CN108396054A - A method of enzymolysis Antarctic Ice fish prepares oligopeptide - Google Patents
A method of enzymolysis Antarctic Ice fish prepares oligopeptide Download PDFInfo
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- CN108396054A CN108396054A CN201810295542.4A CN201810295542A CN108396054A CN 108396054 A CN108396054 A CN 108396054A CN 201810295542 A CN201810295542 A CN 201810295542A CN 108396054 A CN108396054 A CN 108396054A
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- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The invention discloses a kind of methods that enzymolysis Antarctic Ice fish prepares oligopeptide, it is related to technical field of bioengineering, the method that enzymolysis Antarctic Ice fish prepares oligopeptide includes pretreatment of raw material process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；Pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；It is compound protease to digest the enzyme used in process；Rotary process includes primary centrifugation and secondary centrifuging；Filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter.High molecular weight protein in the Antarctic Ice fish of deep-sea is converted to small molecule oligopeptide by the present invention by biological enzymolysis technology, and the Antarctic Ice fish oligopeptide color of preparation is white or off-white color, and protein content is up to 95% or more, protein content is high, molecular weight is small, is easily absorbed by the human body utilization, and nutritive value is high.
Description
Technical field
The present invention relates to technical field of bioengineering, more particularly to a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide.
Background technology
Antarctic Ice fish is the higher fish of protein content having been found that, is grown in the pollution-free water of deep-sea subzero
Domain is mainly food with euphausiid shrimp, and protein content is rich in a variety of ammonia necessary to human body by dry weight up to 54% or more
Base acid.Essential amino acid contained in Antarctic Ice fish accounts for the 43.76% of its total amino acid content, it is necessary to amino acid with it is non-must
It is 77.81% to need the ratio of amino acid, this ratio meets the ideal protein pattern of FAO/WHO recommendations, and nutritive value is high.
Although the protein of Antarctic Ice fish is of great advantage to health, protein is as macromolecular substances, human body pair
Its absorption rate is relatively low, influences the performance of its nutritive value.Scientific research has confirmed that protein is mainly with oligomeric
The form of peptide is absorbed by the body, and then physiological function activity is played in human body, improves function of human body.
Currently, ocean fish oligopeptide type in the market is various, but Antarctic Ice fish polypeptide do not appear in also in the market, for
Deep-sea Antarctic Ice fish, also mainly be processed in the form of can or other ready-to-eat foods it is edible, process be in just
The grade stage there are no the report that field of food is used for about Antarctic Ice milt deep processing.
Invention content
According to technological deficiency existing in the prior art, the technical problem to be solved by the present invention is to：A kind of enzymolysis is provided
The method that Antarctic Ice fish prepares oligopeptide, by Antarctic Ice fish raw material digest etc. process obtain Antarctic Ice fish prepare it is low
Poly- peptide, Antarctic Ice fish oligopeptide prepared by the present invention, protein content is high, and molecular weight is small, is easily absorbed by the human body utilization, nutritive value
It is high.
In order to realize that the technical purpose of the present invention, a kind of enzymolysis Antarctic Ice fish of the present invention prepare the technology of the method for oligopeptide
Scheme is：
A method of enzymolysis Antarctic Ice fish prepares oligopeptide, and the method that the enzymolysis Antarctic Ice fish prepares oligopeptide includes raw material
Pretreatment process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
The pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Enzyme used in the enzymolysis process is specific proteases, and hydrolysis temperature is 50-55 DEG C, enzymolysis time 4-6h；
The centrifugal speed of the rotary process is 6000-8000r/min, centrifugation time 8-10min；
The filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter；
The thickening temperature of the enrichment process is 60-80 DEG C, a concentration of 20-25% of Baume being finally concentrated to；
The intake air temperature of the spray drying process is 160-180 DEG C, and air outlet temperature is 70-90 DEG C.
As a further improvement on the present invention, the raw material cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning.
As a further improvement on the present invention, the ultrasonic power of the ultrasonic cleaning is 150-200W, ultrasonic time 20-
30min。
As a further improvement on the present invention, the meat grinder grate aperture used in the pulverizing process is 5-8mm.
