CN115067431A

CN115067431A - Composition for improving intestinal function of dogs and cats, pet food and preparation method thereof

Info

Publication number: CN115067431A
Application number: CN202210771696.2A
Authority: CN
Inventors: 巫鸿韵
Original assignee: Guangzhou Weinuo Animal Pharmaceutical Co ltd
Current assignee: Guangzhou Weinuo Animal Pharmaceutical Co ltd
Priority date: 2022-07-02
Filing date: 2022-07-02
Publication date: 2022-09-20
Anticipated expiration: 2042-07-02
Also published as: CN115067431B

Abstract

The invention discloses a composition for improving intestinal functions of dogs and cats, pet food and a preparation method thereof. The product solves the problem of low digestibility of the traditional pet to pet food, and the food such as konjac polysaccharide, lactobacillus acidophilus, bifidobacterium longum and the like prepared by a special process is added and prepared into a usable microcapsule, so that the absorption capacity of the pet to the food is fully improved, the diarrhea and food allergy of the pet are managed in a targeted manner, and the immune system of the pet dog or cat is enhanced. In addition, probiotics are used as the embedding wall material of the microcapsules, so that the antiviral rate of the pet cat and dog can be effectively improved, the healthy gastrointestinal wall can be established, and the probability of diseases such as antibiotic-related diarrhea and skin diseases is reduced.

Description

Composition for improving intestinal function of dogs and cats, pet food and preparation method thereof

Technical Field

The invention belongs to the field of pet foods, and particularly relates to a composition for improving intestinal functions of dogs and cats, pet food and a preparation method thereof.

Background

With the continuous progress of human society and the rapid development of science and technology, people are interested in raising the happiness of the life of pet cats and dogs at present, however, for pets with various problems of gastrointestinal functions, the fed food has great influence on the state of the pets. The cat and dog food is a nutritional food specially provided for cats and dogs, belongs to a high-grade animal food between human food and traditional livestock and poultry feed, and mainly aims to provide nutrients for ensuring the most basic life and growth and development of the animals, and the pet food is required to have the characteristics of comprehensive nutrition, high digestibility, scientific formula, capability of treating or preventing diseases of the cats and dogs and the like.

The pet food currently available on the market generally contains the following substances: carbohydrates, proteins, fats, minerals, vitamins, water, and the like. Carbohydrates can provide glycogen required by the pet body, provide energy for the pet body, maintain the function of animal brain cells, prevent fat from being converted into energy, reduce the accumulation of fat metabolites and ketone bodies and prevent ketoacidosis only if the provided energy is kept sufficient. The protein is an important source of pet body tissues and body cell compositions, the muscle, nerve, connective tissue, gland, skin, hair, horn, blood and the like of the animal take the protein as a main component, and have multiple functions of conduction, transportation, support, protection, connection, movement and the like, and in addition, the protein also plays an important role in maintaining the osmotic pressure and the normal distribution of water in the animal body; fat participates in important physiological activities of animal organisms and plays an important regulation role in the anabolism of substances in vivo; the mineral is the main component of somatic cells and tissues, particularly forming bones, can regulate the constant osmotic pressure of animal blood and lymph, ensure that cells obtain nutrition, maintain the acid-base balance of blood, influence the solubility of other substances in vivo, simultaneously have the functions of activating enzyme and hormone, and are indispensable nutrient substances for pet growth and health maintenance; animals require very little vitamin, often in milligrams or micrograms, but in the absence of vitamin, the corresponding vitamin deficiency can cause health damage. Therefore, the prepared pet food needs balanced nutrition, and the important nutrients need to be comprehensively, reasonably and scientifically proportioned.

Disclosure of Invention

In order to solve the problem of low digestibility of the traditional pet food, the invention aims to provide the composition and the pet food for improving the intestinal function of the dogs and cats, which are added with probiotics such as bifidobacterium, lactobacillus acidophilus, bifidobacterium longum and the like, wherein the composition and the pet food can fully improve the absorption capacity of the pets on food, and simultaneously pertinently manage diarrhea and food allergy of the pets and help the dogs and cats of the pets to enhance the immune system.

