CN103664333A - Composition containing 5-ALA and mycose and leaf fertilizer prepared by using composition - Google Patents

Abstract

The invention relates to a composition containing 5-ALA and mycose and a leaf fertilizer prepared by using the composition. The composition for preparing the leaf fertilizer contains 2000-4000 parts by weight of mycose and 0.5-1 part by weight of 5-ALA. According to the leaf fertilizer containing 5-ALA and mycose, the concentration of the mycose is 2-4g/L, and the concentration of the 5-ALA is 0.5-1mg/L. The 5-ALA and the mycose are compounded at specific concentration, and multiple nutrient elements at specific concentration are used to prepare the compound leaf fertilizer. The 5-ALA and the mycose are synergized and can enhance the content of chlorophyll in the blade in comparison with separately using 5-ALA and the mycose, the photosynthesis of multiple crops is promoted, particularly under adverse situation, the stress resistance of crops can be remarkably enhanced, the growth of plants is greatly promoted, and the yield and quality of crops are improved by maintaining the stability of cells, lowering membrane lipid peroxidation and other physiological ways.

Description

The foliage fertilizer of a kind of composition that contains 5-ALA and trehalose and preparation thereof

Technical field

The invention belongs to fertilizer field, be specifically related to the composition of a kind of 5-ALA of containing and trehalose and the foliage fertilizer of preparation thereof.

Background technology

In recent years, research about foliage fertilizer is a lot, product category is abundant, " the seaweeds foliage fertilizer " that " the photosynthetic power agent of 5-ALA " of producing as Na Meite bio tech ltd, Suzhou, " the philharmonic strong amino acid water soluble fertilizer " that Yi An bio tech ltd, Suzhou produces and Qingdao bright moon company produce etc., all there is good market sales volume, for developing eco-agriculture, make larger contribution, obtained good economic benefit and social benefit.But in production application, it is found that this two classes foliage fertilizer all has certain defect, although 5-ALA class foliage fertilizer spraying effect is obvious, but due to cell injury etc., that blade top layer is absorbed is insufficient under the environment stresses such as low temperature, high temperature, salt stress for plant leaf, during high density, even can make crop blade face produce blemish, therefore not reach desirable effect.Though seaweeds foliage fertilizer contains various plants growth substance, trehalose and part nutritive element, spraying effect is better, compares with 5-ALA foliage fertilizer, promoting plant leaf photosynthesis, improves plant dry substance accumulation aspect relatively poor.In sum, though relative other the common foliage fertilizers of above two class foliage fertilizers have good effect, all have some limitations, can not be utilized substantially by plant, to improve plant strain growth and fruit quality etc.

5-ALA is the synthetic precursor substance of plant chlorophyll, can promote chlorophyllous synthetic, improve plant photosynthesis, to improving the multiple resistance such as the anti-low temperature of plant, anti-salt, drought resisting, also there is very significantly effect, and, the applied research of 5-ALA photodynamics on clinical medicine is in recent years very extensive, and the diseases such as treatment tetter and cancer are had to good effect.Trehalose have the good reputation of " sugar of life " in scientific circles; it has magical provide protection to organism; because trehalose can form unique protective membrane at cell surface under the severe environmental conditions such as high temperature, high and cold, high osmotic pressure and arid dehydration; improve the stability of cells of superficial layer; protected protein matter molecule unchangeability inactivation effectively, thereby the vital process of the body that sustains life and biological characteristic.Many severe environment to external world show the species of outstanding degeneration-resistant tolerance, all with in their bodies exist a large amount of trehaloses to have certain relation.Therefore these two kinds of materials are useful and harmless to plant, human body etc., belong to green, environmental protection material.When but trehalose and 5-ALA concentration are too low, effect is all not obvious, the film lipid peroxidation of meeting start quickly blade table confluent monolayer cells after using during excessive concentration, suppress chlorophyllous synthetic, thereby reduced chlorophyll content, affect its plant strain growth and crop yield and quality.There is no at present the relevant report of the composite foliage fertilizer of 5-ALA and trehalose.

Summary of the invention

The object of the invention is to the above-mentioned defect for prior art, the composition of a kind of 5-ALA of containing and trehalose is provided.

Another object of the present invention is to provide the foliage fertilizer of being prepared by said composition.

