CN114832029A

CN114832029A - Mongolian medicine compound for preventing and treating calf diarrhea and preparation method and application thereof

Info

Publication number: CN114832029A
Application number: CN202210604282.0A
Authority: CN
Inventors: 郭志廷; 李建喜; 张康; 张景艳; 张凯; 王磊; 仇正英; 王玲; 徐国伟
Original assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Current assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority date: 2022-05-31
Filing date: 2022-05-31
Publication date: 2022-08-02
Anticipated expiration: 2042-05-31
Also published as: CN114832029B

Abstract

The invention belongs to the technical field of Mongolian medicines, and particularly relates to a Mongolian medicine compound for preventing and treating calf diarrhea, a preparation method and application thereof, wherein the Mongolian medicine compound comprises the following components in parts by weight: 30-60 parts of Mongolian kalimeris herb, 15-30 parts of rhizoma atractylodis macrocephalae, 25-50 parts of sophora alopecuroides, 10-20 parts of radix glehniae, 10-20 parts of starwort root and 5-10 parts of liquorice; the preparation method comprises weighing the above materials respectively, pulverizing, mixing, decocting in water, filtering to remove residue, sterilizing at high temperature, and packaging. The mongolian medicine compound for preventing and treating calf diarrhea provided by the invention is scientific and reasonable in design, has the advantages of definite curative effect, quick action, safety in use, no toxic or side effect and zero residue, and can greatly reduce the use amount of antibiotics in the breeding of cows and beef cattle.

Description

Mongolian medicine compound for preventing and treating calf diarrhea and preparation method and application thereof

Technical Field

The invention belongs to the technical field of Mongolian medicines, and particularly relates to a Mongolian medicine compound for preventing and treating calf diarrhea, and a preparation method and application thereof.

Background

The inner Mongolia autonomous region is located in northern Jianjiang and spans northeast, northeast and northwest of China, the terrain is complex, the climate is various, and the distribution of germplasm resources of medicinal plants is wide. The Chinese traditional medicine system divides China into 9 primary traditional Chinese medicine areas and 28 secondary areas. The inner Mongolia occupies 3 primary traditional Chinese medicine areas and 6 secondary traditional Chinese medicine areas in areas I, VI and VII, and has unique medicinal plant germplasm resources. According to records of 'inner Mongolia plant record', inner Mongolia has 2176 wild plants belonging to 131 families and 720 genera, including 1198 medicinal plants, and more than 200 varieties can be developed on a large scale.

The Mongolian medicine makes important contribution to the disease prevention and treatment of people of various families in Mongolian regions by unique and complete medicine theory, disease diagnosis and treatment technology system and abundant medicinal resources. However, because of the limitation of language and characters, and the ancient prescription from which most of Mongolian medicines come, compared with the traditional Chinese medicine, the Mongolian medicines have fewer new varieties and are not well popularized in the market.

Calf diarrhea, also known as calf diarrhea, can occur throughout the year and is a gastrointestinal disease frequently occurring in calves. The calf usually starts to attack disease 2-3 days after birth, and the growth, development, survival and the like of the calf are greatly influenced. When the calf diarrhea is raised in a large group, the incidence rate of the calf diarrhea is usually 90-100%, the mortality rate is more than 50%, and the development threat to the dairy industry in China is great. For a long time, the calf diarrhea treatment in a pasture is basically implemented by intramuscular injection of antibiotics, but on one hand, the use of a large amount of antibiotics easily causes the drug resistance of pathogenic bacteria to reduce the treatment effect of the drugs, and on the other hand, the balance of normal intestinal flora of calves is destroyed, and the growth and development of the calves are influenced.

Disclosure of Invention

The invention aims to overcome the defects of the existing antibiotics and chemical synthesis medicines for preventing and treating calf diarrhea, provides a Mongolian medicine compound for preventing and treating calf diarrhea and a preparation method thereof, and is applied to preventing and treating calf diarrhea.

In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:

the invention provides a Mongolian medicine compound for preventing and treating calf diarrhea, which comprises the following components in parts by weight: 30-60 parts of Mongolian kalimeris herb, 15-30 parts of rhizoma atractylodis macrocephalae, 25-50 parts of sophora alopecuroides, 10-20 parts of radix glehniae, 10-20 parts of starwort root and 5-10 parts of liquorice.

