CN114939135B

CN114939135B - Application of traditional Chinese medicine composition in preparation of medicines for treating cough and asthma caused by lung heat of pigs

Info

Publication number: CN114939135B
Application number: CN202210678351.2A
Authority: CN
Inventors: 兰新财; 缪雨臻; 杨彦华; 赵丽丽; 安丽娜; 缪雪荣
Original assignee: Zhejiang Dkcom Animal Health Products Co ltd
Current assignee: Zhejiang Dkcom Animal Health Products Co ltd
Priority date: 2022-05-11
Filing date: 2022-05-11
Publication date: 2023-08-22
Anticipated expiration: 2042-05-11
Also published as: CN114939135A

Abstract

The invention relates to an application of a traditional Chinese medicine composition in preparing medicines for treating cough and asthma caused by lung heat of pigs, belonging to the application field of the traditional Chinese medicine composition in treating respiratory diseases of pigs. The composition comprises 250-500 parts of bistort rhizome, 100-350 parts of myrobalan, 100-200 parts of giant knotweed, and 250-350 parts of dyers woad leaf by weight. The traditional Chinese medicine composition is prepared into a concentration of 2g/L in the form of traditional Chinese medicine granules according to a dosage of 0.12g/kg of pig weight, and is added into drinking water for free drinking and continuously used for 5 days. The traditional Chinese medicine composition can effectively treat cough and asthma caused by lung heat of pigs, has a cure rate of 82%, has a total effective rate of 98%, is basically equivalent to 78% and 94% of the total effective rate of the Yinhuang oral liquid group, and has the advantages that symptoms are relieved or disappeared quickly in the process of administration, and recovery conditions such as body temperature, respiratory rate, symptom meter and the like are obvious or extremely obvious better than those of the Yinhuang oral liquid group.

Description

Application of traditional Chinese medicine composition in preparation of medicines for treating cough and asthma caused by lung heat of pigs

Technical Field

The invention relates to a traditional Chinese medicine composition and application of the composition in treating cough and asthma caused by lung heat of pigs. Belongs to the technical field of the prescription of traditional Chinese medicine and the application thereof in animal respiratory diseases.

Background

Today, the pig industry develops towards a centralized, intensive, specialized and industrial modern pig raising production system, and epidemic diseases are complex and difficult to effectively control continuously. According to pig epidemic disease investigation, the diseases of the respiratory tract and the digestive tract are mainly high in pig farms, and especially the diseases of the respiratory tract are most common, so that the health of pig groups is seriously endangered, and great economic loss is caused for pig industry. The main symptoms of the pig respiratory disease are lung heat cough and asthma.

According to the records of the encyclopedia of agricultural encyclopedia of China, chinese veterinarian volume, lung heat and lung obstruction, heat evil obstructing the lung. The light one is lung heat and the heavy one is lung congestion. It is usually caused by pathogenic heat entering the mouth and nose, or wind-cold and wind-heat entering the interior to transform heat, and heat accumulating in the lung. It is usually found in lung heat and lung abscess. The syndrome: thirst, heat in ears and nose, sweating, shortness of breath, dyspnea, cough, heavy and light day and night, thick and yellow nasal discharge, chest pain, dry feces, scanty and red urine, red and yellow and thick mouth, and rapid or heavy pulse. Syndrome analysis: this syndrome is characterized by the common symptoms of lung disease and by the syndrome of internal heat. Excessive heat obstructing the lung, which is caused by excessive heat and pathogenic qi entering the body, can strengthen the heat and quench the thirst; the heat forces body fluid to leak out and sweat, and the body fluid is decocted to form a yellow nasal discharge; cough due to upward flow of lung qi; the lung is not clear and the asthma is rough and dyspnea is caused; chest pain may occur if the heat damages the lung collaterals and collaterals are not smooth with qi and blood; excessive internal heat, loss of body fluids, loss of moistening of the intestines, constipation due to dry feces, and scanty and reddish urine. A red tongue with yellow coating and a rapid and rapid pulse or a deep and rapid pulse are all symptoms of internal heat.

The clinical symptoms and phenomena are mainly cough and asthma caused by internal lung stagnated heat and lung qi failure and due to various reasons, and are chronic respiratory infectious diseases of pigs. Dyspnea, cough and asthma are the main characteristics. The mycoplasma hyopneumoniae has philia to the lung, and mainly invades the lung of pigs, so that the respiratory function of the lesion part of the lung is lost. Its main lesions are the lungs, the lymph nodes of the hilum and the mediastinum. The anterior lower parts of the tip leaf, the heart leaf, the middle leaf and the diaphragm leaf of the lung form a light red or gray red which is bilaterally symmetrical, is semitransparent, has obvious limit and is similar to fresh and tender muscle-like lesions, and is commonly called as 'meat change'. As the disease is aggravated, the color and luster of the lesions become deep, the toughness is increased, the appearance is opaque, and the lesions are commonly called pancreas change or shrimp meat change. The hilum and mediastinum lymph nodes are significantly enlarged. The healthy growth of pigs is seriously jeopardized, the slaughtering percentage of live pigs is reduced, and great economic loss is caused for pig industry.

The main prevention and treatment means of cough and asthma caused by lung heat of pigs are mainly vaccine immunity and antibiotic treatment. Immunosuppression is prevalent due to the complex disease, frequent immunity. In addition, the pathogenic serotypes are more, the cross protection capability is poor, and vaccine immunity cannot cover all diseases. In respiratory diseases, common antibiotics such as tilmicosin, tiamulin, florfenicol, doxycycline and the like are applied. However, excessive use of vaccines and chemical drugs, frequent injection of vaccines, large stress response, and severe cases can lead to immune paralysis, resulting in immune failure. Excessive use of chemical drugs, especially antibiotics, has reduced immunity, increased burden on liver and kidney, and drug resistance, and severe double infection. The result is that we see that the more vaccine is, the more medicine is used, and the dosage is continuously improved, but the swine disease is not controlled, but is more and more complicated and difficult to cure. So the state at present brings the non-antibiotic Chinese medicine compound preparation to the schedule and encourages the development and research of the Chinese medicine compound preparation.

According to clinical symptoms and pathological changes, the syndrome differentiation of the traditional Chinese medicine expert is considered to belong to the category of lung heat cough and asthma syndrome of a middle veterinarian, and the traditional Chinese medicine has the advantages of remarkable curative effect on treating lung heat cough and asthma, small toxic and side effects, no drug resistance, cross infection and the like. So, the treatment prescription of cough and asthma syndrome is recorded in the book of pig Jingdan, namely, the treatment method of cough and asthma syndrome of pigs: cortex Dictamni Radicis, hepar Sus Domestica and pulmonis Domestica tip are decocted together for feeding. And two eggs are stewed and fed. The following steps: three insect threads, entering eggs to burn cooked food, etc. However, the traditional mode of decoction administration of traditional Chinese medicines is not suitable for the current large-scale cultivation situation. In response to the call of developing antibiotic-free traditional Chinese medicines in China, a traditional Chinese medicine composition (also called Shenqing Qingshen cough-relieving granules) is invented, pharmacodynamics, toxicology, pharmacy and clinical tests are completed at present, and a new certificate of agricultural department of China is to be declared. The special treatment effect of the traditional Chinese medicine composition without antibiotics and addressing both symptoms and root causes improves the recovery rate, survival rate and slaughter rate of pigs so as to adapt to the development of a centralized, intensive, specialized and industrial modern pig raising production system.

