CN1966516A - Nanometre collagen oligopeptide and its producing method - Google Patents
Nanometre collagen oligopeptide and its producing method Download PDFInfo
- Publication number
- CN1966516A CN1966516A CNA2006100986529A CN200610098652A CN1966516A CN 1966516 A CN1966516 A CN 1966516A CN A2006100986529 A CNA2006100986529 A CN A2006100986529A CN 200610098652 A CN200610098652 A CN 200610098652A CN 1966516 A CN1966516 A CN 1966516A
- Authority
- CN
- China
- Prior art keywords
- collagen
- hydrolysis
- skin
- nanometre
- oligopeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Cosmetics (AREA)
Abstract
This invention relates to a one-step method for extracting nano-collagen oligopeptides from skin of animal directly. The said method includes enzymolyzing collagen protein-containing natural animal skin directly to obtain small molecule collagens with average molecular weight of about 500D and distributing mainly between 100-1000D, combination of dipeptides to heptapeptides. Molecules of Gaussian distribution are tripeptides, tetrapeptides and pentapeptides. Amino acid compositions of collagen are glycine 24%, praline 14%, hydroxyproline 11%, glutamic acid 9%, and arginine 8%. The extracted nano-collagen oligopeptides can be served as the raw materials of good medicine, health care products, and cosmetics.
Description
Technical field
The present invention relates to a kind of preparation method who directly extracts nanometre collagen oligopeptide from animal skin, is to contain the direct enzymolysis of natural animal skin of collagen protein specifically, obtains the small molecules nanometre collagen oligopeptide.The nanometre collagen oligopeptide that extracts with present method can be used as good medicine, healthcare products, cosmetic material.
Background technology
Collagen protein described in the present invention (Collagen) claim collagen again; it is animal connective tissue's important protein matter; reticular tissue is except containing 60～70% moisture; collagen protein has accounted for about 20～30%; because high-load collagen protein is arranged; reticular tissue has had certain structure and mechanics of machinery character, as the function to reach support, to protect such as tensile strength, pulling force, elastic force.Have about 16% to be protein in the human body component, collagen protein accounts for 30～40% of body internal protein total amount, in the human body 3kg left and right sides collagen protein is arranged approximately so grow up, mainly be present in integumentary musculature, bone, tooth, internal organ (as stomach, intestines, cardiopulmonary, blood vessel and esophagus) and eyes etc. and locate.
Except water, be made up of collagen protein by 70% in the skin components, skin tightly encases health everywhere as a big cover; surface-area is quite big, during the human limb activity, and collagen protein performance function in the skin; make skin have defencive function, suitable elasticity and flintiness are arranged again.Collagen protein in epidermal basement membrane and the corium is combined closely, and corium presents wavyly makes that also epidermis rises and falls thereupon.Trick energy free bend has elasticity during high jump, mainly give the credit to collagen protein.Collagen protein is hair root sebiferous gland and the cell that tightly encases in the skin corium in skin texture.Can follow the string behind the skin aging, main relevant with collagen protein.
Collagen protein is a main component of keeping the skin and the elasticity of muscle.But along with the age increases, it is that aged begins that the moisture in skin and the muscle can reduce this.At this moment, collagen fabric begins to become tiny, and the elasticity of elastin also can lower, and collagen protein and the disintegration gradually of the mutual well-regulated mesh structure of formation of elastin cause the generation of wrinkle at last in original corium.So replenishing collagen can make skin keep young, reason promptly is this.Add environmental pollution, uviolizing, a variety of causes such as stress, the result makes skin seem dry, and attenuation is followed the string, and wrinkle also increases gradually, the skin aging honorable reason that can lose one's bloom why that Here it is.
Therefore, skin enters aging time, and suitably replenishing collagen is very important.The collagen protein product usually is applied in the makeup to increase functions such as the preserving moisture of makeup, skin maintenance.
The common collagen protein of animal-origin is made up of three stranded spirane structures that form of a chain, every a chain is become by hundreds of to thousands of amino acid condensations, molecular-weight average is extremely millions of up to hundreds of thousands of, in the ordinary course of things, the common collagen protein of oral or external application, its bioavailability is very low.
