CN109430117A - Improve quasi- mesh cuttlefish reproductive capacity method - Google Patents
Improve quasi- mesh cuttlefish reproductive capacity method Download PDFInfo
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- CN109430117A CN109430117A CN201810978633.8A CN201810978633A CN109430117A CN 109430117 A CN109430117 A CN 109430117A CN 201810978633 A CN201810978633 A CN 201810978633A CN 109430117 A CN109430117 A CN 109430117A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses improve quasi- mesh cuttlefish reproductive capacity method, the good quasi- mesh cuttlefish of combo is temporarily supported in the indoor culture pond for being equipped with attached ovum base, carry out stimulation by running water, bait throwing in contains the bait of fermented bean dregs, after quasi- mesh cuttlefish parent natural spawning, collects fertilized eggs and carry out artificial incubation, then cultivate the seedling hatched in seedling pond, it launches after the long qualification of seedling trunk into aquaculture pond and cultivates, fermented bean dregs are fermented with the Lactobacillus rhamnosus containing tocopherol and cysteine and obtained.Have the beneficial effect that the method for the present invention can improve grazing rate, utilization rate and the immunity of organisms of quasi- mesh cuttlefish parent, fertilized eggs can be made to be sticked together, it is not easy to be broken up by water flow, improve bacteriostasis, Fertilized quality and the hatching rate of fertilized eggs, inhibit the growth of bacterium, it is final to improve quasi- mesh cuttlefish fertility.
Description
Technical field
The present invention relates to technical field of aquaculture, intend mesh cuttlefish reproductive capacity method more particularly, to improving.
Background technique
Cuttlefish belongs to Mollusca, and Cephalopoda, Decapodiformes, Sepiida is the type of siphonopods most potentiality to be exploited
One of, delicious meat, edible part accounts for 90% or more；Its gonad can be processed into " snakeheaded fish fringe " and " snakehead's egg "；In addition its
Cuttlebone and ink sac have blood coagulation, antitumor, anticancer and other effects.Therefore cuttlefish is liked by masses deeply, is the important ocean in China
Fishery resources.In recent years, both at home and abroad to the existing master for compared with in-depth study, being caught, being developed and used at present of cuttlefish resource distribution
There is kind, China is higher with golden cuttlefish, Sepiella maindroni, cuttlefish, quasi- mesh cuttlefish, tiger spot cuttlefish Isoquant.But due to catching
Fishing is excessively and reasons, the cuttlefish resources such as marine environment deteriorates obviously fail, and can not form fishing season.Breeding period is cuttlefishes Life Cycle
The important stage of phase, other than the huge cuttlefish of Australia, most of cuttlefish breeding period hardly clusterings, but they have breeding migration
The phenomenon that, only one breeding period of general cuttlefishes, as the golden cuttlefish of natural waters, Sepiella maindroni belong to this.But
Under the conditions of artificial breeding, due to the change of environmental factor, Sepiella maindroni can be bred 2 times.Parent fish brood amount determines thereafter
The quality and quantity in generation restricts the height of quantity, quality and the diversity level of recruitment stock.The fish brood amount of different cuttlefishes
There is apparent difference, if Sepiella maindroni fish brood amount is generally 1000-3000, golden cuttlefish is generally 500-1500,
Tiger spot cuttlefish is generally 500-3000, and intending mesh cuttlefish eggs on average amount is 354.In order to restore, develop and utilize cuttlefish money
Source, foreign scholar is relatively early to have carried out the research of cuttlefish artificial breeding.China mainly collects the breeding and cultural technique research of cuttlefish
In in Sepiella maindroni, golden cuttlefish and tiger spot cuttlefish, and it is less to the breeding and cultural technique research of quasi- mesh cuttlefish.
Quasi- mesh cuttlefish, which enters breeding period, apparent courtship ritual, and male is often fought because striving occasionally；When seeking a spouse, crow
Thief is without feed phenomenon.After seeking a spouse successfully, to avoid female by the interference of other males, for male with the behavior of escorting, this can
It can be a kind of mating strategy for improving fertility rate and hatchability.When mating, spermatophore is delivered to receiving for female with hectocotylized arm by male
In seminal vesicle, after fertilization, female individuals select fixed algae, branch, shell etc. to lay eggs as attachment base.Quasi- mesh cuttlefish table
It now lays eggs for collective, coheres and form botryoidal public breeding area on same ovum chain, eggs on average amount is 354, simultaneously
It is similar to Sepiella maindroni, intend mesh cuttlefish also without significantly protecting ovum behavior, adds overfishing children cuttlefish, overwintering cuttlefish
And the oviposition parent of breeding period, cause quasi- mesh cuttlefish resource recruitment stock to receive and seriously threaten, resource is caused to fail.So grinding
Study carefully and how to improve quasi- mesh cuttlefish reproductive capacity method and can enrich quasi- mesh cuttlefish Biological Knowledge, carries out on a large scale for further quasi-
The artificial breeding of mesh cuttlefish provides theoretical foundation, to promotion artificial breeding technique, promotion large-scale cultivation and Stock resoures
Restore all significant.
