CN202849399U - Device used for crushing algae cell - Google Patents
Device used for crushing algae cell Download PDFInfo
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- CN202849399U CN202849399U CN 201220572175 CN201220572175U CN202849399U CN 202849399 U CN202849399 U CN 202849399U CN 201220572175 CN201220572175 CN 201220572175 CN 201220572175 U CN201220572175 U CN 201220572175U CN 202849399 U CN202849399 U CN 202849399U
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Abstract
The utility model discloses a device used for crushing an algae cell. The device used for crushing the algae cell comprises a high pressure reactor, an ozone generator and an air compressor, wherein the ozone generator and the air compressor are connected with the top part of the high pressure reactor through a pipeline; the high pressure reactor comprises a stirring device; and a vent valve and a pressure display device are further arranged on the high pressure reactor. By adopting the device used for crushing the algae cell disclosed by the utility model, ozone can be taken as medium to act on algae solution by gas-liquid reaction, and the ozone is evenly mixed with the algae solution under the action of the stirrer, so as to crush the algae cell. The device used for crushing the algae cell disclosed by the utility model has the characteristics of being simple in device, convenient to operate, good in wall breaking effect, easy to achieve automatic control and the like.
Description
Technical field
The utility model relates to the broken used device of a kind of alga cells, more specifically says to utilize ozone oxidation and pressurization-decompression acting in conjunction to carry out broken device in alga cells.
Background technology
Human productive life has produced the waste water of the nutritive substance such as a large amount of nitrogenous, phosphorus, these waste water enter the water bodys such as lake, river, bay via systems such as municipal drainage pipe networks, cause that algae breeds rapidly, dissolved oxygen content descends in the water body, water quality deterioration causes fish and other biological mortality then.Because the environmental degradation that pollution causes progressively increases the weight of, the degree that algae and water is polluted is also deepened year by year in recent years.When the eutrophication phenomenon appearred in water body, because the planktonic organism amount reproductions such as algae often make water body present blueness, redness, green etc., this phenomenon was called wawter bloom in rivers and lakes, be called red tide in the ocean.
It is the direct reflection of algae pollution heighten degree that red tide or wawter bloom frequently occur in the world.Red tide only occured 12 times in China in 1933 to 1979 46 years; Red tide has just occured 139 times in 5 years of nineteen ninety to 1994 year; Occured 79 in 2002, influence area is 10150 square kilometres; Occured 119 in 2003, influence area is 14550 square kilometres; Occured 82 in 2005, influence area is 27070 square kilometres; 2006 93, influence area is 19840 square kilometres; Larger area Noctiluca scientillans red tide appears in sea, Nan'ao, China Shenzhen in 2012.Along with being on the rise of Eutrophic Extent in each natural water, algal grown also becomes to the harm of water body and is on the rise, and wawter bloom, red tide phenomenon emerges in an endless stream so that fast and effeciently kill the research and development of the Measures of Algae in Water Body technology ever more important and urgent that seems in the water bodys such as ocean, lake.
In the world wide multiple algae removal technology is arranged at present, remove algae etc. such as physics except algae, chemical algae removing and biology.Along with the year by year minimizing of fossil energy in the world wide, study hotspot is from the single comprehensive utilization that the algae problem turns to algae that removes, and the bio-oil that broken alga cells wall extracts in the alginite is exactly an important direction.
All there is complex structure in the broken used device of alga cells both at home and abroad at present, operational difficulty, and restricted application, extraction step is various, the technical problems such as high cost.
Summary of the invention
The purpose of this utility model is in order to solve above-mentioned extraction element complex structure, operational difficulty, the technical problem such as extraction step is various, restricted application and provide a kind of alga cells broken used device.
The technical solution of the utility model
The broken used device of a kind of alga cells, comprise autoclave, ozonizer and air compressor machine, ozonizer is connected pipeline and is connected with the autoclave top with air compressor machine, described autoclave is with whipping appts, further described autoclave is provided with vent valve and pressure-display device, is tensimeter in the utility model one preferred embodiment.
Utilize the broken used device of above-mentioned a kind of alga cells that alga cells is carried out broken method, specifically comprise the following steps:
(1), with results algae liquid place autoclave, control 120～130 r/min rotate 5 min with algae liquid stirring and evenly mixing;
(3), open ozonizer and air compressor machine, the flow of control ozone and air gas mixture is 2 L/min, and control reactor rotating speed is that 120～130 r/min at the uniform velocity stir algae liquid, and the ozone, air and the algae liquid that pass into are mixed;
Wherein said ozone and air gas mixture are calculated by volume, i.e. ozone: air is 1:150;
(4), pressure condition is set to 0.4～0.8 MPa, keep 1～2 s is depressurized to normal pressure after the time, and then boosts to 0.4～0.8 MPa, keeps 1～2 s and be depressurized to normal pressure after the time again, the alga cells fragmentation is namely finished in 40～80 circulations repeatedly.
