CN107535743A - One kind is suitable to true octopus floating larva culture fortification formula - Google Patents

Abstract

The invention discloses one kind to be suitable to true octopus floating larva culture fortification formula, and it is formed and its parts by weight are：Split 10 15 parts of kettle algae, 20 25 parts of tea polyphenol liposomes, 20 25 parts of fish guts oil, 10 15 parts of chlorella, 30 40 parts of fertilizer, 24 parts of phagostimulant.Have the beneficial effect that：By extracting fish guts oil and carrying out enzymatic modification, saturated fatty acid in fish oil can be converted into unrighted acid, the content of the unrighted acid such as DHA, EPA in artemia body can be greatly improved, and make the artemia nutrition after reinforcing more comprehensively more balanced, the nutritional need of true octopus floating larva culture can be met, young lipid and aliphatic acid composition can be changed, improve the speed of growth of the young, survival rate, immunity with stress resistivity, therefore be it is a kind of efficiently, safety be suitable to true octopus floating larva culture fortification formula.

Description

One kind is suitable to true octopus floating larva culture fortification formula

Technical field

The present invention relates to the aquatic products young to cultivate formulation art, is suitable to true octopus floating larva culture nutrition more particularly, to one kind Reinforcing formulation.

Technical background

Very high and steep is economic value is higher in high and steep class animal one kind, and most study, popularity highest head in the world One of sufficient class, there is wide market in Mediterranean, Latin America and Asia.It is very high and steep that there is the characteristics of being suitable to cultivation below：1） Efficiency of food conversion is high, and it can convert the 40-60% of digestion food；2）Growth rate is high, and its growth rate is about 3% daily；3） Protein content is high, accounts for the 70-90% of dry weight；4）Fish brood amount is high, and each ripe individual fish brood amount is at 100,000-50 ten thousand.Because its is wide The wealthy market space and excellent biological character, for many years numerous scholars to its ecology, physiology, behavior, breeding, growth and support Grow and conduct extensive research, excellent basis is provided to carry out very high and steep cultivation.Trunk long 1- when the very high and steep young just hatches 1.5mm, they are travelling active, and metabolic rate is high, it is necessary to a large amount of nutritious living body baits.In the stage of swimming, their shapes State change is obvious, and tentacle grows comparatively fast relative to rouge body portion, can gradually become the shape of adult from cuttlefish shape during hatching quickly Shape.Bait is a vital factor in larval culture.Researcher is made up of the fat of the young and their foods Foundation, the very high and steep food for needing to be rich in polyunsaturated fatty acid is proposed, especially to contain this 2 kinds of DHA, EPA, this is to weigh bait Expect a key factor of quality height.

Artemia is constantly subjected to the extensive weight of people as a kind of important food organisms and good experimental animal material The optimal selection eaten depending on, its naupiar larva for many tropical aquatiles first, in cultivation is put into practice, artemia be it is a kind of very Preferable true octopus floating larva bait, although studies have found that fairy shrimp palatability is good, feed positive effect, the biochemistry of artemia Composition lacks PUFA, the nutritional need of the very high and steep young can not be fully met, so as to cause the higher death of true octopus floating larva Rate and the slower speed of growth, therefore true octopus floating larva bait is strengthened just become particularly important.

At present, the technical method on true octopus floating larva culture fortification is few, and prior art such as authorizes public number For the B of CN 103141439 Chinese invention patent, the side of DHA, EPA and ARA in a kind of fortification artemia body are disclosed Method, this method carrying out fortification by will be fed to artemia after euphausia supetba dana meal ultramicro grinding, having simple to operate, cost The advantages of cheap, but its raw materials Antarctic krill has the characteristics of high protein, low fat, high mineral, it can Supplemented the nutrients well for the growth of artemia, promote increasing for its vivo acid content, and can not improved in artemia body The content of polyunsaturated fatty acid, and the saturated fatty acid in krill body can threaten to the health of artemia, therefore promote Value is little.

The content of the invention

It is an object of the invention to provide a kind of environmentally friendly, cheap to match somebody with somebody suitable for true octopus floating larva culture fortification Side.

