CN106344427A - Polypeptide composition for facial care and preparation method of polypeptide composition - Google Patents
Polypeptide composition for facial care and preparation method of polypeptide composition Download PDFInfo
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- CN106344427A CN106344427A CN201610894183.5A CN201610894183A CN106344427A CN 106344427 A CN106344427 A CN 106344427A CN 201610894183 A CN201610894183 A CN 201610894183A CN 106344427 A CN106344427 A CN 106344427A
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- polypeptide complex
- oligopeptide
- mixed liquor
- polypeptide
- care
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention discloses a polypeptide composition for facial care. The polypeptide composition comprises the following components in percentage by mass: 0.0001-0.0005% of acetyl hexapeptide-8, 0.01-0.1% of acetyl tetrapeptide-5, 0.02-0.1% of tripeptide-1 copper, 0.0005-0.1% of glutathione, 0.0001-0.0005% of oligopeptide-1, 0.0001-0.0005% of oligopeptide-2, 0.0001-0.0005% of oligopeptide-3, 0.0001-0.0005% of oligopeptide-4, 0.0001-0.0005% of oligopeptide-5, 1.0-5.0% of polyethylene glycol, 84.697-97.9689% of mannitol and 1.0-10.0% of deionized water. The polypeptide composition disclosed by the invention comprises 9 polypeptide active matters, has the advantages of remedying and whitening skin and preventing aging, and is capable of permeating into skin for improving blood circulation of the face, supplying sufficient nutrients and energy to cells and promoting metabolism of the skin, and thus the purposes of remedying and caring skin can be achieved; and a freeze-drying technique is adopted in a production process, so that various active substances can be retained to the maximum extent, original properties of various active substances can be maintained, conversion of active matters can be prevented, and the stability of a product can be improved.
Description
Technical field
The present invention relates to cosmetic technical field is and in particular to a kind of polypeptide complex for facial-care and its preparation
Method, this polypeptide complex is exclusively used in the daily nursings such as facial reparation, anti-ageing, whitening.
Background technology
Polypeptide is the compound that a-amino acid is formed so that peptide bond links together, and it is also the middle product of proteolysis
Thing.Dipeptides is called by the compound of two amino acid molecular dehydrating condensations, analogizes also tripeptides, tetrapeptide, pentapeptide etc. in the same manner.
Generally can be become by the compound of three or more than three amino acid molecular dehydrating condensations and be polypeptide.
In nature, most chemical reactions, biological respinse and evolutionary process are by specific aminoacid sequence to a certain extent
Row regulation and control.Based on this, effect that the peptide of various different structures has is also each different.These interactions can be transcribed
Peptide sequence and there is induction produce the biologic activity of peptide that is small fragment, activity stabilized and can synthesizing and started
Dermatology and the new molecular application of skin care applications.Such as, infection, pigmentation, cell proliferation and differentiation, vascularization,
Heredoimmunity and extracellular matrix synthesis etc..
At present there are various cosmetics or skin care item on the market, but most of skin care item only add a certain class effect
Polypeptide as skin care item effect active matter, the effect that therefore skin obtains is more single, and slowly effect；Furthermore, current market
On major part with polypeptide for the skin care item of active effect thing, be all that polypeptide or polypeptide stock solution are directly added on conventional skin care
In product substrate, consider, the addition of polypeptide can be limited to based on to the making of whole formula and stability, therefore, consumer
The polypeptide containing in the product being intended for single use is very few, has little effect.
Present invention effect survey of demands result to everyday skincare based on market comsupton crowd, through multiple orthogonal experiment
After screening, it is finally that main active matter is prepared for a polypeptide complex for facial total care with 9 kinds of polypeptides, to meet
Reach desired by consumers in general integrates reparation, whitening, the face protection product of anti-ageing several large effects；Additionally, for understanding
The difficult problem that certainly in generally conventional skin care item, active matter addition is limited to, the polypeptide complex in the present invention is adopted in production technology
With freeze drying technology, apply this Technology to make the polypeptide complex of the present invention, product can be made to reach skin total care work(
Effect is strong, active matter is difficult to lose efficacy, product is stable, the advantages of easily preserve.
Content of the invention
It is an object of the invention to provide a kind of polypeptide complex for facial-care, this polypeptide complex contains 9 kinds
Polypeptide active thing, it has the advantages that to integrate reparation, whitening and anti-ageing, can penetrate into skin, improves facial blood circulation,
The enough nutrients of supply cell and energy, promote the metabolism of skin, thus reaching the purpose repaired with skin care.With
When, present invention also offers the preparation method of this polypeptide complex, this polypeptide complex employs lyophilizing skill in production technology
Art, can avoid the conversion of active matter and the stability improving product.