As a further improvement on the present invention, the boiling temperature of the boiling sterilization process is 110-120 DEG C, digestion time
For 10-20min.
As a further improvement on the present invention, the specific proteases are complex enzyme.
As a further improvement on the present invention, the specific proteases are alkali protease, trypsase and neutral protein
The complex enzyme of enzyme.
As a further improvement on the present invention, the rotary process includes primary centrifugation and secondary centrifuging.
Compared with prior art, the beneficial effects of the invention are as follows：
1, the high molecular weight protein in the Antarctic Ice fish of deep-sea is converted to small molecule oligopeptide, institute by the present invention by biological enzymolysis technology
Oligopeptide obtained have it is pure natural, without any adding ingredient, the advantages such as protein content is high, molecular weight is small, is easy to absorb, through spy
Product features good taste, fishy smell are small after different process, are easy to receive in mouthfeel, can be added to various food, health products and cosmetics
In, nutritive value is high, has a wide range of application.
2, the Antarctic Ice fish oligopeptide of preparation of the invention, color are white or off-white color, protein content up to 95% with
On.
3, the present invention handles the free of contamination Antarctic Ice fish in deep-sea through modern biotechnology zymolysis technique, and it is oligomeric to be prepared into small molecule
Peptide is easily absorbed by the human body, and does not increase stomach and intestine burden, meanwhile, the processing method for having deepened Antarctic Ice fish is further enriched, is improved
Value-added content of product and nutritive value.
4, the oligopeptide that the present invention obtains, no any other ektogenic addition, belongs to natural food, through multiple not Tongfang
After formula filtering, obtained product purity higher, and be also easier to be received on taste.
5, the oligopeptide that the present invention obtains, has a wide range of application, application form is various, can be added to various food, health products
And in cosmetics, as one kind of marine fishes oligopeptide, there is good health-care effect to health.
Specific implementation mode
To be further elaborated to advantageous effect of the present invention, a large number of experiments, special instruction, present invention experiment have been carried out
It is intended to illustrate the advantageous effect of inventive technique, is not restricted to the scope of the present invention.
Embodiment 1
A method of enzymolysis Antarctic Ice fish prepares oligopeptide, and the method that the enzymolysis Antarctic Ice fish prepares oligopeptide includes raw material
Pretreatment process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
Pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Raw material cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning；
The ultrasonic power of ultrasonic cleaning is 150-200W, ultrasonic time 20-30min.
Ice fish after fresh or defrosting is put into supersonic wave cleaning machine by ultrasonic cleaning process, setting ultrasonic power 150-
200w, ultrasonic time 20-30min are fully washed off the mucus on ice fish surface and the impurity dirt in scale, are then set using special
It is standby to go end to end and internal organ.
Ice fish after decaptitating and internal organ is put into purge tank and cleans by secondary cleaning process, setting cleaning duration 15-
20min washes off the impurity such as blood stains, spare.
Meat grinder grate aperture used in pulverizing process is 5-8mm.
Pulverizing process includes Minced Steak and homogenate, the Antarctic Ice fish handled well is rubbed with meat grinder, meat grinder grate aperture
For 5-8mm, later according to 1：The ratio of 1-5 adds water to be homogenized, spare.
The boiling temperature of boiling sterilization process is 110-120 DEG C, digestion time 10-20min.
The Antarctic Ice fish mixed liquor being homogenized is poured into cooker, sets boiling temperature as 110-120 by boiling sterilization process
DEG C, maintain 10-20min, boiling to achieve the purpose that sterilizing while leaching albumen, then meat soup is put into meat soup storage tank and is stored up
It deposits.
It is specific proteases to digest the enzyme used in process, and hydrolysis temperature is 50-55 DEG C, enzymolysis time 4-6h；
Specific proteases are complex enzyme；
Specific proteases are the complex enzyme of alkali protease, trypsase and neutral proteinase.
Soup in meat soup storage tank is directly discharged into hydrolysis device by enzymolysis process, sets hydrolysis temperature as 50-55 DEG C, and
PH is adjusted with sodium hydroxide, a certain amount of alkali protease, trypsase and neutral proteinase complex enzyme is added again by raw meat
It is digested, enzymolysis time 4-6h, constantly adjusts pH in enzymolysis process, ensure the enzymatic hydrolysis condition of enzymatic hydrolysis system.After the completion of enzymolysis,
Enzyme deactivation sets 90-95 DEG C of enzyme-removal temperature, time 5-10min.
