CN104957064B - A kind of method for culturing seedlings of tiger spot cuttlefish - Google Patents
A kind of method for culturing seedlings of tiger spot cuttlefish Download PDFInfo
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- CN104957064B CN104957064B CN201510386878.8A CN201510386878A CN104957064B CN 104957064 B CN104957064 B CN 104957064B CN 201510386878 A CN201510386878 A CN 201510386878A CN 104957064 B CN104957064 B CN 104957064B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of method for culturing seedlings of tiger spot cuttlefish, including：Tiger spot sea grape is framed up, and is fixed on hatching in the water away from the 30cm of bottom of pond 20；Accurately regulation and control water Inversion phenomenon and pH value, makes up to optimal hatching and nursery state；Take optimum tiger spot hatching cuttlefish and the environment of nursery；Scheme is hatched using shallow water, and simulate tidal bulge lakie in nature change water method, efficiently management water；The tiger spot cuttlefish nursery daily management operational procedure of setting science.Production cost can be greatly reduced in the present invention, improve the hatchability and survival rate of tiger spot cuttlefish nursery, cultivate the seedling of more high-quality.
Description
Technical field
It is a kind of method for culturing seedlings of tiger spot cuttlefish the invention belongs to aquaculture category.
Background technology
Tiger spot cuttlefish, is commonly called as inkfish, is the higher seawater fish of economic value.Because the tiger spot cuttlefish resource in ocean is tight
Weight is exhausted, market supply shortage, and people suddenly treat to cultivate tiger spot cuttlefish to fill up the vacancy that market is huge by workman.However, brave
The factor such as spot cuttlefish nursery hatchability is low, seedling is uneven, survival rate is low, quantity of exchanged water is big, causes seedling cost too high, body is long
3cm cuttlefish seedling price is up to 6 yuan/tail, seriously constrains the large-scale promotion cultivation of this famous-brand and high-quality kind, how conscientiously may be used
Handle the urgent problem to be solved such as above-mentioned well capablely, to reduce production cost, seedling quality is improved, to promoting tiger spot cuttlefish industry
Development be significant.
The content of the invention
The inventors found that the hatching and its growth of the young of the natural environment optimum tiger spot sea grape at the South Sea,
Variation in water pressure caused by tiger spot inkfish spawning position, the intrinsic tide bulge and fall of illuminance and sea area of hatching demand etc., be all
The very long evolution of tiger spot cuttlefish, the result of reform of nature environment.According to the life habit of tiger spot cuttlefish, corresponding nursery ring is simulated
Border, correlates the custom that the ambuscade of tiger spot cuttlefish is ingested, carries out hatching, cultivating, can greatly improve the hatchability and survival rate of seedling.
The present invention designs and develops a kind of innovative approach of tiger spot cuttlefish nursery, allows tiger spot sea grape energy close to the environment in seabed
Hatching, and have the change of similar flood tide ebb twice on the 1st, can be effectively reduced production cost, can improve nursery hatchability and into
Motility rate, make the fry that hatches evenly, it is vibrant.
The technical scheme that the present invention is provided is：
A kind of method for culturing seedlings of tiger spot cuttlefish, including：
Step 1, selected pond depth 1.2-1.5m, each cement pool area about 15m2, choose frame height 15-20cm, dark, band
The PVC plastic material frame dress ovum of lid is hatched.
Step 2, elder generation lay oxygen increasing tube at hatchery bottom, and are connected to aerator, further according to the length of PVC plastic material frame, use
Frame of plastic is fixed on away from bottom of pond 20-30cm by stainless steel frame or brick, then the gauge on cement pool wall subscript, with plastics
Frame top horizontal plane is 0, and 50cm places are+50 on frame top, then on oxygen increasing tube, under the horizontal line of frame of plastic top, are hung
Intelligence electrical bar, every 5 square metres hang 1 2kW electrical bar.
Step 3, when taking ovum, to cut off based on the attachment bases such as marine alga, palm rope, do not damage as far as possible and twine ovum root, will be into ovum
String or ovum grain are put into PVC plastic material frame, and every square decimeter about puts 30, closes the lid and fastens.
Step 4, hatching water quality requirement pH value 7.4 ± 0.2, salinity 27.5-28.5 ‰, 25-26 DEG C of water temperature, water dissolved oxygen ＞
7.5 mg/litres, ammonia-nitrogen content ＜ 0.1mg/L, content of nitrite ＜ 0.05mg/L.Intake to before nursery pond, first measure every
Index, ammonia nitrogen, nitrite it is not up to standard to change water, water dissolved oxygen is solved by oxygenation, and pH value can be heightened with quick lime, use vinegar
Acid or lactic acid bacteria microbe formulation are turned down；Salinity can be heightened with bittern, be turned down with fresh water；It can be added with the electrical bar of intelligence to water body
Temperature, can use sealed plastic bag ice block cooling, and water for larval nursing will can be used through active carbon purifying rear, enter water logging and cross frame of plastic
Push up 0-50cm hatchings.
