CN114886015A - Method for regulating ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite - Google Patents
Method for regulating ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite Download PDFInfo
- Publication number
- CN114886015A CN114886015A CN202210575999.7A CN202210575999A CN114886015A CN 114886015 A CN114886015 A CN 114886015A CN 202210575999 A CN202210575999 A CN 202210575999A CN 114886015 A CN114886015 A CN 114886015A
- Authority
- CN
- China
- Prior art keywords
- silage
- sweet sorghum
- pyrosulfite
- flora
- saline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for adjusting the ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite, relates to the field of silage, and particularly relates to a silage making method for adjusting the ecology of the flora in sweet sorghum silage. The invention aims to solve the technical problem of low quality of the existing sweet sorghum silage. The method comprises the following steps: crushing and preparing a pyrosulfite solution; filling the filiform crushed aggregates into a shading bag, and exhausting air; storing in shade and dark place. According to the invention, the pyrosulfite solution with a certain concentration is added into the sweet sorghum silage to adjust the distribution of microbial flora, reduce the metabolic activity of non-lactic acid bacteria, avoid the improper consumption of sugar, adjust the activity of lactic acid bacteria in the silage, so that more lactic acid is accumulated in the sweet sorghum silage as early as possible, the breeding of other mixed bacteria or harmful bacteria is inhibited, and the quality of the sweet sorghum silage is improved. The method is used for ensiling the sweet sorghum.
Description
Technical Field
The invention relates to the field of silage, in particular to a silage making method for adjusting the ecology of flora in sweet sorghum silage.
Background
The sweet sorghum is a recognized high-energy crop and has the characteristics of drought resistance and excellent salt and alkali resistance. The method can be widely planted in marginal barren soil such as saline soil, saline soil and the like, is one of the most suitable energy crops, can be planted in saline-alkali soil in a wide range, does not compete for agricultural cultivated land with crops such as corn and the like, and generates certain economic value. The biological yield of the sweet sorghum is high, the sweet sorghum is annual crop in the north and can be harvested twice a year in the south, but because the sugar content in the sweet sorghum is higher and can be comparable with that of sugarcane, a proper storage mode is needed before utilization so as to reduce the loss of the internal sugar content in the transportation process. The ensiling is a storage mode which can be used for storing a large amount of substances at low cost, and can reserve sugar and other available resources in the materials to the maximum extent through proper ensiling conditions. Lactic acid is generated through anaerobic fermentation, and the pH value of the material is reduced, so that the propagation of putrefying bacteria is inhibited, and internal nutrients are protected. Due to the self-growing growth habit and biochemical characteristics of the sweet sorghum, the biomass of the sweet sorghum is about three times of that of corn, and the stalks are rich in sugar and are excellent microbial fermentation raw materials. Researches show that the quality of the sweet sorghum silage is not inferior to that of corn silage, and the sweet sorghum silage is concerned because of simple planting mode, high yield and low planting cost.
In the silage preparation process, the growth condition, the storage temperature, the moisture content of the material slag and the anaerobic degree of the lactic acid bacteria are crucial to the silage quality. Sweet sorghum reaps the back, and the surface adheres to multiple microorganism like yeast, mould etc. and the growth advantage of key lactic acid bacteria in ensiling in the early stage of envelope is not obvious, then leads to in the sealed back of sweet sorghum, the phenomenon of other dominant floras activities can appear in ensiling earlier stage, leads to the loss of sugar in sweet sorghum, and along with the extension of ensiling time, lactic acid bacteria abundance risees gradually, and lactic acid accumulation, silage pH value reduces, forms natural antibacterial environment. However, the activity of the mixed bacteria at the early stage has great influence on the quality of the silage, such as lactic acid content, color and smell, so that a bacteriostatic agent, such as organic acid and sulfur dioxide, can be added during bagging to inhibit the growth of the mixed bacteria so as to prolong the freshness of the materials or a lactic acid bacteria agent to improve the relative abundance of lactic acid bacteria. The storage temperature is too low or too high, which is not beneficial to the quality of silage, the temperature is lower, the metabolism of lactic acid bacteria is slow, and the environment with low pH value can be formed for a longer time. At too high a temperature, microbial metabolism is intense, and although a low pH environment is formed earlier, at high temperatures, protein quality is affected, vitamins are destroyed, and even odor and putrefaction occur. The moisture of the material residues is also the same, the rotting bacteria are easy to breed when the moisture of the raw materials is too high, the material residues are difficult to compress when the moisture is too low, and the residual air in the gaps is easy to cause the mass propagation of the aerobic bacteria, so that the ensiling fails. Therefore, the proper storage time needs to be selected, the moisture content is adjusted, and the sugar loss in the early stage of ensiling is reduced.
