CN114705875A

CN114705875A - Full-automatic light-activated chemiluminescence detector

Info

Publication number: CN114705875A
Application number: CN202210283767.4A
Authority: CN
Inventors: 吴栋杨; 刘贵东; 刘宇卉; 李临
Original assignee: Chemclin Diagnostics Corp
Current assignee: Chemclin Diagnostics Corp
Priority date: 2017-03-28
Filing date: 2018-02-06
Publication date: 2022-07-05
Also published as: CN108663529B; CN106980024A; CN108663530A; CN108663529A; CN108663530B; CN114705876A; CN115728499A; CN114839389A; CN115015565A; CN114675043A

Abstract

The invention provides a full-automatic light-activated chemiluminescence detector for improving the accuracy of chemical analysis, which comprises a plate taking frame module; a pushing device; a sample addition arm module; a turntable module; a sample rack module; an incubation module; the test device comprises a reagent module and a detection module, wherein the lath on the rack taking module is pushed to the turntable module through the pushing device, a sample and a reagent are added into the reaction cup on the lath on the turntable module through the sample adding arm module, the lath on the turntable module is pushed to enter the incubation module through the pushing device, and the lath enters the detection module to be detected after the incubation is finished. The full-automatic light-activated chemiluminescence detector has the advantages of simple structure, smooth and convenient detection process, high detection efficiency and high detection accuracy.

Description

Full-automatic light-activated chemiluminescence detector

Technical Field

The invention relates to the technical field of medical equipment, in particular to a full-automatic light-activated chemiluminescence detector.

Background

The prior light-activated chemiluminescence detector is generally used for reading a 96-well or 48-well microplate, wherein the 96-well or 48-well microplate comprises 96 or 48 reaction cups, each sample to be detected is placed in one reaction cup, when the number of the samples to be detected is only a few or dozens, a whole 96-well or 48-well microplate is also required to be used, so a great deal of waste is caused, and the time required by the 96-well or 48-well microplate from the first well to the last well in the sample application is longer, so the consistency of the result is influenced to a certain extent, for example, in a patent with an authorization publication number of CN203490229U published in the first 3.19.3.19.A quasi-automatic plate immunoassay analyzer is disclosed, which mainly comprises a box body, a placing unit, an incubation unit, a cleaning unit, a substrate filling unit, an optical detection unit and a system operation control unit, the whole process of reaction liquid incubation, reaction liquid plate washing and reaction liquid self-injection substrate measurement is realized, and two microporous plates can be placed simultaneously during operation. On the other hand, a single cuvette currently in the market is used as a single transfer and detection unit, and detection can be performed when only one sample exists, but the efficiency of single detection is not high enough, and when the number of samples is large, the detection time is long, and the single cuvette is not suitable for large-scale detection, for example, in the patent with the publication number of CN102183639B published in 6.5.2013, a full-automatic chemiluminescence immunoassay analyzer is disclosed, which comprises a cuvette feeding system, a three-dimensional moving sample adding module, a sample area, a reagent area, a pump set and a control system, and the single cuvette is used for transfer detection, so the detection efficiency is not high enough.

The above two detection methods have respective disadvantages, and there is an urgent need for a light-activated chemiluminescent detector between the two detection methods, and the accuracy of the result and the high efficiency of the detection need to be ensured.

Disclosure of Invention

The invention provides a full-automatic light-activated chemical luminescence detector which has the advantages of simple structure, smooth and convenient detection flow, high detection accuracy and high detection efficiency.

The invention discloses a full-automatic light-activated chemiluminescence detector, which comprises a plate taking frame module, a pushing device, a sample adding arm module, a turntable module, a sample frame module, an incubation module, a reagent module and a detection module, wherein the turntable module comprises a turntable base and a turntable body assembly, the turntable body assembly comprises a turntable, a plurality of lath clamping devices are arranged on the turntable, the lath clamping device can place a plurality of laths, the laths on the lath taking module are pushed to the loading area of the turntable module through a Y-direction pushing mechanism of the pushing device, samples and reagents are added into reaction cups on the laths on the loading area of the turntable module through the loading arm module, the X-direction pushing mechanism of the pushing device drives the lath loaded with the mixed liquid on the turntable module to move to the incubation module, and the lath enters the detection module for detection after the incubation is finished;

the rotary disc is positioned behind the plate taking frame module, the incubation module is arranged on one side of the rotary disc, the sample frame module and the reagent module are respectively positioned on two sides of the plate taking frame module, and the incubation is respectively carried out on two incubation plates so as to realize different incubation times.

In one embodiment, the incubation module comprises an incubation plate and a first slide mechanism, the incubation plate is slidably connected with the frame through the first slide mechanism, and a slat clamping device is arranged on the incubation plate.

Preferably, the incubation module includes two incubation plates that are parallel to each other, respectively through a set of first glide machanism and frame sliding connection, first glide machanism includes first motor and first slide rail, the incubation plate is located on first slide rail, first motor through first hold-in range with the incubation plate is connected, first motor rotates and then drives the incubation plate and slides along first slide rail.

In one embodiment, the batten clamping device comprises a clamping bottom plate, vertical plates and batten pressing pieces, wherein the vertical plates are provided with elastic piece clamping grooves, batten elastic pieces are arranged in the elastic piece clamping grooves, the outer side faces of the batten elastic pieces protrude out of the vertical side walls of the vertical plates, the batten pressing pieces are fixed on the upper end faces of the vertical plates and press the batten elastic pieces in the vertical direction, and battens are arranged between every two adjacent vertical plates and are pressed in the horizontal direction through the batten elastic pieces.

In one embodiment, the pushing device comprises an X-direction pushing mechanism and a Y-direction pushing mechanism, the slats on the rack-taking module are pushed onto the carousel module by the Y-direction pushing mechanism, and the slats on the carousel module are pushed into the incubation module by the X-direction pushing mechanism.

