CN104957064A - Method for culturing pharaoh cuttlefish fries - Google Patents

Method for culturing pharaoh cuttlefish fries Download PDF

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Publication number
CN104957064A
CN104957064A CN201510386878.8A CN201510386878A CN104957064A CN 104957064 A CN104957064 A CN 104957064A CN 201510386878 A CN201510386878 A CN 201510386878A CN 104957064 A CN104957064 A CN 104957064A
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water
seedling
frame
cuttlefish
hatching
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CN104957064B (en
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莫新
庞庆功
苏李凤
庞鈜
梁海鸥
庞兴军
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Hepu Qingfeng Agricultural Science And Technology Development Co Ltd
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Hepu Qingfeng Agricultural Science And Technology Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a method for culturing pharaoh cuttlefish fries. The method includes the steps that pharaoh cuttlefish eggs are framed and fixed at the position apart from the bottom of a pool by 20 cm-30 cm to be hatched in water; water temperature, salinity and the PH value are accurately regulated and controlled so that the optimal hatching and fry culturing state can be achieved; the environment most suitable for pharaoh cuttlefish hatching and fry culturing is adopted; a shallow-water hatching scheme is adopted, and a water-changing method of flood tides and falling tides in the natural world is simulated so that water consuming can be efficiently managed; scientific daily management operation regulations in pharaoh cuttlefish fry culturing are set. The production cost can be substantially lowered, the hatching rate and the survival rate of the pharaoh cuttlefish fries can be increased, and seedlings higher in quality can be obtained through culturing.

Description

The seedling-cultivating method of a kind of tiger spot cuttlefish
Technical field
The invention belongs to aquaculture category, is the seedling-cultivating method of a kind of tiger spot cuttlefish.
Background technology
Tiger spot cuttlefish, is commonly called as inkfish, is the seawater fish that economic worth is higher.Because the tiger spot cuttlefish resource in ocean is seriously exhausted, market supply shortage, people are anxious treats that cultivating tiger spot cuttlefish by workman fills up the huge vacancy in market.But, the factors such as tiger spot cuttlefish nursery hatchability is low, seedling is uneven, survival rate is low, quantity of exchanged water is large, cause seedling cost too high, the cuttlefish seedling price of the long 3cm of body is up to 6 yuan/tail, seriously constrain the large-scale promotion cultivation of this famous-brand and high-quality kind, handle the problem demanding prompt solutions such as above-mentioned well, to reduce production cost how practically, improve seedling quality, to promoting that the development of tiger spot cuttlefish industry is significant.
Summary of the invention
The present inventor finds the hatching of natural environment optimum tiger spot sea grape and the growth of the young thereof at the South Sea, the variation in water pressure etc. that the intrinsic tide bulge and fall in tiger spot inkfish spawning position, the hatching illuminance of demand and sea area causes, all the very long evolution of tiger spot cuttlefish, the result of reform of nature environment.According to the life habit of tiger spot cuttlefish, simulate corresponding seedling raising environment, correlate tiger spot cuttlefish and ambush the custom of ingesting, carry out hatching, cultivating, significantly can improve hatchability and the survival rate of seedling.
The present invention designs and develops the innovative approach of a kind of tiger spot cuttlefish nursery, allow tiger spot sea grape hatch by the environment near seabed, and within 1st, have the change of twice similar flood tide ebb, effectively can reduce production cost, nursery hatchability and survival rate can be improved, make the fry that hatches evenly, have vigor.
Technical scheme provided by the invention is:
A seedling-cultivating method for tiger spot cuttlefish, comprising:
Step 1, the selected dark 1.2-1.5m in pond, each cement pit area is about 15m 2, choose frame height 15-20cm, dark, PVC plastic frame dress ovum with cover hatch.
Step 2, first laying oxygen increasing tube at the bottom of hatchery, and be connected to aerator, again according to the length of PVC plastic frame, with stainless steel frame or brick, frame of plastic is fixed on 20-30cm place at the bottom of distance pond, then gauge on cement pool wall subscript, is 0 with frame of plastic top horizontal plane, on frame top, 50cm place is+50, then on oxygen increasing tube, under the horizontal line of frame of plastic top, the electrical bar of hanging intelligence, every 5 square metres of electrical bars hanging 1 2kW.
Step 3, when getting ovum, to cut off the adherance such as marine alga, palm rope, do not damage as far as possible and twine ovum root, one-tenth ovum string or ovum grain are put into PVC plastic frame, about puts 30 for every square decimeter, close the lid and fasten.