Summary of the invention
The purpose of the present invention is to provide a kind of grazing rate, utilization rate and immunity of organism that can improve quasi- mesh cuttlefish parent
Power can make fertilized eggs be sticked together, and be not easy to be broken up by water flow, improve the bacteriostasis, Fertilized quality and hatching of fertilized eggs
Rate inhibits the growth of bacterium, and mesh cuttlefish reproductive capacity method is intended in the raising for improving quasi- mesh cuttlefish fertility.
The present invention in view of the above technology in the problem of mentioning, the technical solution taken are as follows:
Quasi- mesh cuttlefish reproductive capacity method is improved, the good quasi- mesh cuttlefish of combo is temporarily supported in the indoor culture pond for being equipped with attached ovum base
In, carry out stimulation by running water, bait throwing in contains the bait of fermented bean dregs, after quasi- mesh cuttlefish parent natural spawning, collect fertilized eggs into
Then row artificial incubation cultivates the seedling hatched in seedling pond, launch after the long qualification of seedling trunk into aquaculture pond and cultivate,
Fermented bean dregs are fermented with the Lactobacillus rhamnosus containing tocopherol and cysteine and are obtained.Quasi- mesh cuttlefish can be improved in the method for the present invention
Grazing rate, utilization rate and the immunity of organisms of parent improve the expression for participating in promoting oocyte maturation factor gene, and drop
The low expression for inhibiting the oocyte maturation factor, promotes the maturation of egg mother cell, increases egg laying amount, rate of fertilization and hatching
Rate, and then improve the reproductive capacity of quasi- mesh cuttlefish；Fertilized eggs can be made to be sticked together, be not easy to be broken up by water flow, improve fertilized eggs
Bacteriostasis, Fertilized quality and hatching rate；Attached ovum base used has good flexibility, antibiotic property, adhesion property and affine
Performance can effectively inhibit the growth of bacterium, avoid quasi- mesh cuttlefish injured and influence to lay eggs, enable fertilized eggs attached securely
On attached ovum base, improve the archiblast amount of fertilized eggs, and then improve hatching rate, it is final to improve quasi- mesh cuttlefish fertility.
Preferably, attached ovum base is the polyethylene mesh sheet of mesh 25-30mm.The attached ovum base not will cause the injured simultaneously shadow of cuttlefish
Oviposition is rung, reusable, resistance to long-time is impregnated in the seawater, does not ruin water quality, attached ovum effect is good, when can shorten oviposition
Between, male and female post-coitum 1min, female just starts to invest ovum on attached ovum base, and attachment is uniformly secured, improves egg laying amount, attached ovum amount is most
Mostly up to 809/piece, the fertilized eggs mildew or necrosis of quasi- mesh cuttlefish can also be avoided, improves archiblast amount, and then improve hatching
Rate, it is final to improve quasi- mesh cuttlefish fertility.
Further preferably, attached ovum base the preparation method comprises the following steps: by weight ratio be 100:0.1-3 polyethylene and epsilon-polylysine
In being sufficiently kneaded 8-12min under 158-163 DEG C, 280-350rpm on double rod kneading machines, the composite material that then will be blended
It is put into mold, the hot pressing 4-6min at 158-163 DEG C, 8-12MPa is transferred on vulcanizing press, is then cold-pressed at room temperature, it is cold
After the completion of pressure, it is removed from the molds to get the attached ovum base of polyethylene mesh sheet.The attached ovum base of the polyethylene mesh sheet has both polyethylene and ε-is poly-
The function of lysine has excellent antibacterial effect, can effectively inhibit the growth of bacterium, and plays in a long time good
Antibacterial ability, be not easy to be polluted by cuttlefish excreta or prepared Chinese ink, avoid the fertilized eggs mildew or necrosis of quasi- mesh cuttlefish, improve fertilization
The archiblast amount of ovum assists to improve quasi- mesh cuttlefish fertility, while the addition of the epsilon-polylysine does not influence polyethylene mesh sheet
Mechanical property, and have no adverse effects to quasi- mesh cuttlefish fertilized eggs.
