WO2008022569A1 - Method to hatch free swimming nauplii from artemia cysts - Google Patents

Method to hatch free swimming nauplii from artemia cysts Download PDF

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Publication number
WO2008022569A1
WO2008022569A1 PCT/CN2007/002501 CN2007002501W WO2008022569A1 WO 2008022569 A1 WO2008022569 A1 WO 2008022569A1 CN 2007002501 W CN2007002501 W CN 2007002501W WO 2008022569 A1 WO2008022569 A1 WO 2008022569A1
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artemia
cysts
hatching
artemia cysts
additives
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PCT/CN2007/002501
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French (fr)
Chinese (zh)
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Xinyu Fan
Yanli Ma
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Tianjin Jiayin Biology Feedstuffco., Ltd
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Publication of WO2008022569A1 publication Critical patent/WO2008022569A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Definitions

  • the present invention relates to a method for producing a live feed for aquaculture, and more particularly to a method for producing a free-flowing Artemia nauplii from a halogenated cyst. Background technique
  • Artemia nauplii are generally used as live feed for aquaculture, especially as a live feed for the early stages of marine fish and sea larvae. There is generally no live feed such as Artemia nauplii in the market, only the Artemia cysts, and the nauplii are hatched from the Artemia cysts. Hatching of Artemia cysts can be accomplished by incubation in incubation media. The Artemia cysts are fully dehydrated and can survive for a long time or even several years in a dry environment with low temperature, no oxygen and no light. The long storage capacity and short-term time, such as 24 hours, produce free-moving nauplii, making it the most convenient source of live feed for aquaculture.
  • Artemia cysts are collected from nature such as seaside salt fields and inland salt lakes. In these collected Artemia cysts, there are different proportions of dormant cysts and diapause cysts. The Artemia cysts are purified to remove debris, washed to remove salt, finally dried, and stored in a cold room to put them in a state of rest. The quality of Artemia cysts is determined by evaluating the parameters. The hatchability of Artemia cysts is the most concerned because it directly indicates that under artificial incubation conditions, it can be from a certain number of Artemia cysts. The amount of live feed (nausea) obtained.
  • the hatchability of Artemia cysts is usually expressed in terms of hatching rate (H%), which is the number of free-floating nauplii that hatch from 100 intact Artemia cysts.
  • H% hatching rate
  • the Artemia cysts in diapause or dormancy cannot continue to hatch into free-floating nauplii. Unless they are under conditions that promote hatching, the hatching of Artemia cysts needs to be done in the incubation medium.
  • Incubation The base is usually treated clean seawater, and its non-biological parameters include: salinity, pH, oxygen concentration, water temperature and light.
  • the parameters commonly used by various production units are: salinity 15 ⁇ 35g dissolved salt / liter; PH>8; oxygen concentration >5mg / L; water temperature 25 ⁇ 28 °C, the light intensity on the water surface should not be less than 20001ux.
  • Hatching of Artemia cysts is an extremely complex process that usually involves three phases: pre-development, umbrella, and actual incubation.
  • the hatching process according to the above given incubation conditions often fails to achieve the expected hatching effect. Therefore, special diapause inactivation methods are needed, such as prolonging the drying of the Artemia cysts.
  • Hydrogen peroxide solutions are currently commonly used to treat Artemia cysts to increase the hatching rate of Artemia cysts.
  • Another method of treating hydrogen peroxide is to directly add a solid compound capable of generating hydrogen peroxide such as perborate, percarbonate, magnesium peroxide or calcium peroxide to the incubation medium, but using this method.
  • a solid compound capable of generating hydrogen peroxide such as perborate, percarbonate, magnesium peroxide or calcium peroxide
  • the biggest disadvantage is that the pH of the incubation medium changes greatly, and the excessive pH may cause the death of the Artemia nauplii.
  • the object of the present invention is to overcome the above-mentioned shortcomings of the prior art and to provide a method for producing free-flowing Artemia nauplii from Artemia cysts with high hatching efficiency and convenience.
  • a method for producing free-flowing Artemia nauplii from Artemia cysts wherein the hatching of the Artemia cysts is carried out in an incubation medium, which is a treated clean seawater characterized by:
  • the cysts are added to the incubation medium simultaneously with the additive, which is an organic material containing one or more o-hydroxybenzenes.
