CN107950441A - A kind of Tilapia mossambica salt water cultural method - Google Patents

A kind of Tilapia mossambica salt water cultural method Download PDF

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Publication number
CN107950441A
CN107950441A CN201711452998.9A CN201711452998A CN107950441A CN 107950441 A CN107950441 A CN 107950441A CN 201711452998 A CN201711452998 A CN 201711452998A CN 107950441 A CN107950441 A CN 107950441A
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China
Prior art keywords
tilapia mossambica
salt water
salt
train
cultural method
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CN201711452998.9A
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Inventor
李芳远
佟延南
李高俊
赵光军
朱海
王德强
申志新
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Hainan Academy Of Ocean And Fisheries Sciences (hainan Ocean Development Planning And Design Research Institute)
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Hainan Academy Of Ocean And Fisheries Sciences (hainan Ocean Development Planning And Design Research Institute)
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Priority to CN201711452998.9A priority Critical patent/CN107950441A/en
Publication of CN107950441A publication Critical patent/CN107950441A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of Tilapia mossambica salt water cultural method, belong to technical field of aquaculture.Tilapia mossambica salt water cultural method disclosed by the invention includes:1) Tilapia mossambica salt tolerant is raised and train;2) salt water cultivates pool cultivated two steps, and specifically, Rofe fry salt tolerant reduces or stop feeding during raising and train.Tilapia mossambica salt water cultural method survival rate of fish fry disclosed by the invention is up to more than 90%, the instantaneous rate of body weight gain of Tilapia mossambica (IGR) and absolute gain rate (AGR) that salt water harvests after cultivating 120 days be not notable with freshwater aquiculture difference, and Tilapia mossambica bilgy odour is effectively reduced.

Description

A kind of Tilapia mossambica salt water cultural method
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of Tilapia mossambica salt water cultural method.
Background technology
Tilapia mossambica (Oreochromis mossambila), is commonly called as African crucian, non-crucian carp, Vietnam fish, Nan Yang crucian carp etc., is subordinate to In Perciformes, Percoidei, Callichthyidae, Tilapia mossambica category, the mouth that original refers to using Mozambique as type locality incubates non-crucian carp category fish thing Kind:Non- crucian carp (scientific name is incubated in Mozambique mouthful:Oreochromis mossambicus), now non-crucian carp is incubated for the non-crucian carp category of Family Cichlidae and mouth Category etc. belongs to being commonly called as jointly for several fish;Tilapia is referred to as in English.Tilapia mossambica feeding habits are extensive, are mostly based on vegetalitas Omnivorousness, very voracious, food ration is big;Growth is rapid, especially with young stage growth faster.Growth Op Tilapia has close pass with temperature System, 16~38 DEG C of growth temperature, 22~35 DEG C of thermophilic.It is a kind of eurysaline fishes, can all survives in sea water and fresh water;Lower oxygen concentration resistance, one As inhabit the lower floor of water, but change with water temperature change or fish body size and inhabit water layer.
Tilapia mossambica protein content is high and be easily digested, nutritive value it is very high, enjoy " plain boiled pork salmon " Good reputation.The whole world has a countries and regions more than 100 to carry out Rofe fish culture at present, and wherein China is Rofe fish culture big country, The ground such as Guangdong, Guangxi, Fujian and Hainan cultivating large area.Tilapia Fillet has been the brand production in Chinese exports agricultural product Product.
Wherein, bolti (Oreochromis niloticus) accounts for more than the 90% of Tilapia mossambica total output, from 1978 China has repeatedly been introduced since year, becomes one of Chinese main cultured fishes, its growth is fast but its salt tolerance is not strong, it is impossible in seawater Middle cultivation, is essentially limited to the breeding production in poisons in freshwater.There are water surface area and water resource be limited, water quality for freshwater aquiculture The problems such as being susceptible to environmental pollution, the fish crop obtained every year are difficult to meet the needs of market.Also, the Rofe of freshwater aquiculture Fish often has bilgy odour, and flavor is not good enough.As China faces fresh water to the pay attention to day by day of freshwater resources and environmental protection, aquaculture The situation of shortage of resources is also increasingly prominent.At the same time, China still has the low-lying Salt lake facies in a large amount of inlands.
