CN101081015A - Method for producing artemia nauplius from artemia sporangiocyst - Google Patents
Method for producing artemia nauplius from artemia sporangiocyst Download PDFInfo
- Publication number
- CN101081015A CN101081015A CNA2007101374098A CN200710137409A CN101081015A CN 101081015 A CN101081015 A CN 101081015A CN A2007101374098 A CNA2007101374098 A CN A2007101374098A CN 200710137409 A CN200710137409 A CN 200710137409A CN 101081015 A CN101081015 A CN 101081015A
- Authority
- CN
- China
- Prior art keywords
- artemia
- additive
- sporocyst
- culture medium
- artemia sporocyst
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001247197 Cephalocarida Species 0.000 title claims description 77
- 241000133262 Nauplius Species 0.000 title abstract description 8
- 238000004519 manufacturing process Methods 0.000 title description 3
- 239000000654 additive Substances 0.000 claims abstract description 31
- 230000000996 additive effect Effects 0.000 claims abstract description 30
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 24
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 5
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 5
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 claims abstract description 5
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000001263 FEMA 3042 Substances 0.000 claims abstract description 3
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims abstract description 3
- 239000000284 extract Substances 0.000 claims abstract description 3
- 229920002258 tannic acid Polymers 0.000 claims abstract description 3
- 235000015523 tannic acid Nutrition 0.000 claims abstract description 3
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 claims abstract description 3
- 229940033123 tannic acid Drugs 0.000 claims abstract description 3
- 238000011534 incubation Methods 0.000 claims description 34
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims description 20
- 230000012447 hatching Effects 0.000 claims description 17
- 229940074391 gallic acid Drugs 0.000 claims description 10
- 235000004515 gallic acid Nutrition 0.000 claims description 10
- 229940087603 grape seed extract Drugs 0.000 claims description 4
- 235000002532 grape seed extract Nutrition 0.000 claims description 4
- 239000005416 organic matter Substances 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 239000013535 sea water Substances 0.000 claims description 4
- 239000001717 vitis vinifera seed extract Substances 0.000 claims description 4
- 102000004895 Lipoproteins Human genes 0.000 claims description 2
- 108090001030 Lipoproteins Proteins 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 229920001864 tannin Polymers 0.000 claims description 2
- 239000001648 tannin Substances 0.000 claims description 2
- 235000018553 tannin Nutrition 0.000 claims description 2
- 241000238582 Artemia Species 0.000 abstract description 8
- 206010011732 Cyst Diseases 0.000 abstract description 7
- 208000031513 cyst Diseases 0.000 abstract description 7
- 230000005058 diapause Effects 0.000 abstract description 6
- 239000012267 brine Substances 0.000 abstract description 3
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 abstract description 3
- 241000238426 Anostraca Species 0.000 abstract 6
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 abstract 3
- 230000003311 flocculating effect Effects 0.000 abstract 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 abstract 1
- 230000009182 swimming Effects 0.000 abstract 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 14
- 229960002163 hydrogen peroxide Drugs 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 230000000284 resting effect Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 2
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 description 2
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 2
- LHJQIRIGXXHNLA-UHFFFAOYSA-N calcium peroxide Chemical compound [Ca+2].[O-][O-] LHJQIRIGXXHNLA-UHFFFAOYSA-N 0.000 description 2
- 229920002414 procyanidin Polymers 0.000 description 2
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 239000004343 Calcium peroxide Substances 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- SPAGIJMPHSUYSE-UHFFFAOYSA-N Magnesium peroxide Chemical compound [Mg+2].[O-][O-] SPAGIJMPHSUYSE-UHFFFAOYSA-N 0.000 description 1
- 235000019402 calcium peroxide Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- -1 perborate Chemical class 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- MWNQXXOSWHCCOZ-UHFFFAOYSA-L sodium;oxido carbonate Chemical compound [Na+].[O-]OC([O-])=O MWNQXXOSWHCCOZ-UHFFFAOYSA-L 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The method of incubating brine shrimp cyst to produce free swimming nauplius brine shrimp larva is to setting brine shrimp cyst and the additive in the weight ratio of 100 to 0.5-4 simultaneously into incubating culture medium. The additive is organic benzene containing one or several o-hydroxy radicals, such as pyrogallic acid, methyl pyrogallate, tannic acid, tanning extract, ea polyphenol, etc. The additive can damage the diapause mechanism of brine shrimp cyst to raise the brine shrimp cyst incubating within 24 hr to 90 %, and has flocculating effect to separate nauplius brine shrimp larva easily from un-incubated brine shrimp cyst. The present invention expands the application range of incubating culture medium and is especially suitable for incubating treatment of brine shrimp cyst the inland brine lake produces.