Step 4, hatching water quality requirement pH value 7.4 ± 0.2, salinity 27.5-28.5 ‰, water temperature 25-26 DEG C, water dissolved oxygen > 7.5 mg/litre, ammonia-nitrogen content < 0.1mg/L, content of nitrite < 0.05mg/L.Water inlet is before nursery pond, and first measure indices, what ammonia nitrogen, nitrite were not up to standard will change water, and water dissolved oxygen is solved by oxygenation, and pH value can be heightened with quicklime, turns down with acetic acid or lactic acid bacteria microbe formulation; Salinity can be heightened with bittern, turns down with fresh water; The electrical bar of available intelligence is heated to water body, the plastic bag ice block cooling of available sealing, and water for larval nursing can will use after active carbon purifying, and water inlet was flooded frame of plastic top 0-50cm and hatched.
Step 5, employing shallow water hatching scheme, to reduce costs.Flooding with Chi Shuigang the frame of plastic top that fish-egg is housed is 0, and on frame top, 50cm place is+50, and 6h of first at the uniform velocity intaking every day, makes water level rise to+50 from 0, then at the uniform velocity draining 6h, makes water level be down to 0 from+50; At the uniform velocity to intake again 6h, make water level rise to+50 again, and then at the uniform velocity draining 6h, make water level return back to again 0, go round and begin again, allow fish-egg experience the variation in water pressure of similar tidal bulge lakie, and occur twice high tide level, twice low tide in 1d.
Step 6, hatching and nurturing period, continuous oxygenation, illumination < 150lux, noise < 65db between seedling culturing vehicle.
Step 7, check the developmental state of fish-egg every day, remove contamination at the bottom of 3d is to pond suction 1 time; Start at from Gunther parent fish spawning, hatch to 17d, start frame lid, depauperation or dead egg white are gone out nursery pond, ruins to prevent nursery water quality.
Step 8, hatching through 20d, a large amount of cuttlefish seedlings broken tire and going out successively, single body weight is about 0.2g, according to about 400/m 2density plan next step cultivation.Point pond way is after the fry amount of having hatched in this hatchery of range estimation reaches corresponding cultivation density, and connect water hose frame with the basin slightly larger than frame of plastic and the fish-egg of not hatching is moved to another hatchery continue hatching, point pond, one-level level ground like this, until hatch complete.
Step 9, squid larva nurturing period, to throw something and feed for twice daily in morning and evening bait, with the shrimp seedling isometric with squid larva trunk width or halogen worm live body, as the bait of squid larva, each daily ration, feeding quantity will make the living body bait of water body reach 2-3/L, is equivalent to the 4-5% that every daily ration of feeding is squid larva body weight.
During step 10, larval culture, water quality requirement can relax, water temperature 19-28 DEG C, salinity 23-30 ‰, pH value 6.8-7.8; Remove contamination at the bottom of the suction of pond and change 1 time every other day into, it is identical with between the incubation period that other change water way, environmental requirement etc.Through the cultivation of three weeks, cuttlefish seedling trunk is long just can reach more than 2cm, can sell as commercial seedling.
Preferably, in the seedling-cultivating method of described tiger spot cuttlefish, in described step 2, sea grape is placed in the water of 20-30cm at the bottom of distance pond and hatches.
Preferably, in the seedling-cultivating method of described tiger spot cuttlefish, in described step 5, adopt shallow water hatching scheme, and in natural imitation circle tidal bulge lakie change water way.
Preferably, in the seedling-cultivating method of described tiger spot cuttlefish, in described step 9, with about isometric with squid larva trunk width shrimp seedling or halogen worm live body, as the open-mouthed bait of squid larva, measure each daily ration, feeding quantity with density 2-3/L of living body bait in water body.
Preferably, in the seedling-cultivating method of described tiger spot cuttlefish, in described step 4, use quicklime heighten or turn down pH value with acetic acid, lactic acid bacteria microbe formulation, make it to reach 7.4 ± 0.2; Use bittern heighten or turn down salinity with fresh water, make it to reach 27.5-28.5 ‰; Use the electrical bar of intelligence to heat or with the plastic bag ice block cooling sealed, make water temperature reach 25-26 DEG C.
Preferably, in the seedling-cultivating method of described tiger spot cuttlefish, in described step 6, using workshop illumination < 150 lux as seedling raising environment.
Preferably, sooner or later throw something and feed for twice in described step 9 time of bait is respectively 05:00,19:00.