Therefore, the method for developing the cultivation of Tilapia mossambica salt water is very important.
The content of the invention
In order to solve the above technical problems, the present invention provides a kind of Tilapia mossambica salt water cultural method, Rofe of the present invention Fish salt water cultural method survival rate of fish fry is high, the speed of growth and freshwater aquiculture difference are little, and bilgy odour is effectively reduced.
The present invention solves technical solution used by above-mentioned technical problem:
The present invention provides a kind of Tilapia mossambica salt water cultural method, comprises the following steps:
1) Rofe fry salt tolerant is raised and train 8-10 days;
2) the Rofe fry raised and train by step 1) launches salinity control and is cultivated in 8 ‰ -12 ‰ salt water aquaculture pond;
Wherein, reduced during the Rofe fry salt tolerant described in step 1) is raised and train or stop feeding.
Preferably, step 1) improves the progress salt tolerant of salinity 0.5 ‰ -2 ‰ and raises and train daily.
Preferably, the Rofe fry initial weight described in step 1) is 5-10 grams/fish.
It is highly preferred that the Rofe fry scale, fin ray are complete, no wound is energetic.
In a kind of embodiment of the present invention, it is that cement pit is raised and train that the step 1) salt tolerant, which is raised and train, works as cement pit Specification is 6.0m × 5.0m × 1.6m.
Specifically, step 1) is described raises and train period, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.
Preferably, it is rapid 1) described in Rofe fry salt tolerant raise and train during before three/a period of time stop feed, centre three / start to feed for a period of time, rear 1/3rd period returns to normal feeding volume.
Preferably, during the Rofe fry salt tolerant described in step 1) is raised and train, after salinity is raised and train more than 6 ‰, slow down tame and docile Speed is supported, the progress salt tolerant of salinity 0.5 ‰ -0.8 ‰ is improved within every 1 day and raises and train.
In a kind of embodiment of the present invention, the salt water is sea ice water and/or bittern water and/or sea salt and ground The salt water that lower water configuration forms.
Preferably, the salt water salinity is 8 ‰ -12 ‰.
Salt water of the present invention can be dissolved in underground water by sea salt to obtain the salt water or seawater and ground Lower water configuration forms.
Salt water salinity of the present invention is measured by WYY-1 type light refractive types salinometer.
The present invention has the following advantages compared with prior art:
1st, Rofe fry salt tolerant is raised and train and is organically combined with salt water cultivation, survival rate of fish fry is high;Avoid Tilapia mossambica The survival rate of fish fry caused by the difference of time, space, weather etc. is low between the domestication of seedling salt tolerant and large-scale cultivation asks Topic.
2nd, the Rofe fry salt water cultivation survival rate raised and train through salt tolerant is up to more than 90%.
3rd, Tilapia mossambica salt water cultural method fish crop disclosed by the invention is suitable with freshwater aquaculture method yield, has reality Economic implications.After raising in 120 days, Buddhist nun sieve's Rofe of Tilapia mossambica salt water cultural method cultivation harvest disclosed by the invention Instantaneous rate of body weight gain (IGR) and absolute gain rate (AGR) of the fish in salt water are respectively 2.1 (%/d) and 465.34 (mg/d), right According to group, instantaneous rate of body weight gain (IGR) and absolute gain rate (AGR) in fresh water are respectively 2.12 (%/d) and 470.23 (mg/ D), the two growth differences is not notable (p ﹥ 0.05).
4th, Tilapia mossambica salt water cultural method disclosed by the invention may not only be applied to coastal area, low-lying available for China inland Salt lake facies, especially NORTHWEST CHINA Salt lake facies Rofe fish culture;Tilapia mossambica cultured area can further be expanded, improve fish crop.
