WO2008022569A1 - Procédé pour faire éclore des nauplii nageant librement à partir de cystes d'artémies - Google Patents

Procédé pour faire éclore des nauplii nageant librement à partir de cystes d'artémies Download PDF

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Publication number
WO2008022569A1
WO2008022569A1 PCT/CN2007/002501 CN2007002501W WO2008022569A1 WO 2008022569 A1 WO2008022569 A1 WO 2008022569A1 CN 2007002501 W CN2007002501 W CN 2007002501W WO 2008022569 A1 WO2008022569 A1 WO 2008022569A1
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WO
WIPO (PCT)
Prior art keywords
artemia
cysts
hatching
artemia cysts
additives
Prior art date
Application number
PCT/CN2007/002501
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English (en)
Chinese (zh)
Inventor
Xinyu Fan
Yanli Ma
Original Assignee
Tianjin Jiayin Biology Feedstuffco., Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Jiayin Biology Feedstuffco., Ltd filed Critical Tianjin Jiayin Biology Feedstuffco., Ltd
Publication of WO2008022569A1 publication Critical patent/WO2008022569A1/fr

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Definitions

  • the present invention relates to a method for producing a live feed for aquaculture, and more particularly to a method for producing a free-flowing Artemia nauplii from a halogenated cyst. Background technique
  • Artemia nauplii are generally used as live feed for aquaculture, especially as a live feed for the early stages of marine fish and sea larvae. There is generally no live feed such as Artemia nauplii in the market, only the Artemia cysts, and the nauplii are hatched from the Artemia cysts. Hatching of Artemia cysts can be accomplished by incubation in incubation media. The Artemia cysts are fully dehydrated and can survive for a long time or even several years in a dry environment with low temperature, no oxygen and no light. The long storage capacity and short-term time, such as 24 hours, produce free-moving nauplii, making it the most convenient source of live feed for aquaculture.
  • Artemia cysts are collected from nature such as seaside salt fields and inland salt lakes. In these collected Artemia cysts, there are different proportions of dormant cysts and diapause cysts. The Artemia cysts are purified to remove debris, washed to remove salt, finally dried, and stored in a cold room to put them in a state of rest. The quality of Artemia cysts is determined by evaluating the parameters. The hatchability of Artemia cysts is the most concerned because it directly indicates that under artificial incubation conditions, it can be from a certain number of Artemia cysts. The amount of live feed (nausea) obtained.
  • the hatchability of Artemia cysts is usually expressed in terms of hatching rate (H%), which is the number of free-floating nauplii that hatch from 100 intact Artemia cysts.
  • H% hatching rate
  • the Artemia cysts in diapause or dormancy cannot continue to hatch into free-floating nauplii. Unless they are under conditions that promote hatching, the hatching of Artemia cysts needs to be done in the incubation medium.
  • Incubation The base is usually treated clean seawater, and its non-biological parameters include: salinity, pH, oxygen concentration, water temperature and light.
  • the parameters commonly used by various production units are: salinity 15 ⁇ 35g dissolved salt / liter; PH>8; oxygen concentration >5mg / L; water temperature 25 ⁇ 28 °C, the light intensity on the water surface should not be less than 20001ux.
  • Hatching of Artemia cysts is an extremely complex process that usually involves three phases: pre-development, umbrella, and actual incubation.
  • the hatching process according to the above given incubation conditions often fails to achieve the expected hatching effect. Therefore, special diapause inactivation methods are needed, such as prolonging the drying of the Artemia cysts.
  • Hydrogen peroxide solutions are currently commonly used to treat Artemia cysts to increase the hatching rate of Artemia cysts.
  • Another method of treating hydrogen peroxide is to directly add a solid compound capable of generating hydrogen peroxide such as perborate, percarbonate, magnesium peroxide or calcium peroxide to the incubation medium, but using this method.
  • a solid compound capable of generating hydrogen peroxide such as perborate, percarbonate, magnesium peroxide or calcium peroxide
  • the biggest disadvantage is that the pH of the incubation medium changes greatly, and the excessive pH may cause the death of the Artemia nauplii.
  • the object of the present invention is to overcome the above-mentioned shortcomings of the prior art and to provide a method for producing free-flowing Artemia nauplii from Artemia cysts with high hatching efficiency and convenience.
  • a method for producing free-flowing Artemia nauplii from Artemia cysts wherein the hatching of the Artemia cysts is carried out in an incubation medium, which is a treated clean seawater characterized by:
  • the cysts are added to the incubation medium simultaneously with the additive, which is an organic material containing one or more o-hydroxybenzenes.
