CN115335387A - 核酸寡聚物的制造方法 - Google Patents

核酸寡聚物的制造方法 Download PDF

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Publication number
CN115335387A
CN115335387A CN202180023282.5A CN202180023282A CN115335387A CN 115335387 A CN115335387 A CN 115335387A CN 202180023282 A CN202180023282 A CN 202180023282A CN 115335387 A CN115335387 A CN 115335387A
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CN
China
Prior art keywords
group
formula
nucleic acid
formaldehyde
dichloroacetic acid
Prior art date
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Pending
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CN202180023282.5A
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English (en)
Chinese (zh)
Inventor
田中雄树
加纳俊史
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Sumitomo Chemical Co Ltd
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Sumitomo Chemical Co Ltd
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Publication date
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Publication of CN115335387A publication Critical patent/CN115335387A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
    • C07C51/44—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation by distillation
    • C07C51/46—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation by distillation by azeotropic distillation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Saccharide Compounds (AREA)
CN202180023282.5A 2020-03-27 2021-03-26 核酸寡聚物的制造方法 Pending CN115335387A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020058880 2020-03-27
JP2020-058880 2020-03-27
PCT/JP2021/013023 WO2021193954A1 (ja) 2020-03-27 2021-03-26 核酸オリゴマーの製造方法

Publications (1)

Publication Number Publication Date
CN115335387A true CN115335387A (zh) 2022-11-11

Family

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Family Applications (1)

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CN202180023282.5A Pending CN115335387A (zh) 2020-03-27 2021-03-26 核酸寡聚物的制造方法