Disclosure of Invention

A traditional Chinese medicine composition and application thereof in treating cough and asthma caused by lung heat of pigs belong to the field of traditional Chinese medicine formulas and application thereof in animal respiratory diseases. The composition comprises 250-500 parts of bistort rhizome, 100-350 parts of myrobalan, 100-200 parts of giant knotweed, and 250-350 parts of dyers woad leaf by weight. The traditional Chinese medicine composition is prepared into 2g/L solution by water in the form of traditional Chinese medicine granules, and is taken by pigs in free drinking water for 5 days. The traditional Chinese medicine composition can effectively treat cough and asthma caused by lung heat of pigs, has a cure rate of 82%, has a total effective rate of 98%, is basically equivalent to 78% and 94% of the total effective rate of the Yinhuang oral liquid group, and has the advantages that symptoms are relieved or disappeared quickly in the process of administration, and recovery conditions such as body temperature, respiratory rate, symptom meter and the like are obvious or extremely obvious better than those of the Yinhuang oral liquid group.

The invention solves the technical problems by adopting the following scheme:

the 4 traditional Chinese medicines composing the preparation are recorded in Chinese veterinarian pharmaceutical books, and have the effects of clearing heat and detoxicating, relieving cough and reducing sputum and relieving asthma, and detumescence and relieving sore throat, and are mainly used for treating lung heat cough and asthma, sore throat and other diseases. The bistort rhizome has bitter and astringent taste and slightly cold nature, enters lung, large intestine and liver channels, has the effects of clearing heat and detoxicating, detumescence and hemostasis, and is mainly used for treating lung heat cough, blood heat swelling and toxin, red dysentery and heat diarrhea and the like. Meanwhile, bistort rhizome is also a common medicine for Mongolian medicine, has the effects of clearing lung heat and relieving swelling, is used for treating lung heat, pestilence and the like, and is a monarch drug; the dyers woad leaf has bitter and cold properties, returns to heart and stomach channels, has the effects of clearing heat and detoxicating, cooling blood and removing spots, is mainly used for treating the symptoms of hot disease spots, sore throat, heat dysentery, jaundice, carbuncle swelling, erysipelas and the like, strengthens the effects of clearing heat and detoxicating, detumescence and relieving sore throat of the monarch drug, and is a ministerial drug; giant knotweed rhizome has slightly bitter taste and slightly cold nature, enters lung, liver and gall meridians, has the effects of relieving cough and reducing phlegm, clearing heat and detoxicating, activating blood and relieving pain and the like, and is mainly used as an adjuvant drug for treating cough due to lung heat, jaundice due to damp-heat, carbuncles, swelling and sore toxin and the like; the myrobalan has bitter, sour and astringent taste, is flat in nature, enters lung and large intestine meridians, has the effects of astringing lung to relieve cough, relieving sore throat to open voice, astringing intestine to check diarrhea, and is mainly used for treating lung deficiency cough and asthma, chronic diarrhea and chronic dysentery and the like, and is used together with polygonum cuspidatum as an adjuvant drug. Chebulae fructus also plays a role in guiding drugs into the lung and harmonizing the roles of various drugs, and is also called as guiding drugs and guiding drugs in the recipe.

In summary, although the myrobalan is one medicinal material, the three effects of the medicine composition are exerted, namely, the medicine composition has the effects of three medicinal materials, and plays an extremely important role in clinical curative effect. The concrete steps are as follows: first, chebulae fruit, fructus Chebulae, with its nature and flavor, enters meridians and functions mainly, and together with Polygonum cuspidatum as an adjuvant drug. Secondly, the chebula is the most commonly used Tibetan medicine, and in the classical works of Tibetan medicine, "Jingzhu Ben Cao," the chebula is called "the king of Tibetan medicine", or one of the traditional Chinese medicines which are introduced into the lung and are carried upward and introduced into the lung are used as the menstruation guiding medicines. Third, chebulae fructus has the effect of harmonizing various drugs, so it also plays the role of guiding drugs in the prescription.

In conclusion, bistort rhizome has the effects of clearing heat and detoxicating and clearing lung heat, and is a monarch; the dyers woad leaf has the effects of clearing heat and detoxicating, and helps bistort rhizome to clear heat and detoxify, and is minister; the giant knotweed rhizome has the effects of relieving cough, resolving phlegm, clearing heat and detoxicating, on one hand, the assistant and monarch drug has the effects of clearing lung heat and relieving cough, and on the other hand, the assistant and ministerial drug has the effects of clearing heat and detoxicating, and is an adjuvant drug; the myrobalan has the effects of astringing lung and relieving cough, and is coordinated with the monarch drug bistort rhizome, one ascending and one descending, one breathing and one opening and one closing, and the balance, so that the medicine has ingenious and proper compatibility, is used as an adjuvant drug together with the giant knotweed, has the effects of carrying medicine upwards, guiding the medicine into the lung and harmonizing the medicines, and plays roles of guiding the medicine and guiding the medicine. The medicines are combined together to play roles of clearing heat and detoxicating, resolving phlegm and relieving cough and asthma.

The traditional Chinese medicine composition consists of bistort rhizome, myrobalan, giant knotweed rhizome and dyers woad leaf, and comprises, by weight, 250-500 parts of bistort rhizome, 100-350 parts of myrobalan, 100-200 parts of giant knotweed rhizome and 250-350 parts of dyers woad leaf.

The traditional Chinese medicine composition is preferably 500 parts of bistort rhizome, 200 parts of myrobalan, 100 parts of giant knotweed rhizome and 200 parts of dyers woad leaf according to parts by weight.

The traditional Chinese medicine composition also preferably comprises 450 parts of bistort rhizome, 150 parts of myrobalan, 150 parts of giant knotweed and 250 parts of dyers woad leaf according to parts by weight.

The traditional Chinese medicine composition also preferably comprises 400 parts of bistort rhizome, 200 parts of myrobalan, 100 parts of giant knotweed and 300 parts of dyers woad leaf according to parts by weight.

The traditional Chinese medicine composition also preferably comprises 300 parts of bistort rhizome, 300 parts of myrobalan, 120 parts of giant knotweed and 280 parts of dyers woad leaf according to parts by weight.

The traditional Chinese medicine composition also preferably comprises 250 parts of bistort rhizome, 300 parts of myrobalan, 100 parts of giant knotweed rhizome and 350 parts of dyers woad leaf according to parts by weight.

The traditional Chinese medicine composition is powder, granules and oral liquid.

The preparation process of the powder comprises the following steps: taking bistort rhizome, myrobalan, giant knotweed rhizome and dyers woad leaf according to the weight part ratio of the composition, crushing, sieving with a 80-mesh sieve, uniformly mixing, and sub-packaging into 1000g of powder with the prescription dosage, thus obtaining the ginseng green pestilence clearing and cough relieving powder.

The preparation process of the granule comprises the following steps: taking bistort rhizome, myrobalan and giant knotweed rhizome according to the weight part ratio of the composition, crushing the bistort rhizome, the myrobalan and the giant knotweed rhizome into blocks with the size of 0.5 cm-0.8 cm, mixing the blocks with dyers woad leaf, adding 70% ethanol, carrying out reflux extraction for 2 times, each time for 2-2.5 hours, filtering, recovering ethanol from filtrate, and obtaining clear paste for later use; adding 6 times of water into the residues, decocting for 1-1.5 hours, filtering, concentrating the filtrate under reduced pressure to obtain fluid extract with the relative density of 1.05 (60 ℃), mixing with the ethanol extract, and continuously concentrating under reduced pressure to obtain fluid extract with the relative density of 1.10 (60 ℃), for later use; mixing dextrin 260g and sucrose 480-520 g, spraying and granulating with the prepared fluid extract as adhesive, and preparing 1000g of granule according to a prescription, which is also called SHENQINGQINGWEN cough relieving granule.