Step 5, using shallow water hatch scheme, to reduce cost.The frame of plastic top equipped with fish-egg had just been flooded using water as 0
It is+50 at 50cm on position, frame top, 6h of first at the uniform velocity intaking daily makes water level rise to+50 from 0, then at the uniform velocity draining 6h, makes water
Position is down to 0 from+50；At the uniform velocity intake again 6h, water level is risen to+50 again, then at the uniform velocity draining 6h again, water level is returned back to 0 again,
Go round and begin again, allow fish-egg to experience the variation in water pressure of similar tidal bulge lakie, and occur high tide level low tide twice twice in 1d.
Step 6, hatching and nurturing period, to be continuously oxygenated, the illumination of nursery workshop ＜ 150lux, noise ＜ 65db.
Step 7, the developmental state for checking fish-egg daily, inhale bottom to pond every 3d and remove contamination 1 time；Started at from Gunther parent fish spawning,
Hatch to 17d, start frame lid, depauperation or dead egg white are gone out into nursery pond, to prevent nursery water quality from ruining.
Step 8, the hatching through 20d, substantial amounts of cuttlefish seedling break tire successively and gone out, single body weight about 0.2g, according to about 400
Only/m2Density plan next step cultivation.Point pond method is to estimate the fry amount hatched in this hatchery to reach accordingly
After cultivation density, water hose frame is connected with the basin more slightly larger than frame of plastic the fish-egg do not hatched is moved to the continuation hatching of another hatchery,
Such one-level level ground point pond, until hatching is finished.
Step 9, squid larva nurturing period, bait is fed twice daily in morning and evening, with isometric with squid larva trunk width
Shrimp seedling or artemia live body, as the bait of squid larva, each daily ration, feeding quantity to make the living body bait of water body reach 2-3 only/L,
Equivalent to the 4-5% that every daily ration of feeding is squid larva body weight.
During step 10, larval culture, water quality requirement can relax, 19-28 DEG C of water temperature, salinity 23-30 ‰, pH value 6.8-
7.8；Pond suction bottom, which is removed contamination, to be changed to 1 time every other day, and it is identical with during hatching that other change water method, environmental requirement etc..By three weeks
Cultivate, cuttlefish seedling trunk length can be sold with regard to that can reach more than 2cm as commercial seedling.
Preferably, in the method for culturing seedlings of described tiger spot cuttlefish, in the step 2, sea grape is placed on away from bottom of pond
Hatch in 20-30cm water.
Preferably, in the method for culturing seedlings of described tiger spot cuttlefish, in the step 5, scheme is hatched using shallow water, and
Tidal bulge lakie changes water method in natural imitation circle.
Preferably, in the method for culturing seedlings of described tiger spot cuttlefish, in the step 9, with wide about with squid larva trunk
The isometric shrimp seedling of degree or artemia live body, as the open-mouthed bait of squid larva, are counted with the 2-3/L of density of living body bait in water body
Measure each daily ration, feeding quantity.
Preferably, in the method for culturing seedlings of described tiger spot cuttlefish, in the step 4, vinegar is heightened or used using quick lime
Acid, lactic acid bacteria microbe formulation turn down pH value, make up to 7.4 ± 0.2；Heightened using bittern or turn down salinity with fresh water, made
Reach 27.5-28.5 ‰；Using the electrical bar heating of intelligence or with sealed plastic bag ice block cooling, reach water temperature
25-26℃。
Preferably, in the method for culturing seedlings of described tiger spot cuttlefish, in the step 6, with the Luxs of workshop illumination ＜ 150
It is used as seedling raising environment.
Preferably, the time for feeding bait in the step 9 twice sooner or later is respectively 05：00、19：00.
The present invention devises a kind of method for culturing seedlings of tiger spot cuttlefish.Firstth, the present invention uses and sea grape is placed on away from pond
At the 20-30cm of bottom, the environment of tiger spot inkfish spawning is hatched in simulation nature；Secondth, the present invention is using shallow water hatching side
Tidal bulge lakie changes water method in case, and natural imitation circle；3rd, the present invention is using about isometric with squid larva trunk width
Shrimp seedling living or artemia live body, as the open-mouthed bait of squid larva, with the 2-3/L of density of living body bait in water body as each
Daily ration, feeding quantity；4th, the present invention is heightened using quick lime or is turned down pH value with acetic acid, lactic acid bacteria microbe formulation, is adjusted using bittern
Height turns down salinity with fresh water, using the electrical bar heating of intelligence or with sealed plastic bag ice block cooling, regulates and controls nursery water
Matter；5th, the present invention is used as seedling raising environment using workshop illumination ＜ 150lux.
