KR102010192B1 - A useful as live-food copepod, Apocyclops royi of aquaculture species and mass culturing method - Google Patents

Abstract

The present invention relates to a method for culturing Apocyclops royi, a novel food organism applicable to early stages of useful marine aquaculture varieties. Through the cultivation method according to the present invention, it is possible to solve instability of artemia supply in an aquaculture industry by replacing an existing food organism, artemia, which totally depends on imports, and to secure the quality of Apocyclops royi. Therefore, it is possible to greatly contribute to the enhancement of the growth and survival rate of early life cycles of aquaculture varieties.

Description

A useful as live-food copepod, Apocyclops royi of aquaculture species and mass culturing method}

The present invention relates to a mass culture method of Apocyclops royi useful as a food organism of aquaculture culture varieties.

The stable supply of food organisms in the production of artificial seedlings of aquaculture is an important determinant of their growth and survival in the early stages. In general, in the production of seedlings, the feed family of animal feed supplies rotifers in the early stages of larvae and crustacean larvae, and the larvae that hatch the cysts of Artemia in later stages. Rotifer ( Brachionus plicatilis , 130-340㎛ and B. rotundiformis , 100-210㎛) have developed high density mass culture technology, so there is no big problem in feeding during seedling production, and Artemia can be stored in cyst form for long-term storage. It is widely used all over the world because it can be easily hatched and supplied as food when needed.

However, artemia cysts are collected in the natural state and commercialized. If environmental problems occur, their production cannot be predicted, which can lead to phenomena such as supply and demand imbalances and price fluctuations. Artemia, which is used in more than 20,000 seedling production markets worldwide, consumes about 2,000 tons per year, most of which is known to be produced in the Great Salt Lake in the United States. Recently, however, supply and demand problems have arisen because the production of the Artemia habitat has not been predicted due to indiscriminate development and opposition from environmental groups, El Niño and abnormal weather. In fact, from the late 1990s to the early 2000s, the production of artemia cyst remained at the 10% level in the past, resulting in an increase in supply prices by an average of 5 to 6 times, putting pressure on the domestic aquaculture industry. In addition, the Artemia cyst recently produced in some Southeast Asia will gradually limit the export products, except for domestic seed production, the problem is very serious.

In this situation, 100% of the artemia is imported and used in Korea, so it is expected that there will be a lot of difficulties in supplying and receiving artemia due to the international environmental change. to be.

In order to solve this problem, researches on new food organisms to replace artemia have been actively conducted. Amphipods, cladocera and copepoda are mainly studied as candidate foods. But unipods have some promising species ( Gammarus pulex , Byblis japonicus and Eriopisa chilkensis ) was cultured on a laboratory scale, but the developmental stage to adulthood through metamorphosis is so long that mass cultivation is impossible, and it is not widely used because it is known to be very demanding in cultivation conditions such as mainly consuming organic substances in water. In addition, since crustaceans reproduce asexually compared to monopods and copepods, generational alternation is faster and growth rate is higher than that of other candidate species. It is not a suitable food organism because it dies due to reduced vitality.

On the other hand, copepods can be supplied according to aquaculture varieties due to their various life spans (larvae nauplius → middle stage copepodite → adult adult). Ingredients (proteins, calcium, pigments, vitamins, trace elements, fatty acids, etc.) are known to be superior to any food organism. In particular, n-3 HUFA (EPA, DHA, etc.), which is an essential fatty acid for marine aquaculture species, is a very important nutrient. Although it is supplied through nutritional enhancement, copepods have a higher content of essential fatty acids than nutrient-enhanced artemia even without nutritional enhancement. In fact, it is reported that there is an excellent effect on the growth and survival rate of various fish and crustaceans as well as the increase in metamorphosis and color pigmentation rate.

There are several reasons why copepods with such various advantages have not been widely used in marine farming in place of Artemia. Since the nature of the collection of copepods is mainly collected through the net in nature, the amount of collection is also very unstable due to the influence of seasons and weather, and there is a danger of harmful parasitic organisms (pirates) that are caught during the collection. The use of copepods in artificial seedling production sites was extremely limited.