Disclosure of Invention
The invention provides a method for adjusting the ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite, aiming at solving the technical problem of low quality of the existing sweet sorghum silage.
A method for regulating the ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite comprises the following steps:
firstly, removing leaves and roots of a whole plant of the mown sweet sorghum in the saline-alkali soil, and crushing to obtain filamentous crushed materials;
secondly, preparing a solution by adopting sodium metabisulfite or sodium metabisulfite;
the solution has a concentration of 800-2000 mg/L calculated according to sulfur dioxide;
thirdly, spraying the solution prepared in the second step on the filiform crushed aggregates obtained in the first step;
the spraying amount is 5-10% of the weight of the filiform crushed aggregates;
fourthly, filling the filiform crushed aggregates processed in the third step into a shading bag, exhausting air and sealing tightly to obtain a sweet sorghum silage bag;
and fifthly, storing the sweet sorghum silage bags obtained in the step four in a shady and cool place in a dark place for 8-16 days, and finishing the method.
Wherein, the solution prepared in the second step needs to be prepared as it is, and the metabisulfite and sulfur dioxide gas in the solution are not oxidized and lose effectiveness, and the service life of the solution is 6-12h, so that the solution is not suitable for being exposed in the air for a long time.
Wherein, the weight of the liquid sprayed in the second step is 5-10% of the mass of the material slag, because the optimal moisture content of the silage is 68-75%, and the sweet sorghum stalks belong to high-moisture raw materials, and excessive moisture is not easy to be added, the spraying amount is 5-10%.
And step four, putting the filiform crushed aggregates into a shading bag, paving the crushed aggregates layer by layer, compacting the crushed aggregates forcefully, discharging air in the bag, pressing the crushed aggregates again after filling the bag, and sealing the bag while pressing. This is because, in order to ensure a good lactic acid fermentation environment, it is necessary to form an anaerobic environment by exhausting the air in the sorgo slag to the maximum extent, and to enter lactic acid fermentation as soon as possible to suppress the growth of putrefying bacteria. And the bag is more convenient to store and transport, has better sealing performance and is not easy to damage the anaerobic environment.
And fifthly, storing the sweet sorghum silage bags in a shady and cool place in the dark, controlling the storage temperature to be 20-25 ℃, generating larger heat by microorganism activities, and storing the silage bags in the shady and cool place in the dark to prevent the temperature from rising and the silage from decaying due to direct sunlight. And the most suitable ensiling fermentation temperature is controlled to be 20-25 ℃.
And (3) storing the stored silage bags for about 8-16 days until the lowest pH value is reached, namely the microbial activity of the bags is weakened at the moment, and the lactic acid accumulation reaches the peak value, namely the sweet sorghum silage is mature. Put into practical application according to subsequent application scenes, such as being used as feed, substrates for continuous lactic acid fermentation and the like.
Pyrosulfites are different from other sulfites in that their aqueous solutions are acidic and have a strong sulfur dioxide odor, and can be used as an acidic bacteriostatic agent. Pyrosulfite is dissolved in water and then hydrolyzed into stable sodium bisulfite, which is a strong reducing agent and is easy to be oxidized in air to release sulfur dioxide gas. The sulfur dioxide-containing food preservative can consume oxygen in food, plays roles of deoxidation and oxidation resistance, can be used as an acidic bacteriostatic agent, sulfite can inhibit bacteria, fungi, mould and the like, and generated sulfur dioxide has a stronger bacteriostatic effect under an acidic condition.
