CN106841519A - A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application - Google Patents
A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application Download PDFInfo
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- CN106841519A CN106841519A CN201611251157.7A CN201611251157A CN106841519A CN 106841519 A CN106841519 A CN 106841519A CN 201611251157 A CN201611251157 A CN 201611251157A CN 106841519 A CN106841519 A CN 106841519A
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- 241000282376 Panthera tigris Species 0.000 title claims abstract description 70
- 241000238371 Sepiidae Species 0.000 title claims abstract description 70
- 238000012216 screening Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 27
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 21
- 230000035764 nutrition Effects 0.000 title claims abstract description 21
- 239000002253 acid Substances 0.000 claims abstract description 34
- 241001247197 Cephalocarida Species 0.000 claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 19
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 14
- 229930195729 fatty acid Natural products 0.000 claims abstract description 14
- 239000000194 fatty acid Substances 0.000 claims abstract description 14
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims description 33
- 125000001931 aliphatic group Chemical group 0.000 claims description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 8
- 241001501873 Isochrysis galbana Species 0.000 claims description 8
- ZPVGIKNDGJGLCO-VGAMQAOUSA-N [(2s,3r,4s,5s,6r)-2-[(2s,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] hexadecanoate Chemical compound CCCCCCCCCCCCCCCC(=O)O[C@@]1([C@]2(CO)[C@H]([C@H](O)[C@@H](CO)O2)O)O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZPVGIKNDGJGLCO-VGAMQAOUSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 230000003014 reinforcing effect Effects 0.000 claims description 5
- 229940074404 sodium succinate Drugs 0.000 claims description 5
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 claims description 5
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 4
- 235000019733 Fish meal Nutrition 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 4
- 241000206607 Porphyra umbilicalis Species 0.000 claims description 4
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 4
- 239000004467 fishmeal Substances 0.000 claims description 4
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 claims description 4
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 claims description 4
- 235000020664 gamma-linolenic acid Nutrition 0.000 claims description 4
- 229960002733 gamolenic acid Drugs 0.000 claims description 4
- 229960001471 sodium selenite Drugs 0.000 claims description 4
- 235000015921 sodium selenite Nutrition 0.000 claims description 4
- 239000011781 sodium selenite Substances 0.000 claims description 4
- 235000013311 vegetables Nutrition 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 7
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 210000004681 ovum Anatomy 0.000 description 6
- 241000512259 Ascophyllum nodosum Species 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 241000238582 Artemia Species 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000009304 pastoral farming Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 241000238366 Cephalopoda Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000133262 Nauplius Species 0.000 description 1
- 206010036590 Premature baby Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention provides screening technique and its application of a kind of tiger spot cuttlefish open-mouthed bait nutrition fortifier, comprises the following steps：1）Tiger spot cuttlefish and open-mouthed bait artemia nauplii content of fatty acid are determined respectively；2）It is higher to tiger spot cuttlefish content, but the unrighted acid of shortage is identified in open-mouthed bait, draws the unrighted acid that open-mouthed bait is lacked；3）Screening unrighted acid supplementary source, microalgae of the screening rich in lacked unrighted acid；4）It is that hardening agent strengthens artemia nauplii with the microalgae for screening with artemia nauplii as carrier；5）The artemia nauplii bait feeding tiger spot cuttlefish that will strengthen, determines the change of tiger spot cuttlefish content of fatty acid, the screening technique of this tiger spot cuttlefish open-mouthed bait nutrition fortifier that the present invention is provided and its application, greatly improves tiger spot cuttlefish seed early stage survival rate.
Description
Technical field
The present invention relates to mariculture technology, especially a kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier
And its application.
Background technology
Tiger spot cuttlefish is the important ocean siphonopods of China coastal seas, and its fast growth, life cycle is short, but current tiger spot
The propagation in scale technology also prematurity of cuttlefish, tiger spot cuttlefish early stage survival rate is relatively low, and sometimes even less than 10%.