If a single species of copepod is secured and the mass cultivation technology is established accordingly, copepods with various advantages can have a very positive effect on the production of artificial artificial seedlings.

From this point of view, the development of new feed organisms using single copepods is inevitably required as a way to replace Artemia, which is widely used as a prey.

In order to develop new animal feed organisms, it is necessary to identify various environmental conditions such as salinity, water temperature, etc., so that they can be used in aquaculture farms. In particular, the part of food is important, and it is necessary to provide an economical source of food as it is reported that the food supply costs account for about 87% of the total production cost in the artificial mass culture process. However, in the case of copepods, it is known that it is not cultivated by the commercially available concentrated chlorella , which is cheaply used for the culture of rotifer and artemia , but only by the same species as Tetraselmis sp. There is a need to provide an alternative source of economic and efficient food.

The present invention has been made to solve the above problems, and aims to develop a new food organism that can replace Artemia supplied in the early life history stages of the marine culture breed, and to use it industrially in the production of artificial seedlings. .

In addition, the present invention to reduce the production cost of the food source generated during mass production by newly developing a food source of Apocyclops royi , a radish copepod attracting attention as an alternative food organism of Artemia, It is an object of the present invention to provide a culture method that can see the effect even without performing lipid nutrition enhancement. In addition, the developed food source can offer the possibility of mass cultivation on a scale of 20 liters or more.

In the present invention includes the step of culturing Apocyclops royi ,

Tetraselmis as food suecica ) And Apocyclops royi using a mixture of photosynthetic bacteria Provide a culture method.

In addition, the present invention provides a culture method for supplying Apocyclops royi cultured by the above-mentioned culture method as a food for marine aquaculture varieties.

In addition, the present invention includes the step of culturing Apocyclops royi ,

Tetraselmis as food suecica ) And using a mixture of photosynthetic bacteria,

The culture is carried out by Apocyclops royi performed in a culture vessel of 10 liters or more. Provided is a mass culture method.

In the present invention, Apocyclops royi , a new food organism applicable to the early stages of aquaculture useful aquaculture varieties, was isolated and the optimal culture conditions were determined to suggest a mass culture technique.

Particularly, in the present invention, the economical food which can be grown in a large amount in a cultivation process has been sought, thereby reducing the production cost.

Apocyclops Roy according to the present invention royi ) and its mass cultivation techniques can replace the existing food organism, Artemia, which relies on imports, to solve the instability of the supply of althemia in the aquaculture industry, and to secure new foods with high quality. It can provide useful effects, such as a significant contribution to growth and survival rates in the early stages of life cycle of aquaculture.

1 shows copepods ( Apocyclops) royi ) is a micrograph showing the morphological characteristics.
2 shows copepods ( Apocyclops) royi ) is a graph of growth curve according to salinity conditions.
Figure 3 Apocyclops royi ) is a graph of growth curve according to water temperature conditions.
4 shows copepods ( Apocyclops) royi ) is a graph of growth curve according to food type.
Figure 5 Apocyclops royi ) (a) Population density change graph of copepod larvae during large-scale cultivation.
6 shows copepods ( Apocyclops) royi ) is a graph of the change in total population of copepods (b).

The present invention is a single species of copepods ( Apocyclops Roy) royi ) species, and a technique for mass cultivation in a conventional artificial seedling production plant as a prerequisite for replacing artemia , and the optimum conditions for mass cultivation were identified, and the optimum conditions for Verified by mass culture experiment.

EMBODIMENT OF THE INVENTION Hereinafter, the structure of this invention is demonstrated concretely.

The present invention is Apocyclops Roy royi , A. royi ) of the Apocyclops Roy ( A. royi ) comprising the step of culturing It relates to a culture method.