The beneficial effects of the utility model
The broken used device of a kind of alga cells of the present utility model owing to contain ozonizer, air compressor machine and autoclave, can realize utilizing the auxiliary By Ozone of pressure to the alga cells broken wall.Further the broken used device of a kind of alga cells of the present utility model is used for the alga cells broken wall, can effectively kill the algae in the water body, suppresses eutrophication to the harm of water body.Experimental results show that, be 0.8 MPa at peak pressure, pressurization-decompression cycle number is under 80 the condition, cell walls and cytolemma that can effective broken alga cells, make the cell content outflow, and easily oxidized materials such as chlorophyll can be by the further oxidation of ozone for some, thereby reduces algae required oxygen amount during corrupt oxidation in natural water.
Further, adopt the broken used device of a kind of alga cells of the present utility model that chlorella cells is carried out fragmentation, the chemical oxygen demand COD before and after the contrast experiment in the water body can find, dissolved organic matter content has significantly and increases in the system.
In addition, the broken used device of a kind of alga cells of the present utility model, equipment is simple, and is easy to operate, good to the crushing effect of alga cells, easily is automated control.
Description of drawings
The broken used device synoptic diagram of Fig. 1, alga cells of the present utility model, among the figure 1 be autoclave, 2 for ozonizer, 3 be that air compressor machine, 11 is the tensimeter of autoclave for the vent valve of autoclave, 13 for the whipping appts of autoclave, 12.
Embodiment
Also by reference to the accompanying drawings the broken used device of alga cells of the present utility model is described in further detail below by embodiment, but do not limit the utility model.
Used chlorella algae kind, used BG11 substratum are bought in Inst. of Hydrobiology, Chinese Academy of Sciences in the Application Example of the present utility model.
Algal species cultivation described in the Application Example of the present utility model and algae liquid obtain, being about to Inst. of Hydrobiology, Chinese Academy of Sciences, to buy the chlorella algae kind be 5% to be inoculated in the BG11 substratum by inoculum size, in 25 ℃ of Cerf PGX-350B intelligence incubators, 5000 lx, cultivate results algae liquid when treating chlorella growth to stationary phase under the Light To Dark Ratio 12h:12h condition.
Embodiment 1
The broken used device of a kind of alga cells, its synoptic diagram as shown in Figure 2, comprise autoclave 1, ozonizer 2 and air compressor machine 3, ozonizer 2 is connected with air compressor machine and is connected with autoclave 1 top by pipeline, described autoclave 1 is with whipping appts 11, and further described autoclave is provided with vent valve 12 and tensimeter 13.
Application Example 1
Utilize the broken used device of embodiment 1 described a kind of alga cells that alga cells is carried out broken method, specifically comprise the following steps:
(1), the algae liquid of measuring 200 ml results in the autoclave of 1 L, under 120～130 r/min conditions, stir 5 min;
(2), open ozonizer and air compressor machine, to be ozone by volume: air be that the ozone of 1:150 and the mixed gas control total flux of air composition are that 2 L/min are passed in the autoclave, continuation is at the uniform velocity stirred algae liquid with 120～130 r/min, and the ozone, air and the algae liquid that pass into are evenly mixed;
(3), control autoclave pressure is 0.6 MPa, when reaching 0.6 Mpa, kept for 1～2 s time, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure, and then boost to 0.6 MPa, when reaching 0.6 Mpa, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure again, and circulation is boosted, the step-down process namely obtains broken alga cells for 40 times.
Collect alga cells 100 ml of fragmentation of above-mentioned gained in the beaker of cleaning, solution temperature is 23 ℃ in the system, has no considerable change.Solvability COD content increases by 275 mg/L, and chlorophyll a has reduced by 0.3 mg/L, and content of chlorophyll b has reduced by 0.3 mg/L, and Chlorophyll content has reduced by 0.6 mg/L, and can extract the organism productive rate is the dried algae of 17.6g/100g.
Simultaneously can observe chlorella algae liquid is utilizing alga cells shredder assembly of the present utility model to carry out presenting green before the fragmentation, present white after the fragmentation, and the sediments microscope inspection result shows a large amount of alga cells fragments and generates, and has shown that thus this technique can successful broken chlorella cells and oxidation its cell content chlorophyll a and chlorophyll b.
The comparative example 1
The broken used device of alga cells the most commonly used is ultrasonic wave algae shredder assembly at present, and manufacturer is the letter Instr Ltd. in Shanghai, and model is JYD-1200L.