Still more preferably, the D-Lys of 0.03-0.06wt% are contained in epsilon-polylysine.D- relies ammonia in reaction system
The presence of acid can be opened epsilon-polylysine is fully dispersed in polyethylene, enhance epsilon-polylysine and polyethylene compatibility and
Blended improves the service performance and biocompatibility of attached ovum base, has no adverse effects to quasi- mesh cuttlefish fertilized eggs, can assist to improve
Quasi- mesh cuttlefish fertility, while can cooperate with epsilon-polylysine, weaken the active force between polyethylene, hinders crystallization
The formation of structure reduces the crystallinity of polyethylene, so that attached ovum base has good flexibility, avoids quasi- mesh cuttlefish injured and shadow
Oviposition is rung, in addition, the addition of D-Lys helps to promote the cell adherence performance and affine performance of attached ovum base, so that fertilized eggs
It can be securely attached on attached ovum base, and then improve hatching rate, it is final to improve quasi- mesh cuttlefish fertility.
Preferably, the specific fermentation step of fermented bean dregs are as follows: dregs of beans is ground to 80-120 mesh, adding water to water content is
45-53%, high pressure sterilization 20-30min, cooling after mixing, and inoculum concentration is that 3-5% is inoculated with Lactobacillus rhamnosus, in 35-40
Ferment 1-2d at DEG C, and freeze-drying crushed 200-300 mesh to get fermented bean dregs.It can be improved in fermentation process
The content of crude fat, crude protein, amino acid and phosphorus in dregs of beans, and macro-molecular protein is resolved into small protein and activity
Peptide improves quasi- mesh cuttlefish to the digestive utilization ratio and immunity of organisms of albumen, meanwhile, the part anti-nutritional factors quilt in dregs of beans
It eliminates, increases bioactie agent and antibacterial ability in dregs of beans, improve the palatability of dregs of beans, in addition, the sandlwood in fermented bean dregs
The expression for participating in promoting oocyte maturation factor gene can be improved in sugared lactobacillus, and reduces inhibition oocyte maturation
The expression of the factor promotes the maturation of egg mother cell, increases egg laying amount, rate of fertilization and hatching rate, and then improves quasi- mesh cuttlefish
Reproductive capacity, and some enzymes for enhancing Host Digestions, such as amylase, protease and lipase are produced in host intestine, promote place
The digestibility of main organic substance and protein can also provide some required growth factors, vitamin or amino acid, influence machine
The energetic supersession and energy balance of body, therefore, fermented bean dregs can be improved the grazing rate of quasi- mesh cuttlefish, utilization rate, immunity of organism
Power and reproductive capacity.
Preferably, tocopherol is the 3.5-5.6% of Lactobacillus rhamnosus weight, cysteine is Lactobacillus rhamnosus weight
The 0.8-1.5% of amount.Tocopherol and the special of cysteine can provide sufficient carbon source in the presence of one side for Lactobacillus rhamnosus
And nitrogen source, promote the growth of lactic acid bacteria, extend the logarithmic phase of Lactobacillus rhamnosus, so that Lactobacillus rhamnosus is metabolized out more
The metabolites such as organic acid, enzyme, exocellular polysaccharide change the molecular structure inside dregs of beans, so that dregs of beans is with more hydrophilic
Group is conducive to dregs of beans and is absorbed and used；On the other hand it can be absorbed and utilized, be improved in grey matter and white matter by quasi- mesh cuttlefish
The content of FMRFamide increases the secretion yield with the string effect of adhesion ovum in spawn tangled gland and secondary spawn tangled gland, makes
Fertilized eggs are sticked together, and are not easy to be broken up by water flow, improve hatching rate, and can stimulate female parent secondary spawn tangled gland lumen in energy
The bacterium mass propagation for producing antibiotic, is then attached on the tertiary egg envelope of ovum with secretion, improves the bacteriostasis of fertilized eggs, mention
The hatching rate of high fertilized eggs；In a word there is the hatching that can be improved quasi- mesh cuttlefish fertilized eggs in tocopherol and the special of cysteine
Rate.
Preferably, the ingredient and its parts by weight of bait are as follows: 18-28 parts of fermented bean dregs, 10-20 parts of fish meal, bone meal 10-20
Part, 7-15 parts of oppossum shrimp powder, 0.002-0.004 parts of vitamin A, 0.001-0.002 parts of vitamin E, vitamin C 0.002-
0.005 part, 3-7 parts of vegetable oil, 0.2-0.4 parts of citric acid, 0.1-0.3 parts of phytase.