Further, in the Mongolian medicine compound, the Mongolian medicine compound comprises the following components in parts by weight: 40-50 parts of Mongolian kalimeris herb, 20-25 parts of rhizoma atractylodis macrocephalae, 30-45 parts of sophora alopecuroides, 12-18 parts of radix glehniae, 12-18 parts of starwort root and 6-9 parts of liquorice.

Further, in the Mongolian medicine compound, the Mongolian medicine compound comprises the following components in parts by weight: 45 parts of Mongolian kalimeris herb, 22.5 parts of rhizoma atractylodis macrocephalae, 37.5 parts of sophora alopecuroides, 15 parts of radix glehniae, 15 parts of starwort root and 7.5 parts of liquorice.

The invention provides a preparation method of a Mongolian medicine compound, which comprises the following steps:

1) weighing Mongolian kalimeris herb, rhizoma atractylodis, sophora alopecuroides, radix glehniae, starwort root and liquorice according to the proportion, and weighing for later use;

2) pulverizing Mongolian herba Kalimeridis, rhizoma Atractylodis, herba Sophorae Alopecuroidis, radix Glehniae, radix Stellariae and Glycyrrhrizae radix, and mixing to obtain mixture;

3) decocting the obtained mixture with water, and concentrating the decoction until each milliliter of oral liquid contains 0.75-1.25 g of crude drug;

4) filtering the residue, sterilizing at high temperature, and packaging.

Further, in the preparation method, in the step 3), the ratio of the total mass of the mixture to the volume of the added water is 1: 6-10 in terms of mass-to-volume ratio.

Further, in the preparation method, in the step 3), the mixture is decocted for 2-3 times, each time lasts for 1-2 hours, and the decoction liquid of each time is combined for concentration.

Further, in the preparation method, in the step 3), the mixture is soaked in advance for 1-2 hours before the first decoction.

The invention provides an application of a Mongolian medicine compound for preventing and treating calf diarrhea, which comprises the following steps: after the Mongolian medicine compound is prepared, the calf is drenched, and the calf diarrhea is treated and prevented.

Furthermore, when the Mongolian medicine compound is used for treating calf diarrhea, 50-100 mL of liquid Mongolian medicine compound is drenched for each head for 3-5 days. When calf diarrhea is prevented, 25-50 mL of liquid Mongolian medicine compound is drenched into each head for 3-5 days.

The invention has the beneficial effects that:

1. mongolian grassland is long in winter, cold in climate and thin in air, and local animals and plants have strong vitality, so that the traditional Chinese medicine (also belonging to plants) has high content of effective components and unique effective components (the same traditional Chinese medicine, and the effective components of inner Mongolian production areas and southern production areas are different). The medicines in the mongolian medicine compound for preventing and treating calf diarrhea provided by the invention are all mongolian medicines, have the advantages of definite curative effect, quick action, safety in use, no toxic or side effect and zero residue, and can greatly reduce the use amount of antibiotics in the breeding of cows and beef cattle.

2. According to the Mongolian medicine compound, Mongolian Indian kalimeris herb, rhizoma atractylodis and sophora alopecuroides clear away heat and toxic materials, dry dampness and invigorate spleen, coastal glehnia root and starwort root nourish yin to clear away the lung-heat, benefit stomach and promote fluid production, and licorice tonify spleen and replenish qi and moderate medicine property, the formula is carried out according to Mongolian medicine pharmacology theory, the Mongolian medicine compound has good effect of preventing and treating calf diarrhea through clinical verification, and is particularly suitable for the condition that the calf is easily infected with pathogenic microorganisms due to weakness of spleen and stomach and poor autoimmunity.

3. According to the invention, the formula of Mongolian kalimeris herb, rhizoma atractylodis, sophora alopecuroides, radix glehniae, starwort root and liquorice is determined through wide Mongolian medicine screening and clinical curative effect verification in the early stage; compared with the traditional Chinese medicine on the market, the traditional Chinese medicine has the advantages of easily available raw materials, simple preparation process, convenience for industrial production and wide clinical application prospect.

