CN107455615A - A kind of artemia fortification method suitable for true octopus floating larva culture - Google Patents
A kind of artemia fortification method suitable for true octopus floating larva culture Download PDFInfo
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- CN107455615A CN107455615A CN201710597565.6A CN201710597565A CN107455615A CN 107455615 A CN107455615 A CN 107455615A CN 201710597565 A CN201710597565 A CN 201710597565A CN 107455615 A CN107455615 A CN 107455615A
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- artemia
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- floating larva
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Abstract
The invention discloses a kind of artemia fortification method suitable for true octopus floating larva culture, comprise the following steps：Preparation, extraction fish guts oil, fish oil modification, the fortification of tea polyphenol liposomes.Have the beneficial effect that：By extracting fish guts oil and carrying out enzymatic modification, saturated fatty acid in fish oil can be converted into unrighted acid, the content of the unrighted acid such as DHA, EPA in artemia body can be greatly improved, and make the artemia nutrition after reinforcing more comprehensively more balanced, the nutritional need of true octopus floating larva culture can be met, young lipid and aliphatic acid composition can be changed, improve the speed of growth of the young, survival rate, immunity with stress resistivity, therefore be it is a kind of efficiently, the artemia fortification method suitable for true octopus floating larva culture of safety.
Description
Technical field
The present invention relates to the aquatic products young to cultivate field, is sought more particularly, to a kind of artemia suitable for true octopus floating larva culture
Support intensifying method.
Technical background
Very high and steep is economic value is higher in high and steep class animal one kind, and most study, popularity highest head in the world
One of sufficient class, there is wide market in Mediterranean, Latin America and Asia.It is very high and steep that there is the characteristics of being suitable to cultivation below：1）
Efficiency of food conversion is high, and it can convert the 40-60% of digestion food；2）Growth rate is high, and its growth rate is about 3% daily；3）
Protein content is high, accounts for the 70-90% of dry weight；4）Fish brood amount is high, and each ripe individual fish brood amount is at 100,000-50 ten thousand.Because its is wide
The wealthy market space and excellent biological character, for many years numerous scholars to its ecology, physiology, behavior, breeding, growth and support
Grow and conduct extensive research, excellent basis is provided to carry out very high and steep cultivation.Trunk long 1- when the very high and steep young just hatches
1.5mm, they are travelling active, and metabolic rate is high, it is necessary to a large amount of nutritious living body baits.In the stage of swimming, their shapes
State change is obvious, and tentacle grows comparatively fast relative to rouge body portion, can gradually become the shape of adult from cuttlefish shape during hatching quickly
Shape.Bait is a vital factor in larval culture.Researcher is made up of the fat of the young and their foods
Foundation, the very high and steep food for needing to be rich in polyunsaturated fatty acid is proposed, especially to contain this 2 kinds of DHA, EPA, this is to weigh bait
Expect a key factor of quality height.
Artemia is constantly subjected to the extensive weight of people as a kind of important food organisms and good experimental animal material
The optimal selection eaten depending on, its naupiar larva for many tropical aquatiles first, in cultivation is put into practice, artemia be it is a kind of very
Preferable true octopus floating larva bait, although studies have found that fairy shrimp palatability is good, feed positive effect, the biochemistry of artemia
Composition lacks PUFA, the nutritional need of the very high and steep young can not be fully met, so as to cause the higher death of true octopus floating larva
Rate and the slower speed of growth, therefore true octopus floating larva bait is strengthened just become particularly important.
At present, the technical method on true octopus floating larva culture fortification is few, and prior art such as authorizes public number
For the B of CN 103141439 Chinese invention patent, the side of DHA, EPA and ARA in a kind of fortification artemia body are disclosed
Method, this method carrying out fortification by will be fed to artemia after euphausia supetba dana meal ultramicro grinding, having simple to operate, cost
The advantages of cheap, but its raw materials Antarctic krill has the characteristics of high protein, low fat, high mineral, it can
Supplemented the nutrients well for the growth of artemia, promote increasing for its vivo acid content, and can not improved in artemia body
The content of polyunsaturated fatty acid, and the saturated fatty acid in krill body can threaten to the health of artemia, therefore promote
Value is little.
