CN104705479A - Squid active peptide extracting method - Google Patents
Squid active peptide extracting method Download PDFInfo
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- CN104705479A CN104705479A CN201510145317.9A CN201510145317A CN104705479A CN 104705479 A CN104705479 A CN 104705479A CN 201510145317 A CN201510145317 A CN 201510145317A CN 104705479 A CN104705479 A CN 104705479A
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Abstract
The invention discloses a squid active peptide extracting method. The extracting method comprises the following steps: processing raw materials, hydrolyzing, carrying out high-pressure microjet superfine grinding treatment, fermenting and implementing high-pressure treatment so as to obtain a finished product. According to the method disclosed by the invention, the content of active peptide is above 95% and the proportion of small molecule peptide is above 95%.
Description
Technical field
The present invention relates to a kind of extracting method of active peptide, be specifically related to a kind of extracting method of squid active peptide.
Background technology
Squid, also claims squid, squid, and belonging to mollusc, is the one of cuttlefish.Squid is a kind of famous and precious marine product, is important ocean siphonopods aquatic products thing, is distributed widely in each sea area in the Atlantic Ocean, the Indian Ocean and the Pacific Ocean.China squid year quantity of the catch, at about 500,000 tons, is domestic main ocean fishery kind.Also be one of kind with potentiality to be exploited most in current deep-sea fishing.Squid is rich in calcium, phosphorus, ferro element, is beneficial to skeleton development and hematopoiesis, effectively can treat anaemia; Squid also containing a large amount of taurines, can suppress the cholesterol level in blood, fatigue-relieving, recovers eyesight, improves liver function; Polypeptide contained by it and selenium have antiviral, anti-actinism.There is head, foot, liver and the epidermis the like waste accounting for squid body weight 30% to produce in squid process, and biologically active peptide can be extracted from the liver of squid, there is the effect of hypotensive, antiviral, anti-ray.
At present, from squid, obtain the method for active peptide, have acid-alkali treatment method, enzyme decomposition method, microbe fermentation method etc.As Chinese patent CN102217699B, disclose a kind of preparation method of squid skin protein active peptide, squid skin is cleaned and is cut into bulk, add NaOH aqueous solution soaking 2-8d and decolour, then through being washed to pH neutrality, add Ca (OH)
2solution soaks 2-4d and carries out fixation, again through being washed to pH neutrality, in the pH neutral water of 55-75 DEG C, process 0.5-1.5h filter, removing insoluble protein, extraction obtains fish skin protein liquor, finally, by this liquid in 110-121 DEG C of HIGH PRESSURE TREATMENT 30-120min, both obtained squid skin protein active peptide, the time of whole operation is oversize.And for example Chinese patent CN102217699B, disclose the preparation method of a kind of marine source Bacillus barbaricus SCSIO 02429CCTCC NO:M20102130 and the little tripe of a kind of squid, Bacillus barbaricusSCSIO 02429 is added have in induction product enzyme fermentation culture medium and ferment, obtain hydrolysis and use thick enzyme solutions, then add thick enzyme solutions after squid viscera being broken into pulpous state and carry out enzymolysis, obtain the crude protein zymolyte that sugar side chains is destroyed, add bromelain again and carry out enzymolysis, the zymolyte obtained is through leaving standstill, crude fat layer is separated with water layer, dry after removing fat deposit, obtain squid small peptide, the duration of whole operation is longer.The equal Shortcomings of existing method, this constrains intensive processing and the application of squid to a certain extent.
Summary of the invention
The invention provides a kind of technical maturity, be applicable to the method extracting squid active peptide from squid of production.
The present invention is achieved through the following technical solutions:
An extracting method for squid active peptide, comprises the steps:
1) Feedstock treating: rub after new Fresh squid is cleaned;
2) be hydrolyzed: the pure water adding 8-10 times amount, regulate mixture ph to 1.8-2.5, boil rear maintenance pressure 0.1Mpa and be hydrolyzed 8-10 hour, boil to maintain in press process and note supplementing the moisture lost;
3) high pressure microjet ultramicro grinding process: by the solution obtained after hydrolysis, with high-speed shearing emulsion machine process 10-20min, rotating speed is 8000-12000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 80-120MPa, number of processes is 2-3 time, obtains paste serous material;
4) fermenting: by step 3) paste serous material that obtains is through ultra high temperature short time sterilization, then adjust pH6.5-7.0, by volume percentage is that ripe lactic acid bacteria liquid spawn is cultivated in 10-15% access, puts into fermentation cylinder for fermentation, fermentation temperature at 35-37 DEG C, fermentation time 8-10h;
5) HIGH PRESSURE TREATMENT: by step 4) zymotic fluid that obtains through ultra high temperature short time sterilization, put into pressure pan, be forced into 3kgf/cm
2, and keep 10-20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter;
6) post processing: by step 5) filtrate that obtains, centrifugal, precipitation packs to obtain finished product after drying.