Of course, it is not necessary for any one product that embodies the invention to achieve all of the above advantages simultaneously.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram showing the number of Escherichia coli in calf feces before and after administration in example 8 of the present invention;

wherein (a) refers to diarrhea and (b) refers to healing.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The preparation method of the Mongolian medicine compound comprises the following steps:

3) and adding water into the obtained mixture for decoction, wherein the ratio of the total mass of the mixture to the volume of the added water is 1: 6-10 in terms of mass-volume ratio. The decocting times of the mixture are 2-3 times, each time lasts for 1-2 hours, and decoction liquid for each time is combined and concentrated. The mixture is soaked for 1-2 h before the first decoction. Concentrating the decoction until each milliliter of oral liquid contains 0.75 to 1.25 grams of crude drug;

4) filtering the residue, sterilizing at high temperature, and packaging.

The specific embodiment of the invention is as follows:

example 1

A Mongolian medicine compound for preventing and treating calf diarrhea is prepared by weighing the following medicines, 45 parts of Mongolian kalimeris herb, 20 parts of rhizoma atractylodis macrocephalae, 30 parts of sophora alopecuroides, 15 parts of radix glehniae, 10 parts of starwort root and 8 parts of liquorice. Pulverizing, adding water at a volume-to-mass ratio of 8:1, decocting for 3 times, each for 2h (soaking for 1h before the first decoction), mixing decoctions, concentrating until each ml of oral liquid contains 1.15 g of crude drug, filtering, sterilizing at high temperature, and packaging. Named Mongolian medicine No. 1.

Example 2

A Mongolian compound medicine for preventing and treating calf diarrhea is prepared from 40 parts of Mongolian milkwort herb, 25 parts of rhizoma atractylodis macrocephalae, 30 parts of sophora alopecuroide, 15 parts of radix glehniae, 15 parts of starwort root and 6 parts of liquorice root by weight. Pulverizing, adding water at a volume-to-mass ratio of 6:1, decocting for 2 times (2.0 hr each time (soaking for 1.5 hr before first decoction), mixing decoctions, concentrating to obtain oral liquid containing crude drug 1.05 g/ml, filtering, sterilizing at high temperature, and packaging.

Example 3

A Mongolian medicine compound for preventing and treating calf diarrhea is prepared from 45 parts of Mongolian milkwort herb, 22.5 parts of rhizoma atractylodis macrocephalae, 37.5 parts of sophora alopecuroides, 15 parts of radix glehniae, 15 parts of starwort root and 7.5 parts of liquorice by weight. Pulverizing, adding water at a volume-to-mass ratio of 9:1, decocting for 2 times (soaking for 1h before the first decoction), mixing decoctions, concentrating to obtain oral liquid containing crude drug 1.20 g per ml, filtering, sterilizing at high temperature, and packaging.

Example 4

A Mongolian medicine compound for preventing and treating calf diarrhea is prepared from 35 parts of Mongolian milkwort herb, 25 parts of rhizoma atractylodis macrocephalae, 45 parts of sophora alopecuroides, 20 parts of radix glehniae, 15 parts of starwort root and 5 parts of liquorice by weight. Pulverizing, adding water at a volume-to-mass ratio of 10:1, decocting for 3 times (1 h each time (soaking for 1.5h before the first decoction), mixing decoctions, concentrating to obtain oral liquid containing crude drug 0.96 g/ml, filtering, sterilizing at high temperature, and packaging.

Example 5

A Mongolian medicine compound for preventing and treating calf diarrhea is prepared from 60 parts of Mongolian kalimeris herb, 20 parts of rhizoma atractylodis macrocephalae, 30 parts of sophora alopecuroides, 15 parts of radix glehniae, 10 parts of starwort root and 6 parts of liquorice by weight. Pulverizing, adding water at a volume-to-mass ratio of 8:1, decocting for 2 times (soaking for 1h before the first decoction), mixing decoctions, concentrating to obtain oral liquid containing crude drug 1.19 g per ml, filtering, sterilizing at high temperature, and packaging.

Example 6

A Mongolian medicine compound for preventing and treating calf diarrhea is prepared from 55 parts of Mongolian milkwort herb, 20 parts of rhizoma atractylodis macrocephalae, 35 parts of sophora alopecuroides, 10 parts of radix glehniae, 15 parts of starwort root and 10 parts of liquorice by weight. Pulverizing, adding water at a volume-to-mass ratio of 7:1, decocting for 3 times (1 h each time (soaking for 1.5h before the first decoction), mixing decoctions, concentrating until each ml of oral liquid contains 1.08 g of crude drug, filtering, sterilizing at high temperature, and packaging.

