CN103548732A - Artificial offspring seed cultivating method for pharaoh cuttlefish - Google Patents

Artificial offspring seed cultivating method for pharaoh cuttlefish Download PDF

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CN103548732A
CN103548732A CN201310522961.4A CN201310522961A CN103548732A CN 103548732 A CN103548732 A CN 103548732A CN 201310522961 A CN201310522961 A CN 201310522961A CN 103548732 A CN103548732 A CN 103548732A
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cuttlefish
egg
tiger spot
ovum
spot cuttlefish
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CN103548732B (en
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吕振明
常抗美
朱爱意
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Zhejiang Dahaiyang Science and Technology Co., Ltd.
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Zhejiang Ocean University ZJOU
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract

The invention discloses an artificial offspring seed cultivating method for pharaoh cuttlefish. The artificial offspring seed cultivating method comprises steps of parent temporary rearing, egg collection, egg incubation and larva cultivation. A rope holding type cultivation feeding device is utilized to feed the pharaoh cuttlefish with fresh small fishes and shrimps, therefore, the utilization rate of baits is increased, the feeding rate is higher than that of the prior art, physique of parents is invigorated, and worse water quality caused by residual feeds can be effectively avoided. Compared with the prior art, a pharaoh cuttlefish egg collection device is high in egg adhesion rate and easy to transport and transfer. By means of a mode of standing the pharaoh cuttlefish egg collection device adhered by egg strings in air for 1-3 minutes, water on the surface of the egg strings is drained off, stimulation is performed on the surfaces of egg membranes of the pharaoh cuttlefish, and the membrane coming rate is higher than that of the conventional technological means. The incubation rate is further increased by means of incubation reinforcement solution. Therefore, the artificial offspring seed cultivating method has the advantages of being convenient to operate, small in parent loss, high in membrane coming rate of eggs, less in water pollution and suitable for large-scale production.

