CN114920598A - Biological fertilizer and preparation method and application thereof - Google Patents
Biological fertilizer and preparation method and application thereof Download PDFInfo
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- CN114920598A CN114920598A CN202210463866.0A CN202210463866A CN114920598A CN 114920598 A CN114920598 A CN 114920598A CN 202210463866 A CN202210463866 A CN 202210463866A CN 114920598 A CN114920598 A CN 114920598A
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- aminolevulinic acid
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The invention discloses a biological fertilizer and a preparation method and application thereof, wherein the biological fertilizer comprises the following components in percentage by mass: 1-50: 1 and 5-aminolevulinic acid, said use being as a biofertilizer for promoting plant growth. The chlorella extract can effectively extract and retain bioactive components in chlorella, and improves the utilization rate of the effective components of the chlorella. Meanwhile, the 5-aminolevulinic acid and the chlorella vulgaris extract are selected and compounded, so that the plant growth promoting effect can be obviously improved, the seed germination is effectively promoted, the root system activity is increased, the photosynthesis of leaves is obviously improved, the yield of crops is obviously improved, and the synergistic effect is exerted. In addition, the biological fertilizer has the advantages of few components, simple preparation process, low cost, simple use method, good effect of promoting plant growth and easy popularization and application.
Description
Technical Field
The invention belongs to the technical field of biofertilizers, relates to a biofertilizer and a preparation method and application thereof, and particularly relates to a biofertilizer containing chlorella and a preparation method and application thereof.
Background
The fertilizer makes an important contribution to the improvement of the crop yield, but the problems of quality safety and environmental safety of the fertilizer are increasingly highlighted along with the continuous increase of the using amount of the fertilizer. Particularly, in recent years, with the excessive use of fertilizers, environmental pollution and ecological deterioration are caused, phenomena of soil structure damage, organic matter content reduction and the like occur, and a series of serious ecological environment problems such as eutrophication of rivers and lakes, quality reduction of agricultural products and the like occur in partial areas. The reason for this is that there are both problems of scientific use of fertilizer and problems of quality of fertilizer. The quality safety of the fertilizer seriously restricts the sustainable development of agriculture, and directly or indirectly threatens the physical and psychological health of people. Therefore, starting from the quality safety of the fertilizer, the green and ecological environment-friendly fertilizer is greatly popularized, and the environmental pollution is effectively prevented.
The chlorella is a common unicellular green alga which is widely distributed in nature, is abundant in fresh water areas, has large biomass, can be autotrophized by utilizing light energy, can also be grown and propagated by utilizing an organic carbon source under heterotrophic conditions, and has about 15 types and more than one hundred types of varieties in the world at present. The content of the crude protein of the chlorella is particularly high and can reach more than 50 percent, particularly, the content of the crude protein of the chlorella pyrenoidosa is higher than that of the crude protein (40 percent) of the soybean, and the quality of the protein is much better. Among the essential amino acids, the contents of lysine, arginine, leucine and valine are high, the contents of phenylalanine, isoleucine and threonine are high, the content of histidine is slightly low, and the contents of methionine and tryptophan are low. Also contains abundant physiological active ingredients, such as: growth Factor (CGF), beta-carotene, chlorophyll, vitamins, trace elements, phycocyanin, unsaturated fatty acids, etc. The chlorella has high nutrient content and health care function, so that the chlorella is successively developed and used for protein source feed, food, beverage and medical products.
The 5-aminolevulinic acid serving as a key precursor substance required by chlorophyll synthesis can obviously improve the photosynthesis of leaves, obviously improve the yield of crops, and particularly induce various crops to generate tolerance to various abiotic stresses under adverse conditions of low temperature, drought, salt and alkali and the like. The cucumber seedlings are treated by adding a small amount of 5-aminolevulinic acid, the antioxidant enzyme activity of the plants is obviously improved, and the promoting effect is weakened along with the increase of the concentration. 5-aminolevulinic acid is also a novel photoactivated pesticide, can be used as a plant physiological reaction regulator, a green herbicide, an insecticide and other medicaments, and has wide application in the agricultural field.
At present, the development and patent application of chlorella biological fertilizers mostly focus on the mixing of chlorella and biological organic matters such as chemical fertilizers, bacillus, livestock excrement and straws and the like, the components of the mixed fertilizers are complex, the steps are complicated, the stability of biological activity is uncertain, and no biological fertilizer taking chlorella extract and 5-aminolevulinic acid as active components is found through retrieval.
