CN103843722A - Pure breeding method for soft-shelled turtles - Google Patents
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Abstract
The invention discloses a pure breeding method for soft-shelled turtles. Soft-shelled turtle fries, soft-shelled turtles cultured in an outdoor storage pond, soft-shelled turtles cultured in a factory greenhouse and fertilized eggs of the soft-shelled turtles are purified, rejuvenated and selected for many times by multiple steps, and meanwhile, each culture condition is controlled; the soft-shelled turtles are multi-generationally bred and cyclically selected. According to the method, a temperature and periodical humidity combination control method is adopted for an artificial incubation process of the fertilized eggs, the purity of second-generation soft-shell turtle generations purely bred by the method reaches more than 98 percent, the purity of third-generation soft-shell turtle generations purely bred by the method is higher than 99.5 percent, and the shape similarity, the uniformity, the resilience, the growth speed and the yield per unit of soft-shelled turtle individuals are remarkably improved.
Description
Technical field
The present invention relates to the artificial breeding method of a kind of soft-shelled turtle, especially relate to a kind of method of soft-shelled turtle pedigree breeding.
Background technology
Hanshou soft-shelled turtle, has another name called Hanshou Shelled Turtle Trionyx Sinensis, Hanshou water fish etc.; belong to Reptilia; Chelonia, Trionychidae, soft-shelled turtle belongs to; because having significantly; being different from the morphological feature of other strain and originating in taking Hanshou is main Dongting Lake region, and its production traits and economic characters are more excellent, extensively introduced a fine variety, cultivate at home; china national geography sign protection product, and defined with specific a series of technical indicators.Compared with other soft-shelled turtle kinds, Hanshou soft-shelled turtle has that body thin slice is large, and shirt rim is wide and thick, and in abdomen, fat is yolk yellow; Physique stalwartness, creeps flexibly, and immunity is strong, fast growth, and survival rate is high.When edible, its meat is pure, delicate delicious, chews taste, and its nutritive value and medical value are also better than other populations.
But, the existing Hanshou soft-shelled turtle of the different groups to the relative closure comparison test (five groups × 100/group) of dividing into groups: throw seedling, go out greenhouse, turn the trainings such as the outer pool and carry out simultaneously, throw something and feed same brand, equivalent bait, and thrown something and fed by same keeper, and cultivate under the condition of culture of equal temperature, equal illumination, equal water quality management, when 24 monthly age, not there is bigger difference in the Hanshou soft-shelled turtle between on the same group: optimal set survival rate: the poorest composition motility rate=96%:82%; The individual counterpoise of optimal set: the poorest group of individual counterpoise=1050g:880g; Optimal set gross yield: the poorest group of gross yield=100.8Kg:2.16Kg.Its experimental result shows: soft-shelled turtle germplasm good and bad otherness in existing Hanshou is larger, and carrying out to purificate and rejuvenate is that the pedigree breeding work of Main Means is necessary.
CN 1895034 A disclose a kind of non-pollution and ecological large-scale soft-shelled turtle culturing method, comprise the following steps: the selection of breeding ecological environment; Preparation before putting in a suitable place to breed; Put in a suitable place to breed; The feed of throwing something and feeding; Disease prevention and cure; The present invention makes full use of natural conditions and the resource of pond periphery, from biology, ecology, environmentology mechanism, according to the biological property such as biologically in ecology occupy separately the feature of ecological niche and the life habit of soft-shelled turtle, feeding habits, grow, rationally utilize ecological balance cultural technique, build the best ecotope of soft-shelled turtle, and use the comprehensive diseases prevention of ecological method, to improve the growing environment of Shelled Turtle Trionyx Sinensis and to improve self resistance against diseases, but do not provide soft-shelled turtle purebred of seed selection advantage, and then improve the method for the quality of soft-shelled turtle.