Example 7

Toxicology test evaluation of Mongolian medicine No. 1

The experimental materials and methods were as follows:

1) test animals: 60 Kunming clean-grade mice are purchased from the experimental animal center of Lanzhou university (Ganzhijia moving character 14-005), have the weight of 18.0-22.0 g, are infertile and are raised in a squirrel cage at 21-24 ℃ for free feeding and drinking.

2) The tested drugs are: mongolian medicine No. 1 prepared in example 1.

3) Pre-testing: feeding observation is carried out on the mice for 7 days before the test, the mice freely feed and drink water in the observation period, the mice are weighed on empty stomach every day, and the mice which die naturally are eliminated. The mice were randomly divided into 4 groups of 6 mice each, half of the mice were male and female, and the mice were gavaged at doses of 3.00, 6.00, 9.00, 15.00g/kg body weight to determine the dose range for the official test.

4) And (3) formal test: healthy mice were randomly divided into 6 groups (blank control group and 5 dosing group), 10 mice per group, and males and females.

In the administration group, the administration is performed by intragastric administration according to the dose of 3.00, 4.20, 5.88, 8.23 and 11.52g/kg body weight respectively, and the dose ratio among the groups is 1: 1.4. Fasting (free drinking water) is carried out for 8 hours before and 4 hours after the gavage, the dosage is calculated according to the weight of each mouse, and the mice are orally administrated by a metal stomach tube for one-time gavage. In the placebo group, mice were not dosed, and were fed ad libitum and had water.

After administration, mice were observed daily for 7 days for ingestion, drinking, mental, death, hair loss, autonomic activity, and the like. Dead mice were dissected in time, the pathological changes were recorded, and all surviving mice were weighed and dissected on day 8 to observe pathological changes in the organs.

And (3) analyzing an experimental result:

the result of a preliminary test shows that the lethal dose range of the Mongolian medicine No. 1 to the mouse is 3.00-15.00 g/kg of body weight. The 95% confidence limit of LD50 and LD50 is calculated by modified Kouyange method in formal test, and the result shows that the Mongolian medicine No. 1 has LD50 of 7.75g/kg body weight and the 95% confidence limit of LD50 of 6.31-9.06 g/kg body weight. See tables 1 and 2:

TABLE 1 acute toxicity Pre-test results for Mongolian medicine No. 1

Group of	Dosage (g/kg)	Animal number (only)	Number/total of deaths
				1	3.00	6	0/6
2	6.00	6	2/6
				3	9.00	6	4/6
4	15.00	6	6/6

TABLE 2 acute toxicity official test results for Mongolian medicine No. 1

The acute toxicity test is an important index for evaluating the safety of a foreign compound, the oral LD50 of Mongolian medicine No. 1 to a mouse obtained by calculation by adopting an improved Kouyan method is 7.75g/kg of body weight, according to the acute toxicity test regulation in the veterinary medicine test technical specification compilation, in the acute toxicity classification standard of a foreign chemical, the mouse once oral LD50 is in the range of 0.5-5 g/kg, and the test is judged to be in a low-toxicity or actual non-toxicity category, so that the safety and reliability of the clinical medication of the Mongolian medicine No. 1 are prompted.

Example 8

Experimental study on Mongolian medicine No. 1 for preventing and treating calf diarrhea

The experimental materials and methods were as follows:

1) main test materials: mongolian medicine No. 1 was prepared according to example 1. The Escherichia coli chromogenic medium was purchased from Kyork, Guangdong, Microscience and technology Ltd. Colony Doc-It Colony counter is available from Beijing Saiboaiko technology, Inc.

2) The source of the cases: from 5 months to 10 months in 2021, a cattle farm was selected from Honggu district of Lanzhou city, inner Mongolia autonomous region Tongliao city, Nemanthu, Ke left and right flags, and Hunhait left flag, respectively, calves with diarrhea occurring naturally were collected, and preliminary diagnosis was made by epidemiological investigation and clinical symptom observation, and then confirmed by laboratory bacteriological examination.

3) And (3) drug treatment: the medicine is administered for 2 times per day for 3-5 days, wherein the dosage is 50-100 mL per day for treating each sick cow. The medicine is administered for 2 times per day for 2-3 days, and 25-50 mL of the medicine is taken every day by each sick cow. The medicine is shaken well before use and taken after being infused with warm water.