  • the above method for producing free-flowing Artemia nauplii from Artemia cysts characterized in that: the additive is pyrogallic acid, gallic acid, gallic acid methyl ester, gallic acid ethyl ester, gallic acid propyl ester, Tannin, tannin, tea polyphenol, apple polyphenol, pine bark extract, grape seed extract, one or a combination of two or more.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that: the weight ratio of the Artemia cyst to the additive is 100: 0.5 to 4.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that: the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.2 to 0.5:100.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that: the additive is directly encapsulated on the Artemia cyst.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that the additive is packaged in a solid powder form with the Artemia cyst and packaged separately or separately.
  • the invention has the advantages that: by adding an organic substance containing one or more o-hydroxybenzenes as an additive in the incubation medium, the diapause mechanism of the Artemia cyst can be destroyed, and the diapause cyst is freely moved during hatching.
  • the Artemia nauplii can increase the hatching rate of Artemia cysts by about 90% within 24 hours and improve the hatching efficiency. Since the organic matter containing one or more o-hydroxybenzenes has a flocculant effect, the Artemia is not The larvae are easily separated from the incompletely hatched Artemia cysts.
  • the operation cost is low and the use is convenient; the parameter requirements for cyst density, salinity or water temperature during the hatching process are reduced, that is, the application range of the incubation medium is expanded, and the invention is particularly suitable for hatching the Artemia cysts produced in the inland salt lakes. deal with. detailed description
  • the Artemia cysts are prepared from eggs in the Russian inland lake, and are removed for impurities, salt, empty shells and dried; the incubation medium is 1 liter of treated clean seawater, and the non-biological parameters are: salt Degree 20g dissolved salt L, PH value 8.2, oxygen concentration 3mg/L, water temperature 28° (, the light intensity on the water surface is not less than 20001 UX; different types of additives are selected; the Artemia cysts and additives are simultaneously added to the incubation medium Incubate for 24 hours to check the hatching rate of Artemia cysts.
  • Example 1 The additive was pyrogallic acid (industrial pure). Adding 0.04 g of pyrogallic acid to 4 g of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 1, the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.404: 100 The hatching rate of Artemia cysts was 89%.
  • Example 2 The additive is pine bark extract (the original anthocyanin content is ⁇ 95%, produced by Ningbo Traditional Chinese Medicine Factory). Adding 0.05 g of pine bark extract to 2 g of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 2.5, and the ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.205: 100 The hatching rate of Artemia cysts was determined to be 92%.
  • Example 3 The additive was grape seed extract (original anthocyanin content ⁇ 95%, produced by Tianjin Jianfeng Natural Products Research and Development Co., Ltd.) and a mixture of gallic acid (industrial pure). Adding 0.08 grams of grape seed extract and 0.01 grams of gallic acid to 4 grams of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 2.25, the total amount of Artemia cysts and additives and the weight of the incubation medium. The ratio was 0.409: 100, and the hatching rate of Artemia cysts was 90%.
  • test results of the above examples show that: the Artemia cysts and the additives are simultaneously added to the incubation medium for incubation for 24 hours, and the hatching rate is about 90%, and the effect is remarkable; the method is simple and feasible, and the use effect is good.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
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  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Insects & Arthropods (AREA)
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  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

A method to hatch free swimming nauplii from Artemia cysts, including incubating the cysts in a hatching medium, in which the cysts are introduced simultaneously with a compound comprising one or more o-hydroxylphenyl structures, as an additive. The additives contain at least one substance selected from the group composed of pyrogallic acid, methyl gallate, tannic acid, vegetable tannin extract, tea polyphenol, apple polyphenol, pine bark extract and so on. A weight ratio of the cysts and additives is 100:0.5~4. The method achieves about 90% of the hatching percentage of the cysts in 24 hours by breaking diapause of said diapauzing cysts with said additives. The additives, which have a flocculent effect, enable free swimming nauplii to separate easily from incompletely incubating Artemia cysts. The coverage of hatching medium can be expanded, especially for hatching of Artemia cysts from an inland salt lake.