CN 102669047 A disclose the nuisanceless ecological cultivation method of a kind of Shelled Turtle Trionyx Sinensis, it comprise arrange that pond, seed are put in a suitable place to breed, pool oxygenation, transplanting pasture and water, feedstuff feeding, daily management, disease control.In breeding environment and management, improve the growing environment of Shelled Turtle Trionyx Sinensis, thereby reach the quality of improving Shelled Turtle Trionyx Sinensis, but do not provide the method for improving Shelled Turtle Trionyx Sinensis quality by seed selection mode.
CN 101120662 A disclose a kind of method of seed selection improved variety of China turtle, by to first ecotope being selected, then successively close soft-shelled turtle, fertilized egg, young soft-shelled turtle, one-tenth soft-shelled turtle, standby parent soft-shelled turtle are selected, wherein the selection rate of close soft-shelled turtle is 80 ~ 85%, the selection rate of young soft-shelled turtle is 25 ~ 35%, becoming the selection rate of soft-shelled turtle is 45 ~ 55%, and the selection rate of standby parent soft-shelled turtle is 65 ~ 75%.Though the method has been carried out seed selection in each formative year of soft-shelled turtle, do not carry out many generations and breed seed selection, can not reach the object of pedigree breeding.
Xu Yinrong utilizes the distinctive proterties of Japanese soft-shelled turtle, with its ripe male soft-shelled turtle and the female soft-shelled turtle mating of ripe Shelled Turtle Trionyx Sinensis, the hybridization first filial generation of output has made up the shortcoming that Japanese soft-shelled turtle mouthfeel is poor, egg laying amount is less, and young soft-shelled turtle, young soft-shelled turtle premunition strengthen, and have greatly improved survival rate and marketing quality; The selection cross technical essential that it adopts is as follows: the Crossing system of good soft-shelled turtle kind is to take biparental cross (single crosses), and selecting pure Japanese soft-shelled turtle is that male parent, Shelled Turtle Trionyx Sinensis are for maternal.3～5 ages of male Japanese soft-shelled turtle, body weight at 2.0～3.0 kilograms, body surface without wound, neck neck without fishhook, build is round, shirt rim is generous, skin is glossy; 2～4 ages of female Shelled Turtle Trionyx Sinensis, body weight at 1～1.5 kilogram, build oval or circle, shirt rim is wider, skin is glossy.(Xu Yinrong; Good close soft-shelled turtle selection cross technology application [J]; Science is breeded fish; 02 phase in 2010).The method adopts selection cross technology to improve the quality of soft-shelled turtle, Shelled Turtle Trionyx Sinensis is carried out to pedigree breeding reaches the method for improving soft-shelled turtle quality but do not provide, and does not also carry out manyly for seed selection, can not ensure that the heritability of soft-shelled turtle of seed selection is stable.
At present, a large amount of research work has been done in cultivation and the seed selection to Shelled Turtle Trionyx Sinensis both at home and abroad, but the pedigree breeding work of not carrying out repeatedly, how purificating and rejuvenating from generation to generation is especially carried out pedigree breeding to specific soft-shelled turtle population (as Hanshou soft-shelled turtle); The problems such as existing Shelled Turtle Trionyx Sinensis selection still exists seed selection dynamics inadequate, and germplasm is degenerated, and Shelled Turtle Trionyx Sinensis interindividual variation is large, plesiomorphism degree is low, poor growth, resistance against diseases are weak and yield per unit area is low, or the seed selection cycle is long, and Financial cost is high.
Summary of the invention
The technical problem to be solved in the present invention is: solve germplasm degeneration in Shelled Turtle Trionyx Sinensis incubation in prior art, poor growth, a little less than premunition, the technical problems such as the seed selection cycle is long, and provide a kind of method of soft-shelled turtle pedigree breeding, shorten the cycle of soft-shelled turtle pedigree breeding, thereby by how to improve the purity of soft-shelled turtle population for pedigree breeding, dwindle the difference between soft-shelled turtle individuality.