The centrifugal speed of rotary process is 6000-8000r/min, centrifugation time 8-10min；
Rotary process includes primary centrifugation and secondary centrifuging.
Hydrolyzate after enzyme deactivation is discharged into centrifugal device and centrifuged by centrifugal process, setting centrifugal speed 6000-
8000r/min, centrifugation time 8-10min, deslagging, bone slag and meat slag in this step can be recycled when extracting again.
Supernatant after centrifugal filtration is placed in centrifugal device by secondary centrifuging process, carries out secondary centrifuging, is realized
The separation of fat and the stillness of night sets centrifugal speed 6000-8000r/min, centrifugation time 8-10min.
Filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter.
Investigation on Plate Filtration is filtered using plate-frame filtering device to clear by the hydrolyzate after centrifugation after decolorizing with activated carbon
Night storage device determines the need for circulating filtration by clarity of solution.
Ultrafiltration and sodium filter journey, and the stillness of night after plate-frame filtering is discharged into ultrafiltration apparatus, and ultrafiltration is carried out using ultrafiltration membrane, then
Pass through sodium successively and filter equipment, finally obtains the high polypeptide solution of purity.
The thickening temperature of enrichment process is 60-80 DEG C, a concentration of 20-25% of Baume being finally concentrated to.
Solution concentration process concentrates the high polypeptide solution of purity, sets thickening temperature as 60-80 DEG C, final dense
It is reduced to a concentration of 20-25% of Baume.
The intake air temperature of spray drying process is 160-180 DEG C, and air outlet temperature is 70-90 DEG C.
Concentrate is made ice fish polypeptide by spray-drying installation, sets spray-drying installation by spray-drying process
Intake air temperature is 160-180 DEG C, and air outlet temperature is 70-90 DEG C.
The present invention preparation Antarctic Ice fish oligopeptide, color be white or off-white color, protein content up to 95% with
On.
Embodiment 2
A method of enzymolysis Antarctic Ice fish prepares oligopeptide, and the method that the enzymolysis Antarctic Ice fish prepares oligopeptide includes raw material
Pretreatment process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
Pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Raw material cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning；
The ultrasonic power of ultrasonic cleaning is 150W, ultrasonic time 20min.
Ice fish after fresh or defrosting is put into supersonic wave cleaning machine, sets ultrasonic power 150w by ultrasonic cleaning process,
Ultrasonic time 20min is fully washed off the mucus on ice fish surface and the impurity dirt in scale, is then gone end to end using special equipment
And internal organ.
Ice fish after decaptitating and internal organ is put into purge tank and cleans by secondary cleaning process, setting cleaning duration
15min washes off the impurity such as blood stains, spare.
Meat grinder grate aperture used in pulverizing process is 5mm.
Pulverizing process includes Minced Steak and homogenate, the Antarctic Ice fish handled well is rubbed with meat grinder, meat grinder grate aperture
For 5mm, later according to 1：1 ratio adds water to be homogenized, spare.
The boiling temperature of boiling sterilization process is 110 DEG C, digestion time 10min.
The Antarctic Ice fish mixed liquor being homogenized is poured into cooker by boiling sterilization process, sets boiling temperature as 110 DEG C,
10min, boiling is maintained to achieve the purpose that sterilizing while leaching albumen, then meat soup is put into meat soup storage tank and is stored.
It is specific proteases to digest the enzyme used in process, and hydrolysis temperature is 50 DEG C, enzymolysis time 4h；
Specific proteases are complex enzyme；
Specific proteases are the complex enzyme of alkali protease, trypsase and neutral proteinase.
Soup in meat soup storage tank is directly discharged into hydrolysis device by enzymolysis process, sets hydrolysis temperature as 50 DEG C, hydrogen is used in combination
Sodium oxide molybdena adjusts pH, and a certain amount of alkali protease, trypsase and neutral proteinase complex enzyme is added again by raw meat and carries out
It digests, enzymolysis time 4h, constantly adjusts pH in enzymolysis process, ensure the enzymatic hydrolysis condition of enzymatic hydrolysis system.After the completion of enzymolysis, enzyme deactivation,
Set 90 DEG C of enzyme-removal temperature, time 5min.