In one embodiment, the turntable module comprises a turntable base, a turntable body assembly, a rotating shaft assembly, a turntable motor and an induction assembly, wherein the turntable base is fixed on the rack, the turntable body assembly comprises a turntable, a first gear and a gear pressing sheet, four lath clamping devices which are symmetrically arranged on the same circumference are arranged on the upper surface of the turntable, the rotating shaft assembly penetrates through the gear pressing sheet and the first gear from bottom to top, the upper part of the rotating shaft assembly is fixed on the lower surface of the turntable, a second gear is arranged on the output end of the turntable motor, and the second gear is meshed with the first gear.

In one embodiment, the sensing assembly comprises a turntable zero position sensor and a turntable working position sensor, a convex column is arranged below the batten clamping device on the lower surface of the turntable, the convex column is located on the same circumference with the turntable zero position sensor and the turntable working position sensor, and when the turntable rotates, the lower end of the convex column intermittently passes through the turntable zero position sensor and the turntable working position sensor.

In one embodiment, the plate taking frame module comprises a plate frame for placing the plate strips, a stack, a plate taking frame mechanism and a plate frame transmission mechanism, wherein the plate taking frame mechanism is fixed on the stack through a fixing plate; the plate taking frame mechanism comprises a first plate taking support plate, a second plate taking support plate and a second sliding mechanism, wherein the first plate taking support plate is connected with the second sliding mechanism in a sliding mode through a plate taking connecting plate.

In one embodiment, a rectangular opening is formed in the first plate taking support plate, the second plate taking support plate is horizontally arranged at the rectangular opening in a sliding mode through a screw, a spring is arranged between the first plate taking support plate and the second plate taking support plate, an arc-shaped protruding block is arranged on the outer side of the second plate taking support plate, and the protruding block extends outwards to exceed the outer edge of the first plate taking support plate; be equipped with two brace rods on the lower surface of grillage, the inboard of two brace rods respectively is equipped with a card muscle, and it is sunken that wherein to be equipped with a circular arc on the card muscle that is located lug one side, gets in the board backup pad gets into the storehouse when first board backup pad and the second of getting to when stretching into the below of a grillage of the top, first board backup pad and the second of getting is got the board backup pad and is located between two card muscle, the lug is absorbed in the sunken.

In an embodiment, grillage drive mechanism includes elevator motor, board support, screw rod and two guide arms, and two guide arms and screw rod are parallel to each other and vertically locate storehouse one side, wear to be equipped with the lift slider on two guide arms and the screw rod, the board support is fixed in on the lift slider to inside stretching into the storehouse, the grillage that will place the lath vertically superposes on the board support, superimposed grillage is located inside the storehouse, the lower extreme of screw rod pass through the elevator motor hold-in range with elevator motor is connected.

In one embodiment, the second sliding mechanism includes a second motor and a second slide rail, the board taking connecting plate is disposed on the second slide rail and slides along the second slide rail, the second motor is connected to the board taking connecting plate through a second synchronous belt, and the second motor rotates to drive the board taking connecting plate to slide along the second slide rail.

In one embodiment, the detection module comprises a light path component, a slat transfer component, a detection bottom plate and a third sliding mechanism, wherein a slat falling groove is formed in the detection bottom plate, the slat transfer component is movably arranged on the upper surface of the detection bottom plate, the light path component is arranged on the upper surface of the detection bottom plate through a slat transfer channel, and when detection is carried out, a slat on the slat transfer component is located under the light path component.

In one embodiment, the lath transferring component comprises a sliding block, a lath inserting piece and a direct current motor, wherein a guide rail is arranged on the upper surface of the sliding block, the lath inserting piece is arranged on the guide rail, the direct current motor is arranged on the sliding block, a third gear is arranged at the output end of the direct current motor, a rack is arranged on the lath inserting piece, the third gear and the rack are meshed with each other, the direct current motor rotates to drive the lath inserting piece to slide along the guide rail, a plurality of inserting pieces which are arranged in parallel are arranged on one side, close to the light path component, of the lath inserting piece, and the lath is erected on the inserting pieces.

Preferably, the third sliding mechanism comprises a third motor and a third slide rail, the sliding block is arranged on the third slide rail and slides along the third slide rail, the third motor is connected with the sliding block through a third synchronous belt, and the third motor rotates to drive the sliding block to slide along the third slide rail.

In an embodiment, the sample frame module comprises a sample frame bottom plate, a test tube rack and a test tube rack adapter, a plurality of test tube insertion holes are formed in the test tube rack, test tube clamping pieces are arranged in the test tube insertion holes and used for inserting test tubes of samples into the test tube clamping pieces, a plurality of groups of guide blocks are arranged on the sample frame bottom plate, a guide groove is formed in the bottom surface of the test tube rack adapter and used for fixing the test tube rack into the test tube rack adapter, and the test tube rack adapter are inserted together from one side of the sample frame bottom plate.

Preferably, the front end and the lower surface of the test tube rack adapter are respectively provided with one magnetic steel and two magnetic steels, and the three magnetic steels are all sunk into the interior of the test tube rack adapter.

In one embodiment, pusher still includes the push rod bottom plate, pusher's X is the same to push mechanism and Y to push mechanism structure to respectively through fourth glide machanism and fifth glide machanism and push rod bottom plate sliding connection, X all includes push rod, push rod motor and push rod arm to push mechanism and Y to push mechanism, the push rod arm is improved level and is equipped with the push rod slide rail, the push rod slides and locates on the push rod slide rail to be connected with the push rod motor through the push rod hold-in range.

Preferably, the fourth sliding mechanism includes a fourth motor and a fourth slide rail, the push rod arm is disposed on the fourth slide rail, the fourth motor is connected with the push rod arm through a fourth synchronous belt, the fourth motor rotates to drive the push rod arm to slide along the fourth slide rail, and the fifth sliding mechanism and the fourth sliding mechanism are identical in structure.

In one embodiment, the full-automatic light-activated chemiluminescence detector further comprises a universal liquid module, a liquid path module and a dilution oscillation module, and the sample rack module, the reagent module, the universal liquid module and the dilution oscillation module realize liquid adding operation in the detection process through the liquid path module.

In one embodiment, one needle washing pool is arranged on one side of each of the sample rack module and the reagent module.