The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：One kind is suitable to true octopus floating larva Fortification formula is cultivated, it is formed and its parts by weight are：Split kettle algae 10-15 parts, tea polyphenol liposomes 20-25 parts, fish guts Oily 20-25 parts, chlorella 10-15 parts, fertilizer 30-40 parts, phagostimulant 2-4 parts, through bait prepared by this formula rich in unsaturation Aliphatic acid, protein, amino acid and other nutriments, the artemia fed through this reinforcing bait is fed and swum children to true octopus It after body, can effectively change young lipid and aliphatic acid composition, promote larval growth development, reduce its death rate, improve immunity It is a kind of fortification formula for being safely and efficiently suitable to true octopus floating larva culture with stress reaction ability.

One kind is suitable to true octopus floating larva culture fortification formula, and its preparation method comprises the following steps：

The preparation of tea polyphenol liposomes：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate that 0.04-0.05mol/L, pH are 7.5-7.8 Mixed solution 800-850 parts, Tea Polyphenols 8.0-8.1 parts are slowly added to, 58-60 DEG C is heated under strong stirring and keeps constant temperature； Egg yolk lecithin 300-320 parts, cholesterol 58-60 parts, VE16-17 parts are weighed, is added in 500-600 part absolute ethyl alcohols, adds Heat adds mixed solution in Tea Polyphenols solution after dissolving completely, keeping temperature strong stirring 30- to 58-60 DEG C of stirring and dissolving After 45 minutes, under 55-60 DEG C of temperature, 0.1-0.15MPa vacuum rotary evaporation 20-25 minutes remove residual ethanol, Ran Houchao Sonication, ultrasonic power 200-230W, frequency are 23-25 KHz, with ultrasonic 4 seconds intervals processing of the frequency ultrasound of 4 seconds 120- 180 seconds, tea polyphenol liposomes were placed in 3-4 DEG C of preservation by ultrasound after terminating；Tea polyphenol liposomes envelop rate prepared by the method is high, It is easy to the absorption of artemia, and preparation method is simple, and the physics and chemistry containing potentially harmful substances such as ether is avoided in preparation process Processing, it is especially suitable for industrialization large-scale production；

Extract fish guts oil：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, the homogenate of 200-210 parts fish guts is weighed into beaker, adds 60-65 part distilled water, stir lower warming-in-water to 30-34 DEG C, PH to 8-9 is adjusted, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, adds fish guts weight 5-7% potassium chloride stirring Saltout after dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, to enter one Step modification is prepared；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into green high-efficient fertilizer total integration profit With, be also used as extract nucleic acid raw material it is further high-valued, reached the purpose of waste's reclamation；

Fish oil is modified：Weigh crude fish oil 100-110 parts, vitamin C 0.07-0.08 parts are put in closed container and are well mixed, general Reaction vessel is placed in Ultrasound Instrument, is heated to 70-73 DEG C and is kept, and is continually fed into oxygen 5-8 minutes, is then added desaturase 0.3-0.4 parts, reduced Coenzyme II 0.05-0.0.06 parts, in the praetersonic that power is 400-500W, frequency is 80-90KHz It is stand-by after the effect lower reaction 110-140 seconds；Siphon enzymatic fish oil and with hot water wash 5-3 times, centrifuges, takes supernatant liquid, use while hot Vacuum drying chamber is drying to obtain fish guts oil；Enzymatic reaction can be taken the hydrogen of the centre position carbon-carbon double bond of saturated fatty acid by force by oxygen Go to generate water, so that chain saturated fatty acids lose hydrogen and are converted into corresponding cis unsaturated fatty acid, so that fish guts Rich in polyunsaturated fatty acids such as DHA, EPA in oil, it is highly suitable as artemia and strengthens composition, desaturase and reduced coenzyme Mass ratio be 6-7:When 1, reactivity highest；