The centrifugal speed of rotary process is 6000r/min, centrifugation time 8min；
Rotary process includes primary centrifugation and secondary centrifuging.
Hydrolyzate after enzyme deactivation is discharged into centrifugal device and centrifuged by centrifugal process, setting centrifugal speed 6000r/
Min, centrifugation time 8min, deslagging, bone slag and meat slag in this step can be recycled when extracting again.
Supernatant after centrifugal filtration is placed in centrifugal device by secondary centrifuging process, carries out secondary centrifuging, is realized
The separation of fat and the stillness of night sets centrifugal speed 6000r/min, centrifugation time 8min.
Filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter.
Investigation on Plate Filtration is filtered using plate-frame filtering device to clear by the hydrolyzate after centrifugation after decolorizing with activated carbon
Night storage device determines the need for circulating filtration by clarity of solution.
Ultrafiltration and sodium filter journey, and the stillness of night after plate-frame filtering is discharged into ultrafiltration apparatus, and ultrafiltration is carried out using ultrafiltration membrane, then
Pass through sodium successively and filter equipment, finally obtains the high polypeptide solution of purity.
The thickening temperature of enrichment process is 60 DEG C, the Baume a concentration of 20% being finally concentrated to.
Solution concentration process concentrates the high polypeptide solution of purity, sets thickening temperature as 60 DEG C, is finally concentrated to
Baume a concentration of 20%.
The intake air temperature of spray drying process is 160 DEG C, and air outlet temperature is 70 DEG C.
Concentrate is made ice fish polypeptide by spray-drying installation, sets spray-drying installation by spray-drying process
Intake air temperature is 160 DEG C, and air outlet temperature is 70 DEG C.
The Antarctic Ice fish oligopeptide of the preparation of the present invention, color are white or off-white color, protein content 95.25%.
Embodiment 3
A method of enzymolysis Antarctic Ice fish prepares oligopeptide, and the method that the enzymolysis Antarctic Ice fish prepares oligopeptide includes raw material
Pretreatment process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
Pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Raw material cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning；
The ultrasonic power of ultrasonic cleaning is 170W, ultrasonic time 25min.
Ice fish after fresh or defrosting is put into supersonic wave cleaning machine, sets ultrasonic power 170w by ultrasonic cleaning process,
Ultrasonic time 25min is fully washed off the mucus on ice fish surface and the impurity dirt in scale, is then gone end to end using special equipment
And internal organ.
Ice fish after decaptitating and internal organ is put into purge tank and cleans by secondary cleaning process, setting cleaning duration
18min washes off the impurity such as blood stains, spare.
Meat grinder grate aperture used in pulverizing process is 7mm.
Pulverizing process includes Minced Steak and homogenate, the Antarctic Ice fish handled well is rubbed with meat grinder, meat grinder grate aperture
For 7mm, later according to 1：4 ratio adds water to be homogenized, spare.
The boiling temperature of boiling sterilization process is 115 DEG C, digestion time 15min.
The Antarctic Ice fish mixed liquor being homogenized is poured into cooker by boiling sterilization process, sets boiling temperature as 115 DEG C,
15min, boiling is maintained to achieve the purpose that sterilizing while leaching albumen, then meat soup is put into meat soup storage tank and is stored.
It is specific proteases to digest the enzyme used in process, and hydrolysis temperature is 53 DEG C, enzymolysis time 5h；
Specific proteases are complex enzyme；
Specific proteases are the complex enzyme of alkali protease, trypsase and neutral proteinase.
Soup in meat soup storage tank is directly discharged into hydrolysis device by enzymolysis process, sets hydrolysis temperature as 53 DEG C, hydrogen is used in combination
Sodium oxide molybdena adjusts pH, and a certain amount of alkali protease, trypsase and neutral proteinase complex enzyme is added again by raw meat and carries out
It digests, enzymolysis time 5h, constantly adjusts pH in enzymolysis process, ensure the enzymatic hydrolysis condition of enzymatic hydrolysis system.After the completion of enzymolysis, enzyme deactivation,
Set 93 DEG C of enzyme-removal temperature, time 8min.