5th, compared with the Tilapia mossambica of poisons in freshwater cultivation, Tilapia mossambica salt water cultural method harvest disclosed by the invention Tilapia mossambica bilgy odour is effectively reduced, and Tilapia flavor is good, and flesh quality gets a promotion.
Embodiment
With reference to specific embodiment, the present invention will be further described.It is to be understood that within the scope of the present invention, the present invention Above-mentioned each technical characteristic and each technical characteristic for specifically describing in below (eg embodiment) between can be mutually combined, from And form new or preferable technical solution.As space is limited, not repeated them here.
Survival rate, instantaneous rate of body weight gain, the calculation bibliography of absolute gain rate:Li Sifa etc. is the Changjiang river, the Zhujiang River, black Longjiang silver carp flathead, grass carp research of fruit germplasm resource [M] Shanghai:Shanghai science tech publishing house, 1990.84-153.It is specific as follows:
Survival rate (%)=harvest number/puts number × 100% in a suitable place to breed
Instantaneous rate of body weight gain IGR (%/d)=[(lnW2-lnW1)/(t2-t1)] × 100%
Absolute gain rate AGR (mg/d)=(W2-W1)/(t2-t1)
W1, W2 are respectively the weight of t1 days and t2 days in formula.
In a specific embodiment of the present invention, water salinity is measured by WYY-1 type light refractive types salinometer.
As it is known by the man skilled in the art, monitoring water quality and aquaculture management should be paid attention in Tilapia mossambica breeding process.Specifically Cultivation skill and points for attention with reference to standard in the industry《Rofe fish culture quality safety management technical specification》、《Buddhist nun sieve's Rofe Fish culture technical specification fries, fingerling》.Detail no longer repeats one by one.
Embodiment 1
Fish is used in experiment:Bolti, treats that salt tolerant raises and train 5-7 grams of Rofe fry weight, scale, fin ray are complete, no wound, living Power is strong.
Experiment salt water:Sea crystal and underground water (salinity 0) configuration form, and water body salinity is 11 ‰.
Experiment tilapia feed is commercially available Tilapia mossambica special compound feed, the same standard of feed formula《Tilapia mossambica, which coordinates, to be raised Material》.
Tilapia mossambica salt water cultural method includes the following steps:
1) cement pit (6.0m × 5.0m × 1.6m specifications) seedling salt tolerant is raised and train 3 days, is put 200 tails in a suitable place to breed per pond, is improved salt daily 2 ‰ are spent, is not fed.
2) continue salt tolerant to raise and train 3 days, improve salinity 0.8 ‰ daily, gradually step up feeding volume, fed to normal within the 3rd day Amount, every morning change water 1 time, and quantity of exchanged water is the 50% of cumulative volume.
3) continue salt tolerant to raise and train 3 days, improve salinity 0.5g/L daily, periodically feed Tilapia mossambica special compound feed daily, Every morning changes water 1 time, and quantity of exchanged water is the 50% of cumulative volume.
4) the Rofe fry that step 3) is raised and train is launched salinity control to cultivate in the salt water aquaculture pond of 11g/L.Cultivation 120 days, detection survival rate, instantaneous rate of body weight gain, absolute gain rate.
Monitoring water quality and aquaculture management are paid attention in above-mentioned breeding process, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.
Embodiment 2
Fish is used in experiment:Bolti, treats that salt tolerant raises and train 8-10 grams of Rofe fry weight, and scale, fin ray are complete, no wound, It is energetic.
Experiment salt water:Sea salt and underground water (salinity 0) configuration form, and water body salinity is 11g/L.
Experiment tilapia feed is commercially available Tilapia mossambica special compound feed, the same standard of feed formula《Tilapia mossambica, which coordinates, to be raised Material》.
Tilapia mossambica salt water cultural method includes the following steps:
1) cement pit (6.0m × 5.0m × 1.6m specifications) seedling salt tolerant is raised and train 3 days, is put 100 tails in a suitable place to breed per pond, is improved salt daily 2g/L is spent, is not fed.