Description
(1) technical field
The present invention relates to a kind of production method that is used for aquaculture live body feed, particularly a kind of method of producing free-swimming artemia nauplii from artemia sporocyst.
(2) background technology
Artemia nauplii generally is used as the live body feed of aquaculture, especially as ocean fish, the early stage live body feed of extra large shrimp larval stage.Generally do not have the such live body feed of artemia nauplii on the market, have only artemia sporocyst, nauplius hatches from artemia sporocyst.The hatching of artemia sporocyst can be by finishing in incubation culture medium.Artemia sporocyst is through abundant dehydration and under the dry environment of the unglazed photograph of low temperature anaerobic, can survive for a long time even reaches several years.The storage capacity of overlength and short time make it become the feed resource of live body the most easily of aquaculture as producing the characteristic of free-swimming nauplius in 24 hours.
Artemia sporocyst is collected from natural world such as salt pan, seashore and inland brine lake.In the artemia sporocyst of these collections, there be the dormancy sporangiocyst and the diapause sporangiocyst of different proportion.Artemia sporocyst is purified to remove disintegrating slag, washing to remove salinity, to carry out drying processing and cold house's preservation at last, make it be in resting state.The quality of artemia sporocyst is judged by evaluate parameter, wherein but the hatchability of artemia sporocyst is subjected to people's attention most, because it has directly shown under artificial incubation conditions, the quantity of the live body feed (nauplius) that can obtain from the artemia sporocyst of some.But the hatchability of artemia sporocyst uses incubation rate (H%) to represent usually, promptly refers to the number of the free-swimming nauplius that hatches from 100 complete artemia sporocysts.Be in the artemia sporocyst under diapause or the resting state, can not continue to hatch into free-swimming nauplius, unless be under the condition that promotes hatching, therefore the hatching of artemia sporocyst need be finished in incubation culture medium, incubation culture medium is generally treated clean sea water, and its abiotic parameter comprises: salinity, pH value, oxygen concentration, water temperature and illumination.The present parameter area that adopts usually of each production unit: salinity be 15～35g dissolving salt/liter; PH＞8; Oxygen concentration＞5mg/L; 25～28 ℃ of water temperatures, the intensity of illumination on the water surface should be not less than 2000lux.
The hatching of artemia sporocyst is an extremely complicated process, generally includes three phases: preceding phase growth, umbrella stage and actual incubation period.But the factor that influences the artemia sporocyst hatching process is a lot, the hatching process that carries out according to above-mentioned given incubation condition often can not reach the hatching effect of expection, therefore need take special diapause method for deactivating, for example prolong dry artemia sporocyst the freezing resting period, repeat to absorb water-dewatering cycle, increase as additive the hatching amount of artemia sporocyst etc. with some chemical substance.Usually use hydrogenperoxide steam generator to handle artemia sporocyst at present to improve the incubation rate of artemia sporocyst.The shortcoming that adopts hydrogenperoxide steam generator to handle artemia sporocyst is: 1) using preceding is the artemia sporocyst of different batches at different halogen worm strains systems or same strain, need carry out a large amount of tests and determine the optimum parameter, and in order to obtain desirable hatching effect, also must be strict controlled under this parameter during operation and carry out, this brings great inconvenience to the user; 2) need in the implementation process artemia sporocyst after the hydration is transferred in hydrogenperoxide steam generator and the corresponding incubation culture medium, and the carrying out of these steps is very strict to the requirement of implementing the time, makes extremely very complicated of operating procedure; 3) artemia sporocyst is through behind the hydrogenperoxide steam generator, must wash with water timely removing remaining hydrogen peroxide, even need carry out neutralisation treatment, certainly will increase processing cost.Another processing method that adopts hydrogen peroxide is solid chemical compound such as perborate, percarbonate, peromag or the calper calcium peroxide etc. that direct interpolation can produce hydrogen peroxide in incubation culture medium, but the disadvantage that adopts this method is bigger to the pH value change of incubation culture medium, and too high pH value might cause the death of artemia nauplii.