Object of the present invention can be achieved through the following technical solutions:

For the preparation of a composition for foliage fertilizer, contain trehalose 2000～4000 weight parts and 5-ALA0.5～1 weight part.

Described composition, preferably also comprises following nutritive substance: KNO ₃600～620 weight parts, NH ₄h ₂pO ₄110～120 weight parts, MgSO ₄7H ₂o95～105 weight part, NaFe-EDTA18～23 weight part, H ₃bO ₃2.5～3.0 weight parts, MnSO ₄h ₂o2.0～2.2 weight part, ZnSO ₄7H ₂o0.20～0.25 weight part, CuSO ₄5H ₂o0.05～0.10 weight part, (NH ₄) ₆moO ₂₄4H ₂o0.01～0.04 weight part.

In described composition, nutritive substance is further preferably as follows consumption: KNO ₃607 weight parts, NH ₄h ₂pO ₄115 weight parts, MgSO ₄7H ₂o100 weight part, NaFe-EDTA20 weight part, H ₃bO ₃2.86 weight parts, MnSO ₄h ₂o2.13 weight part, ZnSO ₄7H ₂o0.22 weight part, CuSO ₄5H ₂o0.08 weight part, (NH ₄) ₆moO ₂₄4H ₂o0.02 weight part.

The application of composition of the present invention in preparing foliage fertilizer.

A 5-ALA trehalose composite foliage fertilizer, contains water and composition of the present invention, before spraying by described composition dissolves in described water, making trehalose concentration is 2g/L～4g/L, the solution that the concentration of 5-ALA is 0.5mg/L～1mg/L.

As a kind of optimal way of the present invention, 5-ALA trehalose composite foliage fertilizer before spraying by described composition dissolves in described water, make trehalose 2g/L～4g/L, 5-ALA0.5mg/L～1mg/L, KNO ₃600～620mg/L, NH ₄h ₂pO ₄110～120mg/L, MgSO ₄7H ₂o95～105mg/L, NaFe-EDTA18～23mg/L, H ₃bO ₃2.5～3.0mg/L, MnSO ₄h ₂o2.0～2.2mg/L, ZnSO ₄7H ₂o0.20～0.25mg/L, CuSO ₄5H ₂o0.05～0.10mg/L, (NH ₄) ₆moO ₂₄4H ₂the solution of O0.01～0.04mg/L.

As the most preferred mode of the present invention, 5-ALA trehalose composite foliage fertilizer before spraying by described composition dissolves in described water, make trehalose 4g/L, 5-ALA1mg/L, KNO ₃607mg/L, NH ₄h ₂pO ₄115mg/L, MgSO ₄7H ₂o100mg/L, NaFe-EDTA20mg/L, H ₃bO ₃2.86mg/L, MnSO ₄h ₂o2.13mg/L, ZnSO ₄7H ₂o0.22mg/L, CuSO ₄5H ₂o0.08mg/L, (NH ₄) ₆moO ₂₄4H ₂the solution of O0.02mg/L.

Foliage fertilizer of the present invention is recommended morning or is carried out foliage-spray at dusk.

The preparation method of described composition, comprises following steps:

(1) get KNO ₃, NH ₄h ₂pO ₄and MgSO ₄7H ₂o, through metering, is mixed and made into A component after pulverizing respectively;

(2) get NaFe-EDTA, H ₃bO ₃, MnSO ₄h ₂o, ZnSO ₄7H ₂o, CuSO ₄5H ₂o and (NH ₄) ₆moO ₂₄4H ₂o pulverize respectively after metering and mix with A component after make B component;

(3) get 5-ALA, trehalose and be mixed and made into C component with B component after metering;

(4) C component is pulverized and be get final product.

On this basis, in spraying, frontly become respective concentration to make foliage fertilizer of the present invention described composition thin up.

Beneficial effect:

The present invention is composite with specific proportioning by 5-ALA and trehalose, and the nutritive element that is aided with multiple certain concentration made the composition for the preparation of composite foliage fertilizer, and further grope on this basis to have determined the optimum amount of each composition in foliage fertilizer in composition.The trehalose concentration that experiment is found is 2g/L～4g/L, when the concentration of 5-ALA is 0.5mg/L～1mg/L, the two can work in coordination with onset, than independent use 5-ALA and trehalose, can significantly improve blade Determination of Chlorophyll content, promote multiple kinds of crops photosynthesis, particularly under adverse circumstance can by maintain cell stability, reduce the multiple physiological pathway such as film lipid peroxidation, significantly strengthen the resistance of crop, thereby significantly promote robust plant growth, improve crop yield and quality.