Therefore, people become peptide to collagen hydrolysate by methods such as enzymes, to improve its bioavailability.Early stage and most Collagen Hydrolysates in the market, mainly constitute polyamino acid or be called collagen polypeptide by 30-100 amino acid, molecular-weight average reaches 1000-10000D, and its percutaneous permeability, bioavailability and effect increase than macromolecular collagen protein, but still limited.
Li Yan etc. following pair of enzyme synergetic hydrolysis pigskin of pH gradual change condition (" Food science ", 2003, V24, No7, P74-79) preparation collagen oligopeptide, the collagen oligopeptide that obtains be molecular weight distribution at 800～5000D, molecular-weight average is about 2500.
Zhao Zheng, Hu Aijun adopt the combinative enzyme hydrolysis fish-skin to prepare collagen oligopeptide in patent (CN200410071942), obtain molecular weight of product and are distributed in 2000～6000 scopes.
Old three, Li Xueqiangs etc. are in patent (CN02133825.6), and the employing gelatin is a raw material, and the preparation collagen polypeptide obtains the collagen polypeptide of molecular weight in 2000～8000D scope.
Shi Zongjie etc. are in patent (CN200310104047.4), and the employing pigskin is a raw material, and the combined-enzyme method hydrolysis obtains collagen polypeptide, and molecular weight is more than 5000D.
It is raw material that Guo Hongchen etc. (CN03110986.1) in patent adopt pigskin, and the combined-enzyme method hydrolysis obtains collagen polypeptide and contains about 10 amino acid, is the collagen polypeptide of molecular weight in 800～2000D scope.
Above-mentioned technology, though the Collagen Hydrolysate molecular weight of product is less relatively, percutaneous permeability is still not high, because molecular weight is still bigger, mainly on makeup play outer field nursing role, be difficult to penetrate into skin corium, really play nursing, the repair of skin.
Above-mentioned molecular weight of product still can not proper at last collagen oligopeptide (dipeptides is to seven peptides), does not therefore also have the value of collagen oligopeptide nutrition.
Summary of the invention
One of purpose of the present invention is by combined-enzyme method hydrolysis of animal skin, obtains nanometre collagen oligopeptide.
Another object of the present invention is that the nanometre collagen oligopeptide that hydrolysis obtains is used as medicine, healthcare products, cosmetic material.
A kind of nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, molecular weight mainly is distributed between 100～1000D, is the small molecules combination of dipeptides to seven peptides, and the Gaussian distribution peak molecular weight is about 500D;
A kind of nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin is mainly tripeptides, tetrapeptide, pentapeptide, its molecular size and length
Between several nanometers to tens nanometer;
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, comprise that the animal skin that will contain collagen protein is through pre-treatment, adopt the combined-enzyme method hydrolysis to extract collagen protein, macromolecular collagen protein is hydrolyzed into micromolecular collagen oligopeptide;
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, preprocessing process is after aquatic foods are raiseeed skin depilation, scrapes fat, cleans, pays and be broken into fritter, earlier with the saturated limewater immersion of 10 times of volumes 40 days, clean with water rinse, the water treatment animal skin;
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, the enzyme that hydrolysis is adopted is meant trypsinase, stomach en-, papoid, Sumizyme MP, matrix metalloproteinase, neutral protease, a kind or several combinations in the bromeline;
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, the consumption of every kind of enzyme is 0.5～2% of poultry tare weight amount, and the hydrolysis pH of every kind of enzyme is 3～10, and hydrolysis time is 1～8 hour.
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, the temperature of extracting nanometre collagen oligopeptide is 20-75 ℃;
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, the hydrolysis reaction that extracts nanometre collagen oligopeptide carries out under vacuum condition;
A kind of preparation method of the nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin, the hydrolysed mix solid content that extracts the nanometre collagen oligopeptide employing is 1-40%;
By the nanometre collagen oligopeptide of method for preparing gained, main as medicine, healthcare products, cosmetic material.