Compared with the prior art, the full-automatic light-activated chemiluminescence detector has the advantages that:

the full-automatic light-activated chemiluminescence detector comprises a shell, a rack, slats for detection, a slat taking rack module, a pushing device, a sample adding arm module, a turntable, a sample rack module, an incubation module, a reagent module and a detection module, wherein the slat taking rack module, the pushing device, the sample adding arm module, the turntable, the sample rack module, the incubation module, the reagent module and the detection module are arranged on the rack, the slats for detection are arranged on the slats in parallel, a plurality of slats are stacked in a stack of the slat taking rack module, the uppermost slat is taken out through the slat taking rack mechanism, the slats are pushed to a slat clamping mechanism on the turntable through a Y-direction pushing mechanism, the turntable rotates clockwise for 90 degrees, the slats reach a sample adding position for sample adding and diluting, at the moment, one slat is pushed to the slat clamping mechanism again from the slat clamping mechanism and rotates for 90 degrees again, the sample adding position is used for sample adding, and then the reagent adding operation is carried out after the rotation for two times for 90 degrees, afterwards push the lath into incubation module through X to push mechanism and carry out the incubation, incubation module includes incubation board and first glide machanism, and the lath is fixed in on the incubation board, then makes a round trip to slide through first glide machanism and shake the mixing, carries out the incubation simultaneously and handles, and then detection efficiency is higher, later reentrant detection module detects. Get the grillage module and make things convenient for the grillage to take out and send the carousel in order, the carousel can high-efficiently accomplish the application of sample, add the reagent, dilute the operation such as, sample frame module can be convenient, firm places the sample in the sample district, the realization that can be convenient after the detection module retests is abandoned the lath, and is quick, nimble, and then has guaranteed the high efficiency of detection.

The features mentioned above can be combined in various suitable ways or replaced by equivalent features as long as the object of the invention is achieved.

Drawings

The invention will be described in more detail hereinafter on the basis of non-limiting examples only and with reference to the accompanying drawings. Wherein:

FIG. 1 is a schematic structural diagram of the fully automatic light-activated chemiluminescent detector of the present invention;

FIG. 2 is a schematic structural diagram of the front side of the fully-automatic light-activated chemiluminescent detector of the present invention with the housing removed;

FIG. 3 is a schematic structural view of the reverse side of the fully automatic light-activated chemiluminescent detector of the present invention with the outer shell removed;

FIG. 4 is a schematic view of the slat clamping device according to the present invention;

FIG. 5 is an exploded view of the slat clamping device according to the present invention;

FIG. 6 is a schematic view of the slat clamping device of the present invention shown with the slat hold-down tab removed;

FIG. 7 is a schematic structural diagram of a turntable module according to the present invention;

FIG. 8 is an exploded view of the turntable module of the present invention;

FIG. 9 is an exploded view of the turntable assembly of the present invention;

FIG. 10 is a schematic structural view of a rack-removing module according to the present invention;

FIG. 11 is an exploded view of the rack removal module of the present invention;

FIG. 12 is a schematic structural view of the plate-removing mechanism of the present invention in a plate-removing separated state;

FIG. 13 is a schematic structural view of the plate-taking clamping state of the plate-taking frame mechanism according to the present invention;

FIG. 14 is an exploded view of the pallet retrieval mechanism of the present invention;

FIG. 15 is a schematic structural diagram of a detection module according to the present invention;

FIG. 16 is an exploded view of the detection module of the present invention;

FIG. 17 is a schematic view of the slat transfer assembly of the present invention;

FIG. 18 is a schematic view of the slat insert of the present invention;

fig. 19 is a schematic view showing the structure of the slat transfer passage according to the present invention.

FIG. 20 is a top view of the pushing device of the present invention;

FIG. 21 is a schematic structural diagram of a pushing device according to the present invention;

FIG. 22 is a schematic view of the working state of the Y-direction pushing mechanism according to the present invention;

FIG. 23 is a schematic structural view of the working state of the X-direction pushing mechanism according to the present invention;

FIG. 24 is a schematic view of a sample rack module according to the present invention;

FIG. 25 is an exploded view of the sample rack module of the present invention with the front side of the sample rack base removed;

FIG. 26 is an exploded view of the sample rack module of the present invention with the reverse side of the sample rack base removed.

The corresponding reference numbers for the component names in the figures are as follows:

1. a housing; 2. a frame; 3. a slat; 4. a sample addition arm module; 5. a reagent module; 6. an X-direction pushing mechanism; 7. a Y-direction pushing mechanism; 8. incubating the plate; 9. a first motor; 10. a first slide rail; 11. a first synchronization belt; 12. a vertical plate; 13. a batten compression sheet; 14. a spring plate clamping groove; 15. a lath elastic sheet; 16. a turntable base; 17. a rotating shaft assembly; 18. a turntable motor; 19. a turntable; 20. a first gear; 21. pressing a gear into a sheet; 22. a second gear; 23. a turntable zero position sensor; 24. a turntable working position sensor; 25. a convex column; 26. a plate frame; 27. stacking; 28. a fixing plate; 29. a first plate taking support plate; 30. a second plate taking support plate; 31. taking a plate connecting plate; 32. a rectangular opening; 33. a screw; 34. a spring; 35. a bump; 36. supporting ribs; 37. clamping ribs; 38. recessing; 39. a lifting motor; 40. a plate holder; 41. a screw; 42. a guide bar; 43. a lifting slide block; 44. a second motor; 45. a second slide rail; 46. a second synchronous belt; 47. an optical path component; 48. detecting the bottom plate; 49. the lath falls into the groove; 50. a slat transfer passage; 51. a sliding block; 52. a slat insert; 53. a direct current motor; 54. a guide rail; 55. a third gear; 56. a rack; 57. inserting sheets; 58. a third motor; 59. a third slide rail; 60. a third synchronous belt; 61. a sample rack floor; 62. a test tube rack; 63. a test tube rack adapter; 64. inserting holes of the test tubes; 65. a test tube clamping piece; 66. a guide block; 67. a guide groove; 68. magnetic steel; 69. a push rod bottom plate; 70. a push rod; 71. a push rod motor; 72. a pusher arm; 73. a push rod slide rail; 74. a push rod synchronous belt; 75. a fourth motor; 76. a fourth slide rail; 77. a fourth synchronous belt; 78. a universal liquid module; 79. a liquid path module; 80. a dilution oscillation module; 81. a needle washing pool; 82. a test tube; 83. a plate taking frame module; 84. a pushing device; 85. a turntable module; 86. a sample rack module; 87. an incubation module; 88. a detection module; 89. clamping the bottom plate; 90. a slat clamping device.