Preferably, the condition of parents culture are as follows: water temperature is 20-25 DEG C, and seawater salinity 25-30, the photoperiod, L:D was
4:18-25, the flow velocity of stimulation by running water is 5-8m/min, time 10-15min, day bait throwing in 2-4 times, and total feeding volume is parent
0.3-0.6 times of weight.
Preferably, parent select and combo specifically: according to the natural propagation method of quasi- mesh cuttlefish, screening is strong respectively
Health, sexually matured male and female intend mesh cuttlefish and make parent, and then the graceful formula sepiella maindroni de Rochebrune of male and female carries out combo by 2-4:1.
Preferably, artificial incubation specifically: fertilized eggs are placed in culture pond, control culture pond water temperature is 20-25
DEG C, seawater salinity 25-30, water-soluble oxygen amount >=6mg/L, brooding time 20-35d, hatchery top cover single layer sunshade net.
Compared with the prior art, the advantages of the present invention are as follows: 1) the method for the present invention can be improved the ingesting of quasi- mesh cuttlefish parent
Rate, utilization rate and immunity of organisms improve the expression for participating in promoting oocyte maturation factor gene, and reduce inhibition ovum
The expression of mother cell maturation factor promotes the maturation of egg mother cell, increases egg laying amount, rate of fertilization and hatching rate, and then improve
The reproductive capacity of quasi- mesh cuttlefish；2) the method for the present invention can make fertilized eggs be sticked together, and be not easy to be broken up by water flow, improve fertilized eggs
Bacteriostasis, Fertilized quality and hatching rate；3) attached ovum base used in the method for the present invention has good flexibility, antibiotic property, glues
Attached performance and affine performance can effectively inhibit the growth of bacterium, avoid quasi- mesh cuttlefish injured and influence to lay eggs, so that fertilized eggs
It can be securely attached on attached ovum base, improve the archiblast amount of fertilized eggs, and then improve hatching rate, the final quasi- mesh cuttlefish of raising is numerous
Grow ability.
Detailed description of the invention
The test result of average attached ovum amount, hatching rate, survival rate in Fig. 1 embodiment 6.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
Quasi- mesh cuttlefish reproductive capacity method is improved, the good quasi- mesh cuttlefish of combo is temporarily supported in the indoor culture pond for being equipped with attached ovum base
In, carry out stimulation by running water, bait throwing in contains the bait of fermented bean dregs, after quasi- mesh cuttlefish parent natural spawning, collect fertilized eggs into
Then row artificial incubation cultivates the seedling hatched in seedling pond, launch after the long qualification of seedling trunk into aquaculture pond and cultivate,
Fermented bean dregs are fermented with the Lactobacillus rhamnosus containing tocopherol and cysteine and are obtained.Quasi- mesh cuttlefish parent can be improved in this method
Grazing rate, utilization rate and immunity of organisms, improve the expression for participating in promoting oocyte maturation factor gene, and reduce
The expression for inhibiting the oocyte maturation factor promotes the maturation of egg mother cell, increases egg laying amount, rate of fertilization and hatching rate, into
And improve the reproductive capacity of quasi- mesh cuttlefish；Fertilized eggs can be made to be sticked together, be not easy to be broken up by water flow, improve the antibacterial energy of fertilized eggs
Power, Fertilized quality and hatching rate；Attached ovum base used has good flexibility, antibiotic property, adhesion property and affine performance, energy
Enough growths for effectively inhibiting bacterium avoid quasi- mesh cuttlefish injured and influence to lay eggs, fertilized eggs are securely attached to attached
On ovum base, the archiblast amount of fertilized eggs is improved, and then improve hatching rate, it is final to improve quasi- mesh cuttlefish fertility.
Attached ovum base is the polyethylene mesh sheet of mesh 30mm.The attached ovum base not will cause cuttlefish injury and influence to lay eggs, and can weigh
Multiple to utilize, resistance to long-time is impregnated in the seawater, does not ruin water quality, attached ovum effect is good, can shorten egg-laying time, male and female post-coitum
1min, female just start to invest ovum on attached ovum base, attachment uniformly it is secured, improve egg laying amount, attached ovum amount at most up to 809/
Piece can also avoid the fertilized eggs mildew or downright bad of quasi- mesh cuttlefish, improve archiblast amount, and then improve hatching rate, and final improve is intended
Mesh cuttlefish fertility.