Utilize above-mentioned ultrasonic wave algae shredder assembly to the broken method of the carrying out of alga cells, specifically comprise the steps:
(1), measure cultured chlorella algae liquid 80 mL, place among the 200 mL beakers;
(2), ultrasonic head is inserted chlorella algae liquid central region, and ultrasonic frequency is set to 20 kHz, and power setting is 360 W;
(3), open the ultrasonic generator power supply, it is 30 min that ultrasonic time is set, and namely gets broken chlorella algae cell.
After above-mentioned alga cells fragmentation is complete, measure solution temperature, solvability COD, chlorophyll content, the result shows, after above-mentioned ultrasonic method processing, solution temperature rises to 70 ℃ in the system, and solvability COD content increases by 979 mg/L, chlorophyll a has increased by 3.5 mg/L, content of chlorophyll b has increased by 3.7 mg/L, and Chlorophyll content has increased by 7.2 mg/L, and can extract the organism productive rate is the dried algae of 14.1g/100g.
Compare by Application Example 1 and comparative example 1, can find out, after utilizing the broken used device of alga cells of the present utility model that alga cells is carried out fragmentation, the temperature of system is kept constant substantially, and system temperature rises very high after utilizing above-mentioned ultrasonic wave algae shredder assembly that alga cells is carried out fragmentation, this has illustrated that portion of energy is converted into heat loss in surrounding environment, is not effectively applied in the algae crushing process process.
Further, after the broken used device of alga cells of the present utility model carries out fragmentation to cell, the increase of solvability COD content only is 275 mg/L, reduced by 654 mg/L with respect to ultrasonication, show that thus the cracked rear organism of algae enters in the water body, partial organic substances has been reduced the water body resolvable chemical oxygen demand by ozone oxidation;
Further, after the broken used device of alga cells of the present utility model carries out fragmentation to cell, chlorophyll a has reduced by 0.3 mg/L, content of chlorophyll b has reduced by 0.3 mg/L, Chlorophyll content has reduced by 0.6 mg/L, and after utilizing above-mentioned ultrasonic wave algae shredder assembly that alga cells is carried out fragmentation, chlorophyll a has increased by 3.5 mg/L, content of chlorophyll b has increased by 3.7 mg/L, Chlorophyll content has increased by 7.2 mg/L, shown thus chlorophyll easily by ozone oxidation, very fast obtaining with ozone reaction removed after the cytoclasis, and the ultrasonic disruption device is then without this effect.
Further, after the broken used device of alga cells of the present utility model carries out fragmentation to cell, can extract the organism productive rate is the dried algae of 17.6g/100g, be the dried algae of 14.1g/100g and utilize above-mentioned ultrasonic wave algae shredder assembly that alga cells is carried out can extracting the organism productive rate after the fragmentation, some the easy extraction organism that has shown thus the generation of this technological process is suitable with ultrasonic method, has similar extraction efficiency.
In sum, use the broken used device of alga cells of the present utility model that alga cells is carried out fragmentation, not only effectively broken alga cells wall and cytolemma make the cell content outflow, obtain the dried algae productive rate of 17.6g/100g, suitable with the dried algae productive rate of ultrasonic technique 14.1g/100g.And easily oxidized materials such as chlorophyll can be by the further oxidation of ozone for some, system Determination of Chlorophyll content decrease 0.6 mg/L after broken, ultrasonic technique 7.2 mg/L that then risen, the broken used device of alga cells therefore of the present utility model can effectively reduce algae required oxygen amount during corrupt oxidation in natural water in the application of alga cells aspect broken.
Application Example 2
Utilize the broken used device of embodiment 1 described a kind of alga cells that alga cells is carried out broken method, specifically comprise the following steps:
(1), the chlorella solution of measuring 200 ml results in the autoclave of 1 L, under 120～130 r/min conditions, stir 5 min;
(2), open ozonizer and air compressor machine, to be ozone by volume: air be that the ozone of 1:150 and the mixed gas control total flux of air composition are that 2 L/min are passed in the autoclave, continuation is at the uniform velocity stirred algae liquid with 120～130 r/min, and the ozone, air and the algae liquid that pass into are evenly mixed;
(3), setting pressure is 0.6 MPa, when reaching 0.6 Mpa, kept for 1～2 s time, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure, and then boost to 0.6 MPa, when reaching 0.6 Mpa, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure again, and circulation is boosted, the step-down process namely obtains broken alga cells for 80 times.
Collect alga cells 100 ml of fragmentation of above-mentioned gained in the beaker of cleaning, solution temperature is 24 ℃ in the system, has no considerable change.Solvability COD content increases by 243 mg/L, and chlorophyll a has reduced by 0.1 mg/L, and content of chlorophyll b has reduced by 0.2 mg/L, and Chlorophyll content has reduced by 0.3 mg/L, and can extract the organism productive rate is the dried algae of 26.7g/100g.