The collagen oligopeptide that obtains with the inventive method, characteristics with lower molecular weight, high biological activity, molecular weight mainly are distributed in 100～1000D, molecular-weight average 500D, its peptide chain length is about tens nanometers, be the collagen peptide molecule 1/10th, the thousandth of collagen molecules.
Collagen hydrolysate with the present invention obtains according to its molecular weight distribution, belongs to nanometre collagen oligopeptide, has extremely strong absorptivity, can pass through stomach and intestine, liver digestion, alleviates stomach and intestine, burden of liver greatly, improves bioavailability greatly; It is proteinic synthetic to participate in enzyme, hormone, needed by human body directly, plays a role and needn't resolve into amino acid again, and it is faster that it participates in collagen synthetic speed.
The nanometre collagen oligopeptide that obtains with the inventive method greatly improves the perviousness of skin, can enter skin corium and subcutis by epidermal barrier with speed infiltration faster, gives full play to the effect of its nutrition, maintenance skin.This is that common collagen protein and collagen peptide or polypeptide are difficult to reach.
The nanometre collagen oligopeptide that the inventive method obtains has significant biological activity, promotes inoblast, epithelial cell to form, and makes antibody, improves immunizing power, and its many physiologically actives are that amino acid, polypeptide, protein are unexistent.
The outstanding function performance of the nanometre collagen oligopeptide that obtains with the inventive method is:
(1), trophicity: the necessary nutrient of skin layer that can contain collagen protein, make active reinforcement of collagen protein in the skin, the integrity that keeps stratum corneum moisture and fibrous texture, improve the metabolism of skin cells living environment and promotion skin histology, increase circulation, reach the purpose of skin care delaying retrogradation, beauty treatment, anti-wrinkle, tender skin, hair care;
(2), reparation property: nanometre collagen oligopeptide has unique repairing effect, and is good with the affinity of surrounding tissue, has the effect of outstanding repair tissue, for scar, pachylosis unique repairing effect arranged, and increases the smoothness and the elasticity of skin fast;
(3), moisture retention: owing to contain a large amount of hydrophilic groups in the nanometre collagen oligopeptide, make it to have had the good moisture preserving effect, can reach the purpose that keeps the moist degree of skin.It can go deep into deep skin, reaches deep moisturizing, long-acting effect of preserving moisture;
(4), water absorbability: nanometre collagen oligopeptide has extremely strong hygroscopic property, and the exsiccant powder is exposed in the air, can absorb airborne moisture rapidly.
(5), affinity: skin has good sorption to nanometre collagen oligopeptide.The nanometre collagen oligopeptide molecular weight is little, and the peptide chain of its different peptide sections does not have mutual hinge, but is linear structure.Experiment shows that the skin absorption rate of nanometre collagen oligopeptide almost reaches 100%, and the collagen protein specific absorption is 0%～2.5%, the specific absorption about 40% of collagen peptide.
(6), good solubility: nanometre collagen oligopeptide contains a large amount of hydrophilic radicals (COOH ,-NH
2,-OH), thus make the interaction between nanometre collagen oligopeptide and the water molecules strengthen its water-soluble greatly increasing.
(7), reduced immunogenicity, safety have no side effect: nanometre collagen oligopeptide, itself be exactly protein substance, without any side effects, can eat for a long time, application.
The main application fields of the nanometre collagen oligopeptide that obtains with the inventive method is:
Makeup: nanometre collagen oligopeptide preserves moisture in the pure natural brute force, rapidly smoothing wrinkle, fast repair scar, level and smooth skin, tender skin health care, whitening and speckle dispelling, moisten aspects such as healthy hair, chest enlarge, have irreplaceable outstanding advantage, can in beauty treatment, skin makeup, hairdressing makeup, be extensive use of.