2) continue salt tolerant to raise and train 3 days, improve salinity 0.8g/L daily, gradually step up feeding volume, fed to normal within the 3rd day Amount, every morning change water 1 time, and quantity of exchanged water is the 50% of cumulative volume.
3) continue salt tolerant to raise and train 4 days, improve salinity 0.5g/L daily, periodically feed Tilapia mossambica special compound feed daily, Every morning, lower nothing respectively change water 1 time, and quantity of exchanged water is the 40% of cumulative volume.
4) the Rofe fry that step 3) is raised and train is launched salinity control to cultivate in the salt water aquaculture pond of 11g/L.Cultivation 120 days, detection survival rate, instantaneous rate of body weight gain, absolute gain rate.
Monitoring water quality and aquaculture management are paid attention in above-mentioned breeding process, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.Support Grow 120 days, detection survival rate, instantaneous rate of body weight gain, absolute gain rate.
Embodiment 3
Fish is used in experiment:Bolti, treats that salt tolerant raises and train 7-9 grams of Rofe fry weight, scale, fin ray are complete, no wound, living Power is strong.
Experiment salt water:Sea salt and underground water (salinity 0) configuration form, and water body salinity is 10g/L.
Experiment tilapia feed is commercially available Tilapia mossambica special compound feed, the same standard of feed formula《Tilapia mossambica, which coordinates, to be raised Material》.
Tilapia mossambica salt water cultural method includes the following steps:
1) cement pit (6.0m × 5.0m × 1.6m specifications) seedling salt tolerant is raised and train 3 days, is put 150 tails in a suitable place to breed per pond, is improved salt daily 1.5g/L is spent, is not fed.
2) continue salt tolerant to raise and train 3 days, improve salinity 0.8g/L daily, gradually step up feeding volume, fed to normal within second day Amount, every morning change water 1 time, and quantity of exchanged water is the 50% of cumulative volume.
3) continue salt tolerant to raise and train 3 days, improve salinity 0.5g/L daily, periodically feed Tilapia mossambica special compound feed daily, Every morning changes water 1 time, and quantity of exchanged water is the 50% of cumulative volume.
4) the Rofe fry that step 3) is raised and train is launched salinity control to cultivate in the salt water aquaculture pond of 10g/L.Cultivation 120 days, detection survival rate, instantaneous rate of body weight gain, absolute gain rate.
Monitoring water quality and aquaculture management are paid attention in above-mentioned breeding process, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.
Control 1
Fish is used in experiment:Bolti, 5-7 grams of fry weight, scale, fin ray are complete, no wound, energetic.
Test water:Underground water (salinity 0) is aerated 2 days.
Experiment tilapia feed is commercially available Tilapia mossambica special compound feed, the same standard of feed formula《Tilapia mossambica, which coordinates, to be raised Material》.
Tilapia mossambica fresh water control cultural method includes the following steps:
1) cement pit (6.0m × 5.0m × 1.6m specifications) is temporarily supported 3 days, is put 200 tails in a suitable place to breed per pond, is not fed.
2) continue temporarily to support 3 days, gradually step up feeding volume, every morning changes water 1 time, changes water to normal feeding volume within the 3rd day Measure as the 50% of cumulative volume.
3) continue temporarily to support 3 days, periodically feed Tilapia mossambica special compound feed daily, every morning changes water 1 time, and quantity of exchanged water is The 50% of cumulative volume.
4) the Rofe fry that step 3) is raised and train is launched in fresh water water aquaculture pond and cultivates.Cultivation 120 days, detection survives Rate, instantaneous rate of body weight gain, absolute gain rate.
Monitoring water quality and aquaculture management are paid attention in above-mentioned breeding process, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.
Control 2
Fish is used in experiment:Bolti, 8-10 grams of fry weight, scale, fin ray are complete, no wound, energetic.