In the present invention, Apocyclops Roy ( A. royi ) is available as a food in the early stages of fish larvae, fry and crustacean larvae useful marine products.

A. royi belongs to Arthropoda, copepod or Copepoda, Cyclopoida and Apocyclops, and its size is at least 110 μm and at most 1130 μm. to be. The A. royi is very useful as a food in the marine aquaculture market because the content of DHA, etc. is very excellent compared to Artemia.

As the Apocyclops Roy ( A. royi ) is a species belonging to the subangular subclass, as shown in Figure 1, the entire body has a crust, and the growth from the larval stage to the adult through the peeling of the crust. The larvae (pictured on the left) had a relatively circular body shape, and the borders of the head and chest were unclear, but the adults (pictured on the right) had a segmented body, indicating that the body was divided by the boundary.

The adult of Figure 1 (pictured right) is a female with a lot of round eggs on both sides of the lower part of the body is usually attached as one egg called a sac (egg sac, egg sacs) without this object is usually a male The probability is high. In addition, it has one red eye (eye) in the upper middle part of the body and performs food gathering and swimming functions due to appendages that appear like branches throughout the body.

Looking at the morphological characteristics of the A. royi ( A. royi ), the habit of swimming in the water of the A. royi is freely bouncing like water flea without regular or circular motion. When light is given, the movement is slowed down by the stimulus, and it appears to be concentrated in the lower and middle layers rather than the surface layer of the culture medium. The copepod breeding method is sexual reproduction, and when males mate with females and fertilize in females, cyst sacs are attached to both sides of tails. A large number of eggs are attached to each other, and when the eggs are mature enough, the female hatches the eggs by dropping them. Even freshly hatched larvae do not sink to the bottom because of their poor swimming function, and usually grow through molting at each stage of development (larvae → intermediate → adult) every 1 to 2 weeks.

The present invention can provide a method for culturing such Apocyclops Roy ( A. royi ). The culture may be incubated in the incubator after inoculating A. royi in the culture. A. royi according to the present invention is suitable for mass culture, where the mass culture may mean a scale of 10 L or more, or 20 L or more.

In one embodiment, Tetraselmis suecica as food for the culture And mixtures of photosynthetic bacteria can be used. The photosynthetic bacteria are carbonate assimilated bacteria using light energy, such as higher plants, and can be easily obtained on the market, thereby securing economic feasibility and increasing the population of A. royi in culture. In particular, the mixture can be used as a food to further enhance the lipid nutrients (DHA, n-3 HUFA, etc.) in A. royi . In the case of Artemia (including rotier), which is an existing food organism, this process is omitted in the A. royi of the present invention, as well as the quantitative and qualitative excellence at the individual level. Can be met at the same time.

The photosynthetic bacteria may be at least one selected from the group consisting of the genus Schizochytrium sp. And the genus Crypthecodinium sp.

Also, Tetraselmis suecica ) And the mixing ratio of photosynthetic bacteria may be 1: 0.1 to 1: 2, 1: 0.5 to 1: 1.5 or 1: 1 to 1: 1.5 based on dry weight. In this range, it is possible to maximize the number of populations by cultivating efficiency, to produce a nutritious A. royi , and to improve the growth and survival rate of marine aquaculture species in the future.

In the culture of A. royi the feed, that is, the dosage of the mixture may be 1 to 5 mg / day, 1 to 3 mg / day or 2 mg / day by dry weight of the food per 1000 individual A. royi . Excellent culture efficiency in the above range can be maximized the population, it is possible to secure the economics.

In addition, in the present invention, in addition to the above-described mixture as a food, isochrysis galbana ( Isochrysis galbana ), Nannochloropsis Okulata ( Nannochloropsis oculata ), Chaetoceros calcitrans ) and Pavlova lutheri may further be used.

In one embodiment, the salinity of the culture in the culture may be 5 to 33 ‰, preferably 15 to 20 ‰. The culture efficiency is excellent in the conditions of the 15 to 20 ‰ can maximize the population, it is very advantageous for mass culture.