The technical scheme that the present invention solves its technical problem employing is: a kind of method of soft-shelled turtle pedigree breeding, comprises the following steps:
Step 1: during 6 ~ August, go out to select the young seedling of the shell soft-shelled turtle of 3 ~ 5 days the healthy and strong body of the young seedling of purebred soft-shelled turtle from existing, put into outdoor storage pond, holding pond after then using 1.5 ~ 5.0% saline solution to soak to cultivate;
Step 2: cultivate the young seedling of soft-shelled turtle when going out shell 28 ~ 35 days in storage pond, holding pond, reject that doubtful assorted seedling or growth rate are slow, the not good individuality of build, and preferred individuality is gone to batch production greenhouse continue to be cultured to early June next year, control the air themperature in batch production greenhouse at 33 ~ 35 DEG C, water temperature is at 30 ~ 33 DEG C, the pH value of water is 7.5 ~ 8.5, and dissolved oxygen is greater than 30mg/L, and every day, daily ration, feeding quantity was 1.5 ~ 2% of soft-shelled turtle body weight;
Step 3: when outside air temperature raises, when outer pool water temperature can be stabilized in more than 28 DEG C, stop greenhouse heat supply, while making water temperature in batch production greenhouse drop to gradually 26 ~ 30 DEG C, the soft-shelled turtle of cultivating in harvesting batch production greenhouse also moves to the continuation cultivation of the outer pool, and after harvesting, reject indivedual doubtful hybrids, invalid body, and will reject lower than same sex average weight person by sex;
Step 4: the soft-shelled turtle is preferably that 4:1 ~ 6:1 puts into the outer pool as parent and cultivates with pond by female-male proportion, and joins from flock-mate, can produce fertilized egg when 3 age;
Step 5: will be that healthy fertilized egg is put into hatching house and carried out artificial incubation by detection in step 4;
Step 6: by purebred the soft-shelled turtle obtaining in step 5 second filial by step 1 ~ tetra-method again purificate and rejuvenate, cultivating and managing, when 3 age, can again obtain fertilized egg, again the fertilized egg obtaining is obtained to healthy and strong young seedling, i.e. soft-shelled turtle F3 by the method hatching, cultivating of step 5.
Further, in step 1, the healthy and strong body of the young seedling of purebred soft-shelled turtle is base plate pale red, more connects the young seedling of rotund soft-shelled turtle without black splotch, build compared with other strain.
Further, in step 5, detect for healthy fertilized egg be chorion glossy, show slightly pink and 24 ~ 30 hours animal pole white dots of output can extend to oophyte half and the clear clearly demarcated fertilized egg of outline line.
Further, in step 1, outdoor storage pond, holding pond is 10 ~ 12m
3cement pit, if blowdown apparatus, put seedling and train water with large grass in first 7 days, and with lime disinfection, water quality requirement girth of a garment appropriateness, transparency 20 ~ 25cm, water temperature is in 28 ~ 35 DEG C, the pH value of water is 6.5 ~ 8.0, in storage pond, holding pond, put into and can cover the weter hyacinth of 30 ~ 50% waters surface or spray sward and perch for young seedling and hide strong illumination, outdoor storage pond, holding pond is being thrown the first 24 hours EM bacterium of splashing of seedling, and the young seedling of soft-shelled turtle that outdoor storage pond, holding pond is cultivated is going out shell after 7 ~ 8 days, the young soft-shelled turtle material of full price that the crude protein content of throwing something and feeding is 48 ~ 50%.
Further, in step 2, batch production hot-house culture condition is: batch production greenhouse comprises the installations and facilities for temperature adjustment, exhausting, oxygenation, water filling, blowdown, every day, at least blowdown was once, and more renew at any time water according to pond water degree of aging, also can use active ferment or photosynthetic bacteria and carry out biological cleaning; Matter, quantitative principle are adhered to regularly, located, determine to prevention from suffering from the diseases, bait feeding.
Preferably, in step 4, female-male proportion is generally 4:1 in primiparity year, and next year is 5:1, the 3rd year and be 6:1 later.
Further, in step 5, in artificial incubation process hatching medium, temperature is controlled at 26 ~ 34 DEG C, preferably 31.5 ~ 33.5 DEG C.
