CN115067431B

CN115067431B - Composition for improving intestinal function of dogs and cats, pet food and preparation method of pet food

Info

Publication number: CN115067431B
Application number: CN202210771696.2A
Authority: CN
Inventors: 巫鸿韵
Original assignee: Guangzhou Weinuo Animal Pharmaceutical Co ltd
Current assignee: Guangzhou Weinuo Animal Pharmaceutical Co ltd
Priority date: 2022-07-02
Filing date: 2022-07-02
Publication date: 2023-05-12
Anticipated expiration: 2042-07-02
Also published as: CN115067431A

Abstract

The invention discloses a composition for improving intestinal functions of dogs and cats, pet food and a preparation method thereof, wherein foods rich in nutrition, such as fresh chicken breast, duck meat, beef, fresh boneless salmon fillet, fresh potatoes, corns, black rice, tomatoes and the like, are selected as main nutrients of the pet food. The product solves the problem of low digestion and absorption rate of the traditional pet to the pet food, and the konjak polysaccharide, the lactobacillus acidophilus, the bifidobacterium longum and other foods prepared by the special process are added to prepare the microcapsule which can be used, so that the absorption capacity of the pet to the foods is fully improved, meanwhile, diarrhea and food allergy of the pet are pertinently managed, and the pet dogs and cats are helped to strengthen the immune system. In addition, probiotics are used as embedding wall materials of the microcapsules, so that the antiviral rate of the pet cats and dogs can be effectively improved, healthy intestinal walls can be established, and the probability of occurrence of diseases such as diarrhea and skin diseases related to antibiotics is reduced.

Description

Composition for improving intestinal function of dogs and cats, pet food and preparation method of pet food

Technical Field

The invention belongs to the field of pet foods, and particularly relates to a composition for improving intestinal functions of dogs and cats, pet food and a preparation method thereof.

Background

With the continuous progress of human society and the rapid development of technology, people are enthusiastically raising pet cats and dogs to improve life happiness, however, for pets with gastrointestinal functions frequently suffering from various problems, the fed food has a great influence on the state of the pet. The food is a high-grade animal food between human food and traditional livestock and poultry feed, and mainly aims to provide the most basic life guarantee and growth and development nutrition substances for animals and dogs, and the pet food is required to have the characteristics of comprehensive nutrition, high digestion and absorption rate, scientific formula, capability of treating or preventing diseases of cats and dogs and the like.

The pet food existing in the market at present generally contains the following substances: carbohydrates, proteins, fats, minerals, vitamins, water, and the like. The carbohydrate can provide glycogen needed by the body of the pet, provide energy for the body, maintain the brain cell function of the animal, prevent fat from being converted into energy only by keeping the provided energy sufficient, reduce the accumulation of fat metabolite ketone bodies and prevent ketoacidosis. Proteins are an important source of body tissues and somatic cells of pets, and muscles, nerves, connective tissues, glands, skin, hair, corners, blood and the like of animals take the proteins as main components to play a plurality of roles of conduction, transportation, support, protection, connection, exercise and the like, and in addition, the proteins play an important role in maintaining the normal distribution of osmotic pressure and moisture in the bodies of the animals; fat participates in important physiological activities of animal organisms and plays an important role in regulating anabolism of substances in vivo; the mineral substances are the most main components of somatic cells and tissues, especially forming bones, can regulate the constant osmotic pressure of animal blood and lymph, ensure that the cells obtain nutrition, maintain the acid-base balance of the blood, influence the solubility of other substances in the body, and have the functions of activating enzymes and hormones, thus being an indispensable nutrient substance for the growth and health maintenance of pets; animals require very small amounts of vitamins, often in milligrams or micrograms, but once deficient will cause the corresponding vitamin deficiency, causing health damage. Therefore, the prepared pet food needs balanced nutrition collocation, and the important nutrient substances need to be comprehensively, reasonably and scientifically proportioned.

Disclosure of Invention

Aiming at solving the problem of low digestion and absorption rate of the traditional pet to the pet food, the invention aims to provide the composition for improving the intestinal function of dogs and cats, which is added with probiotics such as bifidobacterium, lactobacillus acidophilus, bifidobacterium longum and the like, and the pet food.