In one embodiment, the temperature in the culture may be 15 to 35 ℃, preferably 25 to 35 ℃, more preferably 25 to 30 ℃. The culture efficiency is excellent in the conditions of 25 to 35 ℃ can maximize the population, it is very advantageous for mass culture.

In addition, in one embodiment, the incubation period may be at least 15 days. If it is impossible to recover the culture solution in the incubator, it may be cultured for less than 20 days or less than 19 days, and if possible, the culture may be performed for 30 days or more.

It is possible to optimize the mass culture at the salinity and temperature conditions.

In one embodiment, the present invention comprises a step of inoculating Apocyclops royi in a culture solution having a salt of 15 to 20 ‰, followed by culturing at 25 to 30 ℃, the feed to tetracell Tetraselmis suecica ) And Apocyclops royi using a mixture of photosynthetic bacteria A culture method can be provided.

In addition, the present invention includes the step of culturing Apocyclops royi ,

Tetraselmis as food suecica ) And using a mixture of photosynthetic bacteria,

The culture is carried out in Apocyclops royi performed in a culture vessel of 10 liters (L) or more. Provided is a mass culture method.

In the mass culture method, the culture may be performed by the above-described culture method.

In addition, the present invention provides an aquaculture method for feeding Apocyclops royi cultured by the above-described culture method as a food for marine aquaculture varieties.

In the present invention, the marine aquaculture species may be fish larvae, larvae or invertebrate larvae.

The present invention can be applied to the marine culture of the early life history stage of marine aquaculture varieties, by using the Apocyclops royi as a food can contribute to the growth and survival rate of aquaculture breeds.

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, this is for the purpose of illustrating the invention and should not be used to limit the scope of the invention.

Example

Example  One. Apocyclops Roy ( Apocyclops royi )of  Gathering and Separation

Apocyclops royi (hereafter copepods) were collected and separated in the following manner.

Apocyclops royi ) was collected near and at a nursery test site in Haenam-gun, Jeollanam-do. Gathering was collected using 50 μm of mesh, 30 cm of netting, and 1 m of plankton net, and the collected samples were placed in a 500 ml water bottle and placed in an ice box. Samples transported to the laboratory were immediately identified by species under a dissecting microscope, extracted with a Kepler pipette, housed in a 500 ml glass beaker and incubated in a single culture. The initial culture saline was maintained at 25 ‰ as the collection site and placed in an incubator set at 24 ° C. The food was fed with a mixture of Tetraselmis suecica and Isochrysis galbana to 30,000 cells / ml, and then fed more than once a day. As the population increased in the beaker, it was housed in a new beaker to secure species and obtain samples for mass culture experiments.

Experimental Example  One. Apocyclops Roy ( Apocyclops royi )of  Morphological characteristics

(1) method

Apocyclops royi were extracted from the beaker in culture by using capillary pipette and placed on a slide glass, and morphological characteristics were observed under a dissecting microscope.

For the size measurement, 30 freshly hatched larvae (nauplius) and adults were isolated. Thereafter, a small amount of 5% neutral formalin was dropped on the slide glass, and the sample was fixed. Then, the average value was measured by a ruler mounted on an anatomical microscope eyepiece.

(2) results

Figure 1 is a copepod ( Apocyclops) in the present invention royi ) is a micrograph showing the morphological characteristics.

As shown in FIG. 1, copepods ( Apocyclops) royi ) has a small size of 110㎛ in the larval stage, the average adult size of 1130㎛ has a variety of advantages for each developmental stage.

Experimental Example  2. Apocyclops Roy ( Apocyclops royi )of  Optimization of culture conditions

(1) Saline condition optimization

① Method

In order to determine the optimal salinity, experiments were performed by selecting the salt conditions of 5, 10, 15, 20, 25, and 33 ‰ by mixing filtered natural seawater (33 ‰) and fresh water (0 ‰).