Biological, application medically: plastic surgeries such as blood vessel reparation, soluble collagen, wound burn repair materials, injection, be used for tetter such as rhagadia of the hand and foot, skin itching, ichthyosis, internal medicine diseases such as chronic gastritis, duodenal ulcer, stomach ulcer, rheumatoid arthritis, osteoporosis, liver cirrhosis, lupus erythematosus, broken skin disease, ox-hide moss, vitiligo, cell junction obstacle, capillary fragility increase, the difficult healing of wound, dental haemorrhage swelling are caused collagenosiss such as vitamin C deficiency, all be widely used.
Also there is purposes very widely in field such as cosmetic health product, nutritive food.
The present invention is further described below in conjunction with embodiment:
Embodiment 1: after the fresh pig skin depilation, scrape fat, clean, pay and be broken into fritter, earlier with the saturated limewater immersion of 10 times of volumes 40 days, clean with water rinse, be washed near neutral, use the HCl of the 1M of 10 times of volumes again, soaked 48 hours, and be washed to pH and equal about 4, obtain the pre-treatment pigskin.Get pre-treatment pigskin 200g, put into the reaction flask of Rotary Evaporators, add water 1800 grams, add 2g stomach en-and 2g trypsinase respectively, keep vacuum tightness 1～10KPa, 50 ℃ of constant temperature hydrolysis 4 hours, add papoid 2 grams then, NaOH adjust pH with 0.1M is 5, under the same vacuum condition, and 60 ℃ of constant temperature hydrolysis 3 hours.Add matrix metalloproteinase 2 grams then, continue 60 ℃ of constant temperature, hydrolysis is 3 hours under the same vacuum condition.Be heated to 100 ℃, 10 minutes, be cooled to 60 ℃ after, absorbent cotton filters, activated carbon filtration then, product is a golden transparent liquid.By spraying drying, obtain the white powder nanometre collagen oligopeptide then.
Adopt the 835-50 of Hitachi amino acidanalyser assay products amino acid to form.As shown in table 1;
Table 1 aminoacids content Content of amino acid
| Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) | Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) |
| Asp (asparagus fern) | 5.423 | Ile (different bright) | 1.035 |
| Thr (Soviet Union) | 1.623 | Ieu (bright) | 0.000 |
| Ser (silk) | 3.266 | Tyr (junket) | 2.530 |
| Glu (paddy) | 9.447 | Phe (phenylpropyl alcohol) | 1.025 |
| Gly (sweet) | 24.253 | Lys (relying) | 1.452 |
| Ala (third) | 9.287 | His (group) | 0.630 |
| Cys (Guang) | 0.085 | Arg (essence) | 8.102 |
| Val (figured silk fabrics) | 2.220 | Pro (dried meat) | 13.781 |
| Met (egg) | 0.843 | Hypro (hydroxyl dried meat) | 10.981 |
| TOTAL (total content) | 100.1 | ||
Table 1 amino acid analysis is the result show, hydrolysate has the feature amino acid of collagen protein and forms, product collagen feature amino acid consists of glycine (glycine) 24%, proline (proline) 14% and hydroxyl proline (hdroxyproline) 11%, L-glutamic acid 9%, arginine 8%.