A composition for improving intestinal functions of dogs and cats, pet food and a preparation method thereof are characterized in that: the konjak polysaccharide obtained after konjak tuber modification is selected as a microcapsule embedding wall material main body, and edible gelatin, bifidobacteria, lactobacillus acidophilus, bifidobacterium longum and the like are added to prepare the probiotic microcapsule embedding wall material, and then a plurality of food materials and stuffing with balanced nutrition are combined to prepare the pet food composition. The invention is realized by the following technical scheme:

s1, preparing a block food material: weighing 200-1000g of fresh chicken breast, 200-1000g of duck meat, 150-500g of beef, 150-300g of fresh deboned salmon fillet and 50-100g of fresh chicken heart, cleaning, cutting into 0.2cm blocks, adding 30-50mL of purified water, putting into a baking device, and baking at 90-100 ℃ for 7-10min for later use;

s2, preparing a coarse cereal mixture: weighing 300-500g of fresh potatoes, peeling and cutting the fresh potatoes into pieces, putting the pieces together with 300-600g of corns, 200-500g of black rice, 100-200g of red beans, 100-200g of eggs, 100-200g of purple potatoes and 200-500mL of purified water into a grinder, grinding the pieces into mud, filtering the mud with a 10-mesh filter screen to remove dregs and impurities, putting the filtered fluid raw mud into a curing machine, and curing at 90-105 ℃ for 5-15min;

s3, preparing vegetable and fruit puree stuffing: weighing 100-200g of tomatoes, 100-200g of rapes, 100-200g of baby cabbage, 100-200g of carrots, 50-100g of stoned apples, 50-100g of stoned nectarines and 200-500mL of purified water, putting into a grinder, stirring into mud, filtering dregs and impurities by using a filter screen with 10 meshes, putting into a curing machine for curing, wherein the temperature is controlled to be 90-105 ℃ and the curing time is 3-5min;

s4, preparing a probiotic microcapsule embedding wall material: weighing 50-100g of konjak tuber and 10-20mL of absolute ethyl alcohol, heating for 10-30min under the condition of constant temperature of 60-90 ℃ of a heatable magnetic stirrer, adding 500mL of 1mol/L hydrochloric acid solution, performing ultrasonic leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, performing ultrasonic leaching for 30-50min, and centrifuging by using a centrifuge under the conditions that: 4000-6000r/min,5-10min, heating supernatant to concentrate to 200mL to obtain konjak polysaccharide extract, adding edible gelatin 50-100g, bifidobacterium 5-10g, lactobacillus acidophilus 5-10g, bifidobacterium longum 5-10g, and purified water 30-50mL, stirring thoroughly for 5-10min, homogenizing in a homogenizer under the following conditions: the temperature is 30-45 ℃, the pressure is 10-30MPa, and the embedded wall material of the microcapsule is obtained after homogenization.

S5, embedding a compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit puree stuffing prepared in the steps S1, S2 and S3, adding 100-200g of salt, keeping the temperature at 20-30 ℃ for 10-20min, taking 1-5kg of homogenized stuffing and 0.5-1kg of probiotic microcapsule embedding wall material, and placing into a microcapsule embedding machine to prepare the probiotic microcapsule pet food with the length of 5 mm.

Preferably: the method comprises the following steps of S1 weighing 200g of fresh chicken breast, 200g of duck meat, 150g of beef, 150g of fresh boneless salmon fillet, 50g of fresh chicken heart and 30mL of purified water;

preferably: the fresh potato is 300g, the corn is 300g, the black rice is 200g, the red bean is 100g, the egg is 100g, the purple potato is 100g, and the purified water is 200mL;

preferably: the S3 is characterized in that the tomato is 100g, the rape is 100g, the baby cabbage is 100g, the carrot is 100g, the stoned apple is 50g, the stoned nectarine is 50g and the purified water is 200mL;

preferably: the S4 is characterized in that the konjak is 50g, the absolute ethyl alcohol is 10mL, the 1mol/L hydrochloric acid solution is 500mL, the 1mol/L potassium hydroxide solution is 500mL, the edible gelatin is 50g, the bifidobacterium is 5g, the lactobacillus acidophilus is 5g, the bifidobacterium longum is 5g, and the purified water is 30mL.

1. The pet food prepared by the invention is free from adding artificial preservative and antioxidant, and is gentle to intestines and stomach of pets.

2. The konjak polysaccharide, lactobacillus acidophilus and bifidobacterium longum prepared by the special process are selected as embedding wall materials of the microcapsule, the konjak polysaccharide modified by the special method can promote the peristalsis of intestines and stomach, promote the proliferation of lactobacillus acidophilus and bifidobacterium longum, and the synergistic effect of various probiotics and konjak polysaccharide in the intestines of the pet, can effectively improve the antiviral capability, immune function and the like of the cat and the dog of the pet, and help the cat and the dog to establish healthy intestinal walls, thereby reducing the occurrence probability of various diseases including diarrhea, skin diseases and the like related to antibiotics.

3. The invention has simple operation process, rich selected food raw materials and suitability for industrial-grade mass production.

