CN102939924A - Method for artificially breeding sepia lycidas gray - Google Patents

Method for artificially breeding sepia lycidas gray Download PDF

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Publication number
CN102939924A
CN102939924A CN2012104520538A CN201210452053A CN102939924A CN 102939924 A CN102939924 A CN 102939924A CN 2012104520538 A CN2012104520538 A CN 2012104520538A CN 201210452053 A CN201210452053 A CN 201210452053A CN 102939924 A CN102939924 A CN 102939924A
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seedling
pond
intending
salinity
cuttlefish
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CN102939924B (en
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蒋霞敏
唐锋
彭瑞冰
罗江
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Ningbo University
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Ningbo University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention discloses a method for artificially breeding sepia lycidas gray, and the method is characterized by comprising the following steps of (1) parent breeding: selecting a harmless parent with complete body into a temporary culture cement tank to be temporarily cultivated, feeding the parent with chilled fishes twice a day, wherein the feeding quantity is 2 to 5 percent of the weight of the sepia lycidas gray; (2) artificial breeding: placing an egg attaching device inside the temporary culture cement tank, mating the male and the female parents to lay eggs, and then transferring the eggs into a hatching tank to be hatched; and (3) offspring breeding: transferring the hatched seedlings into a seedling tank, feeding fairy shrimp nauplius or copepoda in the early stage, feeding copepoda, opossum shrimp or larval prawn in the middle stage, and feeding fresh live fishes and small shrimps in the late stage, completing the breeding of the seedling when the length of the seedling is more than 2cm, the trunk length is more than 1.2cm, the trunk width is more than 0.9cm and the weight is more than 0.3g, and taking out the seedlings with water. The method has the advantages of high survival rate, easiness in cultivation, large cultivation specification, strong disease resistance and fast growth.

Description

A kind of method of intending order cuttlefish artificial breeding
Technical field
The present invention relates to a kind of aquatic product animal raise seedling method, especially relate to a kind of method of intending order cuttlefish artificial breeding.
Background technology
Plan order cuttlefish ( Sepia lycidas) be subordinate to Cephalopoda (Cephalopoda), Sepiida (Sepioidea), Sepiidae (Sepiidae ), Sepia ( Sepia), be the kind of dwelling at the stronger end of shallow sea water warm, perch in the offshore waters of continental shelf district 15~100m depth of water, be distributed in the Indian Ocean-Western Pacific.At South China Sea, spawning season is February in October to next year, and this period, the depth of water 60~100m place resource was than horn of plenty; Carry out migration to offshore 15~30m depth of water place 3~May.Ovum invests on the materials such as gorgonian, sargassum, bamboo branch or cord more.Intend large (the long 40cm of maximum trunk of order cuttlefish build, body weight 5kg), breeding cycle is short, growth rapidly, delicious meat, protein content is high, and the comprehensive utilization scope is wide, therefore be a kind of breed variety that has the cultivation prospect, it also is the important fishery kind in China's South Sea economic worth higher improved seeds, tw Taiwan simultaneously.
Since 2003, University Of Ningbo has done more research at aspects such as the reproductive biology of Sepiella maindroni, aquaculture technology.But aspect propagating artificially, found the Sepiella maindroni sex premature, bad phenomenon such as individual little (50g/ are only), fanning economics is not high, has seriously hindered the development of cuttlefish aquaculture.Therefore, select individuality larger, the order cuttlefish is intended in growth faster, is the effective way that solves this bottleneck.Few to the research of intending the order cuttlefish both at home and abroad at present, mainly concentrate on reproductive behavior, epidermis collagen, the young to CO 2Tolerance, fertilization behavior, predation etc., there is not yet up to now the correlative study report of intending order cuttlefish artificial breeding.
Summary of the invention
It is high that technical problem to be solved by this invention provides a kind of survival rate, is easy to cultivation, and forming specification is large, and premunition is strong, the method for the plan order cuttlefish artificial breeding of growing fast.
The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of method of intending order cuttlefish artificial breeding may further comprise the steps:
(1) parents culture: select the bodily form complete, send into temporary curing cement pond without the plan order cuttlefish parent of injury and support temporarily, and the chilled small fish of 10~15cm size of throwing something and feeding, day bait throwing in 2 times, feeding volume is for intending 2%~5% of order cuttlefish weight; Requiring that wherein cement pit is supported temporarily is as follows,
A. condition of culture: parent adopts airing form to cultivate, and periodic replacement is through the seawater of precipitation, sand filtration, and a day quantity of exchanged water is 100%, removes every day such as residual bait etc., keeps cultivating 14~25 ℃ of water temperatures, salinity 28~30, and dissolved oxygen is 10~14.50mg/ L, pH 7.81~8.04;
B. breeding density: male and female are raised together with, and female-male proportion is 2:1~3:1, and breeding density is with 1~3/m 2Be advisable;
(2) artificial propagation
A. lay eggs: place egg apparatus in described temporary curing cement pond, after the male and female parent carried out mating, internal fertilization, female plan order cuttlefish laid eggs to egg apparatus, and the water temperature of laying eggs is 16.4~25.0 ℃, and salinity is 28~30;
B. hatching: the fertilized egg that will obtain after will laying eggs is transferred in the hatchery, in the plastics screen frame of hatchery and bolting silk screen frame, hatch, incubation temperature is 21~27 ℃, salinity is 25~30, brooding time 21d~35d, Spawning water is successively through sand filtration, obscure shallow lake and acrylic staple fibre bag filtration, and the individual layer sunshade net is added a cover in the top, hatchery;
(3) seed rearing: the seedling of incubation of membrane is moved on in the seedling pond, throw something and feed early stage through 3~5mm fairy shrimp nauplius of single-cell algae reinforcement or the copepoda of 1.5~5mm, food density is 1~2/mL; Throw something and feed the mid-term copepoda of 1.5~13mm, oppossum shrimp or the 0.5~1.0cm larvae of Penaeus vannamei of 5~25mm, food density is 5~10/L; Throw something and feed fresh and alive small fish and 2.0~5.0cm small shrimps of later stage, when the seedling total length greater than 2cm, trunk is grown up in 1.2cm, trunk is wider than 0.9cm, body weight is during greater than 0.3g, seedling culture is finished, band water is emerged, wherein the seedling pool environment requires as follows:
A. nurturing an environment: it is 21~27 ℃ that water temperature is cultivated in described seedling pond, and salinity is that 23~30, pH is 7.81~8.04, and the depth of water 1.0~1.5 is advisable, per 1~2m 2Place a gas exhausting stone, every day, quantity of exchanged water was 50~60%, and in time removed dead individuality and residue dirt;
B. young density: seedling is with 1000/m in the described seedling pond 3~1500/m 3Be advisable.
The middle parent of step (1) is sent into the transportation lorry that adopts in the process in temporary curing cement pond 2.0 * 1.5. * 1.0m is housed 3Nylon canvas, the ocean temperature that fills in the described transportation lorry is 14~25 ℃, salinity is 28~30, and is filled with oxygen.
Temporary curing cement pond size described in the step (1) is 5.0 * 4.3 * 1.0m 3, described temporary curing cement evenly is provided with 8~12 inflating air stones in the pond.
In described temporary curing cement pond, splash in the step (1) povidone iodine of 10~20mg/L.Because cuttlefish has slight damage in handling process, be used for it is carried out disinfection anticorrosion.
Spawning pond top described in the step (2) evenly is provided with the some stationary ropes that are used for hanging described egg apparatus, described stationary rope spacing each other is 70-90cm, described egg apparatus upper end is fixed on the described stationary rope, there is the sinker of heavy 0.1-0.2kg in its lower end system, and leaves the space of 4-5cm at the bottom of the pond of described egg apparatus and described spawning pond.
The described egg apparatus of step (2) is that mesh is the polythene net sheet of 25-30mm, described polythene net sheet evenly be suspended on the described stationary rope and on every stationary rope spacing distance be 0.8-1m, described polythene net sheet is of a size of 30cm * 40cm.Because the surface area of net sheet is larger, in embryo development procedure, is not easy pinch between the fertilized egg, and simple for production, can throw on a large scale.
The described egg apparatus of step (2) is that polythene net sheet and the diameter of mesh 25-30mm is the nylon rope of 8mm-12mm, unified described polythene net sheet or the described nylon rope of hanging on the every described stationary rope, the stationary rope that hangs described polythene net sheet comes with the stationary rope space that hangs described nylon rope, the spacing distance of described polythene net sheet on described stationary rope is 0.8-1m, the spacing distance of described nylon rope on described stationary rope is 0.8-1m, described polythene net sheet is of a size of 30cm * 40cm, the length 1.5m of described nylon rope.Because the surface area of net sheet and the diameter of nylon rope are larger, in embryo development procedure, are not easy pinch between the fertilized egg, and simple for production, can throw on a large scale.
Intending order hatching cuttlefish temperature in the step (2) is 24 ℃, and salinity is 30, and hatching density is controlled at 3~6/L.Under this incubation temperature, salinity and density, can guarantee higher incubation rate, shorter incubation cycle, cultivation period, and guarantee the higher absorption ratio of lower first abnormal rate, yolk sac of incubating the young, the larger first young.
The process that in the step (2) fertilized egg is transported to the hatchery from temporary curing cement pond is: fertilized egg is adopted the transportation resources of double-layer nylon bag oxygenation, and oxygenation bag specification is 75 * 75 * 55cm 3, put into subsequently 48 * 48 * 25cm 3Bubble chamber in, bubble chamber dress ovum 4~5kg is filled with water 1/3, water temperature is 22 ℃ during transportation, salinity is 30.
Step (3) bait with throw something and feed, be the stage of 0.8~1.0cm for the trunk length of intending the order cuttlefish early stage, be the stage of 1.0~2.0cm for the trunk length of intending the order cuttlefish mid-term, the later stage is the stage of the trunk length>2.0cm that intends the order cuttlefish.
Compared with prior art, the invention has the advantages that: this seedling-cultivating method has the advantages such as incubation rate is high, survival rate is high, disease is few, the amount of growing seedlings is high, be easy to cultivation, grow soon, trimmed size is large, and the operation easy to implement of this seedling-cultivating method, success rate are high, suitable large-scale promotion.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
One, specific embodiment
Embodiment 1
A kind of method of intending order cuttlefish artificial breeding of the present invention specifically may further comprise the steps:
1, parents culture
(1) parent source and transportation
Mid-March~late April is with the colony of Zhaoan County, Zhangzhou City, small-sized trawl fishing Fujian Province natural waters egg-laying migration.Select the bodily form complete, put into the device that fills seawater and oxygenation without the parent of injury and support temporarily, and deliver to as early as possible temporary curing cement pond.Adopt during transportation 2.0 * 1.5. * 1.0m is housed 3The buggy of nylon canvas, it is 14~25 that temperature is housed on the car, salinity is 28~30 seawater, and oxygenation.Transportation time spent 0.5h, average transportation survival rate is 95.26%, in case find to have ink-jet individual, should immediately prepared Chinese ink be dragged for or water is changed in full pond;
(2) parent reinforced cultivating
A. breeding condition
The curing cement pond is 5.0 * 4.3 * 1.0m temporarily 3Cement pit, 8~12 inflating air stones evenly are set in the pond, breeding water is nature seawater through precipitation, sand filtration, water quality is limpid, cultivates 14~25 ℃ of water temperatures, salinity 28~30, dissolved oxygen is 14.50mg/ L, pH 7.81~8.04.Because cuttlefish has slight damage in handling process, the povidone iodine of the 10~20mg/L that need in temporary curing cement pond, splash.In case find to have ink-jet individual, should immediately prepared Chinese ink be dragged for or change water;
B. breeding density
Male and female are raised together with, and female ratio is (2:1)~(3:1), and breeding density is with 1/m 2~3/m 2Be advisable;
C. bait with throw something and feed
The chilled small fish of 10~15cm size of throwing something and feeding, day bait throwing in 2 times, feeding volume is 2%~5% of cuttlefish weight;
D. water quality management
Parent adopts airing form to cultivate, the periodic replacement seawater, and a day quantity of exchanged water is 100%.Remove every day such as residual bait etc.Keep 14~25 ℃ of water temperatures, salinity 28~30, dissolved oxygen is>10mg/ L that pH 7.81~8.04.
2, artificial propagation
(1) making of egg apparatus and placement
Above spawning pond, evenly be provided with some stationary ropes, each stationary rope spacing each other is 70-90cm, get mesh and be the polythene net sheet of 25-30mm as egg apparatus, evenly be suspended on the stationary rope and on every stationary rope spacing distance be 0.8-1m, this polythene net sheet is of a size of 30cm * 40cm, the egg apparatus upper end is fixed on the stationary rope by connecting rope, the sinker of heavy 0.1-0.2kg is fastened in its lower end, make it be in stretching state, and make the space of leaving 4-5cm at the bottom of the pond of egg apparatus and spawning pond.The parent front placement egg apparatus of laying eggs.Egg apparatus needs through the potassium permanganate strict sterilization, and fresh seawater is rushed and laid after quiet again.Clean the not egg apparatus of attached ovum 1 time every 2~3d;
(2) male and female are differentiated
General male is large than female individuals, and male body colour is yellowish-brown, and is more bright-coloured, and female is taupe; The male back side " eye mouth " decorative pattern is larger, left side the 4th wrist stem, and the sucker at full wrist middle part suddenly diminishes and is sparse.Male in order to fight for the spouse, can open as possible 4 pairs of wrists (except tactile wrist) and fight, warning colouration appears simultaneously, and it is unusually bright-coloured that body colour becomes.Ripe male dissection is visible larger ink sac, spermary, spermatophore sac and sperm-storing bag afterwards.By-product ovum gland, the ovary of visible a pair of yellow after adult female is dissected;
(3) lay eggs
Intending the order cuttlefish is internal fertilization, and during mating, male and female are tightly embraced together by each wrist, mating complete separately after a few minutes, femalely under the accompanying of hero, lay eggs to egg apparatus.The water temperature of laying eggs is 16.4~25.0 ℃, and salinity is 28~30.From common property ovum on the 23rd 237.5kg in March 16 to April, be about 59375,261.5 of average every inkfish spawnings;
(4) transportation of fertilized egg
Fertilized egg is adopted the transportation resources of double-layer nylon bag oxygenation, and oxygenation bag specification is 75 * 75 * 55cm 3, put into subsequently 48 * 48 * 25cm 3Bubble chamber in.A bubble chamber can fill ovum 4~5kg at most, is filled with water 1/3.Water temperature is 22 ℃ during transportation, and salinity is 30.For fear of serious extruding, affect hatchability of fertile eggs, then need to be attached with the rope of ovum and net sheet and keep flat neatly and transport again.All be careful when packing and transportation and put down gently, to reduce the stimulation to fertilized egg, raise for fear of water temperature in transportation simultaneously, need in each bubble chamber, put into 2 ice chests;
(5) hatching
A. environment of hatching
Fertilized egg is placed in plastics screen frame and the bolting silk screen frame hatches; The hatchery is 4.0 * 8.0 * 1.6m 3, the plastics screen frame is 55 * 55 * 20cm 3, the bolting silk screen frame is 50 * 50 * 100cm 3The hatching salinity is 25~30, and temperature is 21~27 ℃.Spawning water is through sand filtration, obscure shallow lake and acrylic staple fibre bag filtration, and the individual layer sunshade net is added a cover in the top, hatchery;
B. brooding time
In normal water temperature range, cuttlefish embryonic development required time and water temperature just are inversely proportional to, under the condition of 27~30 ℃ of water temperatures, and average brooding time 17~24d; Under 21~27 ℃ of the water temperatures, average brooding time 24d~35d;
C. hatch results
Hatch altogether 23050 of cuttlefishes from April 16 to May 20.Just incubate long (8.1 ± 0.2) mm of cuttlefish trunk, trunk wide (6.1 ± 0.2) mm, body weight (0.18 ± 0.02) g.
3, seed rearing
(1) nurturing an environment: the seedling of incubation of membrane is moved on to the seedling pond, and the seedling pond is 4.0 * 8.0 * 1.6m 3, salinity is 23~30, and temperature is 21~27 ℃, and the depth of water 1.0~1.5m is advisable, per 1~2m 2Place a gas exhausting stone;
(2) Larval quality: behind the young cuttlefish membrane, be active in the pond at the bottom of, can swim in looking for food at the middle and upper levels, energetic being the healthy and strong young night; Otherwise be the young inferior;
(3) daily management
A. bait with throw something and feed
Intending the order cuttlefish is carnivorous siphonopods, the young cuttlefish zooplanktons such as copepoda, cladocera or oppossum shrimp of liking ingesting, the adult live baits such as fish, shrimp of liking ingesting.Specifically as shown in table 1.
Table 1 each phase food species and feeding volume
The seedling of incubation of membrane is moved on in the seedling pond, throw something and feed early stage 3~5mm fairy shrimp nauplius of strengthening through single-cell algae or copepoda, food density is 1~2/mL; Throw something and feed the mid-term copepoda of 1.5~13mm, oppossum shrimp or the 0.5~1.0cm larvae of Penaeus vannamei of 5~25mm, food density is 5~10/L; Throw something and feed fresh and alive small fish and 2.0~5.0cm small shrimps of later stage, when the seedling total length greater than 2cm, trunk is grown up in 1.2cm, trunk is wider than 0.9cm, body weight is during greater than 0.3g, seedling culture is finished, band water is emerged;
B. water quality management
Salinity is 23~30, and temperature is 21~27 ℃, and pH is 7.81~8.04.Under normal circumstances, every day, quantity of exchanged water was 50~60%, and in time removed dead individuality and residue dirt.The sampling siphonage is grasped the principle that slow-motion goes out slowly when changing water, in order to avoid water environment changes the excessive cuttlefish ink-jet that causes.Will change a little fresh seawater between the megathermal period much more as far as possible, illumination will cover sunshade net when too strong.At heavy rain or typhoon weather, notice that salinity changes, the rapid reduction of salinity easily causes cuttlefish uncomfortable even dead, need to slowly add crude salt and regulate, and is no more than 5 ‰ to keep the salinity luffing;
C. inflation
Along with the cuttlefish growth, its oxygen demand also increases, therefore can adopt little inflation the early stage of growing seedlings, the later stage should be strengthened aeration quantity;
(4) emerge, pack and transport
A. specification and quality
Selecting specification is total length 2cm, the long 1.2cm of trunk, the wide 0.9cm of trunk, body weight 0.3g, and size evenly, body surface totally, not damaged and deformity, plan order cuttlefish that vigor is stronger;
B. emerge
First Chi Shui is drained into the depth of water when emerging approximately behind the 30cm, to drum, then be with water to count one by one and put into Nylon Bag with soft diddle-net band water collection seedling;
C. pack
The general transportation resources of adopting the Nylon Bag oxygenation.Specification 35 * 35 * 40cm 3Nylon Bag can put 100 seeds, specification 75 * 75 * 55cm 3Nylon Bag can put 200 seeds, put into subsequently 48 * 48 * 25cm 3Bubble chamber in.Seed transportation should select shady and cool weather and morning and evening to carry out, and water temperature is 24 ℃ when transporting, and salinity is 25;
D. transportation survival rate
Packing all is careful with when transportation and is put down gently, reducing the stimulation to seed, otherwise can cause the seed ink-jet and affects transportation survival rate.Transportation on May 8 cuttlefish seed time spent 12h, survival rate 86%; Transportation on May 23 seed time spent 3h, survival rate 95%.
Embodiment 2
With embodiment 1, its distinguishing characteristics is: 14 ℃ of (1) parents culture water temperatures, and salinity 28, dissolved oxygen is 10mg/ L, pH 7.81; (2) water temperature of laying eggs is 16.4 ℃, and salinity is 28; (3) the hatching salinity is 25, and temperature is 21 ℃, average brooding time 34d; (4) the seed rearing temperature is 21 ℃, and salinity is that 23, pH is 7.81; (5) when the seedling total length be 3cm, trunk is grown up in 1.5cm, trunk is wide to be 1.2cm, when body weight was 0.5g, seedling culture was finished.(6) stationary rope spacing each other is 80cm, the egg apparatus upper end is fixed on the stationary rope, there is the sinker of heavy 0.1-0.2kg in its lower end system, and leave the space of 4-5cm at the bottom of the pond of egg apparatus and spawning pond, above-mentioned egg apparatus is that polythene net sheet and the diameter of mesh 25-30mm is the nylon rope of 8mm-12mm, unified polythene net sheet or the nylon rope of hanging on the every stationary rope, the stationary rope that hangs the polythene net sheet comes with the stationary rope space that hangs nylon rope, the spacing distance of polythene net sheet on stationary rope is 1m, the spacing distance of nylon rope on stationary rope is 1m, the polythene net sheet is of a size of 30cm * 40cm, the length 1.5m of nylon rope; (7) seedling with incubation of membrane moves on in the seedling pond, 3~5mm fairy shrimp nauplius that throw something and feed and strengthen through single-cell algae early stage, and food density is 1/mL; The throw something and feed mid-term copepoda of 1.5~13mm, food density is 5/L; Throw something and feed fresh and alive small fish and 2.0~5.0cm small shrimps of later stage.
Embodiment 3
With embodiment 1, its distinguishing characteristics is: 25 ℃ of (1) parents culture water temperatures, and salinity 30, dissolved oxygen is 12.50mg/ L, pH 8. 04; (2) water temperature of laying eggs is 25.0 ℃, and salinity is 30; (3) the hatching salinity is 30, and temperature is 27 ℃, average brooding time 24d; (4) the seed rearing temperature is 27 ℃, and salinity is that 30, pH is 8.04; (5) as seedling total length 2.5cm, the long 1.4cm of trunk, the wide 1.1cm of trunk, during body weight 0.4g, seedling culture is finished; (6) seedling with incubation of membrane moves on in the seedling pond, the copepoda of the 1.5~5mm that throws something and feeds early stage, and food density is 2/mL; The throw something and feed mid-term oppossum shrimp of 5~25mm, food density is 8/L; Throw something and feed fresh and alive small fish and 2.0~5.0cm small shrimps of later stage.
Embodiment 4
With embodiment 1, its distinguishing characteristics is: 20 ℃ of (1) parents culture water temperatures, and salinity 29, dissolved oxygen is 13.50mg/ L, pH 8; (2) water temperature of laying eggs is 22 ℃, and salinity is 29; (3) the hatching salinity is 28, and temperature is 25 ℃, average brooding time 28d; (4) the seed rearing temperature is 23 ℃, and salinity is that 26, pH is 7.9; (5) when the seedling total length greater than 2cm, the long 1.5cm of trunk, the wide 1.3cm of trunk, during body weight 0.35g, seedling culture is finished; (6) seedling with incubation of membrane moves on in the seedling pond, the copepoda of the 1.5~5mm that throws something and feeds early stage, and food density is 2/mL; 0.5~1.0cm larvae of Penaeus vannamei of throwing something and feeding mid-term, food density is 5~10/L; Throw something and feed fresh and alive small fish and 2.0~5.0cm small shrimps of later stage.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention also is not limited to above-mentioned giving an example.Those skilled in the art are in essential scope of the present invention, and the variation of making, remodeling, interpolation or replacement also should belong to protection scope of the present invention, and protection scope of the present invention is as the criterion with claims.