Fig. 1 is the molecular weight distribution synoptic diagram that electron spray mass spectrometry (ESI/MS) detects the product nanometre collagen oligopeptide.The ESI/MS analytical results shows, molecular weight of product 90% is distributed between 100～1000D, be the small molecules combination of dipeptides to seven peptides, the Gaussian distribution peak molecular weight is about 500D, be mainly tripeptides, tetrapeptide, pentapeptide, so product is collagen oligopeptide, its molecular size and length should be between several nanometers to tens nanometers, be nanoscale molecular, so hydrolysate is a nanometre collagen oligopeptide.Embodiment 2: after the fresh pig skin depilation, scrape fat, clean, pay and be broken into fritter, earlier with the saturated limewater immersion of 10 times of volumes 40 days, clean with water rinse, be washed near neutral, use the HCl of the 1M of 10 times of volumes again, soaked 48 hours, and be washed to pH and equal about 4, obtain the pre-treatment pigskin.Get pre-treatment pigskin 200g, put into the reaction flask of Rotary Evaporators, add water 1800 grams, add 2g stomach en-and 2g trypsinase respectively, keep vacuum tightness 10～30KPa, 50 ℃ of constant temperature hydrolysis 4 hours, add bromeline 2 grams then, NaOH adjust pH with 0.1M is 5, under the same vacuum condition, and 72 ℃ of constant temperature hydrolysis 3 hours.Add matrix metalloproteinase 2 grams then, continue 60 ℃ of constant temperature, hydrolysis is 3 hours under the same vacuum condition.Be heated to 100 ℃, 10 minutes, be cooled to 60 ℃ after, absorbent cotton filters, activated carbon filtration then, product is a golden transparent liquid.By spraying drying, obtain the white powder nanometre collagen oligopeptide then.Amino acidanalyser assay products amino acid is formed.As shown in table 2;
Table 2 aminoacids content Content of amino acid
| Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) | Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) |
| Asp (asparagus fern) | 5.123 | Ile (different bright) | 1.015 |
| Thr (Soviet Union) | 1.626 | Ieu (bright) | 0.000 |
| Ser (silk) | 3.263 | Tyr (junket) | 2.551 |
| Glu (paddy) | 9.447 | Phe (phenylpropyl alcohol) | 1.028 |
| Gly (sweet) | 24.554 | Lys (relying) | 1.450 |
| Ala (third) | 9.286 | His (group) | 0.625 |
| Cys (Guang) | 0.087 | Arg (essence) | 8.112 |
| Val (figured silk fabrics) | 2.222 | Pro (dried meat) | 13.760 |
| Met (egg) | 0.840 | Hypro (hydroxyl dried meat) | 11.005 |
| TOTAL (total content) | 100.3 | ||
Embodiment 3: after the fresh pig skin depilation, scrape fat, clean, pay and be broken into fritter, earlier with the saturated limewater immersion of 10 times of volumes 40 days, clean with water rinse, be washed near neutral, use the HCl of the 1M of 10 times of volumes again, soaked 48 hours, water Shen equals about 4 to pH, obtains the pre-treatment pigskin.Get pre-treatment pigskin 200g, put into the reaction flask of Rotary Evaporators, add water 1800 grams, add 2g stomach en-and 2g trypsinase respectively, keep vacuum tightness 10～30KPa, 40 ℃ of constant temperature hydrolysis 4 hours, add papoid 2 grams then, with the NaOH of the 0.1M pH value of withering is 5, under the same vacuum condition, and 70 ℃ of constant temperature hydrolysis 3 hours.Add matrix metalloproteinase 2 grams then, continue 60 ℃ of constant temperature, hydrolysis is 3 hours under the same vacuum condition.Be heated to 100 ℃, 10 minutes, be cooled to 60 ℃ after, absorbent cotton filters, activated carbon filtration then, product is a golden transparent liquid.By spraying drying, obtain the white powder nanometre collagen oligopeptide then.The acid of product ammonia highland is formed as shown in table 3:
Embodiment 4: after the fresh pig skin depilation, scrape fat, clean, pay and be broken into fritter, the saturated limewater with l0 times of volume soaked 40 days earlier, clean with water rinse, be washed near neutral, use the HCl of the 1M of 10 times of volumes again, soaked 48 hours, and be washed to pH and equal about 4, obtain the pre-treatment pigskin.Get pre-treatment pigskin 600g, put into the reaction flask of Rotary Evaporators, add water 1800 grams, add 4g stomach en-and 4g trypsinase respectively, keep vacuum tightness 10～30KPa, 50 ℃ of constant temperature hydrolysis 4 are little