Prior art such as, Pharaoh cuttlefish breeding method, CN103548733A it include parent temporarily support, the collection of ovum, ovum
The step of hatching, larval culture, feed device using clamping-rope type cultivation and feed fresh and alive small fish shrimp, improve the utilization of bait
Rate, and grazing rate is also more excellent compared with prior art, contributes to parent to build up health, and can effectively prevent the water caused by residual bait
Matter is ruined.Pharaoh cuttlefish egg taking device has the attached ovum rate higher compared with prior art, and is easy to transport and shifts, simultaneously as
The processing mode that the Pharaoh cuttlefish egg taking device with ovum string is stood 1-3 minutes in atmosphere is employed, by the water in ovum string list face
The egg membrane surface of tiger spot cuttlefish can be stimulated while draining, more conventional technological means has membrane rate higher.Thus, should
Invention has the advantages that simple operation, parent loss are small, ovum grain membrane rate is high, water pollution is small, be suitable to large-scale production.
The method that tiger spot cuttlefish is cultivated in existing technology is a lot, but raising to tiger spot cuttlefish early stage survival rate does not have
Making gravely improves, and most technology is all used to improve the cultural method of tiger spot cuttlefish growth, does not make improvement from feed and carries
Rise the survival rate of tiger spot cuttlefish.
The content of the invention
The technical problems to be solved by the invention there are provided, and increase the scarce aliphatic acid of tiger spot cuttlefish institute, improve tiger spot crow
A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier of crafty getting up early survival rate and its application.
To solve above-mentioned existing technical problem, the present invention uses following scheme：A kind of tiger spot cuttlefish open-mouthed bait nutrition
The step of screening technique of hardening agent, screening technique is：
1）, respectively tiger spot cuttlefish and open-mouthed bait artemia nauplii content of fatty acid determined；
2）, it is higher to content in tiger spot cuttlefish, but in open-mouthed bait lack unrighted acid identified；
3）, screening unrighted acid supplementary source, screening rich in lacked unrighted acid microalgae simultaneously check；
4）, with microalgae as principal component be made hardening agent raise artemia nauplii；
5）, the artemia nauplii after raising is fed and gives tiger spot cuttlefish, determine tiger spot cuttlefish content of fatty acid.
Preferably, it is 22 that the unrighted acid that open-mouthed bait lacked is identified in step 2:The aliphatic acid of 6n 3.
Preferably, verifying in selected microalgae in step 3 scarce content of fatty acid it is high have Isochrysis galbana powder or
Green bar husband bath.
Preferably, the mode for feeding tiger spot cuttlefish of step 5 is：It is tiger to put into the artemia nauplii abundance of food of reinforcing
The 10%~15% of spot cuttlefish gross weight, daily input number of times is 3~4 times, improve tiger spot cuttlefish scarce aliphatic acid content.
A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier, the composition and its weight portion of the hardening agent
For：70~80 parts of Isochrysis galbana powder, 40~50 parts of green bar husband bath, laver powder 10-50 parts, Kelp Powder 15-50 parts, asparagus
Powder 5-10 parts, expanded bean powder 5-10 parts, albumen powder 1-20 weight portions, fish meal 1-5 parts, 1~2 part of ferrous glycine, sodium selenite 0.5
~1 part, sodium succinate 0.1-0.2 parts, sucrose palmitate 0.5-0.8 parts, gamma-Linolenic acid 0.001-0.003 parts.
Preferably, hardening agent is ground into powder, size is 5~16 μm, and artemia nauplii is ingested to hardening agent
Rate, improves the aliphatic acid in artemia nauplii.
Beneficial effect：The present invention provide this tiger spot cuttlefish open-mouthed bait nutrition fortifier screening technique and its should
With improving the content of the scarce aliphatic acid of tiger spot cuttlefish growth institute, greatly raising tiger spot cuttlefish seed early stage survival rate.
Present invention employs above-mentioned technical proposal provide tiger spot cuttlefish open-mouthed bait nutrition fortifier screening technique and its
Using, the deficiencies in the prior art are compensate for, it is reasonable in design, it is easy to operate.