The preferred lactobacillus bulgaricus of lactic acid bacteria of the present invention, lactobacillus acidophilus, streptococcus thermophilus, streptococcus cremoris, bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum.
Described centrifugal, preferably 30 minutes, rotating speed 4000r/min.
Adopt method of the present invention with new Fresh squid for raw material extracts squid polypeptide, compared with prior art, advantage of the present invention:
1, the present invention is by using high pressure microjet superfine communication technique process squid raw material, high pressure microjet Ultra-Micro Grinding Equipment is utilized to produce extruding force, shearing force, impulsive force, through liquid-liquid, liquid-solid between high-speed impact, high shear, cavitation etc., the molecular structure of material is changed.High pressure microjet Ultra-Micro Grinding Equipment can accomplish nano-dispersed, thinning effect can reach 0.lum even below, pressure is up to 100-200MPa, the solution particles even particle size distribution obtained, have simple to operate, pollution-free, can online cleaning-sterilizing, high efficiency can carry out the plurality of advantages such as breaking-wall cell.
2, prior art is generally adopt enzymolysis to the process of squid raw material, enzymolysis can only process albumen, and the present invention is processed by lactic acid bacteria, there is the decomposition promoting protein, monose, and promote the absorption of the nutriment such as calcium, magnesium, produce a large amount of benefit materials such as vitamin B complex, intestinal beneficial flora can also be increased, improve human gastrointestinal tract function, recover colony balance in human body intestinal canal, form antibacterial biological barrier, suppress cholesterol absorption, reducing blood lipid, hypotensive activity, immunoregulation effect, strengthens the effect such as body immunity and resistance.
3, adopt method of the present invention, active peptide content is more than 95%, and small-molecular peptides ratio is more than 95%.
Detailed description of the invention
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
An extracting method for squid active peptide, comprises the steps:
1) Feedstock treating: rub after new Fresh squid is cleaned;
2) be hydrolyzed: the pure water adding 8 times amount, regulate mixture ph to 1.8, boil rear maintenance pressure 0.1Mpa and be hydrolyzed 10 hours, boil to maintain in press process and note supplementing the moisture lost;
3) high pressure microjet ultramicro grinding process: by the solution obtained after hydrolysis, with high-speed shearing emulsion machine process 10min, rotating speed is 12000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 80MPa, and number of processes is 3 times, obtains paste serous material;
4) fermenting: by step 3) paste serous material that obtains is through ultra high temperature short time sterilization, then adjust pH7.0, by volume percentage is that ripe lactobacillus bulgaricus liquid spawn is cultivated in 10% access, puts into fermentation cylinder for fermentation, fermentation temperature at 35-37 DEG C, fermentation time 8h;
5) HIGH PRESSURE TREATMENT: by step 4) zymotic fluid that obtains through ultra high temperature short time sterilization, put into pressure pan, be forced into 3kgf/cm
2, and keep 20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter;
6) post processing: by step 5) filtrate that obtains, centrifugal, precipitation packs to obtain finished product after drying.
Embodiment 2:
An extracting method for squid active peptide, comprises the steps:
1) Feedstock treating: rub after new Fresh squid is cleaned;
2) be hydrolyzed: the pure water adding 10 times amount, regulate mixture ph to 2.5, boil rear maintenance pressure 0.1Mpa and be hydrolyzed 8 hours, boil to maintain in press process and note supplementing the moisture lost;
3) high pressure microjet ultramicro grinding process: by the solution obtained after hydrolysis, with high-speed shearing emulsion machine process 20min, rotating speed is 10000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 100MPa, number of processes is 2 times, obtains paste serous material;
4) fermenting: by step 3) paste serous material that obtains is through ultra high temperature short time sterilization, then adjust pH6.5, by volume percentage is that ripe streptococcus thermophilus liquid spawn is cultivated in 12% access, puts into fermentation cylinder for fermentation, fermentation temperature at 35-37 DEG C, fermentation time 9h;
5) HIGH PRESSURE TREATMENT: by step 4) zymotic fluid that obtains through ultra high temperature short time sterilization, put into pressure pan, be forced into 3kgf/cm
2, and keep 15min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter;
6) post processing: by step 5) filtrate that obtains, centrifugal, precipitation packs to obtain finished product after drying.