Table 3 aminoacids content Content of amino acid
| Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) | Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) |
| Asp (asparagus fern) | 5.221 | Ile (different bright) | 1.145 |
| Thr (Soviet Union) | 1.219 | Ieu (bright) | 0.000 |
| Ser (silk) | 3.567 | Tyr (junket) | 2.641 |
| Glu (paddy) | 9.332 | Phe (phenylpropyl alcohol) | 1.122 |
| Gly (sweet) | 24.420 | Lys (relying) | 1.321 |
| Ala (third) | 9.161 | His (group) | 0.520 |
| Cys (Guang) | 0.070 | Arg (essence) | 8.006 |
| Val (figured silk fabrics) | 2.115 | Pro (dried meat) | 13.562 |
| Met (egg) | 0.751 | Hypro (hydroxyl dried meat) | 10.635 |
| TOTAL (total content) | 99.6 | ||
The time, add papoid 4 gram then, be 5 with the NaOH adjust pH of 0.1M, equally under the vacuum condition, 60 ℃ of constant temperature hydrolysis 3 hours.Add matrix metalloproteinase 4 grams then, continue 60 ℃ of constant temperature, hydrolysis is 3 hours under the same vacuum condition.Be heated to 100 ℃, 10 minutes, be cooled to 60 ℃ after, absorbent cotton filters, activated carbon filtration then, product is a golden transparent liquid.By spraying drying, obtain the white powder nanometre collagen oligopeptide then.Product amino acid is formed as shown in table 4; Embodiment 5: after the fresh pig skin depilation, scrape fat, clean, pay and be broken into fritter, earlier with the saturated limewater immersion of 10 times of volumes 40 days, clean with water rinse, be washed near neutral, use the HCl of the 1M of 10 times of volumes again, soaked 48 hours, and be washed to pH and equal about 4, obtain the pre-treatment pigskin.Get pre-treatment pigskin 200g, put into the reaction flask of Rotary Evaporators, add water 1800 grams, add 2g stomach en-and 2g trypsinase respectively, keep vacuum tightness 10～30KPa, 20 ℃ of constant temperature hydrolysis 8 hours, add papoid 2 grams then, NaOH adjust pH with 0.1M is 5, under the same vacuum condition, and 75 ℃ of constant temperature hydrolysis 3 hours.Add matrix metalloproteinase 2 grams then, continue 60 ℃ of constant temperature, hydrolysis is 3 hours under the same vacuum condition.Be heated to 100 ℃, 10 minutes, be cooled to 60 ℃ after, absorbent cotton filters, activated carbon filtration then, product is a golden transparent liquid.By spraying drying, obtain the white powder nanometre collagen oligopeptide then.Product amino acid is formed as shown in table 5;
Table 4 aminoacids content Content of amino acid
| Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) | Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) |
| Asp (asparagus fern) | 5.125 | Ile (different bright) | 1.141 |
| Thr (Soviet Union) | 1.626 | Ieu (bright) | 0.000 |
| Ser (silk) | 3.322 | Tyr (junket) | 2.629 |
| Glu (paddy) | 9.526 | Phe (phenylpropyl alcohol) | 1.117 |
| Gly (sweet) | 24.461 | Lys (relying) | 1.592 |
| Ala (third) | 9.288 | His (group) | 0.631 |
| Cys (Guang) | 0.072 | Arg (essence) | 8.115 |
| Val (figured silk fabrics) | 2.320 | Pro (dried meat) | 13.883 |
| Met (egg) | 0.843 | Hypro (hydroxyl dried meat) | 10.872 |
| TOTAL (total content) | 100.5 | ||
Table 5 aminoacids content Content of amino acid
| Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) | Amino acid title Amino Acid | Detected result Analysis Result (mg/100mg) |
| Asp (asparagus fern) | 5.326 | Ile (different bright) | 1.045 |
| Thr (Soviet Union) | 1.598 | Ieu (bright) | 0.000 |
| Ser (silk) | 3.099 | Tyr (junket) | 2.319 |
| Glu (paddy) | 9.315 | Phe (phenylpropyl alcohol) | 1.201 |
| Gly (sweet) | 24.341 | Lys (relying) | 1.381 |
| Ala (third) | 9.380 | His (group) | 0.521 |
| Cys (Guang) | 0.096 | Arg (essence) | 8.007 |
| Val (figured silk fabrics) | 2.331 | Pro (dried meat) | 13.692 |
| Met (egg) | 0.810 | Hypro (hydroxyl dried meat) | 10.762 |
| TOTAL (total content) | 99.2 | ||
Claims (10)
1, a kind of nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin is characterized in that molecular weight mainly is distributed between 100～1000D, is the small molecules combination of dipeptides to seven peptides, and the Gaussian distribution peak molecular weight is about 500D.