Disclosure of Invention
In view of the above, the present invention provides a biofertilizer, a preparation method and an application thereof, wherein the biofertilizer uses chlorella vulgaris extract as a main raw material, avoids the problems of unstable activity and incomplete exertion of effective components caused by directly using chlorella vulgaris as a raw material, and has a significant plant growth promoting effect.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a biological fertilizer comprises the following components in percentage by mass of 10: 1-50: 1 and 5-aminolevulinic acid.
Preferably, the mass ratio of the chlorella extract to the 5-aminolevulinic acid is 15: 1-45: 1.
preferably, the preparation method of the chlorella extract comprises the following steps:
(1) heterotrophic culture of chlorella to obtain chlorella solution;
(2) performing enzymolysis on the algae liquid obtained in the step (1), and filtering to obtain a raw algae liquid;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Further preferably, the enzymes used in the enzymatic hydrolysis in step (2) are cellulase, pectinase, alkaline protease and fucosidase.
Further preferably, the enzymolysis in step (2) is: adding cellulase into the algae solution, and performing enzymolysis at 52-60 deg.C and pH 6.0-6.4 for 1-1.5 hr; adding pectinase, and performing enzymolysis at 48-56 deg.C and pH 3.5-4.5 for 1-2 hr; adding alkaline protease, and performing enzymolysis at 40-65 deg.C and pH 7.5-9.5 for 0.5-1 h; finally adding fucosidase, and performing enzymolysis at 48-55 deg.C and pH of 5.8-6.7 for 0.5-1.5 h.
More preferably, the addition amount of the cellulase is 0.05-0.1% of the mass of the algae liquid, the addition amount of the pectinase is 0.1-0.15% of the mass of the algae liquid, the addition amount of the alkaline protease is 0.08-0.12% of the mass of the algae liquid, and the addition amount of the fucosidase is 0.005-0.015% of the mass of the algae liquid.
The invention also provides a preparation method of the biological fertilizer, which comprises the following steps: mixing the chlorella extract and the 5-aminolevulinic acid according to the formula amount to obtain the biological fertilizer.
The invention also provides application of the biological fertilizer in promoting plant growth.
Preferably, the biofertilizer is applied by the following mode: spraying and/or irrigating roots.
Further preferably, the dilution ratio of the spray is: and (3) biological fertilizer: water 1: 500-1: 1500.
further preferably, the dilution ratio of the irrigation roots is: and (3) biological fertilizer: water 1: 300-1: 1000.
the invention also provides a biological fertilizer, which comprises the biological fertilizer or the biological fertilizer prepared by the preparation method and auxiliary materials.
The beneficial effects of the invention are as follows:
in the process of producing the alga-derived biostimulant by using the alga, on one hand, the alga body is digested to ensure that the cell wall is completely broken as far as possible, and then the content in the cell is released and the micromolecule substance which is degraded by macromolecular substance and is easy to absorb and utilize in the cell is obtained; on the other hand, the plant hormone in the plant extract is well preserved. In the early production process of the seaweed-derived biostimulant, people pay more attention to the extraction of polysaccharide, mineral elements, trace elements and other nutrient substances, and the high-temperature and high-pressure extraction mode is generally adopted, so that the phytohormone contained in the seaweed is damaged, and the growth promotion effect of the produced fertilizer on crops is not obvious.
The chlorella extract prepared by the method can effectively extract and retain bioactive components in the chlorella, and improves the utilization rate of the effective components of the chlorella.
According to the invention, the 5-aminolevulinic acid and the chlorella vulgaris extract are selected and compounded, so that the effect of promoting the growth of plants can be obviously improved, the germination of seeds is effectively promoted, the root activity is increased, the photosynthesis of leaves is obviously improved, the yield of crops is obviously improved, and the synergistic effect is exerted.
The biological fertilizer disclosed by the invention is few in components, simple in preparation process, low in cost, simple in use method, good in plant growth promoting effect and easy to popularize and apply.
Drawings
FIG. 1 is a statistical chart of plant height increase after spray treatment;
FIG. 2 is a statistical graph of plant dry weight change after spray treatment;
FIG. 3 is a statistical graph of root length variation after spraying treatment;
FIG. 4 is a statistical graph of the increase in leaf area after spray treatment;
FIG. 5 is a statistical graph of chlorophyll content changes after spray treatment;
FIG. 6 is a statistical chart of index change of strong seedlings after spraying treatment;
fig. 7 is a statistical graph of growth function changes after spray treatment.