Description

一种从卤虫孢囊生产自由游动的卤虫无节幼体的方法 技术领域  Method for producing free-moving Artemia nauplii from Artemia cysts
本发明涉及一种用于水产养殖活体伺料的生产方法， 特别是一种从卤 虫孢囊生产自由游动的卤虫无节幼体的方法。 背景技术  The present invention relates to a method for producing a live feed for aquaculture, and more particularly to a method for producing a free-flowing Artemia nauplii from a halogenated cyst. Background technique
卤虫无节幼体一般用作水产养殖的活体饲料， 尤其是作为海鱼、 海虾 幼虫阶段早期的活体饲料。市场上一般没有卤虫无节幼体这样的活体饲料， 只有卤虫孢囊， 无节幼体就是从卤虫孢囊中孵化的。 卤虫孢囊的孵化可以 通过在孵化培养基中完成。 卤虫孢囊经充分脱水并在低温无氧无光照的干 燥环境下， 可以存活很长时间甚至长达几年。 超长的存储能力以及较短的 时间如 24小时内即可产生自由游动的无节幼体的特性，使其成为水产养殖 的最便利的活体饲料来源。  Artemia nauplii are generally used as live feed for aquaculture, especially as a live feed for the early stages of marine fish and sea larvae. There is generally no live feed such as Artemia nauplii in the market, only the Artemia cysts, and the nauplii are hatched from the Artemia cysts. Hatching of Artemia cysts can be accomplished by incubation in incubation media. The Artemia cysts are fully dehydrated and can survive for a long time or even several years in a dry environment with low temperature, no oxygen and no light. The long storage capacity and short-term time, such as 24 hours, produce free-moving nauplii, making it the most convenient source of live feed for aquaculture.
卤虫孢囊是从自然界如海边盐田和内陆盐湖中收集的。 在这些收集的 卤虫孢囊中， 存在不同比例的休眠孢囊和滞育孢囊。 将卤虫孢囊经纯化以 去除碎渣、 洗涤以去除盐分、 最后进行干燥处理和冷室保存， 使其处于休 眠状态。 卤虫孢囊的质量是通过评估参数来判定的， 其中卤虫孢囊的可孵 化能力最受到人们的关注， 因为它直接表明了在人工孵育条件下， 从一定 数量的卤虫孢囊中可以获得的活体饲料 (无节幼体)的数量。 卤虫孢囊的可 孵化能力通常用孵化率 (H%)来表示，即指从 100个完整卤虫孢囊中孵化出 的自由游动的无节幼体的个数。 处于滞育或休眠状态下的卤虫孢囊， 不能 继续孵化成自由游动的无节幼体， 除非处于促进孵化的条件下， 因此卤虫 孢囊的孵化需要在孵化培养基中完成， 孵化培养基通常为经过处理的清洁 海水， 其非生物参数包括： 盐度、 PH值、氧气浓度、 水温和光照。 目前各 生产单位通常采用的参数范围： 盐度为 15〜35g溶解盐 /升； PH>8; 氧气 浓度 >5mg / L; 水温 25〜28°C, 水面上的光照强度应不小于 20001ux。  Artemia cysts are collected from nature such as seaside salt fields and inland salt lakes. In these collected Artemia cysts, there are different proportions of dormant cysts and diapause cysts. The Artemia cysts are purified to remove debris, washed to remove salt, finally dried, and stored in a cold room to put them in a state of rest. The quality of Artemia cysts is determined by evaluating the parameters. The hatchability of Artemia cysts is the most concerned because it directly indicates that under artificial incubation conditions, it can be from a certain number of Artemia cysts. The amount of live feed (nausea) obtained. The hatchability of Artemia cysts is usually expressed in terms of hatching rate (H%), which is the number of free-floating nauplii that hatch from 100 intact Artemia cysts. The Artemia cysts in diapause or dormancy cannot continue to hatch into free-floating nauplii. Unless they are under conditions that promote hatching, the hatching of Artemia cysts needs to be done in the incubation medium. Incubation The base is usually treated clean seawater, and its non-biological parameters include: salinity, pH, oxygen concentration, water temperature and light. At present, the parameters commonly used by various production units are: salinity 15~35g dissolved salt / liter; PH>8; oxygen concentration >5mg / L; water temperature 25~28 °C, the light intensity on the water surface should not be less than 20001ux.