Application Example 3
Utilize the broken used device of embodiment 1 described a kind of alga cells that alga cells is carried out broken method, specifically comprise the following steps:
(1), the chlorella solution of measuring 200 ml results in the autoclave of 1 L, under 120～130 r/min conditions, stir 5 min;
(2), open ozonizer and air compressor machine, to be ozone by volume: air be that the ozone of 1:150 and the mixed gas control total flux of air composition are that 2 L/min are passed in the autoclave, continuation is at the uniform velocity stirred algae liquid with 120～130 r/min, and the ozone, air and the algae liquid that pass into are evenly mixed;
(3), setting pressure is 0.8 MPa, when reaching 0.8 Mpa, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure, and then boost to 0.8 MPa, when reaching 0.8 Mpa, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure again, and circulation is boosted, the step-down process namely obtains broken alga cells for 80 times.
Collect alga cells 100 ml of fragmentation of above-mentioned gained in the beaker of cleaning, solution temperature is 24 ℃ in the system, has no considerable change.Solvability COD content increases by 1126 mg/L, and chlorophyll a has reduced by 0.3 mg/L, and content of chlorophyll b has reduced by 0.4 mg/L, and Chlorophyll content has reduced by 0.7 mg/L, and can extract the organism productive rate is the dried algae of 24.3g/100g.
Application Example 4
Utilize the broken used device of embodiment 1 described a kind of alga cells that alga cells is carried out broken method, specifically comprise the following steps:
(1), the chlorella solution of measuring 200 ml results in the autoclave of 1 L, under 120～130 r/min conditions, stir 5 min;
(2), open ozonizer and air compressor machine, to be ozone by volume: air be that the ozone of 1:150 and the mixed gas control total flux of air composition are that 2 L/min are passed in the autoclave, continuation is at the uniform velocity stirred algae liquid with 120～130 r/min, and the ozone, air and the algae liquid that pass into are evenly mixed;
(3), setting pressure is 0.4 MPa, when reaching 0.4 MPa, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure, and then boost to 0.4 MPa, when reaching 0.4 MPa, the vent valve of opening high pressure reactor makes Pressure Drop to normal pressure again, and circulation is boosted, the step-down process namely obtains broken alga cells for 80 times.
Collect alga cells 100 ml of fragmentation of above-mentioned gained in the beaker of cleaning, solution temperature is 24 ℃ in the system, has no considerable change.Solvability COD content increases by 201 mg/L, and chlorophyll a has reduced by 0.2 mg/L, and content of chlorophyll b has reduced by 0.2 mg/L, and Chlorophyll content has reduced by 0.4 mg/L, and can extract the organism productive rate is the dried algaes of 22.5 g/100g.
In sum, the broken used device of alga cells of the present utility model, owing to contain ozonizer, air compressor machine and autoclave, utilize it that alga cells is carried out fragmentation, can realize utilizing the auxiliary By Ozone of pressure to the alga cells broken wall, effectively to kill the algae in the water body, suppress eutrophication to the harm of water body.Experimental results show that, be 0.8 MPa at peak pressure, pressurization-decompression cycle number is under 80 the condition, cell walls and cytolemma that can effective broken alga cells, make the cell content outflow, and easily oxidized materials such as chlorophyll can be by the further oxidation of ozone for some, thereby reduces algae required oxygen amount during corrupt oxidation in natural water.
The above only is giving an example of embodiment of the present utility model; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the utility model know-why; can also make some improvement and modification, these improve and modification also should be considered as protection domain of the present utility model.
Claims (2)
1. the broken used device of alga cells is characterized in that comprising autoclave, ozonizer and air compressor machine, and described ozonizer is connected pipeline and is connected with the autoclave top with air compressor machine;
Described autoclave is with whipping appts, and described autoclave also is provided with vent valve and pressure-display device.
2. the broken used device of a kind of alga cells as claimed in claim 1 is characterized in that described pressure-display device is tensimeter.
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|---|---|---|---|
| CN 201220572175 CN202849399U (en) | 2012-11-02 | 2012-11-02 | Device used for crushing algae cell |
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| CN 201220572175 CN202849399U (en) | 2012-11-02 | 2012-11-02 | Device used for crushing algae cell |
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| CN202849399U true CN202849399U (en) | 2013-04-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352040A (en) * | 2018-03-13 | 2021-02-09 | 巴西石油公司 | Device and method for breaking microbial cells by extrusion |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352040A (en) * | 2018-03-13 | 2021-02-09 | 巴西石油公司 | Device and method for breaking microbial cells by extrusion |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130403 Termination date: 20131102 |