In the drawings, like components are denoted by like reference numerals. The figures are not drawn to scale.

Detailed Description

The invention will be described in further detail below with reference to the drawings and specific examples. It should be noted that, as long as there is no conflict, the embodiments and the features of the embodiments of the present invention may be combined with each other, and the technical solutions formed are within the scope of the present invention.

As shown in fig. 1, fig. 2, and fig. 3, the fully automatic light-activated chemiluminescence detector of the present invention comprises a plate taking frame module 83, a pushing device 84, a sample loading arm module 4, a carousel module 85, a sample frame module 86, an incubation module 87, a reagent module 5, and a detection module 88, wherein a plate 3 on the plate taking frame module 83 is pushed by the pushing device 84 onto the carousel module 85, a sample and a reagent are loaded into a cuvette on the plate on the carousel module 85 by the sample loading arm module 4, the plate on the carousel module 85 is pushed by the pushing device 84 into the incubation module 87, and the cuvette is detected by the detection module 88 after the incubation is finished.

In one embodiment, the rack module 83 is disposed at the front of the rack 2, the housing 1 is disposed outside the rack 2, the turntable is disposed behind the rack module 83, the incubation module 87 is disposed at one side of the turntable, the sample rack module 86 and the reagent module 5 are respectively disposed at two sides of the rack module 83, the pushing device 84 includes an X-direction pushing mechanism 6 and a Y-direction pushing mechanism 7, the slat on the rack module 83 is pushed onto the turntable 19 by the Y-direction pushing mechanism 7, the slat 3 on the turntable 19 is pushed into the incubation module 87 by the X-direction pushing mechanism 6, and the detection module 88 detects the slat after the incubation is finished.

In one embodiment, the incubation module 87 comprises an incubation plate 8 and a first slide mechanism by which the incubation plate 8 is slidably connected to the frame 2, the incubation plate 8 being provided with a slat clamping device 90.

In one embodiment, the incubation module 87 comprises two incubation plates 8 arranged in parallel and slidably connected to the frame 2 by a set of first sliding mechanisms, each of the first sliding mechanisms comprises a first motor 9 and a first sliding rail 10, the incubation plates 8 are disposed on the first sliding rail 10, the first motor 9 is connected to the incubation plates 8 by a first synchronous belt 11, and the first motor 9 rotates to drive the incubation plates 8 to slide along the first sliding rail 10.

The incubation divides two incubation boards 8 to go on respectively, can realize different times of incubation respectively to 8 round trip movement's of incubation speed can be decided to the speed of first motor 9 pivoted speed, and then realize the shock mixing of different degrees, operate more nimble changeable.

As shown in fig. 4, 5 and 6, in an embodiment, the slat clamping device 90 includes a clamping bottom plate 89, a vertical plate 12 and a slat pressing sheet 13, the vertical plate 12 is provided with a spring clamping groove 14, a slat spring 15 is provided in the spring clamping groove 14, an outer side surface of the slat spring 15 protrudes out of a vertical side wall of the vertical plate 12, the slat pressing sheet 13 is fixed on an upper end surface of the vertical plate 12 and presses the slat spring 15 in a vertical direction, and the slat 3 is disposed between two adjacent vertical plates 12 and is pressed in a horizontal direction by the slat spring 15. The batten clamping device 90 can clamp the batten 3 in the horizontal direction and the vertical direction at the same time, the batten elastic piece 15 can horizontally clamp the batten 3, and the batten pressing piece 13 can press the batten 3 in the vertical direction, so that the batten 3 is more stable in the moving process.

As shown in fig. 7 to 9, in one embodiment, the turntable module 85 includes a turntable base 16, a tray assembly, a rotating shaft assembly 17, a turntable motor 18 and an induction assembly, the turntable base 16 is fixed on the frame 2, the tray assembly includes a turntable 19, a first gear 20 and a gear pressing sheet 21, four slat clamping devices 90 symmetrically arranged on the same circumference are arranged on the upper surface of the turntable 19, the rotating shaft assembly 17 passes through the gear pressing sheet 21 and the first gear 20 from bottom to top, the upper portion of the rotating shaft assembly 17 is fixed on the lower surface of the turntable 19, a second gear 22 is arranged on the output end of the turntable motor 18, and the second gear 22 is meshed with the first gear 20. Carousel 19 rotates 90 degrees at every turn and has realized the application of sample respectively, has added the diluent, has added operations such as reagent to set up the lath clamping device 90 of a plurality of quantity on the carousel 19, a plurality of laths 3 can be placed to a station, and then have improved detection efficiency.

As shown in fig. 7 and 8, in one embodiment, the sensing assembly includes a turntable zero position sensor 23 and a turntable operating position sensor 24, a stud 25 is disposed on the lower surface of the turntable 19 below the slat clamping device 90, the stud 25 is disposed on the same circumference as the turntable zero position sensor 23 and the turntable operating position sensor 24, and the lower end of the stud 25 intermittently passes through the turntable zero position sensor 23 and the turntable operating position sensor 24 when the turntable 19 rotates. The turntable zero position sensor 23 and the turntable working position sensor 24 record the rotation times of the turntable 19 in real time, and then convert the rotation times into working positions.

As shown in fig. 10-14, in one embodiment, the rack-removing module 83 includes a rack 26 for placing the slats, a stack 27, a rack-removing mechanism and a rack-driving mechanism, the rack-removing mechanism being secured to the stack 27 by a securing plate 28; the plate taking frame mechanism comprises a first plate taking support plate 29, a second plate taking support plate 30 and a second sliding mechanism, wherein the first plate taking support plate 29 is connected with the second sliding mechanism in a sliding mode through a plate taking connecting plate 31.