Brief description of the drawings
Fig. 1 is nursery flow chart
Fig. 2 is nursery pond artwork
Fig. 3 is that cuttlefish seedling hatches sketch
Embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to specification text
Word can be implemented according to this.
As illustrated, the present invention provides a kind of embodiment of tiger spot cuttlefish nursery, including：
Step 1, selected 1.2-1.5 meters of pond depth, each cement pool area about 15m2, cement pit be preferably it is semi-buried, i.e.,
One half pond body is embedded in underground, such available buffer temperature mutation；Choose frame height 15-20cm, dark, with cover PVC plastic material frame is filled
Ovum is hatched, the length and width size of frame of plastic, is advisable in order to operate.
Step 2, elder generation lay oxygen increasing tube at nursery pond bottom, and are connected to aerator, further according to the length of PVC plastic material frame, use
Frame of plastic is fixed on away from bottom of pond 20-30cm by stainless steel frame or brick, and the ornaments width of stainless steel frame or brick is than PVC plastic material frame
Length is slightly smaller to be advisable, then the gauge on cement pool wall subscript, using frame of plastic top horizontal plane as 0, be at 50cm on frame top+
50, then on oxygen increasing tube, under the horizontal line of frame of plastic top, the electrical bar of intelligence is hung, in every 5m2Hang 1 2kW electricity
Hot pin, electrical bar hanging position is critically important, because electrical bar is hung on oxygen increasing tube, can most make the quick even heat of water temperature, by electricity
Hot pin is hung under the horizontal line of frame of plastic top, the electrical bar moment is maintained in water and is not to burn out.
For example：4m × 4m × 1.2m cement pit, the frame of plastic length chosen are 50cm × 40cm, and oxygen increasing tube is laid on bottom of pond
Center, oxygen increasing tube the right and left puts stainless steel frame or brick, can put 3 row per side, each column can put 6-8 frame of plastic, stainless steel frame
Width should make 30-40cm or that brick is put into 30-40cm is wide, frame of plastic is disposed across above stainless steel frame or brick, and with thin
Rope system is fixed.
Step 3, when taking ovum, to cut off based on the attachment bases such as marine alga, palm rope, do not damage as far as possible and twine ovum root, will be into ovum
String or ovum grain are gently put into PVC plastic material frame, and every square decimeter about puts 30, closes the lid and fastens, to prevent ovum string or ovum grain
Drift out.
Step 4, hatching water quality requirement pH value 7.4 ± 0.2, salinity 27.5-28.5 ‰, 25-26 DEG C of water temperature, water dissolved oxygen ＞
7.5 mg/litres, ammonia-nitrogen content ＜ 0.1mg/L, content of nitrite ＜ 0.05mg/L.Intake to before nursery pond, not only to pass through
Active carbon purifying processing, and first measure indices, ammonia nitrogen, nitrite it is not up to standard to change water, water dissolved oxygen can pass through
Oxygenation is solved, and pH value can be heightened with quick lime, turned down with acetic acid or lactic acid bacteria microbe formulation；Salinity can be heightened with bittern, be used
Fresh water is turned down；The electrical bar heating of water temperature intelligence, can use sealed plastic bag ice block cooling, enter water logging and cross frame of plastic top 0-
50cm is hatched.
For example：Heated with the electrical bar of intelligence, set water temperature less than 25 DEG C during hatching plus electrified regulation, higher than 25.5 DEG C
Power-off；Less than 19 DEG C electrified regulations of water temperature, higher than 23 DEG C power-off are then set during larval culture.
Step 5, using shallow water hatch scheme, to reduce cost.The frame of plastic top equipped with fish-egg had just been flooded using water as 0
It is+50 at 50cm on position, frame top, 6h of first at the uniform velocity intaking daily makes water level rise to+50 from 0, then at the uniform velocity draining 6h, makes water
Position is down to 0 from+50；At the uniform velocity intake again 6h, water level is risen to+50 again, then at the uniform velocity draining 6h again, water level is returned back to 0 again,
Go round and begin again, allow fish-egg to experience the variation in water pressure of similar tidal bulge lakie, and occur high tide level low tide twice twice in 1d.Take
The difficult nursery of water, collects the water of nursery discharge, through the sterilization of the disinfectants such as chlorine dioxide, exposure, aeration, precipitation, filtering
Afterwards, recycled.