The technical solution used in the present invention is: a kind of polypeptide complex for facial-care, by mass percentage,
Including following components:
Argireline 0.0001%-0.0005%,
Acetyl group tetrapeptide -5 0.01%-0.1%,
Tripeptides -1 copper 0.02%-0.1%,
Glutathione 0.0005%-0.1%,
Oligopeptide -1 0.0001%-0.0005%,
Oligopeptide -2 0.0001%-0.0005%,
Oligopeptide -3 0.0001%-0.0005%,
Oligopeptide -4 0.0001%-0.0005%,
Oligopeptide -5 0.0001%-0.0005%,
Polyethylene Glycol 1.0%-5.0%,
Mannitol 84.697%-97.9689%
Deionized water 1.0%-10.0%.
The polypeptide complex for facial-care of the present invention contains 9 kinds of polypeptide active things, effect of each polypeptide active thing
As follows:
(1) oligopeptide -1: also known as epidermal growth factor, the proliferation and differentiation of epidermis cell can be promoted, thus replacing with regenerative cell
Senile cell and the process of dead cell, quickly repair damaged skin, improve reparation, the nursing of skin；Also have and calm down eye
Wrinkle, firm skin, skin care, strengthen skin elasticity；Mitigate the injury to skin for the ultraviolet radiation；Anti-acne and remove cicatrix
Effect.
(2) oligopeptide -2: also known as insulin like growth factor, be mitotic polypeptide growth factor, it can promote flesh
The propagation growth of the various kinds of cell such as meat cell, osteocyte and chondrocytes in vitro tissue, therefore has good repairing in the treatment of skin
Multiple, anti-ageing effect.
(3) oligopeptide -3: also known as basic fibroblast growth factor, it is adjusting cell survival, cell division, blood vessel shape
Play an important role in one-tenth, cell differentiation and cell migration, to reduce and to prevent by promoting epidermal growth and migration
The generation of wrinkle, can promote fibroblast to divide, thus promoting the formation of collagen protein and elastin laminin, mitigate skin pigment
Calmness, strengthen skin elasticity.
(4) oligopeptide -4: be a kind of active collagen precursor, it can help new collagen fabric formation (solvable
Property collagen protein), skin exists more soluble collagens, the elasticity of skin will be better.
(5) oligopeptide -5: also known as keratinocyte growth factor, growth, differentiation and the migration of epithelial cell can be promoted, there is pole
Strong wound healing function, promotes skin repair, prevents moisture loss, promotes the metabolic balance of skin histology, strengthens skin
Skin elasticity.
(6) Glutathione: be by glutamic acid, cysteine and glycine combine, containing sulfydryl tripeptides, there is antioxygen
Change effect and integration Detoxication.Glutathione is a kind of peptide of synthesis naturally in human cell's matter, can effectively delay thin
The aging of born of the same parents, accelerates the regeneration of cell, at the same time it can also by affecting Skin Cell tyrosinase activity, suppression melanin life
Become, prevent the generation of skin splash, therefore, it can give the outstanding whitening of skin and anti-ageing function.
(7) Argireline: be a kind of neurotransmitter suppression class victory peptide, its antiwrinkle active is high, Small side effects can be short
Mitigate in phase and shrink, by histrionic muscless, the wrinkle causing, especially near the eyes, the wrinkle of forehead and the corners of the mouth, it can reduce existing face
Portion's wrinkle, can effectively prevent new wrinkle from producing again.
(8) acetyl group tetrapeptide -5: be a kind of inhibitor of angiotensin converting enzyme, eye lymph and capillary can be improved
The microcirculation of blood vessel, eliminates edema, mitigates black eye, improves skin elasticity and smoothness；Meanwhile, it also has suppression collagen protein
Glycosylated effect, can prevent that collagen protein is aging, assemble, thus further up to mitigating eye pouch and crease-resistant effect.
(9) tripeptides -1 copper: copper ion and tripeptides have preferable affinity, can spontaneously form complex, thus be conducive to by
Absorption of human body, wherein copper ion have good repair for impaired skin, and the skin that simultaneously compacts improves elasticity, polypeptide moiety
The synthesis of collagen protein can be promoted, smooth microgroove and wrinkle.