The present invention devises the seedling-cultivating method of a kind of tiger spot cuttlefish.The first, the present invention adopts and sea grape is placed on 20-30cm place at the bottom of distance pond, and in simulating nature circle, the environment of tiger spot inkfish spawning is hatched; The second, the present invention adopt shallow water hatch scheme, and in natural imitation circle tidal bulge lakie change water way; Three, the present invention adopts about isometric with squid larva trunk width shrimp seedling alives or halogen worm live body, as the open-mouthed bait of squid larva, with density 2-3/L of living body bait in water body as each daily ration, feeding quantity; Four, the present invention adopts quicklime to heighten or turns down pH value with acetic acid, lactic acid bacteria microbe formulation, uses bittern heighten or turn down salinity with fresh water, uses the electrical bar of intelligence to heat or with the plastic bag ice block cooling sealed, regulates and controls nursery water quality; Five, the present invention adopts workshop illumination < 150lux as seedling raising environment.
Accompanying drawing explanation
Fig. 1 is nursery flow chart
Fig. 2 is nursery pond artwork
Fig. 3 is cuttlefish seedling hatching sketch
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to specification word to make those skilled in the art.
As shown in the figure, the invention provides the embodiment of a kind of tiger spot cuttlefish nursery, comprising:
Step 1, the dark 1.2-1.5 rice in selected pond, each cement pit area is about 15m 2, cement pit is preferably half buried, and namely half pond body is embedded in underground, such available buffer temperature sudden change; Choose frame height 15-20cm, dark, PVC plastic frame dress ovum with cover hatches, the length and width size of frame of plastic, so that be operating as suitable.
Step 2, first laying oxygen increasing tube at the bottom of nursery pond, and be connected to aerator, again according to the length of PVC plastic frame, with stainless steel frame or brick, frame of plastic is fixed on 20-30cm place at the bottom of distance pond, the ornaments width of stainless steel frame or brick is slightly less than the length of PVC plastic frame is advisable, then gauge on cement pool wall subscript, with frame of plastic top horizontal plane for 0, on frame top, 50cm place is+50, then on oxygen increasing tube, under the horizontal line of frame of plastic top, the electrical bar of hanging intelligence, at every 5m 2hang the electrical bar of 1 2kW, electrical bar hanging position is very important, because hang on oxygen increasing tube by electrical bar, can make the quick even heat of water temperature, by electrical bar hanging under the horizontal line of frame of plastic top, makes the electrical bar moment remain in water to be unlikely and burn out.
Such as: the cement pit of 4m × 4m × 1.2m, the frame of plastic length of choosing are 50cm × 40cm, oxygen increasing tube is laid on central authorities at the bottom of pond, oxygen increasing tube the right and left puts stainless steel frame or brick, 3 row can be put in every limit, often row can put 6-8 frame of plastic, the width of stainless steel frame should be made 30-40cm or brick is put into 30-40cm wide, is disposed across by frame of plastic above stainless steel frame or brick, and fixed with cord system.
Step 3, when getting ovum, to cut off the adherance such as marine alga, palm rope, do not damage as far as possible and twine ovum root, one-tenth ovum string or ovum grain are put into PVC plastic frame gently, about puts 30 for every square decimeter, close the lid and fasten, drift out to prevent ovum string or ovum grain.
Step 4, hatching water quality requirement pH value 7.4 ± 0.2, salinity 27.5-28.5 ‰, water temperature 25-26 DEG C, water dissolved oxygen > 7.5 mg/litre, ammonia-nitrogen content < 0.1mg/L, content of nitrite < 0.05mg/L.Water inlet, before nursery pond, not only through active carbon purifying process, and first will measure indices, what ammonia nitrogen, nitrite were not up to standard will change water, water dissolved oxygen solves by oxygenation, and pH value can be heightened with quicklime, turns down with acetic acid or lactic acid bacteria microbe formulation; Salinity can be heightened with bittern, turns down with fresh water; The electrical bar of water temperature intelligence is heated, the plastic bag ice block cooling of available sealing, and water inlet was flooded frame of plastic top 0-50cm and hatched.
Such as: heat with the electrical bar of intelligence, water temperature was set between the incubation period and adds electrified regulation lower than 25 DEG C, higher than 25.5 DEG C of power-off; Water temperature is then set lower than 19 DEG C of electrified regulation, higher than 23 DEG C of power-off during larval culture.