Above-mentioned attached ovum base the preparation method comprises the following steps: the polyethylene and epsilon-polylysine that weight ratio is 100:0.1 are mixed in double rods
12min is sufficiently kneaded in mill under 163 DEG C, 280rpm, the composite material being blended then is put into mold, is transferred to plate
On vulcanizer at 158 DEG C, 12MPa hot pressing 4min, be then cold-pressed, after the completion of cold pressing, be removed from the molds to get poly- at room temperature
The attached ovum base of ethylene mesh sheet.Contain the D-Lys of 0.06wt% in above-mentioned epsilon-polylysine.D-Lys deposits in reaction system
It can open epsilon-polylysine is fully dispersed in polyethylene, enhancing epsilon-polylysine and the compatibility of polyethylene and be blended
Property, the service performance and biocompatibility of attached ovum base are improved, is had no adverse effects to quasi- mesh cuttlefish fertilized eggs, can assist to improve quasi- mesh
Cuttlefish fertility, while can cooperate with epsilon-polylysine, weaken the active force between polyethylene, hinders crystalline texture
Formation, reduce the crystallinity of polyethylene, so that attached ovum base has good flexibility, avoid quasi- mesh cuttlefish injured and influence to produce
Ovum enables fertilized eggs in addition, the addition of D-Lys helps to promote the cell adherence performance and affine performance of attached ovum base
It is securely attached on attached ovum base, and then improves hatching rate, it is final to improve quasi- mesh cuttlefish fertility.The attached ovum of polyethylene mesh sheet
Base has both the function of polyethylene and epsilon-polylysine, has excellent antibacterial effect, can effectively inhibit the growth of bacterium, and
Good antibacterial ability is played in long period, is not easy to be polluted by cuttlefish excreta or prepared Chinese ink, avoids the fertilized eggs of quasi- mesh cuttlefish
Mildew or necrosis, improve the archiblast amount of fertilized eggs, assist to improve quasi- mesh cuttlefish fertility, while the addition of the epsilon-polylysine
The mechanical property of polyethylene mesh sheet is not influenced, and is had no adverse effects to quasi- mesh cuttlefish fertilized eggs.
The specific fermentation step of fermented bean dregs are as follows: dregs of beans is ground to 80 mesh, adding water to water content is 53%, is uniformly mixed
High pressure sterilization 20min afterwards, cooling, inoculum concentration is 5% inoculation Lactobacillus rhamnosus, and ferment 2d at 35 DEG C, is freeze-dried, and is crushed
200 meshes are crossed to get fermented bean dregs.It can be improved crude fat in dregs of beans, crude protein, amino acid and phosphorus in fermentation process
Content, and macro-molecular protein is resolved into small protein and active peptide, improves digestion of the quasi- mesh cuttlefish to albumen
Utilization rate and immunity of organisms, meanwhile, the part anti-nutritional factors in dregs of beans is eliminated, increase dregs of beans in bioactie agent and
Antibacterial ability improves the palatability of dregs of beans, promotes ovum female thin in addition, participation can be improved in the Lactobacillus rhamnosus in fermented bean dregs
The expression of born of the same parents' maturation factor gene, and the expression for inhibiting the oocyte maturation factor is reduced, promote the maturation of egg mother cell,
Increase egg laying amount, rate of fertilization and hatching rate, and then improves the reproductive capacity of quasi- mesh cuttlefish, and produce in host intestine
Enhance the enzyme of Host Digestion, such as amylase, protease and lipase, promotes the digestibility of host microorganisms matter and protein, may be used also
To provide some required growth factors, vitamin or amino acid, the energetic supersession and energy balance of body are influenced, therefore, hair
Ferment dregs of beans can be improved grazing rate, utilization rate, immunity of organisms and the reproductive capacity of quasi- mesh cuttlefish.
Tocopherol is the 5.6% of Lactobacillus rhamnosus weight, and cysteine is the 0.8% of Lactobacillus rhamnosus weight.It is raw
Sufficient carbon source and nitrogen source can be provided in the presence of one side for Lactobacillus rhamnosus by educating phenol and the special of cysteine, promote lactic acid bacteria
Growth, extend the logarithmic phase of Lactobacillus rhamnosus, so that Lactobacillus rhamnosus is metabolized out more organic acids, enzyme, extracellular
The metabolites such as polysaccharide change the molecular structure inside dregs of beans, so that dregs of beans has more hydrophilic radicals, are conducive to dregs of beans quilt
It is absorbed and utilized；On the other hand it can be absorbed and utilized by quasi- mesh cuttlefish, improve the content of FMRFamide in grey matter and white matter, then make
It obtains the secretion yield with the string effect of adhesion ovum in spawn tangled gland and secondary spawn tangled gland to increase, so that fertilized eggs is sticked together, be not easy
Broken up by water flow, improve hatching rate, and can stimulate female parent secondary spawn tangled gland lumen in can produce antibiotic bacterium it is largely numerous
It grows, is then attached on the tertiary egg envelope of ovum with secretion, improve the bacteriostasis of fertilized eggs, improve the hatching rate of fertilized eggs；Always
Tocopherol and cysteine it is special exist can be improved the hatching rates of quasi- mesh cuttlefish fertilized eggs.