The preparation process of the oral liquid comprises the following steps: taking bistort rhizome, myrobalan and giant knotweed rhizome according to the weight part ratio of the composition, crushing the bistort rhizome, the myrobalan and the giant knotweed rhizome into blocks with the size of 0.5 cm-0.8 cm, mixing the blocks with dyers woad leaf, adding 70% ethanol, carrying out reflux extraction for 2 times, each time for 2-2.5 hours, filtering, recovering ethanol from filtrate, and obtaining clear paste for later use; adding 6 times of water into the residues, decocting for 1-1.5 hours, filtering, concentrating the filtrate under reduced pressure to obtain clear paste with the relative density of 1.05 (60 ℃), mixing with the alcohol extract, uniformly mixing, refrigerating and standing for 24 hours, filtering, diluting the filtrate with distilled water to 1g of raw medicinal material per 1ml, filling, and sterilizing to obtain the ginseng green pestilence-clearing cough-relieving oral liquid.

The above 3 dosage forms of the traditional Chinese medicine composition have the effects of clearing heat and detoxicating, resolving phlegm and relieving cough and asthma. Has good treatment effect when being used for treating cough and asthma caused by lung heat of pigs. The ginseng green fever-relieving cough-relieving granule is prepared by comparing multiple indexes, preferably a prescription of 400 parts of bistort rhizome, 200 parts of myrobalan, 100 parts of giant knotweed rhizome and 300 parts of dyers woad leaf, and the drug effect, toxicology and clinical test are carried out:

pharmacodynamic test

The Shenqing granule for clearing plague and relieving cough has the effects of clearing heat and detoxicating, resolving phlegm and relieving cough and asthma. Is mainly used for treating cough and asthma caused by lung heat of pigs. The tests of anti-inflammatory, antipyretic, in vitro anti-endotoxin, and inhibiting rabbit diffuse intravascular coagulation caused by endotoxin, regulating the functions of mouse spleen lymphocytes, inhibiting PRRSV infection of Marc-145 cells, relieving cough, reducing sputum and relieving asthma are designed, and the results are as follows:

1. in order to observe the anti-inflammatory effect of the Shenqing Qingshen cough-relieving granules, pouring a stomach Shenqing cough-relieving granule solution into a mouse for 3 continuous days, then using dimethylbenzene to cause inflammation of the right auricle of the mouse, killing the mouse after 4 hours, cutting off two ears along the baseline of the auricle, using a 6mm puncher to punch round ear pieces on the same part of the left ear and the right ear, weighing, and calculating the swelling rate; rats were continuously perfused with the granule solution for 3 days, the volume of the right hind toe of the rat was measured, 1% carrageenan was used to cause inflammation, the volume was measured after 3 hours and the swelling degree was calculated. As a result, the Shenqingqing pestilence relieving cough granule with dosages of 0.94g/kg body weight, 0.47g/kg body weight and 0.235g/kg body weight is taken orally by the mice, and has obvious inhibition effect on auricle swelling of the mice caused by dimethylbenzene; oral administration of the Shenqing qing pestilence-clearing cough-relieving granules with the dosage of 0.64g/kg body weight, 0.32g/kg body weight and 0.16g/kg body weight to rats has obvious inhibition effect on rat toe swelling caused by carrageenan, and shows that the Shenqing qing pestilence-clearing cough-relieving granules have better anti-inflammatory effect.

2. In order to examine the heat-clearing and detoxicating effects of the Shenqing Qingshen cough-relieving granules, a rabbit which is not injected with a pyrogen is selected, the Shenqing cough-relieving granules with high, medium and low doses are infused continuously for 3 days, and then the escherichia coli endotoxin is injected intravenously at the edge of the ear to measure the body temperature and observe the body temperature change of the rabbit. Selecting rats which are not injected with the pyrogen, subcutaneously injecting the yeast suspension to generate the heat, and establishing a rat heating model. And then high, medium and low doses of Shenqing qing pestilence relieving cough granules are infused, the body temperature is measured, and the influence of the Shenqing qing pestilence relieving cough granules on the body temperature of a model rat is observed. The result shows that intravenous injection of the escherichia coli endotoxin at the edge of rabbit ear at the dosage of 0.6 mug/kg body weight can continuously and stably raise the body temperature of the rabbit, and successfully causes a rabbit fever model; oral administration of granule containing Ginseng radix and radix Isatidis for relieving fever and cough at doses of 168mg/kg body weight and 336mg/kg body weight can effectively reduce endotoxin-induced fever of rabbit body temperature. The body temperature of the rat can be continuously and stably increased by subcutaneously injecting the yeast suspension with the weight of 1.5g/kg into the rat, so that a rat fever model is successfully caused; the oral granule for clearing and relieving fever and cough with dosage of 0.32g/kg body weight and 0.64g/kg body weight can effectively reduce the body temperature of the rats heated by the yeast, and the results show that the granule for clearing and relieving fever and cough with medium and high dosage has better heat-removing effect.

3. In order to examine the related effect of the Shenqing qingwenzhike granule on resisting endotoxin, a limulus reagent method is adopted to observe the in vitro anti-endotoxin effect, and a blood viscosity test, a plasma protamine side coagulation test and a calcium recovery test are adopted to observe the effect of the Shenqing qingwenzhike granule on inhibiting the diffuse intravascular coagulation of rabbits caused by endotoxin. The result shows that 0.25mg/ml of the Shenqingqingqingwenzhike granule has the effect of resisting endotoxin in vitro. 168mg/kg of Shenqing plague relieving cough granule is taken orally to the rabbit, so that the plasma protamine side coagulation test of the endotoxin dispersion intravascular coagulation rabbit is changed from positive to negative, the plasma calcium recovery time is shortened, and the plasma viscosity is reduced; the dosage of the Shenqing Qingshen cough relieving granule has antagonism on the dispersive intravascular coagulation of the endotoxin rabbit.

4. In order to examine the regulation effect of the Shenqing pestilence relieving cough granule on the functions of the mouse spleen lymphocytes, the test adopts an MTT method to carry out ConA and LPS induced mouse spleen lymphocyte proliferation test, and an ELISA method is used for detecting the contents of cytokines IL-2 and IFN-gamma in the supernatant of the ConA stimulated mouse spleen lymphocytes and the contents of cytokines TNF-alpha, IL-4 and IL-6 in the supernatant of the LPS stimulated mouse spleen lymphocytes. The results show that the Shenqingqing pestilence relieving cough granules with the dosage of 0.25mg/ml and 0.5mg/ml can promote the proliferation of mouse spleen lymphocytes induced by ConA or LPS; the Shenqingqingqingwenzhike granule with the dosage of 0.25mg/ml and 0.5mg/ml has the promotion effect on the secretion of IL-2 by the spleen lymphocytes of the mice stimulated by ConA; the Shenqing Qingshen cough relieving granule can obviously promote IFN-gamma secretion by the spleen lymphocytes of the mice stimulated by ConA. The granule for relieving cough and clearing plague with Ginseng radix green at the dosage of 0.125mg/ml, 0.25mg/ml and 0.5mg/ml can obviously inhibit the secretion of TNF-alpha by the spleen lymphocytes of mice stimulated by LPS; the Shenqingqing pestilence relieving cough granule with the dosage of 0.25mg/ml and 0.5mg/ml can obviously inhibit the secretion of IL-4 by the spleen lymphocytes of mice stimulated by LPS; the Shenqingqing pestilence relieving cough granule with the dosage of 0.25mg/ml and 0.5mg/ml can obviously inhibit the secretion of IL-6 by the spleen lymphocytes of mice stimulated by LPS. Therefore, the Shenqing granule for clearing plague and relieving cough has a certain promotion effect on the cellular immunity of the organism and can inhibit the inflammatory reaction of the organism to a certain extent.