Preferably, a rectangular opening 32 is formed in the first plate taking support plate 29, the second plate taking support plate 30 is horizontally and slidably arranged at the rectangular opening 32 through a screw 33, a spring 34 is arranged between the first plate taking support plate 29 and the second plate taking support plate 30, an arc-shaped bump 35 is arranged on the outer side of the second plate taking support plate 30, and the bump 35 extends outwards to exceed the outer edge of the first plate taking support plate 29; two support ribs 36 are arranged on the lower surface of the plate frame 26, a clamping rib 37 is arranged on each of the inner sides of the two support ribs 36, a circular arc-shaped recess 38 is formed in the clamping rib 37 positioned on one side of the convex block 35, when the first plate taking support plate 29 and the second plate taking support plate 30 enter the stack 27 and extend into the lower portion of the uppermost plate frame 26, the first plate taking support plate 29 and the second plate taking support plate 30 are positioned between the two clamping ribs 37, and the convex block 35 is recessed into the recess 38. Get the mode that panel frame module 83 blocked calorie of muscle 37 through elastic projection 35 and smoothly take out grillage 26 from storehouse 27 in, when lath 3 all transferred to carousel 19 on, grillage 26 automatic fall collect the frame in, can continue to use next time.

As shown in fig. 11, in one embodiment, the slat support transmission mechanism includes a lifting motor 39, a slat support 40, a screw rod 41, and two guide rods 42, the two guide rods 42 and the screw rod 41 are vertically disposed on one side of the stack 27 in parallel, a lifting slider 43 is disposed on the two guide rods 42 and the screw rod 41, the slat support 40 is fixed on the lifting slider 43 and extends into the stack 27, the slat support 26 on which the slat 3 is disposed is longitudinally stacked on the slat support 40, the stacked slat support 26 is located inside the stack 27, and a lower end of the screw rod 41 is connected to the lifting motor 39 through a lifting motor timing belt (not shown). The plate frames 26 are sequentially stacked in the stack 27, and after the uppermost plate frame 26 is taken out, all the plate frames 26 are lifted to one position through the plate frame transmission mechanism, so that the plate frames 26 in a longitudinal row are sequentially taken out, and then the plate frames 26 in the longitudinal row are manually loaded into the plate frame 26.

Preferably, as shown in fig. 10, the second sliding mechanism includes a second motor 44 and a second slide rail 45, the board taking connecting plate 31 is disposed on the second slide rail 45 and slides along the second slide rail 45, the second motor 44 is connected to the board taking connecting plate 31 through a second timing belt 46, and the second motor 44 rotates to drive the board taking connecting plate 31 to slide along the second slide rail 45. The second slide mechanism allows the rack removal mechanism to move left and right to remove the rack 26 from the stack 27.

As shown in fig. 15 and 16, in one embodiment, the detection module 88 includes an optical path component 47, a slat transfer component, a detection base plate 48, and a third slide mechanism, the detection base plate 48 is provided with a slat drop groove 49, the slat transfer component is movably provided on the upper surface of the detection base plate 48, the optical path component 47 is provided on the upper surface of the detection base plate 48 through a slat transfer passage 50, and a slat 3 on the slat transfer component is positioned directly below the optical path component when detection is performed.

As shown in fig. 15 to 17, the slat transfer assembly includes a sliding block 51, a slat connector 52, and a dc motor 53, a guide rail 54 is disposed on an upper surface of the sliding block 51, the slat connector 52 is disposed on the guide rail 54, the dc motor 53 is disposed on the sliding block 51, a third gear 55 is disposed at an output end of the sliding block, a rack 56 is disposed on the slat connector 52, the third gear 55 and the rack 56 are engaged with each other, the dc motor 53 rotates to drive the slat connector 52 to slide along the guide rail 54, a plurality of insertion pieces 57 disposed in parallel are disposed on a side of the slat connector 52 close to the optical path assembly 47, and the slat 3 is mounted on the insertion pieces 57. The slat 3 to be detected enters the detection module 88 from the slat transfer path 50 side, at this time, the slat connector 52 moves toward the slat transfer path 50, the insertion piece 57 is inserted into the slat 3 from the side, at this time, the slat connector 52 can carry the slat 3 left and right or move back and forth, the slat connector 52 moves the slat 3 right under the optical path component 47 for detection, after the detection is completed, the dc motor 53 rotates to carry the slat connector 52 to move away from the slat transfer path 50, when the slat 3 moves right above the slat drop groove 49, the slat 3 stops moving due to being stopped by the sliding block 51, the slat connector 52 continues to move away from the slat transfer path 50, and the slat connector 52 is separated from the slat 3, at this time, the slat 3 drops downward from the slat drop groove 49.

Preferably, the third sliding mechanism includes a third motor 58 and a third slide rail 59, the sliding block 51 is disposed on the third slide rail 59 and slides along the third slide rail 59, the third motor 58 is connected to the sliding block 51 through a third timing belt 60, and the third motor 58 rotates to drive the sliding block 51 to slide along the third slide rail 59. The third sliding mechanism enables the batten transferring component to move left and right.

As shown in fig. 1 to 3, the pushing device 84 includes an X-direction pushing mechanism 6 and a Y-direction pushing mechanism 7, the strip on the rack-fetching module 83 is pushed to the turntable by the Y-direction pushing mechanism 7, the sample and the reagent are added to the cuvette on the strip 3 on the turntable by the sample-adding arm module, and the strip on the turntable is pushed into the incubation module 87 by the X-direction pushing mechanism 6.

As shown in fig. 20 to 23, in an embodiment, the pushing device 84 further includes a push rod bottom plate 69, the X-direction pushing mechanism 6 and the Y-direction pushing mechanism 7 of the pushing device 84 have the same structure and are slidably connected to the push rod bottom plate 69 through a fourth sliding mechanism and a fifth sliding mechanism, respectively, the X-direction pushing mechanism 6 and the Y-direction pushing mechanism 7 both include a push rod 70, a push rod motor 71 and a push rod arm 72, the push rod arm 72 is horizontally provided with a push rod slide rail 73, and the push rod 70 is slidably disposed on the push rod slide rail 73 and is connected to the push rod motor 71 through a push rod timing belt 74.