卤虫孢囊的孵化是一个极其复杂的过程， 通常包括三个阶段： 前发期 发育、 伞形期和实际孵化期。 但是影响卤虫孢囊孵化过程的因素很多， 按 照上述给定的孵化条件进行的孵化过程往往不能达到预期的孵化效果， 因 此需要采取特殊的滞育失活方法，例如延长干燥卤虫孢囊的冷冻存放时间、 重复吸水一脱水循环、 用某些化学物质作为添加剂来增加卤虫孢囊的孵化 量等。 目前通常使用过氧化氢溶液来处理卤虫孢囊以提高卤虫孢囊的孵化 率。采用过氧化氢溶液处理卤虫孢囊的缺点是： 1 )使用前针对不同卤虫株 系或同一株系不同批次的卤虫孢囊，需要进行大量的试验确定最适宜参数， 而且为了获得理想的孵化效果， 操作时还必须严格控制在该参数下进行， 这给使用者带来极大的不便； 2)实施过程中需要把水合后的卤虫孢囊转移 到过氧化氢溶液和相应的孵化培养基中， 而且这些步骤的进行对实施时间 的要求非常严格， 使操作工艺极其繁琐复杂； 3) 卤虫孢囊在经过过氧化氢 溶液后， 必须及时的用水洗涤以去除残存的过氧化氢， 甚至需要进行中和 处理， 势必增加处理成本。 另外一种采用过氧化氢的处理方法是在孵化培 养基中直接添加能产生过氧化氢的固体化合物如过硼酸盐、 过碳酸盐、 过 氧化镁或过氧化钙等， 但采用该方法的最大缺点是对孵化培养基的 PH值 改变较大， 过高的 PH值有可能造成卤虫无节幼体的死亡。 发明内容 Hatching of Artemia cysts is an extremely complex process that usually involves three phases: pre-development, umbrella, and actual incubation. However, there are many factors affecting the hatching process of Artemia cysts. The hatching process according to the above given incubation conditions often fails to achieve the expected hatching effect. Therefore, special diapause inactivation methods are needed, such as prolonging the drying of the Artemia cysts. Freeze storage time, repeated water absorption-dehydration cycle, use certain chemicals as additives to increase the hatching amount of Artemia cysts. Hydrogen peroxide solutions are currently commonly used to treat Artemia cysts to increase the hatching rate of Artemia cysts. The disadvantages of using hydrogen peroxide solution to treat Artemia cysts are: 1) A large number of experiments are required to determine the most suitable parameters for different Artemia strains or different batches of Artemia cysts before use. In order to obtain the ideal hatching effect, the operation must be strictly controlled under this parameter, which brings great inconvenience to the user; 2) The hydrated Artemia cysts need to be transferred to hydrogen peroxide during the implementation process. In the solution and the corresponding incubation medium, and the implementation time of these steps is very strict, making the operation process extremely complicated and complicated; 3) After the hydrogen peroxide solution, the Artemia cyst must be washed with water in time to remove Residual hydrogen peroxide, even requiring neutralization, is bound to increase processing costs. Another method of treating hydrogen peroxide is to directly add a solid compound capable of generating hydrogen peroxide such as perborate, percarbonate, magnesium peroxide or calcium peroxide to the incubation medium, but using this method. The biggest disadvantage is that the pH of the incubation medium changes greatly, and the excessive pH may cause the death of the Artemia nauplii. Summary of the invention
本发明的目的是克服上述现有技术中的缺点， 提供一种孵化效率高且 方便易行的从卤虫孢囊生产自由游动的卤虫无节幼体的方法。  SUMMARY OF THE INVENTION The object of the present invention is to overcome the above-mentioned shortcomings of the prior art and to provide a method for producing free-flowing Artemia nauplii from Artemia cysts with high hatching efficiency and convenience.
本发明的技术方案：  The technical solution of the invention:
一种从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 卤虫孢囊的孵 化在孵化培养基中进行， 孵化培养基为经过处理的清洁海水， 其特征在于： 将卤虫孢囊与添加剂同时加入到孵化培养基中， 添加剂为含有一个或多个 邻羟基苯的有机物。  A method for producing free-flowing Artemia nauplii from Artemia cysts, wherein the hatching of the Artemia cysts is carried out in an incubation medium, which is a treated clean seawater characterized by: The cysts are added to the incubation medium simultaneously with the additive, which is an organic material containing one or more o-hydroxybenzenes.
一种上述从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 其特征在 于： 添加剂为 焦性没食子酸、 没食子酸、 没食子酸甲脂、 没食子酸乙脂、 没食子酸丙脂、 单宁酸、 栲胶、 茶多酚、 苹果多酚、 松树皮提取物、 葡萄 籽提取物中的一种、 两种或两种以上的组合。  The above method for producing free-flowing Artemia nauplii from Artemia cysts, characterized in that: the additive is pyrogallic acid, gallic acid, gallic acid methyl ester, gallic acid ethyl ester, gallic acid propyl ester, Tannin, tannin, tea polyphenol, apple polyphenol, pine bark extract, grape seed extract, one or a combination of two or more.