In the case of ensiling, in the early stage of ensiling, the air remaining between the material residues can cause the movement of mold, yeast and putrefying bacteria except lactobacillus because the ensiling bag is not compacted. The addition of pyrosulfite can firstly reduce the pH value of the slag by utilizing the self-generated acidity of the solution, and consume the residual oxygen through deoxidation. The low pH and anoxic environment help slow the metabolic growth of harmful bacteria in silage, such as Clostridium butyricum, Acetobacter xylinum. When the silage goes by the storage time, the internal lactic acid bacteria metabolize to produce lactic acid, the pH value further gradually decreases, and a natural bacteriostatic environment is formed. The pyrosulfite is added to prevent enzymatic browning, and the trace nutrient components of the vitamins in the lining are effectively reserved. The pyrosulfite participates in the growth and metabolism of microorganisms through slow-release sulfur dioxide and sulfite radicals in the silage, and plays a role in regulating and controlling the flora of the silage.
The method can firstly utilize the self-generated acidity of the solution to reduce the pH value of the material slag by adding the pyrosulfite, and consumes the residual oxygen through the deoxidation effect. The low pH and anoxic environment help slow the metabolic growth of harmful bacteria in silage, such as Clostridium butyricum, Acetobacter xylinum. When the silage goes by the storage time, the internal lactic acid bacteria metabolize to produce lactic acid, the pH value further gradually decreases, and a natural bacteriostatic environment is formed. The pyrosulfite is added to prevent enzymatic browning, and the trace nutrient components of the vitamins in the lining are effectively reserved. The pyrosulfite participates in the growth and metabolism of microorganisms through slow-release sulfur dioxide and sulfite radicals in the silage, and plays a role in regulating and controlling the flora of the silage.
Through the application of the pyrosulfite additive, the pyrosulfite additive participates in the growth and metabolism process of microorganisms in the silage early, regulates the ecology of endophytic bacteria, and has great influence on the quality of the silage, such as color, smell and the like. This is important for higher sugar containing feedstocks such as sweet sorghum, reducing the production of unwanted metabolites such as ethanol, acetic acid, and the like. The bagged sweet sorghum is convenient to store and transport, the sweet sorghum planted in saline-alkali soil is often wide in planting area, and the storage and transport work after harvesting is very important. Through adding sodium metabisulfite salt solution, regulate and control sweet sorghum silage bacterial colony in earlier stage, reduce the sugar loss that leads to the fact in earlier stage because of other miscellaneous fungus metabolism, and then promote the relative abundance of lactic acid bacteria in the silage in later stage, can effectual accumulation lactic acid, keep sweet sorghum new freshness, can not corrupt for 2 months at room temperature promptly. The invention has the beneficial effects that:
according to the method, the saccharine sorghum stalks harvested in the saline-alkali soil are used for preparing the silage, the pyrosulfite solution with a certain concentration is added during preparation, so that the microbial flora in the material residues is adjusted in advance, the accumulation of acetic acid in the silage can be reduced, the improper consumption of sugar is avoided, the activity of lactic acid bacteria in the silage is adjusted, the saccharine sorghum silage is early, more lactic acid is accumulated, the breeding of other mixed bacteria or harmful bacteria is inhibited, and the quality of the saccharine sorghum silage is improved.
The invention prepares the sodium metabisulfite solution and then applies the sodium metabisulfite solution into the sweet sorghum slag in a spraying mode, has no secondary pollution, does not generate waste water, and has simple operation, safety and effectiveness.
The method is used for ensiling the sweet sorghum in the saline-alkali soil.
Drawings
FIG. 1 is a graph showing the dynamic effect of different concentrations of sodium metabisulfite on the pH of sweet sorghum silage;
FIG. 2 is a graph of lactic acid content measured using a biological multichannel sensing analyzer;
FIG. 3 is a yeast colony in a sweet sorghum silage bag;
FIG. 4 is a colony of Actinomycetes/spores in a sweet sorghum silage bag;
FIG. 5 shows a lactic acid bacteria colony from a sweet sorghum silage bag.