Preferably, the fourth sliding mechanism includes a fourth motor 75 and a fourth sliding rail 76, the push rod arm 72 is disposed on the fourth sliding rail 76, the fourth motor 75 is connected to the push rod arm 72 through a fourth synchronous belt 77, the fourth motor 75 rotates to drive the push rod arm 72 to slide along the fourth sliding rail 76, and the fifth sliding mechanism and the fourth sliding mechanism have the same structure.

As shown in fig. 24, the sample rack module 86 includes a sample rack bottom plate 61, a test tube rack 62 and a test tube rack adapter 63, a plurality of test tube insertion holes 64 are formed in the test tube rack 62, test tube clamping sheets 65 are arranged in the test tube insertion holes 64, test tubes 82 for holding samples are inserted into the test tube clamping sheets 65, a plurality of groups of guide blocks 66 are arranged on the sample rack bottom plate 61, a guide groove 67 is formed in the bottom surface of the test tube rack adapter 63, the test tube rack 62 is fixed on the test tube rack adapter 63, and the test tube rack 62 and the test tube rack adapter 63 are inserted together from one side of the sample rack bottom plate 61.

The sample rack module 86 of this configuration is convenient for the user to handle and the test tube 82 for placing the sample is relatively stable. The test tube clamping sheet 65 that sets up in the test-tube rack can be fine the test tube that the clamping fastening has adorned the sample, and then guarantees that the test tube can not incline to place in whole testing process, has guaranteed the reliability and the accuracy of application of sample. The test-tube rack adapter can be conveniently arranged to insert and take out the test-tube rack, and the stability of the test tube is further ensured.

As shown in fig. 25 and 26, in one embodiment, one and two magnetic steels 68 are respectively disposed on the front end and the lower surface of the rack adapter 63, and the three magnetic steels 68 are all sunk into the rack adapter 63. The magnetic steel 68 can play a role in adsorbing the sample rack base plate 61, and further, the test tube rack adapter 63 is more stable.

In one embodiment, the full-automatic light-activated chemiluminescence detector further comprises a universal liquid module 78, a liquid path module 79 and a dilution oscillation module 80, and the sample rack module 86, the reagent module 5, the universal liquid module 78 and the dilution oscillation module 80 realize the operation of adding liquid in the detection process through the liquid path module 79.

In one embodiment, the sample rack module 86 and the reagent module 5 are provided with a wash reservoir 81 on one side. The needle washing pool 81 can wash the sample adding needle on the sample adding arm module 4, and then can be used for multiple times.

The detection method of the full-automatic light-activated chemical luminescence detector is described in detail as follows:

the space occupied by the turntable 19 (fixed in position and not rotating with the rotation of the turntable 19) is divided into a D0 area, a D1 area, a D2 area and a D3 area (the D0 area, the D1 area and the D3 area are shown in fig. 20, and the D2 area is not shown because of being covered by the pushing device 84), wherein the D0 area is used for controlling the rack taking module and the pushing device 84 to load blank slats onto the turntable module 85, and the D1, D2 and D3 areas are used for controlling the turntable module 85 and the sample adding arm module 4 to act, so as to add a solution containing a sample to be detected and a reaction reagent onto the blank slats. The D1 region is also used to complete the operation of adding the diluted sample. The D3 region was used to complete the reagent addition and removal operations. Four slat gripping devices 90 are provided on the turntable 19 to grip the blank slats in the horizontal direction and the vertical direction.

In this embodiment, the first pushing mechanism in the pushing device 84 is the Y-direction pushing mechanism 7, the second pushing mechanism in the moving mechanism is the X-direction pushing mechanism 6, the first predetermined direction is the Y-direction, and the second predetermined direction is the X-direction.

When the detection program is started, the rack taking mechanism in the rack taking module 83 first takes out the rack 26 bearing the blank slats from the stack 27 in the rack taking module 83, and then controls the Y-direction pushing mechanism 7 to drive the blank slats on the rack 26 to move in the Y direction, so that the blank slats move to the position of the turntable 2 corresponding to the D0 area, and are clamped by the slat clamping device 90, so that the slats are more stable in the moving process.

In the rack-removing module 83, the racks 26 are stacked in the stack 27 in sequence, and when the rack-removing mechanism removes one rack 26 from above the stack 27, the rack-driving mechanism drives the stack 27 to move up to a position, i.e., a height between adjacent racks 26. This is done by removing a vertical row of plates 26 and manually loading the plates 26 into the vertical row.

In this embodiment, the sample adding mechanism is the left arm of the sample adding arm in the sample adding arm module 4, and the reagent adding mechanism is the right arm of the sample adding arm in the sample adding arm module 4. The first needle washing pool in the needle washing pool 81 can be used for washing after the left arm of the sample adding arm is added with the solution containing the sample to be detected, and the second needle washing pool in the needle washing pool 81 can be used for washing after the right arm of the sample adding arm is added with the reaction reagent.

After the blank slats are clamped to the turntable 19 by the slat clamping device 90, specifically, the turntable 19 is controlled to rotate so that the blank slats reach the area D1; controlling a left arm of the sample adding arm to add a solution containing a sample to be detected into the blank lath; controlling the turntable 19 to rotate so that the strip added with the solution containing the sample to be measured reaches the area D2; controlling the turntable 19 to rotate so that the strip added with the solution containing the sample to be measured reaches the area D3; and controlling the right arm of the sample adding arm to add a reaction reagent to the strip added with the solution containing the sample to be detected. When the blank plate bar reaches the area D1, the right arm of the sample adding arm is controlled to add additional reaction reagent to the blank plate bar in the area D1. This example does not limit the order of adding the solution containing the sample to be tested and adding the additional reagent. Preferably, additional reaction reagents need to be added before the sample is dispensed, specifically, when the blank panel reaches the area D1, the right arm of the sample application arm is controlled to add such additional reaction reagents into the blank panel of the area D1, and then the left arm of the sample application arm is controlled to add the sample into the blank panel.