A composition for improving the intestinal function of dogs and cats, pet food and a preparation method thereof are characterized in that: selecting konjac polysaccharide obtained by modifying konjac tubers as a main body of a microcapsule embedding wall material, adding substances such as edible gelatin, bifidobacterium, lactobacillus acidophilus, bifidobacterium longum and the like to prepare a probiotic microcapsule embedding wall material, and then combining multiple food materials and stuffing with balanced nutrition to prepare the pet food composition. The invention is realized by the following technical scheme:

s1, preparing the blocky food materials: weighing 1000g of fresh chicken breast 200-;

s2, preparing a coarse cereal mixture: weighing 500g of fresh potato, peeling and cutting into blocks, putting the blocks together with 600g of 300g of corn, 500g of black rice, 200g of red bean, 200g of egg, 200g of purple potato and 500mL of purified water into a grinder for grinding into mud, filtering dregs and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, wherein the curing temperature is 90-105 ℃, and the curing time is 5-15 min;

s3, preparing vegetable and fruit paste stuffing: weighing 200g of tomato, 200g of rape, 100g of baby cabbage, 200g of carrot, 100g of stone-removed apple, 50-100g of stone-removed nectarine and 500mL of purified water, putting into a grinder, grinding into mud, filtering out dregs and impurities by using a 10-mesh filter screen, and then putting into a curing machine for curing, wherein the temperature is controlled to be 90-105 ℃, and the curing time is 3-5 min;

s4, preparation of the probiotic microcapsule embedding wall material: weighing 50-100g of konjak tubers and 10-20mL of absolute ethanol, heating for 10-30min under the condition that a magnetic stirrer can be heated and the constant temperature is 60-90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, then adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30-50min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000-: the temperature is 30-45 ℃, the pressure is 10-30MPa, and the embedding wall material of the microcapsule is obtained after homogenization.

S5, embedding of the compound: homogenizing the blocky food materials, the coarse cereal mixture and the vegetable and fruit paste stuffing prepared by the steps of S1, S2 and S3, adding 100-200g of salt, keeping the temperature at 20-30 ℃ for 10-20min, taking 1-5kg of homogenized stuffing and 0.5-1kg of probiotic microcapsule embedding wall material, and putting the obtained materials into a microcapsule embedding machine to obtain the 5mm probiotic microcapsule pet food.

Preferably: 200g of fresh chicken breast, 200g of duck, 150g of beef, 150g of fresh boned salmon fillet, 50g of fresh chicken heart and 30mL of purified water, which are weighed in the S1;

preferably: 300g of fresh potatoes, 300g of corns, 200g of black rice, 100g of red beans, 100g of eggs, 100g of purple potatoes and 200mL of purified water in the S2;

preferably: 100g of tomatoes, 100g of rapes, 100g of baby cabbage, 100g of carrots, 50g of denucleated apples, 50g of denucleated nectarines and 200mL of purified water are weighed in the S3;

preferably: 50g of konjac, 10mL of absolute ethyl alcohol, 500mL of 1mol/L hydrochloric acid solution, 500mL of 1mol/L potassium hydroxide solution, 50g of edible gelatin, 5g of bifidobacterium, 5g of lactobacillus acidophilus, 5g of bifidobacterium longum and 30mL of purified water are weighed in the S4.

1. The pet food prepared by the invention does not contain artificial preservatives and antioxidants, and is mild to intestines and stomach of pets.

2. The konjac polysaccharide, the lactobacillus acidophilus and the bifidobacterium longum which are prepared by a special process are selected as the embedding wall materials of the microcapsules, the konjac polysaccharide modified by a special method can promote the peristalsis of intestines and stomach and the proliferation of the lactobacillus acidophilus and the bifidobacterium longum, and various probiotics and the konjac polysaccharide have synergistic effect in the intestinal tracts of pets, so that the antiviral capacity, the immune function and the like of the cats and dogs of pets can be effectively improved, and the healthy intestinal and gastric walls can be established by the aid of the probiotics and the konjac polysaccharide, so that the probability of occurrence of various diseases including antibiotic-related diarrhea, skin diseases and the like is reduced.

3. The invention has simple operation process and rich selected food raw materials, and is suitable for industrial-grade mass production.

4. The pet food disclosed by the invention is reasonable in formula, rich in nutrition and good in edible mouthfeel, and the variety of raw materials can meet the daily nutritional requirements of pets.

Drawings

FIG. 1 is a schematic view of an embedding structure of a microcapsule.

Fig. 2 is a shape diagram of the pet food of the present invention.

FIG. 3 is a histogram of the digestibility of proteins for examples of the present invention and comparative examples.

Fig. 4 is a bar graph of fat digestibility according to examples of the present invention and comparative examples.

FIG. 5 shows the molecular formula of konjac polysaccharides prepared by a special process of the present invention.

FIG. 6 is the hydrogen nuclear magnetic resonance spectrum of the konjac polysaccharide prepared by the special process of the invention.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It is to be noted that the features in the following embodiments and examples may be combined with each other without conflict.

All probiotics in the invention are purified bacterium powder and are purchased and obtained from the market.