(3) summary of the invention
The objective of the invention is to overcome above-mentioned shortcoming of the prior art, a kind of high and convenient-to-running method of producing free-swimming artemia nauplii from artemia sporocyst of efficient of hatching is provided.
Technical scheme of the present invention:
A kind of method of producing free-swimming artemia nauplii from artemia sporocyst, the hatching of artemia sporocyst is carried out in incubation culture medium, incubation culture medium is treated clean sea water, it is characterized in that: artemia sporocyst and additive are joined in the incubation culture medium simultaneously, and additive is the organic matter that contains one or more o-hydroxies.
A kind of above-mentioned method of producing free-swimming artemia nauplii from artemia sporocyst is characterized in that: additive is a kind of, two or more the combination in pyrogallic acid, gallic acid, gallic acid formicester, gallic acid second fat, gallic acid third lipoprotein, tannic acid, tannin extract, Tea Polyphenols, apple polyphenol, Pine Bark, the grape seed extract.
A kind of above-mentioned method of producing free-swimming artemia nauplii from artemia sporocyst is characterized in that: the weight ratio of artemia sporocyst and additive is 100: 0.5～4.
A kind of above-mentioned method of producing free-swimming artemia nauplii from artemia sporocyst is characterized in that: the total amount of artemia sporocyst and additive and the weight ratio of incubation culture medium are 0.2～0.5: 100.
A kind of above-mentioned method of producing free-swimming artemia nauplii from artemia sporocyst, it is characterized in that: additive directly is wrapped on the artemia sporocyst.
A kind of above-mentioned method of producing free-swimming artemia nauplii from artemia sporocyst is characterized in that: additive is with form packing or separately independent packing after artemia sporocyst mixes of pressed powder.
Advantage of the present invention is: since in incubation culture medium, added contain one or more o-hydroxies organic matter as additive, can destroy the diapause mechanism of artemia sporocyst, impel the diapause sporangiocyst during incubation to produce free-swimming artemia nauplii, incubation rate in the artemia sporocyst 24 hours is reached about 90%, and improved hatching efficient; Have the flocculant effect owing to contain the organic matter of one or more o-hydroxies, artemia nauplii is separated easily with the artemia sporocyst of not hatching fully, running cost is low, and is easy to use; Reduced in the hatching process the parameter request of cyst density, salinity or water temperature, promptly enlarged the scope of application of incubation culture medium, be specially adapted to the artemia sporocyst that inland brine lake is produced is hatched processing.
(4) embodiment
Artemia sporocyst adopts and originates in the worm's ovum of Russian inland lake in the present embodiment, and removes impurity, salinity, ghost and dry the processing; Incubation culture medium is 1 liter of treated clean sea water, and its abiotic parameter is: the intensity of illumination on salinity 20g dissolving salt/L, pH value 8.2, oxygen concentration 3mg/L, 28 ℃ of water temperatures, the water surface is not less than 2000lux; Select dissimilar additives for use; Artemia sporocyst and additive are added in the incubation culture medium hatching 24 hours simultaneously, detect the incubation rate of artemia sporocyst.
Embodiment 1: additive is pyrogallic acid (technical pure).Add 0.04 gram pyrogallic acid in 4 gram artemia sporocysts, the weight ratio of artemia sporocyst and additive is 100: 1, and the total amount of artemia sporocyst and additive and the weight ratio of incubation culture medium are 0.404: 100, and the incubation rate that records artemia sporocyst is 89%.
Embodiment 2: additive is Pine Bark (procyanidin content 〉=95%, Ningbo City's herbal pharmaceutical factory produce).Add 0.05 gram Pine Bark in 2 gram artemia sporocysts, the weight ratio of artemia sporocyst and additive is 100: 2.5, and the total amount of artemia sporocyst and additive and the weight ratio of incubation culture medium are 0.205: 100, and the incubation rate that records artemia sporocyst is 92%.
Embodiment 3: additive is the mixture (technical pure) of grape seed extract (procyanidin content 〉=95%, Tianjin spike natural products research and development Co., Ltd produce) and gallic acid.In 4 gram artemia sporocysts, add 0.08 gram grape seed extract and 0.01 gram gallic acid, the weight ratio of artemia sporocyst and additive is 100: 2.25, the total amount of artemia sporocyst and additive and the weight ratio of incubation culture medium are 0.409: 100, and the incubation rate that records artemia sporocyst is 90%.