In a preferred embodiment, the solution containing the sample to be tested is also diluted before being added to the blank strip. When the blank bar reaches the area D1, controlling the right arm of the sample adding arm to add the pre-dilution liquid into the pre-dilution plate in the dilution oscillation module 80; controlling the left arm of the sample adding arm to add the solution containing the sample to be detected into the pre-dilution plate in the dilution oscillation module 80; the dilution control oscillation module 80 oscillates the pre-dilution plate to obtain a diluted sample; the left arm of the sample application arm is controlled to apply the diluted sample to the blank plate in the D1 area. More preferably, the diluted sample may be added to a portion of the sample area of the blank panel in the region of D1.

Specifically, the sample application process can adopt a multi-sample-absorption combined sample application mode, for example, in the case of n items, wherein only 1 item needs to be pre-diluted, the sample application mechanism absorbs n samples, only 1 sample is distributed into the pre-dilution plate, and n-1 samples are distributed into the blank strip in the area D1. After 1 sample in the pre-dilution plate is diluted, the left arm of the mechanical arm is controlled to add the diluted sample from the pre-dilution plate to the blank bar in the area D1.

When the strip reaches the region D3, the right arm of the mechanical arm is controlled to add one or more reagents to the strip.

The reagents added to the strip were all aqueous solutions.

In this embodiment, the unloading mechanism is controlled to unload the strip carrying the mixed solution of the solution containing the sample to be measured and the reaction reagent from the turntable module 85. Specifically, referring to fig. 2, after the mixing of the solution containing the sample to be measured and the reaction reagent is completed, the unloading mechanism is controlled to unload the slats on the turntable 19 from the slat grippers 90.

Thereafter, the pusher 84 is controlled to move the unloaded stave to the incubation module. Specifically, referring to fig. 2 and fig. 20, the X-direction pushing mechanism 6 is controlled to move the slat carrying the mixed liquor in the X direction, so that the slat carrying the mixed liquor moves to the incubation module 87.

Thereafter, the control incubation module 87 incubates the mixed liquor on the unloaded staves. When the lath carrying the mixed liquor is incubated, a first sliding mechanism in the incubation module 87 is controlled to drive the unloaded lath to slide back and forth so as to uniformly mix the mixed liquor on the lath; the incubation plate 8 in the incubation module 87 is controlled to perform an incubation treatment on the mixture during the tempering treatment.

After the incubation is complete, the moving arm in the control pusher 84 moves the strip carrying the incubated mixture to the detection module 88. After the slat carrying the mixed liquid after incubation enters the detection module 88, the slat transfer component in the detection module 88 is controlled to drive the slat carrying the mixed liquid after incubation to move to the position below the light path component 47 in the detection module 88, and the light path component 47 is controlled to perform laser irradiation on the mixed liquid after incubation.

And then controlling a detection module to perform laser irradiation on the incubated mixed solution and recording the emission light quantity.

In one embodiment, there are multiple incubation modules 87, and the mixture on the unloaded stave is incubated twice using the multiple incubation modules 87 and judged to detect the presence of the high dose-hook effect. Firstly, controlling a first incubation module to perform first-step incubation on the mixed liquor; moving the lath loaded with the mixed solution after the first-step incubation to a detection module; controlling the detection module 88 to perform the first laser irradiation on the mixed solution after the first incubation step and record the emitted light quantity; moving the plate bearing the mixed solution after the first-step reading to a second incubation module; controlling a second incubation module to perform a second incubation on the mixed solution after the first reading; moving the strip carrying the second incubated mixture to detection module 88; the detection module 88 is controlled to perform a second laser irradiation of the mixed solution after the second incubation and record the amount of emitted light. During the first incubation step, the right arm of the sample addition arm is controlled to draw the universal solution from the universal solution module 78 and add it to the incubation mixture.

The control processor determines whether a high dose-hook effect is present based on the amount of light emitted recorded after two incubations. Wherein the control processor determines whether a high dose-hook effect is present based on the amount of light emitted recorded after two incubations, comprising: calculating the difference between the amount of light emitted recorded after the first incubation and the amount of light emitted recorded after the second incubation; judging whether the difference value is larger than a preset threshold value or not; determining that a high dose-hook effect exists when the difference is judged to be greater than a preset threshold; otherwise, in case it is judged that the difference is less than or equal to the preset threshold, it is determined that the high dose-hook effect is not present. Here, the preset threshold is the maximum value of a standard curve measured in the case where the sample to be measured is a standard substance having a concentration lower than that in the presence of the high dose-hook effect. In a preferred embodiment, in the case that the processor determines that the immunoassay has a high dose-hook effect, the dilution oscillation module 80 is controlled to perform dilution processing on the current mixed solution until the high dose-hook effect does not exist.

In one embodiment, the slats 3 for detection are placed on the slat supports 26 in parallel, the plurality of slat supports 26 are stacked in the stack 27 of the slat support module 83, the uppermost slat support 26 is taken out by the slat support mechanism, the slat 3 is pushed to the slat clamping mechanism on the turntable 19 by the Y-direction pushing mechanism 7, the turntable 19 rotates clockwise by 90 degrees, the slat 3 reaches the sample loading position for sample loading and dilution, at this time, one slat 3 is pushed to the slat clamping mechanism again from the slat support 26, the rotation is performed clockwise by 90 degrees, one slat 3 is pushed at each 90 degrees rotation of the pushing position, the sample is loaded at the sample loading position, the sample loading position is loaded with a reagent after the sample loading position rotates twice by 90 degrees, the reagent loading position is loaded with the reagent for reagent loading operation, the slat 3 is pushed to the incubation module 87 by the X-direction pushing mechanism 6 for incubation, and the slat 3 enters the detection module 88 for detection.

