CN103947818B - A kind of preparation method of squid active peptide - Google Patents
A kind of preparation method of squid active peptide Download PDFInfo
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- CN103947818B CN103947818B CN201410162537.8A CN201410162537A CN103947818B CN 103947818 B CN103947818 B CN 103947818B CN 201410162537 A CN201410162537 A CN 201410162537A CN 103947818 B CN103947818 B CN 103947818B
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Abstract
The invention discloses a kind of method extracting squid active peptide from squid, comprise the steps: that Feedstock treating, ultrasonic wave process, HIGH PRESSURE TREATMENT obtain finished product.Compared to existing technology, the time shortens dramatically.
Description
Technical field
The present invention relates to a kind of preparation method of active peptide, be specifically related to a kind of method extracting squid active peptide from squid.
Background technology
Squid, also claims squid, squid, and belonging to mollusc, is the one of cuttlefish.Squid is a kind of famous and precious marine product, is important ocean siphonopods aquatic products thing, is distributed widely in each sea area in the Atlantic Ocean, the Indian Ocean and the Pacific Ocean.China squid year quantity of the catch, at about 500,000 tons, is domestic main ocean fishery kind.Also be one of kind with potentiality to be exploited most in current deep-sea fishing.Squid is rich in calcium, phosphorus, ferro element, is beneficial to skeleton development and hematopoiesis, effectively can treat anaemia; Squid also containing a large amount of taurines, can suppress the cholesterol level in blood, fatigue-relieving, recovers eyesight, improves liver function; Polypeptide contained by it and selenium have antiviral, anti-actinism.There is head, foot, liver and the epidermis the like waste accounting for squid body weight 30% to produce in squid process, and biologically active peptide can be extracted from the liver of squid, there is the effect of hypotensive, antiviral, anti-ray.
At present, from squid, obtain the method for active peptide, have acid-alkali treatment method, enzyme decomposition method, microbe fermentation method etc.As Chinese patent CN102217699B, disclose a kind of preparation method of squid skin protein active peptide, squid skin is cleaned and is cut into bulk, add NaOH aqueous solution soaking 2-8d and decolour, then through being washed to pH neutrality, add Ca (OH)
2solution soaks 2-4d and carries out fixation, again through being washed to pH neutrality, in the pH neutral water of 55-75 DEG C, process 0.5-1.5h filter, removing insoluble protein, extraction obtains fish skin protein liquor, finally, by this liquid in 110-121 DEG C of HIGH PRESSURE TREATMENT 30-120min, both obtained squid skin protein active peptide, the time of whole operation is oversize.And for example Chinese patent CN102217699B, disclose the preparation method of a kind of marine source BacillusbarbaricusSCSIO02429CCTCCNO:M20102130 and the little tripe of a kind of squid, BacillusbarbaricusSCSIO02429 is added have in induction product enzyme fermentation culture medium and ferment, obtain hydrolysis and use thick enzyme solutions, then add thick enzyme solutions after squid viscera being broken into pulpous state and carry out enzymolysis, obtain the crude protein zymolyte that sugar side chains is destroyed, add bromelain again and carry out enzymolysis, the zymolyte obtained is through leaving standstill, crude fat layer is separated with water layer, dry after removing fat deposit, obtain squid small peptide, the duration of whole operation is longer.The equal Shortcomings of existing method, this constrains intensive processing and the application of squid to a certain extent.
Summary of the invention
The invention provides a kind of technical maturity, be applicable to the method extracting squid active peptide from squid of production.
The present invention is achieved through the following technical solutions:
From squid, extract a method for squid active peptide, comprise the steps:
A. Feedstock treating: add pure water after new Fresh squid being cleaned and rub;
B. decolouring, ultrasonic wave process: add dilute alkaline soln, ultrasonic wave process 20-40min, operative temperature 50-65 DEG C in the squid of rubbing, power is 200-350W, it is neutral for washing to pH with pure water, filters, obtains squid protein extract;
C. HIGH PRESSURE TREATMENT: the squid protein extract obtained by step b, puts into pressure pan, is forced into 3kgf/cm
2, and keep 10-20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter, obtain squid active peptide.