Specifically, each beaker was inoculated in a 250 ml beaker (150 ml of culture solution) so that the total density of copepods was 10 individuals, and the inoculated beaker was incubated for 16 days in an incubator maintained at 25 ° C. In the experiment, the plant feed organisms, Tetraselmis suecica and Isochrysis galbana , were mixed and fed once a day to have a concentration of 2 × 10 ⁵ cell / ml based on the culture medium. . Retrieval was not performed until the end of the experiment and the experiment was performed with three repetitions without aeration.

After the start of the experiment, the culture medium of each experimental section was extracted with a micropipette under a dissection microscope every day, and the number of individuals was counted based on 1 ml. The experimental results were finally derived by converting the total number of individuals.

② result

The experimental results are shown in FIG. 2.

2 shows copepods ( Apocyclops) royi ) is a graph of growth curve according to salinity conditions.

As shown in Figure 2, it can be seen that the population tends to increase than the initial inoculation density until the end of the experiment in all salinity test intervals. Through this, it can be confirmed that the species of the present invention is a salt salt resistance photo-inflammatory species. However, the highest density was found to be the highest at 120 ‰ in 15 ‰ and 20 ‰ at 10 to 11 days of culture. Therefore, the proper salinity condition for mass culture was determined to be 15-20 ‰.

In summary, the species of the present invention is characterized by easy artificial cultivation as photo-inflammatory (euryhaline, 廣 鹽 性) and salinity range from 5 to 33 ‰, but the optimum culture salinity is 15 to 20 ‰ In other salinity conditions, it is a salt range which can maintain and preserve a species rather than mass culture.

(2) Optimization of water temperature conditions

① Method

In the culture experiment according to the water temperature, 15 mL of salt was prepared in a 250 mL beaker (150 mL of culture solution) in a multichannel incubator adjusted to 15, 20, 25 and 30 ° C, and inoculated so that the copepod density totaled 10 individuals. . The food was fed to Tesselmismis suecica and isolysis mixed to 2 × 10 ⁵ cell concentration / ㎖ once a day, the return was not allowed until the end of the experiment. The experiment was repeated three times for 16 days, and was not aerated due to fear of damage to the appendages of copepods. As in the salinity experiment, the experimental results were counted daily under a dissecting microscope and converted into total populations.

② result

The experimental results are shown in FIG. 3.

Figure 3 Apocyclops royi ) is a graph of growth curve according to water temperature conditions.

As shown in FIG. 3, two experimental sections (25, 30 ° C.) and two experimental sections (15, 20) which did not show a big difference in all the experimental sections until the fifth day of culture but grew well from the eighth day of culture. ℃)) showed a distinct difference.

The highest density was highest at 125 ° C. at 30 ° C. on day 12, followed by 95 at 25 ° C .. On the other hand, 15 and 20 ℃ were 40, 60 individuals, respectively, showing low population density, showing a difference in growth according to water temperature.

Therefore, the water temperature conditions for mass culturing the species of the present invention is 25 to 30 ℃, it is judged that 30 ℃ is more preferable. However, as in the salinity experiment, it was found to be higher than the initial individual density at a relatively low temperature of 15 ℃ and a high temperature of 30 ℃, it was confirmed that it has the characteristics of photothermal (eurythermal, 廣 溫 性), which is easier than when artificial culture It means.

(3) optimization of feeding conditions

① Method

To examine the growth according to food type, Tetraselmis suecica (TET), Isochrysis galbana (ISO), and Nannochloropsis Oculata oculata , NAN), Chaetoceros calcitrans , CHA) and Pavlova lutheri (PAV) were prepared in advance and used for the experiment. In order to secure a more economical source of food, Schizochytrium , a plant food organism, is easily available on the market, Tetraselmis sueseica. sp.) mixed feed experiment (MIX) was used together in the experiment.