Step 5, employing shallow water hatching scheme, to reduce costs.Flooding with Chi Shuigang the frame of plastic top that fish-egg is housed is 0, and on frame top, 50cm place is+50, and 6h of first at the uniform velocity intaking every day, makes water level rise to+50 from 0, then at the uniform velocity draining 6h, makes water level be down to 0 from+50; At the uniform velocity to intake again 6h, make water level rise to+50 again, and then at the uniform velocity draining 6h, make water level return back to again 0, go round and begin again, allow fish-egg experience the variation in water pressure of similar tidal bulge lakie, and occur twice high tide level, twice low tide in 1d.The nursery of water intaking difficulty, can collect the water that nursery is discharged, after disinfectant sterilization, exposure, aeration, precipitation, the filtrations such as chlorine dioxide, recycle.
Step 6, hatching and nurturing period, continuous oxygenation, illumination < 150lux between seedling culturing vehicle, noise < 65db, because cuttlefish seedling is easily frightened tell ink, so do not cause high pitch during operation, try not with strong illumination cuttlefish seedling.
Step 7, check the developmental state of fish-egg every day, find that fish-egg is too crowded, a point frame can be considered, to reduce mutual extruding; Remove contamination at the bottom of 3d is to pond suction 1 time; Start at from Gunther parent fish spawning, hatch to 17d, start frame lid, depauperation or dead egg white are gone out nursery pond, ruins to prevent nursery water quality.Cleaning work will be carried out in water, gently, will not stave fish-egg.
Step 8, hatching through 20d, a large amount of cuttlefish seedlings broken tire and going out successively, single body weight is about 0.2g, according to about 400/m 2density plan next step cultivation.Point pond way is after the fry amount by having hatched in this hatchery of range estimation reaches corresponding cultivation density, untie for being fixed on frame of plastic on stainless steel frame or fragment of brick, connect water hose frame with the basin slightly larger than frame of plastic and the fish-egg of not hatching is moved to the continuation hatching of another hatchery, point pond, one-level level ground like this, until hatch complete.
Step 9, squid larva nurturing period, (05:00,19:00) throws something and feeds for twice bait daily in morning and evening, with the shrimp seedling isometric with squid larva trunk width or halogen worm live body, as the bait of squid larva, each daily ration, feeding quantity will make the living body bait of water body reach 2-3/L, according to shrimp seedling or this density calculation of halogen worm, be equivalent to the 4-5% that every daily ration of feeding is body weight.This bait throwing in design is the habit considering that the ambuscade of cuttlefish is ingested, and generally cuttlefish waits for that prey enters firing area, initiates suddenly to attack, and its firing area is 2-3 times from health length.
During step 10, larval culture, squid larva adaptive capacity strengthens, and water quality requirement can suitably relax, water temperature 19-28 DEG C, salinity 23-30 ‰, pH value 6.8-7.8; Remove contamination at the bottom of the suction of pond and change 1 time every other day into, it is identical with between the incubation period that other change water way, environmental requirement etc.Through the cultivation of three weeks, cuttlefish seedling trunk is long just can reach more than 2cm, can sell as commercial seedling.

Claims (7)

1. a seedling-cultivating method for tiger spot cuttlefish, is characterized in that, comprising:
Step 1, the selected dark 1.2-1.5m in pond, each cement pit area is about 15m 2, choose frame height 15-20cm, dark, PVC plastic frame dress ovum with cover hatch;
Step 2, first laying oxygen increasing tube at the bottom of nursery pond, and be connected to aerator, again according to the length of PVC plastic frame, with stainless steel frame or brick, frame of plastic is fixed on 20-30cm place at the bottom of distance pond, then gauge on cement pool wall subscript, is 0 with frame of plastic top horizontal plane, on frame top, 50cm place is+50, then on oxygen increasing tube, under the horizontal line of frame of plastic top, hanging intelligence electrical bar, every 5m 2hang the electrical bar of 1 2kW;
Step 3, one-tenth ovum string or ovum grain are put into PVC plastic frame, about put 30 for every square decimeter, close the lid and fasten;
Step 4, hatching water quality requirement pH value 7.4 ± 0.2, salinity 27.5-28.5 ‰, water temperature 25-26 DEG C, water dissolved oxygen > 7.5 mg/litre, ammonia-nitrogen content < 0.1mg/L, content of nitrite < 0.05mg/L; Water inlet is to nursery pond pre-test water quality, and what ammonia nitrogen, nitrite were not up to standard will change water, and pH value can regulate with quicklime or acetic acid, lactic acid bacteria microbe formulation; Salinity can regulate with bittern or fresh water; Water temperature can heat with electrical bar or with sealing plastic bag ice block cooling, water for larval nursing can will use after active carbon purifying;