Preferably, in step a, the weight ratio of squid and pure water is 1:1-4;
Preferably, in step b, dilute alkaline soln is KOH solution or the NaOH solution of 0.01-0.50mol/L, and the amount added is the 20-40% of new Fresh squid quality.
Preferably, ultrasonic treatment time 30min in step b.
Preferably, ul-trasonic irradiation temperature 60 C in step b.
Preferably, ultrasonic power 300W in step b.
Adopt method of the present invention with new Fresh squid for raw material extracts squid polypeptide, compared with prior art, advantage of the present invention:
1, the advanced technology such as ultrasonic wave, high pressure comprehensively makes for preparation squid polypeptide by the bright method of this law, and process innovation, is applicable to large production.Technical maturity, reliable, this method can cost-saving, reduce energy consumption, improve extraction efficiency.
2, desolventing technology of the present invention carries out in hyperacoustic condition, and compare the decolouring of direct alkali lye, time shorten is about 1/10th, and, while decolouring, also in the extraction to squid albumen, make to extract and decolour to carry out simultaneously.
3, the present invention is by HIGH PRESSURE TREATMENT, and make squid chopping up proteins, obtain squid active peptide, the time is short, and efficiency is high.
4, use high pressure technique to obtain polypeptide, enzyme process decomposition compared to existing technology, microbial decomposition, the time also shortens dramatically, and accessory substance is few.
Detailed description of the invention
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
From squid, extract a method for squid active peptide, comprise the steps:
A. Feedstock treating: add pure water 10kg after new for 10kg Fresh squid being cleaned and rub;
B. decolouring, ultrasonic wave process: the KOH solution 4kg adding 0.01mol/L in the squid of rubbing, ultrasonic wave process 20min, operative temperature 65 DEG C, power is 350W, and it is neutral for washing to pH with pure water, filters, obtains squid protein extract;
C. HIGH PRESSURE TREATMENT: the squid protein extract obtained by step b, puts into pressure pan, is forced into 3kgf/cm
2, and keep 10min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter, obtain squid active peptide 1.52kg, content 91.5%.
Embodiment 2:
From squid, extract a method for squid active peptide, comprise the steps:
A. Feedstock treating: add pure water 40kg after new for 10kg Fresh squid being cleaned and rub;
B. decolouring, ultrasonic wave process: the NaOH solution 2kg adding 0.50mol/L in the squid of rubbing, ultrasonic wave process 40min, operative temperature 50 DEG C, power is 300W, and it is neutral for washing to pH with pure water, filters, obtains squid protein extract;
C. HIGH PRESSURE TREATMENT: the squid protein extract obtained by step b, puts into pressure pan, is forced into 3kgf/cm
2, and keep 20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter, obtain squid active peptide 1.48kg, content 92.1%.
Embodiment 3:
From squid, extract a method for squid active peptide, comprise the steps:
A. Feedstock treating: add pure water 20kg after new for 10kg Fresh squid being cleaned and rub;
B. decolouring, ultrasonic wave process: the NaOH solution 3kg adding 0.25mol/L in the squid of rubbing, ultrasonic wave process 30min, operative temperature 65 DEG C, power is 300W, and it is neutral for washing to pH with pure water, filters, obtains squid protein extract;
C. HIGH PRESSURE TREATMENT: the squid protein extract obtained by step b, puts into pressure pan, is forced into 3kgf/cm
2, and keep 15min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter, obtain squid active peptide 1.55kg, content 93.4%.