Preferably, the water content of the described polypeptide complex for facial-care is less than 5%.Water content is little, is conducive to producing
The preservation of product and the stability keeping product.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor；
2) filtration sterilization: using microporous filter membrane by polypeptide complex mixed liquor filtration sterilization, obtain aseptic polypeptide complex and mix
Close liquid；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put in vacuum freeze drier, carries out lyophilization, obtain
The polypeptide complex being dried；
5) tamponade: cillin bottle is compressed by the plug using sterilizing；Will fill polypeptide complex mixed liquor, after lyophilization half
The cillin bottle compression of tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) is processed, you can obtain the polypeptide for facial-care
Complex.
Preferably, the mixing speed in step 1) is 30-60 r/min, and the time of stirring is 0.5-1h, and stirring is mainly
Material is made to be mixed thoroughly, the time can be adjusted according to specific mixing situation, and process probably needs 0.5-1h really.
Preferably, step 2) in adopt microporous filter membrane aperture be 0.1-0.5um.
Preferably, after adopting the aseptic polypeptide complex mixed liquor of cillin bottle fill in step 3), using the plug of sterilizing
By cillin bottle half tamponade.Half tamponade can prevent medicine to run off and foreign body enters, and air can be kept relatively to circulate again.
Preferably, the polypeptide complex mixed liquor of fill is cooled to -40 ± 2 DEG C by the freezing dry process in step 4),
The cryodesiccated time is 24-72h.
Preferably, in step 6), gland encapsulation is carried out using the dry aluminium lid of cleaning.
This polypeptide complex employs Freeze Drying Technique in production technology, and Freeze Drying Technique has lot of advantages,
It makes the moisture content in the material freezing in advance directly from Bing Taisheng Huawei steam using sublimation principle mainly under vacuum conditions
And being removed, so that dry materials.Vacuum lyophilization is widely used in multiple product it can be ensured that protein, dimension in article
The multiple nutritional components such as raw element, particularly those volatile temperature-sensitive sexual element are not lost, thus can keep original to greatest extent
Nutritional labeling moreover it is possible to effectively prevent the oxidation in dry run, conversion and the state change of nutritional labeling can be avoided.Lyophilizing
Product afterwards is in spongy, no drying shrinkage, rehydration be good, moisture content is few, can preserve for a long time at normal temperatures after product packaging and
Transport.Therefore, the polypeptide complex of the present invention is dried process using lyophilization, can farthest remain polypeptide multiple
The various active substances of compound, keep the original performance of various active substances, play maximum effect.
Cillin bottle fill polypeptide complex is preferably used, used in it, cillin bottle and plug are intended to adopt in process of the present invention
Deionized water cleans up, and carries out sterilization treatment, in case various living contaminantses product, thus affecting the performance of product.
The invention has the beneficial effects as follows: the polypeptide complex of the present invention contains 9 kinds of polypeptide active things, its have collection repair,
Whitening and the anti-ageing advantage in one, can penetrate into skin, improve facial blood circulation, the enough nutrients of supply cell and energy
Amount, promotes the metabolism of skin, thus reaching the purpose repaired with skin care.The polypeptide complex of the present invention is producing work
Employ Freeze Drying Technique in skill, can farthest remain various active substances, keep various active substances original
Performance, can avoid the conversion of active matter and the stability improving product.
Brief description
Fig. 1 is the preparation flow figure of the polypeptide complex for facial-care.
