CN107337488A - A kind of preparation method of zinc-rich germ bran biological fertilizer - Google Patents
A kind of preparation method of zinc-rich germ bran biological fertilizer Download PDFInfo
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- CN107337488A CN107337488A CN201710470443.0A CN201710470443A CN107337488A CN 107337488 A CN107337488 A CN 107337488A CN 201710470443 A CN201710470443 A CN 201710470443A CN 107337488 A CN107337488 A CN 107337488A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention relates to a kind of preparation method of bio-fertilizer, specially a kind of preparation method of zinc-rich germ bran biological fertilizer, belong to biological technical field.Main technical schemes are as follows：Mushroom bran is dried, smash, sieved；The urea of mushroom bran weight 1% is taken, urea water is made by urea is soluble in water, urea water is mixed with mushroom bran, the water content after mixing in mushroom bran is 60%~65%；Naturally place 24 hours；Cotton-shaped rice bran is taken, it is well mixed with mushroom bran, obtains mixed bacteria bran；A zinc part present in mushroom bran is unabsorbed inorganic zinc sulfate, and the zinc remained in bacterial manure in substantial amounts of mycelium is present in the form of organic zinc, is more conducive to be absorbed and used by plants.The fermentation substrate of the present invention is edible fungus bran, and compared to traditional stalk or other matrix, mushroom bran more embodies the sustainable development of recycling for resource, more conducively resource.
Description
Technical field
The present invention relates to a kind of preparation method of bio-fertilizer, specially a kind of preparation method of zinc-rich germ bran biological fertilizer.
Background technology
Edible fungus bran refers to remaining discarded object, also referred to as bacteria residue after culturing edible fungus.Its main component is
It is made up of crops such as stalk, wood chip, cotton seed hulls, rice brans, these matrix by fermentation, all may be used by lignin and cellulose therein
With by different degrees of degraded, and it is converted into a variety of nutriments being utilized.Edible mushroom in the course of cultivation, with life
Length generates abundant mycelium, and small molecule nutriment is secreted out of under conditions of enzymolysis, wherein only a small amount of
Nutrition be used for the synthesis of itself mycoprotein, and remaining most nutriments are remained in culture matrix and mycelium, are made
Obtain in mushroom bran and be enriched abundant nutrition.Edible fungus bran can be used as feed, fertilizer or fuel etc. to use, but not have also at present
Have specifically for the method for preparing zinc-rich germ bran biological fertilizer.
The content of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of preparation method of zinc-rich germ bran biological fertilizer, the biology of preparation
Fertilizer is rich in zinc, and preparation method is simple and easy.Technical scheme is as follows：A kind of preparation side of zinc-rich germ bran biological fertilizer
Method, comprise the following steps：
(1) mushroom bran is dried, smashes, sieved after zinc-rich culturing edible fungus is harvested；
(2) urea of mushroom bran weight 1% is taken, urea water is made by urea is soluble in water, urea water is mixed with mushroom bran, is mixed
Water content after conjunction in mushroom bran is 60%~65%；
(3) mushroom bran prepared by step (2) is placed 24 hours naturally；
(4) cotton-shaped rice bran is taken, it is well mixed with mushroom bran, obtains mixed bacteria bran；The weight of the cotton-shaped rice bran is step
Suddenly the 5% of mushroom bran weight after (3) are handled；
(5) pH value of mixed bacteria bran is adjusted in the range of 6~9；
(6) mixed bacteria bran is placed in spontaneous fermentation in shading, ventilative closure container, when fermentation temperature reaches 58-62 DEG C
High temperature when, after continuous high temperature 24-36h, stir for the first time, stood after stirring；
(7) when temperature reaches 58-62 DEG C of high temperature again, stirred for the second time, 5-7 days are stood after stirring, until
Fermentation temperature is stable below 40 DEG C, and fermentation is completed, and zinc-rich germ bran biological fertilizer is made；
Microbial inoculum is mixed with mushroom bran described in step (6), the inoculum concentration of microbial inoculum is 1wt%, and described bacterial preparation process is such as
Under：Step (7) system is obtained into zinc-rich germ bran biological fertilizer with sterilized water according to 1g:9mL ratio mixing, is heated in 80 DEG C of water-baths
5min, gradient dilution is carried out, bacillus is isolated and purified out, the bacillus isolated and purified is preserved on slant medium,
And culture is enlarged by agitated submerged culture, microbial inoculum is made.