The foregoing description testing result is as can be seen: artemia sporocyst and additive are joined simultaneously carry out hatching in 24 hours in the incubation culture medium, incubation rate all reaches about 90%, and effect is remarkable; This method simple and feasible, result of use is good.
Claims (6)
1. method of producing free-swimming artemia nauplii from artemia sporocyst, the hatching of artemia sporocyst is carried out in incubation culture medium, incubation culture medium is treated clean sea water, it is characterized in that: artemia sporocyst and additive are joined in the incubation culture medium simultaneously, and additive is the organic matter that contains one or more o-hydroxies.
2. method according to claim 1 is characterized in that: additive is a kind of, two or more the combination in pyrogallic acid, gallic acid, gallic acid formicester, gallic acid second fat, gallic acid third lipoprotein, tannic acid, tannin extract, Tea Polyphenols, apple polyphenol, Pine Bark, the grape seed extract.
3. method according to claim 1 is characterized in that: the weight ratio of artemia sporocyst and additive is 100: 0.5～4.
4. method according to claim 1 is characterized in that: the total amount of artemia sporocyst and additive and the weight ratio of incubation culture medium are 0.2～0.5: 100.
5. method according to claim 1, it is characterized in that: additive directly is wrapped on the artemia sporocyst.
6. method according to claim 1 is characterized in that: additive is with form packing or separately independent packing after artemia sporocyst mixes of pressed powder.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101374098A CN101081015A (en) | 2006-08-18 | 2007-07-11 | Method for producing artemia nauplius from artemia sporangiocyst |
| PCT/CN2007/002501 WO2008022569A1 (en) | 2006-08-18 | 2007-08-20 | Method to hatch free swimming nauplii from artemia cysts |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610015369 | 2006-08-18 | ||
| CN200610015369.5 | 2006-08-18 | ||
| CNA2007101374098A CN101081015A (en) | 2006-08-18 | 2007-07-11 | Method for producing artemia nauplius from artemia sporangiocyst |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101081015A true CN101081015A (en) | 2007-12-05 |
Family
ID=38910973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101374098A Pending CN101081015A (en) | 2006-08-18 | 2007-07-11 | Method for producing artemia nauplius from artemia sporangiocyst |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN101081015A (en) |
| WO (1) | WO2008022569A1 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106322A (en) * | 2011-01-28 | 2011-06-29 | 深圳市龙科源水产养殖有限公司 | System and method for closing culture of artemia |
| CN102027891B (en) * | 2009-09-29 | 2013-03-13 | 天津市佳音生物饲料有限公司 | Method for increasing hatchability of artemia cysts |
| CN103651261A (en) * | 2012-09-21 | 2014-03-26 | 上海海洋大学 | Method for incubating brine shrimps through biological flocculating constituent suspension liquid |
| CN104273098A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for hatching under-diapause brine shrimp eggs in ultraviolet light |
| CN107455615A (en) * | 2017-07-21 | 2017-12-12 | 浙江海洋大学 | A kind of artemia fortification method suitable for true octopus floating larva culture |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563860B (en) * | 2013-11-22 | 2015-03-11 | 天津海友佳音生物科技股份有限公司 | Method for increasing hatching rate of diapause artemia eggs in weak light environment |
| CN106719168A (en) * | 2016-12-14 | 2017-05-31 | 中国电建集团贵阳勘测设计研究院有限公司 | A kind of fish multiplication pouring station intensive artemia hatching apparatus and its application method |
| CN111789068A (en) * | 2020-07-16 | 2020-10-20 | 滨州市胜英水产有限公司 | Fairy shrimp egg hatching equipment and hatching method thereof |
| CN112167131B (en) * | 2020-09-30 | 2023-03-10 | 天津科技大学 | Halobacterium rubrum and rhodobacter salina strain and application of strengthened artemia thereof in aquatic seedling raising or aquaculture |
| CN113615610A (en) * | 2021-07-29 | 2021-11-09 | 天津海友佳音生物科技股份有限公司 | Hatching method for improving hatchability of artemia cysts |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100414818B1 (en) * | 1994-10-21 | 2004-03-30 | 인베 아쿠아컬쳐 엔. 브이. | How to reduce bacterial contamination in aqueous culture solutions |
| TWI226818B (en) * | 2000-10-05 | 2005-01-21 | Inve Technologies N V | Method for producing free swimming Artemia nauplii and packaged cysts for use in that method |