4. The pet food disclosed by the invention is reasonable in formula, rich in nutrition and good in edible taste, and the variety of raw materials can meet the daily nutritional requirements of pets.

Drawings

FIG. 1 is a schematic illustration of a microcapsule embedding structure.

Figure 2 is a profile view of the pet food of the present invention.

FIG. 3 is a bar graph of protein digestibility of examples and comparative examples of the present invention.

Fig. 4 is a bar graph of fat digestibility for examples of the present invention and comparative examples.

FIG. 5 shows the molecular formula of konjac glucomannan prepared by the specific process of the present invention.

FIG. 6 shows nuclear magnetic resonance hydrogen spectrum of konjak polysaccharide prepared by special technique in the invention.

Detailed Description

Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention. It should be noted that the following embodiments and features in the embodiments may be combined with each other without conflict.

All probiotics of the invention are purified bacterial powder and are purchased in the market.

Example 1

S1, preparing a block food material: weighing 200g of fresh chicken breast, 200g of duck, 150g of beef, 150g of fresh deboned salmon fillet and 50g of fresh chicken heart, cleaning, cutting into 0.2cm blocks, adding 30mL of purified water, putting into a baking device, and baking at 90 ℃ for 7min for later use;

s2, preparing a coarse cereal mixture: weighing 300g of fresh potatoes, peeling, cutting into pieces, putting 300g of corn, 200g of black rice, 100g of red bean, 100g of egg, 100g of purple potato and 200mL of purified water into a grinder, grinding into mud, filtering residues and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, and curing at 90 ℃ for 5min;

s3, preparing vegetable and fruit puree stuffing: weighing 100g of tomatoes, 100g of rape, 100g of baby cabbage, 100g of carrots, 50g of stoned apples, 50g of stoned nectarines and 200mL of purified water, putting into a grinder, stirring into mud, filtering out dregs and impurities by using a filter screen with 10 meshes, and then putting into a curing machine for curing at the temperature of 90 ℃ for 3min;

s4, preparing a probiotic microcapsule embedding wall material: weighing 50g of konjak tuber and 10mL of absolute ethyl alcohol, heating for 10min at the constant temperature of 90 ℃ by a heatable magnetic stirrer, adding 500mL of 1mol/L hydrochloric acid solution, performing ultrasonic leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, performing ultrasonic leaching for 30min, and centrifuging by using a centrifuge under the following conditions: 4000r/min,5min, heating supernatant to concentrate to 200mL to obtain konjak polysaccharide extract, adding edible gelatin 50g, bifidobacterium 5g, lactobacillus acidophilus 5g, bifidobacterium longum 5g and purified water 30mL, stirring thoroughly for 5min, homogenizing in a homogenizer under the following specific conditions: homogenizing at 30deg.C under 10MPa to obtain microcapsule embedded wall material;

s5, embedding a compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit puree stuffing prepared in the steps S1, S2 and S3, adding 100g of salt, keeping the temperature at 20 ℃ for 10min, taking 1kg of homogenized stuffing and 0.5kg of probiotic microcapsule embedding wall material, and putting into a microcapsule embedding machine to prepare the probiotic microcapsule pet food with the length of 5 mm.

Comparative example 1 the same procedure as in example 1 was repeated except that the distilled water was replaced with hydrochloric acid and potassium hydroxide solution in the same ratio at the time of treating konjak in step S4.

Comparative example 2 the same procedure as in example 1 was followed except that lactobacillus acidophilus was not added in step S3.

Comparative example 3 the parameters were the same as in example 1 except that bifidobacteria were not lengthened in step S3.

Open field trial is a commonly used animal behavioural experiment, and is an experimental method for evaluating the autonomous behaviors of experimental animals and researching the stress of animal behaviors. Animals move mainly in the peripheral area in a new open environment, and the number of activities in the central position is smaller, but the psychological exploration mode of the animals enables the animals to generate motivations for the activities in the central area, so that anxiety psychology generated by the animals can be observed. The experiment mainly explores the influence effect of modified konjak polysaccharide and probiotics on animal mental excitement, adopts 50 experimental subjects of purchased adult male mice for the same variety experiment, has the weight of 200+/-25 g, is divided into 5 groups of ten mice each, and has the specific feeding conditions that: the temperature is 20-25 ℃, the humidity is 50% -60%, 5g of different embedding wall materials containing konjak polysaccharide are respectively fed per day according to groups, the first group feeds the microcapsule embedding wall materials of modified konjak polysaccharide prepared according to the step S4 in the embodiment 1, the second group feeds the microcapsule embedding wall materials of non-modified konjak polysaccharide prepared according to the step S4 in the comparative example 1, the third group feeds the microcapsule embedding wall materials prepared according to the step S4 in the comparative examples 2 and 3, the fourth group feeds the microcapsule embedding wall materials, and the fifth group does not feed the microcapsule embedding wall materials, and the microcapsule embedding wall materials are used as blank control groups and are subjected to open field experimental test after being subjected to adaptive feeding for 60 days.