The centrifugal speed of rotary process is 7000r/min, centrifugation time 9min；
Rotary process includes primary centrifugation and secondary centrifuging.
Hydrolyzate after enzyme deactivation is discharged into centrifugal device and centrifuged by centrifugal process, setting centrifugal speed 7000r/
Min, centrifugation time 9min, deslagging, bone slag and meat slag in this step can be recycled when extracting again.
Supernatant after centrifugal filtration is placed in centrifugal device by secondary centrifuging process, carries out secondary centrifuging, is realized
The separation of fat and the stillness of night sets centrifugal speed 7000r/min, centrifugation time 9min.
Filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter.
Investigation on Plate Filtration is filtered using plate-frame filtering device to clear by the hydrolyzate after centrifugation after decolorizing with activated carbon
Night storage device determines the need for circulating filtration by clarity of solution.
Ultrafiltration and sodium filter journey, and the stillness of night after plate-frame filtering is discharged into ultrafiltration apparatus, and ultrafiltration is carried out using ultrafiltration membrane, then
Pass through sodium successively and filter equipment, finally obtains the high polypeptide solution of purity.
The thickening temperature of enrichment process is 70 DEG C, the Baume a concentration of 23% being finally concentrated to.
Solution concentration process concentrates the high polypeptide solution of purity, sets thickening temperature as 70 DEG C, is finally concentrated to
Baume a concentration of 23%.
The intake air temperature of spray drying process is 170 DEG C, and air outlet temperature is 80 DEG C.
Concentrate is made ice fish polypeptide by spray-drying installation, sets spray-drying installation by spray-drying process
Intake air temperature is 170 DEG C, and air outlet temperature is 80 DEG C.
The Antarctic Ice fish oligopeptide of the preparation of the present invention, color are white or off-white color, protein content 95.45%.
Embodiment 4
A method of enzymolysis Antarctic Ice fish prepares oligopeptide, and the method that the enzymolysis Antarctic Ice fish prepares oligopeptide includes raw material
Pretreatment process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
Pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Raw material cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning；
The ultrasonic power of ultrasonic cleaning is 180W, ultrasonic time 25min.
Ice fish after fresh or defrosting is put into supersonic wave cleaning machine, sets ultrasonic power 180w by ultrasonic cleaning process,
Ultrasonic time 25min is fully washed off the mucus on ice fish surface and the impurity dirt in scale, is then gone end to end using special equipment
And internal organ.
Ice fish after decaptitating and internal organ is put into purge tank and cleans by secondary cleaning process, setting cleaning duration
18min washes off the impurity such as blood stains, spare.
Meat grinder grate aperture used in pulverizing process is 5mm.
Pulverizing process includes Minced Steak and homogenate, the Antarctic Ice fish handled well is rubbed with meat grinder, meat grinder grate aperture
For 5mm, later according to 1：3 ratio adds water to be homogenized, spare.
The boiling temperature of boiling sterilization process is 110 DEG C, digestion time 20min.
The Antarctic Ice fish mixed liquor being homogenized is poured into cooker, sets boiling temperature as 110-120 by boiling sterilization process
DEG C, maintain 10-20min, boiling to achieve the purpose that sterilizing while leaching albumen, then meat soup is put into meat soup storage tank and is stored up
It deposits.
It is specific proteases to digest the enzyme used in process, and hydrolysis temperature is 50-55 DEG C, enzymolysis time 4-6h；
Specific proteases are complex enzyme；
Specific proteases are the complex enzyme of alkali protease, trypsase and neutral proteinase.
Soup in meat soup storage tank is directly discharged into hydrolysis device by enzymolysis process, sets hydrolysis temperature as 53 DEG C, hydrogen is used in combination
Sodium oxide molybdena adjusts pH, and a certain amount of alkali protease, trypsase and neutral proteinase complex enzyme is added again by raw meat and carries out
It digests, enzymolysis time 6h, constantly adjusts pH in enzymolysis process, ensure the enzymatic hydrolysis condition of enzymatic hydrolysis system.After the completion of enzymolysis, enzyme deactivation,
Set 95 DEG C of enzyme-removal temperature, time 8min.