Detailed Description
The technical solution of the present invention is not limited to the specific embodiments listed below, and includes any combination of the specific embodiments.
The first embodiment is as follows: the embodiment provides a method for regulating the ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite, which specifically comprises the following steps:
firstly, removing leaves and roots of a whole plant of the mown sweet sorghum in the saline-alkali soil, and crushing to obtain filamentous crushed materials;
secondly, preparing a solution by adopting sodium metabisulfite or sodium metabisulfite;
the solution has a concentration of 800-2000 mg/L calculated according to sulfur dioxide;
thirdly, spraying the solution prepared in the second step on the filiform crushed aggregates obtained in the first step;
the spraying amount is 5-10% of the weight of the filiform crushed aggregates;
fourthly, filling the filiform crushed aggregates processed in the third step into a shading bag, exhausting air and sealing tightly to obtain a sweet sorghum silage bag;
and fifthly, storing the sweet sorghum silage bags obtained in the step four in a shady and cool place in a dark place for 8-16 days, and finishing the method.
The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: and step one, crushing by adopting a crusher. The rest is the same as the first embodiment.
The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the thickness of the filiform crushed aggregates obtained in the first step is 1-2 mm. The other is the same as in the first or second embodiment.
The fourth concrete implementation mode: the difference between this embodiment mode and one of the first to third embodiment modes is: the solvent for preparing the solution in the second step is water. The other is the same as one of the first to ninth embodiments.
The fifth concrete implementation mode is as follows: the difference between this embodiment and one of the first to fourth embodiments is: the solution prepared in the second step has the concentration of 1200-1600 mg/L calculated according to sulfur dioxide. The other is the same as one of the first to fourth embodiments.
The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is: and in the third step, the spraying amount of the solution is 6-8% of the mass of the filiform crushed aggregates. The other is the same as one of the first to fifth embodiments.
The seventh embodiment: the difference between this embodiment and one of the first to sixth embodiments is: and step three, uniformly spraying the solution while stirring. The other is the same as one of the first to sixth embodiments.
The specific implementation mode is eight: the present embodiment differs from one of the first to seventh embodiments in that: and step three, uniformly stirring by using a clean glass rod or two hands. The other is the same as one of the first to seventh embodiments.
The specific implementation method nine: the present embodiment differs from the first to eighth embodiments in that: the concrete operation of obtaining the sweet sorghum silage bags in the step four is as follows: spreading the filiform crushed aggregates layer by layer, compacting with force, discharging air, and pressing again after filling. The rest is the same as the first to eighth embodiments.
The detailed implementation mode is ten: the present embodiment differs from one of the first to ninth embodiments in that: and step five, the storage temperature is 20-25 ℃. The other is the same as one of the first to ninth embodiments.
The following examples were used to demonstrate the beneficial effects of the present invention:
the first embodiment is as follows:
the sweet sorghum used in the embodiment is planted in a certain saline-alkali soil in Daqing, and is harvested at the bottom of 9 months after the growth of 5-9 months. As more rainwater exists in 2020 and the topography of the planting field is lower, the growth vigor of the sweet sorghum is not good enough, the plant height is about 2 meters, and the yield is about 15kg/m 2 。
The compositional analysis of sweet sorghum is given in table 1 below:
TABLE 1
The method for regulating the ecology of the silage flora of the sweet sorghum in the saline-alkali soil by using the pyrosulfite comprises the following steps:
firstly, removing leaves and roots of a whole sweet sorghum plant in a mown saline-alkali soil, and crushing by using a crusher to obtain filamentous crushed materials, wherein the thickness of the filamentous crushed materials is 1-2 mm;
preparing a solution by adopting sodium metabisulfite, wherein the solution has the concentration of 400mg/L according to the calculation of sulfur dioxide;
thirdly, spraying the solution prepared in the second step on the filiform crushed aggregates obtained in the first step;
fourthly, filling 500g of the filiform crushed aggregates processed in the third step into a vacuum shading bag with an air extraction valve, pressing tightly, discharging air and sealing tightly to obtain the sweet sorghum silage bag;
and fifthly, placing the sweet sorghum silage bags obtained in the step four in a light-shielding constant-temperature incubator, keeping the storage temperature at 25 ℃ for 8-16 days, and finishing the method.