Detailed Description
The present invention will be further explained with reference to specific examples in order to make the technical means, the technical features, the technical objectives and the effects of the present invention easier to understand, but the following examples are only preferred embodiments of the present invention, and not all embodiments of the present invention. Other embodiments obtained by persons skilled in the art without making creative efforts based on the embodiments in the implementation belong to the protection scope of the invention. In the following examples, unless otherwise specified, all the procedures and equipment used were conventional procedures, all the raw materials used were commercially available, and the purity of 5-aminolevulinic acid was 98% or more.
Example 1A biological Fertilizer
A biological fertilizer comprises the following components in percentage by mass of 10: 1 and 5-aminolevulinic acid.
The preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella liquid with the density of 30 hundred million/ml;
(2) adding cellulase 0.05 wt% of the algae solution into the algae solution, and performing enzymolysis at 52-60 deg.C and pH 6.0-6.4 for 1.5 hr; adding pectinase 0.1 wt% of the algae solution, and performing enzymolysis at 48-56 deg.C and pH of 3.5-4.5 for 2 hr; adding alkaline protease with an amount of 0.08% of the algae solution, and performing enzymolysis at 40-65 deg.C and pH 7.5-9.5 for 1 h; finally adding fucosidase accounting for 0.005% of the weight of the algae solution, and performing enzymolysis at 48-55 deg.C and pH of 5.8-6.7 for 1.5 h. Filtering to obtain raw algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the chlorella extract is mixed with 5-aminolevulinic acid to obtain the biological fertilizer.
Example 2A biofertilizer
A biological fertilizer comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella liquid with the density of 30 hundred million/ml;
(2) adding cellulase with an amount of 0.1% of the algae solution into the algae solution, and performing enzymolysis for 1h at 52-60 deg.C and pH of 6.0-6.4; adding pectinase 0.15 wt% of the algae solution, and performing enzymolysis at 48-56 deg.C and pH 3.5-4.5 for 1 hr; adding alkaline protease 0.12% of the algae solution, and performing enzymolysis at 40-65 deg.C and pH 7.5-9.5 for 0.5 h; finally adding fucosidase with the mass of 0.015 percent of the algae liquid, and carrying out enzymolysis for 0.5h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain raw algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the biological fertilizer is obtained by mixing the chlorella extract and 5-aminolevulinic acid.
Example 3A biofertilizer
A biological fertilizer comprises 25 mass percent of: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella liquid with the density of 30 hundred million/ml;
(2) adding cellulase 0.08% of the algae solution into the algae solution, and performing enzymolysis at 52-60 deg.C and pH 6.0-6.4 for 1.25 hr; adding pectinase 0.12% of the mass of the algae solution, and performing enzymolysis at 48-56 deg.C and pH 3.5-4.5 for 2 hr; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65 deg.C and pH of 7.5-9.5; finally adding fucosidase with the mass of 0.010% of the algae liquid, and performing enzymolysis for 1h at 48-55 deg.C and pH of 5.8-6.7. Filtering to obtain raw algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the biological fertilizer is obtained by mixing the chlorella extract and 5-aminolevulinic acid.
Example 4A biofertilizer
A biological fertilizer comprises 45: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella solution with density of 30 hundred million/ml;
(2) adding cellulase 0.08% of the algae solution into the algae solution, and performing enzymolysis at 52-60 deg.C and pH 6.0-6.4 for 1.25 hr; adding pectinase 0.12% of the mass of the algae solution, and performing enzymolysis at 48-56 deg.C and pH 3.5-4.5 for 2 hr; adding alkaline protease 0.10% of the algae solution, and performing enzymolysis at 40-65 deg.C and pH 7.5-9.5 for 1 hr; finally adding fucosidase with the mass of 0.010% of the algae liquid, and performing enzymolysis for 1h at 48-55 deg.C and pH of 5.8-6.7. Filtering to obtain raw algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the chlorella extract is mixed with 5-aminolevulinic acid to obtain the biological fertilizer.