Further, Zn content is 397.58-437.58mg/kg in step (1) mushroom bran, and preferably Zn content is
417.58mg/kg.The existence form of zinc is zinc ion or organic zinc in described mushroom bran, organic zinc for example albumen-zinc, nucleic acid-zinc,
Polysaccharide-zinc.
Further, step (1) mushroom bran be sliding mushroom using bacterium bag as the bacterium bag residue after the completion of fermenting raw materials, it is described
Bacterium bag include the raw material of following percentage：Sawdust 60%, wood chip piece 30%, rice bran 3%, wheat bran 7%；PH is 5.8, water content
For 65%.The content of zinc is 800-1000mg/kg in described bacterium bag.
Beneficial effects of the present invention are as follows：(1) fermentation substrate of the invention is edible fungus bran, compared to traditional stalk
Or other matrix, mushroom bran more embody the sustainable development of recycling for resource, more conducively resource；(2) present in mushroom bran
A zinc part is unabsorbed inorganic zinc sulfate, and the zinc remained in bacterial manure in substantial amounts of mycelium is in the form of organic zinc
In the presence of being more conducive to be absorbed and used by plants.(3) separation and Extraction is beneficial to hair from the zinc-rich germ bran biological fertilizer after Natural compost fermentation
Microorganism-bacillus of ferment process, as microbial inoculum, and it is added in unfermentable mushroom bran, for strengthening ferment effect, more
Fermentation process is advantageously promoted soon so that fermentation is more thorough.(4) in composting process, whether moisture content directly affects heap in right amount
Fertile fermenting speed and decomposed degree, so moisture content is one of key factor of aerobic composting.If moisture content is too high, exceed
65%, it can cause that compost material is compacted or its internal free space is filled by moisture film, free voidage is reduced and is influenceed empty
The diffusion of gas, and make organic matter oxygen supply deficiency and anaerobic state occur, form smelly intermediate product (hydrogen sulfide, mercaptan, ammonia
Deng) produce stench and cause because of sulfide windrow corruption to black.Moisture in material is less than 40%, cannot meet microorganism
Growth need, the motion and metabolism of restriction micro-organisms, windrow centre is not reached suitable high temperature, organic matter is difficult to point
Solution, so as to reduce reaction rate.When moisture content be less than 30% when, microorganism absorbed nourishment in water material ability reduce, it is micro-
Biological self reproducing is slow, and organic matter decomposable process is made slow progress.(5) temperature is the reflection of compositing system microbial activities, is that influence is micro-
An important factor for biological activity and composting process process.The too low fermentation of compost temperature is not thorough；Temperature is too high, into sporogenesis
In the stage, make decomposition rate accordingly slack-off.Therefore, During High-Temperature Composting temperature is preferably at 58 DEG C -62 DEG C or so.Control the high temperature rank of fermentation
Section, it is possible to reduce the generation of the foul smell such as ammonia, sulfide.(6) added during zinc-rich culturing edible fungus in culture base-material inorganic
Zinc source, there is a part of unabsorbed inorganic zinc in the mushroom bran after culturing edible fungus, also remain in substantial amounts of mycelium
Zinc, this part zinc be in the form of the organic zinc such as protein zinc and polysaccharide zinc exist, be more conducive to be absorbed and used by plants.This hair
It is bright to obtain zinc-rich bio-fertilizer by fermentation substrate of zinc-rich edible fungus bran, zinc-rich crop is obtained by applying fertilizer, more embodies resource
Recycle, the more conducively sustainable development of resource.
Embodiment
With reference to specific embodiment, the invention will be further described.Following examples are descriptive, are not limits
Qualitatively, it is impossible to which protection scope of the present invention is limited with this.The chemical reagent and instrument that the present invention is applied are such as without special
Illustrate, can be bought from commercial channel.
Embodiment 1：The preparation of mushroom bran
(1) matrix：Sawdust 60%, wood chip piece 30%, rice bran 3%, wheat bran 7%；PH is 5.8, water content 65%, to bacterium
Zinc sulphate powder is admixed in bag, is made in every kilogram of bacterium bag containing the zinc-rich bacterium bag that zinc sulfate is 1000mg.
(2) sterilize：100 DEG C, 90 minutes -110 DEG C, 90 minutes -121 DEG C, 150 minutes-stewing 30 minutes-steam discharge of pot.
(3) it is inoculated with：Force to be cooled to below 18 DEG C of material temperature using chilling room and be inoculated with, inoculation is using artificial infection, manually envelope
Mouth machine sealing.
(4) cultivate：Bacterium germination room is sent into after inoculation to cultivate.