Embodiment 4:
From squid, extract a method for squid active peptide, comprise the steps:
A. Feedstock treating: add pure water 30kg after new for 10kg Fresh squid being cleaned and rub;
B. decolouring, ultrasonic wave process: the KOH solution 3kg adding 0.30mol/L in the squid of rubbing, ultrasonic wave process 25min, operative temperature 60 DEG C, power is 250W, and it is neutral for washing to pH with pure water, filters, obtains squid protein extract;
C. HIGH PRESSURE TREATMENT: the squid protein extract obtained by step b, puts into pressure pan, is forced into 3kgf/cm
2, and keep 20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter, obtain squid active peptide 1.58kg, content 93.5%.
Claims (6)
1. from squid, extract a method for squid active peptide, comprise the steps:
A. Feedstock treating: add pure water after new Fresh squid being cleaned and rub;
B. decolouring, ultrasonic wave process: add dilute alkaline soln, ultrasonic wave process 20-40min, operative temperature 50-65 DEG C in the squid of rubbing, power is 200-350W, it is neutral for washing to pH with pure water, filters, obtains squid protein extract;
C. HIGH PRESSURE TREATMENT: the squid protein extract obtained by step b, puts into pressure pan, is forced into 3kgf/cm
2, and keep 10-20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter, obtain squid active peptide.
2. the method extracting squid active peptide from squid according to claim 1, is characterized in that: in step a, the weight ratio of squid and pure water is 1:1-4.
3. the method extracting squid active peptide from squid according to claim 1, is characterized in that: in step b, dilute alkaline soln is KOH solution or the NaOH solution of 0.01-0.50mol/L, and the amount added is the 20-40% of new Fresh squid quality.
4. the method extracting squid active peptide from squid according to claim 1, is characterized in that: ultrasonic treatment time 30min in step b.
5. the method extracting squid active peptide from squid according to claim 1, is characterized in that: ul-trasonic irradiation temperature 60 C in step b.
6. the method extracting squid active peptide from squid according to claim 1, is characterized in that: ultrasonic power 300W in step b.
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| CN103947818B true CN103947818B (en) | 2016-01-20 |
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| CN104705479A (en) * | 2015-03-31 | 2015-06-17 | 广西还珠海洋生物科技有限公司 | Squid active peptide extracting method |
| CN105925649B (en) * | 2016-05-31 | 2020-01-07 | 舟山赛莱特海洋科技有限公司 | Preparation method of low-molecular-weight degreased squid protein functional active peptide |
| CN113439849B (en) * | 2021-06-28 | 2022-06-21 | 华南理工大学 | A marine fish peptide containing rhizoma Alpiniae Officinarum and its preparation method |
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| CN1628540A (en) * | 2003-12-16 | 2005-06-22 | 威海市宇王水产有限公司 | Production method of fish scale collagen |
| CN101003827A (en) * | 2007-01-09 | 2007-07-25 | 中国海洋大学 | Method for preparing bioactive peptide of collagen from squid skin, and application |
| CN102217699A (en) * | 2010-04-19 | 2011-10-19 | 浙江海洋学院 | Preparation method of squid skin protein active peptide |
| CN103386144A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
| CN103627763A (en) * | 2013-11-14 | 2014-03-12 | 浙江省海洋开发研究院 | Method for grading molecular weight of anglerfish skin collagen peptide |
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2014
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628540A (en) * | 2003-12-16 | 2005-06-22 | 威海市宇王水产有限公司 | Production method of fish scale collagen |
| CN101003827A (en) * | 2007-01-09 | 2007-07-25 | 中国海洋大学 | Method for preparing bioactive peptide of collagen from squid skin, and application |
| CN102217699A (en) * | 2010-04-19 | 2011-10-19 | 浙江海洋学院 | Preparation method of squid skin protein active peptide |
| CN103386144A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
| CN103627763A (en) * | 2013-11-14 | 2014-03-12 | 浙江省海洋开发研究院 | Method for grading molecular weight of anglerfish skin collagen peptide |
Non-Patent Citations (1)
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| 鱼类胶原蛋白及胶原活性多肽的研究进展;郭玉华等;《中国食品添加剂》;20101231(第3期);第175-179页 * |
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