Example 5A biofertilizer
A biological fertilizer comprises 50: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella liquid with the density of 30 hundred million/ml;
(2) adding cellulase 0.08% of the algae solution into the algae solution, and performing enzymolysis at 52-60 deg.C and pH 6.0-6.4 for 1.25 hr; adding pectinase 0.12% of the mass of the algae solution, and performing enzymolysis at 48-56 deg.C and pH 3.5-4.5 for 2 hr; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65 deg.C and pH of 7.5-9.5; finally adding fucosidase with the mass of 0.010% of the algae liquid, and performing enzymolysis for 1h at 48-55 deg.C and pH of 5.8-6.7. Filtering to obtain original algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the biological fertilizer is obtained by mixing the chlorella extract and 5-aminolevulinic acid.
Example 6A biofertilizer
A biological fertilizer comprises the following components in a mass ratio of 8: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella liquid with the density of 30 hundred million/ml;
(2) adding cellulase accounting for 0.08% of the mass of the algae solution into the algae solution, and performing enzymolysis for 1.25h at 52-60 deg.C and pH of 6.0-6.4; adding pectinase 0.12% of the mass of the algae solution, and performing enzymolysis at 48-56 deg.C and pH 3.5-4.5 for 2 hr; adding alkaline protease 0.10% of the algae solution, and performing enzymolysis at 40-65 deg.C and pH 7.5-9.5 for 1 hr; finally adding fucosidase with the mass of 0.010% of the algae liquid, and performing enzymolysis for 1h at 48-55 deg.C and pH of 5.8-6.7. Filtering to obtain raw algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the biological fertilizer is obtained by mixing the chlorella extract and 5-aminolevulinic acid.
Example 7A biofertilizer
A biological fertilizer comprises the following components in percentage by mass of 55: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella liquid with the density of 30 hundred million/ml;
(2) adding cellulase 0.08% of the algae solution into the algae solution, and performing enzymolysis at 52-60 deg.C and pH 6.0-6.4 for 1.25 hr; adding pectinase 0.12 wt% of the algae solution, and performing enzymolysis at 48-56 deg.C and pH of 3.5-4.5 for 2 hr; adding alkaline protease 0.10% of the algae solution, and performing enzymolysis at 40-65 deg.C and pH 7.5-9.5 for 1 hr; finally adding fucosidase with the mass of 0.010% of the algae liquid, and performing enzymolysis for 1h at 48-55 deg.C and pH of 5.8-6.7. Filtering to obtain raw algae solution;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, the biological fertilizer is obtained by mixing the chlorella extract and 5-aminolevulinic acid.
Example 8A biofertilizer
A biological fertilizer comprises the following components in percentage by mass of 25: 1 and 5-aminolevulinic acid, wherein the chlorella extract is a commercially available product from belka biotechnology limited, guangzhou.
Specifically, the biological fertilizer is obtained by mixing the chlorella extract and 5-aminolevulinic acid.
Comparative example 1
The preparation method of the single chlorella extract comprises the following steps:
(1) performing high-density heterotrophic culture on chlorella to obtain chlorella solution with density of 30 hundred million/ml;
(2, adding cellulase accounting for 0.08 percent of the mass of the algae liquid into the algae liquid, carrying out enzymolysis for 1.25 hours at the temperature of 52-60 ℃ and the pH value of 6.0-6.4, adding pectinase accounting for 0.12 percent of the mass of the algae liquid, carrying out enzymolysis for 2 hours at the temperature of 48-56 ℃ and the pH value of 3.5-4.5, adding alkaline protease accounting for 0.10 percent of the mass of the algae liquid, carrying out enzymolysis for 1 hour at the temperature of 40-65 ℃ and the pH value of 7.5-9.5, finally adding fucosidase accounting for 0.010 percent of the mass of the algae liquid, carrying out enzymolysis for 1 hour at the temperature of 48-55 ℃ and the pH value of 5.8-6.7, and filtering to obtain a raw algae liquid;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
Comparative example 2
5-aminolevulinic acid alone.
Evaluation of application Effect
First, spraying effect
The bio-fertilizer prepared in examples 1-8 and the single bio-fertilizer of comparative examples 1-2 were diluted in a bio-fertilizer to water ratio of 1:1000, spraying was started after 2 true leaves were grown from tomato seedlings, and then spraying was performed every 7d for 2 months.
2 months later, the plant height, plant dry weight, root length change, leaf area, leaf chlorophyll content, strong seedling index and growth function of the tomato are inspected, and the results are shown in figures 1-7.