  • the above method for producing free-flowing Artemia nauplii from Artemia cysts characterized in that: the additive is pyrogallic acid, gallic acid, gallic acid methyl ester, gallic acid ethyl ester, gallic acid propyl ester, Tannin, tannin, tea polyphenol, apple polyphenol, pine bark extract, grape seed extract, one or a combination of two or more.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that: the weight ratio of the Artemia cyst to the additive is 100: 0.5 to 4.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that: the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.2 to 0.5:100.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that: the additive is directly encapsulated on the Artemia cyst.
  • a method for producing a free-flowing Artemia nauplii from a brine shrimp cyst characterized in that the additive is packaged in a solid powder form with the Artemia cyst and packaged separately or separately.
  • the invention has the advantages that: by adding an organic substance containing one or more o-hydroxybenzenes as an additive in the incubation medium, the diapause mechanism of the Artemia cyst can be destroyed, and the diapause cyst is freely moved during hatching.
  • the Artemia nauplii can increase the hatching rate of Artemia cysts by about 90% within 24 hours and improve the hatching efficiency. Since the organic matter containing one or more o-hydroxybenzenes has a flocculant effect, the Artemia is not The larvae are easily separated from the incompletely hatched Artemia cysts.
  • the operation cost is low and the use is convenient; the parameter requirements for cyst density, salinity or water temperature during the hatching process are reduced, that is, the application range of the incubation medium is expanded, and the invention is particularly suitable for hatching the Artemia cysts produced in the inland salt lakes. deal with. detailed description
  • the Artemia cysts are prepared from eggs in the Russian inland lake, and are removed for impurities, salt, empty shells and dried; the incubation medium is 1 liter of treated clean seawater, and the non-biological parameters are: salt Degree 20g dissolved salt L, PH value 8.2, oxygen concentration 3mg/L, water temperature 28° (, the light intensity on the water surface is not less than 20001 UX; different types of additives are selected; the Artemia cysts and additives are simultaneously added to the incubation medium Incubate for 24 hours to check the hatching rate of Artemia cysts.
  • Example 1 The additive was pyrogallic acid (industrial pure). Adding 0.04 g of pyrogallic acid to 4 g of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 1, the weight ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.404: 100 The hatching rate of Artemia cysts was 89%.
  • Example 2 The additive is pine bark extract (the original anthocyanin content is ⁇ 95%, produced by Ningbo Traditional Chinese Medicine Factory). Adding 0.05 g of pine bark extract to 2 g of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 2.5, and the ratio of the total amount of Artemia cysts and additives to the incubation medium is 0.205: 100 The hatching rate of Artemia cysts was determined to be 92%.
  • Example 3 The additive was grape seed extract (original anthocyanin content ⁇ 95%, produced by Tianjin Jianfeng Natural Products Research and Development Co., Ltd.) and a mixture of gallic acid (industrial pure). Adding 0.08 grams of grape seed extract and 0.01 grams of gallic acid to 4 grams of Artemia cysts, the weight ratio of Artemia cysts to additives is 100: 2.25, the total amount of Artemia cysts and additives and the weight of the incubation medium. The ratio was 0.409: 100, and the hatching rate of Artemia cysts was 90%.
  • test results of the above examples show that: the Artemia cysts and the additives are simultaneously added to the incubation medium for incubation for 24 hours, and the hatching rate is about 90%, and the effect is remarkable; the method is simple and feasible, and the use effect is good.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Birds (AREA)
  • Insects & Arthropods (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé pour faire éclore des nauplii nageant librement à partir de cystes d'artémies. Ce procédé consiste à : faire incuber les cystes dans un milieu d'incubation dans lequel les cystes sont introduits en même temps qu'un composé comprenant ou une plusieurs structures o-hydroxylphényle en tant qu'additif. Les additifs comprennent au moins une substance qui est sélectionnée dans le groupe comprenant l'acide pyrogallique, le gallate de méthyle, l'acide tannique, l'extrait de tanin végétal, le polyphénol de thé, le polyphénol de pomme, l'extrait d'écorce de pin, etc. Le rapport pondéral entre les cystes et les additifs est de 100:0,5~4. Le procédé selon l'invention permet de faire éclore approximativement 90 % des cystes en 24 heures par rupture de la diapause des cystes en phase de diapause à l'aide desdits additifs. Ces additifs, qui ont un effet floculant, permettent aux nauplii nageant librement de se séparer facilement des cystes d'artémies dont l'incubation n'est pas entièrement terminée. Le champ d'application dudit milieu d'incubation peut être élargi, en particulier pour faire éclore des cystes d'artémies provenant d'un lac salé intérieur.
PCT/CN2007/002501 2006-08-18 2007-08-20 Procédé pour faire éclore des nauplii nageant librement à partir de cystes d'artémies WO2008022569A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN200610015369.5 2006-08-18
CN200610015369 2006-08-18
CNA2007101374098A CN101081015A (zh) 2006-08-18 2007-07-11 一种从卤虫孢囊生产自由游动的卤虫无节幼体的方法
CN200710137409.8 2007-07-11