Test water:Underground water (salinity 0) is aerated 2 days.
Experiment tilapia feed is commercially available Tilapia mossambica special compound feed, the same standard of feed formula《Tilapia mossambica, which coordinates, to be raised Material》.
Tilapia mossambica fresh water control cultural method includes the following steps:
1) cement pit (6.0m × 5.0m × 1.6m specifications) is temporarily supported 3 days, is put 100 tails in a suitable place to breed per pond, is not fed.
2) continue temporarily to support 3 days, gradually step up feeding volume, every morning changes water 1 time, changes water to normal feeding volume within the 3rd day Measure as the 40% of cumulative volume.
3) to continue temporarily to support 4 days, periodically feed Tilapia mossambica special compound feed daily, every morning, lower nothing respectively change water 1 time, Quantity of exchanged water is the 40% of cumulative volume.
4) the Rofe fry that step 3) is raised and train is launched in fresh water water aquaculture pond and cultivates.Cultivation 120 days, detection survives Rate, instantaneous rate of body weight gain, absolute gain rate.
Monitoring water quality and aquaculture management are paid attention in above-mentioned breeding process, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.
Control 3
Fish is used in experiment:Bolti, 7-9 grams of fry weight, scale, fin ray are complete, no wound, energetic.
Test water:Underground water (salinity 0) is aerated 2 days.
Experiment tilapia feed is commercially available Tilapia mossambica special compound feed, the same standard of feed formula《Tilapia mossambica, which coordinates, to be raised Material》.
Tilapia mossambica fresh water control cultural method includes the following steps:
1) cement pit (6.0m × 5.0m × 1.6m specifications) is temporarily supported 3 days, is put 150 tails in a suitable place to breed per pond, is not fed.
2) continue temporarily to support 3 days, gradually step up feeding volume, every morning changes water 1 time, changes water to normal feeding volume within second day Measure as the 50% of cumulative volume.
3) continue temporarily to support 3 days, periodically feed Tilapia mossambica special compound feed daily, every morning changes water 1 time, and quantity of exchanged water is The 50% of cumulative volume.
4) the Rofe fry that step 3) is raised and train is launched in fresh water water aquaculture pond and cultivates.Cultivation 120 days, detection survives Rate, instantaneous rate of body weight gain, absolute gain rate.
Monitoring water quality and aquaculture management are paid attention in above-mentioned breeding process, keeps 26-30 DEG C of water temperature, dissolved oxygen amount 6-8mg/L.
Result of the test
1 Tilapia mossambica salt water cultural method survival rate of table, instantaneous rate of increase, absolute growth rate (embodiment 1-3)
2 Tilapia mossambica salt water cultural method survival rate of table, instantaneous rate of increase, absolute growth rate (embodiment 1-3 average values)
Above result of the test shows:The bolti seedling salt water cultivation survival rate raised and train through salt tolerant is up to more than 90%; After raising in 120 days, the bolti of Tilapia mossambica salt water cultural method cultivation harvest disclosed by the invention is in salt water Instantaneous rate of body weight gain (IGR) and absolute gain rate (AGR) are respectively 2.1 (%/d) and 465.34 (mg/d), control group, in fresh water Instantaneous rate of body weight gain (IGR) and absolute gain rate (AGR) be respectively 2.12 (%/d) and 470.23 (mg/d), the two growth differences Not significantly (p ﹥ 0.05).
Tilapia mossambica salt water cultural method fish crop disclosed by the invention is suitable with freshwater aquaculture method yield, has reality Economic implications.
Reference literature (Hao Shuxian etc., the analysis of volatile components [J] of different breeding pattern Tilapia mossambica, food and fermentation work Industry, the 6th phase of volume 42 in 2016,147-152) detection test group and the control group Tilapia mossambica flesh of fish in enanthaldehyde, 2.4- heptadienals, Aldehyde C-9,2- decenals, 2,4- decadienals etc. cause the smell contribution margin of the volatile ingredient of fishlike smell and opposite smell activity Value.
Testing result shows that the smell contribution margin of the above-mentioned volatile ingredient of test group Tilapia mossambica flesh of fish fish and opposite smell are lived Angle value is significantly lower than control group.
Compared with the Tilapia mossambica of poisons in freshwater cultivation, sieve of Tilapia mossambica salt water cultural method harvest disclosed by the invention Non- fish bilgy odour is effectively reduced.
Above content is only presently preferred embodiments of the present invention, for those of ordinary skill in the art, according to the present invention Thinking, part can be changed in specific embodiment mode and its application range, this specification content should not be construed as Limitation of the present invention.

Claims (10)

1. a kind of Tilapia mossambica salt water cultural method, it is characterised in that comprise the following steps:
1) Rofe fry salt tolerant is raised and train 8-10 days;
2) the Rofe fry raised and train by step 1) launches salinity control and is cultivated in 8 ‰ -12 ‰ salt water aquaculture pond;Its In, reduced during the Rofe fry salt tolerant described in step 1) is raised and train or stop feeding.
2. the Tilapia mossambica salt water cultural method described in claim 1, it is characterised in that the daily raising salinity 0.5 ‰ of step 1)- 2 ‰ progress salt tolerants are raised and train, and process is raised and train in completion in 8-10 days.
3. the Tilapia mossambica salt water cultural method described in claim 1, it is characterised in that the Rofe fry described in step 1) initially weighs Measure as 5-10 grams/fish.
4. the Tilapia mossambica salt water cultural method described in claim 3, it is characterised in that the Rofe fry scale, fin ray are complete, It is energetic without wound.
5. the Tilapia mossambica salt water cultural method described in claim 1, it is characterised in that it is cement pit that the step 1) salt tolerant, which is raised and train, Raise and train.
6. the Tilapia mossambica salt water cultural method described in claim 1, it is characterised in that step 1) is described to raise and train period, keeps water Warm 26-30 DEG C, dissolved oxygen amount 6-8mg/L.
7. the Tilapia mossambica salt water cultural method described in claim 1, it is characterised in that the Rofe fry salt tolerant described in step 1) is tamed and dociled Three before during supporting/a period of time stopping nursing, centre three/start to feed for a period of time, rear 1/3rd period is extensive Normal feeding volume is arrived again.
8. the Tilapia mossambica salt water cultural method described in claim 1, it is characterised in that the Rofe fry salt tolerant described in step 1) is tamed and dociled During supporting, after salinity is raised and train more than 6 ‰, slow down and raise and train speed, improve within every 1 day the progress salt tolerant of salinity 0.5 ‰ -0.8 ‰ and tame and docile Support.
9. the Tilapia mossambica salt water cultural method described in claim 1-8 any one, it is characterised in that the salt water be seawater or The salt water that sea crystal is formed with underground water configuration.
10. the Tilapia mossambica salt water cultural method described in claim 9, it is characterised in that the salt water salinity is 8 ‰ -12 ‰.
CN201711452998.9A 2017-12-28 2017-12-28 A kind of Tilapia mossambica salt water cultural method Pending CN107950441A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113016670A (en) * 2021-03-30 2021-06-25 海南省海洋与渔业科学院 Tilapia mossambica saline water culture method

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CN103843701A (en) * 2013-12-23 2014-06-11 浙江省海洋开发研究院 Chinese black sleeper culture method

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JPH04325039A (en) * 1991-04-25 1992-11-13 Fujita Corp Culture of tilapia
CN101766140A (en) * 2010-01-27 2010-07-07 河北中捷罗非鱼养殖有限公司 Tilapia seawater acclimation method
CN103843701A (en) * 2013-12-23 2014-06-11 浙江省海洋开发研究院 Chinese black sleeper culture method
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113016670A (en) * 2021-03-30 2021-06-25 海南省海洋与渔业科学院 Tilapia mossambica saline water culture method

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