Embodiment 3:
An extracting method for squid active peptide, comprises the steps:
1) Feedstock treating: rub after new Fresh squid is cleaned;
2) be hydrolyzed: the pure water adding 9 times amount, regulate mixture ph to 2.0, boil rear maintenance pressure 0.1Mpa and be hydrolyzed 10 hours, boil to maintain in press process and note supplementing the moisture lost;
3) high pressure microjet ultramicro grinding process: by the solution obtained after hydrolysis, with high-speed shearing emulsion machine process 15min, rotating speed is 8000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 120MPa, and number of processes is 3 times, obtains paste serous material;
4) fermenting: by step 3) paste serous material that obtains is through ultra high temperature short time sterilization, then adjust pH7.0, by volume percentage is that ripe bifidobacterium infantis liquid spawn is cultivated in 15% access, puts into fermentation cylinder for fermentation, fermentation temperature at 35-37 DEG C, fermentation time 10h;
5) HIGH PRESSURE TREATMENT: by step 4) zymotic fluid that obtains through ultra high temperature short time sterilization, put into pressure pan, be forced into 3kgf/cm
2, and keep 20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter;
6) post processing: by step 5) filtrate that obtains, centrifugal, precipitation packs to obtain finished product after drying.
Result:
Claims (2)
1. an extracting method for squid active peptide, is characterized in that, comprises the steps:
1) Feedstock treating: rub after new Fresh squid is cleaned;
2) be hydrolyzed: the pure water adding 8-10 times amount, regulate mixture ph to 1.8-2.5, boil rear maintenance pressure 0.1Mpa and be hydrolyzed 8-10 hour, boil to maintain in press process and note supplementing the moisture lost;
3) high pressure microjet ultramicro grinding process: by the solution obtained after hydrolysis, with high-speed shearing emulsion machine process 10-20min, rotating speed is 8000-12000r/min, then process in 25 DEG C with high pressure microjet Ultra-Micro Grinding Equipment, processing pressure is 80-120MPa, number of processes is 2-3 time, obtains paste serous material;
4) fermenting: by step 3) paste serous material that obtains is through ultra high temperature short time sterilization, then adjust pH6.5-7.0, by volume percentage is that ripe lactic acid bacteria liquid spawn is cultivated in 10-15% access, puts into fermentation cylinder for fermentation, fermentation temperature at 35-37 DEG C, fermentation time 8-10h;
5) HIGH PRESSURE TREATMENT: by step 4) zymotic fluid that obtains through ultra high temperature short time sterilization, put into pressure pan, be forced into 3kgf/cm
2, and keep 10-20min, make its pressure uniform, then make suddenly Pressure Drop to Okgf/cm second at 0-3
2, filter;
6) post processing: by step 5) filtrate that obtains, centrifugal, precipitation packs to obtain finished product after drying.
2. the extracting method of a kind of squid active peptide according to claim 1, is characterized in that: described lactic acid bacteria is lactobacillus bulgaricus, lactobacillus acidophilus, streptococcus thermophilus, streptococcus cremoris, bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054036A (en) * | 2013-02-07 | 2013-04-24 | 广东海洋大学 | Lactobacillus casei-containing fermented oyster powder |
| CN103614443A (en) * | 2013-11-22 | 2014-03-05 | 威海市宇王集团有限公司 | Method for extracting biological active peptide from marine aquatic product |
| CN103947818A (en) * | 2014-04-22 | 2014-07-30 | 钦州市山海生物科技有限公司 | Preparation method of squid active peptide |
| CN104137979A (en) * | 2014-07-29 | 2014-11-12 | 桂林实力科技有限公司 | Fructus momordicae aspartame and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054036A (en) * | 2013-02-07 | 2013-04-24 | 广东海洋大学 | Lactobacillus casei-containing fermented oyster powder |
| CN103614443A (en) * | 2013-11-22 | 2014-03-05 | 威海市宇王集团有限公司 | Method for extracting biological active peptide from marine aquatic product |
| CN103947818A (en) * | 2014-04-22 | 2014-07-30 | 钦州市山海生物科技有限公司 | Preparation method of squid active peptide |
| CN104137979A (en) * | 2014-07-29 | 2014-11-12 | 桂林实力科技有限公司 | Fructus momordicae aspartame and preparation method thereof |
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Application publication date: 20150617 |