The content of the invention
It is an object of the invention to provide a kind of efficient, safety artemia nutrition suitable for true octopus floating larva culture is strong
Change method.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：One kind is suitable to true octopus floating larva
The artemia fortification method of culture, comprises the following steps：
The preparation of tea polyphenol liposomes：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate that 0.04-0.05mol/L, pH are 7.5-7.8
Mixed solution 800-850 parts, Tea Polyphenols 8.0-8.1 parts are slowly added to, 58-60 DEG C is heated under strong stirring and keeps constant temperature；
Egg yolk lecithin 300-320 parts, cholesterol 58-60 parts, VE16-17 parts are weighed, is added in 500-600 part absolute ethyl alcohols, adds
Heat adds mixed solution in Tea Polyphenols solution after dissolving completely, keeping temperature strong stirring 30- to 58-60 DEG C of stirring and dissolving
After 45 minutes, under 55-60 DEG C of temperature, 0.1-0.15MPa vacuum rotary evaporation 20-25 minutes remove residual ethanol, Ran Houchao
Sonication, ultrasonic power 200-230W, frequency are 23-25 KHz, with ultrasonic 4 seconds intervals processing of the frequency ultrasound of 4 seconds 120-
180 seconds, tea polyphenol liposomes were placed in 3-4 DEG C of preservation by ultrasound after terminating；Tea polyphenol liposomes envelop rate prepared by the method is high,
It is easy to the absorption of artemia, and preparation method is simple, and the physics and chemistry containing potentially harmful substances such as ether is avoided in preparation process
Processing, it is especially suitable for industrialization large-scale production；
Extract fish guts oil：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner
Slurry, the homogenate of 200-210 parts fish guts is weighed into beaker, adds 60-65 part distilled water, stir lower warming-in-water to 30-34 DEG C,
PH to 8-9 is adjusted, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, adds fish guts weight 5-7% potassium chloride stirring
Saltout after dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, to enter one
Step modification is prepared；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into green high-efficient fertilizer total integration profit
With, be also used as extract nucleic acid raw material it is further high-valued, reached the purpose of waste's reclamation；
Fish oil is modified：Weigh crude fish oil 100-110 parts, vitamin C 0.07-0.08 parts are put in closed container and are well mixed, general
Reaction vessel is placed in Ultrasound Instrument, is heated to 70-73 DEG C and is kept, and is continually fed into oxygen 5-8 minutes, is then added desaturase
0.3-0.4 parts, reduced Coenzyme II 0.05-0.06 parts, make in the praetersonic that power is 400-500W, frequency is 80-90KHz
With stand-by after the lower reaction 110-140 seconds；Siphon enzymatic fish oil and with hot water wash 5-3 times, centrifuges, takes supernatant liquid while hot, with true
Empty drying box is drying to obtain fish guts oil；Enzymatic reaction can be seized the hydrogen of the centre position carbon-carbon double bond of saturated fatty acid by oxygen
Water is generated, so that chain saturated fatty acids lose hydrogen and are converted into corresponding unrighted acid, so that rich in fish guts oil
Containing polyunsaturated fatty acids such as DHA, EPA, the quality that artemia strengthens composition, desaturase and reduced coenzyme is highly suitable as
Than for 6-7:When 1, reactivity highest；
Fortification：Weigh and split kettle algae 10-15 parts, tea polyphenol liposomes 20-25 parts, fish guts oil 20-25 parts, Fish protein water
Solve thing 28-33 parts, chlorella 10-15 parts, urea 20-23 parts, ammonium nitrate 18-20 parts, calcium phosphate 15-20 parts, fertilizer 60-80
Part, phagostimulant 2-4 parts be sufficiently mixed and uniformly, be ground into the particles of 10-45 micron sizes and produce fortification bait, with 10g worms
The appetite that body feeds 4-6g bait strengthens artemia, and bait is diluted in a small amount of water into uniform full pool spilling head again during feeding, every
Once, each feeding amount is equal for 2-3 hours feeding, and reinforcing nursing 20-24 hours can feed true octopus floating larva；Strengthen bait
In rich in having the HUFA such as DHA, EPA, protein and other nutriments, can under the synergy of food calling composition and fertilizer
Promote artemia to digest and assimilate, artemia is accelerated growth, and saturated fatty acid content is extremely low in bait, and more common fish oil bait is fed
The survival rate of artemia greatly improves after supporting, and after reinforcing, the HUFA contents such as EPA and DHA of artemia significantly improve, and feed to true
After octopus floating larva, young lipid and aliphatic acid composition can be changed, improve the speed of growth, survival rate, immunity of the young with
Stress resistivity, therefore this method is a kind of effective artemia fortification method.