The ingredient and its parts by weight of bait are as follows: 28 parts of fermented bean dregs, 10 parts of fish meal, 20 parts of bone meal, 7 parts of oppossum shrimp powder, dimension life
0.004 part of plain A, 0.001 part of vitamin E, 0.005 part of vitamin C, 3 parts of vegetable oil, 0.4 part of citric acid, phytase 0.1
Part.
The condition of parents culture are as follows: water temperature is 25 DEG C, seawater salinity 25, and photoperiod L:D is 4:25, the stream of stimulation by running water
Speed is 5m/min, time 15min, and day bait throwing in 2 times, total feeding volume is 0.6 times of parent weight.
Parent select and combo specifically: according to the natural propagation method of quasi- mesh cuttlefish, screening health, sexal maturity respectively
Male and female intend mesh cuttlefish make parent, then the graceful formula sepiella maindroni de Rochebrune of male and female by 2:1 carry out combo.
Artificial incubation specifically: fertilized eggs are placed in culture pond, control culture pond water temperature is 20 DEG C, and seawater salinity is
30, water-soluble oxygen amount >=6mg/L, brooding time 20d, hatchery top cover single layer sunshade net.
Embodiment 2:
Quasi- mesh cuttlefish reproductive capacity method is improved, the good quasi- mesh cuttlefish of combo is temporarily supported in the indoor culture pond for being equipped with attached ovum base
In, carry out stimulation by running water, bait throwing in contains the bait of fermented bean dregs, after quasi- mesh cuttlefish parent natural spawning, collect fertilized eggs into
Then row artificial incubation cultivates the seedling hatched in seedling pond, launch after the long qualification of seedling trunk into aquaculture pond and cultivate,
Fermented bean dregs are fermented with the Lactobacillus rhamnosus containing tocopherol and cysteine and are obtained.
Attached ovum base is the polyethylene mesh sheet of mesh 27mm.
Above-mentioned attached ovum base the preparation method comprises the following steps: the polyethylene and epsilon-polylysine that weight ratio is 100:1.3 are mixed in double rods
10min is sufficiently kneaded in mill under 160 DEG C, 300rpm, the composite material being blended then is put into mold, is transferred to plate
On vulcanizer at 160 DEG C, 10MPa hot pressing 5min, be then cold-pressed, after the completion of cold pressing, be removed from the molds to get poly- at room temperature
The attached ovum base of ethylene mesh sheet.Contain the D-Lys of 0.04wt% in above-mentioned epsilon-polylysine.
The specific fermentation step of fermented bean dregs are as follows: dregs of beans is ground to 100 mesh, adding water to water content is 50%, and mixing is equal
High pressure sterilization 25min after even, cooling, inoculum concentration is 4% inoculation Lactobacillus rhamnosus, and ferment 1.5d at 38 DEG C, freeze-drying,
250 meshes be crushed to get fermented bean dregs.
Tocopherol is the 4.0% of Lactobacillus rhamnosus weight, and cysteine is the 1.1% of Lactobacillus rhamnosus weight.
The ingredient and its parts by weight of bait are as follows: 22 parts of fermented bean dregs, 15 parts of fish meal, 15 parts of bone meal, 10 parts of oppossum shrimp, vitamin
0.003 part of A, 0.0015 part of vitamin E, 0.003 part of vitamin C, 3-7 parts of vegetable oil, 0.3 part of citric acid, phytase 0.2
Part.
The condition of parents culture are as follows: water temperature is 22 DEG C, seawater salinity 28, and photoperiod L:D is 4:20, the stream of stimulation by running water
Speed is 6m/min, time 12min, and day bait throwing in 3 times, total feeding volume is 0.45 times of parent weight.
Parent select and combo specifically: according to the natural propagation method of quasi- mesh cuttlefish, screening health, sexal maturity respectively
Male and female intend mesh cuttlefish make parent, then the graceful formula sepiella maindroni de Rochebrune of male and female by 3:1 carry out combo.
Artificial incubation specifically: fertilized eggs are placed in culture pond, control culture pond water temperature is 22 DEG C, and seawater salinity is
28, water-soluble oxygen amount >=6mg/L, brooding time 30d, hatchery top cover single layer sunshade net.
