CN104232715A - Preparation method of protein oligopeptide - Google Patents
Preparation method of protein oligopeptide Download PDFInfo
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- CN104232715A CN104232715A CN201410410831.6A CN201410410831A CN104232715A CN 104232715 A CN104232715 A CN 104232715A CN 201410410831 A CN201410410831 A CN 201410410831A CN 104232715 A CN104232715 A CN 104232715A
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Abstract
The invention discloses a preparation method of a protein oligopeptide and particularly relates to a method of obtaining the protein oligopeptide by using chicken, fish meat or bone-containing chicken and fish meat as a basic raw material by means of enzymolysis of compound protease. The method comprises the following steps: firstly, cleaning and crushing bone-containing chicken and fish meat tissues; adding water into a reaction kettle and heating to 100 DEG C for protein denaturation; cooling and adding the compound protease; controlling the temperature and the pH value, and carrying out enzymolysis while stirring; filtering and centrifugalizing hydrolysate to obtain a clarified aqueous phase liquid; and decoloring active carbon and debitterizing, desalting, concentrating, spraying and drying to obtain the protein oligopeptide. According to the preparation method disclosed by the invention, processing wastes of chicken, fish meat or bone-containing chicken and fish meat are used as basic materials, so that the preparation method is easily available in used raw materials, low in cost, high in additional value, simple in technical process and easy to control hydrolytic process, and the obtained product is good in quality and high in safety and is expected to be used for medicine raw materials and drugs and the like.
Description
Technical field
The present invention relates to animal proteinum processing technique field, particularly one utilizes enzymolysis process to decompose animal proteinum, and extracts the method for albumen oligopeptide from enzyme resolvent.
Background technology
Amino acid is the basic comprising unit of protein.Different types of amino acid, according to specific order, forms the protein of structure difference, Various Functions.It is generally acknowledged that protein is by the Amino acid profile of more than 51.And in life entity, also having the multiple material by 2-50 Amino acid profile, scientific circles are referred to as " peptide ".Peptide is the material between amino acid and protein.Amino acid whose molecule is minimum, and protein is maximum, and two or more amino acid dehydrating condensations forms several peptide bonds thus a composition peptide, and multiple peptide carries out a multistage folding just composition protein molecule.Usually, will be called oligopeptide by 2-10 Amino acid profile peptide, what be made up of 11-50 amino acid is called polypeptide.On material is formed, peptide and protein have homogeneity, but there is the physiological function of the uniqueness being different from amino acid and protein, especially oligopeptide, there is the characteristics such as molecular weight is little, good, the direct absorptivity of good water solubility, Heat stability is good, acid acceptance, high safety.Be applied to the food and low irritability food, protective foods, sports food, high-protein food, bakery product, candy, cake, cold drink etc. with nutrition curative effect.
Rich in protein in chicken, the flesh of fish, amino acid composition comprehensively, is the animal protein food that people are desirable.China chicken, fishery product are at present based on fresh goods, and in converted products, primary processed items proportion is large, and intensive processing product is obviously not enough, low to the comprehensive utilization degree of agricultural resource.In order to make up the deficiency of traditional chicken, fish product deep exploitation aspect, the present invention adopts complex enzyme zymohydrolysis technology, to chicken, the flesh of fish or containing bone chicken, the flesh of fish carry out directional enzymatic, obtain oligopeptide.
Summary of the invention
A kind of biological enzyme hydrolysis method is the object of the present invention is to provide to decompose chicken, fish protein, produce the method for albumen oligopeptide, change the situation of current chicken, fish protein working depth deficiency, improve protein bio utilization ratio, expand egg albumen, fish-protein product applications.
For solving the problems of the technologies described above, the technical solution used in the present invention is a kind of preparation method of albumen oligopeptide, and its step comprises:
One, raw materials pretreatment and enzymolysis: muscle tissue pulverizing heating process, enzyme-added enzymolysis, deactivation, filtration, centrifugal treating obtain clarifying enzymolysis solution in a kettle..
Two, oligopeptide is refined: enzymolysis solution ultrafiltration, decolouring, debitterize, nanofiltration, spraying process, obtain the oligomeric Gly-His-Lys of albumen.
Preferably, a kind of albumen oligopeptide preparation method described in step one, after it is characterized in that the muscle tissue cleaning described in step (1), simple crushing becomes 5-20 ㎜ fragment, add quality 1-4 water mixing doubly and put into reactor, be heated to 100 DEG C and keep 1-2 hour, make protein denaturation, cold water cooling is passed in reacting kettle jacketing, when being 40-50 DEG C to reactor temperature, add the compound protease of raw material weight 0.1-0.3%, keep 40-60 DEG C of temperature, pH value 7-9, stir hydrolysis 2-12 hour.Solution after hydrolysis filters to obtain the smart filtrate of clarification through filter-press essence, detects OD
550transmittance meets >50% requirement.Essence filtrate uses remaining grease and the insoluble impurity of solid in three phase centrifugation device processes removing solution, obtains clarification aqueous phase liquid.