Specific embodiment
It is described in further detail with reference to embodiments：
Embodiment 1,
The step of a kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier, screening technique is：
1）Tiger spot cuttlefish and open-mouthed bait artemia nauplii content of fatty acid are determined respectively；
2）It is higher to content in tiger spot cuttlefish, but the unrighted acid of shortage is identified in open-mouthed bait；
3）Screening unrighted acid supplementary source, microalgae of the screening rich in lacked unrighted acid is simultaneously checked；
4）Artemia nauplii is raised as principal component is made hardening agent with microalgae；
5）Artemia nauplii after raising is fed and gives tiger spot cuttlefish, determine tiger spot cuttlefish content of fatty acid.
In the prior art of the routine techniques dawn known to those skilled in the art in step 1 ~ 5, narration is not in detail made herein.
Preferably, it is 22 that the unrighted acid that open-mouthed bait lacked is identified in step 2:The aliphatic acid of 6n 3.
Preferably, verifying in selected microalgae in step 3 scarce content of fatty acid it is high have Isochrysis galbana powder or
Green bar husband bath.
Preferably, the mode for feeding tiger spot cuttlefish of step 5 is：It is tiger to put into the artemia nauplii abundance of food of reinforcing
The 10% of spot cuttlefish gross weight, daily input number of times is 3 times, improve tiger spot cuttlefish scarce aliphatic acid content.
A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier, the composition and its preferred weight of the hardening agent
Part it is：80 parts of Isochrysis galbana powder, 40 parts of green bar husband bath, 20 parts of laver powder, 30 parts of Kelp Powder, 5 parts of dragon vegetable powder, expanded soyabean
5 parts of powder, 15 parts of albumen powder, 3 parts of fish meal, 1 part of ferrous glycine, 0.5 part of sodium selenite, 0.1 part of sodium succinate, sucrose palmitate
0.5 part, 0.001 part of gamma-Linolenic acid.
Preferably, hardening agent is ground into powder, size is 7 μm, improves the uptake ratio of tiger spot cuttlefish, promotes tiger spot crow
The growth of thief.
Embodiment 2：
A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application, using lower step：1）Using existing skill
Content of fatty acid analyzer in art is determined to tiger spot cuttlefish and open-mouthed bait artemia nauplii content of fatty acid respectively.2）Point
Other that two kinds of aliphatic acid are carried out with investigation and comparison, contrast show that a kind of tiger spot cuttlefish content is higher, but lacks not in open-mouthed bait
Saturated fatty acid, it is then higher to tiger spot cuttlefish content, but the unrighted acid lacked in open-mouthed bait uses gas-chromatography
Identified, the unrighted acid for finally obtaining shortage is 22:The aliphatic acid of 6n 3.3）By books document, network retrieval sieve
Unrighted acid supplementary source is selected, then screening is micro- rich in lacked unrighted acid from various Query Result plants
Algae, the various algae to being found pass through content of fatty acid detector content detection, and final choice is a kind of to contain 22:The aliphatic acid of 6n 3
Value Isochrysis galbana higher, then chooses a kind of containing 22 again:The aliphatic acid of 6n 3 omits the green bar husband bath at bottom.4）With what is screened
Microalgae cooks hardening agent, and the content composition of hardening agent is 70 parts of Isochrysis galbana powder, 50 parts of green bar husband bath, 30 parts of laver powder, sea
30 parts of powder of band, 7 parts of dragon vegetable powder, 8 parts of expanded bean powder, 15 parts of albumen powder, 3 parts of fish meal, 1 part of ferrous glycine, sodium selenite 0.5
Part, 0.2 part of sodium succinate, 0.5 part of sucrose palmitate, 0.001 part of gamma-Linolenic acid, with artemia nauplii as carrier, are used
Hardening agent raises artemia nauplii, and sodium succinate, sucrose palmitate can have with other compositions such as Kelp Powder, dragon vegetable powder etc.