Fortification：Weigh and split kettle algae 10-15 parts, tea polyphenol liposomes 20-25 parts, fish guts oil 20-25 parts, chlorella 10- 15 parts, fertilizer 30-40 parts, phagostimulant 2-4 parts is sufficiently mixed and uniformly, is ground into the particles of 10-45 micron sizes to produce nutrition strong Change bait；Strengthen in bait rich in having the HUFA such as DHA, EPA, protein and other nutriments, in food calling composition and fertilizer Under synergy, artemia can be promoted to digest and assimilate, artemia is accelerated growth, and saturated fatty acid content is extremely low in bait, The survival rate of artemia greatly improves after more common fish oil bait is fed, after reinforcing, the HUFA contents such as EPA and DHA of artemia Significantly improve, feed the growth speed for young lipid and aliphatic acid composition after true octopus floating larva, can be changed, improving the young Degree, survival rate, immunity with stress resistivity, therefore this method is a kind of effective artemia fortification method.

Preferably, fish used are perch or bighead or silver carp, this several fish guts to be thrown aside, and its fat Acid content is higher.

Preferably, desaturase used and the mass ratio of reduced coenzyme are 6-7:1, when keeping this ratio, enzymatic Reaction is carried out the most thorough.

Preferably, fertilizer used is urea or ammonium nitrate or calcium phosphate or chicken manure or pig manure or soybean cake, effectively Trace element and organic matter in nitrogen, phosphorus, potassium, calcium, iron etc. are provided for artemia.

Preferably, phagostimulant used is citrus peel meal or taurine or dusty yeast or dried silkworm chrysalis meal or brewex's grains, naturally Phagostimulant helps lend some impetus to digesting and assimilating for artemia.

Compared with prior art, the advantage of the invention is that：1）The inventive method is used in the waste material fish of fish production It is dirty oily as raw material extraction fish guts, enzymatic reaction condition is precisely controlled, the saturated fatty acid in Application of Viscera Oil is converted into insatiable hunger And aliphatic acid, harm of the saturated fatty acid to artemia is not only reduced, and substantially increase the unrighted acid in bait Content, be suitable as strengthening the bait of artemia, this method is simple to operate, and materials are cheap, environmentally friendly, reached discarded object The purpose of comprehensive utilization；2）This method can greatly improve the content of the HUFA such as DHA, EPA in artemia body, and after making reinforcing Artemia nutrition is more comprehensively more balanced, can meet the nutritional need of true octopus floating larva culture, can change young lipid and fat Acid composition, improve the speed of growth, survival rate, immunity of the young with stress resistivity, therefore be it is a kind of efficiently, safety it is suitable In true octopus floating larva culture fortification formula.

Brief description of the drawings

Cis insatiable hunger when Fig. 1 is the quality ratio of different thio lauric anhydrides and L- (+) -2,3- dihydroxypropionic acids in the present invention With the schematic diagram of the content of aliphatic acid.

Embodiment

The present invention program is described further below by embodiment：

Embodiment 1：

One kind is suitable to true octopus floating larva culture fortification formula, and it is formed and its parts by weight are：Split 10 parts of kettle algae, Tea Polyphenols 20 parts of liposome, 20 parts of fish guts oil, 10 parts of chlorella, 10 parts of calcium phosphate, 20 parts of chicken manure, 2 parts of dusty yeast, its preparation method bag Include following steps：

1）The phosphate solution of Tea Polyphenols is prepared, 300 parts of egg yolk lecithin, 58 parts of cholesterol, VE16 parts is weighed, is added to 500 In part absolute ethyl alcohol, 58 DEG C of stirring and dissolvings are heated to, are added mixed solution in Tea Polyphenols solution after dissolving completely, keeping temperature After strong stirring 30 minutes, tea polyphenol liposomes are placed in 3 DEG C of guarantors after 55 DEG C of temperature revolving removing residual ethanols, supersound process Deposit；Tea polyphenol liposomes envelop rate prepared by the method is high, is easy to the absorption of artemia；2）It is even to weigh 200 parts of fresh perch internal organ Starch in beaker, add 60 parts of distilled water, stir lower warming-in-water to 30 DEG C, adjust pH to 8, continuing stirring is warming up to 45 DEG C, 25 minutes are incubated, is centrifuged while hot after being saltoutd 20 minutes after the potassium chloride stirring and dissolving of 10 parts of addition, separates to obtain crude fish oil；3）Weigh 100 parts of crude fish oil, 0.07 part of vitamin C are put in closed container and are well mixed, and reaction vessel is placed in Ultrasound Instrument, is heated to 70 DEG C and keep, be continually fed into oxygen 5 minutes, 0.3 part of desaturase, 0.05 part of reduced Coenzyme II are then added, superb It is stand-by after being reacted 110 seconds under sound wave effect；Siphon enzymatic fish oil and with hot water wash 5 times, centrifuges, takes supernatant liquid while hot, with true Empty drying box is drying to obtain fish guts oil；Enzymatic reaction can be seized the hydrogen of the centre position carbon-carbon double bond of saturated fatty acid by oxygen Water is generated, so that chain saturated fatty acids lose hydrogen and are converted into corresponding cis unsaturated fatty acid, so that fish guts are oily In rich in the polyunsaturated fatty acid such as DHA, EPA；4）Weigh split 10 parts of kettle algae, 20 parts of tea polyphenol liposomes, fish guts oil 20 parts, 10 parts of chlorella, 10 parts of calcium phosphate, 20 parts of chicken manure, 2 parts of dusty yeast are sufficiently mixed uniformly, are ground into 10 microns of particle and are produced battalion Support and strengthen bait；Strengthen rich in having the HUFA such as DHA, EPA, protein and other nutriments in bait, in food calling composition and fertilizer Under the synergy of material, artemia can be promoted to digest and assimilate, artemia is accelerated growth.

Embodiment 2：

One kind is suitable to true octopus floating larva culture fortification formula, and it is formed and its parts by weight are：Split 15 parts of kettle algae, Tea Polyphenols 25 parts of liposome, 25 parts of fish guts oil, 15 parts of chlorella, 20 parts of ammonium nitrate, 20 parts of cow dung, 2 parts of dried silkworm chrysalis meal, 2 parts of brewex's grains, Its preparation method comprises the following steps：

1）850 parts of the phosphate solution that polyphenol content is 1% is prepared, 60 DEG C is heated under strong stirring and keeps constant temperature；Weigh 320 parts of egg yolk lecithin, 60 parts of cholesterol, VE17 parts, are added in 600 parts of absolute ethyl alcohols, are heated to 60 DEG C of stirring and dissolvings, molten Solution completely after mixed solution is added in Tea Polyphenols solution, keeping temperature strong stirring is after 45 minutes, 60 DEG C of temperature, Rotary evaporation removes residual ethanol in 25 minutes under 0.15MPa vacuum, is ultrasonically treated after terminating tea polyphenol liposomes being placed in 3-4 DEG C Preserve；Tea polyphenol liposomes preparation method is simple, is avoided in preparation process at the physics and chemistry containing potentially harmful substances such as ether Reason, it is especially suitable for industrialization large-scale production；

2）210 parts of fresh silver carp internal organ homogenate are weighed into beaker, add 65 parts of distilled water, stir lower warming-in-water to 34 DEG C, PH to 9 is adjusted, continues stirring and is warming up to 55 DEG C, be incubated 35 minutes, saltoutd 30 points after adding 14.7 parts of potassium chloride stirring and dissolving Centrifuged while hot after clock, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；

3）Weigh 110 parts of crude fish oil, 0.08 part of vitamin C be put in closed container be well mixed, reaction vessel is placed in ultrasound In instrument, it is heated to 73 DEG C and keeps, be continually fed into oxygen 8 minutes, then adds 0.4 part of desaturase, reduced Coenzyme II 0.06 part, reacted 140 seconds under praetersonic effect, siphon enzymatic fish oil and with hot water wash 3 times centrifuges, takes upper liquid while hot Body, fish guts oil is drying to obtain with vacuum drying chamber；Unsaturated fatty acid content is high in this method fish oil, simple to operate, waste water, Waste residue can be made into green high-efficient fertilizer total integration utilization, be also used as extract nucleic acid raw material it is further high-valued, reach The purpose of waste's reclamation；

4）Weigh and split 15 parts of kettle algae, 25 parts of tea polyphenol liposomes, 25 parts of fish guts oil, 15 parts of chlorella, 20 parts of ammonium nitrate, ox 20 parts of excrement, 2 parts of dried silkworm chrysalis meal, 2 parts of brewex's grains are sufficiently mixed uniformly, are ground into 45 microns of particulate and are produced fortification bait；Halogen The HUFA contents such as EPA and DHA of worm significantly improve, and feed to after true octopus floating larva, can change young lipid and aliphatic acid Composition, improve the speed of growth, survival rate, immunity of the young with stress resistivity, therefore this method is a kind of effective Artemia fortification method.

Embodiment 3：

One kind is suitable to true octopus floating larva culture fortification formula, and it is formed and its parts by weight are：Split 12 parts of kettle algae, Tea Polyphenols 24 parts of liposome, 24 parts of fish guts oil, 12 parts of chlorella, 15 parts of urea, 20 parts of soybean cake, 2 parts of citrus peel meal, 1 part of taurine, Its preparation method comprises the following steps：

The preparation of tea polyphenol liposomes：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.05mol/L, pH are 7.8 840 parts, 8.0 parts of Tea Polyphenols is slowly added to, 58 DEG C are heated under strong stirring and keeps constant temperature；Weigh 300 parts of egg yolk lecithin, 58 parts of cholesterol, VE17 parts, it is added in 580 parts of absolute ethyl alcohols, is heated to 60 DEG C of stirring and dissolvings, will be mixed after dissolving completely molten Liquid is added in Tea Polyphenols solution, and keeping temperature strong stirring is after 40 minutes, the rotary evaporation under 60 DEG C of temperature, 0.12MPa vacuum Remove residual ethanol within 25 minutes, be then ultrasonically treated, ultrasonic power 220W, frequency be 24 KHz, with ultrasonic 4 seconds intervals 4 seconds Frequency ultrasound handle 160 seconds, tea polyphenol liposomes are placed in 3 DEG C of preservations by ultrasound after terminating；Tea Polyphenols fat prepared by the method Plastid envelop rate is high, is easy to the absorption of artemia, and preparation method is simple, and being avoided in preparation process containing ether etc. is potential has The physics and chemistry processing of evil material, is especially suitable for industrialization large-scale production；

Extract fish guts oil：Fresh perch class internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, in refiner Homogenate, 210 parts of fish guts homogenate are weighed into beaker, add 60 parts of distilled water, stir lower warming-in-water to 33 DEG C, regulation pH is arrived 9, continue stirring and be warming up to 50 DEG C, be incubated 30 minutes, centrifuged while hot after being saltoutd 25 minutes after 12 parts of potassium chloride stirring and dissolvings of addition, Separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；This method fish oil yield is high, Simple to operate, waste water, waste residue can be made into the utilization of green high-efficient fertilizer total integration, and the raw material for being also used as extracting nucleic acid enters one Walk high-valued, reached the purpose of waste's reclamation；

Fish oil is modified：Sodium ethylene diamine tetracetate 0.50g, vitamin C 0.20g, thio lauric anhydride 0.07g, L- are weighed successively (+) -2,3- dihydroxypropionic acids 0.01g, acrylate modified butadiene resin 1.8g, polyurethane-modified cellulose nitrate 2.5g, third Glycol ether 13.1g, it is placed in 7000r/min high-speed mixer and mixes 4 minutes to obtain coating agent, take coating agent uniformly to apply Be overlying on closed reaction vessel inner bottom surface and inwall, dried at a temperature of 30 DEG C 15 minutes it is stand-by；Weigh 100 parts of crude fish oil, dimension life Plain C0.08 parts are put in closed container and are well mixed, and reaction vessel is placed in Ultrasound Instrument, 72 DEG C is heated to and keeps, and are continued Be passed through oxygen 6 minutes, then add 0.4 part of desaturase, 0.06 part of reduced Coenzyme II, power be 450W, frequency be It is stand-by after being reacted 130 seconds under 87KHz praetersonic effect；Siphon enzymatic fish oil and with hot water wash 4 times, centrifuges, takes while hot Layer liquid, fish guts oil is drying to obtain with vacuum drying chamber；Enzymatic reaction can be by the centre position carbon-carbon double bond of saturated fatty acid Hydrogen generation water is seized by oxygen so that chain saturated fatty acids lose hydrogen and are converted into corresponding cis unsaturated fatty acid, from And make to be highly suitable as artemia rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil and strengthen composition；

Fortification：Weigh and split 12 parts of kettle algae, 24 parts of tea polyphenol liposomes, 24 parts of fish guts oil, 12 parts of chlorella, urea 15 Part, 20 parts of soybean cake, 2 parts of citrus peel meal, 1 part of taurine, which are sufficiently mixed, uniformly produces fortification bait, will strengthen bait powder It is broken into the particle of 30 microns of sizes；Strengthen in bait rich in having the HUFA such as DHA, EPA, protein and other nutriments, luring Under the synergy for eating composition and fertilizer, artemia can be promoted to digest and assimilate, artemia is accelerated growth, and saturated fat in bait Fat acid content is extremely low, and the survival rate of artemia greatly improves after more common fish oil bait is fed, after reinforcing, the EPA of artemia and The HUFA contents such as DHA significantly improve, and feed to that after true octopus floating larva, can change young lipid and aliphatic acid composition, improve The speed of growth of the young, survival rate, immunity with stress resistivity, therefore this method is a kind of effective artemia nutrition Intensifying method.

Embodiment 4：

Extract fish guts oil：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, the homogenate of 200-210 parts fish guts is weighed into beaker, adds 60-65 part distilled water, stir lower warming-in-water to 30-34 DEG C, PH to 8-9 is adjusted, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, adds fish guts weight 5-7% potassium chloride stirring Saltout after dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, to enter one Step modification is prepared；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into green high-efficient fertilizer total integration profit With, be also used as extract nucleic acid raw material it is further high-valued, reached the purpose of waste's reclamation；

It is coating modified：Sodium ethylene diamine tetracetate 0.50g, vitamin C 0.20g, acrylate modified butadiene resin are weighed successively 1.8g, polyurethane-modified cellulose nitrate 2.5g, propylene-glycol ethyl ether 13.1g, L- (+) -2,3- dihydroxypropionic acids, thio laurate Acid anhydride, the quality of L- (+) -2,3- dihydroxypropionic acids are 0.01g, the quality of thio lauric anhydride is 0.050g, 0.051g, 0.053g、……、0.079g、0.080g；It is placed in 10000r/min high-speed mixer and mixes 2.5 minutes to obtain coating agent, Take coating agent to be evenly applied to closed reaction vessel inner bottom surface and inwall, dried at a temperature of 25 DEG C 25 minutes it is stand-by；

Fish oil is modified：Weigh 110 parts of crude fish oil, 0.08 part of vitamin C be put in closed container be well mixed, reaction vessel is put In Ultrasound Instrument, it is heated to 72 DEG C and keeps, be continually fed into oxygen 6 minutes, it is auxiliary then adds 0.35 part of desaturase, reduced form II 0.055 parts of enzyme, it is stand-by after being reacted 125 seconds under the praetersonic effect that power is 480W, frequency is 88KHz；Siphon enzymatic Fish oil and with hot water wash 5-3 times, centrifuges, takes supernatant liquid, fish guts oil is drying to obtain with vacuum drying chamber while hot；Enzymatic reaction The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid can be seized generation water by oxygen, so that chain saturated fatty acids lose hydrogen Corresponding cis unsaturated fatty acid is converted into, so that being rich in the polyunsaturated fatty acids such as DHA, EPA in fish guts oil, very It is suitable as artemia and strengthens composition, the mass ratio of desaturase and reduced coenzyme is 6-7:When 1, reactivity highest；

Reinforcing raising is carried out to artemia with the bait of the large krill of fish market purchase as a control group, by large krill dries pulverizing Reinforcing raising is carried out to artemia respectively for the reinforcing bait in 30 microns of particle, with embodiment 1-3；6g is fed with 10g polypides The appetite of feed strengthens artemia, bait is diluted in a small amount of water into uniform full pool spilling head again during feeding, every 2 hours feedings one Secondary, each feeding amount is equal, and true octopus floating larva can be fed by strengthening to feed 8 hours；Line number is entered to strengthening result with ocular estimate It is as shown in table 1 according to analysis：

The embodiment 1-3 of table 1. and control group artemia fortification experimental data

As it can be seen from table 1 the data in artemia survival rate and the aspect embodiment 1-3 of rate of body weight gain two are all significantly better than control group, The reinforcing food nutrition that illustrates this experimental method is balanced, reasonable mixture ratio, after strengthening artemia, can strengthen artemia immunocompetence, Disease-resistant antibacterial ability, the artemia death rate is reduced, it can also be promoted to digest and assimilate, improve its growth rate, therefore be one The efficient artemia fortification formula of kind；Simultaneously it can also be seen that in it with the addition of the embodiment 3 of modified coating, it is survived Rate and rate of body weight gain are all higher than the embodiment 1,2 of not added modified coating, illustrate that modified coating is modified to fish oil and serve actively Effect.

Content of fatty acid measure is carried out to the fish oil in embodiment 1-3 respectively, wherein according to the iodine in GB/T5532-2008 Values determination method determines the content of unrighted acid, is equipped with according to certain bits of the trans unrighted acid in infrared spectrum The content of absworption peak and the cis then characteristic measurement cis unsaturated fatty acid without this characteristic, spectral instrument are that Fourier transform is red External spectrum instrument (Spectrum 100), experimental result is as shown in table 2：

Unrighted acid and cis unsaturated fatty acid content in the fish oil of 2. embodiment of table 1,2,3

	Embodiment 1	Embodiment 2	Embodiment 3
				Unsaturated fatty acid content/%	36.5%	38.2%	55.9%
Cis unsaturated fatty acid content/%	4.9%	5.6%	43.5%

As can be seen from Table 2, the content of the unrighted acid of fish oil is all significantly higher than in embodiment 1-3 after enzymatic modification Common commercially available fish oil 25-30% unsaturated fatty acid content, illustrates that enzymatic reaction is converted into fractional saturation fatty acid desaturation Unrighted acid, contributes to the enrichment of PUFA in artemia body, so as to help digesting and assimilating for true octopus floating larva；Especially Ground, it can be seen that, coating agent can greatly improve the content of cis unsaturated fatty acid in fish oil in embodiment 3, and What is stabilized it is present in fish oil, and this has deep meaning for the nutrient laden of artemia.

In example 4, when thio lauric anhydride and L- (+) -2,3- dihydroxypropionic acids are between 5:1-8:In 1 section When, keep other the same terms to carry out the modification of fish oil, the cis unsaturated lipid in each Ratio Experiments is determined with infrared spectrum method Fat acid content, as shown in Figure 1.As seen from Figure 1, thio lauric anhydride and L- (+) -2,3- dihydroxypropionic acids in modified coating Mass ratio the content of cis unsaturated fatty acid in enzymatic preparation is had a great influence, wherein when thio lauric anhydride and L- (+)- The mass ratio of 2,3- dihydroxypropionic acids is between 6.0:1-7.0:The content of cis unsaturated fatty acid is higher when 1, especially, works as sulphur It is 6.7 for the mass ratio of lauric anhydride and L- (+) -2,3- dihydroxypropionic acids:When 1 in product cis unsaturated fatty acid content 50%, and the as can be seen from the figure content of cis unsaturated fatty acid and thio lauric anhydride and L- in product are exceeded The not linear correlation of mass ratio of (+) -2,3- dihydroxypropionic acids, its oligodynamics mechanism are still not clear.

Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.

Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.

Claims (9)

1. one kind is suitable to true octopus floating larva culture fortification formula, it is formed and its parts by weight are：Split kettle algae 10-15 parts, Tea polyphenol liposomes 20-25 parts, fish guts oil 20-25 parts, chlorella 10-15 parts, fertilizer 30-40 parts, phagostimulant 2-4 parts.

2. one kind according to claim 1 is suitable to true octopus floating larva culture fortification formula, it is characterised in that：It is described Fertilizer be urea or ammonium nitrate or calcium phosphate or chicken manure or pig manure or soybean cake, described phagostimulant is citrus peel meal or ox sulphur Acid or dusty yeast or dried silkworm chrysalis meal or brewex's grains.

3. one kind according to claim 1 is suitable to true octopus floating larva culture fortification formula, it is characterised in that：It is made Preparation Method comprises the following steps：

1）The preparation of tea polyphenol liposomes：Tea polyphenol liposomes are prepared by raw material of egg yolk lecithin；

2）Extract fish guts oil：With potassium method extraction fish guts oil；

3）Fish oil is modified：Enzymatic reaction is modified to fish oil；

4）Fortification：It is equipped with fortification bait.

4. a kind of preparation method suitable for true octopus floating larva culture fortification formula according to claim 3, it is special Sign is：The preparation process of described tea polyphenol liposomes is：Weigh the di(2-ethylhexyl)phosphate that 0.04-0.05mol/L, pH are 7.5-7.8 Hydrogen sodium and disodium hydrogen phosphate mixed solution 800-850 parts, are slowly added to Tea Polyphenols 8.0-8.1 parts, 58- are heated under strong stirring 60 DEG C and keep constant temperature；Egg yolk lecithin 300-320 parts, cholesterol 58-60 parts, VE16-17 parts are weighed, is added to 500-600 In part absolute ethyl alcohol, 58-60 DEG C of stirring and dissolving is heated to, adds mixed solution in Tea Polyphenols solution after dissolving completely, is kept After temperature strong stirring 30-45 minutes, under 55-60 DEG C of temperature, 0.1-0.15MPa vacuum rotary evaporation 20-25 minutes remove Residual ethanol, then it is ultrasonically treated, ultrasonic power 200-230W, frequency is 23-25 KHz, with ultrasonic 4 seconds intervals frequency of 4 seconds Rate is ultrasonically treated 2-3 minutes, and tea polyphenol liposomes are placed in 3-4 DEG C of preservation by ultrasound after terminating.

5. a kind of preparation method suitable for true octopus floating larva culture fortification formula according to claim 3, it is special Sign is：Described extraction fish guts oil step is：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, clean drip It is dry, it is homogenized in refiner, weighs the homogenate of 200-210 parts fish guts into beaker, add 60-65 part distilled water, stirs lower water Bath is warming up to 30-34 DEG C, adjusts pH to 8-9, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, adds fish guts weight Saltout after 5-7% potassium chloride stirring and dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil.

6. a kind of preparation method suitable for true octopus floating larva culture fortification formula according to claim 3, it is special Sign is：Fish used are in described extraction fish guts oil step：Perch or bighead or silver carp.

7. a kind of preparation method suitable for true octopus floating larva culture fortification formula according to claim 3, it is special Sign is：Described fish oil modification procedure is：Weigh crude fish oil 100-110 parts, vitamin C 0.07-0.08 parts are put in closed appearance It is well mixed in device, reaction vessel is placed in Ultrasound Instrument, 70-73 DEG C is heated to and keeps, is continually fed into oxygen 5-8 minutes, Then desaturase 0.3-0.4 parts, reduced Coenzyme II 0.05-0.06 parts are added, power be 400-500W, frequency 80- It is stand-by after the 90KHz praetersonic effect lower reaction 110-140 seconds；Siphon enzymatic fish oil and with hot water wash 5-3 times, while hot from The heart, supernatant liquid is taken, fish guts oil is drying to obtain with vacuum drying chamber.

8. a kind of preparation method suitable for true octopus floating larva culture fortification formula according to claim 3, it is special Sign is：Desaturase and the mass ratio of reduced Coenzyme II are 6-7 in described fish oil modification procedure:1.

9. a kind of preparation method suitable for true octopus floating larva culture fortification formula according to claim 3, it is special Sign is：Described fortification step is：Weigh and split kettle algae 10-15 parts, tea polyphenol liposomes 20-25 parts, fish guts oil 20- 25 parts, chlorella 10-15 parts, fertilizer 30-40 parts, phagostimulant 2-4 parts be sufficiently mixed uniformly, be ground into 10-45 micron sizes Particle produces fortification bait.