Preferably, a kind of albumen oligopeptide preparation method described in step 2, it is characterized in that the ultrafiltration apparatus circular treatment of the aqueous phase liquid use molecular weight cut-off 5-10KD described in step (2), obtain concentrated solution and permeate, after material liquid volume reduces, can add further in concentrated solution purified water continue ultrafiltration, with improve oligopeptide through efficiency, the permeate obtained is albumen oligopeptide solution.In permeate, drop into the food grade gac of 0.1-0.6%, adjust ph 6-7.5, carry out debitterize, decolouring, cross the carbon dust filtered in feed liquid, obtain the albumen oligopeptide liquid of transparent color and luster clarification.The nanofiltration equipment of molecular weight cut-off 500-1000D is used to carry out desalting treatment through the clear oligopeptide liquid that debitterize, desolventing technology are good, as detected the soluble salt too high levels in feed liquid, can add purified water nanofiltration repeatedly in feed liquid, the trapped fluid that nanofiltration obtains is the oligopeptide liquid after desalting treatment.Use vacuum distillation equipment that oligopeptide liquid is concentrated into solid content 30-50%, under 60-70 DEG C of condition, insulation is preserved, and use spray drying device to carry out spraying dry, inlet temperature controls at 140-160 DEG C, air outlet temperature controls at 60-100 DEG C, obtains the oligomeric Gly-His-Lys of finished product albumen.
The albumen oligopeptide using the inventive method to extract from chicken, structure of fish muscle, can be applied in food, beverage and various healthcare products.The product depth that the invention solves traditional technology existence is not high, and the drawback that Application Areas is limited, effectively improves the bioavailability of protein product, and the theoretical yield of protein is 100%, can form good economic and social benefit.
Embodiment
Describe embodiments of the present invention in detail below with reference to embodiment, to the present invention, how utilisation technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1:
By 1 ton of fresh chicken, simply rinse containing bone chicken, be broken into the fragment of 5mm, add in reactor after mixing with 2 tons of water, be heated to 100 DEG C keep 1.5 hours, ensure that cube meat boils as degree, in reacting kettle jacketing, then pass into cold water cooling, when being cooled to 45 DEG C, add 1kg compound protease (wherein, pancreatin 625 g, Sumizyme MP 375 g), keeps 55 DEG C, pH value 7-9, stirs hydrolysis 10 hours.Solution after hydrolysis filters to obtain the smart filtrate of clarification through filter-press essence, detects OD
550transmittance meets >50% requirement.Essence filtrate uses remaining grease and the insoluble impurity of solid in three phase centrifugation device processes removing solution, obtains clarification aqueous phase liquid.Aqueous phase liquid uses the ultrafiltration apparatus circular treatment of molecular weight cut-off 5-10KD, obtain concentrated solution and permeate, the food grade gac of 0.1-0.6% is dropped in permeate, adjust ph 6.0-7.5, carry out debitterize, decolouring, cross the carbon dust filtered in feed liquid, obtain the albumen oligopeptide liquid of transparent color and luster clarification.Use the nanofiltration equipment of molecular weight cut-off 500-1000D to carry out desalting treatment through the clear oligopeptide liquid that debitterize, desolventing technology are good, the trapped fluid that nanofiltration obtains is the oligopeptide liquid after desalting treatment.Use vacuum distillation equipment that oligopeptide liquid is concentrated into solid content 30-50%, under 65 DEG C of conditions, insulation is preserved, spray drying device is used to carry out spraying dry, inlet temperature controls at 150-160 DEG C, air outlet temperature controls at 80-90 DEG C, obtains colourless, tasteless, less salt and molecular weight is albumen oligomeric Gly-His-Lys 150 Kg of 1-5KD.
