CN202735344U - Immunochromatographic detection device - Google Patents
Immunochromatographic detection device Download PDFInfo
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- CN202735344U CN202735344U CN 201220355733 CN201220355733U CN202735344U CN 202735344 U CN202735344 U CN 202735344U CN 201220355733 CN201220355733 CN 201220355733 CN 201220355733 U CN201220355733 U CN 201220355733U CN 202735344 U CN202735344 U CN 202735344U
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Abstract
An immunochromatographic detection device comprises reagent strips and a bottom plate, wherein at least two reagent strips or cards are arranged on the same bottom plate; the reagent strips respectively comprise upper sample pads, marker pads, nitrocellulose membranes, water absorbing papers and lining plates; the upper sample pads, the marker pads, the nitrocellulose membranes and the water absorbing papers are spliced with one another and bonded on the lining plates; and detection lines and quality control lines are coated on the nitrocellulose membranes. In the immunochromatographic detection device provided by the utility model, the two reagent strips or cards can be arranged on the bottom plate to form a duplex detecting device and can be also bonded with a plurality of reagent strips or cards to form a multi-connected detecting device; the sample is simultaneously detected through the adoption of the different reagent strips, so that antibodies or antigens in blood and urine can be quantitatively, semi-quantitatively or qualitatively detected; errors caused by sample dilution aimed to avoid hook effect due to overhigh sample concentration in the using process of the traditional detection device are overcome; the possibility of false negative caused in clinical diagnosis is eliminated; and loss caused to patients is avoided.
Description
Technical field
The utility model relates to the technical field of medicine equipment in-vitro diagnosis device, particularly a kind ofly can overcome hook effect, has the immuno-chromatography detection device of duplex or multi-joint reagent strip.
Background technology
Hook effect refers in the immune detection owing to antigen, the improper testing result that causes of antibody concentration ratio are false-negative phenomenon.Nineteen twenty-nine Heidelberger utilizes equivalent antibody test concentration to increase progressively antigen, and when antigen concentration is lower, when antibody concentration was relatively high, precipitation reaction was not obvious; When antigen concentration is increased to when suitable with the antibody concentration ratio, precipitation reaction is obvious; When continuing to increase antigen concentration, precipitation reaction weakens on the contrary.Draw accordingly the two-phase response curve, zone, curve peak, antibody, antigen concentration are optimum ratio, and precipitation reaction is obvious, claims equivalence zone.Left side, zone, peak, because antibody concentration is too high, precipitation reaction is not obvious, band before claiming; Right side, zone, peak.Because antigen concentration is too high, precipitation reaction is also not obvious, band after claiming.The result claimed prozone phenomenon due to antibody concentration was too high, and the result claimed Postzone phenomenon due to antigen concentration was too high, was referred to as zoning.Green called hook effect (hook effect) to this phenomenon in 1977, had comprised the front and back belt phenomenon.
The reason of hook effect once had multiple explanation.In conjunction with reason immunologic achievement of generation and electron microscopic observation finding, the lattice theory that available Marrack proposes is illustrated.Because most of antibody be divalence and antigen is multivalence, when the amount of antigen-antibody is in equivalence zone, can interconnect to become and have the huge with the trellis aggregation of spatial structure, form meat and limit visible anti-shallow lake thing, but when antigen or antibody excess, because the valency of surplus side can not get saturated, to affect to some extent the integrality of the structure of immune complex (IC), can only form less sediment or the IC of solubility, or reversible dissolution occurs, the network that is IC is more imperfect, and dissolving or reversible dissociating more easily occur.For the immunity test of various marks, then be because of the IC of the IC that formed solubility or formation easily from the solid phase carrier Xian take off.In single stage method, excessive measured object is combined respectively with solid phase albumen and labelled protein, cannot or hardly effectively form sandwich complex.Hook effect can make strong positive sample mistake survey as the weak positive even cause false-negative result, and its direct result is the state of an illness that misleads clinical diagnosis and incur loss through delay the patient.
Since hook effect can produce adverse consequences like this, eliminate as far as possible hook effect and seem very necessary.At present the most frequently used method is the sample dilution, but the sample dilution tends to bring very large trouble to the reviewer, even error.