Example 1

S1, preparing the blocky food materials: weighing 200g of fresh chicken breast, 200g of duck, 150g of beef, 150g of fresh boned salmon fillet and 50g of fresh chicken heart, cleaning, cutting into blocks of 0.2cm, adding 30mL of purified water, putting into a baking device, and drying at 90 ℃ for 7min for later use;

s2, preparing a coarse cereal mixture: weighing 300g of fresh potatoes, peeling, cutting into blocks, putting 300g of corns, 200g of black rice, 100g of red beans, 100g of eggs, 100g of purple potatoes and 200mL of purified water into a grinder, grinding into mud, filtering out dregs and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, wherein the curing temperature is 90 ℃, and the curing time is 5 min;

s3, preparing vegetable and fruit paste stuffing: weighing 100g of tomatoes, 100g of rapes, 100g of baby cabbage, 100g of carrots, 50g of denucleated apples, 50g of denucleated nectarines and 200mL of purified water, putting the materials into a grinder, stirring the materials into mud, filtering dregs and impurities by using a 10-mesh filter screen, and then putting the mud into a curing machine for curing, wherein the temperature is controlled at 90 ℃ and the curing time is 3 min;

s4, preparation of the probiotic microcapsule embedding wall material: weighing 50g of konjac tubers and 10mL of absolute ethyl alcohol, heating for 10min under the condition that a magnetic stirrer can be heated at a constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000r/min, 5min, taking supernatant, heating and concentrating to 200mL to obtain a konjac polysaccharide extract, then adding 50g of edible gelatin, 5g of bifidobacterium, 5g of lactobacillus acidophilus, 5g of bifidobacterium longum and 30mL of purified water, fully stirring for 5min, and then homogenizing in a homogenizer under the specific conditions as follows: the temperature is 30 ℃, the pressure is 10MPa, and the embedding wall material of the microcapsule is obtained after homogenization for standby;

s5, embedding of the compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit paste stuffing prepared by the steps of S1, S2 and S3, adding 100g of salt, keeping the temperature at 20 ℃ for 10min, putting 1kg of homogenized stuffing and 0.5kg of probiotic microcapsule embedding wall material into a microcapsule embedding machine, and preparing the 5mm probiotic microcapsule pet food.

Comparative example 1, the same parameters as in example 1 were used except that hydrochloric acid and a potassium hydroxide solution were replaced with distilled water in the same ratio in the treatment of konjak in step S4.

Comparative example 2, the same parameters as in example 1 except that Lactobacillus acidophilus was not added in step S3.

Comparative example 3, the parameters were the same as in example 1 except that the bifidobacterium was not lengthened in step S3.

The open field trial is a commonly used animal behavior experiment, and is an experimental method for evaluating the autonomous behavior of an experimental animal and researching the animal behavior tension. The animals mainly move in the peripheral area in the new and open environment, but the number of activities in the central area is small, but the psychological exploration mode of the animals causes the motivation of the activities in the central area, thereby observing the anxiety psychology generated by the animals. The experiment mainly explores the influence effect of the modified konjac polysaccharides and probiotics on the animal mental excitation, adopts 50 purchased adult male mice for the same variety experiment, has the weight of 200 +/-25 g, is divided into 5 groups of ten mice each, and has the following specific feeding conditions: the temperature is 20-25 ℃, the humidity is 50-60%, 5g of different embedding wall materials containing konjac polysaccharide are respectively fed to each group every day, the first group is fed with the modified konjac polysaccharide microcapsule embedding wall material prepared according to the step S4 in the example 1, the second group is fed with the non-modified konjac polysaccharide microcapsule embedding wall material prepared according to the step S4 in the comparative example 1, the third group and the fourth group are fed with the microcapsule embedding wall materials prepared according to the step S4 in the comparative examples 2 and 3, and the fifth group is not fed with the microcapsule embedding wall material and is used as a blank control group, and open field experiment tests are carried out after adaptive feeding is carried out for 60 days.

(1) Open field test box preparation

Spacious field experimental box is square case, and the material is medical ABS material, wherein: experimental box specifications (length, width, height): 100cm 40cm, before the experiment, the experimental box is confirmed to be clean and odorless, and SuperMaze animal behavior analysis software/VisuTrack software, a video camera and an experiment special computer are prepared for data recording and analysis.

(2) Experimental region partitioning

The experimental area is divided into four corners, four sides and a central area, software automatically counts the movement distance, detention time, entry times and average speed of the experimental animals in the areas, the movement condition of the mice can be judged and reflected according to the total horizontal movement distance obtained by testing, the horizontal movement distance of the mice in general depression is very small, and the mice mostly avoid the immobility of one corner in an open field. The anxiety condition of the mice can be reflected according to the total times of the central zone entry obtained by the test, and the entry times of the central zone of the depressed rats can be obviously reduced.