The centrifugal speed of rotary process is 6000r/min, centrifugation time 6-8min；
Rotary process includes primary centrifugation and secondary centrifuging.
Hydrolyzate after enzyme deactivation is discharged into centrifugal device and centrifuged by centrifugal process, setting centrifugal speed 6000r/
Min, centrifugation time 8min, deslagging, bone slag and meat slag in this step can be recycled when extracting again.
Supernatant after centrifugal filtration is placed in centrifugal device by secondary centrifuging process, carries out secondary centrifuging, is realized
The separation of fat and the stillness of night sets centrifugal speed 6000r/min, centrifugation time 6min.
Filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter.
Investigation on Plate Filtration is filtered using plate-frame filtering device to clear by the hydrolyzate after centrifugation after decolorizing with activated carbon
Night storage device determines the need for circulating filtration by clarity of solution.
Ultrafiltration and sodium filter journey, and the stillness of night after plate-frame filtering is discharged into ultrafiltration apparatus, and ultrafiltration is carried out using ultrafiltration membrane, then
Pass through sodium successively and filter equipment, finally obtains the high polypeptide solution of purity.
The thickening temperature of enrichment process is 70 DEG C, the Baume a concentration of 25% being finally concentrated to.
Solution concentration process concentrates the high polypeptide solution of purity, sets thickening temperature as 70 DEG C, is finally concentrated to
Baume a concentration of 25%.
The intake air temperature of spray drying process is 180 DEG C, and air outlet temperature is 80 DEG C.
Concentrate is made ice fish polypeptide by spray-drying installation, sets spray-drying installation by spray-drying process
Intake air temperature is 180 DEG C, and air outlet temperature is 80 DEG C.
The Antarctic Ice fish oligopeptide of the preparation of the present invention, color are white or off-white color, protein content 96.25%.
Embodiment 5
A method of enzymolysis Antarctic Ice fish prepares oligopeptide, and the method that the enzymolysis Antarctic Ice fish prepares oligopeptide includes raw material
Pretreatment process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
Pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Raw material cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning；
The ultrasonic power of ultrasonic cleaning is 200W, ultrasonic time 30min.
Ice fish after fresh or defrosting is put into supersonic wave cleaning machine, sets ultrasonic power 200w by ultrasonic cleaning process,
Ultrasonic time 30min is fully washed off the mucus on ice fish surface and the impurity dirt in scale, is then gone end to end using special equipment
And internal organ.
Ice fish after decaptitating and internal organ is put into purge tank and cleans by secondary cleaning process, setting cleaning duration
20min washes off the impurity such as blood stains, spare.
Meat grinder grate aperture used in pulverizing process is 8mm.
Pulverizing process includes Minced Steak and homogenate, the Antarctic Ice fish handled well is rubbed with meat grinder, meat grinder grate aperture
For 8mm, later according to 1：5 ratio adds water to be homogenized, spare.
The boiling temperature of boiling sterilization process is 120 DEG C, digestion time 20min.
The Antarctic Ice fish mixed liquor being homogenized is poured into cooker by boiling sterilization process, sets boiling temperature as 120 DEG C,
20min, boiling is maintained to achieve the purpose that sterilizing while leaching albumen, then meat soup is put into meat soup storage tank and is stored.
It is specific proteases to digest the enzyme used in process, and hydrolysis temperature is 55 DEG C, enzymolysis time 6h；
Specific proteases are complex enzyme；
Specific proteases are the complex enzyme of alkali protease, trypsase and neutral proteinase.
Soup in meat soup storage tank is directly discharged into hydrolysis device by enzymolysis process, sets hydrolysis temperature as 55 DEG C, hydrogen is used in combination
Sodium oxide molybdena adjusts pH, and a certain amount of alkali protease, trypsase and neutral proteinase complex enzyme is added again by raw meat and carries out
It digests, enzymolysis time 6h, constantly adjusts pH in enzymolysis process, ensure the enzymatic hydrolysis condition of enzymatic hydrolysis system.After the completion of enzymolysis, enzyme deactivation,
Set 95 DEG C of enzyme-removal temperature, time 10min.