The experiment was prepared by inoculating a total of 600 individuals with a salt of 15 ‰ in a 250 ml beaker (150 ml of culture solution), and inoculated into 600 individuals, and the experiment was performed in an incubator at 30 ° C. The food supply amount was supplied once a day based on the dry weight of 2 mg of food per 1,000 copepods. The experiment lasted 25 days and all experiments were repeated three times. The population was counted in the same way as the water temperature and salinity experiments, and the result was calculated by converting the total population value.

Meanwhile, in order to analyze fatty acids (nutrition) of copepods according to food type, copepods in the beaker of each experimental section were filtered with 40 μm Mullergaz after sampling was completed, and stored at -80 ° C. Thereafter, the frozen samples were lyophilized to be powdered and quantified by electronic balance, and then lipid was extracted with an organic solvent. Next, the pretreatment was performed and finally the fatty acid was analyzed by gas chromatography apparatus.

The species of the present invention is a novel feed organism to replace the existing food organism, Artemia, so that a control diet of two kinds of altemia nutrient-enhanced for 3 hours and lipid-enriched Altemia as a lipid nutrient enhancer was put together. Analyzes were made with 6 jobs.

② result

First, the growth (object density change) experiment results of copepods according to food type are shown in FIG. 4.

4 shows copepods ( Apocyclops) royi ) is a graph of growth curve according to food type.

As shown in FIG. 4, the species of the present invention was shown to be greatly affected by the type of food, showing a large difference in individual density change in each experimental section from the beginning of the culture. The highest density was highest at 1245 and 1270 in TET (tetraselmis suesica monosphere) and MIX (tetraselmis suesica + photosynthetic bacterial mix) at 19 days of culture. Next, CHA showed a relatively high individual density until the 13th day, while the other experiments tended to have a lower density. However, it was judged that it could be used as a supplementary food because the density of individuals was not lost immediately after feeding, but maintained even though the density of individuals was low for more than three weeks.

In addition, the fatty acid analysis results of copepods according to food type are shown in Table 1 below.

¹ UEN, Artemia nauplius before nutrition;

² ENA, 3-hour enriched Altemia larvae;

³TET, tetraselmis suezica (Tetraselmis suecica) Supply port;

⁴ MIX, TET (50%) + photosynthetic bacteria ( Schizochytrium sp., 50%) feed port;

⁵ CHA, Chaetoceros calcitrans ) feed port ;

⁶ ISO, Isochrysis galbana ) feed port ;

⁷ NAN, Nannochloropsis oculata ) feed port ;

⁸ PAV, Pavlova lutheri ) supply port .

⁹ Saturated fatty acid; 6: 0, 8: 0, 10: 0, 11: 0, 12: 0, 13: 0, 14: 0, 15: 0, 16: 0, 17: 0, 18: 0, 20: 0, 21: 0.

¹⁰ Mono unsaturated fatty acid (sum of monounsaturated fatty acids); 15: 1, 16: 1, 17: 1, 18: 1 n9, 20: 1, 22: 1 n-9, 24: 1

¹¹ highly unsaturated fatty acid; 18: 2n-6, 18: 3n-3, 20: 2, 18: 3n-6, 20: 3n-6, 20: 4n-6, 20: 5n-3, 22: 2, 22: 6n-6

¹² Eicosapentaenoic acid (Eicosapentaenoic acid, IPA);

¹³ Docosahexaenoic acid.

As shown in Table 1, the DHA content absolutely required for marine fish larvae (larvae and larvae) and crustacean larvae was not detected before nutrition (UEN) in the existing food organism Artemia, and after nutrition fortification It was found to have a content of 15.5%. On the other hand, the experimental group fed the type of food to the copepods of the present invention MIX was found to show the highest content of 37.3% even though the nutrition was not strengthened. Due to this, the content of n-3 HUFA (the total sum of polyunsaturated fatty acids of omega 3) was also the highest at 50.0%, resulting in very high quality results. These results showed higher values than the experimental section of copepods fed other foods. It is believed that more DHA content was accumulated in the body due to the food when ingested MIX food.