Further, in step 5, the humidity of artificial incubation process hatching house adopts interim humidity control method, and within 1st ~ 2 weeks, humidity is controlled at 80 ~ 82%, and within 3rd ~ 4 weeks, humidity is controlled at 82 ~ 84%, and the 5th week ~ humidity of emerging is controlled at 84 ~ 86%; When accumulated temperature reaches 34000 ~ 36000 DEG C, can obtain the most healthy and the strongest young seedling, i.e. the purebred second filial of soft-shelled turtle.
Further, described soft-shelled turtle is the Hanshou soft-shelled turtle of lakeside, Chinese Dongting Lake.
The beneficial effect of the method for a kind of soft-shelled turtle pedigree breeding of the present invention:
1) select to purificate and rejuvenate with the young seedling of existing soft-shelled turtle, the soft-shelled turtle in young seedling stage is easily differentiated, has improved the precision that pedigree breeding is selected, and has reduced the cultivation time of parental generation P1;
2) pedigree breeding adopts batch production hot-house culture early stage, makes the nurturing period in 4 years/generation of prior art foreshorten to 3 years/generation, has significantly shortened the cycle of soft-shelled turtle pedigree breeding;
3) fertilized egg artificial incubation process adopts the control method that temperature and interim humidity combine, and has improved the incubation rate of fertilized egg, has improved the quality of soft-shelled turtle in soft-shelled turtle pedigree breeding process;
4) purity of carrying out by the method the soft-shelled turtle second filial population that pedigree breeding obtains reaches more than 98%, and the purity of soft-shelled turtle F3 population is higher than 99.5%;
5) soft-shelled turtle pedigree breeding can improve plesiomorphism degree, regularity, anti-adversity ability and growth rate between soft-shelled turtle individuality, the yield per unit area of soft-shelled turtle.
Brief description of the drawings
Fig. 1 is the method flow diagram of a kind of soft-shelled turtle pedigree breeding of the present invention.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
With reference to Fig. 1:
embodiment 1:
Step 1: July, to go out to select the shell young seedling of Hanshou soft-shelled turtle of 4 days base plate be pale red and more connect the healthy and strong body of the young seedling of rotund purebred Hanshou soft-shelled turtle without black splotch, build compared with other strain from existing, the young seedling of Hanshou soft-shelled turtle is put into outdoor storage pond, holding pond with 1.5% saline solution immersion after 5 minutes and is cultivated, and outdoor storage pond, holding pond is 10m
3cement pit, if blowdown apparatus, is highly 60cm, water filling 40cm, put seedling and train water with large grass in first 7 days, and with lime disinfection, water quality requirement girth of a garment appropriateness, transparency 20cm, water temperature is in 28 ~ 32 DEG C, the pH value of water is 6.5 ~ 7.0, puts into can cover the weter hyacinth of 30 ~ 40% waters surface or spray sward perches for young seedling and hides strong illumination in storage pond, holding pond, and outdoor storage pond, holding pond is being thrown the first 24 hours EM bacterium of splashing of seedling; Go out shell yolk nutrition on the 7th at young seedling and be absorbed completely, after digestive system function is sound, the young soft-shelled turtle material of the full price of can throwing something and feeding, its main component is white fish meal 63%, A starch 18%, fermented bean dregs 11.3%, tanimoto powder 4%, calcium dihydrogen phosphate 2%, premix 1%;
Step 2: cultivate the young seedling of Hanshou soft-shelled turtle to going out shell 28 ~ 30 in storage pond, holding pond, when interindividual variation is obvious, reject that doubtful assorted seedling or growth rate are slow, the not good individuality of build, and preferred individuality is gone to batch production greenhouse continue to be cultured to early June next year, air themperature in control batch production greenhouse is at 33 ~ 35 DEG C, water temperature is at 30 ~ 33 DEG C, the pH value of water is 7.5 ~ 8.5, dissolved oxygen is 50mg/L, twice of blowdown every day, and more renew at any time water according to pond water degree of aging, and use a small amount of active ferment or photosynthetic bacteria carries out biological cleaning.Matter, quantitative principle are adhered to regularly, located, determine to prevention from suffering from the diseases, bait feeding, and every day, daily ration, feeding quantity was 1.5% of Hanshou soft-shelled turtle body weight;