Specific embodiment
Embodiment 1
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.0001% acetyl group six
Peptide -8,0.01% acetyl group tetrapeptide -5,0.02% tripeptides -1 copper, 0.0005% Glutathione, 0.0001% oligopeptide -1,0.0001% is few
Peptide -2,0.0001% oligopeptide -3,0.0001% oligopeptide -4,0.0001% oligopeptide -5,1.0% Polyethylene Glycol, 88.9689% mannose
Alcohol, 10% deionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein stir
Mixing speed is 30 r/min, and the time of stirring is 1h；
2) filtration sterilization: using the microporous filter membrane for 0.1um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 32h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Embodiment 2
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.0002% acetyl group six
Peptide -8,0.1% acetyl group tetrapeptide -5,0.05% tripeptides -1 copper, 0.1% Glutathione, 0.0002% oligopeptide -1,0.0002% oligopeptide -2,
0.0002% oligopeptide -3,0.0002% oligopeptide -4,0.0002% oligopeptide -5,2.0% Polyethylene Glycol, 89.7488% mannitol, 8% goes
Ionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein stir
Mixing speed is 60r/min, and the time of stirring is 0.6h；
2) filtration sterilization: using the microporous filter membrane for 0.2um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 35h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Embodiment 3
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.0003% acetyl group six
Peptide -8,0.1% acetyl group tetrapeptide -5,0.08% tripeptides -1 copper, 0.05% Glutathione, 0.0003% oligopeptide -1,0.0003% oligopeptide -
2,0.0003% oligopeptide -3,0.0003% oligopeptide -4,0.0003% oligopeptide -5,3.0% Polyethylene Glycol, 90.7682% mannitol, 6%
Deionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein stir
Mixing speed is 50 r/min, and the time of stirring is 0.8h；
2) filtration sterilization: using the microporous filter membrane for 0.5um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 40h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Embodiment 4
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.0005% acetyl group six
Peptide -8,0.1% acetyl group tetrapeptide -5,0.1% tripeptides -1 copper, 0.1% Glutathione, 0.0005% oligopeptide -1,0.0005% oligopeptide -2,
0.0005% oligopeptide -3,0.0005% oligopeptide -4,0.0005% oligopeptide -5,5.0% Polyethylene Glycol, 89.697% mannitol, 5% goes
Ionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein stir
Mixing speed is 50 r/min, and the time of stirring is 0.8h；
2) filtration sterilization: using the microporous filter membrane for 0.3um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 45h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Comparative example 1
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.0002% acetyl group six
Peptide -8,0.02% acetyl group tetrapeptide -5,0.05% tripeptides -1 copper, 0.05% Glutathione, 3% Polyethylene Glycol, 86.8798% mannose
Alcohol, 10% deionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione again, continue to stir
Mix to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein mixing speed is 40 r/min, the time of stirring is 1h；
2) filtration sterilization: using the microporous filter membrane for 0.4um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 42h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Comparative example 2
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.02% acetyl group tetrapeptide-
5,0.05% tripeptides -1 copper, 0.03% Glutathione, 0.0002% oligopeptide -1,0.0001% oligopeptide -2,0.0002% oligopeptide -3,
0.0004% oligopeptide -4,0.0002% oligopeptide -5,3% Polyethylene Glycol, 88.8989% mannitol, 8% deionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein stir
Mixing speed is 40r/min, and the time of stirring is 0.8h；
2) filtration sterilization: using the microporous filter membrane for 0.5um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 40h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Comparative example 3
A kind of polypeptide complex for facial-care, by mass percentage, including following components: 0.0003% acetyl group six
Peptide -8,0.05% tripeptides -1 copper, 0.0006% Glutathione, 0.0003% oligopeptide -1,0.0003% oligopeptide -2,0.0002% oligopeptide -3,
0.0003% oligopeptide -4,0.0002% oligopeptide -5,2% Polyethylene Glycol, 87.9478% mannitol, 10% deionized water.
The method preparing this polypeptide complex for facial-care, comprises the following steps:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor, wherein stir
Mixing speed is 30 r/min, and the time of stirring is 1h；
2) filtration sterilization: using the microporous filter membrane for 0.5um for the aperture by polypeptide complex mixed liquor filtration sterilization, obtain aseptic
Polypeptide complex mixed liquor；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put into and is cooled to -40 ± 2 DEG C in vacuum freeze drier, enter
Row lyophilization 72h, obtains the polypeptide complex of drying；
5) tamponade: using sterilizing plug by cillin bottle tamponade；
6) gland encapsulation: the polypeptide complex gland encapsulation after step 5) being processed using cleaning dry aluminium lid, you can obtain
Polypeptide complex for facial-care.
Select 245 healthy volunteers, be divided into 7 groups, the mean age is 45 years old, is respectively adopted embodiment 1-4 and contrast
Product prepared by example 1-3 is tested, daily sooner or later in face using prepared polypeptide complex, respectively using 30 days and
After 90 days, shown in the following Tables 1 and 2 of effect.