Preferably, fish used are perch or bighead or silver carp, this several fish guts to be thrown aside, and its fat
Acid content is higher.
Preferably, desaturase used and the mass ratio of reduced coenzyme are 6-7:1, when keeping this ratio, enzymatic
Reaction is carried out the most thorough.
Preferably, fertilizer used is cow dung or chicken manure or pig manure or soybean cake or Cottonseed Meal or rapeseed dregs, effectively
There is provided for artemia micro- in organic matter and nitrogen, phosphorus, potassium, calcium, iron, magnesium etc..
Preferably, phagostimulant used is citrus peel meal or taurine or dusty yeast or dried silkworm chrysalis meal or brewex's grains, naturally
Phagostimulant helps lend some impetus to digesting and assimilating for artemia.
Compared with prior art, the advantage of the invention is that：1）The inventive method is used in the waste material fish of fish production
It is dirty oily as raw material extraction fish guts, enzymatic reaction condition is precisely controlled, the saturated fatty acid in Application of Viscera Oil is converted into insatiable hunger
And aliphatic acid, harm of the saturated fatty acid to artemia is not only reduced, and substantially increase the unrighted acid in bait
Content, be suitable as strengthening the bait of artemia, this method is simple to operate, and materials are cheap, environmentally friendly, reached discarded object
The purpose of comprehensive utilization；2）This method can greatly improve the content of the HUFA such as DHA, EPA in artemia body, and after making reinforcing
Artemia nutrition is more comprehensively more balanced, can meet the nutritional need of true octopus floating larva culture, can change young lipid and fat
Acid composition, improve the speed of growth, survival rate, immunity of the young with stress resistivity, therefore be it is a kind of efficiently, safety it is suitable
In true octopus floating larva culture fortification formula.
Brief description of the drawings
Fig. 1 is that the survival rate of 1,2,3 enhanced time artemia different from control group of embodiment and rate of body weight gain show in the present invention
It is intended to；
Fig. 2 is the survival rate schematic diagram of the true octopus floating larva of different time sections in the present invention；
Fig. 3 is the long growth rate schematic diagram of trunk of the true octopus floating larva of different time sections in the present invention；
Fig. 4 is the content schematic diagram of unrighted acid in embodiment 1-3 fish oil in the present invention.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of artemia fortification method suitable for true octopus floating larva culture, comprise the following steps：
1）The phosphate solution of Tea Polyphenols is prepared, 300 parts of egg yolk lecithin, 58 parts of cholesterol, VE16 parts is weighed, is added to 500
In part absolute ethyl alcohol, 58 DEG C of stirring and dissolvings are heated to, are added mixed solution in Tea Polyphenols solution after dissolving completely, keeping temperature
After strong stirring 30 minutes, tea polyphenol liposomes are placed in 3 DEG C of guarantors after 55 DEG C of temperature revolving removing residual ethanols, supersound process
Deposit；Tea polyphenol liposomes envelop rate prepared by the method is high, is easy to the absorption of artemia；2）It is even to weigh 200 parts of fresh perch internal organ
Starch in beaker, add 60 parts of distilled water, stir lower warming-in-water to 30 DEG C, adjust pH to 8, continuing stirring is warming up to 45 DEG C,
25 minutes are incubated, is centrifuged while hot after being saltoutd 20 minutes after the potassium chloride stirring and dissolving of 10 parts of addition, separates to obtain crude fish oil；3）Weigh
100 parts of crude fish oil, 0.07 part of vitamin C are put in closed container and are well mixed, and reaction vessel is placed in Ultrasound Instrument, is heated to
70 DEG C and keep, be continually fed into oxygen 5 minutes, 0.3 part of desaturase, 0.05 part of reduced Coenzyme II are then added, superb
It is stand-by after being reacted 110 seconds under sound wave effect；Siphon enzymatic fish oil and with hot water wash 5 times, centrifuges, takes supernatant liquid while hot, with true
Empty drying box is drying to obtain fish guts oil；Enzymatic reaction can be seized the hydrogen of the centre position carbon-carbon double bond of saturated fatty acid by oxygen
Water is generated, so that chain saturated fatty acids lose hydrogen and are converted into corresponding cis unsaturated fatty acid, so that fish guts are oily
In rich in the polyunsaturated fatty acid such as DHA, EPA；4）Weigh split 10 parts of kettle algae, 20 parts of tea polyphenol liposomes, fish guts oil 20 parts,
28 parts of fish protein hydrolyzate, 10 parts of chlorella, 20 parts of urea, 18 parts of ammonium nitrate, 15 parts of calcium phosphate, 60 parts of peanut meal, dried silkworm chrysalis meal 2
Part is sufficiently mixed uniformly, is ground into 10 microns of particle and produces fortification bait, and the appetite that 4g bait is fed with 10g polypides is strong
Change artemia, each feeding amount is equal every 3 hours feedings once, and true octopus floating larva can be fed by strengthening to feed 21 hours；By force
Change rich in having the HUFA such as DHA, EPA, protein and other nutriments in bait, in the synergy of food calling composition and fertilizer
Under, artemia can be promoted to digest and assimilate, artemia is accelerated growth.
Embodiment 2：
A kind of artemia fortification method suitable for true octopus floating larva culture, comprise the following steps：
1）850 parts of the phosphate solution that polyphenol content is 1% is prepared, 60 DEG C is heated under strong stirring and keeps constant temperature；Weigh
320 parts of egg yolk lecithin, 60 parts of cholesterol, VE17 parts, are added in 600 parts of absolute ethyl alcohols, are heated to 60 DEG C of stirring and dissolvings, molten
Solution completely after mixed solution is added in Tea Polyphenols solution, keeping temperature strong stirring is after 45 minutes, 60 DEG C of temperature,
Rotary evaporation removes residual ethanol in 25 minutes under 0.15MPa vacuum, is ultrasonically treated after terminating tea polyphenol liposomes being placed in 3-4 DEG C
Preserve；Tea polyphenol liposomes preparation method is simple, is avoided in preparation process at the physics and chemistry containing potentially harmful substances such as ether
Reason, it is especially suitable for industrialization large-scale production；
2）210 parts of fresh silver carp internal organ homogenate are weighed into beaker, add 65 parts of distilled water, stir lower warming-in-water to 34 DEG C,
PH to 9 is adjusted, continues stirring and is warming up to 55 DEG C, be incubated 35 minutes, saltoutd 30 points after adding 14.7 parts of potassium chloride stirring and dissolving
Centrifuged while hot after clock, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；
3）Weigh 110 parts of crude fish oil, 0.08 part of vitamin C be put in closed container be well mixed, reaction vessel is placed in ultrasound
In instrument, it is heated to 73 DEG C and keeps, be continually fed into oxygen 8 minutes, then adds 0.4 part of desaturase, reduced Coenzyme II
0.06 part, reacted 140 seconds under praetersonic effect, siphon enzymatic fish oil and with hot water wash 3 times centrifuges, takes upper liquid while hot
Body, fish guts oil is drying to obtain with vacuum drying chamber；Unsaturated fatty acid content is high in this method fish oil, simple to operate, waste water,
Waste residue can be made into green high-efficient fertilizer total integration utilization, be also used as extract nucleic acid raw material it is further high-valued, reach
The purpose of waste's reclamation；
4）Weigh and split 15 parts of kettle algae, 25 parts of tea polyphenol liposomes, 25 parts of fish guts oil, 33 parts of fish protein hydrolyzate, chlorella 15
Part, 23 parts of urea, 20 parts of ammonium nitrate, 20 parts of calcium phosphate, 40 parts of cow dung, 40 parts of rapeseed dregs, 4 parts of brewex's grains are sufficiently mixed uniformly,
It is ground into 45 microns of particulate and produces fortification bait, the appetite that 6g bait is fed with 10g polypides strengthens artemia, will during feeding
Bait is diluted in a small amount of water uniform full pool spilling head again, and, each feeding amount is equal every 3 hours feedings once, strengthens and feeds
True octopus floating larva can be fed within 24 hours；The HUFA contents such as EPA in artemia body and DHA after reinforcing significantly improve, halogen
The immunocompetence enhancing of worm, nutritive value is more abundant, and the artemia after reinforcing is fed to after true octopus floating larva, can be changed
The composition of floating larva lipid and aliphatic acid, improve the speed of growth, survival rate, immunity of the young with stress resistivity, because
This this method is a kind of effective artemia fortification method.
Embodiment 3：
A kind of artemia fortification method suitable for true octopus floating larva culture, comprise the following steps：
The preparation of tea polyphenol liposomes：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.05mol/L, pH are 7.8
840 parts, 8.0 parts of Tea Polyphenols is slowly added to, 58 DEG C are heated under strong stirring and keeps constant temperature；Weigh 300 parts of egg yolk lecithin,
58 parts of cholesterol, VE17 parts, it is added in 580 parts of absolute ethyl alcohols, is heated to 60 DEG C of stirring and dissolvings, will be mixed after dissolving completely molten
Liquid is added in Tea Polyphenols solution, and keeping temperature strong stirring is after 40 minutes, the rotary evaporation under 60 DEG C of temperature, 0.12MPa vacuum
Remove residual ethanol within 25 minutes, be then ultrasonically treated, ultrasonic power 220W, frequency be 24 KHz, with ultrasonic 4 seconds intervals 4 seconds
Frequency ultrasound handle 160 seconds, tea polyphenol liposomes are placed in 3 DEG C of preservations by ultrasound after terminating；Tea Polyphenols fat prepared by the method
Plastid envelop rate is high, is easy to the absorption of artemia, and preparation method is simple, and being avoided in preparation process containing ether etc. is potential has
The physics and chemistry processing of evil material, is especially suitable for industrialization large-scale production；
Extract fish guts oil：Fresh perch class internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, in refiner
Homogenate, 210 parts of fish guts homogenate are weighed into beaker, add 60 parts of distilled water, stir lower warming-in-water to 33 DEG C, regulation pH is arrived
9, continue stirring and be warming up to 50 DEG C, be incubated 30 minutes, centrifuged while hot after being saltoutd 25 minutes after 12 parts of potassium chloride stirring and dissolvings of addition,
Separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；This method fish oil yield is high,
Simple to operate, waste water, waste residue can be made into the utilization of green high-efficient fertilizer total integration, and the raw material for being also used as extracting nucleic acid enters one
Walk high-valued, reached the purpose of waste's reclamation；
Fish oil is modified：Weigh successively vitamin E 0.16g, ethylenediamine tetra-acetic acid 0.50g, thio lauric anhydride 0.14g, L- (+)-
2,3- dihydroxypropionic acid 0.02g, acrylic resin are modified casein 2.3g, polyurethane-modified cellulose nitrate 2.5g, propane diols second
Ether 11g, it is placed in 8000r/min high-speed mixer and mixes 3 minutes to obtain coating agent, take coating agent to be evenly applied to closed
Reaction vessel inner bottom surface and inwall, dried at a temperature of 25 DEG C 25 minutes it is stand-by；Weigh 100 parts of crude fish oil, 0.08 part of vitamin C
It is put in closed container and is well mixed, reaction vessel is placed in Ultrasound Instrument, 72 DEG C is heated to and keeps, be continually fed into oxygen 6
Minute, then add 0.4 part of desaturase, 0.06 part of reduced Coenzyme II, power be 450W, frequency be the superb of 87KHz
It is stand-by after being reacted 130 seconds under sound wave effect；Siphon enzymatic fish oil and with hot water wash 4 times, centrifuges, takes supernatant liquid while hot, with true
Empty drying box is drying to obtain fish guts oil；Each film-forming components synergy, makes coating performance more stable, coating in coating agent
Quality is more uniform, and the permeability of film can be made more homogeneous, is advantageous to the release of active principle in film, and active principle can be with
Mg2+、Ca2+、Mn2+、Zn2+、Fe2+、Al3+Combined Deng two, trivalent metal ion, so as to reduce metal ion to reduced Coenzyme II
With the activity inhibition of desaturase；Particularly, when thio lauric anhydride and the mass ratio of L- (+) -2,3- dihydroxypropionic acids
For 7:When 1, the product of enzymatic reaction can be made to reduce the generation of trans unrighted acid based on cis unsaturated fatty acid
Rate, so as to reduce toxic side effect of the unrighted acid to biology；The active principle of coating release also has more in removing fish oil
The effect of cofree base, oxidative degradation of the free radical to cis unsaturated fatty acid is minimized, strengthens the steady of enzymatic preparation
It is qualitative；The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid can be seized generation water by enzymatic reaction by oxygen, so that long-chain is satisfied
Hydrogen is lost with aliphatic acid and is converted into corresponding cis unsaturated fatty acid, so that more not rich in DHA, EPA etc. in fish guts oil
Saturated fatty acid, it is highly suitable as artemia and strengthens composition；
Fortification：Weigh and split 12 parts of kettle algae, 24 parts of tea polyphenol liposomes, 24 parts of fish guts oil, 30 parts of fish protein hydrolyzate, small
10 parts of ball algae, 20 parts of urea, 20 parts of ammonium nitrate, 16 parts of calcium phosphate, 30 parts of chicken manure, 40 parts of Cottonseed Meal, 3 parts of dusty yeast are sufficiently mixed
The particle for being uniformly ground into 30 microns of sizes produces fortification bait, and the appetite that 5g bait is fed with 10g polypides strengthens artemia,
Bait is diluted in a small amount of water uniform full pool spilling head again during feeding, each feeding amount is equal every 2 hours feedings once,
Strengthen to feed 24 hours and can feed true octopus floating larva；Strengthen bait in rich in have the HUFA such as DHA, EPA, protein and other
Nutriment, under the synergy of food calling composition and fertilizer, artemia can be promoted to digest and assimilate, artemia is accelerated growth, and
And saturated fatty acid content is extremely low in bait, the survival rate of artemia greatly improves after more common fish oil bait is fed, by strengthening
Afterwards, the HUFA contents such as EPA and DHA of artemia significantly improve, and feed to after true octopus floating larva, can change young lipid and fat
Fat acid composition, improve the speed of growth, survival rate, immunity of the young with stress resistivity, therefore this method be a kind of row it
Effective artemia fortification method.
Control group：
45 micron particles are ground into by buying from the small-sized fish meal of Tianjin Chen Hui feed corporation,Ltds, with artemia body weight 1/2
Appetite feeds artemia, bait is diluted in a small amount of water into uniform full pool spilling head again during feeding, every 2 hours feedings once, often
Secondary feeding amount is equal, and true octopus floating larva is fed after feeding 22 hours.
The survival rate of artemia and the average of rate of body weight gain after in feeding different time sections, measurement is strengthened respectively, experimental data
Such as Fig. 1, as seen from Figure 1, embodiment 1-3 strengthen after artemia no matter in terms of survival rate or rate of body weight gain will better than pair
According to group, illustrate that the artemia fortification formula nutritional of the inventive method is more comprehensively more balanced, can significantly be carried after being fed to artemia
Immunity, the antibacterial resistance against diseases of high artemia, make its survival rate higher.
Embodiment 1,2,3 and artemia of the control group through strengthening are fed to true octopus floating larva, condition is fed identical
Under, take the survival rate of the true octopus floating larva of different time sections to carry out data analysis such as Fig. 2 and Fig. 3 with the long growth rate of trunk, by Fig. 2
With Fig. 3 as can be seen that in terms of true octopus floating larva survival rate and the speed of growth, embodiment 1-3 is substantially better than control group, and
With the extension of feeding time, it is possible to find the Juvenile mortality in embodiment 1-3 is gradually reducing, and growth rate is carrying steadily
Rise, illustrate that artemia is improving the speed of growth of the young, survival rate, immunity and stress resistivity etc. after fortification
It is fruitful.
Content of fatty acid measure is carried out to the fish oil in embodiment 1-3 respectively, wherein, determine insatiable hunger by determining iodine number
With the content of aliphatic acid, the specific assay method of iodine number can look into GB/T5532-2008；Trans unrighted acid is in infrared spectrum
Certain bits are equipped with absworption peak, and cis then without this characteristic, and linear return is established using the corresponding concentration of the peak area of characteristic peak
Return equation, quantitative analysis can be done to trans unrighted acid, so as to draw the content of cis unsaturated fatty acid, such as Fig. 4
It is shown.As seen from Figure 4, after purified and desaturation enzymatic reaction, the unrighted acid of fish oil contains in embodiment 1-3
Amount is far longer than control group, and in embodiment 3 for enzymatic reaction reaction vessel with the addition of coating after, not only in fish oil not
The content of saturated fatty acid is raised, and the content of wherein cis unsaturated fatty acid significantly increases, and illustrates coating not
The generation of cis unsaturated fatty acid is only promoted, and is beneficial to the desaturation of saturated fatty acid；Strengthen after feeding artemia,
The content of polyunsaturated fatty acid in artemia body can be greatly improved, makes artemia nutrition more comprehensively more balanced, can meet that true octopus floats
Swim the nutritional need of young culture, young lipid and aliphatic acid composition can be changed, improve the speed of growth of the young, survival rate,
Immunity with stress resistivity, therefore reinforcing to artemia and then to the culture important in inhibiting of true octopus floating larva.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Claims (8)
1. a kind of artemia fortification method suitable for true octopus floating larva culture, comprise the following steps：Tea polyphenol liposomes
Prepare, extraction fish guts are oily, fish oil is modified, fortification, it is characterised in that in described fish oil modification procedure, use desaturation
Enzyme carries out enzymatic modification with reduced Coenzyme II to fish oil.
2. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：The preparation process of described tea polyphenol liposomes is：Weigh the sodium dihydrogen phosphate that 0.04-0.05mol/L, pH are 7.5-7.8
With disodium hydrogen phosphate mixed solution 800-850 parts, Tea Polyphenols 8.0-8.1 parts are slowly added to, 58-60 DEG C is heated under strong stirring
And keep constant temperature；Egg yolk lecithin 300-320 parts, cholesterol 58-60 parts, VE16-17 parts are weighed, is added to 500-600 part nothings
In water-ethanol, 58-60 DEG C of stirring and dissolving is heated to, is added mixed solution in Tea Polyphenols solution after dissolving completely, keeping temperature
After strong stirring 30-45 minutes, under 55-60 DEG C of temperature, 0.1-0.15MPa vacuum rotary evaporation 20-25 minutes remove residual
Ethanol, then it is ultrasonically treated, ultrasonic power 200-230W, frequency is 23-25 KHz, is surpassed with ultrasonic 4 seconds intervals frequency of 4 seconds
Tea polyphenol liposomes are placed in 3-4 DEG C of preservation by sonication 2-3 minutes, ultrasound after terminating.
3. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：Described extraction fish guts oil step is：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, cleans and drains,
It is homogenized in refiner, weighs the homogenate of 200-210 parts fish guts into beaker, add 60-65 part distilled water, stirs lower warming-in-water
To 30-34 DEG C, pH to 8-9 is adjusted, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, adds fish guts weight 5-7%'s
Saltout after potassium chloride stirring and dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil.
4. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：Fish used are in described extraction fish guts oil step：Perch or bighead or silver carp.
5. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：Described fish oil modification procedure is：Weigh crude fish oil 100-110 parts, vitamin C 0.07-0.08 parts are put in closed container
It is well mixed, reaction vessel is placed in Ultrasound Instrument, 70-73 DEG C is heated to and keeps, is continually fed into oxygen 5-8 minutes, then
Add desaturase 0.3-0.4 parts, reduced Coenzyme II 0.05-0.06 parts, power be 400-500W, frequency 80-90KHz
The praetersonic effect lower reaction 110-140 seconds after it is stand-by；Siphon enzymatic fish oil and with hot water wash 5-3 times, centrifuges, takes while hot
Layer liquid, fish guts oil is drying to obtain with vacuum drying chamber.
6. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：Desaturase and the mass ratio of reduced Coenzyme II are 6-7 in described fish oil modification procedure:1.
7. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：Described fortification step is：Weigh and split kettle algae 10-15 parts, tea polyphenol liposomes 20-25 parts, fish guts oil 20-25
Part, chlorella 10-15 parts, fertilizer 30-40 parts, phagostimulant 2-4 parts are sufficiently mixed for uniformly, being ground into 10-45 micron sizes
Grain produces fortification bait, and the appetite that 4-6g bait is fed with 10g polypides strengthens artemia, is diluted in bait on a small quantity during feeding
Uniform full pool spilling head again in water, each feeding amount is equal every 2-3 hours feeding once, strengthens and feeds 20-24 hours
Feed true octopus floating larva.
8. a kind of artemia fortification method suitable for true octopus floating larva culture according to claim 1, its feature exist
In：Described fertilizer is cow dung or chicken manure or pig manure or soybean cake or Cottonseed Meal or rapeseed dregs, described phagostimulant are orange peel
Powder or taurine or dusty yeast or dried silkworm chrysalis meal or brewex's grains.
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