Step 3: when outside air temperature raises, when outer pool water temperature can be stabilized in more than 28 DEG C, stop greenhouse heat supply, while making water temperature in batch production greenhouse drop to gradually 26 ~ 30 DEG C, the soft-shelled turtle of cultivating in harvesting batch production greenhouse also moves to the continuation cultivation of the outer pool, and outer pool water temperature is generally stabilized in 26 ~ 28 DEG C; And after harvesting, reject indivedual doubtful hybrids, invalid body, and will reject lower than same sex average weight person by sex;
Step 4: the Hanshou soft-shelled turtle is preferably that 5:1 puts into the outer pool as parent and cultivates with pond by female-male proportion, and joins from flock-mate, can produce fertilized egg when 3 age;
Step 5: by glossy chorion in step 4, show slightly 24 ~ 30 hours animal pole white dots of pink and output and can extend to oophyte half and the clear clearly demarcated fertilized egg of outline line and put into hatching house and carry out artificial incubation, in hatching medium, temperature is controlled at 32.5 ± 0.5 DEG C; The humidity of hatching house adopts interim humidity control method, and 1st ~ 2 weeks humidity is controlled at 81%, the 3 ~ 4 week humidity and is controlled at 83%, the 5 week ~ humidity of emerging and is controlled at 86%; When accumulated temperature reaches 35000 DEG C, obtain the most healthy and the strongest young seedling, i.e. the purebred second filial of Hanshou soft-shelled turtle;
Step 6: by the purebred second filial of Hanshou soft-shelled turtle obtaining in step 5 by step 1 ~ tetra-method again purificate and rejuvenate, cultivating and managing, when 3 age, can again obtain fertilized egg, again the fertilized egg obtaining is obtained to healthy and strong young seedling by the method hatching, cultivating of step 5, form product lover, high-output stress-resistance, fast growth, regularity is good, specific yield is high and the Hanshou soft-shelled turtle F3 of performance energy genetic stability.
Carry out pedigree breeding by said method, the purity of the second filial population of Hanshou soft-shelled turtle reaches 98.5%, and the purity of the F3 population of Hanshou soft-shelled turtle is 99.7%.
embodiment 2:
Step 1: August, to go out to select the shell young seedling of Hanshou soft-shelled turtle of 5 days base plate be pale red and more connect the healthy and strong body of the young seedling of rotund purebred Hanshou soft-shelled turtle without black splotch, build compared with other strain from existing, the young seedling of Hanshou soft-shelled turtle is put into outdoor storage pond, holding pond with 1.5% saline solution immersion after 4 minutes and is cultivated, and outdoor storage pond, holding pond is 12m
3cement pit, if blowdown apparatus, is highly 60cm, water filling 40cm, put seedling and train water with large grass in first 7 days, and with lime disinfection, water quality requirement girth of a garment appropriateness, transparency 25cm, water temperature is in 32 ~ 35 DEG C, the pH value of water is 7.0 ~ 8.0, puts into can cover the weter hyacinth of 40 ~ 50% waters surface or spray sward perches for young seedling and hides strong illumination in storage pond, holding pond, and outdoor storage pond, holding pond is being thrown the first 24 hours EM bacterium of splashing of seedling; Go out shell yolk nutrition on the 8th at young seedling and be absorbed completely, after digestive system function is sound, the young soft-shelled turtle material of the full price of can throwing something and feeding, its main component is white fish meal 63%, A starch 18%, fermented bean dregs 11.3%, tanimoto powder 4%, calcium dihydrogen phosphate 2%, premix 1%;
Step 2: cultivate the young seedling of Hanshou soft-shelled turtle to going out shell 32 ~ 35 in storage pond, holding pond, when interindividual variation is obvious, reject again the individuality that doubtful assorted seedling or growth rate are slow, build is not good and preferred individuality is gone to batch production greenhouse and continue to be cultured to early June next year, air themperature in control batch production greenhouse is at 33 ~ 35 DEG C, water temperature is at 30 ~ 33 DEG C, the pH value of water is 7.5 ~ 8.5, dissolved oxygen is 50mg/L, twice of blowdown every day, and more renew at any time water according to pond water degree of aging, and use a small amount of active ferment or photosynthetic bacteria carries out biological cleaning.Matter, quantitative principle are adhered to regularly, located, determine to prevention from suffering from the diseases, bait feeding, and every day, daily ration, feeding quantity was 2.0% of Hanshou soft-shelled turtle body weight;