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Country Link
US (1) US12522629B2 (https=)
EP (1) EP4130018A4 (https=)
JP (1) JP7698631B2 (https=)
KR (1) KR20220160010A (https=)
CN (1) CN115335387A (https=)
WO (1) WO2021193954A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117980285A (zh) * 2021-09-28 2024-05-03 住友化学株式会社 纯化二氯乙酸的制造方法
CN116333007B (zh) * 2021-12-23 2025-06-03 中国科学院化学研究所 一种基于点击化学的光响应性环形rna合成方法及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043694A1 (en) * 1998-02-26 1999-09-02 Isis Pharmaceuticals, Inc. Improved methods for synthesis of oligonucleotides
US20100331397A1 (en) * 2009-06-24 2010-12-30 Alios Biopharma, Inc. 2-5a analogs and their methods of use
CN103052711A (zh) * 2010-08-03 2013-04-17 株式会社博纳克 具有含氮脂环式骨架的单链核酸分子
CN106573011A (zh) * 2014-06-24 2017-04-19 艾丽奥斯生物制药有限公司 取代的核苷、核苷酸和其类似物
WO2021024465A1 (ja) * 2019-08-08 2021-02-11 住友化学株式会社 核酸分子の製造方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077709A (en) 1998-09-29 2000-06-20 Isis Pharmaceuticals Inc. Antisense modulation of Survivin expression
US6887990B1 (en) 1999-02-05 2005-05-03 Amersham Biosciences Corp Method for deprotecting oligonucleotides
US6261840B1 (en) 2000-01-18 2001-07-17 Isis Pharmaceuticals, Inc. Antisense modulation of PTP1B expression
AU2005275801B2 (en) 2004-08-26 2012-05-10 Nippon Shinyaku Co., Ltd. Phosphoramidite compound and method for producing oligo-RNA
ES2526295T5 (es) 2006-10-18 2021-05-04 Ionis Pharmaceuticals Inc Compuestos antisentido
EP2933333B1 (en) 2010-07-08 2018-02-21 Bonac Corporation Single-strand nucleic acid molecule for controlling gene expression
MX351566B (es) * 2011-06-21 2017-10-19 Akzo Nobel Chemicals Int Bv Proceso para separar acido monocloroacetico y acido dicloroacetico mediante destilacion extractiva.
MX370398B (es) * 2011-08-25 2019-12-11 Bonac Corp Compuesto de glicosido, metodo para producir tioeter, eter, metodo para producir eter, metodo para producir un compuesto de glicosido, metodo para producir acido nucleico.
CN104039961A (zh) 2012-01-07 2014-09-10 株式会社博纳克 具有氨基酸骨架的单链核酸分子
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
CA2969619A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
WO2016158809A1 (ja) 2015-03-27 2016-10-06 株式会社ボナック デリバリー機能と遺伝子発現制御能を有する一本鎖核酸分子
EP3328337B1 (en) 2015-07-31 2022-08-17 The Procter & Gamble Company Package of absorbent articles utilizing a shaped nonwoven
CN109072224B (zh) 2016-01-30 2022-07-15 株式会社博纳克 人工单导rna及其用途
JPWO2018182008A1 (ja) 2017-03-31 2020-02-06 株式会社ボナック 遺伝子発現制御機能を有する環状型核酸分子
BR112020005230A2 (pt) 2017-09-19 2020-09-24 Alnylam Pharmaceuticals, Inc. composições e métodos para o tratamento da amiloidose mediada por transtiretina (ttr)
US20210188895A1 (en) 2017-10-13 2021-06-24 Bonac Corporation Single-stranded nucleic acid molecule, and production method therefor
US11920131B2 (en) 2018-03-30 2024-03-05 Toray Industries, Inc. Method of producing hairpin single-stranded RNA molecule
KR102670605B1 (ko) 2018-04-24 2024-05-29 스미또모 가가꾸 가부시끼가이샤 아미다이트 화합물 및 그 화합물을 사용한 폴리뉴클레오티드의 제조 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043694A1 (en) * 1998-02-26 1999-09-02 Isis Pharmaceuticals, Inc. Improved methods for synthesis of oligonucleotides
US20100331397A1 (en) * 2009-06-24 2010-12-30 Alios Biopharma, Inc. 2-5a analogs and their methods of use
CN103052711A (zh) * 2010-08-03 2013-04-17 株式会社博纳克 具有含氮脂环式骨架的单链核酸分子
CN106573011A (zh) * 2014-06-24 2017-04-19 艾丽奥斯生物制药有限公司 取代的核苷、核苷酸和其类似物
WO2021024465A1 (ja) * 2019-08-08 2021-02-11 住友化学株式会社 核酸分子の製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CARLTON H. PAUL，等: "Acid binding and detritylation during oligonucleotide synthesis", 《NUCLEIC ACIDS RESEARCH》, vol. 24, no. 15, 31 December 1996 (1996-12-31), pages 3048 - 3052, XP055787716, DOI: 10.1093/nar/24.15.3048 *
MICHAEL SEPTAK: "Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis", 《NUCLEIC ACIDS RESEARCH》, vol. 24, no. 15, 31 December 1996 (1996-12-31), pages 3053 - 3058, XP055784527, DOI: 10.1093/nar/24.15.3053 *
SOHEIL POURSHAHIAN: "THERAPEUTIC OLIGONUCLEOTIDES, IMPURITIES, DEGRADANTS, AND THEIR CHARACTERIZATION BY MASS SPECTROMETRY", 《MASS SPECTROMETRY REVIEWS》, vol. 40, 16 December 2019 (2019-12-16), pages 75 *
蒋荣海: "《药物化学基础》", vol. 1, 31 May 2001, 中国医药科技出版社, pages: 106 *

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Publication number Publication date
US20230167152A1 (en) 2023-06-01
EP4130018A1 (en) 2023-02-08
KR20220160010A (ko) 2022-12-05
JPWO2021193954A1 (https=) 2021-09-30
WO2021193954A1 (ja) 2021-09-30
EP4130018A4 (en) 2024-04-17
US12522629B2 (en) 2026-01-13
JP7698631B2 (ja) 2025-06-25

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