一种上述从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 其特征在 于： 卤虫孢囊与添加剂的重量比为 100: 0.5〜4。  A method for producing a free-flowing Artemia nauplii from a brine shrimp cyst, characterized in that: the weight ratio of the Artemia cyst to the additive is 100: 0.5 to 4.
一种上述从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 其特征在 于： 卤虫孢囊和添加剂的总量与孵化培养基的重量比为 0.2〜0.5： 100。  A method for producing a free-flowing Artemia nauplii from a brine shrimp cyst, characterized in that: the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.2 to 0.5:100.
一种上述从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 其特征在 于： 添加剂直接包裹在卤虫孢囊上。  A method for producing a free-flowing Artemia nauplii from a brine shrimp cyst, characterized in that: the additive is directly encapsulated on the Artemia cyst.
一种上述从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 其特征在 于：添加剂以固体粉末的形式与卤虫孢囊混合后一起包装或分开单独包装。  A method for producing a free-flowing Artemia nauplii from a brine shrimp cyst, characterized in that the additive is packaged in a solid powder form with the Artemia cyst and packaged separately or separately.
本发明的优点是： 由于在孵化培养基中加入了含有一个或多个邻羟基 苯的有机物作为添加剂， 能够破坏卤虫孢囊的滞育机制， 促使滞育孢囊在 孵化期间产生自由游动的卤虫无节幼体，可使卤虫孢囊 24小时内的孵化率 达到 90%左右， 并提高了孵化效率； 由于含有一个或多个邻羟基苯的有机 物具有絮凝剂作用， 使卤虫无节幼体与未完全孵化的卤虫孢囊容易分离， 操作成本低， 使用方便； 降低了孵化过程中对孢囊密度、 盐度或水温的参 数要求， 即扩大了孵化培养基的适用范围， 特别适用于对内陆盐湖出产的 卤虫孢囊进行孵化处理。 具体实施方式 The invention has the advantages that: by adding an organic substance containing one or more o-hydroxybenzenes as an additive in the incubation medium, the diapause mechanism of the Artemia cyst can be destroyed, and the diapause cyst is freely moved during hatching. The Artemia nauplii can increase the hatching rate of Artemia cysts by about 90% within 24 hours and improve the hatching efficiency. Since the organic matter containing one or more o-hydroxybenzenes has a flocculant effect, the Artemia is not The larvae are easily separated from the incompletely hatched Artemia cysts. The operation cost is low and the use is convenient; the parameter requirements for cyst density, salinity or water temperature during the hatching process are reduced, that is, the application range of the incubation medium is expanded, and the invention is particularly suitable for hatching the Artemia cysts produced in the inland salt lakes. deal with. detailed description
本实施例中卤虫孢囊采用产于俄罗斯内陆湖的虫卵，并进行去除杂质、 盐分、空壳和干燥处理； 孵化培养基均为 1升经过处理的清洁海水， 其非生 物参数为： 盐度 20g溶解盐L、 PH值 8.2、 氧气浓度 3mg/L、 水温 28° ( 、 水面上的光照强度不小于 20001UX; 选用不同类型的添加剂； 将卤虫孢囊和 添加剂同时加入孵化培养基中孵化 24小时， 检测卤虫孢囊的孵化率。 In this embodiment, the Artemia cysts are prepared from eggs in the Russian inland lake, and are removed for impurities, salt, empty shells and dried; the incubation medium is 1 liter of treated clean seawater, and the non-biological parameters are: salt Degree 20g dissolved salt L, PH value 8.2, oxygen concentration 3mg/L, water temperature 28° (, the light intensity on the water surface is not less than 20001 UX; different types of additives are selected; the Artemia cysts and additives are simultaneously added to the incubation medium Incubate for 24 hours to check the hatching rate of Artemia cysts.
实施例 1 : 添加剂为 焦性没食子酸 （工业纯） 。 在 4克卤虫孢囊中 添加 0.04克焦性没食子酸， 卤虫抱囊与添加剂的重量比为 100: 1， 卤虫孢 囊和添加剂的总量与孵化培养基的重量比为 0.404: 100， 测得卤虫孢囊的 孵化率为 89 %。  Example 1: The additive was pyrogallic acid (industrial pure). Adding 0.04 g of pyrogallic acid to 4 g of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 1, the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.404: 100 The hatching rate of Artemia cysts was 89%.