(3) Open field experimental testing

After setting up relevant parameters in the experimental software, record animal's serial number and date, take out the laboratory animal respectively from the rearging cage, place in laboratory box central area rapidly dorsad the laboratory technician, leave immediately, use animal behavior analysis software record animal's activity, the experimentation lasts 15 minutes, 1 mice of each test, avoid the mutual interference between the individual, after the experiment, put the mice into the rearging cage that prepares, remove the flavor with alcohol spraying instrument, use the paper handkerchief to wipe dry, use the animal repetition experiment of other numbers.

Table 1 is a data record table for the open field experiment. As can be seen from the data in the table, the average number of times the mice in the first group entered the central area and the average length of time the mice were located in the central area were higher than those in the second to fifth groups. This indicates that the mice in the first group were in the excitation phase for a longer period of time than the other groups. This indicates that the samples prepared in example 1 were significantly effective in improving anxiety in mice, and that the average number of times mice entered the central region and the average length of time mice were located in the central region was lower than those of the first group in the second to fifth groups, indicating that neither the modified konjac polysaccharides alone nor the probiotics alone nor the unmodified konjac polysaccharides could achieve the effect of the samples prepared in example 1. The modified konjac polysaccharide can only play a role in improving the anxiety of mice when playing a role with two probiotics. The modified konjac polysaccharide is suspected to have the effects of including prebiotics and promoting secretion of some active metabolites of the probiotics (for example, organic acid can inhibit growth and reproduction of pathogenic bacteria), and complex regulation and control effects of interaction between the two probiotics on metabolism are not excluded, and the brain and intestinal axes of animals are influenced finally, so that the effect of relieving anxiety is achieved.

TABLE 1

Group of mice	Average number of mice entering the central zone/time	Average length of time/min that mice were in the central zone
			First group	8.2±0.3	5.1±0.2
Second group	5.1±0.2	2.8±0.4
			Third group	6.0±0.1	3.0±0.3
Fourth group	4.8±0.3	2.5±0.1
			Fifth group	3.3±0.4	2.0±0.2

Example 2

S1, preparing the blocky food materials: weighing 1000g of fresh chicken breast, 1000g of duck, 500g of beef, 300g of fresh boned salmon fillet and 100g of fresh chicken heart, cleaning, cutting into blocks of 0.2cm, adding 50mL of purified water, putting into a baking device, and drying at 100 ℃ for 10min for later use;

s2, preparing a coarse cereal mixture: weighing 500g of fresh potatoes, peeling, cutting into blocks, putting 600g of corns, 500g of black rice, 200g of red beans, 200g of eggs, 200g of purple potatoes and 500mL of purified water into a grinder, grinding into mud, filtering out dregs and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, wherein the curing temperature is 105 ℃, and the curing time is 15 min;

s3, preparing vegetable and fruit paste stuffing: weighing 200g of tomatoes, 200g of rapes, 200g of baby cabbage, 200g of carrots, 100g of denucleated apples, 100g of denucleated nectarines and 500mL of purified water, putting the mixture into a grinder, stirring the mixture into mud, filtering dregs and impurities by using a 10-mesh filter screen, and then putting the mud into a curing machine for curing, wherein the temperature is controlled at 105 ℃, and the curing time is 5 min;

s4, preparation of the probiotic microcapsule embedding wall material: weighing 100g of konjac tubers and 20mL of absolute ethyl alcohol, heating for 10min under the condition that a magnetic stirrer can be heated at a constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000r/min, 5min, taking supernatant, heating and concentrating to 200mL to obtain a konjac polysaccharide extract, then adding 100g of edible gelatin, 10g of bifidobacterium, 10g of lactobacillus acidophilus, 10g of bifidobacterium longum and 50mL of purified water, fully stirring for 10min, and then homogenizing in a homogenizer under the specific conditions as follows: homogenizing at 45 deg.C and 30MPa to obtain microcapsule embedding wall material;

s5, embedding of the compound: homogenizing the block food materials, the coarse cereal mixture and the vegetable and fruit paste stuffing prepared by the steps of S1, S2 and S3, adding 200g of salt, keeping the temperature at 30 ℃ for 20min, and putting 5kg of homogenized stuffing and 1kg of probiotic microcapsule embedding wall material into a microcapsule embedding machine to prepare the 5mm probiotic microcapsule pet food.

Comparative example 4, the parameters were the same as in example 2 except that bifidobacterium was not added in step S1.