(5) acquisition of mushroom bran：After sliding mushroom growth cycle and thoroughly terminating (about 10 months), it can remain in matrix and not be eaten
The zinc that bacterium thoroughly absorbs, it is mushroom bran.
Embodiment 2：The preparation for mushroom bran of fermenting
(1) mushroom bran is dried, smashed, sieved；
(2) urea of mushroom bran weight 1% is taken, urea water is made by urea is soluble in water, urea water is mixed with mushroom bran, is mixed
Water content after conjunction in bacterial manure is 65%；
(3) mushroom bran prepared by step (2) is placed 24 hours naturally；
(4) cotton-shaped rice bran is taken, it is well mixed with mushroom bran, obtains mixed bacteria bran；The weight of the cotton-shaped rice bran is step
Suddenly the 5% of mushroom bran weight after (3) are handled；
(5) pH value for mixing bacterial manure is adjusted to 9；
(6) mixed bacteria bran is placed in spontaneous fermentation in shading, ventilative closure container, when fermentation temperature reaches 62 DEG C of height
After Wen Shi, continuous high temperature 36h, stir for the first time, stood after stirring；
(7) when temperature reaches 62 DEG C of high temperature again, stirred for the second time, 7 days are stood after stirring, until fermentation temperature
Degree is stable below 40 DEG C, and fermentation is completed, and fermentation mushroom bran is made.
Embodiment 3：The preparation of microbial inoculum
(1) configuration of broth bouillon：Beef extract 3g, peptone 10g, agar 20g, running water 1000mL.Adjust pH to
7.2, sterilized at a temperature of 121 DEG C of high-pressure sterilizing pot.Go out to be poured into culture dish after bacterium and flat board is made.
(2) strain isolates and purifies
The fermentation mushroom bran 10g of the preparation of embodiment 2 is weighed in 90mL sterilized waters, 5min is heated in 80 DEG C of water-baths, this ladder
Spend for 10-1, 10 are diluted to by its 10 times-6, take 10-4、10-5、10-6Each 0.1ml is coated on the culture medium prepared, each gradient
Apply 3 flat boards.After coating 48h is cultivated in 37 DEG C of constant incubators.Take 10-5The microorganism of gradient carries out microscopy, is chosen
It is drawn in culture on inclined-plane to save backup, is found to isolate polytype bacillus from experiment with sediments microscope inspection, with this
As compound gemma bacillus agent.
(3) preparation of microbial inoculum
The thalline isolated is cultivated on slant medium, after thalline covers with inclined-plane, picking thalline is inoculated in
In 250mL triangular flasks equipped with 100mL meat soup fluid nutrient mediums, 37 DEG C, 150r/min shaken cultivation 12h, then connect by 1%
In kind amount access mushroom bran.
Embodiment 4：The preparation of zinc-rich germ bran biological fertilizer
(1) microbial inoculum made of embodiment 3 is added in the mixed bacteria bran described in the step of embodiment 2 (6), according to embodiment
2 the step of, is fermented, and product is zinc-rich germ bran biological fertilizer.
The zinc-rich germ bran biological fertilizer prepared according to the method described above：Zn content is 415.4mg/kg；Nitrogen, phosphorus, potassium content summation
For 8.1%, meet the standard (>=5.0%) of total nutrient in Ministry of Agriculture's organic fertilizer standard；The content of organic matter is respectively 48.4%, symbol
Close the standard (>=45%) in national standard；P is 7.5, meets the scope (5.5-8.5) of national standard acid-base value.
Embodiment 5：Utilize zinc-rich germ bran biological fertilizer plantation zinc-rich pakchoi
When zinc-rich slide mushroom germ bran organic fertilizer be manured into soil middle ratio for 25% when, the germination percentage of four seasons pakchoi is 100%,
Yield is 2.95kg/m2, Vitamin C content 23.5mg/100g, soluble sugar content 2.10%, content of reducing sugar be
5.35%th, Zn content 9.85mg/kg.Compared with non-zinc-rich bacterial manure group, the Vitamin C content increase by 13.34% in pakchoi
(P<0.05)；Soluble sugar content increases by 0.79% (P<0.05)；Content of reducing sugar increases by 1.26% (P<0.05)；Nitrate contains
Amount reduces by 25.13% (P<0.01).Therefore, it is bright to illustrate that the zinc in zinc-rich germ bran organic fertilizer has to four seasons Growth of Cabbage and nutrition
Aobvious facilitation.