Step 3: when outside air temperature raises, when outer pool water temperature can be stabilized in more than 28 DEG C, stop greenhouse heat supply, while making water temperature in batch production greenhouse drop to gradually 26 ~ 30 DEG C, the soft-shelled turtle of cultivating in harvesting batch production greenhouse also moves to the continuation cultivation of the outer pool, and outer pool water temperature is generally stabilized in 29 ~ 30 DEG C; And after harvesting, reject indivedual doubtful hybrids, invalid body, and will reject lower than same sex average weight person by sex;
Step 4: the Hanshou soft-shelled turtle is preferably that 4:1 puts into the outer pool as parent and cultivates with pond by female-male proportion, and joins from flock-mate, can produce fertilized egg when 3 age;
Step 5: by glossy chorion in step 4, show slightly 24 ~ 30 hours animal pole white dots of pink and output and can extend to oophyte half and the clear clearly demarcated fertilized egg of outline line and put into hatching house and carry out artificial incubation, in hatching medium, temperature is controlled at 30 ± 0.5 DEG C; The humidity of hatching house adopts interim humidity control method, and 1st ~ 2 weeks humidity is controlled at 82%, the 3 ~ 4 week humidity and is controlled at 84%, the 5 week ~ humidity of emerging and is controlled at 85%; When accumulated temperature reaches 36000 DEG C, obtain the most healthy and the strongest young seedling, i.e. the purebred second filial of Hanshou soft-shelled turtle;
Step 6: by the purebred second filial of Hanshou soft-shelled turtle obtaining in step 5 by step 1 ~ tetra-method again purificate and rejuvenate, cultivating and managing, when 3 age, can again obtain fertilized egg, again the fertilized egg obtaining is obtained to healthy and strong young seedling by the method hatching, cultivating of step 5, form product lover, high-output stress-resistance, fast growth, regularity is good, specific yield is high and the Hanshou soft-shelled turtle F3 of performance energy genetic stability.
Carry out pedigree breeding by said method, the purity of the second filial population of Hanshou soft-shelled turtle reaches 98.2%, and the purity of the F3 population of Hanshou soft-shelled turtle is 99.6%.
Claims (9)
1. a method for soft-shelled turtle pedigree breeding, is characterized in that, comprises the following steps:
Step 1: during 6 ~ August, go out to select the young seedling of the shell soft-shelled turtle of 3 ~ 5 days the healthy and strong body of the young seedling of purebred soft-shelled turtle from existing, put into outdoor storage pond, holding pond after then using 1.5 ~ 5.0% saline solution to soak to cultivate;
Step 2: cultivate the young seedling of soft-shelled turtle when going out shell 28 ~ 35 days in storage pond, holding pond, reject that doubtful assorted seedling or growth rate are slow, the not good individuality of build, and preferred individuality is gone to batch production greenhouse continue to be cultured to early June next year, control the air themperature in batch production greenhouse at 33 ~ 35 DEG C, water temperature is at 30 ~ 33 DEG C, the pH value of water is 7.5 ~ 8.5, and dissolved oxygen is greater than 30mg/L, and every day, daily ration, feeding quantity was 1.5 ~ 2% of soft-shelled turtle body weight;
Step 3: when outside air temperature raises, when outer pool water temperature can be stabilized in more than 28 DEG C, stop greenhouse heat supply, while making water temperature in batch production greenhouse drop to gradually 26 ~ 30 DEG C, the soft-shelled turtle of cultivating in harvesting batch production greenhouse also moves to the continuation cultivation of the outer pool, and after harvesting, reject indivedual doubtful hybrids, invalid body, and will reject lower than same sex average weight person by sex;
Step 4: the soft-shelled turtle is preferably that 4:1 ~ 6:1 puts into the outer pool as parent and cultivates with pond by female-male proportion, and joins from flock-mate, can produce fertilized egg when 3 age;
Step 5: will be that healthy fertilized egg is put into hatching house and carried out artificial incubation by detection in step 4;
Step 6: by purebred the soft-shelled turtle obtaining in step 5 second filial by step 1 ~ tetra-method again purificate and rejuvenate, cultivating and managing, when 3 age, can again obtain fertilized egg, again the fertilized egg obtaining is obtained to healthy and strong young seedling, i.e. soft-shelled turtle F3 by the method hatching, cultivating of step 5.
2. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, is characterized in that, the healthy and strong body of the young seedling of purebred soft-shelled turtle described in step 1 is base plate pale red, more connects the young seedling of rotund soft-shelled turtle without black splotch, build compared with other strain.
3. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, it is characterized in that, described in step 5, detect for healthy fertilized egg be chorion glossy, show slightly pink and 24 ~ 30 hours animal pole white dots of output can extend to oophyte half and the clear clearly demarcated fertilized egg of outline line.
4. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, is characterized in that, outdoor storage pond, holding pond described in step 1 is 10 ~ 12m
3cement pit, if blowdown apparatus, put seedling and train water with large grass in first 7 days, and with lime disinfection, water quality requirement girth of a garment appropriateness, transparency 20 ~ 25cm, water temperature is in 28 ~ 35 DEG C, the pH value of water is 6.5 ~ 8.0, in storage pond, holding pond, put into and can cover the weter hyacinth of 30 ~ 50% waters surface or spray sward and perch for young seedling and hide strong illumination, outdoor storage pond, holding pond is being thrown the first 24 hours EM bacterium of splashing of seedling, and the young seedling of soft-shelled turtle that outdoor storage pond, holding pond is cultivated is going out shell after 7 ~ 8 days, the young soft-shelled turtle material of full price that the crude protein content of throwing something and feeding is 48 ~ 50%.
5. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, it is characterized in that, the hot-house culture of batch production described in step 2 condition is: batch production greenhouse comprises the installations and facilities for temperature adjustment, exhausting, oxygenation, water filling, blowdown, every day, at least blowdown was once, and more renew at any time water according to pond water degree of aging, also can use active ferment or photosynthetic bacteria and carry out biological cleaning; Matter, quantitative principle are adhered to regularly, located, determine to prevention from suffering from the diseases, bait feeding.
6. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, is characterized in that, female-male proportion described in step 4 is generally 4:1 in primiparity year, and next year is 5:1, the 3rd year and be 6:1 later.
7. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, is characterized in that, in the process of artificial incubation described in step 5 hatching medium, temperature is controlled at 26 ~ 34 DEG C, preferably 31.5 ~ 33.5 DEG C.
8. a kind of method of soft-shelled turtle pedigree breeding as claimed in claim 1, it is characterized in that, the humidity of the process of artificial incubation described in step 5 hatching house adopts interim humidity control method, within 1st ~ 2 weeks, humidity is controlled at 80 ~ 82%, within 3rd ~ 4 weeks, humidity is controlled at 82 ~ 84%, and the 5th week ~ humidity of emerging is controlled at 84 ~ 86%; When accumulated temperature reaches 34000 ~ 36000 DEG C, can obtain the most healthy and the strongest young seedling, i.e. the purebred second filial of soft-shelled turtle.
9. a kind of method of soft-shelled turtle pedigree breeding as described in one of claim 1 ~ 8, is characterized in that, described soft-shelled turtle is the Hanshou soft-shelled turtle of lakeside, Chinese Dongting Lake.