Step 6, hatching and nurturing period, to be continuously oxygenated, nursery workshop illumination ＜ 150lux, noise ＜ 65db, because
Cuttlefish seedling easily scaring tells ink, so should not cause high pitch during operation, tries not to use strong illumination cuttlefish seedling.
Step 7, the developmental state for checking fish-egg daily, it is found that fish-egg is excessively crowded, it is contemplated that point frame, mutually squeezed with reducing
Pressure；Bottom is inhaled every 3d to pond to remove contamination 1 time；Started at from Gunther parent fish spawning, hatch to 17d, start frame lid, by depauperation or dead
Egg white goes out nursery pond, to prevent nursery water quality from ruining.Cleaning work will be carried out in water, gently should not stave fish-egg.
Step 8, the hatching through 20d, substantial amounts of cuttlefish seedling break tire successively and gone out, single body weight about 0.2g, according to about 400
Only/m2Density plan next step cultivation.Point pond method is reached accordingly by estimating the fry amount hatched in this hatchery
Cultivation density after, untie for being fixed on frame of plastic on stainless steel frame or fragment of brick, connect water hose frame with the basin more slightly larger than frame of plastic
The fish-egg do not hatched is moved to another hatchery to continue to hatch, such one-level level ground point pond, until hatching is finished.
Step 9, squid larva nurturing period, daily in morning and evening (05：00、19：00) feed bait twice, with squid larva
The isometric shrimp seedling of trunk width or artemia live body, as the bait of squid larva, each daily ration, feeding quantity will make the living body bait of water body
2-3/L is reached, according to shrimp seedling or artemia this density calculating, equivalent to the 4-5% that every daily ration of feeding is body weight.This bait throwing in is set
Meter is the habit ingested of ambuscade for considering cuttlefish, and generally cuttlefish is to wait for prey and enters firing area, and initiation suddenly is attacked
Hit, its firing area is 2-3 times from body length.
During step 10, larval culture, squid larva adaptability enhancing, water quality requirement can suitably relax, water temperature 19-28
DEG C, salinity 23-30 ‰, pH value 6.8-7.8；Pond inhales bottom and removes contamination and is changed to 1 time every other day, other change water method, environmental requirement etc. with
It is identical during hatching.By the cultivation of three weeks, cuttlefish seedling trunk length can be sold with regard to that can reach more than 2cm as commercial seedling.
Claims (3)
1. a kind of method for culturing seedlings of tiger spot cuttlefish, it is characterised in that including：
Step 1, selected nursery pond are cement pit, pond depth 1.2-1.5m, each nursery pool area 15m2, choose frame height 15-20cm,
Dark, with cover PVC plastic material frame dress ovum is hatched；
Step 2, first at nursery pond bottom oxygen increasing tube is laid, and be connected to aerator, further according to the length of PVC plastic material frame, with stainless
PVC plastic material frame is fixed on away from bottom of pond 20-30cm by steelframe or brick, then the gauge on nursery pool wall subscript, with PVC plastic
Material frame top horizontal plane is 0, and 50cm places are+50 on frame top, then on oxygen increasing tube, under the horizontal line of PVC plastic material frame top,
The electrical bar of intelligence is hung, per 5m2Hang 1 2kW electrical bar；
Step 3, by ovum string or ovum grain be put into PVC plastic material frame, every square decimeter puts 30, closes the lid and fastens；
Step 4, hatching water quality requirement pH value 7.4 ± 0.2, salinity 27.5-28.5 ‰, 25-26 DEG C of water temperature, water dissolved oxygen ＞ 7.5
Mg/litre, ammonia-nitrogen content ＜ 0.1mg/L, content of nitrite ＜ 0.05mg/L；Intake and water quality is measured to before nursery pond, ammonia nitrogen,
Nitrite it is not up to standard to change water, pH value quick lime or acetic acid, lactic acid bacteria microbe formulation regulation；Salinity bittern is light
Water is adjusted；Water temperature is heated with electrical bar or with sealed plastic bag ice block cooling, and water for larval nursing will be through active carbon purifying rear
It can be used；
Step 5, water inlet hatching, the PVC plastic material frame top equipped with fish-egg had just been flooded using water as 0,50cm on PVC plastic circle top
Locate as+50,6h of first at the uniform velocity intaking daily makes water level rise to+50 from 0, then at the uniform velocity draining 6h, makes water level be down to 0 from+50；