2, according to the described nanometre collagen oligopeptide that the hydrolysis extraction obtains from animal skin of claim 1, be mainly tripeptides, tetrapeptide, pentapeptide, its molecular size and length are between several nanometers to tens nanometer.
3, the preparation method of claim 1 or the 2 described nanometre collagen oligopeptides that the hydrolysis extraction obtains from animal skin, comprise that the animal skin that will contain collagen protein is through pre-treatment, adopt the combined-enzyme method hydrolysis to extract collagen protein, macromolecular collagen protein is hydrolyzed into micromolecular collagen oligopeptide.
4, according to claim 3, it is characterized in that, preprocessing process is after aquatic foods are raiseeed skin depilation, scrapes fat, cleans, pays and be broken into fritter, earlier with the saturated limewater immersion of 10 times of volumes 40 days, clean with water rinse, be washed near neutral, use the HCl of the 1M of 10 times of volumes again, soaked 48 hours, be washed to pH near about 4, obtain the pre-treatment animal skin.
5,, it is characterized in that the enzyme that hydrolysis is adopted is meant combinations several in trypsinase, stomach en-, papoid, Sumizyme MP, matrix metalloproteinase, neutral protease, the bromeline according to claim 3.
6, according to claim 3, it is characterized in that the consumption of every kind of enzyme is 0.5～2% of poultry tare weight amount, the hydrolysis pH of every kind of enzyme is 3～10, hydrolysis time is 1～8 hour.
7,, it is characterized in that the temperature of extracting nanometre collagen oligopeptide is 20-75 ℃ according to claim 3.
8,, it is characterized in that the hydrolysis reaction that extracts nanometre collagen oligopeptide carries out under vacuum condition according to claim 3.
9,, it is characterized in that the hydrolysed mix solid content that extracts the nanometre collagen oligopeptide employing is 1-40% according to claim 3.
10, according to each the nanometre collagen oligopeptide of claim 1-9 in the application of medicine, healthcare products, makeup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100986529A CN1966516B (en) | 2006-07-11 | 2006-07-11 | Nanometre collagen oligopeptide and its producing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100986529A CN1966516B (en) | 2006-07-11 | 2006-07-11 | Nanometre collagen oligopeptide and its producing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1966516A true CN1966516A (en) | 2007-05-23 |
| CN1966516B CN1966516B (en) | 2013-04-17 |
Family
ID=38075522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006100986529A Active CN1966516B (en) | 2006-07-11 | 2006-07-11 | Nanometre collagen oligopeptide and its producing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1966516B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011060583A1 (en) * | 2009-11-19 | 2011-05-26 | 浙江大学 | Nonnatural collagen-like protein and use thereof |
| CN101787078B (en) * | 2009-01-23 | 2013-01-09 | 香港百特有限公司 | Collagen polypeptide, preparation method and application thereof |
| CN104232715A (en) * | 2014-08-20 | 2014-12-24 | 青岛贝尔特生物科技有限公司 | Preparation method of protein oligopeptide |
| CN104324367A (en) * | 2014-10-20 | 2015-02-04 | 广西壮族自治区花红药业股份有限公司 | Novel application of fish scale extract in preparation of medicine for treating and/or preventing leukoderma |
| CN104324054A (en) * | 2014-10-20 | 2015-02-04 | 广西壮族自治区花红药业股份有限公司 | Novel application of I-type collagen in preparation of medicine for treating and/or preventing leukoderma |
| CN108371255A (en) * | 2018-02-08 | 2018-08-07 | 青岛彬利宠物用品有限公司 | A kind of dog food cat food and preparation method thereof preparing OCO collagen small peptides using enzymolysis process |
-
2006
- 2006-07-11 CN CN2006100986529A patent/CN1966516B/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787078B (en) * | 2009-01-23 | 2013-01-09 | 香港百特有限公司 | Collagen polypeptide, preparation method and application thereof |