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WO2008022569A1 true WO2008022569A1 (fr) 2008-02-28

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CN (1) CN101081015A (fr)
WO (1) WO2008022569A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103563860A (zh) * 2013-11-22 2014-02-12 天津海友佳音生物科技股份有限公司 一种提高弱光环境下滞育卤虫卵孵化率的方法
CN106719168A (zh) * 2016-12-14 2017-05-31 中国电建集团贵阳勘测设计研究院有限公司 一种鱼类增殖放流站用集约型卤虫孵化装置及其使用方法
CN111789068A (zh) * 2020-07-16 2020-10-20 滨州市胜英水产有限公司 一种丰年虫卵孵化设备及其孵化方法
CN112167131A (zh) * 2020-09-30 2021-01-05 天津科技大学 红色嗜盐古菌盐红菌菌株及其强化卤虫在水产育苗或养殖中的应用
CN113615610A (zh) * 2021-07-29 2021-11-09 天津海友佳音生物科技股份有限公司 一种提高卤虫卵孵化率的孵化方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102027891B (zh) * 2009-09-29 2013-03-13 天津市佳音生物饲料有限公司 提高卤虫卵孵化率的方法
CN102106322B (zh) * 2011-01-28 2012-05-23 深圳市龙科源水产养殖有限公司 卤虫封闭式培养系统及培养方法
CN103651261B (zh) * 2012-09-21 2016-04-27 上海海洋大学 利用生物絮凝体悬浮液孵化卤虫的方法
CN104273098B (zh) * 2014-10-21 2016-08-24 天津海友佳音生物科技股份有限公司 一种紫外光下孵化滞育卤虫卵的方法
CN107455615B (zh) * 2017-07-21 2020-05-22 浙江海洋大学 一种适于真蛸浮游幼体培养的卤虫营养强化方法

Citations (4)

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WO1996012407A1 (fr) * 1994-10-21 1996-05-02 Inve Aquaculture N.V. Procede de reduction de la contamination bacterienne en aquaculture
EP1195088A1 (fr) * 2000-10-05 2002-04-10 Inve Technologies N.V. Procédé pour produire des Artemia nauplii nageant librement et kystes emballés pour usage dans ce procédé
WO2004091307A2 (fr) * 2003-04-08 2004-10-28 Advanced Bionutriton Corporation Additifs alimentaires destines a proteger des animaux terrestres et aquatiques contre une infection pathologique
EP1767101A1 (fr) * 2005-09-26 2007-03-28 Inve Technologies N.V. Procédé pour augmenter le pourcentage d'éclosion de kystes d'artémies inactives

Patent Citations (4)

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WO1996012407A1 (fr) * 1994-10-21 1996-05-02 Inve Aquaculture N.V. Procede de reduction de la contamination bacterienne en aquaculture
EP1195088A1 (fr) * 2000-10-05 2002-04-10 Inve Technologies N.V. Procédé pour produire des Artemia nauplii nageant librement et kystes emballés pour usage dans ce procédé
WO2004091307A2 (fr) * 2003-04-08 2004-10-28 Advanced Bionutriton Corporation Additifs alimentaires destines a proteger des animaux terrestres et aquatiques contre une infection pathologique
EP1767101A1 (fr) * 2005-09-26 2007-03-28 Inve Technologies N.V. Procédé pour augmenter le pourcentage d'éclosion de kystes d'artémies inactives

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103563860A (zh) * 2013-11-22 2014-02-12 天津海友佳音生物科技股份有限公司 一种提高弱光环境下滞育卤虫卵孵化率的方法
CN106719168A (zh) * 2016-12-14 2017-05-31 中国电建集团贵阳勘测设计研究院有限公司 一种鱼类增殖放流站用集约型卤虫孵化装置及其使用方法
CN111789068A (zh) * 2020-07-16 2020-10-20 滨州市胜英水产有限公司 一种丰年虫卵孵化设备及其孵化方法
CN112167131A (zh) * 2020-09-30 2021-01-05 天津科技大学 红色嗜盐古菌盐红菌菌株及其强化卤虫在水产育苗或养殖中的应用
CN112167131B (zh) * 2020-09-30 2023-03-10 天津科技大学 红色嗜盐古菌盐红菌菌株及其强化卤虫在水产育苗或养殖中的应用
CN113615610A (zh) * 2021-07-29 2021-11-09 天津海友佳音生物科技股份有限公司 一种提高卤虫卵孵化率的孵化方法

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