Comparative example 5, the same parameters as in example 2 except that Lactobacillus acidophilus was not added in step S1.

Example 3

S1, preparing the blocky food materials: weighing 500g of fresh chicken breast, 500g of duck, 250g of beef, 200g of fresh boned salmon fillet and 65g of fresh chicken heart, cleaning, cutting into blocks of 0.2cm, adding 40mL of purified water, putting into a baking device, and drying at 92 ℃ for 8min for later use;

s2, preparing a coarse cereal mixture: weighing 350g of fresh potatoes, peeling, cutting into blocks, putting 400g of corns, 300g of black rice, 130g of red beans, 130g of eggs, 130g of purple potatoes and 300mL of purified water into a grinder, grinding into mud, filtering out dregs and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, wherein the curing temperature is 95 ℃, and the curing time is 8 min;

s3, preparing vegetable and fruit paste stuffing: weighing 130g of tomatoes, 130g of rapes, 130g of baby cabbages, 130g of carrots, 65g of denucleated apples, 65g of denucleated nectarines and 300mL of purified water, putting the materials into a grinder, stirring the materials into mud, filtering dregs and impurities by using a 10-mesh filter screen, and then putting the mud into a curing machine for curing, wherein the temperature is controlled at 95 ℃ and the curing time is 4 min;

s4, preparation of the probiotic microcapsule embedding wall material: weighing 65g of konjac tubers and 13mL of absolute ethyl alcohol, heating for 10min under the condition that a magnetic stirrer can be heated at a constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000r/min, 5min, taking supernatant, heating and concentrating to 200mL to obtain a konjac polysaccharide extract, then adding 65g of edible gelatin, 6.5g of bifidobacterium, 6.5g of lactobacillus acidophilus, 6.5g of bifidobacterium longum and 38mL of purified water, fully stirring for 6.5min, and then homogenizing in a homogenizer under the specific conditions as follows: the temperature is 35 ℃, the pressure is 20MPa, and the embedding wall material of the microcapsule is obtained after homogenization for standby;

s5, embedding of the compound: homogenizing the blocky food materials, the coarse cereal mixture and the vegetable and fruit paste stuffing prepared by the steps of S1, S2 and S3, adding 130g of salt, keeping the temperature at 23 ℃ for 13min, taking 2kg of homogenized stuffing and 0.7kg of probiotic microcapsule embedding wall material, and putting into a microcapsule embedding machine to prepare the 5mm probiotic microcapsule pet food.

Comparative example 6, the same parameters as in example 3 except that bifidobacterium and lactobacillus acidophilus were not added at step S2.

Comparative example 7, the same parameters as in example 3 except that bifidobacterium and bifidobacterium longum were not added in step S2.

Example 4

S1, preparing the blocky food materials: weighing 800g of fresh chicken breast, 800g of duck, 400g of beef, 250g of fresh boned salmon fillet and 85g of fresh chicken heart, cleaning, cutting into blocks of 0.2cm, adding 45mL of purified water, putting into a baking device, and drying at 96 ℃ for 9min for later use;

s2, preparing a coarse cereal mixture: weighing 450g of fresh potatoes, peeling, cutting into blocks, putting 500g of corns, 400g of black rice, 170g of red beans, 170g of eggs, 170g of purple potatoes and 400mL of purified water into a grinder, grinding into mud, filtering out dregs and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, wherein the curing temperature is 100 ℃, and the curing time is 12 min;

s3, preparing vegetable and fruit paste stuffing: weighing 170g of tomatoes, 170g of rapes, 170g of baby cabbages, 170g of carrots, 90g of denucleated apples, 90g of denucleated nectarines and 400mL of purified water, putting the mixture into a grinder, stirring the mixture into mud, filtering dregs and impurities by using a 10-mesh filter screen, and then putting the mud into a curing machine for curing, wherein the temperature is controlled at 100 ℃, and the curing time is 4.5 min;

s4, preparation of the probiotic microcapsule embedding wall material: weighing 85g of konjac tubers and 17mL of absolute ethanol, heating for 10min under the condition that a magnetic stirrer can be heated at a constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000r/min, 5min, taking supernatant, heating and concentrating to 200mL to obtain a konjac polysaccharide extract, then adding 85g of edible gelatin, 9g of bifidobacterium, 9g of lactobacillus acidophilus, 9g of bifidobacterium longum and 45mL of purified water, fully stirring for 9min, and then homogenizing in a homogenizer under the specific conditions as follows: the temperature is 40 ℃, the pressure is 26MPa, and the embedding wall material of the microcapsule is obtained after homogenization for standby;

s5, embedding of the compound: homogenizing the blocky food materials, the coarse cereal mixture and the vegetable and fruit paste stuffing prepared by the steps of S1, S2 and S3, adding 170g of salt, keeping the temperature at 28 ℃ for 17min, taking 4kg of homogenized stuffing and 0.8kg of probiotic microcapsule embedding wall material, and putting into a microcapsule embedding machine to prepare the 5mm probiotic microcapsule pet food.