| WO2011060583A1 (en) * | 2009-11-19 | 2011-05-26 | 浙江大学 | Nonnatural collagen-like protein and use thereof |
| CN102164949B (en) * | 2009-11-19 | 2013-10-23 | 浙江大学 | Nonnatural protein |
| CN104232715A (en) * | 2014-08-20 | 2014-12-24 | 青岛贝尔特生物科技有限公司 | Preparation method of protein oligopeptide |
| CN104324367A (en) * | 2014-10-20 | 2015-02-04 | 广西壮族自治区花红药业股份有限公司 | Novel application of fish scale extract in preparation of medicine for treating and/or preventing leukoderma |
| CN104324054A (en) * | 2014-10-20 | 2015-02-04 | 广西壮族自治区花红药业股份有限公司 | Novel application of I-type collagen in preparation of medicine for treating and/or preventing leukoderma |
| CN108371255A (en) * | 2018-02-08 | 2018-08-07 | 青岛彬利宠物用品有限公司 | A kind of dog food cat food and preparation method thereof preparing OCO collagen small peptides using enzymolysis process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1966516B (en) | 2013-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101289507B (en) | Collagen protein and collagen polypeptides, preparation thereof and applications | |
| CN106344427A (en) | Polypeptide composition for facial care and preparation method of polypeptide composition | |
| US20070110693A1 (en) | Compositions containing peptide copper complexes and soft tissue fillers, and methods related thereto | |
| CN1966516B (en) | Nanometre collagen oligopeptide and its producing method | |
| CN101693108A (en) | Oral nutrition composition for beautifying skin | |
| CN1896268A (en) | Extraction of activated micromolecular sheept germine from sheep embryo and its use | |
| KR20090014150A (en) | Synthetic peptides used for treating the skin and use thereof in cosmetic or dermopharmaceutical compositions | |
| CN107496310A (en) | A kind of facial treatment essence liquid and preparation method thereof | |
| CN104000744A (en) | Hairdressing liquid capable of improving metabolism of skin and delaying senescence and production method of hairdressing liquid capable of improving metabolism of skin and delaying senescence | |
| CN101721343A (en) | Deep-sea fish skin collagen peptide firming and anti-ageing face mask and preparing method | |
| CN107929091A (en) | A kind of extracting method of high activity collagen and its application | |
| CN105769725A (en) | Rosemary moisturizing water and making method thereof | |
| KR101697152B1 (en) | Manufacturing method of cosmetic material having an anti-aging effect using mucin from eel skin | |
| KR101339423B1 (en) | cosmetic composition having collagen derived from fishes and manufacturing method thereof | |
| JP3802721B2 (en) | Biological collagen synthesis promoter | |
| CN110368347A (en) | Anti-ageing reparation double-layer bi-color essence of one kind and preparation method thereof | |
| CN113425829A (en) | Cosmetic or pharmaceutical product comprising active polypeptide and process for preparing same | |
| CN102247589A (en) | Application of marine collagen peptide in preparing medicament and food for delaying skin aging | |
| CN109730943A (en) | A kind of anti-wrinkle composition | |
| CN102150866A (en) | Cystine-containing complex and preparation method thereof | |
| CN106420392A (en) | Collagen gel with congelation state | |
| CN107496194A (en) | A kind of surfactant and preparation method thereof | |
| CN111961119B (en) | Application of polypeptide in preparation of medicine or cosmetic for promoting collagen secretion | |
| CN108653143A (en) | A kind of facial treatment essence liquid | |
| CN1850031A (en) | Embossed-rod Chinese medicine moisture-keeping mask powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhong Wanghong Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Du Yibo Document name: payment instructions |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Du Yibo Document name: payment instructions |
|
| DD01 | Delivery of document by public notice |