The centrifugal speed of rotary process is 8000r/min, centrifugation time 10min；
Rotary process includes primary centrifugation and secondary centrifuging.
Hydrolyzate after enzyme deactivation is discharged into centrifugal device and centrifuged by centrifugal process, setting centrifugal speed 8000r/
Min, centrifugation time 10min, deslagging, bone slag and meat slag in this step can be recycled when extracting again.
Supernatant after centrifugal filtration is placed in centrifugal device by secondary centrifuging process, carries out secondary centrifuging, is realized
The separation of fat and the stillness of night sets centrifugal speed 8000r/min, centrifugation time 10min.
Filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter.
Investigation on Plate Filtration is filtered using plate-frame filtering device to clear by the hydrolyzate after centrifugation after decolorizing with activated carbon
Night storage device determines the need for circulating filtration by clarity of solution.
Ultrafiltration and sodium filter journey, and the stillness of night after plate-frame filtering is discharged into ultrafiltration apparatus, and ultrafiltration is carried out using ultrafiltration membrane, then
Pass through sodium successively and filter equipment, finally obtains the high polypeptide solution of purity.
The thickening temperature of enrichment process is 80 DEG C, the Baume a concentration of 25% being finally concentrated to.
Solution concentration process concentrates the high polypeptide solution of purity, sets thickening temperature as 80 DEG C, is finally concentrated to
Baume a concentration of 25%.
The intake air temperature of spray drying process is 180 DEG C, and air outlet temperature is 90 DEG C.
Concentrate is made ice fish polypeptide by spray-drying installation, sets spray-drying installation by spray-drying process
Intake air temperature is 180 DEG C, and air outlet temperature is 90 DEG C.
The Antarctic Ice fish oligopeptide of the preparation of the present invention, color are white or off-white color, protein content 95.86%.
The above is only the preferable embodiment of the present invention, is not intended to limit the present invention in any form, Ren Hewei
It is detached from technical solution of the present invention content, according to the technical essence of the invention to any simple modification, transformation made by above example
Material equivalent variations and modification, in the range of still falling within technical solution of the present invention.
Claims (8)
1. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide, it is characterised in that：The enzymolysis Antarctic Ice fish prepares oligopeptide
Method include pretreatment of raw material process, enzymolysis process, rotary process, filter progress, enrichment process and spray drying process；
The pretreatment of raw material process includes raw material cleaning, go end to end, remove internal organ, drain, crushing, the processes such as boiling sterilization；
Enzyme used in the enzymolysis process is specific proteases, and hydrolysis temperature is 50-55 DEG C, enzymolysis time 4-6h；
The centrifugal speed of the rotary process is 6000-8000r/min, centrifugation time 8-10min；
The filter progress includes activated carbon decolorizing, plate-frame filtering, ultrafiltration and sodium filter；
The thickening temperature of the enrichment process is 60-80 DEG C, a concentration of 20-25% of Baume being finally concentrated to；
The intake air temperature of the spray drying process is 160-180 DEG C, and air outlet temperature is 70-90 DEG C.
2. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide according to claim 1, it is characterised in that：The raw material
Cleaning includes being cleaned by ultrasonic and purge tank secondary cleaning.
3. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide according to claim 2, it is characterised in that：The ultrasound
The ultrasonic power of cleaning is 150-200W, ultrasonic time 20-30min.
4. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide according to claim 3, it is characterised in that：The crushing
Meat grinder grate aperture used in process is 5-8mm.
5. the method that a kind of enzymolysis Antarctic Ice fish according to Claims 1-4 any claim prepares oligopeptide, special
Sign is：The boiling temperature of the boiling sterilization process is 110-120 DEG C, digestion time 10-20min.
6. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide according to claim 1, it is characterised in that：It is described specific
Protease is complex enzyme.
7. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide according to claim 6, it is characterised in that：It is described specific
Protease is the complex enzyme of alkali protease, trypsase and neutral proteinase.
8. a kind of method that enzymolysis Antarctic Ice fish prepares oligopeptide according to claim 1, it is characterised in that：The centrifugation
Process includes primary centrifugation and secondary centrifuging.
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