The specific embodiment of the invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment,
In those skilled in the art's possessed knowledge, various changes can also be made without departing from the inventive concept of the premise
Change.
Claims (4)
1. a kind of preparation method of zinc-rich germ bran biological fertilizer, it is characterised in that comprise the following steps：
(1) mushroom bran is dried, smashes, sieved after zinc-rich culturing edible fungus is harvested；
(2) urea of mushroom bran weight 1% is taken, urea water is made by urea is soluble in water, urea water is mixed with mushroom bran, after mixing
Water content in mushroom bran is 60%~65%；
(3) mushroom bran prepared by step (2) is placed 24 hours naturally；
(4) cotton-shaped rice bran is taken, it is well mixed with mushroom bran, obtains mixed bacteria bran；The weight of the cotton-shaped rice bran is step (3)
The 5% of mushroom bran weight after processing；
(5) pH value of mixed bacteria bran is adjusted in the range of 6~9；
(6) mixed bacteria bran is placed in spontaneous fermentation in shading, ventilative closure container, when fermentation temperature reaches 58-62 DEG C of height
After Wen Shi, continuous high temperature 24-36h, stir for the first time, stood after stirring；
(7) when temperature reaches 58-62 DEG C of high temperature again, stirred for the second time, 5-7 days are stood after stirring, until fermentation
Temperature stabilization below 40 DEG C, complete by fermentation, and zinc-rich germ bran biological fertilizer is made；
Microbial inoculum is mixed with mushroom bran described in step (6), the inoculum concentration of microbial inoculum is 1wt%, and described bacterial preparation process is as follows：Will
Step (7) system obtains zinc-rich germ bran biological fertilizer with sterilized water according to 1g:9mL ratio mixing, 5min is heated in 80 DEG C of water-baths,
Gradient dilution is carried out, bacillus is isolated and purified out, the bacillus isolated and purified is preserved on slant medium, and passes through
Agitated submerged culture is enlarged culture, and microbial inoculum is made.
2. the preparation method of zinc-rich germ bran biological fertilizer as claimed in claim 1, it is characterised in that in step (1) mushroom bran
Zn content is 397.58-437.58mg/kg.
3. the preparation method of zinc-rich germ bran biological fertilizer as claimed in claim 1, it is characterised in that Zn content in described mushroom bran
For 417.58mg/kg.
4. the preparation method of zinc-rich germ bran biological as claimed in claim 1 fertilizer, it is characterised in that step (1) mushroom bran is
Sliding mushroom includes the raw material of following percentage by the bacterium bag residue after the completion of fermenting raw materials, described bacterium bag of bacterium bag：Sawdust
60%th, wood chip piece 30%, rice bran 3%, wheat bran 7%；PH is 5.8, water content 65%, and the content of zinc is 800-1000mg/kg.
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| CN201711358023.XA CN108017430A (en) | 2017-06-20 | 2017-06-20 | A kind of preparation method of germ bran biological fertilizer |
| CN201710470443.0A CN107337488A (en) | 2017-06-20 | 2017-06-20 | A kind of preparation method of zinc-rich germ bran biological fertilizer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901600A (en) * | 2018-08-09 | 2018-11-30 | 合肥福泉现代农业科技有限公司 | A kind of method that expired milk recycles cultivation milk fragrance edible mushroom |
| CN108997047A (en) * | 2018-08-31 | 2018-12-14 | 大连民族大学 | A kind of selenium-rich germ bran biological fertilizer preparation method improving vegetables activities of antioxidant enzymes and nutritional quality |
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| CN1807362A (en) * | 2006-01-06 | 2006-07-26 | 大连广垠生物农药有限公司 | Preparation method of fermented bacterium slurry of lignocellulose organic fertilizer |
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2017
- 2017-06-20 CN CN201711358023.XA patent/CN108017430A/en active Pending
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| CN1807362A (en) * | 2006-01-06 | 2006-07-26 | 大连广垠生物农药有限公司 | Preparation method of fermented bacterium slurry of lignocellulose organic fertilizer |
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Cited By (2)
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| CN108901600A (en) * | 2018-08-09 | 2018-11-30 | 合肥福泉现代农业科技有限公司 | A kind of method that expired milk recycles cultivation milk fragrance edible mushroom |
| CN108997047A (en) * | 2018-08-31 | 2018-12-14 | 大连民族大学 | A kind of selenium-rich germ bran biological fertilizer preparation method improving vegetables activities of antioxidant enzymes and nutritional quality |
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Application publication date: 20171110 |