reinforced solution, and the mass ratio of the DHA of described n-3HUFA reinforced solution and EPA is 3: 1 to 4: 1.
3. octopus young open-mouthed bait production method is characterized in that comprising the steps:
1. very thin Euglena is strengthened step: use the seawater through sedimentation and filtration, scalding to add the n-3HUFA reinforced solution, inoculate very thin Euglena algae kind then;
2. food organisms incubation step:
A, wheel animalcule are cultivated: choose very thin Euglena liquid and the inoculating rotifer 1. handled through step, cultivated 8 hours to 10 hours, the elimination seawater gets wheel animalcule liquid;
B, artemia nauplii are cultivated: choose very thin Euglena liquid and the inoculating rotifer 1. handled through step, cultivated 5 hours to 7 hours, the elimination seawater gets halogen worm liquid;
3. blend step: wheel animalcule liquid and halogen worm liquid that 2. step is obtained mix in proportion.Described ratio hatches pond fate adjustment according to the octopus young; The octopus young went out the pond 3 days, and wheel animalcule and artemia nauplii are 9: 1 according to the number ratio; Every increase by one day, the wheel animalcule ratio descends 1, and the artemia nauplii ratio rises 1.
4. octopus young open-mouthed bait production method according to claim 2, it is characterized in that: step 1. condition of culture is: temperature is 23~25 ℃, continuous illumination, intensity is 2000lx to 3000lx, and salinity is 20 ‰ to 30 ‰, and pH is 7.0~8.3, the little inflation of gas stone, stopping density is 4 * 10 4C/ml to 5.5 * 10 4C/ml cultivated 4 hours to 9 hours, obtained very thin Euglena liquid.
5. octopus young open-mouthed bait production method according to claim 3, it is characterized in that: DHA in the 1. described n-3HUFA reinforced solution of step and the mass ratio of EPA are 3: 1 to 4: 1, and the total amount of DHA and EPA accounts for more than 65% of n-3HUFA reinforced solution mass fraction.
6. octopus young open-mouthed bait production method according to claim 4 is characterized in that: the 1. described n-3HUFA reinforced solution of step accounts for an one thousandth of described seawater volume.
CN201010519225XA 2010-10-22 2010-10-22 Octopus larva initial baits and production method thereof Pending CN102048056A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657120A (en) * 2012-05-09 2012-09-12 中国海洋大学 Method for breeding pelagic-period larva of octopus vulgaris in aerial fog manner
CN103858815A (en) * 2012-12-18 2014-06-18 上海市水产研究所 Method for ecologically cultivating rotifers in summer eastern puffer fish fingerling culture pond
CN104431631A (en) * 2014-12-30 2015-03-25 浙江海洋学院 Grass carp bait
CN104472448A (en) * 2014-12-30 2015-04-01 浙江海洋学院 Bait for crucian carp
CN105724828A (en) * 2016-02-23 2016-07-06 北海利洋苗种繁育有限公司 Nutrient enhancing and producing method of fairy shrimp nauplius
CN106841519A (en) * 2016-12-30 2017-06-13 浙江海洋大学 A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application
CN107455615A (en) * 2017-07-21 2017-12-12 浙江海洋大学 A kind of artemia fortification method suitable for true octopus floating larva culture
CN109463318A (en) * 2018-11-08 2019-03-15 莆田市水产科学研究所 A kind of breeding method of the true octopus young

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* Cited by examiner, † Cited by third party
Title
《上海水产大学学报》 19991231 华雪铭等 温度和光照对微藻的生长、总脂肪含量及脂肪酸组成的影响 第8卷, 第04期 *
《福建水产》 19940331 林雅弘等 纤细裸藻及其对轮虫、卤虫的营养强化 , 第01期 *
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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657120A (en) * 2012-05-09 2012-09-12 中国海洋大学 Method for breeding pelagic-period larva of octopus vulgaris in aerial fog manner
CN103858815A (en) * 2012-12-18 2014-06-18 上海市水产研究所 Method for ecologically cultivating rotifers in summer eastern puffer fish fingerling culture pond
CN103858815B (en) * 2012-12-18 2015-09-09 上海市水产研究所 A kind of interior ecological method of cultivating wheel animalcule of Fugu fingerling culturing pool in summer
CN104431631A (en) * 2014-12-30 2015-03-25 浙江海洋学院 Grass carp bait
CN104472448A (en) * 2014-12-30 2015-04-01 浙江海洋学院 Bait for crucian carp
CN105724828A (en) * 2016-02-23 2016-07-06 北海利洋苗种繁育有限公司 Nutrient enhancing and producing method of fairy shrimp nauplius
CN106841519A (en) * 2016-12-30 2017-06-13 浙江海洋大学 A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application
CN107455615A (en) * 2017-07-21 2017-12-12 浙江海洋大学 A kind of artemia fortification method suitable for true octopus floating larva culture
CN107455615B (en) * 2017-07-21 2020-05-22 浙江海洋大学 Artemia nutrition strengthening method suitable for culturing octopus ocellatus planktonic larvae
CN109463318A (en) * 2018-11-08 2019-03-15 莆田市水产科学研究所 A kind of breeding method of the true octopus young
CN109463318B (en) * 2018-11-08 2021-04-09 莆田市水产科学研究所 Cultivation method of octopus ocellatus larvae

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Application publication date: 20110511