Comparative example 8, the same parameters as in example 4 were used except that edible gelatin was changed to pectin at step S4.

The edible gelatin is a hydrolysate of collagen, belongs to a high protein without fat and cholesterol, and is a natural nutritional food thickener. The edible gelatin is used as a powerful protective colloid, has strong emulsifying power, can inhibit the coagulation of proteins such as milk, soybean milk and the like caused by the action of gastric acid after entering the stomach, thereby being beneficial to food digestion, and the edible gelatin is added into the microcapsule embedding wall material to effectively improve the viscosity of the microcapsule and ensure the embedding effect.

Digestibility is the percentage of ingested material that is expressed as a percentage of ingested material, since only a portion of the nutrients that enter the digestive system can be absorbed, and this portion that is absorbed can be measured by digestion testing and used as a parameter to determine digestibility. We can measure the amount of nutrients contained in both feed and faeces and calculate the difference between these two fractions as the fraction ingested by the pet, usually expressed as a percentage or a decimal fraction to 1 (1 indicating complete digestion).

180 Chinese garden dogs, 10kg + -0.5 kg and 2-3 years old, were selected and divided into 12 groups, and there was no statistical difference between the groups, and the compositions prepared in examples 1-4 of the present invention were fed to groups of examples 1-4, and the compositions prepared in comparative examples 1-8 of the present invention were fed to groups of comparative examples 1-8, with a daily feeding amount of 50g/kg, free water collection, and the same feeding conditions: the Chinese gardens were fed in the same environment with the temperature of 20-25 ℃ and the humidity of 50% -60%, and after 3 days of feeding, feces were taken, and the residual amount of protein and the residual amount of fat in the feces were measured, and the results of calculating the digestibility are shown in tables 2 and 3.

TABLE 2

Test sample	Protein content in pet food	Fecal protein content	Digestion and absorption rate
				Example 1	0.85±0.12	0.76±0.14	0.894±0.012
Example 2	0.83±0.16	0.74±0.13	0.891±0.015
				Example 3	0.83±0.12	0.71±0.15	0.855±0.020
Example 4	0.84±0.08	0.71±0.17	0.845±0.019
				Comparative example 1	0.81±0.06	0.64±0.08	0.790±0.018
Comparative example 2	0.80±0.21	0.65±0.02	0.800±0.022
				Comparative example 3	0.81±0.11	0.64±0.06	0.790±0.026
Comparative example 4	0.75±0.15	0.60±0.07	0.800±0.019
				Comparative example 5	0.73±0.21	0.59±0.02	0.808±0.029
Comparative example 6	0.70±0.09	0.58±0.05	0.828±0.027
				Comparative example 7	0.77±0.07	0.61±0.01	0.792±0.016
Comparative example 8	0.78±0.13	0.66±0.01	0.846±0.023

The experimental results in table 2 and fig. 4 show that, compared with examples 2 to 4 and comparative examples 1 to 8, the pet food of example 1 has the highest protein content and the highest digestibility in pet dogs, which indicates that example 1 can provide more nutritious food for pets, and improve the absorption efficiency of pets on food, and reveals that the pet food provided by the present invention is helpful for improving the digestibility of pets.

Digestibility of pet food (in the case of dogs) in short, reflects the ability of the food ingested by dogs to transport essential nutrients, which affects not only the quantity and quality of faeces (how much the dog has drawn, and the character and smell of faeces), but most importantly, the digestibility of pet food affects the long-term health of dogs. Generally, if the digestibility of dog food is 75% or less, then its quality is relatively low; the digestibility is between 75% and 82%, and the product is of medium quality; the digestibility is higher than 82%, and the food is high-quality food.