5. In order to examine the in vitro antiviral effect of the Shenqing Qingshen cough relieving granule, the experiment adopts a cell culture method, and observes the influence of the Shenqing cough relieving granule on Marc-145 cells infected by porcine reproductive and respiratory syndrome virus (PRRSV CH-1R strain). The test first examined the toxicity of Shenqing Qingshen cough-relieving granule to Marc-145 cells and the virulence of porcine reproductive and respiratory syndrome virus (PRRSV CH-1R strain), then the test was carried out with a drug containing 100TCID ₅₀ Marc-145 cells were incubated with a viral load of culture for 2h, followed by 60h with a culture containing Shenqing Qingshen cough-relieving particles, cytopathic effects were observed, and differences between groups were detected by MTT method. The results show that the Shenqing Qingshen cough relieving particles with the concentration of 3 of 0.5mg/ml, 0.25mg/ml, 0.125mg/ml and the like have no toxicity to Marc-145 cells; the Shenqingqingqing pestilence cough relieving granule with the concentration of 0.5mg/ml and 0.25mg/ml and 2 times can inhibit PRRSV (CH-1R strain) from infecting Marc-145 cells, and lighten Marc-145 cytopathy caused by the strain.

6. In order to observe the cough relieving effect of the Shenqing qing pestilence cough relieving granules, 50 Kunming mice are selected and randomly divided into 5 groups, 10 mice in each group and each half of female and male mice in each group are respectively a negative control group (normal saline), a positive medicine control group (2.275 ml/kg body weight of the Zhenqian honey-refined fritillary and loquat leaf extract), a high-dose group (0.94 g/kg body weight) of Shenqing qing pestilence cough relieving granules, a medium-dose group (0.47 g/kg body weight) of Shenqing qing pestilence cough relieving granules and a low-dose group (0.235 g/kg body weight) of Shenqing qing pestilence cough relieving granules, the mice in the negative control group are respectively filled with 0.5ml/20g body weight of normal saline per day, the positive medicine control group are respectively filled with 0.5ml/20g body weight of the Zhenqian honey-refined fritillary and loquat leaf extract diluent with the corresponding concentrations of Shenqing qing pestilence cough relieving granules per day, and the mice in the medium-low-dose groups are respectively filled with the corresponding concentrations of Shenqing qing pestilence cough relieving granules per day, and the body weight is continuously 3d. After the last administration for 1h, the mice are placed in a self-made closed container, ultrasonic atomization is performed, 25% ammonia water is sprayed for 20s, and timing is started, so that the abdominal muscles of the mice contract or contract the chest, and simultaneously, the mouth is opened, and sometimes cough sound is generated, and the cough is counted as 1 time. The time to first cough and the number of cough in 2min were recorded and the cough suppression rate was calculated relative to the control group. As a result, the oral administration of the Shenqing qing pestilence cough relieving granules with the weight of 0.94g/kg and the weight of 0.47g/kg to mice has obvious inhibition effect on cough caused by ultrasonic atomization ammonia water stimulation, the first cough time is delayed to 31.0s and 30.1s from 15.3s of a negative control group, the number of times of 2min cough is reduced to 19.1 and 19.7 from 32.1 of the negative control group, and the cough inhibition rate reaches 40.5 percent and 38.6 percent respectively, so that the Shenqing qing pestilence cough relieving granules with the dosage has certain cough relieving efficacy.

7. In order to examine the phlegm eliminating effect of the Shenqing pestilence relieving granules, 50 Kunming mice are selected and randomly divided into 5 groups, each group is 10, and each male and female is half, wherein the groups are respectively a negative control group (normal saline), a positive drug control group (ambroxol hydrochloride 10mg/kg body weight), a Shenqing pestilence relieving granule low dose group (0.235 g/kg body weight), a Shenqing pestilence relieving granule medium dose group (0.47 g/kg body weight) and a Shenqing pestilence relieving granule high dose group (0.94 g/kg body weight), the mice in the negative control group are filled with normal saline 0.5ml/20g body weight each day, the mice in the positive drug control group are filled with ambroxol hydrochloride suspension 0.5ml/20g body weight, and the mice in the Shenqing pestilence relieving granules in the high, medium and low dose groups are filled with the Shenqing pestilence relieving granule diluent of the corresponding concentration each day for 0.5ml/20g body weight, and the continuous 3d. The injection of 5% phenol red physiological saline solution is 0.5 ml/unit in the abdominal cavity after the last administration for 1h, and the cervical dislocation is killed after 30min of injection. Fixing the mouse on foam plate, cutting off skin in the middle of neck after blood is slightly coagulated, separating trachea, cutting off a section of trachea from thyroid cartilage to trachea branch, placing into a container containing 2ml of 5% NaHCO ₃ In a test tube of the solution, standing for 1h, taking 100 μl of the supernatant, measuring and recording OD value at 558nm of an enzyme label instrument, calculating phenol red content (μg/ml) according to a phenol red standard curve, and calculating tracheal phenol red excretion promotion rate. The results prove that the oral administration of the high and medium dose Shenqing pestilence-clearing cough-relieving granules by mice can obviously increase the discharge amount of the tracheal phenol red, compared with 0.89 mug/ml of a negative control group, the discharge amount of the tracheal phenol red is respectively increased to 1.13 mug/ml and 1.12 mug/ml, the discharge promotion rate of the tracheal phenol red reaches 27.02 percent and 25.63 percent, and the effect of the oral administration of the high and medium dose Shenqing pestilence-clearing cough-relieving granules is equivalent to that of a positive control drug ambroxol hydrochloride, so that the Shenqing pestilence-clearing cough-relieving granules have a certain phlegm-eliminating effect.

8. In order to observe the asthma relieving effect of the Shenqing granule for clearing plague and relieving cough, a medicine asthma guiding method is adopted for test. The guinea pigs are randomly divided into 5 groups, and 6 groups are respectively a positive control group, a positive medicine control group, a Shenqing antipyretic cough-relieving granule high-dose group, a Shenqing antipyretic cough-relieving granule medium-dose group and a Shenqing antipyretic cough-relieving granule low-dose group. Normal feeding of a positive control group; the Shenqing qing pestilence relieving cough granule test group is fed by a mixed feeding method, and the dosage is respectively 0.64g/100g of feed, 0.32g/100g of feed and 0.16g/100g of feed, and the feed is continuously fed for 3 days; the positive drug control group guinea pigs were fed aminophylline on day 3 in feed at a dose of 1 tablet per 550g feed. After 3 days, each group of guinea pigs is placed in a self-made closed container, histamine phosphate of 2mg/ml is sprayed into the container by ultrasonic atomization for 20s, the asthma inducing latency period of each guinea pig and the number of falling animals within 6min are observed, and the asthma inducing latency period exceeds 6min and is counted by 360 s. The results show that the oral administration of the ginseng green pestilence-clearing and cough-relieving granules with the dosage of 0.64g/100g feed, 0.32g/100g feed and 0.16g/100g feed by guinea pigs can effectively relieve the asthma of the guinea pigs caused by the histamine phosphate, greatly prolong the asthma-inducing incubation period of the histamine phosphate, and respectively reach 5.42 times, 5.46 times and 4.04 times of the asthma-relieving inhibition rate of the groups with high, medium and low dosages compared with the negative control group, so that the ginseng green pestilence-clearing and cough-relieving granules with the dosage have better asthma-relieving effect.