Polypeptide complex prepared by table 1 embodiment 1-4 and comparative example 1-3 is using the effect after 30 days
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Skin smoothness | Improve 33.5% | Improve 34.1% | Improve 34.6% | Improve 35.2% | Improve 29.4% | Improve 27.1% | Improve 28.2% |
| Skin-whitening degree | Improve 18.8% | Improve 20.1% | Improve 21.0% | Improve 22.3% | Improve 12.4% | Improve 15.6% | Improve 14.1% |
| Skin speckle dispelling degree | Improve 13.9% | Improve 14.6% | Improve 15.7% | Improve 16.3% | Improve 10.1% | Improve 12.8% | Improve 12.3% |
| Wrinkle number | Reduce 30.2% | Reduce 31.6% | Reduce 32.2% | Reduce 33.8% | Reduce 27.4% | Reduce 23.1% | Reduce 25.4% |
| Wrinkle depth | Shoal 23.2% | Shoal 24.3% | Shoal 25.0% | Shoal 25.9% | Shoal 19.6% | Shoal 18.2% | Shoal 18.8% |
Polypeptide complex prepared by table 2 embodiment 1-4 and comparative example 1-3 is using using the effect after 90 days
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Skin smoothness | Improve 53.5% | Improve 53.9% | Improve 54.1% | Improve 57.6% | Improve 39.2% | Improve 36.1% | Improve 37.8% |
| Skin-whitening degree degree | Improve 38.6% | Improve 39.9% | Improve 41.3% | Improve 45.3% | Improve 22.7% | Improve 25.2% | Improve 28.1% |
| Skin speckle dispelling degree | Improve 34.6% | Improve 35.8% | Improve 36.2% | Improve 37.3% | Improve 20.4% | Improve 25.8% | Improve 24.8% |
| Wrinkle number | Reduce 40.2% | Reduce 41.2% | Reduce 42.5% | Reduce 45.8% | Reduce 27.4% | Reduce 26.1% | Reduce 29.1% |
| Wrinkle depth | Shoal 33.6% | Shoal 34.8% | Shoal 35.4% | Shoal 36.3% | Shoal 26.9% | Shoal 25.7% | Shoal 26.3% |
Claims (7)
1. a kind of polypeptide complex for facial-care it is characterised in that by mass percentage, including following components:
Argireline 0.0001%-0.0005%,
Acetyl group tetrapeptide -5 0.01%-0.1%,
Tripeptides -1 copper 0.02%-0.1%,
Glutathione 0.0005%-0.1%,
Oligopeptide -1 0.0001%-0.0005%,
Oligopeptide -2 0.0001%-0.0005%,
Oligopeptide -3 0.0001%-0.0005%,
Oligopeptide -4 0.0001%-0.0005%,
Oligopeptide -5 0.0001%-0.0005%,
Polyethylene Glycol 1.0%-5.0%,
Mannitol 84.697%-97.9689%
Deionized water 1.0%-10.0%.
2. the polypeptide complex for facial-care according to claim 1 is it is characterised in that described protects for face
The water content of the polypeptide complex of reason is less than 5%.
3. preparation claim 1 or 2 described in the polypeptide complex for facial-care method it is characterised in that include with
Lower step:
1) preparation of polypeptide complex mixed liquor: put into deionized water, mannitol and Polyethylene Glycol in agitated kettle successively, stir
Mix to feed liquid mix homogeneously, then put into again Argireline, acetyl group tetrapeptide -5, tripeptides -1 copper, Glutathione, oligopeptide -
1st, oligopeptide -2, oligopeptide -3, oligopeptide -4 and oligopeptide -5, continue stirring to feed liquid uniformly, obtain polypeptide complex mixed liquor；
2) filtration sterilization: using microporous filter membrane by polypeptide complex mixed liquor filtration sterilization, obtain aseptic polypeptide complex and mix
Close liquid；
3) fill, half tamponade: using the aseptic polypeptide complex mixed liquor of the cillin bottle fill after sterilizing-drying, blend compounds plug will
Cillin bottle half tamponade, obtains the polypeptide complex mixed liquor of fill；
4) lyophilization: the polypeptide complex mixed liquor of fill is put in vacuum freeze drier, carries out lyophilization, obtain
The polypeptide complex being dried；
5) tamponade: by the cillin bottle compression of half tamponade；
6) gland encapsulation: the polypeptide complex after step 5) is processed carries out gland encapsulation, you can obtain for facial-care
Polypeptide complex.
4. method according to claim 3, it is characterised in that the mixing speed in step 1) is 30-60 r/min, stirs
Time be 0.5-1h.
5. method according to claim 3 is it is characterised in that step 2) in the aperture of microporous filter membrane that adopts be 0.1-
0.5um.
6. method according to claim 3 is it is characterised in that the freezing dry process in step 4) is multiple by the polypeptide of fill
Compound mixed liquor is cooled to -40 ± 2 DEG C, and the cryodesiccated time is 24-72h.
7. method according to claim 3 is it is characterised in that carry out gland envelope using the dry aluminium lid of cleaning in step 6)
Dress.
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