Embodiment 2:
By 2 tons of fresh flesh of fish, rinse containing the bone flesh of fish is simple, be broken into the fragment of 10mm, add in reactor after mixing with 6 tons of water, be heated to 100 DEG C keep 2 hours, ensure that cube meat boils as degree, in reacting kettle jacketing, then pass into cold water cooling, be cooled to 50 DEG C, add 4kg compound protease (wherein, pancreatin 2.5 Kg, Sumizyme MP 1.5 Kg), keep 50 DEG C, adjust ph 7-9, stirs hydrolysis 8 hours.Solution after hydrolysis filters to obtain the smart filtrate of clarification through filter-press essence, detects OD
550transmittance meets >50% requirement.Essence filtrate uses remaining grease and the insoluble impurity of solid in three phase centrifugation device processes removing solution, obtains clarification aqueous phase liquid.Aqueous phase liquid uses the ultrafiltration apparatus circular treatment of molecular weight cut-off 5-10KD, obtain concentrated solution and permeate, the food grade gac of 0.1-0.6% is dropped in permeate, adjust ph 6.0-7.5, carry out debitterize, decolouring, cross the carbon dust filtered in feed liquid, obtain the albumen oligopeptide liquid of transparent color and luster clarification.Use the nanofiltration equipment of molecular weight cut-off 500-1000D to carry out desalting treatment through the clear oligopeptide liquid that debitterize, desolventing technology are good, the trapped fluid that nanofiltration obtains is the oligopeptide liquid after desalting treatment.Use vacuum distillation equipment that oligopeptide liquid is concentrated into solid content 30-50%, under 65 DEG C of conditions, insulation is preserved, spray drying device is used to carry out spraying dry, inlet temperature controls at 150-160 DEG C, air outlet temperature controls at 80-90 DEG C, obtains colourless, tasteless, less salt and molecular weight is albumen oligomeric Gly-His-Lys 300 Kg of 1-5 KD.
The above, be only the specific embodiment of the present invention, is not limited thereto, and is anyly familiar with those skilled in the art in the technical scope that the present invention discloses, and can expect change easily or replace, all should be encompassed within protection scope of the present invention.
Claims (3)
1. a preparation method for albumen oligopeptide, comprises the following steps:
(1) raw materials pretreatment and enzymolysis: muscle tissue pulverizing heating process, enzyme-added enzymolysis, deactivation, filtration, centrifugal treating obtain clarifying enzymolysis solution in a kettle., proteolytic enzyme used be pancreatin and Sumizyme MP prozyme in mass ratio 1:0.6 mix;
(2) oligopeptide is refined: enzymolysis solution ultrafiltration, decolouring, debitterize, nanofiltration, spraying process, obtain the oligomeric Gly-His-Lys of albumen.
2. the preparation method of a kind of albumen oligopeptide according to claim 1, after it is characterized in that chicken described in step (1) or structure of fish muscle cleaning, simple crushing becomes 5-20 ㎜ fragment, add quality 1-4 water mixing doubly and put into reactor, be heated to 100 DEG C and keep 1-2 hour, make protein denaturation, cold water cooling is passed in reacting kettle jacketing, when being 40-50 DEG C to reactor temperature, add the compound protease of raw material weight 0.1-0.3%, keep 40-60 DEG C of temperature, regulate pH value 7-9, stir hydrolysis 2-12 hour; Solution after hydrolysis filters to obtain the smart filtrate of clarification through filter-press essence, detects OD
550transmittance meets >50% requirement; Essence filtrate uses remaining grease and the insoluble impurity of solid in three phase centrifugation device processes removing solution, obtains clarification aqueous phase liquid.
3. the preparation method of a kind of albumen oligopeptide according to claim 1, it is characterized in that the ultrafiltration apparatus circular treatment of the aqueous phase liquid use molecular weight cut-off 5-10KD described in step (2), permeate is albumen oligopeptide solution; In permeate, drop into the food grade gac of 0.1-0.6%, adjust ph 6-7.5, carry out debitterize, desolventing technology, cross the carbon dust filtered in feed liquid, obtain the albumen oligopeptide liquid of transparent color and luster clarification; Continue to use the nanofiltration equipment of molecular weight cut-off 500-1000D to carry out desalting treatment, as detected the soluble salt too high levels in feed liquid, purified water nanofiltration repeatedly can be added in feed liquid, trapped fluid is the oligopeptide liquid after desalting treatment, use vacuum distillation equipment that oligopeptide liquid is concentrated into solid content 30-50%, under 60-70 DEG C of condition, insulation is preserved, and eventually passes spray drying step and obtains the oligomeric Gly-His-Lys of albumen, inlet temperature 140-160 DEG C, air outlet temperature 60-100 DEG C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105476030A (en) * | 2015-12-15 | 2016-04-13 | 北京天肽生物科技有限公司 | Multi-functional composite oligopeptide nutrition powder |
| CN105755081A (en) * | 2016-03-08 | 2016-07-13 | 海南亿肽生物科技有限公司 | Fish oligopeptide production process |
| CN106119328A (en) * | 2016-06-29 | 2016-11-16 | 大连深蓝肽科技研发有限公司 | A kind of high-quality shrimp oligopeptide powder, preparation method thereof of applicable industrialized production |
| CN108396054A (en) * | 2018-03-30 | 2018-08-14 | 山东诺贝肽生物科技有限公司 | A method of enzymolysis Antarctic Ice fish prepares oligopeptide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108396054A (en) * | 2018-03-30 | 2018-08-14 | 山东诺贝肽生物科技有限公司 | A method of enzymolysis Antarctic Ice fish prepares oligopeptide |
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Application publication date: 20141224 |