On the other hand, in the experimental section of copepods fed other foods, the DHA content was higher than the ENA control, which was nutritionally fortified with the existing food organism, Artemia, even though it was not nutritionally fortified. It can be confirmed that it has a high content of DHA.

Therefore, photosynthetic bacteria ( schizochytrium) is relatively cheaper than cultivating TET, a plant food organism, and supplying to copepods of the present invention. sp.) can be economically cultivated in this species when mixed and fed. In addition, it is possible to satisfy the economics and efficiency at the same time as the qualitative quality of existing food organisms (Artemia) without the nutrition enhancement when feeding the mixed food, thereby improving the growth and survival rate in the early stages of marine aquaculture species. Produce results that are worthwhile.

Experimental Example  3. Apocyclops Of Apocyclops royi  Bulk culture (20ℓ scale)

(1) method

The cultivation conditions identified in Experimental Example 2 described above, that is, the bulk culture was carried out at 20 L scale, not the beaker level, by applying the salt 15 ‰ and water temperature 30 ℃ conditions. At this time, photosynthetic bacteria were mixed with tetraselmis suecica and fed as a food source.

The whole culture solution was mixed well every day, and the culture solution was uniformly extracted with a micropipette, and the total population including nauplius and larvae was counted under a dissecting microscope. The resultant was then divided into larvae and total populations.

(2) results

The results are shown in FIGS. 5 and 6.

Figure 5 Apocyclops royi ) (a) population density change of copepod larvae during mass cultivation, Figure 6 is apocyclops royi ) is a graph of the change in total population of copepods (b).

As shown in FIG. 5 and FIG. 6, the larvae of copepods showed a tendency to increase steadily every day from the beginning of the experiment to the end of the experiment, thereby increasing the total population. In particular, from the middle of the experiment, the larval water was steadily high by 10 or more per ml, and the mass culture proceeded smoothly. At the beginning of the experiment, the total population was 100,000, whereas at the end of the experiment, the total population increased to 473,330, increasing 4.7 times in three weeks.

These results confirm that the copepod of the present invention is capable of mass cultivation at a scale larger than the beaker level.

Claims (10)

Culturing Apocyclops royi ,
Tetraselmis suecica as food And using a mixture of photosynthetic bacteria,
Tetraselmis suecica And the mixing ratio of photosynthetic bacteria is 1: 0.1 to 1: 2 based on dry weight of Apocyclops royi . Cultivation method.
The method of claim 1,
Photosynthetic bacteria of the Apocyclops royi comprising at least one selected from the group consisting of Schizochytrium sp. And Crypthecodinium sp. Cultivation method.
The method of claim 1,
A method for culturing Apocyclops royi , which feeds 1.0 to 5.0 mg / day per 1000 individuals.
The method of claim 1,
Feeding isocrysis galvana galbana ), Nannochloropsis oculata , and Chaetoceros calcitrans ) and Pavlova lutheri . The method of culturing Apocyclops royi further comprising at least one selected from the group consisting of:
The method of claim 1,
The method of culturing Apocyclops royi salinity of the culture solution in culture is 15 to 20 ‰.
The method of claim 1,
Method of culturing Apocyclops royi is a temperature of 25 to 35 ℃.
The method of claim 1,
Cultivation period of 15 days or more Apocyclops royi ( Apocyclops royi ) culturing method.
An aquaculture method for feeding Apocyclops royi cultured by the culture method according to claim 1 as a food for marine aquaculture varieties.
The method of claim 8,
Marine aquaculture is a method of farming in which larvae, fry or invertebrates of fish are larvae.
Culturing Apocyclops royi ,
Tetraselmis suecica as food And using a mixture of photosynthetic bacteria,
Tetraselmis suecica And the mixing ratio of photosynthetic bacteria is 1: 0.1 to 1: 2 based on dry weight,
The culture is carried out in Apocyclops royi performed in a culture vessel of 10 liters (L) or more. Mass culture method.

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