Embodiment 3:
Quasi- mesh cuttlefish reproductive capacity method is improved, the good quasi- mesh cuttlefish of combo is temporarily supported in the indoor culture pond for being equipped with attached ovum base
In, carry out stimulation by running water, bait throwing in contains the bait of fermented bean dregs, after quasi- mesh cuttlefish parent natural spawning, collect fertilized eggs into
Then row artificial incubation cultivates the seedling hatched in seedling pond, launch after the long qualification of seedling trunk into aquaculture pond and cultivate,
Fermented bean dregs are fermented with the Lactobacillus rhamnosus containing tocopherol and cysteine and are obtained.
Attached ovum base is the polyethylene mesh sheet of mesh 25mm.
Above-mentioned attached ovum base the preparation method comprises the following steps: the polyethylene and epsilon-polylysine that weight ratio is 100:3 are kneaded in double rods
8min is sufficiently kneaded on machine under 158 DEG C, 350rpm, the composite material being blended then is put into mold, is transferred to plate sulphur
On change machine at 163 DEG C, 8MPa hot pressing 6min, be then cold-pressed, after the completion of cold pressing, be removed from the molds to get poly- second at room temperature
The attached ovum base of alkene mesh sheet.Contain the D-Lys of 0.03wt% in above-mentioned epsilon-polylysine.
The specific fermentation step of fermented bean dregs are as follows: dregs of beans is ground to 120 mesh, adding water to water content is 45%, and mixing is equal
High pressure sterilization 30min after even, cooling, inoculum concentration is 3% inoculation Lactobacillus rhamnosus, and ferment 1d at 40 DEG C, freeze-drying, powder
Broken 300 mesh of mistake is to get fermented bean dregs.
Tocopherol is the 3.5% of Lactobacillus rhamnosus weight, and cysteine is the 1.5% of Lactobacillus rhamnosus weight.
The ingredient and its parts by weight of bait are as follows: 18 parts of fermented bean dregs, 20 parts of fish meal, 10 parts of bone meal, 15 parts of oppossum shrimp powder, dimension life
0.002 part of plain A, 0.002 part of vitamin E, 0.002 part of vitamin C, 7 parts of vegetable oil, 0.2 part of citric acid, phytase 0.3
Part.
The condition of parents culture are as follows: water temperature is 20 DEG C, seawater salinity 30, and photoperiod L:D is 4:18, the stream of stimulation by running water
Speed is 8m/min, time 10min, and day bait throwing in 4 times, total feeding volume is 0.3 times of parent weight.
Parent select and combo specifically: according to the natural propagation method of quasi- mesh cuttlefish, screening health, sexal maturity respectively
Male and female intend mesh cuttlefish make parent, then the graceful formula sepiella maindroni de Rochebrune of male and female by 4:1 carry out combo.
Artificial incubation specifically: fertilized eggs are placed in culture pond, control culture pond water temperature is 25 DEG C, and seawater salinity is
25, water-soluble oxygen amount >=6mg/L, brooding time 35d, hatchery top cover single layer sunshade net.
Embodiment 4:
Improve quasi- mesh cuttlefish reproductive capacity method, attached ovum base the preparation method comprises the following steps: be free of D-Lys in epsilon-polylysine,
He and implementation 2 are completely the same.
Embodiment 5:
Improve quasi- mesh cuttlefish reproductive capacity method, Lactobacillus rhamnosus hair of the fermented bean dregs without tocopherol and cysteine
Ferment and obtain, other and implement 2 completely the same.
Embodiment 6:
Setting embodiment 2 is test group, and embodiment 4 and embodiment 5 are respectively control group 1 and control group 2, is calculated average attached
Ovum amount (grain), hatching rate (%), survival rate (%).Calculated result is as shown in Figure 1.
As shown in Figure 1, attached ovum amount, hatching rate, the survival rate of test group are above control group 1 and control group 2, illustrate to implement
The quasi- mesh cuttlefish fertility of example 2 is higher than embodiment 4 and embodiment 5, and it is quasi- to further illustrate that the addition of epsilon-polylysine can improve
The attached ovum amount of mesh cuttlefish, and then improve its hatching rate；The special presence of tocopherol and cysteine can improve incubating for quasi- mesh cuttlefish
Rate and survival rate, it is final to improve quasi- mesh cuttlefish fertility.
Routine operation in operating procedure of the invention is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (10)
1. improving quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: temporarily support the good quasi- mesh cuttlefish of combo and be equipped with attached ovum base
In indoor culture pond, stimulation by running water is carried out, bait throwing in contains the bait of fermented bean dregs, after quasi- mesh cuttlefish parent natural spawning, receives
Collect fertilized eggs progress artificial incubation, then cultivate the seedling hatched in seedling pond, launches after the long qualification of seedling trunk to feeding
It grows in pond and cultivates, the fermented bean dregs are fermented with the Lactobacillus rhamnosus containing tocopherol and cysteine and obtained.
2. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the attached ovum base is mesh
The polyethylene mesh sheet of 25-30mm.
3. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the preparation of the attached ovum base
Method are as follows: by weight ratio be 100:0.1-3 polyethylene and epsilon-polylysine on double rod kneading machines in 158-163 DEG C, 280-
It is sufficiently kneaded 8-12min under 350rpm, the composite material being blended then is put into mold, is transferred on vulcanizing press
158-163 DEG C, hot pressing 4-6min under 8-12MPa, are then cold-pressed at room temperature, after the completion of cold pressing, are removed from the molds to get poly- second
The attached ovum base of alkene mesh sheet.
4. according to claim 3 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: in the epsilon-polylysine
D-Lys containing 0.03-0.06wt%.
5. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the tool of the fermented bean dregs
Body fermentation step are as follows: dregs of beans is ground to 80-120 mesh, adding water to water content is 45-53%, after mixing high pressure sterilization 20-
30min, cooling, inoculum concentration is that 3-5% is inoculated with Lactobacillus rhamnosus, and ferment 1-2d at 35-40 DEG C, and freeze-drying crushed
200-300 mesh is to get fermented bean dregs.
6. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the tocopherol is sandlwood
The 3.5-5.6% of sugared lactobacillus weight, the cysteine are the 0.8-1.5% of Lactobacillus rhamnosus weight.
7. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the ingredient of the bait and
Its parts by weight are as follows: 18-28 parts of fermented bean dregs, 10-20 parts of fish meal, 10-20 parts of bone meal, 7-15 parts of oppossum shrimp powder, vitamin A
0.002-0.004 parts, 0.001-0.002 parts of vitamin E, 0.002-0.005 parts of vitamin C, 3-7 parts of vegetable oil, citric acid
0.2-0.4 parts, 0.1-0.3 parts of phytase.
8. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the item of the parents culture
Part are as follows: it is 4:18-25 that water temperature, which is 20-25 DEG C, seawater salinity 25-30, photoperiod L:D, and the flow velocity of stimulation by running water is 5-8m/
Min, time 10-15min, day bait throwing in 2-4 times, total feeding volume are 0.3-0.6 times of parent weight.
9. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the parent select and
Combo specifically: according to the natural propagation method of quasi- mesh cuttlefish, screening health, sexually matured male and female intend mesh cuttlefish and make parent respectively
This, then the graceful formula sepiella maindroni de Rochebrune of male and female carries out combo by 2-4:1.
10. according to claim 1 improve quasi- mesh cuttlefish reproductive capacity method, it is characterised in that: the artificial incubation tool
Body are as follows: fertilized eggs are placed in culture pond, control culture pond water temperature is 20-25 DEG C, seawater salinity 25-30, water-soluble oxygen amount
>=6mg/L, brooding time 20-35d, hatchery top cover single layer sunshade net.
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Citations (5)
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|---|---|---|---|---|
| JPH10136828A (en) * | 1996-11-13 | 1998-05-26 | Asahi Chem Ind Co Ltd | Fish bank and substrate therefor |
| CN102823529A (en) * | 2012-09-17 | 2012-12-19 | 湛江师范学院 | Sepia lycidas fry breeding method |
| CN102939924A (en) * | 2012-11-13 | 2013-02-27 | 宁波大学 | Method for artificially breeding sepia lycidas gray |
| CN103843972A (en) * | 2014-02-28 | 2014-06-11 | 广州优锐生物科技有限公司 | Feed additive, preparation method and application of feed additive |
| CN105111477A (en) * | 2015-09-29 | 2015-12-02 | 浙江工业大学 | Method for preparing antibacterial edible films |
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2018
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10136828A (en) * | 1996-11-13 | 1998-05-26 | Asahi Chem Ind Co Ltd | Fish bank and substrate therefor |
| CN102823529A (en) * | 2012-09-17 | 2012-12-19 | 湛江师范学院 | Sepia lycidas fry breeding method |
| CN102939924A (en) * | 2012-11-13 | 2013-02-27 | 宁波大学 | Method for artificially breeding sepia lycidas gray |
| CN103843972A (en) * | 2014-02-28 | 2014-06-11 | 广州优锐生物科技有限公司 | Feed additive, preparation method and application of feed additive |
| CN105111477A (en) * | 2015-09-29 | 2015-12-02 | 浙江工业大学 | Method for preparing antibacterial edible films |
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