Summary of the invention
The purpose of this utility model is in order to address the above problem, and has designed a kind ofly can overcome hook effect, has the immuno-chromatography detection device of duplex or multi-joint reagent strip.
The technical scheme that the utility model is taked for the technical matters that exists in the solution known technology is:
Immuno-chromatography detection device of the present utility model comprises reagent strip and base plate, at same base plate at least two reagent strips is set; The mentioned reagent bar comprises respectively loading pad, labeling pad, nitrocellulose membrane, thieving paper and liner plate; Loading pad, labeling pad, nitrocellulose membrane, thieving paper overlap successively and arrange and sticks on the liner plate, are coated with detection line and nature controlling line at nitrocellulose membrane
Described immuno-chromatography detection device comprises also that the card of moulding of hollow, mentioned reagent bar place respectively independently to mould Ka Nei and moulded card encirclement, moulds between card and the base plate mutually bonding.
In the immuno-chromatography detection device of the present utility model, two reagent strips can be set on base plate, consist of the duplex pick-up unit, also can paste a plurality of reagent strips, make multi-joint pick-up unit, simultaneously sample is detected by different reagent strips, thereby can be quantitative, in sxemiquantitative or the qualitative detection blood, antibody or antigen in the urine, overcome existing pick-up unit and in use diluted the error that causes for the sample that the hook effect of avoiding the too high generation of concentration of specimens carries out, eliminated and in clinical diagnosis, caused false-negative possibility, avoided causing beyond thought loss to the patient.
Description of drawings
Fig. 1 is the structural representation of immuno-chromatography detection device of the present utility model;
Fig. 2 is the structural representation of reagent strip in the immuno-chromatography detection device of the present utility model.
Reference numeral: 1 base plate, 2 is moulded card, 3 nature controlling lines, 4 detection lines, 5 sample aperture, 6 thieving papers, 7 nitrocellulose membranes, 8 labeling pad, 9 loading pads, 10 liner plates.
Embodiment
Below in conjunction with accompanying drawing the utility model is specifically described, Fig. 1 is the structural representation of immuno-chromatography detection device of the present utility model; Fig. 2 is the structural representation of reagent strip in the immuno-chromatography detection device of the present utility model.
As depicted in figs. 1 and 2, immuno-chromatography detection device of the present utility model comprises reagent strip and base plate 1, at same base plate at least two reagent strips is set; The mentioned reagent bar comprises respectively loading pad 9, labeling pad 8, nitrocellulose membrane 7, thieving paper 6 and liner plate 10; Loading pad 9, labeling pad 8, nitrocellulose membrane 7, thieving paper 6 overlap successively and arrange and sticks on the liner plate 10, are coated with detection line 4 and nature controlling line 3 at nitrocellulose membrane.
What also comprise hollow in the immuno-chromatography detection device moulds card 2, and the mentioned reagent bar places respectively independently to be moulded Ka Nei and moulded card encirclement, moulds between card 2 and the base plate 1 mutually bonding; Mould that card is upper to be arranged corresponding to the reagent surveyed area of the cellulose nitrate film location on the reagent strip with corresponding to the sample aperture 5 of the loading pad position of reagent strip.
Take the reagent strip that detects human chorionic gonadotrophin (HCG) as example, Jiang Gao district is installed on respectively different moulding in the card with the low HCG reagent strip of distinguishing, and then will mould card 2 and stick on the base plate 1, moulds and indicates the reagent surveyed area on the card 2; The reagent strip structure comprises loading pad 9, labeling pad 8, nitrocellulose membrane 7, thieving paper 6 and liner plate 10, loading pad, labeling pad, nitrocellulose membrane, thieving paper stick on the liner plate successively, are coated with simultaneously the nature controlling line 3 that detection line 4 that the mouse-anti HCG-α of variable concentrations consists of and sheep anti-mouse igg antibody consist of on the nitrocellulose membrane.
Immuno-chromatography detection device of the present utility model cooperates the immunochromatography interpretoscope to use when quantitatively detecting, and concrete steps are as follows: take out immuno-chromatography detection device from refrigerator, and balance is to room temperature; Immuno-chromatography detection device is kept flat, add 80ul sample to be checked, interpretation on interpretoscope after 10 minutes at the place, sample aperture center of HCG-L (reagent is detected in low district) and HCG-H (reagent is detected in high district) respectively with sample loading gun; During such as test concentrations value demonstration≤1000mIU/ml, this sample concentration value is HCG-L test card test concentrations value; When test concentrations value demonstration＞1000mIU/ml, this sample concentration value is as the criterion with HCG-H test card test concentrations value.