实施例 2: 添加剂为 松树皮提取物 （原花青素含量≥95%、 宁波市中 药制药厂生产） 。 在 2克卤虫孢囊中添加 0.05克松树皮提取物， 卤虫孢囊 与添加剂的重量比为 100: 2.5, 卤虫孢囊和添加剂的总量与孵化培养基的 重量比为 0.205: 100, 测得卤虫孢囊的孵化率为 92 %。  Example 2: The additive is pine bark extract (the original anthocyanin content is ≥95%, produced by Ningbo Traditional Chinese Medicine Factory). Adding 0.05 g of pine bark extract to 2 g of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 2.5, and the ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.205: 100 The hatching rate of Artemia cysts was determined to be 92%.
实施例 3 : 添加剂为葡萄籽提取物（原花青素含量≥95%、天津尖峰天 然产物研究开发有限公司生产） 和没食子酸的混合物 （工业纯） 。 在 4克 卤虫孢囊中添加 0.08克葡萄籽提取物和 0.01克没食子酸，卤虫孢囊与添加 剂的重量比为 100: 2.25 , 卤虫孢囊和添加剂的总量与孵化培养基的重量比 为 0.409: 100, 测得卤虫孢囊的孵化率为 90%。  Example 3: The additive was grape seed extract (original anthocyanin content ≥ 95%, produced by Tianjin Jianfeng Natural Products Research and Development Co., Ltd.) and a mixture of gallic acid (industrial pure). Adding 0.08 grams of grape seed extract and 0.01 grams of gallic acid to 4 grams of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 2.25, the total amount of Artemia cysts and additives and the weight of the incubation medium. The ratio was 0.409: 100, and the hatching rate of Artemia cysts was 90%.
上述实施例检测结果可以看出： 将卤虫孢囊与添加剂同时加入到孵化 培养基中进行 24小时孵化， 孵化率均达到 90 %左右， 效果显著； 本方法 简易可行， 使用效果良好。  The test results of the above examples show that: the Artemia cysts and the additives are simultaneously added to the incubation medium for incubation for 24 hours, and the hatching rate is about 90%, and the effect is remarkable; the method is simple and feasible, and the use effect is good.

Claims

权 利 要 求 书 Claim
1.一种从卤虫孢囊生产自由游动的卤虫无节幼体的方法， 卤虫孢囊的 孵化在孵化培养基中进行， 孵化培养基为经过处理的清洁海水， 其特征在 于： 将卤虫孢囊与添加剂同时加入到孵化培养基中， 添加剂为含有一个或 多个邻羟基苯的有机物。 A method for producing free-flowing Artemia nauplii from Artemia cysts, wherein hatching of the Artemia cysts is carried out in an incubation medium, the hatching medium being treated clean seawater, characterized in that The Artemia cyst and the additive are simultaneously added to the incubation medium, and the additive is an organic substance containing one or more o-hydroxybenzenes.
2.根据权利要求 1所述的方法， 其特征在于： 添加剂为 焦性没食子酸、 没食子酸、 没食子酸甲脂、 没食子酸乙脂、 没食子酸丙脂、 单宁酸、 栲胶、 茶多酚、 苹果多酚、 松树皮提取物、 葡萄籽提取物中的一种、 两种或两种 以上的组合。  The method according to claim 1, wherein the additive is pyrogallic acid, gallic acid, methyl gallate, ethyl gallate, gallic acid gall, tannic acid, tannin, tea polyphenol , apple polyphenols, pine bark extract, one of grape seed extracts, a combination of two or more.
3.根,据权利要求 1 所述的方法， 其特征在于： 卤虫孢囊与添加剂的重 量比为 100： 0.5〜4。  3. Root according to claim 1, characterized in that the weight ratio of Artemia cysts to additives is 100: 0.5 to 4.
4.根据权利要求 1 所述的方法， 其特征在于： 卤虫孢囊和添加剂的总 量与孵化培养基的重量比为 0.2〜0.5： 100。  The method according to claim 1, wherein the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.2 to 0.5:100.
5.根据权利要求 1 所述的方法， 其特征在于： 添加剂直接包裹在卤虫 孢囊上。  5. The method of claim 1 wherein: the additive is encapsulated directly on the Artemia cyst.
6.根据权利要求 1所述的方法， 其特征在于： 添加剂以固体粉末的形式 与卤虫孢囊混合后一起包装或分开单独包装。  The method according to claim 1, wherein the additive is mixed with the Artemia cyst after being mixed as a solid powder, or separately packaged separately.
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