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| CN104115784A (en) * | 2014-07-09 | 2014-10-29 | 绍兴中亚工贸园有限公司 | Fast soft-shelled turtle breeding technology |
| CN104381159A (en) * | 2014-09-30 | 2015-03-04 | 湖北宏旺生态农业科技有限公司 | Cultivation and breeding method of improved-variety Chinese soft-shelled turtles |
| CN105230556A (en) * | 2014-07-07 | 2016-01-13 | 龚老汉控股集团有限公司 | Whole-cource pollution-free and ecological cultivation method for Chinese softshell turtles |
| CN107691352A (en) * | 2017-09-30 | 2018-02-16 | 楚雄彝族自治州种猪种鸡场 | Boar hybridizing method |
| CN114208769A (en) * | 2021-12-16 | 2022-03-22 | 湖南省河洲生态甲鱼养殖有限公司 | Soft-shelled turtle physique training device and training method |
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| CN102669047A (en) * | 2012-04-25 | 2012-09-19 | 李进村 | Nuisanceless ecological culturing method for Chinese soft shell turtle |
| CN102696537A (en) * | 2012-05-14 | 2012-10-03 | 安徽省喜佳农业发展有限公司 | Greenhouse cultivation technology of soft-shelled turtles |
| CN102972346A (en) * | 2012-12-13 | 2013-03-20 | 梁贺 | Culture method based on graded stocking and cyclic utilization of soft-shelled turtles in greenhouse |
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2013
- 2013-08-07 CN CN201310340156.XA patent/CN103843722B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895034A (en) * | 2005-07-14 | 2007-01-17 | 杨月兰 | Non-pollution and ecological large-scale soft-shelled turtle culturing method |
| CN101120662A (en) * | 2007-08-04 | 2008-02-13 | 杭州萧山天福生物科技有限公司 | Method for selectively breeding improved variety of China turtle |
| CN102669047A (en) * | 2012-04-25 | 2012-09-19 | 李进村 | Nuisanceless ecological culturing method for Chinese soft shell turtle |
| CN102696537A (en) * | 2012-05-14 | 2012-10-03 | 安徽省喜佳农业发展有限公司 | Greenhouse cultivation technology of soft-shelled turtles |
| CN102972346A (en) * | 2012-12-13 | 2013-03-20 | 梁贺 | Culture method based on graded stocking and cyclic utilization of soft-shelled turtles in greenhouse |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230556A (en) * | 2014-07-07 | 2016-01-13 | 龚老汉控股集团有限公司 | Whole-cource pollution-free and ecological cultivation method for Chinese softshell turtles |
| CN104115784A (en) * | 2014-07-09 | 2014-10-29 | 绍兴中亚工贸园有限公司 | Fast soft-shelled turtle breeding technology |
| CN104381159A (en) * | 2014-09-30 | 2015-03-04 | 湖北宏旺生态农业科技有限公司 | Cultivation and breeding method of improved-variety Chinese soft-shelled turtles |
| CN104381159B (en) * | 2014-09-30 | 2022-12-20 | 湖北宏旺生态农业科技股份有限公司 | Fine breed culture and breeding method for Chinese softshell turtles |
| CN107691352A (en) * | 2017-09-30 | 2018-02-16 | 楚雄彝族自治州种猪种鸡场 | Boar hybridizing method |
| CN114208769A (en) * | 2021-12-16 | 2022-03-22 | 湖南省河洲生态甲鱼养殖有限公司 | Soft-shelled turtle physique training device and training method |
| CN114208769B (en) * | 2021-12-16 | 2022-11-29 | 湖南省河洲生态甲鱼养殖有限公司 | Soft-shelled turtle physique training device and training method |
| CN114793988A (en) * | 2022-04-19 | 2022-07-29 | 中国科学院海洋研究所 | Method for identifying ray porosa fertilized eggs based on morphology |
| CN114793988B (en) * | 2022-04-19 | 2023-01-17 | 中国科学院海洋研究所 | Morphology-based method for identifying fertilized eggs of raja porosa |
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