4) The clinical prevention and treatment effect is as follows: after administration, the mental state, diet, drinking water, defecation and the like of the sick cattle are observed every day. The diarrhea symptoms of the cattle with the drug are completely disappeared, the food intake and the water intake are recovered to be normal, the mental state is good, and the cattle with the drug is judged to be cured.

5) And (3) checking escherichia coli: on the basis of a common blood plate culture medium test, a feces sample of clinically collected diarrhea calves is diluted by 200 times, then is directly inoculated to an escherichia coli chromogenic culture medium by adopting a coating method, is marked, is placed in an incubator at 35-39 ℃ for 24 hours in a dark place for culture, and then the result is observed.

And (3) analyzing an experimental result:

1) control effect on calf diarrhea

The results show that the Mongolian medicine No. 1 has good treatment and prevention effects on calf diarrhea, the cure rate of the diarrhea of calves in Nemantu and Ke of inner Mongolia Tongliao city, Tu Yu of Huanhaite city and Red valley region of Gansu Lanzhou city reaches more than 95.0 percent, and the prevention rate can reach 100.0 percent at most. See tables 3 and 4:

TABLE 3 therapeutic effect of Mongolian medicine No. 1 on calf diarrhea

TABLE 4 preventive effect of Mongolian medicine No. 1 on calf diarrhea

2) Results of Escherichia coli detection

As shown in FIG. 1, after 24 hours of cultivation in the E.coli chromogenic medium, E.coli showed a blue-green color. The number of Escherichia coli detected by feces of diarrhea calves is large, and the number of Escherichia coli detected by feces of calves cured by the administration of Mongolian medicine No. 1 is remarkably reduced.

Mongolian medicine No. 1 has good prevention and treatment effects on calf diarrhea, the cure rate is over 95.0%, and the prevention rate can reach 100% at most. Bacteriological test results also show that the number of pathogenic escherichia coli in calf feces is remarkably reduced or even disappears after the calf feces are cured by administration. The test results suggest that Mongolian medicine No. 1 has the characteristics of safety, high efficiency, greenness and environmental protection, and is expected to be developed into a new medicine for preventing and treating calf diarrhea.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A Mongolian medicine compound for preventing and treating calf diarrhea is characterized by comprising the following components in parts by weight: 30-60 parts of Mongolian kalimeris herb, 15-30 parts of rhizoma atractylodis macrocephalae, 25-50 parts of sophora alopecuroides, 10-20 parts of radix glehniae, 10-20 parts of starwort root and 5-10 parts of liquorice.

2. The Mongolian medicine compound as claimed in claim 1, which comprises the following components in parts by weight: 40-50 parts of Mongolian kalimeris herb, 20-25 parts of rhizoma atractylodis macrocephalae, 30-45 parts of sophora alopecuroides, 12-18 parts of radix glehniae, 12-18 parts of starwort root and 6-9 parts of liquorice.

3. The Mongolian medicine compound as claimed in claim 2, which comprises the following components in parts by weight: 45 parts of Mongolian kalimeris herb, 22.5 parts of rhizoma atractylodis macrocephalae, 37.5 parts of sophora alopecuroides, 15 parts of radix glehniae, 15 parts of starwort root and 7.5 parts of liquorice.

4. A process for the preparation of a Mongolian medicinal composition according to any one of claims 1 to 3, which comprises the steps of:

4) filtering the residue, sterilizing at high temperature, and packaging.

5. The method of claim 4, wherein: in the step 3), the ratio of the total mass of the mixture to the volume of the added water is 1: 6-10 in terms of mass-volume ratio.

6. The method of claim 4, wherein: in the step 3), the decocting times of the mixture are 2-3 times, each time lasts for 1-2 hours, and the decoction liquid of each time is combined for concentration.

7. The method of claim 6, wherein: in the step 3), the mixture is soaked in advance for 1-2 hours before the first decoction.

8. Use of the Mongolian medicinal composition according to any one of claims 1-3 for treating and preventing calf diarrhea.

9. Use according to claim 8, characterized in that: when calf diarrhea is treated, 50-100 mL of liquid Mongolian medicine compound is drenched into each head for 3-5 days.

10. Use according to claim 8, characterized in that: when calf diarrhea is prevented, 25-50 mL of liquid Mongolian medicine compound is drenched into each head of calf, and the liquid Mongolian medicine compound is continuously used for 3-5 days.