The solvent of the solution prepared in the second step is pure water which is in a third-level standard of the composite national standard GB 6682-2000;
and the spraying amount of the solution in the third step is 5 percent of the mass of the filiform crushed aggregates.
And step three, when the solution is sprayed, the solution is uniformly sprayed and stirred by adopting a clean glass rod or two hands.
The concrete operation of obtaining the sweet sorghum silage bags in the step four is as follows: spreading the filiform crushed aggregates layer by layer, compacting with force, discharging air, and pressing again after filling.
Example two:
the difference between the present embodiment and the first embodiment is: and step two, preparing a solution with the concentration of 800mg/L according to the calculated sulfur dioxide. The rest is the same as the first embodiment.
Example three:
the difference between the present embodiment and the first embodiment is: and step two, preparing a solution with the concentration of 1200mg/L according to the sulfur dioxide. The rest is the same as the first embodiment.
Example four:
the difference between the present embodiment and the first embodiment is: and step two, preparing a solution with the concentration of 1600mg/L according to sulfur dioxide. The rest is the same as the first embodiment.
Example five:
the difference between the present embodiment and the first embodiment is: and step two, preparing a solution with the concentration of 2000mg/L according to the calculated sulfur dioxide. The rest is the same as the first embodiment.
Control experiment: spraying the sweet sorghum filiform crushed aggregates by using pure water.
For the examples and control experiments, 10g of samples were taken per day for leaching and each index was tested.
FIG. 1 shows the concentration of sulfur dioxide versus sweet sorghumpH storage kinetics profiles, wherein "■" represents control experiments, "●" represents example one, ". tangle-solidup" represents example two, ". xxx" represents example three, ". diamond-solid" represents example four,
represents example five; as is apparent from fig. 1, the pH of the control group suddenly dropped below 3.7 within 2 days of ensiling, while the pH of the treatment group with sodium metabisulfite addition dropped to a level below 3.6 on day 8 with a more gradual drop in pH within 2 days. This indicates that the addition of sodium metabisulfite during ensiling slows the metabolic activity of the microorganisms during the early stage of ensiling. In addition, the control group was fluctuated at day 8 after the pH was decreased and then maintained at about 3.6, while the treatment group added with sodium metabisulfite was maintained at about 3.5 without significant fluctuation after the pH was decreased, indicating that the environment was stable in the latter stage of ensiling.
FIG. 2 is a graph of lactic acid content using a biological multichannel sensing analyzer, wherein "■" represents a control experiment, "●" represents example one, ". tangle-solidup" represents example two, ". xxx" represents example three, ". diamond-solid" represents example four,
represents example five; wherein the leaching liquor is filtered by a 0.45 mu m water-phase filter membrane, and the filtrate can be measured on a machine. As can be seen from fig. 2, unlike the pH change, the pH of the control group suddenly decreased at the early stage (2 days) of the ensiling, but the lactic acid content decreased somewhat, indicating that the pH decreased at the early stage, not lactic acid, but other acids were generated. Lactic acid begins to accumulate rapidly after 8 days of ensiling, which indicates that the lactic acid bacteria at the early stage are not dominant bacteria, other microorganisms generate more other acids such as acetic acid in the period, so that the pH value is greatly reduced, and a large amount of sugar of the sweet sorghum is consumed for metabolic activities of other miscellaneous bacteria in the first 8 days of ensiling. In each treatment group with sodium metabisulfite added, the period of lactic acid accumulation is consistent with the period of pH drop, which indicates that lactic acid bacteria are the dominant bacteria for producing acid, and the pH change amplitude is consistent with the lactic acid content change degree, which indicates that the sodium metabisulfite is added for inhibitingThe lactic acid bacteria have the advantages of ensuring the dominant position of the lactic acid bacteria, enabling the silage to start to accumulate lactic acid earlier and reducing other mixed acids, wherein the accumulation amount of the lactic acid in the sweet sorghum silage treated by 1200mg/L is the highest, and a small amount of lactic acid is accumulated in the early stage of the silage.