Description

Tiger spot cuttlefish artificial seed breeding method
Technical field
The present invention relates to a kind of siphonopods seedling-cultivating method, relate in particular to tiger spot cuttlefish artificial seed breeding method.
Background technology
Tiger spot Sepia Sepiida, Sepiidae, Sepia a kind of.Mainly being distributed in China's Mainland, Taiwan, lays eggs in the neritic area of being everlasting, and dwells winter in 100 meters of left and right of the depth of water.Cluster was swum phytal zone mating, was laid eggs spring; In the egg-laying period in coastal waters, Guangdong, be about the 4-5 month, water temperature 18-24 ℃, lays eggs in 5-20 rice depth of water place.Ovum is larger, the about 27-34 millimeter of ovum major diameter before hatching, and the about 14-16 millimeter of minor axis, is slightly botryoidalis, and one end is more prominent, and the other end is a minute fork handle, and egg membrane is cream colour pattern, translucent; Ovum is in batches ripe, single output, and prick invests on Liu Shanshan, sargassum or withe, cord more.Between breeding period, " tiger spot " at the male body back side is distinct especially, and so-called " wedding dress color ", has the idol of chasing after, strives the complicated sexual behavior such as even, and have obvious phototaxis.The growth of tiger spot cuttlefish rapidly, can grow to 4kg in 1 year, is ideal cultivation kind; but artificial breeding technology is not also broken through at present, the main problem existing is not clear to tiger spot cuttlefish early nutrition demand, causes seed rearing early stage; lethality is very high, propagation in scale difficulty.In addition, in the technology of parent feedstuff feeding, larva initial baits, all less than comparatively practical technology, can use.
Summary of the invention
The object of the invention is to for prior art a kind of simple operation is provided, parent loss is little, ovum grain membrane rate is high, emergence rate is high, water pollution is little, be suitable for the tiger spot cuttlefish artificial seed breeding method of large-scale production.
The present invention solves the problems of the technologies described above adopted technical scheme: tiger spot cuttlefish artificial seed breeding method, and it comprises following step:
Figure 2013105229614100002DEST_PATH_IMAGE002
parent is supported temporarily: parent is put into cement pit and support temporarily, parent breeding density is 0.5-2/square metre; Cement pit degree of depth 2-3 rice, the fine sand of paving 10-20 cm thick at the bottom of pond; Temporarily supporting seawater salinity is 28-30 ‰, and water temperature is 24-25 ℃, keeps perennial draingage, shading, and inflation, keeps quite, and adopts the clamping-rope type cultivation device fresh and alive small fish shrimp of throwing something and feeding of throwing something and feeding; The clamping-rope type cultivation device of throwing something and feeding can improve the availability of bait, and grazing rate is also more excellent compared with prior art, contributes to that parent is built up health, loss is less, and can effectively prevent from ruining because of the water quality that residual bait causes.
Figure 2013105229614100002DEST_PATH_IMAGE004
the collection of ovum: by bosom ovum parent by putting into spawning pond, the area 20-25 square metre of spawning pond, depth of water 1.5-3 rice, places tiger spot cuttlefish and adopts egg apparatus in the bottom in pond; Parent breeding density 1-2/square metre, adopt in egg apparatus replacement water body and adopt egg apparatus with the tiger spot cuttlefish of ovum string every day with the tiger spot cuttlefish of cleaning, and the tiger spot cuttlefish with ovum string of collecting is adopted to egg apparatus rest on 1-3 minute in air, then spray hatching reinforced solution, and standing 1-3 minute again; Tiger spot cuttlefish is adopted egg apparatus and has the attached ovum rate higher compared with prior art, and be easy to transportation and shift, simultaneously, owing to having adopted, by adopt egg apparatus with the tiger spot cuttlefish of ovum string, rest in air the processing mode of 1-3 minute, when the water of ovum string list face is drained, can stimulate the egg membrane surface of tiger spot cuttlefish, compared with routine techniques means, have higher membrane rate.
Figure 2013105229614100002DEST_PATH_IMAGE006
the hatching of ovum: the tiger spot cuttlefish with ovum string of same batch is adopted to the concentrated water middle part, hatchery that is suspended on of egg apparatus and hatch, ovum granule density is 300-350/cubic metre; The area 10-15 square metre of hatchery, the dark 1.6-2 rice in pond, every square metre of 1-2 of gas stone, micro-inflation;
Figure 2013105229614100002DEST_PATH_IMAGE008
larval culture: the young hatches the rear oppossum shrimp of directly throwing something and feeding, and illumination is 600-800 lux, micro-inflation, noise keeps, below 70 decibels, when seedling rouge, growing up after 2 centimetres, shifts out seedling outward.
For optimizing technique scheme, the measure of taking also comprises: tiger spot cuttlefish is adopted egg apparatus and has top frame and underframe; Two sides that top frame is relative with underframe have bracing piece; Top frame is also evenly equipped with the parallel flexible bar that adheres to that has in gap with underframe side; Top frame has support bar; Adhere to the nylon plastic(s) that bar is sheet; The width that adheres to bar is 0.5cm to 1.5cm; The thickness that adheres to bar is 0.1cm to 0.5cm; The spacing of adhering between bar is 2cm to 5cm; Adhere to bar surface finish coarse.Top frame and underframe form supporting construction, make to adhere to bar and are arranged into side by side the gapped inclined-plane of tool, and up-small and down-big structure can make whole device comparatively stable.And be convenient to a plurality of tiger spot cuttlefish artificial seed breeding methods to stack gradually up and down, receiving ovum, while going out pond, carrying, transportation, compared with prior art, there is great convenience.Having and only have top frame to have support bar can make this device stack one by one in the situation that being attached with tiger spot sea grape, and on pad between two adjacent tiger spot cuttlefish artificial seed breeding method support bars, be slightly less than the cystosepiment of top frame, the bar that adheres in the time of can making a plurality of tiger spot cuttlefish artificial seed breeding methods stack between upper and lower two these devices has gap, very practical when ovum in enormous quantities goes out pond, transportation.
Clamping-rope type cultivates the device of throwing something and feeding and has rope made of hemp body; Rope made of hemp body has two rope strands that can untwist and form 1 to 10 centimetre of ring that untwists; Rope made of hemp body two ends are provided with fixator; Rope made of hemp body tip is connected with pouring weight; Fixator is distortion bonding jumper or the symmetrical sheet metal being fixedly installed with clamping action; Rope strand has spin, and the loopback that can will untwist under the acting in conjunction of pouring weight is multiple.Rope made of hemp body is twisted and is formed by two rope strands, and these two rope strands have the spin with rope made of hemp body equidirectional, the active force that can just the ring that untwists be had gathering, be replied when the ring that untwists slightly form.In conjunction with the tractive of pouring weight self gravitation, in the time of making to untwist ring negligible amounts, still there is stronger clamping restoring force again.Fixator is fixed on the two ends of rope made of hemp body, can prevent that rope strand and the rope made of hemp body from untwisting.On folder, the rope strand of fish rocks in water, and the freezing fish on it is also just as being, and now cuttlefish is liked ediblely, can greatly increase cuttlefish grazing rate, reduces residual bait, improves cuttlefish growth rate.Hatching reinforced solution contains by weight: 0.01-5 part EDTA, 0.075-0.15 part (S)-3-(aminomethyl)-5-methylhexanoic acid, 1-5 part CaCl 2, 0.2-0.5 part MgCl 2, 1000 parts of water.
Because the present invention has adopted that parent is supported temporarily, the collection of ovum is, the step of the hatching of ovum, larval culture, use the clamping-rope type cultivation device fresh and alive small fish shrimp of throwing something and feeding of throwing something and feeding, improved the availability of bait, and grazing rate is also more excellent compared with prior art, contribute to parent to build up health, and can effectively prevent from ruining because of the water quality that residual bait causes.Tiger spot cuttlefish is adopted egg apparatus and has the attached ovum rate higher compared with prior art, and be easy to transportation and shift, simultaneously, owing to having adopted, by adopt egg apparatus with the tiger spot cuttlefish of ovum string, rest in air the processing mode of 1-3 minute, when the water of ovum string list face is drained, can stimulate the egg membrane surface of tiger spot cuttlefish, compared with routine techniques means, have higher membrane rate.Thereby the present invention has advantages of simple operation, parent loss is little, ovum grain membrane rate is high, water pollution is little, be suitable for large-scale production.
Accompanying drawing explanation
Fig. 1 is embodiment of the present invention sequence of steps schematic diagram;
Fig. 2 is that tiger spot cuttlefish of the present invention is adopted egg apparatus structural representation;
Fig. 3 is that clamping-rope type of the present invention cultivates the apparatus structure schematic diagram of throwing something and feeding.
Embodiment
Below in conjunction with attached embodiment, the present invention is described in further detail.
Drawing reference numeral explanation: top frame 11, support bar 111, underframe 12, bracing piece 13, adhere to bar 14, rope made of hemp body 21, rope strand 211, ring 212, fixator 22, pouring weight 23 untwist.
Embodiment: referring to figs. 1 through Fig. 3, tiger spot cuttlefish artificial seed breeding method, it comprises following step:
Figure 833401DEST_PATH_IMAGE002
parent is supported temporarily: parent is put into cement pit and support temporarily, parent breeding density is 0.5-2/square metre; Cement pit degree of depth 2-3 rice, the fine sand of paving 10-20 cm thick at the bottom of pond; Supporting seawater salinity is temporarily 28-30 ‰, and water temperature is 24-25 ℃, the preferred salinity 35 ‰ of the present embodiment, 20 ℃ of water temperatures.Keep perennial draingage, shading, inflation, keeps quite, and adopts the clamping-rope type cultivation device fresh and alive small fish shrimp of throwing something and feeding of throwing something and feeding; Clamping-rope type cultivates the availability that the device of throwing something and feeding can improve bait, and grazing rate is also more excellent compared with prior art, contributes to parent to build up health, and can effectively prevent from ruining because of the water quality that residual bait causes.
Figure 563591DEST_PATH_IMAGE004
the collection of ovum: by bosom ovum parent by putting into spawning pond, the area 20-25 square metre of spawning pond, depth of water 1.5-3 rice, the present embodiment preferably adopts 25 square metres of spawning ponds, 2 meters of the depth of waters.In the bottom in pond, place tiger spot cuttlefish and adopt egg apparatus; Parent breeding density 1-2/square metre, adopt in egg apparatus replacement water body and adopt egg apparatus with the tiger spot cuttlefish of ovum string every day with the tiger spot cuttlefish of cleaning, and the tiger spot cuttlefish with ovum string of collecting is adopted to egg apparatus rest on 1-3 minute in air, then spray hatching reinforced solution, and standing 1-3 minute again; Tiger spot cuttlefish is adopted egg apparatus and has the attached ovum rate higher compared with prior art, and be easy to transportation and shift, simultaneously, owing to having adopted, by adopt egg apparatus with the tiger spot cuttlefish of ovum string, rest in air the processing mode of 1-3 minute, when the water of ovum string list face is drained, can stimulate the egg membrane surface of tiger spot cuttlefish, compared with routine techniques means, have higher membrane rate.
Figure 343328DEST_PATH_IMAGE006
the hatching of ovum: the tiger spot cuttlefish with ovum string of same batch is adopted to the concentrated water middle part, hatchery that is suspended on of egg apparatus and hatch, ovum granule density is 300-350/cubic metre; The area 10-15 square metre of hatchery, the dark 1.6-2 rice in pond, 1 every square metre, gas stone, micro-inflation;
Figure 800854DEST_PATH_IMAGE008
larval culture: the young hatches the rear oppossum shrimp of directly throwing something and feeding, and illumination is 600-800 lux, micro-inflation, noise keeps, below 70 decibels, when seedling rouge, growing up after 2 centimetres, shifts out seedling outward.
Tiger spot cuttlefish is adopted egg apparatus and has top frame 11 and underframe 12; Two sides that top frame 11 is relative with underframe 12 have bracing piece 13; Top frame 11 is also evenly equipped with the parallel flexible bar 114 that adheres to that has in gap with underframe 12 sides; Top frame 11 has support bar 111; Adhere to bar 14 for the nylon plastic(s) of sheet; The width that adheres to bar 14 is 0.5cm to 1.5cm; The thickness that adheres to bar 14 is 0.1cm to 0.5cm; The spacing of adhering between bar 14 is 2cm to 5cm; Adhere to bar 14 surface finish coarse.
Clamping-rope type cultivates the device of throwing something and feeding and has rope made of hemp body 21; Rope made of hemp body 21 has two rope strands 211 that can untwist and form 1 to 10 centimetre of ring 212 that untwists; Rope made of hemp body 21 two ends are provided with fixator 22; Rope made of hemp body 21 ends are connected with pouring weight 23; Fixator 22 is for having distortion bonding jumper or the symmetrical sheet metal being fixedly installed of clamping action; Rope strand 211 has spin, and the ring 212 that can will untwist under the acting in conjunction of pouring weight 23 is replied.Hatching reinforced solution contains by weight: 0.01-5 part EDTA, 0.075-0.15 part (S)-3-(aminomethyl)-5-methylhexanoic acid, 1-5 part CaCl 2, 0.2-0.5 part MgCl 2, 1000 parts of water.The present embodiment is 0.1 part of EDTA, 0.075 part of (S)-3-(aminomethyl)-5-methylhexanoic acid, 1 CaCl preferably 2, 0.5 part of MgCl 2, 1000 parts of water.Hatching reinforced solution can clean ovum grain pellicle preferably, can effectively improve and ovum rate simultaneously.
This method, utilize hatching initial stage cuttlefish individuality larger, the feature of the larger individual living body bait of can ingesting, avoids conventional cuttlefish and cultivates conventional bait series such as adopting wheel animalcule-halogen worm-copepoda-oppossum shrimp, and adopt from the beginning oppossum shrimp to throw something and feed, be aided with a small amount of small fish, shrimp.Can make the seed phase cultivating survival rate of tiger spot cuttlefish reach 40%; Substantially can meet the needs that extensive artificial seed cultivates.
Although described the present invention in conjunction with preferred embodiment; so it is not in order to limit the present invention; any those skilled in the art; without departing from the spirit and scope of the present invention; can implement to the theme of listing displacement and the modification of various changes, coordinate here, so protection scope of the present invention is as the criterion when the scope of the claim restriction depending on proposed.