At the uniform velocity intake again 6h, water level is risen to+50 again, then at the uniform velocity draining 6h again, water level is returned back to 0 again, go round and begin again, allow fish
Ovum experiences the variation in water pressure of similar sea tidal bulge lakie；
Step 6, hatching and cultivating process, continuously be oxygenated, nursery workshop illuminance answers ＜ 150lux, noise ＜ 65db；
Step 7, the developmental state for checking fish-egg daily, inhale bottom to nursery pond every 3d and remove contamination 1 time；Start at, incubate from Gunther parent fish spawning
Change to 17d, start frame lid, depauperation or dead egg white are gone out into nursery pond, to prevent nursery water quality from ruining；
Step 8, the hatching through 20d, substantial amounts of cuttlefish seedling break tire successively and gone out, according to 400/m2Density planning next step
Cultivate；Point pond method of seedling is to estimate the fry amount hatched in this nursery pond to reach after corresponding cultivation density, with than PVC
The slightly larger basin of frame of plastic connects water hose frame and the fish-egg do not hatched is moved to the continuation hatching of another nursery pond；
Step 9, squid larva nurturing period, bait is fed twice daily in morning and evening, with the shrimp isometric with squid larva trunk width
Seedling or artemia live body, as the bait of squid larva, each daily ration, feeding quantity will make the living body bait of water body reach 2-3/L；
During step 10, larval culture, water quality requirement：19-28 DEG C of water temperature, salinity 23-30 ‰, pH value 6.8-7.8；Nursery pond is inhaled
Bottom, which is removed contamination, to be changed to 1 time every other day, and it is identical with during hatching that other change water method, environmental requirement；By the cultivation of three weeks, cuttlefish seedling trunk
Body length is sold with regard to that can reach more than 2cm as commercial seedling.
2. the method for culturing seedlings of tiger spot cuttlefish as claimed in claim 1, it is characterised in that in the step 5, hatched using shallow water
Water method is changed in tidal bulge lakie in scheme, and natural imitation circle, and again and again continuous 6h is at the uniform velocity intake or at the uniform velocity draining.
3. the method for culturing seedlings of tiger spot cuttlefish according to claim 1, it is characterised in that thrown twice sooner or later in the step 9
The time for feeding bait is respectively 05：00、19：00.
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| CN106841519A (en) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | A kind of screening technique of tiger spot cuttlefish open-mouthed bait nutrition fortifier and its application |
| CN109874704A (en) * | 2019-03-29 | 2019-06-14 | 宁波大学 | A kind of method of tiger spot cuttlefish seed bait domestication |
| CN109864019B (en) * | 2019-04-19 | 2022-04-15 | 中国水产科学研究院黄海水产研究所 | Hatching system for fertilized eggs of sepia reiliana and using method |
| CN110402851B (en) * | 2019-08-28 | 2022-01-11 | 浙江海洋大学 | Method for breeding cuttlefish with tiger spot |
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| CN102919166A (en) * | 2011-08-12 | 2013-02-13 | 湛江师范学院 | Artificial egg taking method for sepia pharaonis |
| CN102428884A (en) * | 2011-09-19 | 2012-05-02 | 宁德市南海水产科技有限公司 | Large-scale total artificial breeding method of Sepiella maindroni |
| CN202697472U (en) * | 2011-12-14 | 2013-01-30 | 浙江海洋学院普陀科学技术学院 | Tidal incubation bucket device |
| CN102939924B (en) * | 2012-11-13 | 2014-07-23 | 宁波大学 | Method for artificially breeding sepia lycidas gray |
| CN103548721B (en) * | 2013-10-17 | 2015-09-02 | 宁波大学 | A kind of breeding method of tiger spot cuttlefish large size seedling seed |
| CN103548733B (en) * | 2013-10-30 | 2016-06-22 | 浙江海洋学院 | Tiger spot sepiellae seu sepiae method for culturing seedlings |
| CN104041449B (en) * | 2014-04-04 | 2016-01-13 | 复旦大学 | The Sungkiang weever of simulate natural ecological environment breeds cultivating workshop system |
| CN104273074B (en) * | 2014-10-31 | 2017-07-28 | 岭南师范学院 | A kind of method for cultivating cuttlefish seedling |
| CN104472406B (en) * | 2014-12-03 | 2017-07-18 | 宁波大学 | Cement pit cultural method in a kind of tiger spot cuttlefish room |
| CN204350866U (en) * | 2014-12-19 | 2015-05-27 | 山东省海洋生物研究院 | Tide simulation fluctuation rule enters the mud snail indoor culture device of draining automatically |
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