Claims (10)

1. method of intending order cuttlefish artificial breeding is characterized in that may further comprise the steps:
(1) parents culture: select the bodily form complete, send into temporary curing cement pond without the plan order cuttlefish parent of injury and support temporarily, and the chilled small fish of 10~15cm size of throwing something and feeding, day bait throwing in 2 times, feeding volume is for intending 2%~5% of order cuttlefish weight; Requiring that wherein cement pit is supported temporarily is as follows,
A. condition of culture: parent adopts airing form to cultivate, and periodic replacement is through the seawater of precipitation, sand filtration, and a day quantity of exchanged water is 100%, removes every day such as residual bait etc., keeps cultivating 14~25 ℃ of water temperatures, salinity 28~30, and dissolved oxygen is 10~14.50mg/ L, pH 7.81~8.04;
B. breeding density: male and female are raised together with, and female-male proportion is 2:1~3:1, and breeding density is with 1~3/m 2Be advisable;
(2) artificial propagation
A. lay eggs: place egg apparatus in described temporary curing cement pond, after the male and female parent carried out mating, internal fertilization, female plan order cuttlefish laid eggs to egg apparatus, and the water temperature of laying eggs is 16.4~25.0 ℃, and salinity is 28~30;
B. hatching: the fertilized egg that will obtain after will laying eggs is transferred in the hatchery, in the plastics screen frame of hatchery and bolting silk screen frame, hatch, incubation temperature is 21~27 ℃, salinity is 25~30, brooding time 21d~35d, Spawning water is successively through sand filtration, obscure shallow lake and acrylic staple fibre bag filtration, and the individual layer sunshade net is added a cover in the top, hatchery;
(3) seed rearing: the seedling of incubation of membrane is moved on in the seedling pond, throw something and feed early stage through 3~5mm fairy shrimp nauplius of single-cell algae reinforcement or the copepoda of 1.5~5mm, food density is 1~2/mL; Throw something and feed the mid-term copepoda of 1.5~13mm, oppossum shrimp or the 0.5~1.0cm larvae of Penaeus vannamei of 5~25mm, food density is 5~10/L; Throw something and feed fresh and alive small fish and 2.0~5.0cm small shrimps of later stage, when the seedling total length greater than 2cm, trunk is grown up in 1.2cm, trunk is wider than 0.9cm, body weight is during greater than 0.3g, seedling culture is finished, band water is emerged, wherein the seedling pool environment requires as follows:
A. nurturing an environment: it is 21~27 ℃ that water temperature is cultivated in described seedling pond, and salinity is that 23~30, pH is 7.81~8.04, and the depth of water 1.0~1.5 is advisable, per 1~2m 2Place a gas exhausting stone, every day, quantity of exchanged water was 50~60%, and in time removed dead individuality and residue dirt;
B. young density: seedling is with 1000/m in the described seedling pond 3~1500/m 3Be advisable.
2. a kind of method of intending order cuttlefish artificial breeding according to claim 1 is characterized in that: parent is sent into the transportation lorry that adopts in the process in temporary curing cement pond 2.0 * 1.5. * 1.0m is housed in the step (1) 3Nylon canvas, the ocean temperature that fills in the described transportation lorry is 14~25 ℃, salinity is 28~30, and is filled with oxygen.
3. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that: the temporary curing cement pond size described in the step (1) is 5.0 * 4.3 * 1.0m 3, described temporary curing cement evenly is provided with 8~12 inflating air stones in the pond.
4. it is characterized in that: the povidone iodine of the 10~20mg/L that in described temporary curing cement pond, splashes in the step (1) according to claim 1 or 3 described a kind of methods of intending order cuttlefish artificial breeding.
5. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that: the spawning pond top described in the step (2) evenly is provided with the some stationary ropes that are used for hanging described egg apparatus, described stationary rope spacing each other is 70-90cm, described egg apparatus upper end is fixed on the described stationary rope, there is the sinker of heavy 0.1-0.2kg in its lower end system, and leaves the space of 4-5cm at the bottom of the pond of described egg apparatus and described spawning pond.
6. a kind of method of intending order cuttlefish artificial breeding according to claim 5, it is characterized in that: the described egg apparatus of step (2) is that mesh is the polythene net sheet of 25-30mm, described polythene net sheet evenly be suspended on the described stationary rope and on every stationary rope spacing distance be 0.8-1m, described polythene net sheet is of a size of 30cm * 40cm.
7. a kind of method of intending order cuttlefish artificial breeding according to claim 5, it is characterized in that: the described egg apparatus of step (2) is that polythene net sheet and the diameter of mesh 25-30mm is the nylon rope of 8mm-12mm, unified described polythene net sheet or the described nylon rope of hanging on the every described stationary rope, the stationary rope that hangs described polythene net sheet comes with the stationary rope space that hangs described nylon rope, the spacing distance of described polythene net sheet on described stationary rope is 0.8-1m, the spacing distance of described nylon rope on described stationary rope is 0.8-1m, described polythene net sheet is of a size of 30cm * 40cm, the length 1.5m of described nylon rope.
8. a kind of method of intending order cuttlefish artificial breeding according to claim 1 is characterized in that: intending order hatching cuttlefish temperature in the step (2) is 24 ℃, and salinity is 30, and hatching density is controlled at 3~6/L.
9. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that the process that in the step (2) fertilized egg is transported to the hatchery from temporary curing cement pond is: fertilized egg is adopted the transportation resources of double-layer nylon bag oxygenation, and oxygenation bag specification is 75 * 75 * 55cm 3, put into subsequently 48 * 48 * 25cm 3Bubble chamber in, bubble chamber dress ovum 4~5kg is filled with water 1/3, water temperature is 22 ℃ during transportation, salinity is 30.
10. a kind of method of intending order cuttlefish artificial breeding according to claim 1, it is characterized in that: step (3) bait with throw something and feed, be the stage of 0.8~1.0cm for the trunk length of intending the order cuttlefish early stage, be the stage of 1.0~2.0cm for the trunk length of intending the order cuttlefish mid-term, and the later stage is the stage of the trunk length>2.0cm of plan order cuttlefish.
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CN103548732A (en) * 2013-10-30 2014-02-05 浙江海洋学院 Artificial offspring seed cultivating method for pharaoh cuttlefish
CN103548733A (en) * 2013-10-30 2014-02-05 浙江海洋学院 Pharaoh cuttlefish breeding method
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CN104957064A (en) * 2015-07-06 2015-10-07 合浦县庆丰农业科技开发有限公司 Method for culturing pharaoh cuttlefish fries
CN105994016A (en) * 2015-12-09 2016-10-12 江苏省海洋资源开发研究院(连云港) Method for harvesting and temporarily culturing sepia esculenta in cuttlefish-shrimp polyculture pond
CN105994016B (en) * 2015-12-09 2020-01-14 江苏省海洋资源开发研究院(连云港) Method for harvesting and temporarily culturing sepia esculenta in cuttlefish and shrimp polyculture pond
CN109362610A (en) * 2018-03-22 2019-02-22 中国水产科学研究院黄海水产研究所 Lai Shi intends cuttlefish offspring seed cultivation method
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CN109430116A (en) * 2018-08-27 2019-03-08 吴常文 A kind of quasi- mesh cuttlefish offspring seed cultivation method
CN109430117A (en) * 2018-08-27 2019-03-08 吴常文 Improve quasi- mesh cuttlefish reproductive capacity method
CN109430118A (en) * 2018-08-27 2019-03-08 吴常文 The method for improving tiger spot cuttlefish reproductive capacity
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CN109287526A (en) * 2018-09-22 2019-02-01 连云港侨海渔业科技有限公司 A kind of golden cuttlefish parent is raised and train and germination method in advance
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