Claims (4)

1. tiger spot cuttlefish artificial seed breeding method, is characterized in that comprising following step:
Figure 2013105229614100001DEST_PATH_IMAGE002
parent is supported temporarily: parent is put into cement pit and support temporarily, parent breeding density is 0.5-2/square metre; Cement pit degree of depth 2-3 rice, the fine sand of paving 10-20 cm thick at the bottom of pond; Temporarily supporting seawater salinity is 28-30 ‰, and water temperature is 24-25 ℃, keeps perennial draingage, shading, and inflation, keeps quite, and adopts the clamping-rope type cultivation device fresh and alive small fish shrimp of throwing something and feeding of throwing something and feeding;
Figure 2013105229614100001DEST_PATH_IMAGE004
the collection of ovum: by bosom ovum parent by putting into spawning pond, the area 20-25 square metre of spawning pond, depth of water 1.5-3 rice, places tiger spot cuttlefish and adopts egg apparatus in the bottom in pond; Parent breeding density 1-2/square metre, adopt in egg apparatus replacement water body and adopt egg apparatus with the tiger spot cuttlefish of ovum string every day with the tiger spot cuttlefish of cleaning, and the tiger spot cuttlefish with ovum string of collecting is adopted to egg apparatus rest on 1-3 minute in air, then spray hatching reinforced solution, and standing 1-3 minute again;
Figure 2013105229614100001DEST_PATH_IMAGE006
the hatching of ovum: the tiger spot cuttlefish with ovum string of same batch is adopted to the concentrated water middle part, hatchery that is suspended on of egg apparatus and hatch, ovum granule density is 300-350/cubic metre; The area 10-15 square metre of hatchery, the dark 1.6-2 rice in pond, every square metre of 1-2 of gas stone, micro-inflation;
Figure 2013105229614100001DEST_PATH_IMAGE008
larval culture: the young hatches the rear oppossum shrimp of directly throwing something and feeding, and illumination is 600-800 lux, micro-inflation, noise keeps, below 70 decibels, when seedling rouge, growing up after 2 centimetres, shifts out seedling outward.
2. tiger spot cuttlefish artificial seed breeding method according to claim 1, is characterized in that: described tiger spot cuttlefish is adopted egg apparatus and has top frame (11) and underframe (12); Two sides that described top frame (11) is relative with underframe (12) have bracing piece (13); Described top frame (11) is also evenly equipped with the parallel flexible bar (114) that adheres to that has in gap with underframe (12) side; Described top frame (11) has support bar (111); Described adheres to bar (14) for the nylon plastic(s) of sheet; The described width that adheres to bar (14) is 0.5cm to 1.5cm; The described thickness that adheres to bar (14) is 0.1cm to 0.5cm; The described spacing of adhering between bar (14) is 2cm to 5cm; Described bar (14) surface finish of adhering to is coarse.
3. tiger spot cuttlefish artificial seed breeding method according to claim 1, is characterized in that: described clamping-rope type cultivates the device of throwing something and feeding and has rope made of hemp body (21); Described rope made of hemp body (21) has two rope strands (211) that can untwist and form 1 to 10 centimetre of ring (212) of untwisting; Described rope made of hemp body (21) two ends are provided with fixator (22); Described rope made of hemp body (21) end is connected with pouring weight (23); Described fixator (22) is for having distortion bonding jumper or the symmetrical sheet metal being fixedly installed of clamping action; Described rope strand (211) has spin, and can under the acting in conjunction of pouring weight (23), the described ring (212) that untwists be replied.
4. tiger spot cuttlefish artificial seed breeding method according to claim 1, is characterized in that: described hatching reinforced solution contains by weight: 0.01-5 part EDTA, 0.075-0.15 part (S)-3-(aminomethyl)-5-methylhexanoic acid, 1-5 part CaCl 2, 0.2-0.5 part MgCl 2, 1000 parts of water.
CN201310522961.4A 2013-10-30 2013-10-30 Tiger spot cuttlefish artificial seed breeding method Expired - Fee Related CN103548732B (en)

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CN104273074A (en) * 2014-10-31 2015-01-14 岭南师范学院 Method for cultivating inkfish fries
CN104472406A (en) * 2014-12-03 2015-04-01 宁波大学 Method for cultivating tiger-spot cuttlefishes in indoor cement pond
CN105994016A (en) * 2015-12-09 2016-10-12 江苏省海洋资源开发研究院(连云港) Method for harvesting and temporarily culturing sepia esculenta in cuttlefish-shrimp polyculture pond
CN108935257A (en) * 2018-09-22 2018-12-07 淮海工学院 A kind of shrimp ponds net cage combined breeding method

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104273074A (en) * 2014-10-31 2015-01-14 岭南师范学院 Method for cultivating inkfish fries
CN104472406A (en) * 2014-12-03 2015-04-01 宁波大学 Method for cultivating tiger-spot cuttlefishes in indoor cement pond
CN105994016A (en) * 2015-12-09 2016-10-12 江苏省海洋资源开发研究院(连云港) Method for harvesting and temporarily culturing sepia esculenta in cuttlefish-shrimp polyculture pond
CN105994016B (en) * 2015-12-09 2020-01-14 江苏省海洋资源开发研究院(连云港) Method for harvesting and temporarily culturing sepia esculenta in cuttlefish and shrimp polyculture pond
CN108935257A (en) * 2018-09-22 2018-12-07 淮海工学院 A kind of shrimp ponds net cage combined breeding method

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