Compared with the method before application, after the bio-fertilizer containing the chlorella active extract components prepared in the examples 1-5 is sprayed on tomato plants, the plant height, the root length, the leaf area, the chlorophyll, the seedling strengthening index, the growth function, the plant dry weight and the stem thickness are all improved, which shows that the bio-fertilizer containing the chlorella active extract components obviously promotes the growth of the tomato plants. From the examples before application, comparative examples 1 and 2, only the chlorella active extract-containing component and 5-aminolevulinic acid have a certain growth promoting effect on plants, but the effect is remarkably lower than the plant growth promoting effect after the chlorella active extract and the 5-aminolevulinic acid are mixed, meanwhile, the examples 6, 7 and 8 can find that the chlorella active extract and the 5-aminolevulinic acid which are not conventionally mixed can play the effect of promoting the plant growth, which shows that the chlorella active extract-containing component and the 5-aminolevulinic acid provided by the examples 1 to 5 of the invention can have a synergistic effect, and the effect is remarkably higher than the plant growth promoting effect of a single component.
Second, root irrigation effect
The bio-fertilizer prepared in the examples 1 to 8 and the single bio-fertilizer of the comparative examples 1 to 2 were diluted in the ratio of fertilizer to water of 1:700, and after 2 true leaves of tomato seedlings grew, root irrigation was started, and then root irrigation was performed once every 7 days for 2 months. After 2 months, the plant height, the plant dry weight, the root length change, the leaf area, the leaf chlorophyll content, the strong seedling index and the growth function of the tomato are inspected, and the plant height, the root length, the leaf area, the chlorophyll content, the strong seedling index, the growth function, the plant dry weight and the stem thickness are all improved after the bio-fertilizer containing the chlorella active extract components prepared in the examples 1-5 is sprayed on the tomato plant before application, so that the effect of obviously promoting the growth of the tomato plant can be also realized by adopting root irrigation treatment of the bio-fertilizer.
The present invention is not limited to the above-described preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. The biological fertilizer is characterized by comprising the following components in a mass ratio of 10: 1-50: 1 and 5-aminolevulinic acid.
2. The biofertilizer of claim 1, wherein the mass ratio of chlorella extract to 5-aminolevulinic acid is 15: 1-45: 1.
3. the biofertilizer of claim 1, wherein the preparation method of chlorella extract comprises the steps of:
(1) heterotrophic culture of chlorella to obtain chlorella solution;
(2) carrying out enzymolysis on the algae liquid obtained in the step (1), and filtering to obtain a raw algae liquid;
(3) and (3) spray-drying the prototheca liquid obtained in the step (2) to obtain the chlorella extract.
4. The biological fertilizer according to claim 3, wherein the enzymes used in the enzymatic hydrolysis in step (2) are cellulase, pectinase, alkaline protease and fucosidase.
5. A method for preparing a biological fertilizer according to any one of claims 1 to 4, characterized in that the method comprises: mixing the chlorella extract and the 5-aminolevulinic acid according to the formula amount to obtain the biological fertilizer.
6. Use of a biological fertilizer according to any one of claims 1-4 for promoting plant growth.
7. Use according to claim 6, characterized in that the biofertilizer is to be applied in a manner such that: spraying and/or irrigating roots.
8. Use according to claim 7, characterized in that the dilution ratio of the spray is: and (3) biological fertilizer: water 1: 500-1: 1500.
9. the use according to claim 7, characterized in that the dilution ratio of the irrigation roots is: and (3) biological fertilizer: 1 in water: 300-1: 1000.
10. a biological fertilizer, which comprises the biological fertilizer according to any one of claims 1 to 4 or the biological fertilizer produced according to claim 5 and an auxiliary material.
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| CN103664333A (en) * | 2013-11-12 | 2014-03-26 | 宿迁市设施园艺研究院 | Composition containing 5-ALA and mycose and leaf fertilizer prepared by using composition |
| CN106748195A (en) * | 2016-12-28 | 2017-05-31 | 烟台泓源生物肥料有限公司 | A kind of fertilizer and its preparation technology containing natural plants growth substance |
| CN108821869A (en) * | 2018-08-01 | 2018-11-16 | 四川国光农化股份有限公司 | A kind of fertilizer composition and its application |
| CN109369270A (en) * | 2018-11-26 | 2019-02-22 | 烟台富康生物科技有限公司 | A kind of environment-friendly fertilizer and its preparation process containing functional components of chlorella vulgaris |
| CN113293123A (en) * | 2021-07-15 | 2021-08-24 | 山东深海生物科技股份有限公司 | Application of 5-aminolevulinic acid in promoting growth of chlorella keiskei |
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