(1) Open field test box preparation

The open field experiment case is square case, and the material is medical ABS material, wherein: experiment box specification (long x wide x high): 100 cm.times.100 cm.times.40 cm, before the experiment starts, the experiment box is clean and odorless, and meanwhile, the SuperMaze animal behavior analysis software/visual track software, a camera and an experiment special computer are required to be prepared for data recording and analysis.

(2) Experimental area division

The experimental area is divided into four corners, four sides and a central area, the software automatically counts the movement path, the residence time, the entering times and the average speed of experimental animals in the areas, the movement condition of the reactive mice can be judged according to the total horizontal movement distance obtained by the test, and the horizontal movement distance of the normal depressed mice is very small and is mostly kept away from one corner of an open field. The anxiety condition of the mice can be reflected according to the total number of times of carrying in the central area obtained by the test, and the number of times of carrying in the central area of the depressed rats can be obviously reduced.

(3) Open field experimental test

After setting relevant parameters in experimental software, recording the number and date of animals, taking out the experimental animals from a raising cage respectively, rapidly placing the experimental animals in the central area of an experimental box back to an experimenter, immediately leaving the experimental box, recording the activities of the animals by using animal behavioural analysis software, continuously testing 1 mouse each time for 15 minutes, avoiding the mutual interference among individuals, placing the mice into the prepared raising cage after the experiment is finished, deodorizing by using an alcohol spraying instrument, wiping the animals with other numbers, and repeating the experiment by using the animals with other numbers.

Table 1 is a data record table for open field experiments. As can be seen from the data in the table, the average number of times the mice of the first group entered the central region and the average time period the mice were located in the central region were higher than those of the mice of the second group to the fifth group. This indicates that the mice in the first group are in the excitatory phase for a higher period of time than the other groups. This demonstrates that the samples prepared in example 1 have a significant anxiety-improving effect on mice, and that the average number of times the mice in the second to fifth groups entered the central region and the average length of time the mice were located in the central region were lower than those in the first group, indicating that neither the modified konjac polysaccharide alone nor the probiotic bacteria alone nor the unmodified konjac polysaccharide could achieve the effect of the samples prepared in example 1. It is shown that the modified konjak polysaccharide can only play a role in improving anxiety of mice when the modified konjak polysaccharide plays a role in simultaneously playing a role with two probiotics. It is hypothesized that the modified konjac polysaccharide has effects of including prebiotics and promoting secretion of certain active metabolites of probiotics (such as organic acids can inhibit growth and reproduction of pathogenic bacteria), and complex regulation of metabolism by interaction between two probiotics is not excluded, and finally, the brain intestinal axis of animals is affected, thereby achieving the effect of alleviating anxiety.

TABLE 1

Group of mice	Average number of times/times of entry of mice into central region	Average duration/min of mice in central region
			First group of	8.2±0.3	5.1±0.2
Second group of	5.1±0.2	2.8±0.4
			Third group of	6.0±0.1	3.0±0.3
Fourth group	4.8±0.3	2.5±0.1
			Fifth group of	3.3±0.4	2.0±0.2

Example 2

S1, preparing a block food material: weighing 1000g of fresh chicken breast, 1000g of duck meat, 500g of beef, 300g of fresh deboned salmon fillet and 100g of fresh chicken heart, cleaning, cutting into 0.2cm blocks, adding 50mL of purified water, putting into a baking device, and baking at 100 ℃ for 10min for later use;

s2, preparing a coarse cereal mixture: weighing 500g of fresh potatoes, peeling, cutting into pieces, putting 600g of corns, 500g of black rice, 200g of red beans, 200g of eggs, 200g of purple potatoes and 500mL of purified water into a grinder, grinding into mud, filtering residues and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, and curing at 105 ℃ for 15min;

s3, preparing vegetable and fruit puree stuffing: weighing 200g of tomatoes, 200g of rape, 200g of baby cabbage, 200g of carrots, 100g of stoned apples, 100g of stoned nectarines and 500mL of purified water, putting into a grinder, stirring into mud, filtering out dregs and impurities by using a filter screen with 10 meshes, and then putting into a curing machine for curing at the temperature of 105 ℃ for 5min;