Preparing MRS liquid and solid culture medium, observing the distribution of microbial colonies in silage under different sodium metabisulfite concentrations, firstly, taking the eighth day leaching liquor with the highest silage lactic acid accumulation amount as a sample to be tested, sucking 1mL of sample liquid into 15mL of MRS liquid culture medium, and carrying out standing culture in a 37 ℃ incubator for 48 hours. And then culturing for 48 hours at 37 ℃ by a dilution coating plate method, observing the morphology of the bacterial colony, and judging whether the bacterial colony is lactobacillus according to whether a transparent ring is generated.
Three types of colony morphologies were observed on MRS medium: FIG. 3 shows that the colonies are beige, have regular edges, are sticky, protrude from a flat plate, and have no calcium-dissolving rings; FIG. 4 shows that the bacterial colony is rice white and whitish, has rough edge, convex center, flat periphery, no stickiness and no calcium dissolving ring; FIG. 5 shows that the colony is milky white, has a neat edge, is like water drops, stands on a flat plate, and is a transparent ring around the visible colony.
As can be seen from the analysis of colony morphology and state, FIG. 3 shows the yeast colony; FIG. 4 is a colony of actinomycetes or spores; FIG. 5 shows a lactic acid bacteria colony;
the plates were screened from the control group, in which the colony of FIG. 5 in the plate ensiled in the 1200mg/L treatment group was dominant bacteria, which was lactic acid bacteria as shown by the results of calcium-solubilizing circles, but in the other concentration treatment groups, the same dilution gradient (10) 4 -10 5 ) The colonies in FIG. 5 were not seen in the plates, the colonies in FIG. 3 were the dominant bacteria in the 400, 800g/L concentration treatment groups, the colonies in FIG. 5 appeared at lower dilution, and the colonies in FIG. 4 were slightly higher in number than the colonies in FIG. 5 in the 1600, 2000mg/L treatment groups. This shows that the difference in sodium metabisulfite concentration affects the composition of microbial colonies and lactic acid bacteria abundance in silage, resulting in differences in lactic acid content in silage for each treatment group. The 1200mg/L treatment group had the highest content of ensiled lactic acid and the corresponding lactic acid bacteria had the highest abundance. Also, FIG. 3 shows a larger drop in the number of colonies compared to the control group, with a dilution gradient (10) 4 -10 5 ) In the following, the first and second parts of the material,the colony number is reduced from 14 of the control group to 2 of the 400mg/L treatment group until 0 of the 1200-2000mg/L treatment group, which indicates that a certain concentration of sodium metabisulfite has a better inhibition effect on the yeast.
In summary, by applying a sodium metabisulfite solution with a certain concentration, the metabolic activity of microorganisms in silage can be influenced by the acidity of the sodium metabisulfite solution, the contained metabisulfite and sulfur dioxide slowly released along with time, and the specific expression is that the microbial metabolic activity of sweet sorghum dregs treated by the sodium metabisulfite solution is weakened in the early stage (2 days) of silage, less mixed acid is generated, and then the sweet sorghum dregs directly enter a lactic acid accumulation period, so that the sugar loss caused by the metabolism of residual air or mixed bacteria in a bag in the early stage is prevented. After more than 2 weeks of storage, the color of the sweet sorghum silage is not changed greatly, the sweet sorghum silage is yellow-green, the sweet sorghum silage has sour flavor, the silage is tightly held and cannot be scattered, no liquid is left, and the excellent quality of the sweet sorghum silage is ensured.