| WO2004091307A2 (en) * | 2003-04-08 | 2004-10-28 | Advanced Bionutriton Corporation | Feed additives against diseasse infection in terrestrial and aquatic animals |
| EP1767101A1 (en) * | 2005-09-26 | 2007-03-28 | Inve Technologies N.V. | Method to enhance hatching percentage of Artemia diapauzing cysts |
-
2007
- 2007-07-11 CN CNA2007101374098A patent/CN101081015A/en active Pending
- 2007-08-20 WO PCT/CN2007/002501 patent/WO2008022569A1/en active Application Filing
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102027891B (en) * | 2009-09-29 | 2013-03-13 | 天津市佳音生物饲料有限公司 | Method for increasing hatchability of artemia cysts |
| CN102106322A (en) * | 2011-01-28 | 2011-06-29 | 深圳市龙科源水产养殖有限公司 | System and method for closing culture of artemia |
| CN103651261A (en) * | 2012-09-21 | 2014-03-26 | 上海海洋大学 | Method for incubating brine shrimps through biological flocculating constituent suspension liquid |
| CN103651261B (en) * | 2012-09-21 | 2016-04-27 | 上海海洋大学 | Bioflocculation liquid suspension is utilized to hatch the method for halogen worm |
| CN104273098A (en) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | Method for hatching under-diapause brine shrimp eggs in ultraviolet light |
| CN104273098B (en) * | 2014-10-21 | 2016-08-24 | 天津海友佳音生物科技股份有限公司 | A kind of method hatching diapause artemia cysts under ultraviolet light |
| CN107455615A (en) * | 2017-07-21 | 2017-12-12 | 浙江海洋大学 | A kind of artemia fortification method suitable for true octopus floating larva culture |
| CN107455615B (en) * | 2017-07-21 | 2020-05-22 | 浙江海洋大学 | Artemia nutrition strengthening method suitable for culturing octopus ocellatus planktonic larvae |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008022569A1 (en) | 2008-02-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101081015A (en) | Method for producing artemia nauplius from artemia sporangiocyst | |
| CN102524196B (en) | Method for improving hatching rate of artemia cysts | |
| CN102040284B (en) | Large benthos and submerged plant combined eutrophication control method | |
| CN102630639A (en) | Shelling method of artemia cyst | |
| CN103563860B (en) | Method for increasing hatching rate of diapause artemia eggs in weak light environment | |
| CN110432381A (en) | A kind of insect source property shrimp seedling special health feed | |
| EP1767101A1 (en) | Method to enhance hatching percentage of Artemia diapauzing cysts | |
| CN101779595A (en) | Method for implanting and cultivating Gracilaria bursa-pastoris in north pond | |
| Ravuru et al. | Effect of density on growth and production of Litopenaeus vannamei of brackish water culture system in summer season with artificial diet in Prakasam District, India | |
| Peduzzi | Dietary Preferences and Carbon Absorption by two Grazing Gastropods, G/bbula umbilicaris (LINNÉ) and Jujubinus striatus (LINNÉ) | |
| CN103782929A (en) | Method for colonizing and releasing seawater mullets in public freshwater area | |
| CN105746446B (en) | A kind of decladding method of artemia eggs | |
| KR101641732B1 (en) | Food processing methods of Aurelia aurita | |
| CN103947818B (en) | A kind of preparation method of squid active peptide | |
| Viitasalo | Seasonal succession and long-term changes of mesozooplankton in the northern Baltic Sea. | |
| RU2722034C1 (en) | Method for producing collagen-protein complex from animal skins | |
| KR100399252B1 (en) | A Handling Methods of the Kanari Waste | |
| DK2473611T3 (en) | System for producing raw material for biogas production | |
| Munuswamy et al. | Culturing the fairy shrimp Streptocephalus dichotomus Baird using livestock waste–a reclamation study | |
| CN109053502A (en) | A method of taurine is extracted using marine product leftover bits and pieces | |
| CN101928404B (en) | Preparation of imprinted crosslinked chitosan resin and application thereof in removal of cadmium from pinctada martensii homogenate | |
| JP2008000738A (en) | Recycling utilization technique for seashore thrown seaweed and business system | |
| CN202849399U (en) | Device used for crushing algae cell | |
| Hessen et al. | Nutrient release from crayfish, and its potential impact on primary production in lakes | |
| Litvinenko et al. | Features of Artemia aquaculture technology in Russia, prospects for its use in other temperate and tropical climates |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20071205 |