TABLE 3

Test sample	Fat content in pet food	Fecal fat content	Digestion and absorption rate
				Example 1	0.54±0.003	0.45±0.009	0.833±0.012
Example 2	0.52±0.013	0.43±0.008	0.827±0.015
				Example 3	0.53±0.011	0.39±0.003	0.736±0.020
Example 4	0.51±0.008	0.38±0.002	0.745±0.019
				Comparative example 1	0.48±0.012	0.34±0.007	0.708±0.018
Comparative example 2	0.49±0.009	0.36±0.009	0.734±0.022
				Comparative example 3	0.50±0.007	0.35±0.007	0.700±0.026
Comparative example 4	0.42±0.008	0.31±0.008	0.738±0.019
				Comparative example 5	0.43±0.012	0.30±0.008	0.698±0.029
Comparative example 6	0.40±0.011	0.31±0.007	0.775±0.027
				Comparative example 7	0.48±0.008	0.38±0.006	0.791±0.016
Comparative example 8	0.47±0.007	0.34±0.008	0.723±0.023

The experimental results in table 3 and fig. 4 show that, compared with examples 2 to 4 and comparative examples 1 to 8, the pet food in example 1 has the highest fat content, and the digestibility of the pet dog reaches as high as 83.3%, and the pet food belongs to high-quality pet food, and the data can show that the pet food in example 1 can provide more fat to the pet, improve the gastrointestinal function of the pet, and accelerate the absorption efficiency of the food, so that the pet food provided by the invention is helpful to improve the digestibility of the pet.

As can be seen from tables 2 and 3, the digestibility of protein and fat in the example group is higher than that in the comparative example group, and the digestibility in the comparative example groups 4 to 7 is obviously lower than that in the example group, which indicates that the intestinal absorption efficiency of fat and protein in the Chinese rural dogs is improved due to the synergistic effect among the bifidobacteria, the Lactobacillus acidophilus and the Bifidobacterium longum added in the example group. The reason is that the konjac polysaccharide modified by a special method can provide a higher nutrition level and promote the proliferation of lactobacillus acidophilus and bifidobacterium longum, and the content of probiotics in the intestinal tract of the pet is increased, so that the antiviral rate of the pet cat and dog can be effectively improved, the healthy gastrointestinal wall can be established, and the probability of diseases such as antibiotic-related diarrhea and skin diseases is reduced.

Detection of gastrointestinal push function

30 Kunming white mice were fasted for 20-2 hours and randomly divided into 3 groups, i.e., a control group, example 1 group, and a morpholine group, wherein 10 animals per group were labeled with picric acid. The samples (20.0mg/kg) and the morpholine solution (3.9mg/kg) of the group of example 1 were respectively administered to the drug groups for intragastric administration, the control group was administered with the same amount of physiological saline for intragastric administration, and after 90 minutes, each group was administered with 0.3mL of ink for intragastric administration. After 20 minutes, the cervical vertebrae were sacrificed by dislocation, the abdominal cavity was opened to separate the intestinal membrane, the intestine was cut from the upper end to the pylorus and from the lower end to the ileocecal portion, the small intestine was gently pulled into a straight line on a tray, and the length of the intestine was measured as the total length of the small intestine. The distance from the pylorus to the leading edge of the ink serves as "the ink advancing distance in the intestine". The percent ink advance was calculated using the formula. The ink propulsion rate (%) — the distance (cm) the ink propels in the intestine/the total length (cm) of the small intestine × 100%. The results of the comparison of the intestinal transit function of each group of mice are shown in Table 4.

TABLE 4

As can be seen from the experimental data, the rate of promoting the gastrointestinal peristalsis of the group of example 1 is not as good as that of the group of the morpholine, but the data of the group of example 1 is compared with that of the blank control group, and it can be seen that the pet food prepared by the invention has a certain effect of promoting the gastrointestinal peristalsis. The suspected reason may be that the pet non-ulcer dyspepsia is caused by bad emotions such as tension, anxiety, dysphoria and the like. The pet food prepared by the invention improves the anxiety mood of the pet by mixing the modified konjac polysaccharide and the probiotics, helps the pet to establish a healthy gastrointestinal wall, further plays a role in promoting gastrointestinal peristalsis, and further treats non-ulcer dyspepsia.

The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is specific and detailed, but not construed as limiting the scope of the present invention. It should be understood that any other changes, modifications, substitutions, combinations and simplifications which do not depart from the spirit and principles of the present invention should be construed as equivalents thereof which would be obvious to those skilled in the art, and which are intended to be included within the scope of the present invention.

Claims

1. A composition for improving the intestinal function of dogs and cats is characterized in that: selecting konjac polysaccharide obtained by modifying konjac tubers as a main body of a microcapsule embedding wall material, and adding a plurality of food materials and stuffing matched with balanced nutrition to prepare the composition, wherein the molecular formula of the modified konjac polysaccharide is as follows:

2. a preparation method of a probiotic microcapsule embedding wall material comprises the following steps: weighing 50-100g of konjak tubers and 10-20mL of absolute ethanol, heating for 10-30min under the condition that a magnetic stirrer can be heated and the constant temperature is 60-90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, then adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30-50min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000-: the temperature is 30-45 ℃, the pressure is 10-30MPa, and the embedding wall material of the microcapsule is obtained after homogenization.

