CN111920898B

CN111920898B - Antiviral prescription and preparation method thereof

Info

Publication number: CN111920898B
Application number: CN202010852973.3A
Authority: CN
Inventors: 王家涛
Original assignee: Shandong Chaoyue Biotechnology Co ltd
Current assignee: Shandong Chaoyue Biotechnology Co ltd
Priority date: 2020-08-22
Filing date: 2020-08-22
Publication date: 2022-02-15
Anticipated expiration: 2040-08-22
Also published as: CN111920898A

Abstract

The invention provides an antiviral prescription, which comprises the following raw material components: radix isatidis, folium isatidis, radix bupleuri, Chinese lobelia, radix puerariae, radix codonopsis, rhizoma atractylodis macrocephalae, poria cocos, liquorice, rhizoma anemarrhenae and cortex lycii radicis. The invention also provides a preparation method of the antiviral prescription, which comprises a pretreatment step, a liquid preparation step and a post-treatment step. The antiviral prescription of the invention is effective to high fever caused by external infection and internal injury of animals, and has the advantages of rapid and stable fever reduction and no body temperature rebound phenomenon; after three days, the cure survival rate is more than or equal to 90 percent; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leukocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammation medium.

Description

Antiviral prescription and preparation method thereof

Technical Field

The invention relates to the field of antiviral prescriptions, in particular to an antiviral prescription and a preparation method thereof.

Background

In recent years, with the continuous development of large-scale and intensive cultivation, livestock and poultry viral diseases seriously affect the production of animal husbandry, which not only causes the death of livestock and poultry in large scale and the reduction of production capacity, but also threatens the health of people due to the viral diseases suffered by some people and livestock. The livestock and poultry viral diseases have high spreading speed and serious harm, and if the prevention and the treatment are not timely, the part of the patients with mild diseases die and the whole group of the patients with severe diseases cover the whole group. And some viral diseases are also immunosuppressive diseases, which cause low immunity of infected animals, occurrence of immunosuppression, and cause concurrent or secondary infection, and vaccine immunity failure, and cause extremely high elimination rate and death rate of chicken flocks, pig flocks and the like. In the large-scale cultivation in China at present, huge economic losses are caused.

Since the virus itself lacks an enzyme system, it can only be strictly parasitic in living cells. While killing viruses, chemical drugs often target the host. The host cells cause a certain degree of side effects. And chemical drugs have the defects of drug resistance, toxic and side-effect component residue and the like, and have no specific drugs for various viral infectious diseases, and the curative effect is not ideal. After WTO is added, nearly one hundred kinds of medicines for disease control are listed as forbidden products in export animal production areas in China. Therefore, people turn their attention to the antiviral properties of natural medicinal components, and the research on the properties of natural medicinal components has risen internationally. The natural medicinal components can not only directly kill virus, but also adjust the immunity of organism and improve the antivirus ability of organism. And the natural medicinal components hardly generate drug resistance, have no toxic and side component residues, and meet the requirement of green, healthy and sustainable development of the era.

The applicant researches and discovers that when the existing natural medicine antiviral prescription is used for high fever of an animal body caused by external infection or internal injury, the fever is not stable, and the phenomenon of body temperature rebound is frequent; moreover, the antiviral prescription cannot rapidly stimulate the animal body to generate white blood cells capable of enhancing the lymphatic system, and the blocking, generating and releasing effects on inflammatory mediators of the animal body are not ideal.

Chinese patent CN109846987A discloses an antiviral compound traditional Chinese medicine for livestock and poultry, a preparation method and application thereof, which adopts components such as burdock, mint, chrysanthemum, ephedra, dyers woad leaf, radix isatidis and the like to prevent and treat viral diseases of livestock and poultry. The disadvantages are that: in practical application, the livestock and poultry antiviral compound traditional Chinese medicine is used for treating high fever caused by external infection or internal injury of animal organisms, the fever is not stable, and the phenomenon of body temperature rebound is frequent; moreover, the antiviral prescription cannot rapidly stimulate the animal body to generate white blood cells capable of enhancing the lymphatic system, and the blocking, generating and releasing effects on inflammatory mediators of the animal body are not ideal.

Disclosure of Invention

In order to solve the technical problems in the prior art, the invention provides an antiviral prescription and a preparation method thereof, which are used for stably abating fever and preventing the body temperature from rebounding when the animal body is subjected to high fever caused by external infection or internal injury; in addition, the antiviral prescription can rapidly stimulate the animal organism to generate white blood cells capable of enhancing the lymphatic system, and effectively block the generation and release of inflammatory mediators of the animal organism.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

an antiviral prescription comprises the following raw material components in parts by weight: 400-500 parts of isatis root, 150-250 parts of dyers woad leaf, 80-120 parts of radix bupleuri, 80-120 parts of Chinese lobelia, 100-200 parts of kudzu root, 80-120 parts of codonopsis pilosula, 50-100 parts of bighead atractylodes rhizome, 70-100 parts of poria cocos, 30-60 parts of liquorice, 50-60 parts of rhizoma anemarrhenae and 20-50 parts of cortex lycii radicis.

Radix Isatidis is dried root of Isatis Indigotica of Brassicaceae. Has the effects of clearing away heat and toxic materials, cooling blood, and relieving sore throat. Mainly treats exogenous fever, early warm disease, swollen and sore throat, warm poison and macula, mumps, erysipelas, carbuncle and sore toxin.

Folium Isatidis is plant Isatis tinctoria and radix Isatidis of Brassicaceae, plant Polygonum tinctorium of Polygonaceae, and plant Indigofera tinctoria of Leguminosae. The main treatment is as follows: macula due to toxic heat, erysipelas, sore throat, aphtha of the mouth and tongue, skin ulcer, carbuncle, and toxic swelling. It is also indicated for phlegm-heat stagnation in lung, yellow and thick sputum.

The medicinal part of bupleuri radix is dried root of Bupleurum scorzonerifolium or Bupleurum scorzonerifolium of Umbelliferae. Exterior-releasing herbs are commonly used. Bitter in property and slightly cold in nature, entering liver and gallbladder meridians. Has the functions of harmonizing exterior and interior, soothing liver and invigorating yang. Can be used for treating common cold, fever, malaria, stagnation of qi due to depression of the liver, distending pain of chest and hypochondrium, rectocele, uterine prolapse, and menoxenia.

Lobelia chinensis, a perennial herb of Lobelia in Campanulaceae. The traditional Chinese medicine composition has the effects of clearing heat and removing toxicity, and inducing diuresis to reduce edema, and has the effects of clearing heat and removing toxicity of heart, small intestine and lung channels, has good anti-inflammatory effect besides the effects of improving the effects of clearing heat and removing toxicity of isatis root and dyers woad leaf, can cause various serious inflammatory reactions such as congestion, bleeding, necrosis, high fever, edema, diarrhea and the like of various organs after a virus infects organisms, prevents the failure of various organs due to inflammation, and greatly improves the tolerance of the organisms to pathogens.

Radix Puerariae is dried root of Pueraria lobata Ohwi of Leguminosae. Sweet, pungent and cool. Has the functions of expelling pathogenic factors from muscles, allaying fever, promoting eruption, promoting the production of body fluid to quench thirst, invigorating yang and stopping diarrhea. It is commonly indicated for exterior syndrome with fever, stiffness and pain of neck and back, measles without adequate eruption, thirst due to fever, diabetes due to yin deficiency, dysentery due to heat-purging and diarrhea due to spleen deficiency.

Radix Codonopsis belonging to genus Codonopsis of family Campanulaceae, genus Codonopsis, and having milky juice. Is a traditional tonifying medicine commonly used in China and has the efficacies of tonifying middle-jiao and Qi, invigorating spleen and benefiting lung.

Atractylodis rhizoma, a perennial herb of Atractylodes of Compositae. Being bitter, warm, pungent and drastic in property, it has the actions of drying dampness, resolving turbidity and alleviating pain.

Poria is dried sclerotium of Poria cocos (Schw.) wolf of Polyporaceae. Sweet and bland in flavor and mild in nature. It enters heart, lung, spleen and kidney meridians. Has the functions of promoting diuresis, invigorating spleen and calming heart. Can be used for treating edema, oliguria, phlegm and fluid retention, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.

Glycyrrhiza uralensis, Leguminosae, Glycyrrhiza genus perennial herbs. Light smell, sweet and special taste. The functions are mainly used for clearing away heat and toxic material, eliminating phlegm and relieving cough, and treating abdominal cavity and the like.

Anemarrhena asphodeloides is a perennial herb. It is bitter and cold in nature. It enters lung, stomach and kidney meridians. Has the effects of nourishing yin, reducing pathogenic fire, moistening dryness, smoothing intestine, and facilitating urination and defecation. Belongs to clearing heat and expelling pathogenic fire.

Cortex Lycii is plant of Solanaceae and Lycium. Has effects of clearing heat and cooling blood, and can be used for treating consumptive disease, hectic fever, night sweat, lung heat, cough and asthma.

The compatibility of the cortex lycii radicis, the radix puerariae and the rhizoma anemarrhenae can effectively enhance the antipyretic effect, and has the characteristics of quick response, more obvious use effect and the like on hyperpyrexia and cachexia caused by various virus diseases.

The formula principle is as follows: the main formula is prepared by matching Sijunzi decoction with isatis root granules, and in order to improve the antiviral ability and reduce complications, radix bupleuri, Chinese lobelia, radix puerariae, cortex lycii radicis and rhizoma anemarrhenae are added.

Radix Isatidis, folium Isatidis, and herba Lobeliae chinensis are used as principal drugs, and have effects of clearing away heat and toxic materials and relieving inflammation; radix Puerariae, cortex Lycii, rhizoma anemarrhenae are ministerial drugs, have effects of defervescing; radix Codonopsis, Atractylodis rhizoma, Poria and Glycyrrhrizae radix are adjuvant drugs, and have effects of protecting liver, invigorating spleen, relieving cough, rapidly increasing food intake, promoting organism function recovery, enhancing immunity, regulating immunity, and increasing phagocytosis of immune cells of lymphatic system. The codonopsis pilosula is a guiding drug and has the effects of harmonizing exterior and interior, soothing liver, invigorating yang and balancing and regulating organism balance.

A preparation method of an antiviral prescription comprises a pretreatment step, a liquid preparation step and a post-treatment step.

The pretreatment step comprises a processing step. In the processing step, the selected medicinal materials are processed according to the processing standard of the traditional Chinese medicine decoction pieces in Shandong province, and the processed medicinal materials are mixed.

And in the liquid preparation step, water is added into the mixed medicinal materials, and the decoction is carried out for three times. The first decoction is carried out, water with the volume 10 times that of the medicinal materials is added, the decoction is carried out for 1 hour, and the filtration is carried out to obtain filtrate; the secondary decoction is carried out, water with the volume 10 times that of the medicinal materials is added again, the decoction is carried out for 1 hour, and the filtration is carried out to obtain the filtrate; and (3) decocting for three times, adding water with the volume 8 times that of the medicinal materials again, decocting for 1 hour, and filtering to obtain filtrate. And finally, mixing the filtrates obtained by the three times of decoction to obtain liquid medicine, and concentrating the liquid medicine into thick paste to obtain concentrated liquid medicine.

And the post-treatment step is used for post-treating the concentrated liquid medicine prepared in the liquid preparation step.

The post-treatment can be a granulation and drying step; or standing, concocting, sterilizing and bottling.

And (3) granulating, namely adding sucrose and dextrin into the concentrated liquid medicine, uniformly mixing, and then preparing into granules. The concentrated liquid medicine is as follows: sucrose: the weight ratio of the dextrin is 1-2: 5-6: 3-5.

And drying, namely drying the particles until the moisture content is less than 2%, and preparing the granules.

And standing, namely standing the concentrated liquid medicine for 48 hours.

And (3) preparing, filtering the liquid medicine after standing, adding a predetermined part of water, sterilizing and filling to obtain the oral liquid. The filtrate: the ratio of the weight parts of the water is 1-3: 60-80.

Preferably, the raw materials of the antiviral prescription further comprise 20-30 parts of wheat seedling juice and 10-20 parts of a brown cloud agate snail extract.

The wheat seedling juice is prepared by cutting and crushing fresh wheat seedling leaves which are aquatic for 10-15 days at 200-300 RPM for 1-3 min, rapidly heating to 70-80 ℃, preserving heat for 0.5-1 h, squeezing and filtering.

Collecting mucus secreted by feet of the brown cloudy agate snails, quickly cooling to-2 to-3 ℃, and preserving heat for 2-4 hours; shearing the mucus at 200-300 RPM for 10-15 min; filtering out solids in the mucus; centrifuging the mucus at 6000-70000 RPM for 8-10 min, and taking supernatant; and freeze-drying the supernatant at a low temperature under a vacuum condition until the water content is less than 3%, and crushing to 80-100 meshes to obtain the brown cloud agate snail extract.

The preparation method of the antiviral prescription comprises a crushing step and a microwave treatment step after the processing in the pretreatment step.

And in the crushing step, the processed mixed medicinal materials are crushed to 80-100 meshes.

And the microwave treatment step is to carry out microwave treatment on the crushed mixed medicinal materials. In the microwave treatment, the microwave radiation power is 300-350W, the microwave frequency is 1800-2200MHz, and the microwave treatment time is 30-40 s.

The liquid preparation step comprises the steps of extraction, separation, equilibrium and distillation.

And in the extraction, a supercritical carbon dioxide extraction process is adopted to extract the mixed medicinal materials prepared in the pretreatment step to prepare an extract. The extraction pressure is 16-36 MPa, the extraction temperature is 50-60 ℃, the extraction time is 2-3 h, and the flow of carbon dioxide is 1500-1800L/h.

And (4) performing separation, namely performing reduced pressure separation twice after extraction. The first decompression separation is carried out, the separation pressure is 10-15 MPa, and the separation temperature is 30-40 ℃; and (3) carrying out secondary decompression separation, wherein the separation pressure is 3-5 MPa, and the separation temperature is 30-40 ℃.

And balancing, namely uniformly spraying water to the separated extract, spraying a predetermined part of wheat seedling juice, and standing for 3-4 hours. The ratio of the water to the extract in parts by weight is 2-3: 1.

And the distillation adopts two-stage distillation. The first-stage distillation is carried out, wherein the distillation pressure is 70-80 KPa, the distillation temperature is 80 ℃, and the distillation time is 1-2 h, so as to obtain a first-stage light component and a first-stage heavy component; and in the secondary distillation, the primary heavy component is subjected to secondary distillation, the distillation pressure is increased to 40-50 KPa, the distillation temperature is 70 ℃, the distillation time is 0.5-1 hour, a secondary light component and a secondary heavy component are obtained, and the secondary heavy component is filtered to obtain the concentrated liquid medicine.

The application of the antiviral prescription comprises the following granules: once a day, every 1L of water, the amount of chicken is 1g, the amount of pig is 10g, the amount of horse and cattle is 50g, and water is freely drunk.

The oral liquid comprises the following components: once a day, every 1L of water, the amount of chicken is 1.5ml, the amount of pig is 15ml, the amount of horse and cattle is 70ml, and water is freely drunk.

Compared with the prior art, the invention has the beneficial effects that:

(1) the antiviral prescription of the invention is effective on high fever caused by external infection and internal injury of animals, and has the advantages of rapid and stable fever reduction, and the body temperature rebound phenomenon is less than 0.5%;

(2) the antiviral prescription of the invention is used for treating high fever caused by external infection and internal injury of animals for three days continuously, and the cure survival rate is more than or equal to 90 percent;

(3) the antiviral prescription of the invention has comprehensive antiviral effects of clearing away heat and toxic materials, strengthening heart and rescuing lung, protecting liver and invigorating spleen, and also has the effects of promoting development of immune organs and improving the immunity of animal organisms;

(4) the antiviral prescription of the invention can rapidly stimulate animal organisms to generate white blood cells, increase phagocytosis of immune cells of a lymphatic system and effectively block generation and release of inflammatory mediators;

(5) the antiviral prescription has remarkable treatment effects on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases;

(6) the antiviral prescription also has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 11-13%;

(7) the antiviral prescription of the invention has safe and convenient use. Has obvious curative effect and no drug residue, and meets the current ecological, safe and green animal husbandry production requirements and the consumption requirements of consumers on animal husbandry products.

Detailed Description

In order to more clearly understand the technical features, objects, and effects of the present invention, specific embodiments of the present invention will now be described.

Example 1

An antiviral prescription comprises the following raw material components in parts by weight: 400 parts of isatis root, 150 parts of dyers woad leaf, 120 parts of radix bupleuri, 80 parts of Chinese lobelia, 200 parts of kudzu root, 80 parts of codonopsis pilosula, 50 parts of bighead atractylodes rhizome, 100 parts of poria cocos, 30 parts of liquorice, 60 parts of rhizoma anemarrhenae and 50 parts of cortex lycii radicis.

The post-treatment comprises the steps of granulation and drying.

And (3) granulating, namely adding sucrose and dextrin into the concentrated liquid medicine, uniformly mixing, and then preparing into granules. The concentrated liquid medicine is as follows: sucrose: the weight ratio of dextrin is 1:5: 3.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.3% body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 11 percent.

Example 2

An antiviral prescription comprises the following raw material components in parts by weight: 450 parts of isatis root, 200 parts of dyers woad leaf, 100 parts of radix bupleuri, 100 parts of Chinese lobelia, 150 parts of kudzu root, 100 parts of codonopsis pilosula, 100 parts of bighead atractylodes rhizome, 100 parts of poria cocos, 50 parts of liquorice, 50 parts of rhizoma anemarrhenae and 50 parts of cortex lycii radicis.

The post-treatment comprises the steps of granulation and drying.

And (3) granulating, namely adding sucrose and dextrin into the concentrated liquid medicine, uniformly mixing, and then preparing into granules. The concentrated liquid medicine is as follows: sucrose: the weight ratio of dextrin is 1:5: 5.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.2% body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 13 percent.

Example 3

The post-treatment comprises the steps of standing, blending, sterilizing and filling.

And standing, namely standing the concentrated liquid medicine for 48 hours.

And (3) preparing, filtering the liquid medicine after standing, adding a predetermined part of water, sterilizing and filling to obtain the oral liquid. The filtrate: the weight ratio of water is 1: 80.

The application of the antiviral prescription comprises the following components: once a day, every 1L of water, the amount of chicken is 1.5ml, the amount of pig is 15ml, the amount of horse and cattle is 70ml, and water is freely drunk.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.3% body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 12 percent.

Example 4

And standing, namely standing the concentrated liquid medicine for 48 hours.

And (3) preparing, filtering the liquid medicine after standing, adding a predetermined part of water, sterilizing and filling to obtain the oral liquid. The filtrate: the ratio of the weight parts of the water is 1: 70.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.2% body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 12 percent.

Example 5

An antiviral prescription comprises the following raw material components in parts by weight: 400 parts of isatis root, 150 parts of dyers woad leaf, 120 parts of radix bupleuri, 80 parts of Chinese lobelia, 200 parts of kudzu root, 80 parts of codonopsis pilosula, 50 parts of bighead atractylodes rhizome, 100 parts of poria cocos, 30 parts of liquorice, 60 parts of rhizoma anemarrhenae, 50 parts of cortex lycii radicis, 20 parts of wheat seedling juice and 20 parts of a brown Yunnan agate snail extract.

The wheat seedling juice is prepared by cutting fresh wheat seedling leaves in a water-borne 10-day period at 200RPM, crushing for 1min, rapidly heating to 70 ℃, preserving heat for 0.5h, squeezing, and filtering.

Collecting mucus secreted by feet of the brown agate snails, quickly cooling to-2 ℃, and preserving heat for 4 hours; shearing the mucus at 200RPM for 10 min; filtering out solids in the mucus; centrifuging the mucus at 6000RPM for 8min to obtain supernatant; and (3) freeze-drying the supernatant at low temperature under vacuum condition until the water content is less than 3%, and crushing to 80 meshes to obtain the brown cloud agate snail extract.

The pretreatment step comprises a processing step. In the processing step, the medicinal materials except the wheat seedling juice are processed according to the processing standard of the traditional Chinese medicine decoction pieces in Shandong province, and the processed medicinal materials are mixed.

The pretreatment step also comprises a crushing step and a microwave treatment step after processing.

And in the crushing step, the processed mixed medicinal materials are crushed to 80 meshes.

And the microwave treatment step is to carry out microwave treatment on the crushed mixed medicinal materials. In the microwave treatment, the microwave radiation power is 300W, the microwave frequency is 1800MHz, and the microwave treatment time is 30 s.

And in the extraction, a supercritical carbon dioxide extraction process is adopted to extract the mixed medicinal materials prepared in the pretreatment step to prepare an extract. The extraction pressure is 16MPa, the extraction temperature is 50 ℃, the extraction time is 2h, and the carbon dioxide flow is 1500L/h.

And (4) performing separation, namely performing reduced pressure separation twice after extraction. The primary decompression separation is carried out, the separation pressure is 10MPa, and the separation temperature is 30 ℃; and (3) carrying out secondary decompression separation, wherein the separation pressure is 3MPa, and the separation temperature is 30 ℃.

And (3) balancing, uniformly spraying water to the separated extract, spraying a predetermined part of wheat seedling juice, and standing for 3 hours. The ratio of the weight parts of the water to the extract is 2: 1.

And the distillation adopts two-stage distillation. The first-stage distillation is carried out, wherein the distillation pressure is 70KPa, the distillation temperature is 80 ℃, and the distillation time is 1h, so as to obtain a first-stage light component and a first-stage heavy component; and the second-stage distillation is to carry out second-stage distillation on the first-stage heavy component, the distillation pressure is increased to 40KPa, the distillation temperature is 70 ℃, the distillation time is 0.5 hour, a second-stage light component and a second-stage heavy component are obtained, and the second-stage heavy component is filtered, so that the concentrated liquid medicine is obtained.

The post-treatment comprises the steps of granulation and drying.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.1% body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 13 percent.

Example 6

And standing, namely standing the concentrated liquid medicine for 48 hours.

Example 7

An antiviral prescription comprises the following raw material components in parts by weight: 450 parts of isatis root, 200 parts of dyers woad leaf, 100 parts of radix bupleuri, 100 parts of Chinese lobelia, 150 parts of kudzu root, 100 parts of codonopsis pilosula, 100 parts of bighead atractylodes rhizome, 100 parts of poria cocos, 50 parts of liquorice, 50 parts of rhizoma anemarrhenae, 50 parts of cortex lycii radicis, 30 parts of wheat seedling juice and 20 parts of Mongolian snail extract.

The wheat seedling juice is prepared by cutting fresh wheat seedling leaves in 15-day aquatic period at 300RPM for 2min, rapidly heating to 80 deg.C, maintaining for 1h, squeezing, and filtering.

Collecting mucus secreted by feet of the brown agate snails, quickly cooling to-2 ℃, and preserving heat for 3 hours; shearing the mucus at 300RPM for 15 min; filtering out solids in the mucus; centrifuging the mucus at 6000RPM for 10min to obtain supernatant; and freeze-drying the supernatant at low temperature under vacuum condition until the water content is less than 3%, and crushing to 100 meshes to obtain the brown cloud agate snail extract.

And in the crushing step, the processed mixed medicinal materials are crushed to 100 meshes.

And the microwave treatment step is to carry out microwave treatment on the crushed mixed medicinal materials. And in the microwave treatment, the microwave radiation power is 350W, the microwave frequency is 2200MHz, and the microwave treatment time is 35 s.

And in the extraction, a supercritical carbon dioxide extraction process is adopted to extract the mixed medicinal materials prepared in the pretreatment step to prepare an extract. The extraction pressure is 20MPa, the extraction temperature is 60 ℃, the extraction time is 3h, and the flow of carbon dioxide is 1800L/h.

And (4) performing separation, namely performing reduced pressure separation twice after extraction. The primary decompression separation is carried out, the separation pressure is 12MPa, and the separation temperature is 40 ℃; and (3) carrying out secondary decompression separation, wherein the separation pressure is 3MPa, and the separation temperature is 30 ℃.

And the distillation adopts two-stage distillation. The first-stage distillation is carried out, wherein the distillation pressure is 80KPa, the distillation temperature is 80 ℃, and the distillation time is 1.2h, so as to obtain a first-stage light component and a first-stage heavy component; and the second-stage distillation is to carry out second-stage distillation on the first-stage heavy component, the distillation pressure is increased to 50KPa, the distillation temperature is 70 ℃, the distillation time is 0.5 hour, a second-stage light component and a second-stage heavy component are obtained, and the second-stage heavy component is filtered, so that the concentrated liquid medicine is obtained.

The post-treatment comprises the steps of granulation and drying.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.07 percent of body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 13 percent.

Example 8

And standing, namely standing the concentrated liquid medicine for 48 hours.