s4, preparing a probiotic microcapsule embedding wall material: weighing 100g of konjak tuber and 20mL of absolute ethyl alcohol, heating for 10min under the condition that a heatable magnetic stirrer is kept at a constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, performing ultrasonic leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, performing ultrasonic leaching for 30min, and centrifuging by using a centrifuge under the condition that: 4000r/min,5min, heating supernatant to concentrate to 200mL to obtain konjak polysaccharide extract, adding edible gelatin 100g, bifidobacterium 10g, lactobacillus acidophilus 10g, bifidobacterium longum 10g and purified water 50mL, stirring thoroughly for 10min, homogenizing in a homogenizer under the following specific conditions: homogenizing at 45deg.C under 30MPa to obtain microcapsule embedded wall material;

s5, embedding a compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit puree stuffing prepared in the steps S1, S2 and S3, adding 200g of salt, keeping the temperature at 30 ℃ for 20min, taking 5kg of homogenized stuffing and 1kg of probiotic microcapsule embedding wall material, and putting into a microcapsule embedding machine to prepare the probiotic microcapsule pet food with the length of 5 mm.

Comparative example 4 the parameters were the same as in example 2 except that bifidobacterium was not added in step S1.

Comparative example 5 the same procedure as in example 2 was followed except that lactobacillus acidophilus was not added in step S1.

Example 3

S1, preparing a block food material: weighing 500g of fresh chicken breast, 500g of duck, 250g of beef, 200g of fresh deboned salmon fillet and 65g of fresh chicken heart, cleaning, cutting into 0.2cm blocks, adding 40mL of purified water, putting into a baking device, and baking at 92 ℃ for 8min for later use;

s2, preparing a coarse cereal mixture: weighing 350g of fresh potatoes, peeling, cutting into pieces, putting 400g of corns, 300g of black rice, 130g of red beans, 130g of eggs, 130g of purple potatoes and 300mL of purified water into a grinder, grinding into mud, filtering residues and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, and curing at the temperature of 95 ℃ for 8min;

s3, preparing vegetable and fruit puree stuffing: weighing 130g of tomatoes, 130g of rape, 130g of baby cabbage, 130g of carrots, 65g of stoned apples, 65g of stoned nectarines and 300mL of purified water, putting into a grinder, stirring into mud, filtering out dregs and impurities by using a filter screen with 10 meshes, and putting into a curing machine for curing at the temperature of 95 ℃ for 4min;

s4, preparing a probiotic microcapsule embedding wall material: weighing 65g of konjak tuber and 13mL of absolute ethyl alcohol, heating for 10min at the constant temperature of 90 ℃ by a heatable magnetic stirrer, adding 500mL of 1mol/L hydrochloric acid solution, performing ultrasonic leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, performing ultrasonic leaching for 30min, and centrifuging by using a centrifuge under the following conditions: 4000r/min,5min, heating supernatant to concentrate to 200mL to obtain konjak polysaccharide extract, adding edible gelatin 65g, bifidobacterium 6.5g, lactobacillus acidophilus 6.5g, bifidobacterium longum 6.5g and purified water 38mL, stirring thoroughly for 6.5min, homogenizing in a homogenizer under the following specific conditions: homogenizing at 35deg.C under 20MPa to obtain microcapsule embedded wall material;

s5, embedding a compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit puree stuffing prepared in the steps S1, S2 and S3, adding 130g of salt, keeping the temperature at 23 ℃ for 13min, taking 2kg of homogenized stuffing and 0.7kg of probiotic microcapsule embedding wall material, and putting into a microcapsule embedding machine to prepare the probiotic microcapsule pet food with the length of 5 mm.

Comparative example 6 the same parameters as in example 3 were followed except that bifidobacteria and lactobacillus acidophilus were not added in step S2.

Comparative example 7 the same parameters as in example 3 were followed except that bifidobacteria and longum were not added in step S2.

Example 4

S1, preparing a block food material: weighing 800g of fresh chicken breast, 800g of duck meat, 400g of beef, 250g of fresh deboned salmon fillet and 85g of fresh chicken heart, cleaning, cutting into 0.2cm blocks, adding 45mL of purified water, putting into a baking device, and baking at 96 ℃ for 9min for later use;

s2, preparing a coarse cereal mixture: weighing 450g of fresh potatoes, peeling, cutting into pieces, putting 500g of corns, 400g of black rice, 170g of red beans, 170g of eggs, 170g of purple potatoes and 400mL of purified water into a grinder, grinding into mud, filtering residues and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, and curing at the temperature of 100 ℃ for 12min;

s3, preparing vegetable and fruit puree stuffing: weighing 170g of tomatoes, 170g of rape, 170g of baby cabbage, 170g of carrots, 90g of stoned apples, 90g of stoned nectarines and 400mL of purified water, putting into a grinder, stirring into mud, filtering out dregs and impurities by using a filter screen with 10 meshes, and putting into a curing machine for curing at the temperature of 100 ℃ for 4.5min;