Technique scheme has only embodied the optimal technical scheme of technical solutions of the utility model; some changes that those skilled in the art may make some part have wherein all embodied principle of the present utility model, belong within the protection domain of the present utility model.
Claims (2)
1. an immuno-chromatography detection device comprises reagent strip and base plate, it is characterized in that: at same base plate at least two reagent strips are set; The mentioned reagent bar comprises respectively loading pad, labeling pad, nitrocellulose membrane, thieving paper and liner plate; Loading pad, labeling pad, nitrocellulose membrane, thieving paper overlap successively and arrange and sticks on the liner plate, are coated with detection line and nature controlling line at nitrocellulose membrane.
2. immuno-chromatography detection device according to claim 1 is characterized in that: also comprise the card of moulding of hollow, the mentioned reagent bar places respectively independently to be moulded Ka Nei and is moulded card encirclement, moulds between card and the base plate mutually bonding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220355733 CN202735344U (en) | 2012-07-23 | 2012-07-23 | Immunochromatographic detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220355733 CN202735344U (en) | 2012-07-23 | 2012-07-23 | Immunochromatographic detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202735344U true CN202735344U (en) | 2013-02-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220355733 Expired - Lifetime CN202735344U (en) | 2012-07-23 | 2012-07-23 | Immunochromatographic detection device |
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| CN (1) | CN202735344U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543272A (en) * | 2013-10-17 | 2014-01-29 | 天津中新科炬生物制药有限公司 | Rapid and quantitative detection device and method for simultaneously detecting heart-type fatty acid-binding protein and cardiac troponin I |
| CN105628939A (en) * | 2015-08-21 | 2016-06-01 | 蓝十字生物药业（北京）有限公司 | Grade rapid semi-quantitative ovulation colloidal gold test paper, kit and detection method |
| CN112285358A (en) * | 2020-09-17 | 2021-01-29 | 上海基灵生物科技有限公司 | Reagent card for dog C-reactive protein and pancreas specific lipase duplex detection |
| CN114675043A (en) * | 2017-03-28 | 2022-06-28 | 科美诊断技术股份有限公司 | Control method of immunoassay device |
-
2012
- 2012-07-23 CN CN 201220355733 patent/CN202735344U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543272A (en) * | 2013-10-17 | 2014-01-29 | 天津中新科炬生物制药有限公司 | Rapid and quantitative detection device and method for simultaneously detecting heart-type fatty acid-binding protein and cardiac troponin I |
| CN105628939A (en) * | 2015-08-21 | 2016-06-01 | 蓝十字生物药业（北京）有限公司 | Grade rapid semi-quantitative ovulation colloidal gold test paper, kit and detection method |
| CN114675043A (en) * | 2017-03-28 | 2022-06-28 | 科美诊断技术股份有限公司 | Control method of immunoassay device |
| CN112285358A (en) * | 2020-09-17 | 2021-01-29 | 上海基灵生物科技有限公司 | Reagent card for dog C-reactive protein and pancreas specific lipase duplex detection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Immuno-chromatography detection device and detection method thereof Effective date of registration: 20150204 Granted publication date: 20130213 Pledgee: Tianjin TEDA Small Enterprise Credit Guarantee Center Pledgor: Tianjin NewScen Coast Bio-Pharmaceutical Co.,Ltd. Registration number: 2015120000009 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20160307 Granted publication date: 20130213 Pledgee: Tianjin TEDA Small Enterprise Credit Guarantee Center Pledgor: Tianjin NewScen Coast Bio-Pharmaceutical Co.,Ltd. Registration number: 2015120000009 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 300457 Tianjin Binhai New Area, Tanggu Development Zone, No. sixth main street, No. 65 Patentee after: NEWSCEN COAST BIO PHARMACEUTICAL CO.,LTD. Address before: 300457 Tianjin Binhai New Area, Tanggu Development Zone, No. sixth main street, No. 65 Patentee before: Tianjin NewScen Coast Bio-Pharmaceutical Co.,Ltd. |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130213 |