The plate taking frame module 83 of the invention is convenient for the plate frame 26 to be taken out and orderly sent into the turntable 19, and the turntable 19 can efficiently finish operations of sample adding, reagent adding, diluting and the like, so that the volume of the whole instrument is greatly reduced, and the detection flow is more efficient. The pushing device 84 comprises an X-direction pushing mechanism 6 and a Y-direction pushing mechanism 7, the lath on the plate taking frame module 83 is pushed to the rotary disc through the Y-direction pushing mechanism 7, a sample and a reagent are added into the reaction cup on the lath 3 on the rotary disc through the sample adding arm module, the lath on the rotary disc is pushed to the incubation module 87 through the X-direction pushing mechanism 6, and the accuracy and the safety of the lath in the conveying process can be guaranteed. The incubation module 87 comprises an incubation plate 8 and a first sliding mechanism, the lath 3 is fixed on the incubation plate 87, then the first sliding mechanism slides back and forth to vibrate and mix uniformly, the incubation treatment is carried out at the same time, the detection efficiency is higher, and then the lath enters the detection module 88 to be detected. The sample rack module 86 can conveniently and stably place the sample in the sample area, and the detection module 88 can conveniently discard the batten 3 after detecting, so that the detection is quick and flexible, and the detection efficiency is further ensured.

While the invention has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the technical features mentioned in the embodiments can be combined in any way as long as no conflict exists. It is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims

1. A full-automatic light-activated chemiluminescence detector is characterized by comprising a plate taking frame module, a pushing device, a sample adding arm module, a turntable module, a sample frame module, an incubation module, a reagent module and a detection module, wherein the turntable module comprises a turntable base and a turntable body assembly, the turntable body assembly comprises a turntable, a plurality of lath clamping devices are arranged on the turntable, the lath clamping device can place a plurality of laths, the laths on the lath taking module are pushed to the loading area of the turntable module through a Y-direction pushing mechanism of the pushing device, samples and reagents are added into reaction cups on the laths on the loading area of the turntable module through the loading arm module, x of the pushing device drives the plate strip loaded with the mixed liquid on the turntable module to move to the incubation module, and the plate strip enters the detection module to be detected after the incubation is finished.

2. The apparatus according to claim 1, wherein the turntable base is fixed to a frame, the turntable assembly further comprises a first gear and a gear pressing plate, four strip clamping devices symmetrically disposed on the same circumference are disposed on the upper surface of the turntable, the rotating shaft assembly passes through the gear pressing plate and the first gear from bottom to top, the upper portion of the rotating shaft assembly is fixed to the lower surface of the turntable, and the output end of the turntable motor is provided with a second gear engaged with the first gear.

3. The apparatus of claim 2, wherein a convex pillar is disposed below the slat clamping device on the lower surface of the turntable, the convex pillar is disposed on the same circumference as the turntable zero position sensor and the turntable operating position sensor, and when the turntable rotates, the lower end of the convex pillar intermittently passes through the turntable zero position sensor and the turntable operating position sensor.

4. The full-automatic light-activated chemiluminescent detector according to claim 1 wherein the rack-removing module comprises racks for placing the racks, a stack, a rack-removing mechanism and a rack transmission mechanism, wherein the rack-removing mechanism is fixed on the stack by a fixing plate; the plate taking frame mechanism comprises a first plate taking support plate, a second plate taking support plate and a second sliding mechanism, wherein the first plate taking support plate is connected with the second sliding mechanism in a sliding mode through a plate taking connecting plate.

5. The full-automatic photoexcitation chemiluminescent detector of claim 4, wherein the first plate-taking support plate is provided with a rectangular opening, the second plate-taking support plate is horizontally slidably provided at the rectangular opening through a screw, a spring is provided between the first plate-taking support plate and the second plate-taking support plate, the outer side of the second plate-taking support plate is provided with a circular arc-shaped projection, and the projection extends outwards beyond the outer edge of the first plate-taking support plate; be equipped with two brace rods on the lower surface of grillage, the inboard of two brace rods respectively is equipped with a card muscle, and it is sunken that wherein to be equipped with a circular arc on the card muscle that is located lug one side, gets in the board backup pad gets into the storehouse when first board backup pad and the second of getting to when stretching into the below of a grillage of the top, first board backup pad and the second of getting is got the board backup pad and is located between two card muscle, the lug is absorbed in the sunken.

6. The apparatus according to claim 1, wherein the detection module comprises a light path component, a slat transfer component, a detection base plate, and a third sliding mechanism, the detection base plate is provided with a slat dropping groove, the slat transfer component is movably disposed on the upper surface of the detection base plate, the light path component is disposed on the upper surface of the detection base plate through a slat transfer channel, and a slat on the slat transfer component is located directly below the light path component when detection is performed.

7. The full-automatic light-activated chemiluminescence detector according to claim 6, wherein the slat transfer assembly comprises a sliding block, a slat inserter and a dc motor, wherein a guide rail is arranged on the upper surface of the sliding block, the slat inserter is arranged on the guide rail, the dc motor is arranged on the sliding block, a third gear is arranged at the output end of the dc motor, a rack is arranged on the slat inserter, the third gear and the rack are meshed with each other, the dc motor rotates to drive the slat inserter to slide along the guide rail, a plurality of mutually parallel inserts are arranged on one side of the slat inserter close to the light path assembly, and the slat is arranged on the inserts.

8. The apparatus according to claim 7, wherein the third sliding mechanism comprises a third motor and a third slide rail, the sliding block is disposed on the third slide rail and slides along the third slide rail, the third motor is connected to the sliding block via a third synchronous belt, and the third motor rotates to drive the sliding block to slide along the third slide rail.

9. The full-automatic light-activated chemical luminescence detector of claim 1, wherein the pushing device further comprises a push rod bottom plate, the X-direction pushing mechanism and the Y-direction pushing mechanism of the pushing device have the same structure and are slidably connected with the push rod bottom plate through a fourth sliding mechanism and a fifth sliding mechanism respectively, the X-direction pushing mechanism and the Y-direction pushing mechanism respectively comprise a push rod, a push rod motor and a push rod arm, a push rod sliding rail is horizontally arranged on the push rod arm, and the push rod is slidably arranged on the push rod sliding rail and is connected with the push rod motor through a push rod synchronous belt.

10. The full-automatic light-activated chemical luminescence detector of claim 9, wherein the fourth sliding mechanism comprises a fourth motor and a fourth sliding rail, the push rod arm is disposed on the fourth sliding rail, the fourth motor is connected to the push rod arm through a fourth synchronous belt, the fourth motor rotates to drive the push rod arm to slide along the fourth sliding rail, and the fifth sliding mechanism and the fourth sliding mechanism have the same structure.