2. Pharmacological toxicity test

1. Acute toxicity test study in order to evaluate the safety of Shenqing Qingshen cough relieving granules, an acute toxicity test was performed on the same. The test uses 2 experimental animals, namely rats and mice, and the toxicity of the experimental animals to the oral administration of the test animals is observed. Dissolving SHENQINGQINGWEN cough relieving granule with distilled water, and administering to rats and mice with 5000mg/kg body weight to treat acute toxicity test LD of SHENQINGWEN cough relieving granule to rats and mice ₅₀ More than 5000mg/kg body weight, the preparation is proved to be practically nontoxic, and the preparation has higher safety.

2. In order to examine the sub-chronic toxic effect of the Shenqing qingwen cough granules, 80 Wistar rats are randomly divided into 4 groups, namely a male-female cage, a high-dose group, a medium-dose group, a low-dose group and a control group of the Shenqing qingwen cough granules, 20 of the groups are respectively divided into halves of each male and female group. Dissolving the ginseng green pestilence-eliminating cough-relieving particles with distilled water, and diluting to a concentration of 1.2g/ml, 0.6g/ml and 0.12 g/ml. The dosage of the Shenqing Qingshen cough relieving granule is respectively 12g/kg body weight, 6g/kg body weight and 1.2g/kg body weight, and 1ml/100g body weight is administrated by 1 time of stomach infusion. The control group was filled with an equal volume of physiological saline every day. The administration was continued for 4 weeks. The main observation indexes include general health condition, weight, feed intake, water intake, hematology and serum biochemical indexes, viscera coefficients, pathological section changes, pathological histology observation and the like. As a result, after taking Shenqingqing pestilence relieving cough granules, the rats in the groups with high, medium and low doses have no morbidity or mortality, and the spirit, behavior, diet, urination and weight of the rats are not abnormal. Further deeply detecting that abnormal changes do not occur in blood routine, serum biochemical indexes, organ coefficients and important organ tissue morphology observation of the tested animals, and indicating that the Shenqingqing plague relieving cough granule has higher clinical medication safety.

3. Clinical trial data

1. Safety test for target animals

In order to determine the medication safety of the Shenqing Qingshen cough relieving granules on pigs, 40 white pigs of No. 1, which are weaned army 1 with the age of 35 days and have similar weight, are randomly divided into a control group, a 1-time dose group, a 3-time dose group and a 5-time dose group, and 10 pigs of each group are subjected to clinical safety tests. The method comprises the following steps: the Shenqing Qingshen cough relieving granule is orally taken to 35-day-old healthy piglets at the dosage of 0.12g, 0.36g and 0.60g per 1kg body weight, which is equivalent to 1 time, 3 times and 5 times of recommended dosages, and is continuously taken for 5 days, and the clinical manifestations, feed intake and body weight changes, hematological and serum biochemical indexes, the anatomical and histological changes of the tested pigs are observed in detail after the administration. Results: the Shenqingqing pestilence cough granules at 1 times, 3 times and 5 times of the clinically recommended reference dose do not cause death and other toxic reactions of the test pigs. The test pigs have no abnormal mental state, eating desire and feces, good growth and development, and no obvious difference between red blood cell number, hemoglobin content, total white blood cell number, white blood cell sorting count and various serum biochemical indexes compared with the control group. The tissue structure and morphology of each organ are not observed any abnormal lesions by the section examination and histological observation. Conclusion: the Shenqing granule for clearing plague and relieving cough has higher clinical medication safety for pigs.

2. Clinical drug dosage screening

Because the modeling is unsuccessful, the pre-experiment adopts natural disease cases to carry out the Shenqingqing qing pestilence cough granule dosage screening test.

30 sick pigs and 6 healthy pigs with typical clinical symptoms of swine lung heat cough and asthma are collected from a sick pig farm for testing, and the clinical symptoms and the test indexes of the sick pigs meet inclusion standards. Every 6 groups, wherein the different dose groups are 5 groups and the healthy control group is 1 group.

An effective test and a dose screening test of treating the clinical natural onset swine lung heat cough and asthma by different doses of the Shenqing qing pestilence relieving cough granules are carried out to search the effective dose range of the Shenqing qing pestilence relieving cough granules for treating the swine lung heat cough and asthma. The method comprises the following steps: the traditional Chinese medicine preparation Shenqing Qingshen cough granule is infused into pigs with cough and asthma caused by lung heat with natural incidence at dosages of 0.08g/kg body weight, 0.12g/kg body weight, 0.16g/kg body weight, 0.20g/kg body weight and 0.24g/kg body weight, and is continuously taken for 5 days for 1 time every day. Results: within 5 days of administration, 0.12g/kg body weight, 0.16g/kg body weight, 0.20g/kg body weight and 0.24g/kg body weight can achieve ideal treatment effects, and the body temperature, cough frequency, respiratory rate and symptom score of each group of sick pigs have no significant difference (P is more than 0.05) from that of a healthy control group, and the treatment effects are better than those of the dosage of 0.08g/kg body weight. The trial clinical trial was performed with three dose groups of 0.08g/kg body weight, 0.12g/kg body weight, 0.16g/kg body weight.

3. Experimental clinical trial

80 sick pigs and 20 healthy control pigs with typical clinical symptoms of swine lung heat cough and asthma are collected from a disease-causing pig farm for testing, and the pigs are the same in variety, unlimited in sex and close in weight in the age of days. The experiment sets up three dose groups of high, medium and low, a healthy control group and a Yinhuang oral liquid group (positive drug control group), each group has 20 heads.

To evaluate the effectiveness and safety of the Shenqing Qingshen cough-relieving granule for treating the lung heat cough and asthma of the pigs with natural clinical morbidity. The method comprises the following steps: the traditional Chinese medicine preparation Shenqingqing plague cough-relieving granule is orally taken to pigs suffering from lung heat cough and asthma with natural incidence at dosages of 0.08/kg, 0.12g/kg and 0.16/kg, 1 time a day, and 5 days after continuous taking. The oral liquid of honeysuckle flower and astragalus root is taken as a control medicine, and the effect of the Shenqingqing pestilence relieving cough granule on treating cough and asthma caused by lung heat of pigs and the influence on the growth and development of sick pigs are observed within a preset period. Results: the total effective rate of the Shenqing qingwenzhike granule treatment group can reach more than 90%, except that the Shenqing qingwenzhike granule treatment group on the 1 st day of administration has high body temperature and symptom score of the medium dosage group which are obviously higher than those of the Shenqing qingwenzhike granule low dosage group (P is less than 0.05). During the administration period, the cough number and respiratory rate and symptom score of each administration group were not significantly different (P > 0.05), and after 5 days of continuous administration, the pig body temperature, cough, respiratory rate and symptom score of each administration group were not significantly different from that of the healthy control group (P > 0.05). Conclusion: the Shenqing Qingshen cough-relieving granules with dosages of 0.08g, 0.12g and 0.16g per 1kg body weight are drenched into pigs with cough and asthma caused by lung heat and naturally occurring, and can effectively treat the cough and asthma caused by lung heat of the pigs for 5 days, the cure rate is 80%, 80% and 85%, the total effective rate is 90%, 95% and 95% respectively, the spirit of the sick pigs is recovered quickly, the body temperature is normal, the breath is stable, the urine and the feces are normal, and various clinical symptoms disappear. Considering comprehensively, the recommended dosage of the Shenqing Qingshen cough relieving granule for clinical application is recommended to take 0.12g/kg body weight for 5 days.

4. Expanding clinical trial

And collecting at least 100 sick pigs with typical clinical symptoms of lung heat cough and asthma from a relevant sick pig farm for test, wherein the clinical symptoms and the test indexes of the sick pigs are in accordance with inclusion standards, and the same variety and sex are unlimited. 50 heads of the test group (Shenqing qingwenzhike granule) and 50 heads of the positive control group (Yinhuang oral liquid).

In order to examine and evaluate the effectiveness and the safety of the Shenqing Qingshen cough granule for treating the cough and asthma caused by lung heat of pigs in a larger range. The method comprises the following steps: pigs suffering from lung heat cough and asthma with natural incidence are randomly grouped after being screened and determined according to clinical inclusion standards, and are freely drunk by respectively dissolving the Shenqing qingwen cough-relieving granules into 2g/L drinking water dosage and diluting the Yinhuang oral liquid into 1mL/L drinking water dosage, and are continuously treated for 5 days. The effectiveness and the safety of the Shenqing Qingshen cough-relieving granule for treating the cough and asthma caused by lung heat of pigs are comprehensively judged by measuring the body temperature, the cough frequency for 5min, the respiratory rate, the ingestion and water intake, the nasal liquid shape, the ear temperature and skin color, the conjunctival color, the urine and stool shape, other clinical symptoms and the like. Results: after taking the medicine, the clinic symptoms of the pigs suffering from the diseases are quickly improved and cured, the spirit is gradually active, the body temperature is reduced to be normal, the skin color and conjunctiva color are recovered to be normal, the cough is reduced until the cough disappears, the respiratory frequency is reduced to be normal, the nasal fluid is reduced and disappears, the appetite is gradually recovered, the appetite is reduced, and the urine and the stool are gradually recovered to be normal; the recovery conditions of the Shenqing qingwenzhike granule group body temperature, respiratory rate, symptom score and the like are obvious or extremely obvious superior to those of the Yinhuang oral liquid group (P is less than 0.05 or P is less than 0.01). Conclusion: the Shenqing granule for clearing plague and relieving cough can effectively treat cough and asthma caused by lung heat of pigs, the cure rate is 82%, the total effective rate can reach 98%, the cure rate is basically equivalent to 78% and 94% of the total effective rate of the Yinhuang oral liquid group, and symptoms are relieved or disappeared quickly in the process of medication. Based on the above conclusion, in order to facilitate clinical practical application, it is recommended that the clinically recommended dose of 2g/L drinking water is dissolved and then added into drinking water for free drinking, and the administration is continued for 5 days.

The invention has the following innovation points and beneficial effects:

1. innovations compared to existing homogenous formulations.

By referring to the data, 15 compound medicines with the functions of clearing heat and detoxicating, relieving cough, reducing sputum and relieving asthma are obtained and are used for treating cough and asthma caused by lung heat of pigs. The details are as follows:

(1) The Eryu Donghua powder consists of rhizoma anemarrhenae, flos Farfarae, bulbus Fritillariae Thunbergii, radix Platycodi, semen Armeniacae amarum, fructus Aristolochiae, scutellariae radix, cortex Mori, radix Stephaniae Cepharanthae, flos Lonicerae and radix Curcumae. Has effects of clearing heat, moistening lung, relieving cough and eliminating phlegm. For treating cough due to lung heat of animals, 250-300g of horses and cattle; 40-80g of sheep and pigs.

(2) Two Chen San are prepared from rhizoma Pinelliae Preparada, pericarpium Citri Tangerinae, poria, and Glycyrrhrizae radix. Has effects of eliminating dampness, resolving phlegm, regulating qi-flowing and regulating stomach. Can be used for treating cough, emesis, and abdominal distention due to damp phlegm in animals. 150-200g of horses and cattle; sheep and pig 30-45g.

(3) Cough relieving powder comprises rhizoma anemarrhenae, fructus Aurantii, herba Ephedrae, radix Platycodi, semen Armeniacae amarum, semen Lepidii, cortex Mori, pericarpium Citri Tangerinae, gypsum Fibrosum, radix Peucedani, rhizoma Belamcandae, folium Eriobotryae, and Glycyrrhrizae radix. Has the effects of clearing lung-heat, resolving phlegm, relieving cough and asthma. Can be used for treating cough and asthma due to lung heat of animals. 250-300g of horses and cattle; sheep and pig 45-60g.

(4) Alumen powder comprises Alumen, bulbus Fritillariae Thunbergii, coptidis rhizoma, radix Angelicae Dahuricae, radix Curcumae, scutellariae radix, radix et rhizoma Rhei, semen Lepidii, and Glycyrrhrizae radix. Has effects of clearing heat, eliminating phlegm, descending qi and relieving asthma. Can be used for treating cough and asthma due to lung heat of animals. 250-300g of horses and cattle; 40-80g of sheep and pigs; 1-3g of rabbits and birds.

(5) Lily powder for strengthening gold comprises Bulbus Lilii, radix Paeoniae alba, radix Angelicae sinensis, glycyrrhrizae radix, radix scrophulariae, bulbus Fritillariae Cirrhosae, radix rehmanniae Preparata, radix Platycodi, and radix Ophiopogonis. Has effects of nourishing yin, clearing heat, moistening lung and relieving cough. Can be used for treating cough and asthma due to deficiency of lung and fire excess due to yin deficiency, and laryngopharynx swelling and pain. 250-300g of horses and cattle; sheep and pig 45-60g.

(6) The flos Lonicerae asthma relieving powder comprises flos Daturae Metelis, herba Ephedrae, semen Armeniacae amarum, gypsum Fibrosum, alumen, and has effects of relieving asthma and relieving cough. Can be used for treating asthma and cough in animals. 250-300g of horses and cattle; sheep and pig 45-60g.

(7) Asthma relieving powder comprises cortex Mori, parched semen Armeniacae amarum, raphani semen, semen Lepidii, fructus Perillae, radix Codonopsis, atractylodis rhizoma (parched), caulis Aristolochiae Manshuriensis, radix et rhizoma Rhei, radix Curcumae, scutellariae radix, and fructus Gardeniae. Has effects of clearing lung-heat, relieving cough and relieving asthma. Can be used for treating cough and asthma due to lung heat of animals. 200-350g of horses and cattle; 30-50g of sheep and pigs; 1-3g of rabbits and birds.

(8) A powder for regulating lung and relieving cough comprises Bulbus Lilii, radix Ophiopogonis, purified pinellia Tuber, radix Asteris, glycyrrhrizae radix, cortex et radix Polygalae, rhizoma anemarrhenae, radix Glehniae, pericarpium Citri Tangerinae, poria, and Pumex, and has effects of moistening lung, resolving phlegm, and relieving cough. Can be used for treating cough due to yin deficiency and chronic cough due to injury of animals. 250-300g of horses and cattle; 40-60g of sheep and pigs.

(9) An oral liquid of YINHUANG extract comprises flos Lonicerae and Scutellariae radix extract. Has effects of clearing heat, dispelling pathogenic wind, relieving sore throat and removing toxic substances. Can be used for treating wind-heat affecting lung, fever and cough of animals. Every 1L of water, 1ml of pigs and chickens are used for 3 days.

(10) A Chinese medicinal powder, MAXINGSHIGAN powder, comprises herba Ephedrae, semen Armeniacae amarum, gypsum Fibrosum, and Glycyrrhrizae radix. Has effects of clearing heat, dispersing lung qi, and relieving asthma. Can be used for treating cough and asthma due to lung heat of animals. 200-300g of horses and cattle; 30-60g of sheep and pigs; 1-3g of rabbits and birds.

(11) A powder for clearing lung-heat and relieving cough comprises cortex Mori, rhizoma anemarrhenae, semen Armeniacae amarum, radix Peucedani, flos Lonicerae, fructus forsythiae, radix Platycodi, glycyrrhrizae radix, exocarpium Citri rubrum, and Scutellariae radix. Has the effects of clearing away lung heat, resolving phlegm and relieving cough. Can be used for treating cough and asthma due to lung heat and laryngopharynx swelling and pain. 200-300g of horses and cattle; 30-50g of sheep and pigs; 1-3g of rabbits and birds.

(12) The lung-heat clearing granule comprises radix Isatidis, semen Lepidii, bulbus Fritillariae Thunbergii, radix Platycodi and Glycyrrhrizae radix, and has effects of clearing lung-heat, relieving asthma, eliminating phlegm and relieving cough. Can be used for treating cough and asthma due to lung heat and laryngopharynx swelling and pain. The dosage is 20-40g for one time, 2 times a day, and 3-5 days.

(13) A powder, DIGANZHIHUANG powder, comprises radix Platycodi, radix Sophorae Tonkinensis, fructus Gardeniae, radix Sophorae Flavescentis, and Scutellariae radix. Has effects of clearing lung-heat, relieving cough, detumescence and relieving sore throat. Can be used for treating cough and asthma due to lung heat and laryngopharynx swelling and pain. 50-150g of horses and cattle; 10-30g of sheep and pigs; 2-3g of rabbits and birds.

(14) Herba Ephedrae and radix Sophorae Flavescentis powder, comprises herba Ephedrae, radix Sophorae Flavescentis, scutellariae radix, radix Stemonae, radix Isatidis, radix Sophorae Tonkinensis and Glycyrrhrizae radix. Has effects of clearing heat, eliminating phlegm, relieving cough and asthma. Can be used for treating fever, asthma, cough, excessive phlegm, etc. caused by upper respiratory tract infection of animals. Mixed feeding: 8-10g of pig per 1kg of feed.

(15) A Chinese medicinal composition, QINGREJIEDU powder, comprises radix et rhizoma Rhei, scutellariae radix, coptidis rhizoma, cortex Phellodendri, rhizoma Menispermi, herba Taraxaci, glycyrrhrizae radix, gypsum Fibrosum, radix Polygoni Ciliinerve, herba Artemisiae Scopariae, herba Siraitiae Grosvenorii, bupleuri radix, and herba Ephedrae. Has effects of clearing heat, detoxicating, detumescence and relieving pain. Can be used for treating excessive triple energizer fire, cough due to lung heat, jaundice due to damp-heat, sore throat, and aphtha of the mouth and tongue. 150-300g of horses and cattle; 250-400g of camel; sheep and pig 10-20g.

In conclusion, the prescription taking bistort rhizome, dyers woad leaf, giant knotweed rhizome and myrobalan as main medicinal materials has not been found in a plurality of prescriptions with the effects of clearing heat and detoxicating, resolving phlegm and relieving cough and asthma, and has novelty and creativity.

2. The formula is novel and innovative in the theory of traditional Chinese medicine. Bistort rhizome has the effects of clearing heat and detoxicating and clearing lung heat, and is a monarch; the dyers woad leaf has the effects of clearing heat and detoxicating, and helps bistort rhizome to clear heat and detoxify, and is minister; the giant knotweed rhizome has the effects of relieving cough, resolving phlegm, clearing heat and detoxicating, on one hand, the assistant and monarch drug has the effects of clearing lung heat and relieving cough, and on the other hand, the assistant and ministerial drug has the effects of clearing heat and detoxicating, and is an adjuvant drug; the myrobalan has the effects of astringing lung and relieving cough, and is coordinated with the monarch drug bistort rhizome, one ascending and one descending, one breathing and one opening and one closing, and the balance, so that the medicine has ingenious and proper compatibility, is used as an adjuvant drug together with the giant knotweed, has the effects of carrying medicine upwards, guiding the medicine into the lung and harmonizing the medicines, and plays roles of guiding the medicine and guiding the medicine. The medicines are combined together to play roles of clearing heat and detoxicating, resolving phlegm and relieving cough and asthma.

3. The main functions of the traditional Chinese medicine composition are as follows: clearing away heat and toxic materials, eliminating phlegm, relieving cough and asthma. Can be used for treating cough and asthma due to lung heat of pig. The functional indications are determined by clinical and extended clinical trials based on pharmacodynamic, toxicological and pharmaceutical studies. The test data confirm that: definite function, special main cure, convenient usage, reasonable dosage and safe and effective treatment effect. So far, the same report for treating cough and asthma caused by lung heat of pigs is not seen. Has novelty and clinical treatment practicability.

4. The four medicinal materials in the formula of the traditional Chinese medicine composition are extractum extracted by aqueous alcohol and water, the extractum is sprayed on auxiliary materials to be made into particles, all active ingredients are released from plant cells, and the effective ingredients are administered in a drinking water mode after being dissolved in water, so that the traditional Chinese medicine composition is easy to accept by sick pigs, and after the pigs drink, a part of the effective ingredients are absorbed by mucous membrane under the mouth and tongue, a part of the effective ingredients are absorbed by the stomach, and the rest of the effective ingredients are completely absorbed in intestinal tracts, so that the digestion and the absorption are sufficient and rapid, and the treatment effect is rapid and lasting. Meanwhile, the traditional Chinese medicine granule has mature and simple preparation process, stable quality, low production cost, easy storage and long-distance transportation of the granule and suitability for large-scale application in animal husbandry.

5. The traditional Chinese medicine composition provided by the invention has the dosage of 0.12g/kg, is used for a diseased pig of 60kg, has the dosage of only 7.2g in one day, and is only 1/6 of the average daily dosage of 45g compared with the 15 heat-clearing and cough-relieving compound preparations, so that the traditional Chinese medicine composition is small in dosage, good in curative effect and meets the new medicine requirements of three effects and three hours. In the expanded clinical test, the medicine is dissolved and prepared into 2g/L water solution according to the dosage, and the water solution is added into drinking water to allow pigs to drink freely, and the medicine is continuously taken for 5 days. The cure rate of the cough and asthma caused by the lung heat of the pigs is 82%, the total effective rate can reach 98%, the cure rate is basically equivalent to 78% and 94% of the total effective rate of the Yinhuang oral liquid group, and the symptoms are quickly relieved or disappeared in the process of medication. Is based on the 'Chinese medicine is a great treasury, which should be developed and improved' and developed and improved new veterinary medicine without antibiotic.

The specific embodiment of the invention is as follows:

example 1

Prescription: 300 parts of bistort rhizome, 300 parts of myrobalan, 120 parts of giant knotweed, 280 parts of dyers woad leaf

The preparation method comprises the following steps: taking bistort rhizome, myrobalan, giant knotweed rhizome and dyers woad leaf according to the weight part ratio of the composition, crushing, sieving with a 80-mesh sieve, uniformly mixing, split charging, and preparing into powder for later use.

Taking standby powder, adding water into 2g/L mixed solution according to the weight of a sick pig and the dosage of 0.12g per 1kg, adding into drinking water, and taking by the pig by free drinking water for 5 days to obtain the treatment effect of 95.2% of total effective rate of lung heat cough and asthma of the pig.

Example 2

Prescription: 400 parts of myrobalan 200 parts, giant knotweed 100 parts and dyers woad leaf 300 parts.

The preparation method comprises the following steps: taking bistort rhizome, myrobalan and giant knotweed rhizome according to the weight part ratio of the composition, crushing the bistort rhizome, the myrobalan and the giant knotweed rhizome into blocks with the size of 0.5 cm-0.8 cm, mixing the blocks with dyers woad leaf, adding 70% ethanol, carrying out reflux extraction for 2 times, each time for 2-2.5 hours, filtering, recovering ethanol from filtrate, and obtaining clear paste for later use; adding 6 times of water into the residues, decocting for 1-1.5 hours, filtering, concentrating the filtrate under reduced pressure to obtain fluid extract with the relative density of 1.05 (60 ℃), mixing with the ethanol extract, and continuously concentrating under reduced pressure to obtain fluid extract with the relative density of 1.10 (60 ℃), for later use; mixing dextrin 260g and sucrose 480-520 g, spraying with the prepared fluid extract as adhesive, and granulating 1000g according to a prescription.

Taking the standby granules, and taking the granules according to the weight of a sick pig, wherein the dosage of 0.12g is administered per 1kg, preparing 2g/L solution by using water, adding the solution into drinking water, and taking the solution by the pig through free drinking water for 5 days, thereby obtaining the treatment effect of 98.0% of total effective rate of lung heat cough and asthma of the pig.

Example 3

Prescription: 450 parts of bistort rhizome, 150 parts of myrobalan, 150 parts of giant knotweed rhizome and 250 parts of dyers woad leaf

The preparation method comprises the following steps: taking bistort rhizome, myrobalan and giant knotweed rhizome according to the weight part ratio of the composition, crushing the bistort rhizome, the myrobalan and the giant knotweed rhizome into blocks with the size of 0.5 cm-0.8 cm, mixing the blocks with dyers woad leaf, adding 70% ethanol, carrying out reflux extraction for 2 times, each time for 2-2.5 hours, filtering, recovering ethanol from filtrate, and obtaining clear paste for later use; adding 6 times of water into the residues, decocting for 1-1.5 hours, filtering, concentrating the filtrate under reduced pressure to obtain clear paste with the relative density of 1.05 (60 ℃), mixing with the alcohol extract, uniformly mixing, refrigerating and standing for 24 hours, filtering, diluting the filtrate with distilled water to 1g of raw medicinal material per 1ml, filling, sterilizing, and preparing into oral liquid for later use.

Taking the standby oral liquid, adding the standby oral liquid into drinking water according to the weight of a sick pig at the dosage of 0.12ml for each 1kg, and taking the standby oral liquid by the pig in free drinking water for 5 days to obtain the treatment effect of the total effective rate of lung heat cough and asthma of the pig of 95.8%.

Example 4

Prescription: 500 parts of bistort rhizome, 200 parts of myrobalan, 100 parts of giant knotweed rhizome and 200 parts of dyers woad leaf

Taking standby powder, adding water into 2g/L mixed solution according to the weight of a sick pig and the dosage of 0.12g per 1kg, adding into drinking water, and taking by the pig by free drinking water for 5 days to obtain the treatment effect of 95.6% of total effective rate of lung heat cough and asthma of the pig.

Example 5

Prescription: 250 parts of bistort rhizome, 300 parts of myrobalan, 100 parts of giant knotweed rhizome and 350 parts of dyers woad leaf

The preparation method comprises the following steps: according to the weight part ratio of the composition, bistort rhizome, myrobalan, giant knotweed rhizome and dyers woad leaf are taken, and prepared into granules for standby according to the preparation method of the granules.

Taking the standby granules, and preparing 2g/L solution by using water according to the dosage of 0.12g administered per 1kg of the weight of the sick pigs, adding the solution into drinking water, and taking the solution by the pigs by free drinking water for 5 days to obtain the treatment effect of 96.9% of the total effective rate of lung heat cough and asthma of the pigs.

Claims

1. The application of a traditional Chinese medicine composition in preparing a medicine for treating cough and asthma caused by lung heat of pigs is characterized in that:

250-500 parts of bistort rhizome, 100-350 parts of myrobalan, 100-200 parts of giant knotweed, and 250-350 parts of dyers woad leaf.

2. The application of a traditional Chinese medicine composition in preparing a medicine for treating cough and asthma caused by lung heat of pigs is characterized in that: 500 parts of bistort rhizome, 200 parts of myrobalan, 100 parts of giant knotweed rhizome and 200 parts of dyers woad leaf.

3. The application of a traditional Chinese medicine composition according to claim 1 in preparing a medicine for treating cough and asthma caused by lung heat of pigs, which is characterized in that: the traditional Chinese medicine composition comprises, by weight, 450 parts of bistort rhizome, 150 parts of myrobalan, 150 parts of polygonum cuspidatum and 250 parts of dyers woad leaf.

4. The application of a traditional Chinese medicine composition according to claim 1 in preparing a medicine for treating cough and asthma caused by lung heat of pigs, which is characterized in that: 400 parts of bistort rhizome, 200 parts of myrobalan, 100 parts of giant knotweed rhizome and 300 parts of dyers woad leaf.

5. The application of a traditional Chinese medicine composition according to claim 1 in preparing a medicine for treating cough and asthma caused by lung heat of pigs, which is characterized in that: 300 parts of bistort rhizome, 300 parts of myrobalan, 120 parts of giant knotweed, 280 parts of dyers woad leaf.

6. The application of a traditional Chinese medicine composition according to claim 1 in preparing a medicine for treating cough and asthma caused by lung heat of pigs, which is characterized in that: 250 parts of bistort rhizome, 300 parts of myrobalan, 100 parts of giant knotweed rhizome and 350 parts of dyers woad leaf.

7. The application of a traditional Chinese medicine composition according to claim 1 in preparing a medicine for treating cough and asthma caused by lung heat of pigs, which is characterized in that: the medicine is powder, granule and oral liquid.

8. The application of a traditional Chinese medicine composition according to claim 1 in preparing a medicine for treating cough and asthma caused by lung heat of pigs, which is characterized in that: the traditional Chinese medicine composition has the main functions of clearing heat and detoxicating, resolving phlegm and relieving cough and asthma.

9. The use of the traditional Chinese medicine composition according to claim 1 in preparing a medicament for treating cough and asthma caused by lung heat in pigs, which is characterized in that: the dosage of the medicine is that according to the weight of the sick pigs, the medicine is added with water to prepare 2g/L solution at a dosage of 0.12g crude medicine for every 1kg, and the solution is taken by the pigs by drinking water for 5 days.