Embodiment

Embodiment 1

The first step: get KNO ₃9.105g, NH ₄h ₂pO ₄1.725g and MgSO ₄7H ₂o1.5g, through metering, is mixed and made into A component after pulverizing respectively.

Second step: get NaFe-EDTA0.3g, H ₃bO ₃0.0429g, MnSO ₄h ₂o0.03195g, ZnSO ₄7H ₂o0.0033g, CuSO ₄5H ₂o0.0012g and (NH ₄) ₆moO ₂₄4H ₂o0.0003g pulverize respectively after metering and mix with A component after make B component.

The 3rd step: get 5-ALA0.015g, trehalose 60g and B component and be mixed and made into C component.

The 4th step: C component is pulverized and made the composition for the preparation of foliage fertilizer.

The 5th step: spray and front composition is diluted and is prepared into trehalose 4g/L, 5-ALA1mg/L, KNO with 15L water ₃607mg/L, NH ₄h ₂pO ₄115mg/L, MgSO ₄7H ₂o100mg/L, NaFe-EDTA20mg/L, H ₃bO ₃2.86mg/L, MnSO ₄h ₂o2.13mg/L, ZnSO ₄7H ₂o0.22mg/L, CuSO ₄5H ₂o0.08mg/L, (NH ₄) ₆moO ₂₄4H ₂the foliage fertilizer solution of O0.02mg/L.

In production, generally carry out in the morning or at dusk foliage-spray.

Embodiment 2

Take cucumber as examination material, adopt matrix hole plate seedling growth, average culture temperature is daytime/night: 28 ℃/19 ℃, after appearring in cucumber seedling, the 3rd true leaf start to spray each test group foliage fertilizer in the dusk, within every 5 days, spray once, spray altogether 3 times, processing finish 5 days afterwards survey respectively process growth increment.Each test of the present invention is processed as described in Table 1.'+' represents to be added with nutritive substance, and '-' represents not add nutritive substance.Nutritive substance is KNO for the concentration in examination group at each ₃607mg/L, NH ₄h ₂pO ₄115mg/L, MgSO ₄7H ₂o100mg/L, NaFe-EDTA20mg/L, H ₃bO ₃2.86mg/L, MnSO ₄h ₂o2.13mg/L, ZnSO ₄7H ₂o0.22mg/L, CuSO ₄5H ₂o0.08mg/L, (NH ₄) ₆moO ₂₄4H ₂o0.02mg/L.Each preparation method for examination group foliage fertilizer is with embodiment 1, and the 5-ALA only adding is different with content of trehalose.

Table 1: test is processed

Table 2: each processes the impact on cucumber plant growth

Table 3: trehalose is processed the impact on cucumber plant growth

Note: this table by the seaweed nutritive liquid processing of different concns with contrast and aqueous trehalose solution compares.In same column different letter representation process the same period between in 0.05 level significant difference.

Table 4:5-ALA processes the impact on cucumber plant growth

Note: this table by the 5-ALA nutritive medium processing of different concns with contrast and the 5-ALA aqueous solution compares.In same column different letter representation process the same period between in 0.05 level significant difference.

Table 5: trehalose and 5-ALA are mixed and process the impact on cucumber plant growth

Note: this table by trehalose and 5-ALA be mixed preferably processing with contrast and high density is mixed to process and compares.In same column different letter representation process the same period between in 0.05 level significant difference.Data by table 3 can be found out, spray merely after the trehalose nutritive medium of lower concentration, growth increment contrasts and being all significantly increased of simple spray nutritious liquor, but the increasing along with trehalose concentration, each increment low concentration of plant is processed be first raise after reduction trend, wherein relatively better with the trehalose nutritive medium spraying effect of 2～4g/L, and the trehalose nutritive medium spraying effect of 4g/L is the suitableeest, the plant gross dry weight of this processing contrasts and has improved 30.46%, wherein from T5 and T38, can find out, it is better than spraying merely aqueous trehalose solution effect to spray trehalose nutritive medium.

Data by table 4 can be found out, spray merely after the 5-ALA nutritive medium of lower concentration, growth increment contrasts and being also all significantly increased of simple spray nutritious liquor, but the increasing along with 5-ALA concentration, each increment low concentration of plant is processed be first raise after reduction trend, wherein relatively the suitableeest with 1mg/L ALA nutritive medium spraying effect, the plant gross dry weight of this processing contrasts and has improved 38.12%, by T12 and T40, can be found out, spray ALA nutritive medium better than spraying merely ALA aqueous solution effect.

Data by table 5 can find out, after the trehalose of nutritive medium and different ratios and ALA are mixed, spray cucumber leaves, and the be mixed growth increment of solution-treated of lower concentration is significantly better than contrast; But along with the concentration of trehalose and ALA strengthens, what its spraying effect low concentration was processed decreases.In general, the effect spraying with T24 is processed best compared with other, and compared with the control for extremely remarkable, its plant gross dry weight has improved 96.51%; Compared with the trehalose nutritive medium of 4g/L process, the ALA nutritive medium of 1mg/L is processed and the trehalose of 4g/L and the ALA of 1mg/L are mixed, and aqueous solution processing is also all significantly increased, its plant gross dry weight has improved respectively 50.63%, 42.27% and 9.1%.Show that the trehalose and the 5-ALA that spray suitable proportion are mixed after nutritive medium, the two has produced the effect of synergy, can obviously promote plant strain growth, particularly by improving the approach such as plant photosynthesis, increase the accumulation of plant dry-matteies, but can suppress plant strain growth can show that working concentration is excessive by processing such as T37 time.

In addition, compared with the control, the plant leaf chlorophyll content that the ALA of lower concentration and trehalose nutritive medium are processed is improved, after the solution-treated of high density, decrease, may be the ALA of lower concentration and trehalose nutritive medium can be by maintaining cell stability, promote chlorophyllous synthetic, thereby improve chlorophyll content, and the ALA of high density and trehalose mix use after the film lipid peroxidation of start quickly blade table confluent monolayer cells, suppress chlorophyllous synthetic, thereby reduced chlorophyll content.

Claims (6)

1. for the preparation of a composition for foliage fertilizer, it is characterized in that containing trehalose 2000～4000 weight parts and 5-ALA0.5～1 weight part.

2. composition according to claim 1, is characterized in that also comprising following nutritive substance: KNO ₃600～620 weight parts, NH ₄h ₂pO ₄110～120 weight parts, MgSO ₄7H ₂o95～105 weight part, NaFe-EDTA18～23 weight part, H ₃bO ₃2.5～3.0 weight parts, MnSO ₄h ₂o2.0～2.2 weight part, ZnSO ₄7H ₂o0.20～0.25 weight part, CuSO ₄5H ₂o0.05～0.10 weight part, (NH ₄) ₆moO ₂₄4H ₂o0.01～0.04 weight part.

3. composition according to claim 2, is characterized in that also comprising following nutritive substance: KNO ₃607 weight parts, NH ₄h ₂pO ₄115 weight parts, MgSO ₄7H ₂o100 weight part, NaFe-EDTA20 weight part, H ₃bO ₃2.86 weight parts, MnSO ₄h ₂o2.13 weight part, ZnSO ₄7H ₂o0.22 weight part, CuSO ₄5H ₂o0.08 weight part, (NH ₄) ₆moO ₂₄4H ₂o0.02 weight part.

4. the application of the composition described in any one in preparing foliage fertilizer in claim 1～3.

5. a 5-ALA trehalose composite foliage fertilizer, it is characterized in that containing the composition described in any one in water and claim 1～3, before spraying by described composition dissolves in described water, making trehalose concentration is 2g/L～4g/L, the solution that 5-ALA concentration is 0.5mg/L～1mg/L.

6. the preparation method of the composition described in claim 2 or 3, is characterized in that comprising following steps:

(1) get KNO ₃, NH ₄h ₂pO ₄and MgSO ₄7H ₂o, through metering, is mixed and made into A component after pulverizing respectively;

(2) get NaFe-EDTA, H ₃bO ₃, MnSO ₄h ₂o, ZnSO ₄7H ₂o, CuSO ₄5H ₂o and (NH ₄) ₆moO ₂₄4H ₂o pulverize respectively after metering and mix with A component after make B component;

(3) get 5-ALA, trehalose and B component and be mixed and made into C component;

(4) C component is pulverized and made the described composition for the preparation of foliage fertilizer.