The antiviral prescription of the embodiment is effective on hyperpyrexia caused by external infection and internal injury of animals, and has the advantages of rapid and stable defervescence and 0.08% body temperature rebound phenomenon; has the comprehensive antiviral effects of clearing away heat and toxic materials, tonifying heart, rescuing lung, protecting liver, invigorating spleen, promoting development of immune organs, and improving immunity of animal body; can rapidly stimulate animal organism to generate leucocyte, increase phagocytosis of immune cells of lymphatic system, and effectively block generation and release of inflammatory medium; has remarkable treatment effect on symptoms such as high fever, tracheal (laryngeal) hemorrhage, air sacculitis, tracheal blockage, myocardial hemorrhage, lung blackening, edema, renal hemorrhage, glandular stomach swelling and the like caused by virus diseases; has the effect of improving the feed intake of animals, and the feed intake improvement rate is about 13 percent.

Example 9

The following comparative examples were set up and tested by modeling:

comparative example 1: the rest of the raw material components of Chinese lobelia, kudzu root, cortex lycii radicis and rhizoma anemarrhenae are omitted, and the method is the same as the embodiment 2;

comparative example 2: the procedure of example 7 was repeated except that "wort" was omitted from the starting materials;

comparative example 3: the process is otherwise the same as in example 7 except that the "extract of agate snails belonging to the starting material composition is omitted;

600 feather non-inoculated vaccine 20-day-old chickens provided by a chicken farm are randomly divided into 15 groups for testing, wherein each group contains 40 feathers, half of each feather and half of each sex, and the weights of the feathers are similar. The amount of Newcastle disease virus infected by each feather is 0.2ml (2.89 multiplied by 103ELD 50) each time, the virus is supplemented once after 12h interval, an artificial induction infection model is established, and the Newcastle disease virus is freely drunk according to clinical dosage for 3 days continuously.

All percentages used in the present invention are mass percentages unless otherwise indicated.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The livestock and poultry antiviral medicine is characterized by being prepared from the following raw materials in parts by weight: 400-500 parts of isatis root, 150-250 parts of dyers woad leaf, 80-120 parts of radix bupleuri, 80-120 parts of Chinese lobelia, 100-200 parts of kudzuvine root, 80-120 parts of codonopsis pilosula, 50-100 parts of bighead atractylodes rhizome, 70-100 parts of poria cocos, 30-60 parts of liquorice, 50-60 parts of rhizoma anemarrhenae, 20-50 parts of cortex lycii radicis, 20-30 parts of wheat seedling juice and 10-20 parts of brown agate snail extract;

the preparation method of the antiviral drug comprises a pretreatment step, a liquid preparation step and a post-treatment step;

the pretreatment step comprises a processing step, a crushing step and a microwave treatment step;

the processing step is to process the selected medicinal materials according to the processing specification of the Shandong province Chinese medicinal decoction pieces and mix the processed medicinal materials;

the crushing step is to crush the processed mixed medicinal materials to 80-100 meshes;

the microwave treatment step is to carry out microwave treatment on the crushed mixed medicinal materials;

the microwave treatment is carried out, wherein the microwave radiation power is 300-350W, the microwave frequency is 1800-2200MHz, and the microwave treatment is 30-40 s;

the liquid preparation step comprises the steps of extraction, separation, equilibrium and distillation;

the extraction is carried out by adopting a supercritical carbon dioxide extraction process to extract the mixed medicinal materials prepared in the pretreatment step;

the balance is carried out, after water is uniformly sprayed on the separated extract, wheat seedling juice with a preset part is uniformly sprayed on the extract, and the extract is kept stand;

and performing secondary reduced pressure distillation to obtain concentrated liquid medicine.

2. The antiviral drug for livestock and poultry according to claim 1, wherein said post-treatment step comprises post-treating the concentrated liquid medicine obtained in said liquid preparation step;

the post-treatment comprises the steps of granulation and drying;

the granulation is to add sucrose and dextrin into the concentrated liquid medicine, and to prepare granules after even mixing; the concentrated liquid medicine is as follows: sucrose: the weight ratio of the dextrin is (1-2) to (5-6) to (3-5);

3. The antiviral drug for livestock and poultry according to claim 1, wherein said post-treatment step comprises post-treating the concentrated liquid medicine obtained in said liquid preparation step;

the post-treatment comprises the steps of standing, blending, sterilizing and filling;

standing, namely standing the concentrated liquid medicine for 48 hours;

the preparation, the sterilization and the filling are carried out, the liquid medicine after standing is filtered, water with a preset part is added, and the sterilization and the filling are carried out to prepare oral liquid; the filtrate: the weight ratio of the water is (1-3) to (60-80).