Step 5, water inlet hatching, flooding with Chi Shuigang the frame of plastic top that fish-egg is housed is 0, and on frame top, 50cm place is+50, and every day, elder generation at the uniform velocity intake 6h, made water level rise to+50 from 0, then at the uniform velocity draining 6h, makes water level be down to 0 from+50; At the uniform velocity to intake again 6h, make water level rise to+50 again, and then at the uniform velocity draining 6h, make water level return back to again 0, go round and begin again, allow fish-egg experience the variation in water pressure of similar sea tidal bulge lakie;
Step 6, hatching and cultivating process, continuous oxygenation, between seedling culturing vehicle, illuminance answers < 150lux, noise < 65db;
Step 7, check the developmental state of fish-egg every day, every 3d, inhaled to nursery pond the end and remove contamination 1 time; Start at from Gunther parent fish spawning, hatch to 17d, start frame lid, depauperation or dead egg white are gone out nursery pond, ruins to prevent nursery water quality;
Step 8, hatching through 20d, a large amount of cuttlefish seedlings broken tire and going out, according to about 400/m successively 2density plan next step cultivation; Point pond way of seedling is after the fry amount of having hatched in this nursery pond of range estimation reaches corresponding cultivation density, connects water hose frame the fish-egg of not hatching is moved to another nursery pond continuation hatching with the basin slightly larger than frame of plastic;
Step 9, squid larva nurturing period, bait of throwing something and feeding for twice daily in morning and evening, with the shrimp seedling isometric with squid larva trunk width or halogen worm live body, as the bait of squid larva, each daily ration, feeding quantity will make the living body bait of water body reach 2-3/L;
During step 10, larval culture, water quality requirement can relax, water temperature 19-28 DEG C, salinity 23-30 ‰, pH value 6.8-7.8; Remove contamination at the bottom of the suction of pond and change 1 time every other day into, it is identical with between the incubation period that other change water way, environmental requirement etc.; Through the cultivation of three weeks, cuttlefish seedling trunk is long just can reach more than 2cm, can sell as commercial seedling.
2. the seedling-cultivating method of tiger spot cuttlefish as claimed in claim 1, is characterized in that, in described step 2, ovum string or ovum grain is fixed in the water of 20-30cm at the bottom of distance pond and hatches.
3. the seedling-cultivating method of tiger spot cuttlefish as claimed in claim 1, is characterized in that, the pH value of described step 4 quicklime or acetic acid, lactic acid bacteria microbe formulation regulation and control water for larval nursing, makes it to reach 7.4 ± 0.2; By bittern or fresh water regulation and control salinity, make it to reach 27.5-28.5 ‰.
4. the seedling-cultivating method of tiger spot cuttlefish as claimed in claim 1, is characterized in that, in described step 5, adopts shallow water hatching scheme, and in natural imitation circle tidal bulge lakie change water way, 6h is at the uniform velocity intake or draining continuously again and again.
5. the seedling-cultivating method of tiger spot cuttlefish as claimed in claim 1, is characterized in that, described step 6 workshop illuminance adopts < 150lux as hatching seedling raising environment.
6. the seedling-cultivating method of tiger spot cuttlefish as claimed in claim 1, it is characterized in that, in described step 9, with about isometric with squid larva trunk width shrimp seedling or halogen worm live body, as the open-mouthed bait of squid larva, and measure each daily ration, feeding quantity with density 2-3/L of living body bait in water body.
7. the seedling-cultivating method of tiger spot cuttlefish according to claim 1, is characterized in that, the time of bait of sooner or later throwing something and feeding for twice in described step 9 is respectively 05:00,19:00.
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CN106841519A (en) * 2016-12-30 2017-06-13 浙江海洋大学 A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application
CN109874704A (en) * 2019-03-29 2019-06-14 宁波大学 A kind of method of tiger spot cuttlefish seed bait domestication
CN109864019A (en) * 2019-04-19 2019-06-11 中国水产科学研究院黄海水产研究所 Lai Shi intends cuttlefish incubating oosperm system and application method
CN109864019B (en) * 2019-04-19 2022-04-15 中国水产科学研究院黄海水产研究所 Hatching system for fertilized eggs of sepia reiliana and using method
CN110402851A (en) * 2019-08-28 2019-11-05 浙江海洋大学 The method for culturing seedlings of tiger spot cuttlefish
CN110402851B (en) * 2019-08-28 2022-01-11 浙江海洋大学 Method for breeding cuttlefish with tiger spot

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