Claims (10)
1. A method for regulating the ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite is characterized by comprising the following steps:
firstly, removing leaves and roots of a whole plant of the mown sweet sorghum in the saline-alkali soil, and crushing to obtain filamentous crushed materials;
secondly, preparing a solution by adopting sodium metabisulfite or sodium metabisulfite;
the solution has a concentration of 800-2000 mg/L calculated according to sulfur dioxide;
thirdly, spraying the solution prepared in the second step on the filiform crushed aggregates obtained in the first step;
the spraying amount is 5-10% of the weight of the filiform crushed aggregates;
fourthly, filling the filiform crushed aggregates processed in the third step into a shading bag, exhausting air and sealing tightly to obtain a sweet sorghum silage bag;
and fifthly, storing the sweet sorghum silage bags obtained in the step four in a shady and cool place in a dark place for 8-16 days, and finishing the method.
2. The method for regulating the ecology of the silage flora of sweet sorghum in saline-alkali soil by using pyrosulfite as claimed in claim 1, wherein the step one is to crush by using a crusher.
3. The method for regulating the ecology of the silage flora of the sweet sorghum in the saline-alkali soil by using the pyrosulfite as claimed in claim 1, wherein the thickness of the filiform crushed aggregates obtained in the first step is 1-2 mm.
4. The method for regulating the ecology of the silage flora of sweet sorghum in saline-alkali soil by using pyrosulfite as claimed in claim 1, wherein the solvent of the solution prepared in the second step is water.
5. The method for regulating the ecology of the silage flora of the sweet sorghum in the saline-alkali soil by using the pyrosulfite according to claim 1, wherein the concentration of the solution prepared in the second step is 1200-1600 mg/L calculated according to sulfur dioxide.
6. The method for regulating the ecology of the silage flora of the sweet sorghum in the saline-alkali soil by using the pyrosulfite as claimed in claim 1, wherein the spraying amount of the solution in the third step is 6-8% of the mass of the filiform crushed aggregates.
7. The method for regulating the ecology of the silage flora of sweet sorghum in saline-alkali soil by using pyrosulfite as claimed in claim 1, characterized in that the solution is uniformly sprayed in the third step with stirring.
8. The method for regulating the ecology of the silage flora of the sweet sorghum in the saline-alkali soil by using the pyrosulfite as claimed in claim 7, wherein the third step is uniformly mixed by using a clean glass rod or two hands.
9. The method for regulating the ecology of the silage flora of sweet sorghum in saline-alkali soil by using pyrosulfite according to claim 1, wherein the concrete operation of obtaining the sweet sorghum silage bag in the fourth step is as follows: spreading the filiform crushed aggregates layer by layer, compacting with force, discharging air, and pressing again after filling.
10. The method for regulating the ecology of the silage flora of the sweet sorghum in the saline-alkali soil by using the pyrosulfite as claimed in claim 1, wherein the storage temperature in the fifth step is 20-25 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210575999.7A CN114886015A (en) | 2022-05-24 | 2022-05-24 | Method for regulating ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210575999.7A CN114886015A (en) | 2022-05-24 | 2022-05-24 | Method for regulating ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114886015A true CN114886015A (en) | 2022-08-12 |
Family
ID=82725156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210575999.7A Pending CN114886015A (en) | 2022-05-24 | 2022-05-24 | Method for regulating ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114886015A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115720965A (en) * | 2022-11-25 | 2023-03-03 | 沈阳农业大学 | Method for reducing nitrate nitrogen content of silage by using sulfite |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105851487A (en) * | 2016-04-28 | 2016-08-17 | 山东健源生物科技有限公司 | Additive for straw feed silaging and application |
| CN106798092A (en) * | 2015-11-26 | 2017-06-06 | 中创云牧(内蒙古)科技咨询有限公司 | The preparation method that a kind of sorghum wraps up in bag ensiling |