s4, preparing a probiotic microcapsule embedding wall material: weighing 85g of konjak tuber and 17mL of absolute ethyl alcohol, heating for 10min at the constant temperature of 90 ℃ by a heatable magnetic stirrer, adding 500mL of 1mol/L hydrochloric acid solution, performing ultrasonic leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, performing ultrasonic leaching for 30min, and centrifuging by using a centrifuge under the following conditions: 4000r/min,5min, heating supernatant to concentrate to 200mL to obtain konjak polysaccharide extract, adding edible gelatin 85g, bifidobacterium 9g, lactobacillus acidophilus 9g, bifidobacterium longum 9g and purified water 45mL, stirring thoroughly for 9min, homogenizing in a homogenizer under the following specific conditions: homogenizing at 40deg.C under 26MPa to obtain microcapsule embedded wall material;

s5, embedding a compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit puree stuffing prepared in the steps S1, S2 and S3, adding 170g of salt, keeping the temperature at 28 ℃ for 17min, taking 4kg of homogenized stuffing and 0.8kg of probiotic microcapsule embedding wall material, and putting into a microcapsule embedding machine to prepare the probiotic microcapsule pet food with the length of 5 mm.

Comparative example 8 the same parameters as in example 4 were followed except that in step S4 the edible gelatin was changed to a gelatin.

Edible gelatin is a hydrolysis product of collagen, belongs to a fat-free cholesterol-free high protein, and is a natural nutritional food thickener. Because the edible gelatin is taken as a powerful protective colloid, the emulsifying power is strong, and the coagulation of proteins such as milk, soybean milk and the like caused by the action of gastric acid can be inhibited after the edible gelatin enters the stomach, so that the edible gelatin is beneficial to food digestion, and the viscosity of the microcapsule can be effectively improved by adding the edible gelatin into the microcapsule embedding wall material, and the embedding effect is ensured.

Digestibility means the percentage of ingested material that is determined by a digestion test as a parameter that determines digestibility, since only a portion of the nutrients entering the digestive system can be absorbed. We can measure the amount of nutrients contained in both feed and faeces and calculate the difference between the two fractions, which is the fraction digested and absorbed by the pet, usually expressed as a percentage or in small numbers for 1 (1 means complete digestion).

180 Chinese rural dogs with weights of 10kg plus or minus 0.5kg and ages of 2-3 years are selected and divided into 12 groups, the groups have no statistical difference, the compositions prepared in the invention in the embodiment 1-4 are fed in the embodiment 1-4, the compositions prepared in the invention in the comparative embodiment 1-8 are fed in the group 1-8, the daily feeding amount is 50g/kg, free water is adopted, and the feeding conditions are the same: the Chinese gardens are fed in the same environment with the temperature kept at 20-25 ℃ and the humidity kept at 50-60%, feces are taken after 3 days of feeding, the protein residual quantity and the fat residual quantity in the feces are tested, and the digestion and absorption rate results are shown in Table 2 and Table 3.

TABLE 2

Test sample	Protein content in pet food	Fecal-like protein content	Digestion and absorption rate
				Example 1	0.85±0.12	0.76±0.14	0.894±0.012
Example 2	0.83±0.16	0.74±0.13	0.891±0.015
				Example 3	0.83±0.12	0.71±0.15	0.855±0.020
Example 4	0.84±0.08	0.71±0.17	0.845±0.019
				Comparative example 1	0.81±0.06	0.64±0.08	0.790±0.018
Comparative example 2	0.80±0.21	0.65±0.02	0.800±0.022
				Comparative example 3	0.81±0.11	0.64±0.06	0.790±0.026
Comparative example 4	0.75±0.15	0.60±0.07	0.800±0.019
				Comparative example 5	0.73±0.21	0.59±0.02	0.808±0.029
Comparative example 6	0.70±0.09	0.58±0.05	0.828±0.027
				Comparative example 7	0.77±0.07	0.61±0.01	0.792±0.016
Comparative example 8	0.78±0.13	0.66±0.01	0.846±0.023

The experimental results in Table 2 and FIG. 4 show that the pet food of example 1 has the highest protein content in the original pet food and the highest digestibility of the pet dogs, compared with examples 2-4 and comparative examples 1-8, which means that example 1 can provide the pet with more nutritious food, improve the absorption efficiency of the pet on the food, and reveal that the pet food provided by the invention has a certain help for improving the digestion and absorption capacity of the pet.

The digestibility of pet food (for example dogs) simply reflects the ability of the food ingested by dogs to deliver basic nutrients, which affects not only the stool volume and quality (how much the dog is pulled, and the nature and smell of the stool), but most importantly, the digestibility of pet food affects the long-term health of the dog. Generally, dog food has a digestibility of 75% or less, and its quality is relatively low; the digestibility is 75% -82%, which belongs to medium quality; the digestibility is higher than 82 percent, belonging to high-quality food.

TABLE 3 Table 3

Test sample	Fat content in pet food	Fat content of faeces	Digestion and absorption rate
				Example 1	0.54±0.003	0.45±0.009	0.833±0.012
Example 2	0.52±0.013	0.43±0.008	0.827±0.015
				Example 3	0.53±0.011	0.39±0.003	0.736±0.020
Example 4	0.51±0.008	0.38±0.002	0.745±0.019
				Comparative example 1	0.48±0.012	0.34±0.007	0.708±0.018
Comparative example 2	0.49±0.009	0.36±0.009	0.734±0.022
				Comparative example 3	0.50±0.007	0.35±0.007	0.700±0.026
Comparative example 4	0.42±0.008	0.31±0.008	0.738±0.019
				Comparative example 5	0.43±0.012	0.30±0.008	0.698±0.029
Comparative example 6	0.40±0.011	0.31±0.007	0.775±0.027
				Comparative example 7	0.48±0.008	0.38±0.006	0.791±0.016
Comparative example 8	0.47±0.007	0.34±0.008	0.723±0.023

The experimental results in table 3 and fig. 4 show that, compared with examples 2-4 and comparative examples 1-8, the pet food of example 1 has the highest fat content, and the digestion and absorption rate of pet dogs is as high as 83.3%, and the pet food belongs to high-quality pet foods, and the data can indicate that example 1 can provide more fat for pets, can improve the gastrointestinal functions of the pets, and accelerate the absorption efficiency of the foods.

From tables 2 and 3, the digestibility of protein and fat was higher in the example group than in the comparative example group, and the digestibility of the comparative examples 4 to 7 was significantly lower than in the example group, which indicates that there was a synergistic effect among bifidobacterium, lactobacillus acidophilus and bifidobacterium longum added in the example group, resulting in an improved absorption efficiency of fat and protein by the intestinal tract of the well-known dogs in the China field. The reason is probably that the konjak polysaccharide modified by the special method can provide higher nutrition level, promote the proliferation of lactobacillus acidophilus and bifidobacterium longum, and the increase of the content of probiotics in the intestinal tract of the pet can effectively improve the antiviral rate of cats and dogs of the pet, and help the dogs to build healthy intestinal wall, thereby reducing the probability of diseases such as diarrhea, skin diseases and the like related to antibiotics.

Gastrointestinal push function detection

The animals of Kunming mice were taken and fasted for 20-2 hours and randomly divided into 3 groups, namely a control group, a group of example 1, a group of morpholine, 10 animals each, labeled with picric acid. The samples (20.0 mg/kg) and the morpholine solution (3.9 mg/kg) of example 1 were each administered to the group for the administration, the control group was administered with an equivalent amount of physiological saline for the administration, and after 90 minutes each group was administered with 0.3mL of ink for the administration. After 20 minutes, cervical vertebra is killed, the abdominal cavity is opened to separate the intestinal membrane, the intestinal canal is sheared from the upper end to the pylorus and from the lower end to the ileocecum, the intestinal canal is placed on a tray to gently pull the small intestine into a straight line, and the length of the intestinal canal is measured to be the total length of the small intestine. The distance from the pylorus to the leading edge of the ink is taken as the "ink advance distance in the intestine". The percentage of ink advanced was calculated using the formula. Ink advance (%) =distance of advance of ink in the intestine (cm)/total length of small intestine (cm) ×100%. The comparison results of the small intestine pushing functions of the mice in each group are shown in Table 4.

TABLE 4 Table 4

From experimental data, the rate of promoting gastrointestinal peristalsis of the example 1 group is not as same as that of the morpholine group, but the data of the example 1 group is compared with that of a blank control group, so that the pet food prepared by the invention has a certain effect of promoting gastrointestinal peristalsis. The reason for the conjecture may be that the non-ulcer dyspepsia of the pet is caused by bad moods such as tension, anxiety, dysphoria and the like. The pet food prepared by the invention improves anxiety of pets by mixing the modified konjak polysaccharide and the probiotics, and helps the pets to build healthy gastrointestinal walls at the same time, thereby playing a role in promoting gastrointestinal peristalsis and further treating non-ulcer dyspepsia.

The above examples merely illustrate specific embodiments of the invention, which are described in more detail and are not to be construed as limiting the scope of the invention. It should be noted that, it should be understood that those skilled in the art should not depart from the spirit and principle of the present invention, any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention are all equivalents to be included in the scope of the present invention.

Claims

1. A preparation method of pet food for improving intestinal functions of dogs and cats is characterized by comprising the following steps: s1, preparing a block food material: weighing 200-1000g of fresh chicken breast, 200-1000g of duck meat, 150-500g of beef, 150-300g of fresh deboned salmon fillet and 50-100g of fresh chicken heart, cleaning, cutting into 0.2cm blocks, adding 30-50mL of purified water, putting into a baking device, and baking at 90-100 ℃ for 7-10min for later use; s2, preparing a coarse cereal mixture: weighing 300-500g of fresh potatoes, peeling and cutting the fresh potatoes into pieces, putting the pieces together with 300-600g of corns, 200-500g of black rice, 100-200g of red beans, 100-200g of eggs, 100-200g of purple potatoes and 200-500mL of purified water into a grinder, grinding the pieces into mud, filtering the mud with a 10-mesh filter screen to remove dregs and impurities, putting the filtered fluid raw mud into a curing machine, and curing at 90-105 ℃ for 5-15min; s3, preparing vegetable and fruit puree stuffing: weighing 100-200g of tomatoes, 100-200g of rapes, 100-200g of baby cabbage, 100-200g of carrots, 50-100g of stoned apples, 50-100g of stoned nectarines and 200-500mL of purified water, putting into a grinder, stirring into mud, filtering dregs and impurities by using a filter screen with 10 meshes, putting into a curing machine for curing, wherein the temperature is controlled to be 90-105 ℃ and the curing time is 3-5min; s4, preparing a probiotic microcapsule embedding wall material: weighing 50-100g of konjak tuber and 10-20mL of absolute ethyl alcohol, heating for 10min under the condition that a heatable magnetic stirrer is kept at a constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, performing ultrasonic leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, performing ultrasonic leaching for 30min, and centrifuging by using a centrifuge under the conditions that: 4000r/min,5min, heating supernatant to concentrate to 200mL to obtain konjak polysaccharide extract, adding edible gelatin 50-100g, bifidobacterium 5-10g, lactobacillus acidophilus 5-10g, bifidobacterium longum 5-10g and purified water 30-50mL, stirring thoroughly for 5-10min, homogenizing in a homogenizer under the following specific conditions: homogenizing at 30-45deg.C and 10-30MPa to obtain microcapsule embedded wall material; s5, embedding a compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit puree stuffing prepared in the steps S1, S2 and S3, adding 100-200g of salt, keeping the temperature at 20-30 ℃ for 10-20min, taking 1-5kg of homogenized stuffing and 0.5-1kg of probiotic microcapsule embedding wall material, and placing into a microcapsule embedding machine to prepare the probiotic microcapsule pet food with the length of 5 mm.

2. The method for preparing the pet food for improving the intestinal functions of dogs and cats according to claim 1, wherein the method comprises the following steps of: the fresh chicken breast meat weighed in the S1 is 200g, the duck meat is 200g, the beef is 150g, the fresh boneless salmon fillet is 150g, the fresh chicken heart is 50g, and the purified water is 30mL.

3. The method for preparing the pet food for improving the intestinal functions of dogs and cats according to claim 1, wherein the method comprises the following steps of: the fresh potato weighed in the S2 is 300g, the corn is 300g, the black rice is 200g, the red bean is 100g, the egg is 100g, the purple potato is 100g, and the purified water is 200mL.

4. The method for preparing the pet food for improving the intestinal functions of dogs and cats according to claim 1, wherein the method comprises the following steps of: the tomato is 100g, the rape is 100g, the baby cabbage is 100g, the carrot is 100g, the stoned apple is 50g, the stoned nectarine is 50g, and the purified water is 200mL.

5. The method for preparing the pet food for improving the intestinal functions of dogs and cats according to claim 1, wherein the method comprises the following steps of: the S4 is characterized in that the weighed konjak tubers are 50g, 10mL of absolute ethyl alcohol, 500mL of 1mol/L hydrochloric acid solution, 500mL of 1mol/L potassium hydroxide solution, 50g of edible gelatin, 5g of bifidobacterium, 5g of lactobacillus acidophilus, 5g of bifidobacterium longum and 30mL of purified water.

6. The method for preparing the pet food for improving the intestinal functions of dogs and cats according to claim 1, wherein the method comprises the following steps of: and S5, weighing 100g of salt, 1kg of stuffing and 0.5kg of probiotic microcapsule embedding wall material.

7. A pet food product made by the method of any one of claims 1 to 6.