What is imitated gives tiger spot squid larva by carrier by nutrition transmission.Hardening agent is ground into powder particle, and size is 10 μm, artemia without
The section young has grazing rate higher to the particle of 5-16 μm of size, and 22 are rich in being put into two in hardening agent:The algae of the aliphatic acid of 6n 3
Class, one kind based on one kind supplemented by, strengthen hardening agent function.5）The artemia nauplii bait feeding tiger spot cuttlefish that will strengthen,
The artemia nauplii of reinforcing is put into, the worm nauplius amount of input is the 15% of tiger spot cuttlefish gross weight, daily input number of times
It is 4 times, increases the intake of tiger spot cuttlefish, tiger spot cuttlefish is once determined after often cultivating 5 days, records tiger spot cuttlefish fat
Fat acid content changes, when tiger spot cuttlefish 22:The feed can largely be put into the cultivation of tiger spot cuttlefish by the content of fatty acid of 6n 3 when improving
In method, tiger spot cuttlefish early stage survival rate is greatly lifted.
Specific embodiment described herein is only to explanation for example of the invention.The technical field of the invention
Technical staff can be made various modifications or supplement to described specific embodiment or be substituted using similar mode, but
Without departing from of the invention or surmount scope defined in appended claims.
Claims (7)
1. the step of a kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier, screening technique is：
1）, respectively tiger spot cuttlefish and open-mouthed bait artemia nauplii content of fatty acid determined；
2）, it is higher to content in tiger spot cuttlefish, but in open-mouthed bait lack unrighted acid identified；
3）, screening unrighted acid supplementary source, screening rich in lacked unrighted acid microalgae simultaneously check；
4）, with microalgae as principal component be made hardening agent raise artemia nauplii；
5）, the artemia nauplii after raising is fed and gives tiger spot cuttlefish.
2. the screening technique of a kind of tiger spot cuttlefish open-mouthed bait nutrition fortifier according to claim 1, it is characterised in that：
It is 22 that the unrighted acid that open-mouthed bait lacked is identified in the step 2:The aliphatic acid of 6n 3.
3. the screening technique of a kind of tiger spot cuttlefish open-mouthed bait nutrition fortifier according to claim 1, it is characterised in that：
In the step 3 verify in selected microalgae scarce content of fatty acid it is high have Isochrysis galbana powder or green bar husband's bath.
4. the screening technique of a kind of tiger spot cuttlefish open-mouthed bait nutrition fortifier according to claim 1, it is characterised in that：
The mode for feeding tiger spot cuttlefish of the step 5 is：The artemia nauplii abundance of food for putting into reinforcing is tiger spot cuttlefish gross weight
10%~15%, daily input number of times is 3~4 times.
5. a kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier, including hardening agent, it is characterised in that the reinforcing
The composition and its weight portion of agent be：70~80 parts of Isochrysis galbana powder, 40~50 parts of green bar husband bath, laver powder 10-50 parts, sea
15-50 parts of powder of band, 5-10 parts of dragon vegetable powder, expanded bean powder 5-10 parts, albumen powder 1-20 parts, fish meal 1-5 parts, ferrous glycine 1~2
Part, 0.5~1 part of sodium selenite, sodium succinate 0.1-0.2 parts, sucrose palmitate 0.5-0.8 parts, gamma-Linolenic acid 0.001-
0.003 part.
6. the screening technique of a kind of tiger spot cuttlefish open-mouthed bait nutrition fortifier according to power 5, it is characterised in that described
Hardening agent is ground into powder, and size is 5~16 μm.
7. a kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier is used in breeding feed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
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| US6261590B1 (en) * | 1998-01-21 | 2001-07-17 | University Of Maryland Biotechnology Institute | Methods for the enrichment of live feed with nutrients essential for fish larvae |
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| CN105028275A (en) * | 2015-07-09 | 2015-11-11 | 中国科学院南海海洋研究所 | Method for compensating fatty acid for sea horse fries |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
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