3. A preparation method of pet food for improving intestinal functions of dogs and cats is characterized by comprising the following steps: s1, preparing the blocky food materials: weighing 1000g of fresh chicken breast 200-; s2, preparing a coarse cereal mixture: weighing 500g of fresh potato, peeling and cutting into blocks, putting the blocks together with 600g of 300g of corn, 500g of black rice, 200g of red bean, 200g of egg, 200g of purple potato and 500mL of purified water into a grinder for grinding into mud, filtering dregs and impurities by using a 10-mesh filter screen, putting the filtered fluid raw material mud into a curing machine, wherein the curing temperature is 90-105 ℃, and the curing time is 5-15 min; s3, preparing vegetable and fruit paste stuffing: weighing 200g of tomato, 200g of rape, 100g of baby cabbage, 200g of carrot, 100g of stone-removed apple, 50-100g of stone-removed nectarine and 500mL of purified water, putting into a grinder, grinding into mud, filtering out dregs and impurities by using a 10-mesh filter screen, and then putting into a curing machine for curing, wherein the temperature is controlled to be 90-105 ℃, and the curing time is 3-5 min; s4, preparation of the probiotic microcapsule embedding wall material: weighing 50-100g of konjac tubers and 10-20mL of absolute ethanol, heating for 10min under the condition that a magnetic stirrer can be heated at constant temperature of 90 ℃, adding 500mL of 1mol/L hydrochloric acid solution, ultrasonically leaching for 20min, adding 500mL of 1mol/L potassium hydroxide solution, ultrasonically leaching for 30min, and centrifuging by using a centrifuge, wherein the conditions are as follows: 4000r/min, 5min, taking supernatant, heating and concentrating to 200mL to obtain a konjac polysaccharide extract, then adding 50-100g of edible gelatin, 5-10g of bifidobacterium, 5-10g of lactobacillus acidophilus, 5-10g of bifidobacterium longum and 30-50mL of purified water, fully stirring for 5-10min, and then homogenizing in a homogenizer under the specific conditions as follows: homogenizing at 30-45 deg.C and 10-30MPa to obtain microcapsule embedding wall material; s5, embedding of the compound: homogenizing the blocky food materials, the coarse cereal mixture and the vegetable and fruit paste stuffing prepared by the steps of S1, S2 and S3, adding 100-200g of salt, keeping the temperature at 20-30 ℃ for 10-20min, taking 1-5kg of homogenized stuffing and 0.5-1kg of probiotic microcapsule embedding wall material, and putting the obtained materials into a microcapsule embedding machine to obtain the 5mm probiotic microcapsule pet food.

4. The preparation method of the pet food for improving the intestinal function of dogs and cats according to claim 3, wherein the preparation method comprises the following steps: 200g of fresh chicken breast, 200g of duck, 150g of beef, 150g of fresh boned salmon fillet, 50g of fresh chicken heart and 30mL of purified water are weighed in the S1.

5. The method for preparing pet food for improving intestinal function of dogs and cats according to claim 4, wherein the method comprises the following steps: 300g of fresh potatoes, 300g of corns, 200g of black rice, 100g of red beans, 100g of eggs, 100g of purple potatoes and 200mL of purified water are weighed in the S2.

6. The preparation method of the pet food for improving the intestinal function of dogs and cats according to claim 5, wherein the preparation method comprises the following steps: 100g of tomatoes, 100g of rapes, 100g of baby cabbage, 100g of carrots, 50g of denucleated apples, 50g of denucleated nectarines and 200mL of purified water are weighed in the S3.

7. The preparation method of the pet food for improving the intestinal function of dogs and cats according to claim 6, wherein the preparation method comprises the following steps: 50g of konjac tubers, 10mL of absolute ethyl alcohol, 500mL of 1mol/L hydrochloric acid solution, 500mL of 1mol/L potassium hydroxide solution, 50g of edible gelatin, 5g of bifidobacteria, 5g of lactobacillus acidophilus, 5g of bifidobacterium longum and 30mL of purified water are weighed in the S4.

8. The preparation method of the pet food for improving the intestinal function of dogs and cats according to claim 7, wherein the preparation method comprises the following steps: the amount of the salt weighed in the S5 is 100g, the amount of the stuffing is 1kg, and the amount of the embedding wall material of the probiotic microcapsule is 0.5 kg.

9. Composition for improving the intestinal function of dogs and cats, pet food prepared by the preparation method according to any one of claims 1-8.