| CN108497175A (en) * | 2018-04-17 | 2018-09-07 | 贵州省草地技术试验推广站 | A kind of Silaging method removing fringe maize straw |
| CN108935972A (en) * | 2018-05-14 | 2018-12-07 | 中国科学院微生物研究所 | A kind of method of feeding soybean and the mixed storage of sugar grass |
| CN109156616A (en) * | 2018-09-14 | 2019-01-08 | 中国科学院寒区旱区环境与工程研究所 | Mixed silage feed and preparation method |
| CN112335779A (en) * | 2020-10-21 | 2021-02-09 | 宜宾学院 | Mixed silage of alpinia japonica and sweet sorghum and preparation method thereof |
-
2022
- 2022-05-24 CN CN202210575999.7A patent/CN114886015A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106798092A (en) * | 2015-11-26 | 2017-06-06 | 中创云牧(内蒙古)科技咨询有限公司 | The preparation method that a kind of sorghum wraps up in bag ensiling |
| CN105851487A (en) * | 2016-04-28 | 2016-08-17 | 山东健源生物科技有限公司 | Additive for straw feed silaging and application |
| CN108497175A (en) * | 2018-04-17 | 2018-09-07 | 贵州省草地技术试验推广站 | A kind of Silaging method removing fringe maize straw |
| CN108935972A (en) * | 2018-05-14 | 2018-12-07 | 中国科学院微生物研究所 | A kind of method of feeding soybean and the mixed storage of sugar grass |
| CN109156616A (en) * | 2018-09-14 | 2019-01-08 | 中国科学院寒区旱区环境与工程研究所 | Mixed silage feed and preparation method |
| CN112335779A (en) * | 2020-10-21 | 2021-02-09 | 宜宾学院 | Mixed silage of alpinia japonica and sweet sorghum and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115720965A (en) * | 2022-11-25 | 2023-03-03 | 沈阳农业大学 | Method for reducing nitrate nitrogen content of silage by using sulfite |
| CN115720965B (en) * | 2022-11-25 | 2024-05-14 | 沈阳农业大学 | Method for reducing nitrate nitrogen content of silage by utilizing sulfite |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103232944B (en) | Microorganism bacterium agent for straw and excrement mixed composting | |
| CN102617208B (en) | Edible fungus residue organic fertilizer raw materials and preparation method thereof | |
| CN109679860A (en) | A kind of composite bacteria agent and the preparation method and application thereof for the processing of gardens green waste | |
| CN102344326B (en) | Organic biofertilizer capable of inhibiting continuous cropping obstacle of flue-cured tobaccos | |
| CN102531801A (en) | Acidic microbial organic fertilizer and preparation method thereof | |
| CN101676385A (en) | Microbiological preparation capable of improving soil continuous cropping obstacle and production method thereof | |
| CN102173884B (en) | High-mountain vegetable waste microbiological treatment method | |
| US20180305656A1 (en) | Methods of preserving a microalgae biomass and a preserved microalgae biomass | |
| CN104744182A (en) | Tobacco water retention biological organic fertilizer and preparation method thereof | |
| CN105441360A (en) | Organic fertilizer fermentation inoculant | |
| CN110156511A (en) | A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material | |
| CN110591937B (en) | Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, method and application | |
| CN114702357B (en) | High-activity compound microbial fertilizer and preparation method thereof | |
| CN103724058A (en) | Microbial fermentation functional organic compound fertilizer and production method thereof | |
| CN114886015A (en) | Method for regulating ecology of sweet sorghum silage flora in saline-alkali soil by using pyrosulfite | |
| CN112919941A (en) | Method for preparing agricultural enzyme from perishable vegetable residues and inferior fruits and vegetables | |
| CN102146325B (en) | Method for culturing closed cellar mud | |
| CN104513752A (en) | Method for curing pit mud | |
| CN117467577B (en) | Sweet potato paste fermentation product and application thereof | |
| CN111559942A (en) | Organic liquid fertilizer and preparation method thereof | |
| CN101560482B (en) | Buprofezin pesticide residual degrading bacteria and produced microbial inoculum thereof | |
| CN107337488A (en) | A kind of preparation method of zinc-rich germ bran biological fertilizer | |
| CN111646844A (en) | Novel composite enzyme microbial fertilizer and preparation method thereof | |
| CN111685009A (en) | Planting method for improving per unit yield and sugar yield of sugarcane | |
| CN111893051A (en) | Microbial agent for sweet potato seedling and rapid propagation and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |