CN114920598B - Biological fertilizer and preparation method and application thereof - Google Patents
Biological fertilizer and preparation method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a biological fertilizer and a preparation method and application thereof, wherein the biological fertilizer comprises the following components in percentage by mass: 1-50:1 and 5-aminolevulinic acid, said use being the use of biofertilizer in promoting plant growth. The chlorella extract provided by the invention can effectively extract and retain bioactive components in chlorella, and improves the utilization rate of the effective components of chlorella. Meanwhile, the 5-aminolevulinic acid and chlorella extract are selected and compounded, so that the promoting effect on plant growth can be obviously improved, seed germination can be effectively promoted, root vigor is increased, photosynthesis of leaves is obviously improved, the yield of crops is obviously improved, and a synergistic effect is exerted. In addition, the biological fertilizer has the advantages of few components, simple preparation process, low cost, simple use method, good effect of promoting plant growth and easy popularization and application.
Description
Technical Field
The invention belongs to the technical field of biofertilizers, relates to a biofertilizer and a preparation method and application thereof, and in particular relates to a biofertilizer containing chlorella and a preparation method and application thereof.
Background
The fertilizer makes an important contribution to improving the crop yield, but with the continuous increase of the fertilizer usage amount, the quality safety and environmental safety problems of the fertilizer facing China are increasingly prominent. Especially, in recent years, with the excessive use of fertilizers, environmental pollution and ecological deterioration are caused, the phenomena of soil structure damage, organic matter content reduction and the like are caused, and a series of serious ecological environment problems such as eutrophication of rivers and lakes, quality reduction of agricultural products and the like are caused in partial areas. The reason for this is not only the problem of the scientific fertilizer, but also the quality problem of the fertilizer. The quality safety of the fertilizer severely restricts the sustainable development of agriculture, and threatens the physical and mental health of people directly or indirectly. Therefore, the environment-friendly fertilizer is greatly popularized from the viewpoint of the quality safety of the fertilizer, and the environmental pollution is effectively prevented.
Chlorella is a common unicellular green algae, is widely distributed in nature, is mostly in fresh water areas, has large biomass, can utilize light energy to autotrophy, can also utilize organic carbon sources to grow and reproduce under heterotrophic conditions, and has 15 kinds of chlorella in the world at present and hundreds of varieties. The content of the crude protein of the chlorella is particularly high and can reach more than 50%, especially the chlorella pyrenoidosa is the highest, and the content of the crude protein is much higher than that of the crude protein (40%) of the soybean, and the protein quality is much better. Among the essential amino acids, lysine, arginine, leucine and valine are high in content, phenylalanine, isoleucine and threonine are high in content, histidine is low in content, and methionine and tryptophan are low in content. Also contains abundant physiologically active ingredients such as: growth Factors (CGF), beta-carotene, chlorophyll, vitamins, trace elements, polysaccharide phycocyanin, unsaturated fatty acids, etc. Because the chlorella has higher nutrient content and has the health care function, the chlorella is developed for protein source feed, food, beverage and medical products.
The 5-aminolevulinic acid is taken as a key precursor substance required by chlorophyll synthesis, can obviously improve photosynthesis of leaves, obviously improve the yield of crops, and particularly induces various crops to generate tolerance to various abiotic stresses under adverse conditions such as low temperature, drought, saline alkali and the like. The cucumber seedlings are treated by adding a small amount of 5-aminolevulinic acid, the antioxidant enzyme activity of plants is obviously improved, and the promotion effect is weakened along with the increase of the concentration. The 5-aminolevulinic acid is also a novel photoactivation pesticide, can be used as a plurality of agents such as plant physiological response regulator, green herbicide, pesticide and the like, and has wide application in the agricultural field.
At present, the development and patent application of chlorella biofertilizer are mainly focused on the aspects of mixing chlorella with biological organic matters such as chemical fertilizers, bacillus, livestock manure, straw and the like, the components of the mixed fertilizers are complex, the steps are complicated, the stability of biological activity is to be questioned, and the search is carried out, so that the biofertilizer taking chlorella extract and 5-aminolevulinic acid as active components has not been found yet.
Disclosure of Invention
In view of the above, the invention provides a biological fertilizer, a preparation method and application thereof, wherein the biological fertilizer takes chlorella extract as a main raw material, so that the problems of unstable activity and incomplete exertion of active ingredients caused by directly utilizing chlorella as the raw material are avoided, and the biological fertilizer has remarkable plant growth promoting effect.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a biological fertilizer comprises the following components in percentage by mass: 1-50:1 and 5-aminolevulinic acid.
Preferably, the mass ratio of the chlorella extract to the 5-aminolevulinic acid is 15:1-45:1.
preferably, the preparation method of the chlorella extract comprises the following steps:
(1) Heterotrophic culture of Chlorella to obtain algae liquid;
(2) Carrying out enzymolysis and filtration on the algae liquid obtained in the step (1) to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Further preferably, the enzymes used in the enzymolysis in step (2) are cellulases, pectinases, alkaline proteases and fucosidases.
Further preferably, the enzymolysis in step (2) is: adding cellulase into the algae liquid, and performing enzymolysis for 1-1.5h at 52-60deg.C and pH value of 6.0-6.4; adding pectase, and performing enzymolysis at 48-56 deg.C and pH of 3.5-4.5 for 1-2 hr; adding alkaline protease, and performing enzymolysis at 40-65deg.C and pH of 7.5-9.5 for 0.5-1 hr; finally adding fucosidase, and performing enzymolysis at 48-55deg.C and pH of 5.8-6.7 for 0.5-1.5 hr.
Further preferably, the addition amount of the cellulase is 0.05-0.1% of the mass of the algae liquid, the addition amount of the pectase is 0.1-0.15% of the mass of the algae liquid, the addition amount of the alkaline protease is 0.08-0.12% of the mass of the algae liquid, and the addition amount of the fucosidase is 0.005-0.015% of the mass of the algae liquid.
The invention also provides a preparation method of the biological fertilizer, which comprises the following steps: mixing chlorella extract and 5-aminolevulinic acid according to the formula amount to obtain the biological fertilizer.
The invention also provides application of the biological fertilizer in promoting plant growth.
Preferably, the bio-fertilizer is applied in the following manner: spraying and/or root irrigation.
Further preferably, the dilution ratio of the spray is: biological fertilizer: water = 1:500-1:1500.
further preferably, the dilution ratio of the root irrigation is: biological fertilizer: water = 1:300-1:1000.
the invention also provides a biological fertilizer, which comprises the biological fertilizer or the biological fertilizer prepared by the preparation method and auxiliary materials.
The beneficial effects of the invention are as follows:
in the process of producing seaweed-derived biostimulant by using algae, on one hand, algae are digested, so that cell walls are completely broken as much as possible, and then the contents in cells are released, and small molecular substances which are degraded by macromolecular substances in the cells and are easy to be absorbed and utilized are obtained; on the other hand, the phytohormone therein is well reserved. In the early seaweed-derived biostimulant production process, people attach great importance to the extraction of nutrients such as polysaccharide, mineral elements and trace elements, and a high-temperature and high-pressure extraction mode is generally adopted, so that plant hormones contained in seaweed are destroyed, and the produced fertilizer has no obvious effect on the growth promotion of crops.
The chlorella extract prepared by the invention can effectively extract and retain the bioactive components in chlorella, and improves the utilization rate of the effective components of chlorella.
The invention selects the 5-aminolevulinic acid and chlorella extract for compounding, can obviously improve the promoting effect on plant growth, effectively promote seed germination, increase root system activity, obviously improve photosynthesis of leaves, obviously improve the yield of crops, and play a role in synergy.
The biological fertilizer has the advantages of few components, simple preparation process, low cost, simple use method, good effect of promoting plant growth and easy popularization and application.
Drawings
FIG. 1 is a statistical graph of plant height increase after spray treatment;
FIG. 2 is a statistical graph of plant dry weight variation after spray treatment;
FIG. 3 is a statistical graph of root length variation after spray treatment;
FIG. 4 is a statistical graph of leaf area increase after spray treatment;
FIG. 5 is a statistical graph of chlorophyll content variation after spray treatment;
FIG. 6 is a statistical graph of the index change of strong seedlings after spraying treatment;
fig. 7 is a statistical graph of the change in growth function after spray treatment.
Detailed Description
In order to make the technical means, the creation features, the achievement of the purpose and the effect of the present invention easy to understand, the present invention will be further elucidated with reference to the specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all of them. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the invention. In the following examples, unless otherwise specified, the methods of operation used were conventional, the equipment used were conventional, and the raw materials used were commercially available, wherein the purity of 5-aminolevulinic acid was not less than 98%.
Example 1A biofertilizer
A biological fertilizer comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid.
The preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase accounting for 0.05% of the mass of the algae liquid into the algae liquid, and performing enzymolysis for 1.5h at the temperature of 52-60 ℃ and the pH value of 6.0-6.4; adding pectase with the mass of 0.1% of that of the algae liquid, and performing enzymolysis for 2 hours at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with 0.08% of the algae liquid mass, and performing enzymolysis for 1h at 40-65deg.C and pH of 7.5-9.5; finally adding fucosidase with the mass of 0.005% of the algae liquid, and performing enzymolysis for 1.5h at 48-55deg.C and pH of 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 2A biofertilizer
A biological fertilizer comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase with the mass of 0.1% of the algae liquid into the algae liquid, and performing enzymolysis for 1h at the temperature of 52-60 ℃ and the pH value of 6.0-6.4; adding pectase with the mass of 0.15% of that of the algae liquid, and performing enzymolysis for 1h at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with the mass of 0.12% of that of the algae liquid, and performing enzymolysis for 0.5h at the temperature of 40-65 ℃ and the pH value of 7.5-9.5; finally, adding fucosidase accounting for 0.015 percent of the mass of the algae liquid, and carrying out enzymolysis for 0.5h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 3A biofertilizer
A biological fertilizer, which comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase accounting for 0.08% of the mass of the algae liquid into the algae liquid, and performing enzymolysis for 1.25h at 52-60 ℃ and pH value of 6.0-6.4; adding pectase with the mass of 0.12% of that of the algae liquid, and performing enzymolysis for 2 hours at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65deg.C and pH of 7.5-9.5; finally, adding fucosidase with the mass of 0.010% of the algae liquid, and carrying out enzymolysis for 1h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 4A biofertilizer
A biological fertilizer comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase accounting for 0.08% of the mass of the algae liquid into the algae liquid, and performing enzymolysis for 1.25h at 52-60 ℃ and pH value of 6.0-6.4; adding pectase with the mass of 0.12% of that of the algae liquid, and performing enzymolysis for 2 hours at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65deg.C and pH of 7.5-9.5; finally, adding fucosidase with the mass of 0.010% of the algae liquid, and carrying out enzymolysis for 1h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 5A biofertilizer
A biological fertilizer, which comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase accounting for 0.08% of the mass of the algae liquid into the algae liquid, and performing enzymolysis for 1.25h at 52-60 ℃ and pH value of 6.0-6.4; adding pectase with the mass of 0.12% of that of the algae liquid, and performing enzymolysis for 2 hours at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65deg.C and pH of 7.5-9.5; finally, adding fucosidase with the mass of 0.010% of the algae liquid, and carrying out enzymolysis for 1h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 6A biofertilizer
A biological fertilizer comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase accounting for 0.08% of the mass of the algae liquid into the algae liquid, and performing enzymolysis for 1.25h at 52-60 ℃ and pH value of 6.0-6.4; adding pectase with the mass of 0.12% of that of the algae liquid, and performing enzymolysis for 2 hours at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65deg.C and pH of 7.5-9.5; finally, adding fucosidase with the mass of 0.010% of the algae liquid, and carrying out enzymolysis for 1h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 7A biofertilizer
A biological fertilizer comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid,
the preparation method of the chlorella extract comprises the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2) Adding cellulase accounting for 0.08% of the mass of the algae liquid into the algae liquid, and performing enzymolysis for 1.25h at 52-60 ℃ and pH value of 6.0-6.4; adding pectase with the mass of 0.12% of that of the algae liquid, and performing enzymolysis for 2 hours at 48-56 ℃ and pH value of 3.5-4.5; adding alkaline protease with the mass of 0.10% of the algae liquid, and performing enzymolysis for 1h at 40-65deg.C and pH of 7.5-9.5; finally, adding fucosidase with the mass of 0.010% of the algae liquid, and carrying out enzymolysis for 1h under the conditions that the temperature is 48-55 ℃ and the pH value is 5.8-6.7. Filtering to obtain a raw algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Example 8A biofertilizer
A biological fertilizer, which comprises the following components in percentage by mass: 1 and 5-aminolevulinic acid, wherein the chlorella extract is a commercially available product from bellka biotechnology, inc.
Specifically, mixing the chlorella extract and 5-aminolevulinic acid to obtain the biological fertilizer.
Comparative example 1
A single chlorella extract is prepared by the following steps:
(1) Performing high-density heterotrophic culture on the chlorella to obtain an algae liquid with the chlorella density of 30 hundred million/ml;
(2, adding cellulase with the mass of 0.08% of the algae liquid into the algae liquid, carrying out enzymolysis for 1.25 hours at the temperature of 52-60 ℃ and the pH value of 6.0-6.4, adding pectase with the mass of 0.12% of the algae liquid, carrying out enzymolysis for 2 hours at the temperature of 48-56 ℃ and the pH value of 3.5-4.5, adding alkaline protease with the mass of 0.10% of the algae liquid, carrying out enzymolysis for 1 hour at the temperature of 40-65 ℃ and the pH value of 7.5-9.5, and finally adding fucosidase with the mass of 0.010% of the algae liquid, carrying out enzymolysis for 1 hour at the temperature of 48-55 ℃ and the pH value of 5.8-6.7, and filtering to obtain the original algae liquid;
(3) And (3) spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract.
Comparative example 2
Single 5-aminolevulinic acid.
Evaluation of application Effect
1. Spray effect
The biofertilizers prepared in examples 1-8 and the single biofertilizer of comparative examples 1-2 were diluted in a ratio of 1:1000 of biofertilizer to water, spraying was started after 2 true leaves of tomato seedlings were grown, and then spraying was performed every 7d, with a spraying period of 2 months.
After 2 months the tomato plant height, plant dry weight, root length variation, leaf area, leaf chlorophyll content, seedling strengthening index and growth function were examined and the results are shown in fig. 1-7.
Compared with the biological fertilizer containing the chlorella active extract component prepared in the examples 1-5 before application, after the biological fertilizer is sprayed on tomato plants, the plant height, root length, leaf area, chlorophyll, seedling strengthening index, growth function, plant dry weight and stem thickness are all improved, which shows that the biological fertilizer containing the chlorella active extract component obviously promotes the growth of tomato plants. From the examples before application, comparative example 1 and comparative example 2, only the chlorella-containing active extract component and 5-aminolevulinic acid have a certain growth promoting effect on plants, but the effect is obviously lower than the plant growth promoting effect after the chlorella-containing active extract component and 5-aminolevulinic acid component are mixed, meanwhile, the effects of promoting plant growth can be found by the examples 6, 7 and 8, and the effects of the chlorella-containing active extract and 5-aminolevulinic acid component can not be realized by the conventional mixing of the chlorella-containing active extract and 5-aminolevulinic acid, so that the synergistic effect of the chlorella-containing active extract and 5-aminolevulinic acid provided by the examples 1-5 can be obviously higher than the plant growth promoting effect of the single component.
2. Root irrigation effect
The biofertilizer prepared in examples 1-8 and the single biofertilizer prepared in comparative examples 1-2 were diluted in the ratio of fertilizer to water of 1:700, root irrigation was started after 2 true leaves were grown from tomato seedlings, and root irrigation was performed once every 7d, with a root irrigation period of 2 months. After 2 months, the plant height, plant dry weight, root length change, leaf area, leaf chlorophyll content, seedling strengthening index and growth function of the tomatoes are examined, and the biological fertilizer prepared in the examples 1-5 is also found to have improved plant height, root length, leaf area, chlorophyll, seedling strengthening index, growth function, plant dry weight and stem thickness compared with the biological fertilizer prepared in the examples before application and containing chlorella active extract components after spraying on tomato plants, which shows that the biological fertilizer can also have obvious effect of promoting the growth of tomato plants by root irrigation treatment.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (8)
1. A biological fertilizer, characterized by comprising the following components in percentage by mass: 1-50:1 and 5-aminolevulinic acid;
the preparation method of the chlorella extract comprises the following steps:
(1) Heterotrophic culture of Chlorella to obtain algae liquid;
(2) Carrying out enzymolysis and filtration on the algae liquid obtained in the step (1) to obtain a raw algae liquid;
(3) Spray drying the raw algae liquid obtained in the step (2) to obtain the chlorella extract;
the enzymolysis in the step (2) is as follows: adding cellulase into the algae liquid, and performing enzymolysis for 1-1.5h at 52-60deg.C and pH value of 6.0-6.4; adding pectase, and performing enzymolysis at 48-56 deg.C and pH of 3.5-4.5 for 1-2 hr; adding alkaline protease, and performing enzymolysis at 40-65deg.C and pH of 7.5-9.5 for 0.5-1 hr; finally adding fucosidase, and performing enzymolysis for 0.5-1.5h at 48-55deg.C and pH of 5.8-6.7;
the addition amount of the cellulase is 0.05-0.1% of the mass of the algae liquid, the addition amount of the pectase is 0.1-0.15% of the mass of the algae liquid, the addition amount of the alkaline protease is 0.08-0.12% of the mass of the algae liquid, and the addition amount of the fucosidase is 0.005-0.015% of the mass of the algae liquid.
2. The biofertilizer of claim 1, wherein the mass ratio of chlorella extract to 5-aminolevulinic acid is 15:1-45:1.
3. a method for preparing the biofertilizer of any of claims 1-2, characterized in that the method is: mixing chlorella extract and 5-aminolevulinic acid according to the formula amount to obtain the biological fertilizer.
4. Use of a biofertilizer according to any of claims 1-2 for promoting plant growth, said plant being tomato.
5. The use according to claim 4, wherein the biofertilizer is applied in the following manner: spraying and/or root irrigation.
6. The use according to claim 5, wherein the dilution ratio of the spray is: biological fertilizer: water = 1:500-1:1500.
7. the use according to claim 5, wherein the root irrigation dilution ratio is: biological fertilizer: water = 1:300-1:1000.
8. a biofertilizer, characterized by comprising the biofertilizer of any one of claims 1-2 or the biofertilizer prepared in claim 3 and auxiliary materials.
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| CN101885736A (en) * | 2004-03-30 | 2010-11-17 | 克斯莫石油株式会社 | 5-aminolevulinic acid, Its Preparation Method And Use |
| CN106748195A (en) * | 2016-12-28 | 2017-05-31 | 烟台泓源生物肥料有限公司 | A kind of fertilizer and its preparation technology containing natural plants growth substance |
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| JP2613136B2 (en) * | 1991-05-14 | 1997-05-21 | 株式会社コスモ総合研究所 | Plant growth promoter |
| JP5909332B2 (en) * | 2010-07-05 | 2016-04-26 | 株式会社誠和 | 5-aminolevulinic acid adjuvant and method of use thereof |
| CN103664333B (en) * | 2013-11-12 | 2015-03-04 | 宿迁市设施园艺研究院 | Composition containing 5-ALA and mycose and leaf fertilizer prepared by using composition |
| CN108821869A (en) * | 2018-08-01 | 2018-11-16 | 四川国光农化股份有限公司 | A kind of fertilizer composition and its application |
| CN109369270A (en) * | 2018-11-26 | 2019-02-22 | 烟台富康生物科技有限公司 | A kind of environment-friendly fertilizer and its preparation process containing functional components of chlorella vulgaris |
| CN113293123B (en) * | 2021-07-15 | 2023-06-16 | 山东深海生物科技股份有限公司 | Application of 5-aminolevulinic acid in promoting growth of chlorella kei |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101885736A (en) * | 2004-03-30 | 2010-11-17 | 克斯莫石油株式会社 | 5-aminolevulinic acid, Its Preparation Method And Use |
| CN106748195A (en) * | 2016-12-28 | 2017-05-31 | 烟台泓源生物肥料有限公司 | A kind of fertilizer and its preparation technology containing natural plants growth substance |
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